SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_02905 FitnessBrowser__Burk376:H281DRAFT_02905 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
24% identity, 86% coverage: 1:213/247 of query aligns to 1:229/291 of 5odqB

query
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5odqB

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binding Non-cubane [4Fe-4S]-cluster: G8 (≠ P8), C9 (= C9), C41 (= C41), C42 (= C42), C78 (vs. gap), G80 (vs. gap), C81 (vs. gap), G152 (≠ S133), C153 (= C134), H154 (≠ R138), C193 (= C177), C194 (= C178)
binding 2-bromanylethanesulfonic acid: G46 (= G47), G80 (vs. gap), H154 (≠ R138), G197 (= G181), R201 (≠ S185)

Sites not aligning to the query:

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
24% identity, 86% coverage: 1:213/247 of query aligns to 1:229/291 of 5odhH

query
sites
5odhH

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binding Non-cubane [4Fe-4S]-cluster: G8 (≠ P8), C9 (= C9), C41 (= C41), C42 (= C42), C78 (vs. gap), G80 (vs. gap), C81 (vs. gap), C153 (= C134), H154 (≠ R138), C193 (= C177), C194 (= C178)
binding 1-thioethanesulfonic acid: A44 (≠ Q44), P45 (= P45), G46 (= G47), G80 (vs. gap), H154 (≠ R138), G197 (= G181), G198 (= G182)
binding Coenzyme B: R201 (≠ S185)

Sites not aligning to the query:

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
24% identity, 86% coverage: 1:213/247 of query aligns to 1:229/291 of 5odhB

query
sites
5odhB

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binding Non-cubane [4Fe-4S]-cluster: G8 (≠ P8), C9 (= C9), I10 (≠ F10), C41 (= C41), C42 (= C42), C78 (vs. gap), G80 (vs. gap), C81 (vs. gap), G152 (≠ S133), C153 (= C134), H154 (≠ R138), C193 (= C177), C194 (= C178)
binding 1-thioethanesulfonic acid: P45 (= P45), G46 (= G47), G80 (vs. gap)

Sites not aligning to the query:

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
24% identity, 86% coverage: 1:213/247 of query aligns to 1:229/291 of 5odcB

query
sites
5odcB

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binding Non-cubane [4Fe-4S]-cluster: G8 (≠ P8), C9 (= C9), I10 (≠ F10), C41 (= C41), C42 (= C42), C78 (vs. gap), N79 (vs. gap), G80 (vs. gap), C81 (vs. gap), G152 (≠ S133), C153 (= C134), C193 (= C177), C194 (= C178), G197 (= G181)

Sites not aligning to the query:

binding Non-cubane [4Fe-4S]-cluster: 231, 233, 234

Query Sequence

>H281DRAFT_02905 FitnessBrowser__Burk376:H281DRAFT_02905
MKVALFIPCFIDAFYPEVGIATLELLERFGIQVDYPQEQTCCGQPMGNSGAHAEAAGAER
VFARNFAGYDYIVGPSASCIHHVREHLTAIDQTDEVKKVRASAYELVEFLHDVVGAREFP
WAEFPYRVGLHNSCSALRHLKEASISEVAGQPFSKPRALLEGVKGIEFVKPSRPDECCGF
GGTFSVTEEPVSVRMGQDKVRDHLNAGAQYIVSGDMSCLMHQQGCAERMKADARFIHIAQ
VLNGACA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory