SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_03179 FitnessBrowser__Burk376:H281DRAFT_03179 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
69% identity, 98% coverage: 3:423/429 of query aligns to 1:421/421 of P50457

query
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P50457

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K267 (= K269) mutation to A: No GABA-AT activity.

P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
54% identity, 99% coverage: 1:424/429 of query aligns to 1:424/426 of P22256

query
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P22256

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I50 (= I51) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
GS 111:112 (≠ GA 112:113) binding pyridoxal 5'-phosphate
E211 (= E212) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
V241 (= V242) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
Q242 (= Q243) binding pyridoxal 5'-phosphate
K268 (= K269) modified: N6-(pyridoxal phosphate)lysine
T297 (= T298) binding pyridoxal 5'-phosphate

1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
54% identity, 98% coverage: 4:424/429 of query aligns to 2:423/425 of 1sffA

query
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1sffA

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A

active site: V18 (= V20), Y137 (≠ F139), E205 (= E207), D238 (= D240), Q241 (= Q243), K267 (= K269), T296 (= T298), R397 (= R398)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (= Q80), G110 (= G112), S111 (≠ A113), Y137 (≠ F139), H138 (= H140), R140 (= R142), E205 (= E207), D238 (= D240), V240 (= V242), Q241 (= Q243), K267 (= K269), T296 (= T298)
binding sulfate ion: N152 (≠ A154), Y393 (≠ S394)

1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
54% identity, 98% coverage: 4:424/429 of query aligns to 2:423/425 of 1sf2A

query
sites
1sf2A

K

S

N

N

A

K

E

E

L

L

K

M

S

Q

R

R

K

R

D

S

A

Q

A

A

T

I

P

P

R

R

G

G

V
|
V

G

G

V

Q

M

I

C

H

D

P

F

I

Y

F

A

A

E

D

R

R

A

A

Q

E

N

N

A

C

E

R

L

V

W

W

D

D

V

V

E

E

G

G

R

R

R

E

F

Y

I

L

D

D

F

F

A

A

A

G

G

G

I

I

A

A

V

V

C

L

N

N

T

T

G

G

H

H

R

L

H

H

P

P

K

K

I

V

V

V

A

A

I

V

R

E

D

A

Q

Q

L

L

D

K

R

K

F

L

T

S

H

H

T

T

A

C

Y

F

Q

Q

I

V

V

L

P

A

Y

Y

A

E

S

P

Y

Y

V

L

E

E

L

L

A

C

E

E

K

I

L

M

N

N

E

Q

R

K

A

V

P

P

G

G

D

D

H

F

P

A

K

K

T

T

A

L

F

L

F

V

T

T

G
|
G

A
x
S

E

E

A

A

V

V

E

E

N

N

A

A

I

V

K

K

I

I

A

A

R

R

A

A

T

T

G

K

R

R

P

S

G

G

V

T

I

I

A

A

F

F

T

S

G

G

G

A

F
x
Y

H
|
H

G

G

R

R

T

T

L

H

M

Y

G

T

M

L

A

A

L

L

T

T

G

G

K

K

V

V

A
x
N

P

P

Y

Y

K

S

A

A

G

G

F

M

G

G

P

L

F

M

P

P

S

G

D

H

V

V

F

Y

H

R

A

A

P

L

F

Y

P

P

N

C

P

P

L

L

H

H

G

G

V

I

S

S

T

E

A

D

D

D

S

A

L

I

K

A

A

S

I

I

E

H

F

R

L

I

F

F

K

K

A

N

D

D

I

A

D

A

P

P

K

E

R

D

V

I

A

A

I

I

I

V

F

I

E
|
E

P

P

V

V

Q

Q

G

G

E

E

G

G

F

F

Y

Y

P

A

A

S

P

S

A

P

E

A

F

F

V

M

R

Q

A

R

L

L

R

R

K

A

L

L

C

C

N

D

E

E

H

H

G

G

I

I

L

M

L

L

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

S

G

G

F

A

A

G

R

R

T

T

G

G

K

T

L

L

F

F

A

A

M

M

H

E

H

Q

Y

M

D

G

V

V

V

A

P

P

D

D

L

L

M

T

T

T

V

F

A

A

K
|
K

S

S

L

I

A

A

G

G

M

F

P

P

L

L

S

A

G

G

V

V

I

T

G

G

R

R

A

A

D

E

V

V

M

M

D

D

A

A

A

V

A

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

A

A

G

G

N

N

P

P

L

I

A

A

V

C

A

V

A

A

L

L

A

E

V

V

L

L

D

K

I

V

I

F

D

E

E

Q

E

E

K

N

L

L

C

L

E

Q

R

K

A

A

T

N

I

D

L

L

G

G

D

Q

R

K

V

L

K

K

A

D

K

G

L

L

I

L

A

A

L

I

Q

A

N

E

E

K

A

H

P

P

Q

E

I

I

A

G

D

D

V

V

R

R

G

G

P

L

G

G

G

A

M

M

V

I

A

A

V

I

E

E

F

L

C

F

K

E

P

D

G

G

S

D

-

H

T

N

E

K

P

P

D

D

A

A

E

K

F

L

T

T

K

A

R

E

V

I

Q

V

T

A

R

R

A

A

L

R

K

D

R

K

G

G

L

L

L

I

L

L

V

S

C

C

G

G

V

P

Y

Y

S
x
Y

N

N

V

V

V

L

R
|
R

F

I

L

L

F

V

P

P

L

L

T

T

I

I

E

E

D

D

T

A

V

Q

F

I

D

R

E

Q

A

G

L

L

A

E

I

I

L

I

E

S

D

Q

V

C

I

F

K

D

D

E

S

A

active site: V18 (= V20), Y137 (≠ F139), E205 (= E207), D238 (= D240), Q241 (= Q243), K267 (= K269), T296 (= T298), R397 (= R398)
binding pyridoxal-5'-phosphate: G110 (= G112), S111 (≠ A113), Y137 (≠ F139), H138 (= H140), E205 (= E207), D238 (= D240), V240 (= V242), Q241 (= Q243), K267 (= K269)
binding sulfate ion: N152 (≠ A154), Y393 (≠ S394)

1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
54% identity, 98% coverage: 4:424/429 of query aligns to 2:423/425 of 1szkA

query
sites
1szkA

K

S

N

N

A

K

E

E

L

L

K

M

S

Q

R

R

K

R

D

S

A

Q

A

A

T

I

P

P

R

R

G

G

V
|
V

G

G

V

Q

M

I

C

H

D

P

F

I

Y

F

A

A

E

D

R

R

A

A

Q

E

N

N

A

C

E

R

L

V

W

W

D

D

V

V

E

E

G

G

R

R

R

E

F

Y

I

L

D

D

F

F

A

A

A

G

G

G

I

I

A

A

V

V

C

L

N

N

T

T

G

G

H

H

R

L

H

H

P

P

K

K

I

V

V

V

A

A

I

V

R

E

D

A

Q

Q

L

L

D

K

R

K

F

L

T

S

H

H

T

T

A

C

Y

F

Q

Q

I

V

V

L

P

A

Y

Y

A

E

S

P

Y

Y

V

L

E

E

L

L

A

C

E

E

K

I

L

M

N

N

E

Q

R

K

A

V

P

P

G

G

D

D

H

F

P

A

K

K

T

T

A

L

F

L

F

V

T

T

G
|
G

A
x
S

E

E

A

A

V

V

E

E

N

N

A

A

I

V

K

K

I

I

A

A

R

R

A

A

T

T

G

K

R

R

P

S

G

G

V

T

I

I

A

A

F

F

T

S

G

G

G

A

F
x
Y

H
|
H

G

G

R

R

T

T

L

H

M

Y

G

T

M

L

A

A

L

L

T

T

G

G

K

K

V

V

A

N

P

P

Y

Y

K

S

A

A

G

G

F

M

G

G

P

L

F

M

P

P

S

G

D

H

V

V

F

Y

H

R

A

A

P

L

F

Y

P

P

N

C

P

P

L

L

H

H

G

G

V

I

S

S

T

E

A

D

D

D

S

A

L

I

K

A

A

S

I

I

E

H

F

R

L

I

F

F

K

K

A

N

D

D

I

A

D

A

P

P

K

E

R

D

V

I

A

A

I

I

I

V

F

I

E
|
E

P

P

V

V

Q

Q

G

G

E

S

G

G

F

F

Y

Y

P

A

A

S

P

S

A

P

E

A

F

F

V

M

R

Q

A

R

L

L

R

R

K

A

L

L

C

C

N

D

E

E

H

H

G

G

I

I

L

M

L

L

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

S

G

G

F

A

A

G

R

R

T

T

G

G

K

T

L

L

F

F

A

A

M

M

H

E

H

Q

Y

M

D

G

V

V

V

A

P

P

D

D

L

L

M

T

T

T

V

F

A

A

K
|
K

S

S

L

I

A

A

G

G

M

F

P

P

L

L

S

A

G

G

V

V

I

T

G

G

R

R

A

A

D

E

V

V

M

M

D

D

A

A

A

V

A

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

A

A

G

G

N

N

P

P

L

I

A

A

V

C

A

V

A

A

L

L

A

E

V

V

L

L

D

K

I

V

I

F

D

E

E

Q

E

E

K

N

L

L

C

L

E

Q

R

K

A

A

T

N

I

D

L

L

G

G

D

Q

R

K

V

L

K

K

A

D

K

G

L

L

I

L

A

A

L

I

Q

A

N

E

E

K

A

H

P

P

Q

E

I

I

A

G

D

D

V

V

R

R

G

G

P

L

G

G

G

A

M

M

V

I

A

A

V

I

E

E

F

L

C

F

K

E

P

D

G

G

S

D

-

H

T

N

E

K

P

P

D

D

A

A

E

K

F

L

T

T

K

A

R

E

V

I

Q

V

T

A

R

R

A

A

L

R

K

D

R

K

G

G

L

L

L

I

L

L

V

S

C

C

G

G

V

P

Y

Y

S

Y

N

N

V

V

V

L

R
|
R

F

I

L

L

F

V

P

P

L

L

T

T

I

I

E

E

D

D

T

A

V

Q

F

I

D

R

E

Q

A

G

L

L

A

E

I

I

L

I

E

S

D

Q

V

C

I

F

K

D

D

E

S

A

active site: V18 (= V20), Y137 (≠ F139), E205 (= E207), D238 (= D240), Q241 (= Q243), K267 (= K269), T296 (= T298), R397 (= R398)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G112), S111 (≠ A113), Y137 (≠ F139), H138 (= H140), E205 (= E207), D238 (= D240), V240 (= V242), Q241 (= Q243), K267 (= K269)

3q8nC Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis (see paper)
50% identity, 97% coverage: 7:423/429 of query aligns to 19:437/439 of 3q8nC

query
sites
3q8nC

E

E

L

L

K

Q

S

A

R

R

K

K

D

Q

A

S

A

A

T

V

P

A

R

A

G

G

V
|
V

G

G

V

V

M

T

C

L

D

P

F

V

Y

Y

A

V

E

V

R

A

A

A

Q

G

N

G

A

G

E

V

L

L

W

A

D

D

V

A

E

D

G

G

R

N

R

Q

F

L

I

I

D

D

F

F

A

G

A

S

G

G

I

I

A

A

V

V

C

T

N

T

T

V

G

G

H

N

R

S

H

A

P

P

K

A

I

V

V

V

A

D

A

A

I

V

R

T

D

Q

Q

Q

L

V

D

A

R

A

F

F

T

T

H

H

T

T

A

C

Y

F

Q

M

I

V

V

T

P

P

Y

Y

A

E

S

G

Y

Y

V

V

E

K

L

V

A

A

E

E

K

H

L

L

N

N

E

R

R

L

A

T

P

P

G

G

D

D

H

H

P

E

K

K

K

R

T

T

A

A

F

L

F

F

T

N

T

S

G
|
G

A
|
A

E

E

A

A

V

V

E

E

N

N

A

A

I

V

K

K

I

I

A

A

R

R

A

A

A

Y

T

T

G

R

R

R

P

Q

G

A

V

V

I

V

A

V

F

F

T

D

G

H

G

A

F
x
Y

H

H

G

G

R

R

T

T

L

N

M

L

G

T

M

M

A

A

L

M

T

T

G

A

K

K

V

N

A

Q

P

P

Y

Y

K

K

A

H

G

G

F

F

G

G

P

P

F

F

P

A

S

N

D

E

V

V

F

Y

H

R

A

V

P

P

F

T

P

S

N

Y

P

P

L

F

H

R

G

D

V

G

S

E

T

T

A

D

D

G

S

A

L

A

K

A

A

A

I

A

E

H

F

A

L

L

-

D

-

L

F

I

K

N

A

K

D

Q

I

V

D

G

P

A

K

D

R

N

V

V

A

A

I

V

F

I

E
|
E

P

P

V

V

Q

H

G

G

E

E

G

G

F

F

Y

V

-

V

P

P

A

A

P

P

A

G

E

-

F

F

V

L

R

G

A

A

L

L

R

Q

K

K

L

W

C

C

N

T

E

D

H

N

G

G

I

A

L

V

L

F

I

V

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

F

F

A

A

R

R

T

T

G

G

K

A

L

L

F

F

A

A

M

C

H

E

H

H

Y

E

D

N

V

V

P

P

D

D

L

L

M

I

T

V

V

T

A

A

K
|
K

S

G

L

I

A

A

G

G

M

L

P

P

L

L

S

S

G

A

V

V

I

T

G

G

R

R

A

A

D

E

V

I

M

M

D

D

A

G

A

P

A

Q

P

S

G

G

L

L

G

G

T
|
T

Y

Y

A

G

G

G

N

N

P

P

L

L

A

A

V

C

A

A

L

L

A

A

V

V

L

I

D

D

I

T

I

I

D

E

E

R

E

E

K

N

L

L

C

V

E

A

R

R

A

A

T

R

I

A

L

I

G

G

D

E

R

T

V

M

K

L

A

S

K

R

L

L

I

G

A

A

L

L

Q

A

N

A

E

A

A

D

P

P

Q

R

I

I

A

G

D

E

V

V

R

R

G

G

P

R

G

G

G

A

M

M

V

I

A

A

V

V

E

E

F

L

C

V

K

K

P

P

G

G

S

T

T

T

E

E

P

P

D

D

A

A

E

D

F

L

T

T

K

K

R

R

V

V

Q

A

T

A

R

A

A

A

L

H

K

A

R

Q

G

G

L

L

L

V

L

L

V

T

C

C

G

G

V

T

Y

Y

S

G

N

N

V

V

V

L

R
|
R

F

F

L

L

F

P

P

P

L

L

T

S

I

M

E

P

D

D

T

H

V

L

F

L

D

D

E

E

A

G

L

L

A

D

I

I

L

L

E

A

D

A

V

V

I

F

K

A

D

E

active site: V32 (= V20), Y151 (≠ F139), E221 (= E207), D254 (= D240), Q257 (= Q243), K283 (= K269), T312 (= T298), R412 (= R398)
binding 4-oxobutanoic acid: G124 (= G112), A125 (= A113), V256 (= V242), K283 (= K269)

4atqF Gaba-transaminase a1r958 in complex with external aldimine plp-gaba adduct (see paper)
47% identity, 98% coverage: 4:422/429 of query aligns to 19:442/444 of 4atqF

query
sites
4atqF

K

K

N

S

A

Q

E

A

L

L

K

A

S

E

R

R

K

R

D

S

A

A

A

V

T

V

P

A

R

A

G

G

V
|
V

G

A

V

S

M

G

C

V

D

P

F

V

Y

Y

A

V

E

E

R

D

A

A

Q

D

N

G

A

G

E

I

L

I

W

R

D

D

V

V

E

D

G

G

R

N

R

S

F

F

I

I

D

D

F

L

A

G

A

S

G

G

I

I

A

A

V

V

C

T

N

S

T

V

G

G

H

A

R

S

H

D

P

P

K

A

I

V

V

V

A

A

I

V

R

Q

D

E

Q

A

L

A

D

A

R

H

F

F

T

T

H

H

T

T

A

C

Y

F

Q
x
M

I

V

V

T

P

P

Y

Y

A

E

S

G

Y

Y

V

V

E

A

L

V

A

T

E

E

K

Q

L

L

N

N

E

R

R

L

A

T

P

P

G

G

D

D

H

H

P

A

K

K

K

R

T

T

A

V

F

L

F

F

T

N

T

S

G
|
G

A
|
A

E

E

A

A

V

V

E

E

N

N

A

A

I

V

K

K

I

V

A

A

R

R

A

L

A

A

T

T

G

G

R

R

P

D

G

A

V

V

I

V

A

A

F

F

T

D

G

H

G

A

F
x
Y

H
|
H

G

G

R
|
R

T

T

L

N

M

L

G

T

M

M

A

A

L

L

T

T

G

A

K

K

V

A

A

M

P

P

Y

Y

K

K

A

T

G

N

F

F

G

G

P

P

F

F

P

A

S

P

D

E

V

V

F

Y

H

R

A

M

P

P

F

M

P

S

N

Y

P

P

L

F

H

R

-

E

-

N

-

P

G

E

V

I

S

T

T

G

A

A

D

E

S

A

L

A

K

K

A

R

I

A

E

I

F

T

L

M

F

I

K

E

A

K

D

Q

I

I

D

G

P

G

K

D

R

Q

V

V

A

A

I

I

F

I

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

F

Y

I

P

-

A

V

P

P

A

A

E

E

-

G

F

F

V

L

R

P

A

A

L

L

R

S

K

E

L

W

C

A

N

K

E

E

H

K

G

G

I

I

L

V

L

F

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

S

G

G

F

F

A

C

R

R

T

T

G

G

K

E

L

W

F

F

A

A

M

V

H

D

H

H

Y

E

D

G

V

V

P

P

D

D

L

I

M

I

T

T

V

M

A

A

K
|
K

S

G

L

I

A

A

G

G

M

L

P

P

L

L

S

S

G

A

V

I

I

T

G

G

R

R

A

A

D

D

V

L

M

L

D

D

A

A

A

V

A

H

P

P

G

G

L

L

G

G

G
|
G

T
|
T

Y

Y

A

G

G

G

N

N

P

P

L

V

A

A

V

C

A

A

L

L

A

A

V

A

L

I

D

D

I

T

I

M

D

E

E

Q

E

H

K

D

L

L

C

N

E

G

R

R

A

A

T

R

I

H

L

I

G

E

D

E

R

L

V

A

K

L

A

G

K

K

L

L

I

R

A

E

L

L

Q

A

N

A

E

E

A

G

P

G

Q

S

-

V

I

V

A

G

D

D

V

I

R

R

G

G

P

R

G

G

G

A

M

M

V

L

A

A

V

I

E

E

F

L

C

V

K

Q

P

P

G

G

S

S

T

K

E

E

P

P

D

N

A

A

E

E

F

L

T

T

K

K

R

A

V

V

Q

A

T

A

R

A

A

C

L

L

K

K

R

E

G

G

L

V

L

I

L

L

V

T

C

C

G

G

V

T

Y

Y

S

G

N

N

V

V

V

I

R
|
R

F

L

L

L

F

P

P

P

L

L

T

V

I

I

E

S

D

D

T

E

V

L

F

L

D

I

E

D

A

G

L

L

A

E

I

V

L

L

E

A

D

A

V

A

I

I

K

K

active site: V35 (= V20), Y154 (≠ F139), E226 (= E207), D259 (= D240), Q262 (= Q243), K288 (= K269), T317 (= T298), R418 (= R398)
binding gamma-amino-butanoic acid: M95 (≠ Q80), Y154 (≠ F139), R157 (= R142), E231 (= E212), K288 (= K269), G316 (= G297)
binding pyridoxal-5'-phosphate: G127 (= G112), A128 (= A113), Y154 (≠ F139), H155 (= H140), D259 (= D240), V261 (= V242)

6j2vA Gaba aminotransferase from corynebacterium glutamicum (see paper)
44% identity, 97% coverage: 4:417/429 of query aligns to 19:431/440 of 6j2vA

query
sites
6j2vA

K

K

N

S

A

K

E

A

L

L

K

D

S

E

R

R

K

R

D

Q

A

A

T

V

P

A

R

R

G

A

V
x
L

G

A

V

P

M

G

C

L

D

P

F

G

Y

Y

A

V

E

V

R

D

A

A

Q

D

N

G

A

G

E

I

L

L

W

A

D

D

V

A

E

D

G

G

R

N

R

R

F

F

I

I

D

D

F

L

A

A

A

S

G

G

I

I

A

A

V

V

C

T

N

T

T

V

G

G

H

G

R

S

H

N

P

A

K

A

I

V

V

A

A

K

A

A

I

V

R

G

D

A

Q

A

L

A

D

A

R

R

F

F

T

T

H

H

T

T

A

C

Y

F

Q

M

I

V

V

S

P

P

Y

Y

A

E

S

T

Y

Y

V

V

E

A

L

M

A

A

E

E

K

R

L

L

N

N

E

A

R

L

A

T

P

P

G

G

D

D

H

H

P

D

K

K

T

S

A

A

F

L

F

F

T

N

T

S

G
|
G

A
|
A

E

E

A

A

V

V

E

E

N

N

A

A

I

V

K

K

I

V

A

A

R

R

A

A

A

Y

T

T

G

G

R

K

P

G

G

A

V

V

I

V

A

V

F

F

T

D

G

N

G

A

F
x
Y

H
|
H

G

G

R
|
R

T

T

L

N

M

L

G

T

M

M

A

A

L

M

T

T

G

A

K

K

V

N

A

R

P

P

Y

Y

K

K

A

S

G

G

F

F

G

G

P

P

F

L

P

A

S

A

D

D

V

V

F

Y

H

R

A

A

P

P

F

M

P

S

N

Y

P

P

L

L

H

R

-

D

G

G

V

L

S

S

T

G

A

P

D

E

S

A

L

A

K

E

A

R

I

A

E

I

F

S

L

V

F

I

K

E

A

S

D

Q

I

V

D

G

P

A

K

E

R

N

V

L

A

A

A

C

I

V

F

I

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

F

-

I

Y

V

P

P

A

A

P

P

A

G

E

-

F

F

V

L

R

A

A

A

L

I

R

S

K

T

L

W

C

C

N

R

E

E

H

N

G

D

I

V

L

V

L

F

I

I

A

A

D
|
D

E

E

V
x
I

Q

Q

T

S

G

G

F

F

A

L

R

R

T

T

G

G

K

D

L

W

F

F

A

A

M

S

H

D

H

A

Y

E

D

G

V

V

V

I

P

P

D

D

L

V

M

I

T

T

V

T

A

A

K
|
K

S

G

L

I

A

A

G

G

M

M

P

P

L

L

S

S

G

A

V

V

I

T

G

G

R

R

A

A

D

E

V

I

M

M

D

D

A

A

A

P

A

G

P

P

G

G

G

A

L

L

G

G

G
|
G

T
|
T

Y

Y

A

G

G

G

N

N

P

P

L

V

A

A

V

C

A

A

L

L

A

A

V

A

L

I

D

E

I

V

I

M

D

E

E

Q

E

A

K

D

L

L

C

K

E

T

R

R

A

A

-

Q

-

E

-

I

-

E

T

T

I

I

L

I

G

R

D

D

R

E

V

F

K

-

A

A

K

Q

L

L

I

S

A

A

L

F

Q

-

N

-

E

-

A

-

P

P

Q

E

I

V

A

A

D

E

V

I

R

R

G

G

P

R

G

G

G

A

M

M

V

M

A

A

V

I

E

E

F

L

C

I

K

D

P

-

G

A

S

T

T

G

E

R

P

P

D

N

A

A

E

A

F

L

T

T

K

A

R

A

V

V

Q

A

T

A

R

R

A

A

L

K

K

A

R

E

G

G

L

V

L

L

V

T

C

C

G

G

V

T

Y

D

S

G

N

N

V

V

V

I

R

R

F

L

L

L

F

P

P

P

L

L

T

V

I

I

E

A

D

E

T

D

V

T

F

L

D

R

E

D

A

G

L

L

A

Q

I

V

L

L

active site: L35 (≠ V20), Y154 (≠ F139), D256 (= D240), K285 (= K269)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]butanoic acid: G127 (= G112), A128 (= A113), Y154 (≠ F139), H155 (= H140), R157 (= R142), E223 (= E207), E228 (= E212), D256 (= D240), I258 (≠ V242), K285 (= K269), G313 (= G297), T314 (= T298)

O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
37% identity, 98% coverage: 7:426/429 of query aligns to 34:463/474 of O58478

query
sites
O58478

E

E

L

L

K

I

S

E

R

R

K

E

D

K

A

K

A

V

T

L

P

S

R

T

G

G

V

I

G

G

V

V

-

K

M

L

C

F

D

P

F

L

Y

V

A

P

E

K

R

R

A

G

Q

F

N

G

A

P

E

F

L

I

W

E

D

D

V

V

E

D

G

G

R

N

R

V

F

F

I

I

D

D

F

F

A

L

A

A

G

G

I

A

A

A

V

A

C

A

N

S

T

T

G

G

H

Y

R

A

H

H

P

P

K

K

I

L

V

V

A

K

A

A

I

V

R

K

D

E

Q

Q

L

V

D

E

R

L

F

I

T

Q

H

H

T

S

A

M

Y

I

Q

G

I

Y

V

T

P

H

Y

S

A

E

S

R

Y

A

V

I

E

R

L

V

A

A

E

E

K

K

L

L

N

V

E

E

R

I

A

S

P

P

G

I

D

E

H

N

P

-

K

S

K

K

T

V

A

I

F

F

F

G

T

L

T

S

G

G

A

S

E

D

A

A

V

V

E

D

N

M

A

A

I

I

K

K

I

V

A

S

R

K

A

F

A

S

T

T

G

R

R

R

P

P

G

W

V

I

I

L

A

A

F

F

T

I

G

G

G

A

F

Y

H

H

G

G

R

Q

T

T

L

L

M

G

G

A

M

T

A

S

L

V

T

A

G

S

K

F

V

Q

A

V

P

S

Y

Q

K

K

A

R

G

G

F

Y

G

S

P

P

F

L

P

M

S

P

D

N

V

V

F

F

H

W

A

I

P

P

F

Y

P

P

N

N

P

P

-

F

-

R

-

N

-

I

-

W

-

G

L

I

H

N

G

G

V

Y

S

E

T

E

A

P

D

D

S

E

L

L

-

I

-

N

K

R

A

V

I

L

E

D

F

Y

L

L

-

E

-

Y

-

V

F

F

K

S

A

H

D

V

I

V

D

P

P

P

K

D

R

E

V

V

A

A

I

L

I

F

F

A

E

E

P

P

V

I

Q

Q

G

G

E
x
D

G

A

G

G

F

I

Y

V

P

V

A

P

P

P

A

E

E

N

F

F

V

F

R

K

A

E

L

L

R

K

K

K

L

L

C

L

N

E

E

E

H

Y

G

G

I

I

L

L

I

V

A

M

D

D

E

E

V

V

Q

Q

T

T

G

G

F

I

A

G

R

R

T

T

G

G

K

K

L

W

F

F

A

A

M

S

H

E

H

W

Y

F

D

N

V

V

V

K

P

P

D

D

L

M

M

I

T

I

V

F

A

G

K
|
K

S

G

L

V

A

A

G

S

G

G

M

M

P

G

L

L

S

S

G

G

V

V

I

I

G

G

R

R

A

K

D

E

V

I

M

M

D

D

A

I

A

T

A

S

P

G

G

S

G

A

L

L

G

-

G

L

T

T

Y

P

A

A

G

A

N

N

P

P

L

V

A

I

V

S

A

A

L

E

A

A

V

T

L

L

D

E

I

I

D

E

E

E

K

N

L

L

C

L

E

K

R

N

A

A

T

L

I

E

L

V

G

G

D

E

R

F

V

I

K

M

A

G

K

R

L

L

I

K

A

E

L

I

Q

K

N

E

E

R

A

F

P

E

Q

I

I

I

A

G

D

D

V

V

R

R

G

G

P

K

G

G

G

L

M

M

V

I

A

G

V

V

E

E

F

I

C

V

K

K

P

E

G

N

S

G

T

-

E

R

P

P

D

D

A

P

E

E

F

M

T

T

K

G

R

K

V

I

Q

C

T

W

R

R

A

A

L

F

K

E

R

L

G

G

L

L

L

I

L

L

L

P

V

S

C

Y

G

G

V

M

Y

F

S

G

N

N

V

V

V

I

R

R

F

I

L

T

F

P

P

P

L

L

T

V

I

L

E

T

D

K

T

E

V

V

F

A

D

E

E

K

A

A

L

L

A

E

I

I

L

I

E

E

D

R

V

A

I

I

K

K

D

D

S

T

V

L

A

T

D251 (≠ E212) mutation to A: Loss of activity.
K308 (= K269) mutation to A: Loss of activity.

5wyaA Structure of amino acid racemase, 2.65 a (see paper)
38% identity, 91% coverage: 29:419/429 of query aligns to 30:427/439 of 5wyaA

query
sites
5wyaA

E

D

R

H

A

A

Q

H

N

G

A

A

E

T

L

L

W

V

D

D

V

V

E

D

G

G

R

N

R

K

F

Y

I

I

D

D

F

L

A

L

A

A

G

S

I
x
A

A

S

V

A

C

I

N

N

T

V

G

G

H

H

R

T

H

H

P

E

K

K

I

V

V

V

A

K

A

A

I

I

R

A

D

D

Q

Q

L

A

D

Q

R

K

F

L

T

I

H

H

T

Y

A

T

Y

P

Q

A

I
x
Y

V

F

P

H

Y

H

A

V

S

P

Y

G

V

M

E

E

L

L

A

S

E

E

K

K

L

L

N

A

E

K

R

I

A

A

P

P

G

G

D

N

H

S

P

P

K

K

K

M

T

V

A

S

F

F

F

G

T

N

T
x
S

G
|
G

A
x
S

E

D

A

A

V

N

E

D

N

A

A

I

I

I

K

K

I

F

A

A

R

R

A

A

A

Y

T

T

G

G

R

R

P

Q

G

Y

V

I

I

V

A

S

F

Y

T

M

G

G

G

S

F
x
Y

H
|
H

G

G

R

S

T

T

L

Y

M

G

G

S

M

Q

A

T

L

L

T

S

G

G

K

S

V

S

A

L

P

N

Y

M

K

T

A

R

G

K

F

I

G

G

P

P

F

M

P

L

S

P

D

S

V

V

F

V

H

H

A

V

P

P

F

Y

P

P

N

D

P

S

L

Y

H

R

-

T

-

Y

-

P

G

G

V

E

S

T

T

E

A

H

D

D

-

V

S

S

L

L

K

R

A

Y

I

F

-

N

E

E

F

F

L

K

-

K

-

P

F

F

K

E

A

S

D

F

I

L

D

P

P

A

K

D

R

E

V

T

A

A

A

C

I

V

I

L

F

I

E
|
E

P

P

V

I

Q

Q

G

G

E

D

G

G

F

I

Y

I

P

K

A

A

P

P

A

E

E

E

F

Y

V

M

R

Q

A

L

L

V

R

Y

K

K

L

F

C

C

N

H

E

E

H

H

G

G

I

I

L

L

L

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

T

Q

G

G

F

L

A

G

R

R

T

T

G

G

K

K

L

M

F

W

A

A

M

I

H

Q

Y

F

-

K

D

D

V

I

V

E

P

P

D

D

L

L

M

M

T

S

V

V

A

G

K
|
K

S

S

L

L

A

A

G

S

G

G

M

M

P

P

L

L

S

S

G

A

V

V

I

I

G

G

R

K

A

K

D

E

V

V

M

M

D

Q

A

S

A

L

-

D

A

A

P

P

G

A

G

H

L

L

G
x
F

G
x
T

T

T

Y

-

A

A

G

G

N

N

P

P

L

V

A

C

V

S

A

A

A

S

L

L

A

A

V

T

L

L

D

D

I

V

I

I

D

E

E

Y

E

E

K

G

L

L

C

V

E

E

R

K

A

S

T

A

I

T

L

D

G

G

D

A

R

Y

V

A

K

K

A

Q

K

R

L

F

I

L

A

E

L

M

Q

Q

N

Q

E

R

A

H

P

P

Q

M

I

I

A

G

D

D

V

V

R

R

G

M

P

W

G

G

G

L

M

N

V

G

A

G

V

I

E

E

F

L

C

V

K

K

-

D

P

P

G

K

S

T

T

K

E

E

P

P

D

D

A

S

E

D

F

A

T

A

K

T

R

K

V

V

Q

I

T

Y

R

Y

A

A

L

F

K

A

R

H

G

G

L

V

L

I

V

T

C

L

G

A

V

-

Y

-

S

G

N

N

V

I

V

L

R
|
R

F

F

L

Q

F

P

P

P

L

L

T

V

I

I

E

P

D

R

T

E

V

Q

F

L

D

D

E

Q

A

A

L

L

A

Q

I

V

L

L

E

D

D

D

active site: Y140 (≠ F139), E215 (= E207), D248 (= D240), N251 (≠ Q243), K278 (= K269), T307 (≠ G297), R406 (= R398)
binding (2S,3S)-3-methyl-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]pentanoic acid: A52 (≠ I51), Y82 (≠ I81), S112 (≠ T111), G113 (= G112), S114 (≠ A113), Y140 (≠ F139), H141 (= H140), E215 (= E207), D248 (= D240), V250 (= V242), N251 (≠ Q243), K278 (= K269), F306 (≠ G296), T307 (≠ G297), R406 (= R398)

Sites not aligning to the query:

active site: 20

4ysnC Structure of aminoacid racemase in complex with plp (see paper)
38% identity, 91% coverage: 29:419/429 of query aligns to 39:436/448 of 4ysnC

query
sites
4ysnC

E

D

R

H

A

A

Q

H

N

G

A

A

E

T

L

L

W

V

D

D

V

V

E

D

G

G

R

N

R

K

F

Y

I

I

D

D

F

L

A

L

A

A

G

S

I

A

A

S

V

A

C

I

N

N

T

V

G

G

H

H

R

T

H

H

P

E

K

K

I

V

V

V

A

K

A

A

I

I

R

A

D

D

Q

Q

L

A

D

Q

R

K

F

L

T

I

H

H

T

Y

A

T

Y

P

Q

A

I

Y

V

F

P

H

Y

H

A

V

S

P

Y

G

V

M

E

E

L

L

A

S

E

E

K

K

L

L

N

A

E

K

R

I

A

A

P

P

G

G

D

N

H

S

P

P

K

K

K

M

T

V

A

S

F

F

F

G

T

N

T
x
S

G
|
G

A
x
S

E

D

A

A

V

N

E

D

N

A

A

I

I

I

K

K

I

F

A

A

R

R

A

A

A

Y

T

T

G

G

R

R

P

Q

G

Y

V

I

I

V

A

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F

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G

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F
x
Y

H
|
H

G

G

R

S

T

T

L

Y

M

G

G

S

M

Q

A

T

L

L

T

S

G

G

K

S

V

S

A

L

P

N

Y

M

K

T

A

R

G

K

F

I

G

G

P

P

F

M

P

L

S

P

D

S

V

V

F

V

H

H

A

V

P

P

F

Y

P

P

N

D

P

S

L

Y

H

R

-

T

-

Y

-

P

G

G

V

E

S

T

T

E

A

H

D

D

-

V

S

S

L

L

K

R

A

Y

I

F

-

N

E

E

F

F

L

K

-

K

-

P

F

F

K

E

A

S

D

F

I

L

D

P

P

A

K

D

R

E

V

T

A

A

A

C

I

V

I

L

F

I

E
|
E

P

P

V

I

Q

Q

G

G

E

D

G

G

F

I

Y

I

P

K

A

A

P

P

A

E

E

E

F

Y

V

M

R

Q

A

L

L

V

R

Y

K

K

L

F

C

C

N

H

E

E

H

H

G

G

I

I

L

L

L

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

T

Q

G

G

F

L

A

G

R

R

T

T

G

G

K

K

L

M

F

W

A

A

M

I

H

Q

Y

F

-

K

D

D

V

I

V

E

P

P

D

D

L

L

M

M

T

S

V

V

A

G

K
|
K

S

S

L

L

A

A

G

S

G

G

M

M

P

P

L

L

S

S

G

A

V

V

I

I

G

G

R

K

A

K

D

E

V

V

M

M

D

Q

A

S

A

L

-

D

A

A

P

P

G

A

G

H

L

L

G
x
F</