SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_03204 FitnessBrowser__Burk376:H281DRAFT_03204 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

Q8R2K1 Fucose mutarotase; EC 5.1.3.29 from Mus musculus (Mouse) (see paper)
38% identity, 94% coverage: 2:146/154 of query aligns to 4:148/153 of Q8R2K1

query
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Q8R2K1

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H24 (= H22) active site, Proton donor
D32 (= D30) binding
D69 (= D69) active site
M79 (= M79) binding
Y120 (= Y118) active site; binding
Y138 (= Y136) binding
N140 (≠ C138) binding

2wcuA Crystal structure of mammalian fucu (see paper)
38% identity, 94% coverage: 2:146/154 of query aligns to 4:148/149 of 2wcuA

query
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2wcuA

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binding alpha-L-fucopyranose: D32 (= D30), F35 (= F33), M79 (= M79), Y120 (= Y118), Y138 (= Y136), N140 (≠ C138)

4a34I Crystal structure of the fucose mutarotase in complex with l-fucose from streptococcus pneumoniae (see paper)
36% identity, 95% coverage: 2:147/154 of query aligns to 1:140/142 of 4a34I

query
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4a34I

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binding beta-L-fucopyranose: H21 (= H22), D29 (= D30), Y32 (≠ F33), Y65 (≠ F71), Y129 (= Y136), V138 (= V145)

2wcvB Crystal structure of bacterial fucu (see paper)
35% identity, 94% coverage: 1:145/154 of query aligns to 1:138/140 of 2wcvB

query
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2wcvB

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binding alpha-L-fucopyranose: D30 (= D30), F33 (= F33), M73 (≠ E80), Y111 (= Y118), K128 (≠ G135), Y129 (= Y136), N131 (≠ C138)

P0AEN8 L-fucose mutarotase; D-ribose pyranase; Fucose 1-epimerase; Type-2 mutarotase; EC 5.1.3.29; EC 5.4.99.62 from Escherichia coli (strain K12) (see paper)
35% identity, 94% coverage: 1:145/154 of query aligns to 1:138/140 of P0AEN8

query
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P0AEN8

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H22 (= H22) active site, Proton donor; mutation to A: Affects slightly binding affinity toward L-fucose. Significantly reduced ribose pyranase activity.
D30 (= D30) binding
D64 (= D69) active site; mutation to A: Affects slightly binding affinity toward L-fucose. Significantly reduced ribose pyranase activity.
M73 (≠ E80) binding
Y111 (= Y118) active site; binding ; mutation to F: Reduces binding affinity toward L-fucose. Significantly reduced ribose pyranase activity.
Y129 (= Y136) binding
N131 (≠ C138) binding

Query Sequence

>H281DRAFT_03204 FitnessBrowser__Burk376:H281DRAFT_03204
VLKNLDPLLNADILHALRSMGHGDELVICDANFPANSVARSSVLGKLLRLDGVSSPRAIR
AVLSVMPLDTFIDQPASRMEVVGEPHTMPAVQREAQAEVNAAEGRDVPFASIERFAFYER
ARKAYCVIATGEERGYGCFVFTKGVLLAPDAPRS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory