SitesBLAST
Comparing H281DRAFT_03219 FitnessBrowser__Burk376:H281DRAFT_03219 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 6 hits to proteins with known functional sites (download)
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
25% identity, 86% coverage: 22:391/429 of query aligns to 23:381/383 of 5i39A
- active site: F66 (≠ N66), Q69 (≠ F69), A70 (≠ V70), Q248 (vs. gap), P267 (≠ D269)
- binding flavin-adenine dinucleotide: V30 (= V29), G31 (= G30), G33 (= G32), I34 (≠ L33), L35 (≠ A34), V53 (≠ L53), E54 (= E54), K55 (≠ A55), Q62 (≠ A62), S63 (= S63), F66 (≠ N66), Y67 (≠ G67), Q69 (≠ F69), A196 (≠ P203), A197 (≠ V204), G226 (= G232), G227 (= G233), W229 (≠ Y235), Q248 (vs. gap), Q250 (vs. gap), G321 (= G334), M323 (= M336), T348 (vs. gap), G349 (= G361), W350 (≠ H362), G351 (= G363), M352 (= M364), T353 (≠ A365)
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
23% identity, 52% coverage: 168:391/429 of query aligns to 138:359/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (= Y283), R302 (≠ M336), R329 (≠ G361)
- binding flavin-adenine dinucleotide: V174 (= V204), S202 (≠ G232), G203 (= G233), W205 (≠ Y235), F209 (≠ V239), G300 (= G334), R302 (≠ M336), H327 (≠ F359), R329 (≠ G361), N330 (≠ H362), G331 (= G363), I332 (≠ M364)
- binding phosphate ion: R254 (= R291)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
- binding phosphate ion: 89
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
23% identity, 52% coverage: 168:391/429 of query aligns to 138:359/369 of O31616
- V174 (= V204) binding
- H244 (≠ R278) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (≠ M336) binding
- 327:333 (vs. 359:365, 14% identical) binding
- R329 (≠ G361) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
24% identity, 52% coverage: 168:391/429 of query aligns to 138:359/364 of 3if9A
- binding flavin-adenine dinucleotide: P173 (= P203), V174 (= V204), S202 (≠ G232), G203 (= G233), W205 (≠ Y235), F209 (≠ V239), G300 (= G334), R302 (≠ M336), H327 (≠ F359), F328 (≠ G360), R329 (≠ G361), N330 (≠ H362), G331 (= G363), I332 (≠ M364)
- binding glycolic acid: Y246 (= Y283), R302 (≠ M336), R329 (≠ G361)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49
5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
28% identity, 50% coverage: 22:237/429 of query aligns to 15:223/433 of 5hxwA
- active site: F58 (≠ N66), Q61 (≠ F69), A62 (≠ V70)
- binding flavin-adenine dinucleotide: V22 (= V29), G23 (= G30), G25 (= G32), I26 (≠ L33), L27 (≠ A34), E46 (= E54), K47 (≠ A55), E53 (≠ G61), Q54 (≠ A62), S55 (= S63), R57 (= R65), F58 (≠ N66), Y59 (≠ G67), G60 (= G68), Q61 (≠ F69), A188 (≠ P203), A189 (≠ V204), G218 (= G232), G219 (= G233), W221 (≠ Y235)
Sites not aligning to the query:
- active site: 240, 284, 288
- binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
- binding flavin-adenine dinucleotide: 240, 242, 331, 371, 373, 398, 399, 400, 401, 402, 403
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
22% identity, 80% coverage: 48:391/429 of query aligns to 28:359/369 of S5FMM4
- G51 (≠ F71) mutation to S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- A54 (≠ Y74) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- K81 (≠ V101) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
- S202 (≠ G232) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ M364) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ L374) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Query Sequence
>H281DRAFT_03219 FitnessBrowser__Burk376:H281DRAFT_03219
MESFYEATVTRPSAYAPLVGQHSADVCIVGGGLAGLSTALGLVERGVKNVAVLEARQVGY
GASGRNGGFVFGGYSLDCADLLKTLGASRARELYALTTDAVDLMRARIARYRIDCDVTDA
GVILANWFDEPARLEAQRRLMLESFGVDWEPVAAEHLARQLKTRRYHGGLYERNAFHFHP
LKYVLGVAQAAVSAGVRIHEQSPVVHLQRDGAGFVLRTPQGVIDARHVVMAGGGYARNVY
AKVERAVLPIATYVMATEPLGARLHDAIDTRAAVYDTRFAFDYYRPLPDTRILWGGRISV
RDRAPEVIARLLRRDLLKVYPQLHDVRVDYAWGGLMSYARHKMPQIGRTADGVWHAVGFG
GHGMAPTTVSGELLAAAIAGERAVPEAFAAFGLTPAYGALGLAAAQLTYTAMQTRDVFAA
RRTRARHSV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory