SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_03298 FitnessBrowser__Burk376:H281DRAFT_03298 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q46444 Quinohemoprotein alcohol dehydrogenase; QH-ADH; Alcohol dehydrogenase (azurin); PQQ-containing alcohol dehydrogenase; PQQ-dependent ADH; Quinohaemoprotein ethanol dehydrogenase type I; QH-EDHI; EC 1.1.9.1 from Comamonas testosteroni (Pseudomonas testosteroni) (see 3 papers)
25% identity, 55% coverage: 383:866/879 of query aligns to 161:577/708 of Q46444

query
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Q46444

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T198 (= T430) binding
GA 214:215 (≠ VT 446:447) binding
E216 (≠ D448) binding
T274 (≠ N496) binding
N294 (= N516) binding
D339 (= D560) binding
K366 (= K587) binding
NW 425:426 (≠ DW 684:685) binding
V575 (≠ S864) binding

Sites not aligning to the query:

1:31 signal peptide
101 binding
147 modified: Disulfide link with 148
148 modified: Disulfide link with 147
153 binding
635 binding covalent
638 binding covalent
639 binding axial binding residue
678 binding axial binding residue

1kb0A Crystal structure of quinohemoprotein alcohol dehydrogenase from comamonas testosteroni (see paper)
25% identity, 55% coverage: 383:866/879 of query aligns to 130:546/670 of 1kb0A

query
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1kb0A

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active site: E185 (≠ T452), N263 (= N516), D308 (= D560)
binding calcium ion: E185 (≠ T452), N263 (= N516), D308 (= D560)
binding pyrroloquinoline quinone: T167 (= T430), G182 (≠ N449), G183 (≠ E450), A184 (≠ S451), E185 (≠ T452), T243 (≠ N496), W245 (= W498), D308 (= D560), K335 (= K587), N394 (≠ D684), W395 (= W685), W479 (≠ S797), G543 (= G863), V544 (≠ S864)
binding tetrahydrofuran-2-carboxylic acid: E185 (≠ T452), D308 (= D560), P389 (≠ N679)

Sites not aligning to the query:

binding heme c: 598, 599, 602, 603, 617, 620, 631, 637, 640, 642, 643, 645
binding pyrroloquinoline quinone: 70, 116, 117, 122
binding tetrahydrofuran-2-carboxylic acid: 116, 117

O05542 Alcohol dehydrogenase (quinone), dehydrogenase subunit; ADH; Alcohol dehydrogenase (quinone), acceptor subunit; Alcohol dehydrogenase (quinone), subunit I; Ethanol:Q2 reductase; G3-ADH subunit I; Quinohemoprotein alcohol dehydrogenase; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
35% identity, 14% coverage: 160:285/879 of query aligns to 40:157/757 of O05542

query
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O05542

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Sites not aligning to the query:

1:34 signal peptide
35 modified: Pyrrolidone carboxylic acid

8gy2A Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
35% identity, 14% coverage: 160:285/879 of query aligns to 6:123/723 of 8gy2A

query
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8gy2A

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T

T

P

P

I

L

K

I

V

V

G

N

N

G

K

V

L

M

F

Y

L

A

C

T

T

T

P

N

H

W

S

S

I

K

V

M

I

K

A

A

L

L

D

D

P

A

A

A

S

T

G

G

K

K

E

L

L

L

W

W

R

S

Y

Y

D

D

P

P

H

K

I

V

Q

P

S

G

P

N

V

I

G

A

F
x
D

K

R

H

G

W
x
C

E
x
C

H

D

M

T

T

V

C

S

R

R

G

G

V

A

S

A

Y

Y

H

W

D

N

D

G

A

K

M

V

Y

Y

binding heme c: D104 (≠ F266)
binding pyrroloquinoline quinone: C107 (≠ W269), C108 (≠ E270)

Sites not aligning to the query:

binding calcium ion: 181, 263, 308
binding heme c: 618, 619, 622, 623, 633, 634, 636, 639, 652, 660, 662, 665
binding pyrroloquinoline quinone: 163, 179, 180, 181, 245, 263, 308, 335, 398, 489

2d0vA Crystal structure of methanol dehydrogenase from hyphomicrobium denitrificans (see paper)
23% identity, 55% coverage: 379:860/879 of query aligns to 119:536/597 of 2d0vA

query
sites
2d0vA

E

K

C

T

P

P

R

S

R

L

I

I

F

I

L

K

P

T

T

Q

A

L

D

D

A

G

R

H

L

L

I

V

A

A

L

L

N

N

A

A

D

K

T

T

G

G

Q

E

P

E

C

F

T

W

H

K

F

V

G

-

N

E

N

N

G

G

Q

D

I

I

D

K

L

V

R

G

T

Q

N

T

I

L

G

-

P

-

F

-

T

-

P

-

G

-

Y

-

S

-

T
|
T

S

Q

P

A

P

P

A

Y

V

V

T

V

R

H

D

D

L

L

V

A

I

I

I

V

S

G

-

S

-
x
S

-
x
G

-
x
A

-
x
E

-

L

-

G

-

V

-

R

G

G

H

H

V

V

T

T

D

-

N

-

E

-

S

-

T

-

N

-

E

-

P

-

S

-

G

-

V

-

T

-

R

-

A

A

F

Y

D

N

V

V

H

R

D

T

G

G

H

E

L

Q

V

A

W

W

-

R

-

Y

-

A

-

T

-

G

-

P

-

D

-

A

-

E

-

I

-

G

-

L

-

A

-

D

N

D

W

F

D

N

A

S

G

A

N

N

P

P

D

H

E

Y

T

G

Q

Q

P

K

I

G

T

L

G

G

N

T

Q

A

T

T

Y

W

V

E

R

G

N

D

S

A

P

W

N

K

M

I

-

G

-
x
T

-

N

W
|
W

S

G

V

W

F

Y

S

A

V

Y

D

D

E

P

K

A

L

A

G

N

M

L

V

I

Y

Y

L

Y

P

G

L

S

G

G

N
|
N

Q

P

T

A

P

P

D

-

Q

-

W

W

G

N

G

E

M

T

R

M

T

R

P

P

A

G

S

D

E

N

K

K

V

W

A

T

A

M

G

T

V

I

V

T

A

A

L

R

D

D

L

A

A

D

T

T

G

G

K

K

M

M

R

K

W

F

N

G

Y

Y

Q

Q

F

K

T

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

F

D

-

V

A

G

G

G

V

Q

N

P

V

S

I

L

M

I

L

D

S

L

E

Q

Q

T

T

-

D

P

K

S

T

G

G

V

K

Q

K

P

R

A

K

L

L

I

L

A

T

S

H

-

P

T

D

K
x
R

Q

N

G

G

S

I

L

V

Y

Y

V

T

L

L

N

D

R

R

E

E

T

N

G

G

K

D

P

L

I

I

V

-

P

-

I

-

T

-

E

-

P

-

V

-

P

-

Q

-

G

-

A

-

G

-

T

S

G

A

D

D

H

K

T

L

S

D

P

D

T

T

-

V

Q

N

P

V

F

F

S

K

A

T

L

V

N

D

F

L

K

K

P

T

P

G

K

L

V

P

R

V

E

R

R

D

D

P

M

E

W

Y

G

G

T

T

N

-

P

-

F

-

D

-

Q

-

L

-

W

-

C

-

R

-

V

-

K

-

F

-

K

-

S

-

L

-

R

R

Y

M

D

D

G

-

M

-

F

-

T

-

P

-

S

-

E

H

Q

K

G

G

S

T

L

D

V

I

F

C

P

P

G

S

N

A

F

M

G

G

V

Y

F

H

D
x
N

W

Q

G

G

G

H

I

D

A

S

V

Y

D

D

P

P

V

Q

R

K

Q

Q

I

L

L

F

I

F

A

M

N

G

P

I

S

N

Y

H

M

I

A

C

F

M

T

D

S

W

K

E

L

P

V

F

P

-

R

-

S

-

Q

M

I

L

P

P

S

Y

D

R

N

A

G

G

E

Q

K

-

G

-

S

-

E

-

T

-

S

-

G

-

I

-

K

-

L

-

A

-

R

-

G

-

T

F

P

F

Y

V

G

G

F

A

E

T

L

L

N

W

A

M

F

Y

L

P

S

G

P

P

L

K

G

G

I

D

P

R

C

Q

Q

N

A

Y

P

L

P

G

W

L

G

G

Y

Q

V

I

A

K

G

A

V

Y

D

N

L

A

R

I

T

T

N

N

H

E

I

Y

V

K

W

W

E

Q

H

H

K

-

N

-

G

-

T

-

I

-

R

-

D

-

S

-

A

-

P

-

L

-

P

-

I

-

P

-

M

-

P

-

L

M

G

E

V

R

P

F

S

S

L

V

G
x
W

G

G

M

G

I

T

T

L

T

A

A

T

G

A

G

G

V

N

A

L

F

V

L

F

S

Y

G

G

T

T

L

L

D

D

Y

G

Y

F

L

L

R

K

A

A

Y

R

D

N

V

S

R

D

T

T

G

G

D

E

R

L

L

V

W

W

Q

K

A

H

R

K

L

L

P

P

A

S

G

G

G

V

Q

I

A

G

T

Y

P

P

M

M

T

T

Y

Y

A

-

D

E

S

H

N

K

G

G

K

V

Q

Q

Y

Y

V

I

L

A

V

V

T

M

A

S

G

G

active site: E177 (vs. gap), N261 (= N516), D303 (= D560)
binding calcium ion: E177 (vs. gap), N261 (= N516), D303 (= D560)
binding pyrroloquinoline quinone: T159 (= T430), S174 (vs. gap), G175 (vs. gap), A176 (vs. gap), E177 (vs. gap), T241 (vs. gap), W243 (= W498), R331 (≠ K587), N394 (≠ D684), W476 (≠ G799)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 55, 109, 539, 540

5xm3A Crystal structure of methanol dehydrogenase from methylophaga aminisulfidivorans (see paper)
27% identity, 28% coverage: 357:602/879 of query aligns to 97:346/596 of 5xm3A

query
sites
5xm3A

A

A

T

R

A

A

A

V

S

A

D
x
C

T

C

S

D

A

V

T

N

Q
x
R

Q

G

T

L

A

A

A

Y

A

W

A

P

V

G

S

D

A

D

E

K

C

A

P

P

R

A

R

M

I

I

F

V

L

K

P

S

T

L

A

L

D

D

A

G

R

H

L

V

I

V

A

A

L

L

N

N

A

A

D

E

T

T

G

G

Q

E

P

E

C

Y

T

W

H

K

F

V

G

-

N

E

N

N

G

G

Q

D

I

I

D

S

L

V

R

-

T

-

N

-

I

-

G

-

P

-

F

-

T

-

P

-

G

-

G

G

Y

Q

Y

T

S

E

T
|
T

S

A

P

A

P

P

A

F

V

V

T

A

R

K

D

D

L

L

V

V

I

I

-

Q

-

G

-

S

-
x
S

-
x
G

-
x
A

-
x
E

-

L

-

G

I

V

S

R

G

G

H

Y

V

V

T

T

D

-

N

-

E

-

S

-

T

-

N

-

E

-

P

-

S

-

G

-

V

-

T

-

R

-

A

A

F

Y

D

D

V

I

H

H

D

T

G

G

H

E

L

M

V

V

W

W

N

R

W

W

-

Y

-

A

-

T

-

G

-

P

-

D

-

A

D

D

A

V

G

G

-

L

-

D

-

K

-

D

-

F

-

N

-

K

-

H

N

N

P

P

D

H

E

Y

T

G

Q

Q

P

K

I

G

T

L

G

G

N

T

Q

S

T

T

Y

W

V

E

R

D

N

N

S

A

P

W

N

K

M

I

-

G

-
x
T

-

N

W
|
W

S

G

V

W

F

Y

S

A

V

Y

D

D

E

P

K

Q

L

L

G

D

M

M

V

F

Y

Y

L

Y

P

G

L

S

G

G

N
|
N

Q

P

T

A

P

P

D

-

Q

-

W

W

G

N

G

E

M

T

R

M

T

R

P

P

A

G

S

D

E

N

K

K

V

W

A

T

A

M

G

T

V

I

V

W

A

G

L

R

D

D

L

L

A

E

T

T

G

G

K

L

M

A

R

K

W

F

N

G

Y

Y

Q

Q

F

K

T

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

Y

D

A

V

G

G

V

G

N

Q

V

P

M

S

M

L

L

I

S

D

E

L

Q

Q

K

T

D

P

K

S

N

G

G

V

K

Q

M

P

R

A

K

L

L

I

L

A

T

S

H

-

P

T

D

K
x
R

Q

N

G

G

S

I

L

I

Y

Y

V

T

L

L

N

D

R

R

E

E

T

T

G

G

K

D

P

L

I

V

active site: E177 (vs. gap), N261 (= N516), D303 (= D560)
binding magnesium ion: E177 (vs. gap), N261 (= N516)
binding pyrroloquinoline quinone: C103 (≠ D363), R109 (≠ Q369), T159 (= T430), S174 (vs. gap), G175 (vs. gap), A176 (vs. gap), E177 (vs. gap), T241 (vs. gap), W243 (= W498), R331 (≠ K587)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 55, 394, 476, 539, 540

7ce5A Methanol-pqq bound methanol dehydrogenase (mdh) from methylococcus capsulatus (bath) (see paper)
25% identity, 28% coverage: 357:599/879 of query aligns to 97:342/573 of 7ce5A

query
sites
7ce5A

A

A

T

R

A

A

A

V

S

A

D
x
C

T
x
C

S

D

A

V

T

N

Q
x
R

Q

G

T

L

A

A

A

Y

A

V

A

P

V

A

S

G

A

E

E

H

C

G

P

P

R

A

R

K

I

I

F

F

L

L

P

N

T

Q

A

L

D

D

A

G

R

H

L

I

I

V

A

A

L

L

N

N

A

A

D

K

T

T

G

G

Q

E

P

E

C

I

T

W

H

K

F

M

G

-

N

E

N

N

G

S

Q

D

I

I

D

A

L

M

R

G

T

S

N

T

I

L

G

-

P

-

F

-

T

-

P

-

G

-

Y

-

S

-

T
|
T

S

G

P

A

P

P

A

F

V

V

T

V

R

K

D

D

L

K

V

V

I

L

I

V

S

G

G

S

H
x
A

V
x
G

T
x
A

D
x
E

N

L

E

G

S

V

T

-

N

-

E

-

P

-

S

R

G

G

V

Y

T

V

R

T

A

A

F

Y

D

N

V

I

H

K

D

D

G

G

H

K

L

Q

V

E

W

W

N

R

W

A

D

Y

A

A

G

T

N

G

P

P

D

D

E

E

T

D

Q

L

P

L

I

L

T

-

G

-

N

D

Q

K

T

D

Y

F

V

N

R

K

N

D

S

N

P

P

N

H

M

Y

-

G

-

Q

-

F

-

G

-

L

-

G

-

L

-

S

-

T

-

W

-

E

-

G

-

D

-

A

-

W

-

K

-

I

-

G

-
x
T

-

N

W
|
W

S

G

V

W

F

Y

S

A

V

Y

D

D

E

P

K

K

L

L

G

D

M

M

V

I

Y

Y

L

Y

P

G

L

S

G

G

N
|
N

Q

P

T

A

P

P

D

-

Q

-

W

W

G

N

G

E

M

T

R

M

T

R

P

P

A

G

S

D

E

N

K

K

V

W

A

T

A

M

G

T

V

I

V

W

A

G

L

R

D

D

L

A

A

D

T

T

G

G

K

R

M

A

R

K

W

F

N

G

Y

Y

Q

Q

F

K

T

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

Y

D

A

V

G

G

V

G

N

Q

Y

P

M

S

G

L

L

I

S

D

E

L

Q

Q

E

T

V

P

D

S

G

G

K

V

L

Q

T

P

P

A

L

L

L

I

T

A

H

S

P

T

D

K
x
R

Q

N

G

G

S

L

L

V

Y

Y

V

T

L

L

N

N

R

R

E

E

T

T

G

G

active site: E177 (≠ D448), N261 (= N516), D303 (= D560)
binding calcium ion: E177 (≠ D448), N261 (= N516), D303 (= D560)
binding methanol: E177 (≠ D448)
binding pyrroloquinoline quinone: C103 (≠ D363), C104 (≠ T364), R109 (≠ Q369), T159 (= T430), A174 (≠ H445), G175 (≠ V446), A176 (≠ T447), E177 (≠ D448), T241 (vs. gap), W243 (= W498), R330 (≠ K587)

Sites not aligning to the query:

7cdlC Holo-methanol dehydrogenase (mdh) with cys131-cys132 reduced from methylococcus capsulatus (bath) (see paper)
25% identity, 28% coverage: 357:599/879 of query aligns to 97:342/573 of 7cdlC

query
sites
7cdlC

A

A

T

R

A

A

A

V

S

A

D

C

T

C

S

D

A

V

T

N

Q
x
R

Q

G

T

L

A

A

A

Y

A

V

A

P

V

A

S

G

A

E

E

H

C

G

P

P

R

A

R

K

I

I

F

F

L

L

P

N

T

Q

A

L

D

D

A

G

R

H

L

I

I

V

A

A

L

L

N

N

A

A

D

K

T

T

G

G

Q

E

P

E

C

I

T

W

H

K

F

M

G

-

N

E

N

N

G

S

Q

D

I

I

D

A

L

M

R

G

T

S

N

T

I

L

G

-

P

-

F

-

T

-

P

-

G

-

Y

-

S

-

T
|
T

S

G

P

A

P

P

A

F

V

V

T

V

R

K

D

D

L

K

V

V

I

L

I

V

S

G

G

S

H
x
A

V

G

T
x
A

D
x
E

N

L

E

G

S

V

T

-

N

-

E

-

P

-

S

R

G

G

V

Y

T

V

R

T

A

A

F

Y

D

N

V

I

H

K

D

D

G

G

H

K

L

Q

V

E

W

W

N

R

W

A

D

Y

A

A

G

T

N

G

P

P

D

D

E

E

T

D

Q

L

P

L

I

L

T

-

G

-

N

D

Q

K

T

D

Y

F

V

N

R

K

N

D

S

N

P

P

N

H

M

Y

-

G

-

Q

-

F

-

G

-

L

-

G

-

L

-

S

-

T

-

W

-

E

-

G

-

D

-

A

-

W

-

K

-

I

-

G

-
x
T

-

N

W
|
W

S

G

V

W

F

Y

S

A

V

Y

D

D

E

P

K

K

L

L

G

D

M

M

V

I

Y

Y

L

Y

P

G

L

S

G

G

N
|
N

Q

P

T

A

P

P

D

-

Q

-

W

W

G

N

G

E

M

T

R

M

T

R

P

P

A

G

S

D

E

N

K

K

V

W

A

T

A

M

G

T

V

I

V

W

A

G

L

R

D

D

L

A

A

D

T

T

G

G

K

R

M

A

R

K

W

F

N

G

Y

Y

Q

Q

F

K

T

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

Y

D

A

V

G

G

V

G

N

Q

Y

P

M

S

G

L

L

I

S

D

E

L

Q

Q

E

T

V

P

D

S

G

G

K

V

L

Q

T

P

P

A

L

L

L

I

T

A

H

S

P

T

D

K
x
R

Q

N

G

G

S

L

L

V

Y

Y

V

T

L

L

N

N

R

R

E

E

T

T

G

G

active site: E177 (≠ D448), N261 (= N516), D303 (= D560)
binding calcium ion: E177 (≠ D448), N261 (= N516), D303 (= D560)
binding pyrroloquinoline quinone: R109 (≠ Q369), T159 (= T430), A174 (≠ H445), A176 (≠ T447), E177 (≠ D448), T241 (vs. gap), W243 (= W498), D303 (= D560), R330 (≠ K587)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 55, 393, 469, 532, 533

Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
23% identity, 56% coverage: 383:870/879 of query aligns to 152:577/718 of Q4W6G0

query
sites
Q4W6G0

R
x
K

I
x
V

F
x
Y

L
x
V

P
x
G

T
x
V

A
x
L

D
|
D

A
x
G

R
|
R

L
|
L

I
x
E

A
|
A

L
x
I

N
x
D

A
|
A

D
x
K

T
|
T

G
|
G

Q
|
Q

P
x
R

C
x
A

T
x
W

H

-

F

-

G

-

N

-

G

-

Q
x
S

I
x
V

D
|
D

L
x
T

R
|
R

T
x
A

N
x
D

I

-

G

-

P

-

F

-

T
x
H

P
x
K

G
x
R

G
x
S

Y
|
Y

Y
x
T

S
x
I

T
|
T

S
x
G

P
x
A

P
|
P

A
x
R

V
|
V

T
x
V

R
x
N

D
x
G

L
x
K

V
|
V

I
x
V

I
|
I

S
x
G

G

-

H

-

V

-

T

-

D
x
N

N
x
G

E
x
G

S
x
A

T
x
E

N
x
F

E
x
G

P
x
V

S
x
R

G
|
G

V
x
Y

T
x
V

R
x
T

A
|
A

F
x
Y

D
|
D

V
x
A

H
x
E

D
x
T

G
|
G

H
x
K

L
x
E

V
x
A

W
|
W

N
x
R

W
x
F

D
x
Y

A
x
T

G
x
V

N
x
P

P
x
G

D
|
D

E
x
P

T
x
K

Q
x
L

P
|
P

I
x
P

T
x
E

G
|
G

N
x
K

-
x
G

-
x
M

-
x
E

-
x
I

-
x
A

-
x
K

-
x
T

-
x
W

-
x
F

-
x
G

Q
x
D

T
x
A

Y
|
Y

V
|
V

R
x
E

N
x
Q

S
x
G

P
x
G

-
x
G

N
x
T

M
x
A

W
|
W

S
x
D

V
x
S

F
|
F

S
x
A

V
x
Y

D
|
D

E
x
P

K
x
E

L
|
L

G
x
N

M
x
L

V
x
L

Y
|
Y

L
x
I

P
x
G

L
x
V

G
|
G

N
|
N

Q

-

T

-

P

-

D

-

Q

-

W

-

G
|
G

G
x
S

M
x
L

R
x
W

T
x
D

P
|
P

A
x
K

S
x
W

E
x
R

K
x
S

V
x
Q

A
|
A

A
x
K

G
|
G

-
x
D

-
x
N

-
x
L

-
x
F

-
x
L

-
x
S

V
x
I

V
|
V

A
|
A

L
x
V

D
x
N

L
x
A

A
x
D

T
|
T

G
|
G

K
x
E

M
x
Y

R
x
V

W
|
W

N
x
H

Y
|
Y

Q
|
Q

F
x
T

T
|
T

H
x
P

H
x
G

D
|
D

L
x
A

W
|
W

D
|
D

M
x
Y

D
x
T

V
x
A

G
x
T

G
x
Q

Q
x
H

P
x
M

S
x
I

L
|
L

I
x
A

D
x
E

L
|
L

Q
x
P

T
x
I

P
x
D

S
x
G

G
x
K

V
x
P

Q
x
R

P
x
K

A
x
V

L
|
L

I
x
M

A
x
Q

S
x
A

T
x
P

K
|
K

Q
x
N

G
|
G

S
x
F

L
x
F

Y
|
Y

V
|
V

L
x
I

N
x
D

R
|
R

E
x
A

T
|
T

G
|
G

K
x
E

P
x
L

I
x
L

V
x
S

P

-

I

-

T
x
A

E
x
K

E
x
G

P
x
I

V
|
V

P
|
P

Q
|
Q

G

-

A

-

G

-

T

-

G

-

D

-

H

-

T

-

S
|
S

P
x
W

T
|
T

Q
x
K

P
x
G

F
x
M

S
x
D

A
x
M

L
x
K

N
x
T

F
x
G

K
x
R

P
|
P

P
x
I

K
x
L

V
x
D

R
x
E

E
|
E

R
x
N

D
x
A

M
x
A

W
x
Y

G
x
W

T
x
K

N
|
N

P

-

F

-

D

-

Q

-

L

-

W

-

C

-

R

-

V

-

K

-

F

-

K

-

S

-

L

-

R

-

Y

-

D

-

G

-

M

-

F

-

T

-

P

-

S

-

E
x
G

Q
x
K

G
x
R

S
x
N

L
|
L

V
|
V

F
x
T

P
|
P

G
x
A

N
x
F

F
x
W

G
|
G

V
x
A

F
x
H

D
|
D

W
|
W

G
x
Q

G
x
P

I
x
M

A
x
S

V
x
Y

D
x
N

P
|
P

V
x
D

R
x
T

Q
x
G

I
x
L

L
x
V

I
x
Y

A
x
I

N
x
P

P
x
A

S
x
H

Y
x
I

M
|
M

A
x
S

F
x
A

T
x
Y

S
x
Y

K
x
E

L
x
H

V
x
I

P
|
P

R
x
E

S
x
A

Q

-

I

-

P
|
P

S
x
K

D
x
R

N
|
N

G
x
P

E
x
F

K
|
K

K
x
S

G
x
M

S
x
Y

E
x
Q

T
x
L

S

-

G
|
G

I
x
L

K
x
R

L
x
T

A
x
G

R
x
M

G

-

T
x
M

P
|
P

Y
x
E

G
|
G

F
x
A

E
|
E

L

-

N

-

A

-

F

-

L

-

S

-

P

-

L

-

G
|
G

I
x
L

P
x
L

C
x
E

Q
x
M

A
|
A

P
x
K

P
x
S

W
|
W

-
x
S

G
|
G

Y
x
K

V
x
L

A
x
I

G
x
A

V
x
W

D
|
D

L
x
P

R
x
V

T
x
K

N
x
Q

H
x
Q

I
x
A

V
x
A

W
|
W

E
|
E

H
x
V

K
x
P

N
x
Y

G
x
V

T
|
T

I
|
I

R
x
F

D
x
N

S

-

A

-

P

-

L

-

P

-

I

-

P

-

M

-

P

-

L

-

G

-

V

-

P

-

S

-

L

-

G
|
G

M
x
T

I
x
L

T
x
S

T
|
T

A
|
A

G
|
G

G
x
N

V
x
L

A
x
V

F
|
F

L

-

S
x
E

G
|
G

T
x
S

L
x
A

D
|
D

Y
x
G

Y
x
R

L
x
V

R
x
I

A
|
A

Y
|
Y

D
x
A

V
x
A

R
x
D

T
|
T

G
|
G

D
x
E

R
x
K

L
|
L

W
|
W

Q
x
E

A
x
Q

R
x
P

L
x
A

P
x
A

A
x
S

G
|
G

G
x
V

Q
x
M

A
|
A

T
x
A

P
|
P

M
x
V

T
|
T

Y
|
Y

A
x
S

D

-

S
x
V

N
x
D

G
|
G

K
x
E

Q
|
Q

Y
|
Y

V
|
V

L
x
T

V
x
F

T
x
M

A
|
A

G
|
G

G
x
W

H
x
G

G
|
G

S
x
A

L
x
F

G
x
S

T
|
T

K
x
F

Q
x
A

G
|
G

T189 (= T430) binding
GA 205:206 (≠ ES 450:451) binding
E207 (≠ T452) binding
T264 (≠ N496) binding
N284 (= N516) binding
D329 (= D560) binding
K356 (= K587) binding
W415 (≠ F680) binding
DW 419:420 (= DW 684:685) binding

Sites not aligning to the query:

1:29 signal peptide
30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
138 modified: Disulfide link with 139
139 modified: Disulfide link with 138
144 binding
635 binding covalent
638 binding covalent
639 binding axial binding residue
676 binding axial binding residue

1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
23% identity, 56% coverage: 383:870/879 of query aligns to 123:548/684 of 1yiqA

query
sites
1yiqA

R

K

I

V

F

Y

L

V

P

G

T

V

A

L

D

D

A

G

R

R

L

L

I

E

A

A

L

I

N

D

A

A

D

K

T

T

G

G

Q

Q

P

R

C

A

T

W

H

-

F

-

G

-

N

-

G

-

Q

S

I

V

D

D

L

T

R

R

T

A

N

D

I

-

G

-

P

-

F

-

T

H

P

K

G

R

G

S

Y

Y

Y

T

S

I

T
|
T

S

G

P

A

P

P

A

R

V

V

T

V

R

N

D

G

L

K

V

V

I

V

I

I

S

G

G

-

H

-

V

-

T

-

D

N

N
x
G

E
x
G

S
x
A

T
x
E

N

F

E

G

P

V

S

R

G

G

V

Y

T

V

R

T

A

A

F

Y

D

D

V

A

H

E

D

T

G

G

H

K

L

E

V

A

W

W

N

R

W

F

D

Y

A

T

G

V

N

P

P

G

D

D

E

P

T

K

Q

L

P

P

I

P

T

E

G

G

N

K

-

G

-

M

-

E

-

I

-

A

-

K

-

T

-

W

-

F

-

G

Q

D

T

A

Y

Y

V

V

R

E

N

Q

S

G

P

G

-

G

N
x
T

M

A

W
|
W

S

D

V

S

F

F

S

A

V

Y

D

D

E

P

K

E

L

L

G

N

M

L

V

L

Y

Y

L

I

P

G

L

V

G

G

N
|
N

Q

-

T

-

P

-

D

-

Q

-

W

-

G

G

G

S

M

L

R

W

T

D

P

P

A

K

S

W

E

R

K

S

V

Q

A

A

A

K

G

G

-

D

-

N

-

L

-

F

-

L

-

S

V

I

V

V

A

A

L

V

D

N

L

A

A

D

T

T

G

G

K

E

M

Y

R

V

W

W

N

H

Y

Y

Q

Q

F

T

T

T

H

P

H

G

D

D

L

A

W

W

D
|
D

M

Y

D

T

V

A

G

T

G

Q

Q

H

P

M

S

I

L

L

I

A

D

E

L

L

Q

P

T

I

P

D

S

G

G

K

V

P

Q

R

P

K

A

V

L

L

I

M

A

Q

S

A

T

P

K
|
K

Q

N

G

G

S

F

L

F

Y

Y

V

V

L

I

N

D

R

R

E

A

T

T

G

G

K

E

P

L

I

L

V

S

P

-

I

-

T

A

E

K

E

G

P

I

V

V

P

P

Q

Q

G

-

A

-

G

-

T

-

G

-

D

-

H

-

T

-

S

S

P

W

T

T

Q

K

P

G

F

M

S

D

A

M

L

K

N

T

F

G

K

R

P

P

P

I

K

L

V

D

R

E

E

E

R

N

D

A

M

A

W

Y

G

W

T

K

N

N

P

G

F

-

D

-

Q

-

L

-

W

-

C

-

R

-

V

-

K

-

F

-

K

-

S

-

L

-

R

-

Y

-

D

-

G

-

M

-

F

-

T

-

P

-

S

-

E

-

Q

K

G

R

S

N

L

L

V

V

F

T

P

P

G

A

N

F

F

W

G

G

V

A

F

H

D
|
D

W
|
W

G

Q

G

P

I

M

A

S

V

Y

D

N

P

P

V

D

R

T

Q

G

I

L

L

V

I

Y

A

I

N

P

P

A

S

H

Y

I

M

M

A

S

F

A

T

Y

S

Y

K

E

L

H

V

I

P

P

R

E

S

A

Q

-

I

-

P

P

S

K

D

R

N

N

G

P

E

F

K

K

K

S

G

M

S

Y

E

Q

T

L

S

-

G

G

I

L

K

R

L

T

A

G

R

M

G

-

T

M

P

P

Y

E

G

G

F

A

E

E

L

-

N

-

A

-

F

-

L

-

S

-

P

-

L

-

G

G

I

L

P

L

C

E

Q

M

A

A

P

K

P

S

W

W

-

S

G

G

Y

K

V

L

A

I

G

A

V

W

D

D

L

P

R

V

T

K

N

Q

H

Q

I

A

V

A

W

W

E

E

H

V

K

P

N

Y

G

V

T

T

I

I

R
x
F

D

N

S

-

A

-

P

-

L

-

P

-

I

-

P

-

M

-

P

-

L

-

G

-

V

-

P

-

S

-

L

-

G

G

M

T

I

L

T

S

T

T

A

A

G

G

G

N

V

L

A

V

F

F

L

-

S

E

G

G

T

S

L

A

D

D

Y

G

Y

R

L

V

R

I

A

A

Y

Y

D

A

V

A

R

D

T

T

G

G

D

E

R

K

L

L

W

W

Q

E

A

Q

R

P

L

A

P

A

A

S

G

G

G

V

Q

M

A

A

T

A

P

P

M

V

T

T

Y

Y

A

S

D

-

S

V

N

D

G

G

K

E

Q

Q

Y

Y

V

V

L

T

V

F

T

M

A

A

G

G

G

W

H

G

G

G

S
x
A

L

F

G

S

T

T

K

F

Q

A

G

G

active site: E178 (≠ T452), N255 (= N516), D300 (= D560)
binding calcium ion: E178 (≠ T452), N255 (= N516), D300 (= D560)
binding pyrroloquinoline quinone: T160 (= T430), G175 (≠ N449), G176 (≠ E450), A177 (≠ S451), E178 (≠ T452), T235 (≠ N496), W237 (= W498), K327 (= K587), D390 (= D684), W391 (= W685), F477 (≠ R782), A542 (≠ S864)

Sites not aligning to the query:

binding heme c: 605, 606, 608, 609, 610, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650
binding pyrroloquinoline quinone: 63, 109, 110, 115

6damA Crystal structure of lanthanide-dependent methanol dehydrogenase xoxf from methylomicrobium buryatense 5g (see paper)
26% identity, 25% coverage: 383:603/879 of query aligns to 117:344/563 of 6damA

query
sites
6damA

R

K

I

I

F

F

L

L

P

Q

T

Q

A

S

D

D

A

T

R

V

L

L

I

T

A

A

L

L

N

D

A

A

D

K

T

T

G

G

Q

K

P

R

C

V

T

W

H

S

F

V

G

-

N

Q

N

N

G

G

Q

D

I

P

D

K

L

L

R

-

T

-

N

-

I

-

G

-

P

-

F

-

T

-

P

-

G

-

G

G

Y

M

Y

T

S

N

T
|
T

S

Q

P

A

P

P

A

L

V

V

T

V

R

K

D

D

L

K

V

V

I

I

-

T

-

G

I

I

S
|
S

G
|
G

H

-

V

-

T

-

D

-

N

-

E

-

S
x
G

T
x
E

N

F

E

G

P

V

S

R

G

G

V

F

T

L

R

A

A

A

F

Y

D

N

V

I

H

R

D

T

G

G

H

E

L

L

V

D

W

W

N

K

W

G

D

Y

A

S

G

M

N

G

P

P

D

D

-

A

-

D

-

T

-

L

-

I

-

N

-

P

-

T

-

K

-

T

-

W

-

K

-

D

-

G

E

K

T

V

Q

Q

P

P

I

V

T

G

G

K

N

D

Q

S

T

S

Y

T

V

S

R

T

N

W

S

E

P

G

N

D

M

Q

W

W

S

K

V

I

-

G

-
x
T

-

T

-
x
W

-

G

-

W

F

Y

S

S

V

Y

D

D

E

P

K

E

L

L

G

N

M

L

V

V

Y

Y

L

Y

P

G

L

S

G

G

N
|
N

Q

-

T

-

P

P

D

S

Q

T

W

W

G

N

G

P

M

A

R

Q

T

R

P

P

A

G

S

D

E

N

K

K

V

W

A

S

A

M

G

S

V

L

V

W

A

A

L

R

D

N

L

A

A

D

T

T

G

G

K

E

M

V

R

K

W

W

N

V

Y

Y

Q

Q

F

M

T

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

Y

D
|
D

V

G

G

I

G

N

Q

E

P

V

S

A

L

L

I

V

D

D

L

Q

Q

E

T

I

P

K

S

G

G

Q

V

M

Q

R

P

K

A

T

L

A

I

V

A

H

S

F

T

D

K
x
R

Q

N

G

G

S

F

L

G

Y

Y

V

T

L

L

N

D

R

R

E

V

T

T

G

G

K

E

P

L

I

L

V

V

active site: E171 (≠ T452), N259 (= N516), D301 (= D560)
binding pyrroloquinoline quinone: T153 (= T430), S168 (= S443), G169 (= G444), G170 (≠ S451), E171 (≠ T452), T239 (vs. gap), W241 (vs. gap), D303 (= D562), R328 (≠ K587)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 55, 103, 104, 109, 394, 480, 543, 544

4aahA Methanol dehydrogenase from methylophilus w3a1 (see paper)
27% identity, 26% coverage: 383:609/879 of query aligns to 117:346/571 of 4aahA

query
sites
4aahA

R

Q

I

I

F

V

L

K

P

K

T

Q

A

A

D

N

A

G

R

H

L

L

I

L

A

A

L

L

N

D

A

A

D

K

T

T

G

G

Q

K

P

I

C

-

T

-

H

-

F

-

G

-

N

-

N

N

G

W

Q

E

I

V

D

E

L

V

R

-

T

-

N

-

I

C

G

D

P

P

F

K

T

V

P

-

G

-

G

G

Y

S

Y

T

S

L

T

T

S

Q

P

A

P

P

A

F

V

V

T

A

R

K

D

D

L

T

V

V

I

L

I

M

S

G

G

C

H

-

V

-

T

-

D

-

N
x
S

E

G

S
x
A

T
x
E

N

L

E

G

P

V

S

R

G

G

V

A

T

V

R

N

A

A

F

F

D

D

V

L

H

K

D

T

G

G

H

E

L

L

V

K

W

W

-

R

-

A

-

F

-

A

-

T

-

G

-

S

-

D

-

S

-

V

-

R

-

L

-

A

-

K

N

D

W

F

D

N

A

S

G

A

N

N

P

P

D

H

E

Y

T

G

Q

Q

P

F

I

G

T

L

G

G

N

T

Q

K

T

T

Y

W

V

E

R

G

N

D

S

A

P

W

N

K

M

I

-

G

-

T

-

N

W
|
W

S

G

V

W

F

Y

S

A

V

Y

D

D

E

P

K

K

L

L

G

N

M

L

V

F

Y

Y

L

Y

P

G

L

S

G

G

N
|
N

Q

P

T

A

P

P

D

-

Q

-

W

W

G

N

G

E

M

T

R

M

T

R

P

P

A

G

S

D

E

N

K

K

V

W

A

T

A

M

G

T

V

I

V

W

A

G

L

R

D

D

L

L

A

D

T

T

G

G

K

M

M

A

R

K

W

W

N

G

Y

Y

Q

Q

F

K

T

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

F

D

A

V

G

G

V

G

N

Q

Q

P

M

S

V

L

L

I

T

D

D

L

Q

Q

P

T

V

P

N

S

G

G

K

V

M

Q

T

P

P

A

L

L

L

I

S

A

H

S

I

T

D

K
x
R

Q

N

G

G

S

I

L

L

Y

Y

V

T

L

L

N

N

R

R

E

E

T

N

G

G

K

N

P

L

I

I

V

V

P

A

I

E

T

K

E

V

E

D

P

P

active site: E171 (≠ T452), N255 (= N516), D297 (= D560)
binding calcium ion: E171 (≠ T452), N255 (= N516)
binding pyrroloquinoline quinone: S168 (≠ N449), A170 (≠ S451), E171 (≠ T452), W237 (= W498), R324 (≠ K587)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 55, 103, 104, 109, 387, 467, 531

1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
27% identity, 25% coverage: 383:602/879 of query aligns to 119:337/664 of 1kv9A

query
sites
1kv9A

R

K

I

V

F

Y

L

V

P

G

T

T

A

L

D

D

A

G

R

R

L

L

I

I

A

A

L

L

N

D

A

A

D

K

T

T

G

G

Q

K

P

A

C

-

T

-

H

-

F

-

G

-

N

-

G

-

Q

-

I

I

D

W

L

S

R

Q

T

Q

N

T

I

T

G

D

P

P

F

A

T

K

P

P

G

-

Y

Y

Y

S

S

I

T
|
T

S

G

P

A

P

P

A

R

V

V

T

V

R

K

D

G

L

K

V

V

I

I

S

G

G

-

H

-

V

-

T

-

D

N

N
x
G

E

G

S
x
A

T
x
E

N

Y

E

G

P

V

S

R

G

G

V

F

T

V

R

S

A

A

F

Y

D

D

V

A

H

D

D

T

G

G

H

K

L

L

V

A

W

W

N

R

W

F

D

Y

A

T

-

V

-

P

G

G

N

D

P

P

-

A

-

L

-

P

-

Y

-

E

-

H

-

P

-

E

-

L

-

R

D

E

E

A

T

A

Q

K

P

T

I

W

T

Q

G

G

N

D

Q

Q

T

Y

Y

W

-

K

V

L

R

G

N

G

S

G

P

G

N
x
T

M

V

W
|
W

S

D

V

S

F

M

S

A

V

Y

D

D

E

P

K

E

L

L

G

D

M

L

V

L

Y

Y

L

V

P

G

L

T

G

G

N
|
N

Q

G

T

S

P

P

D

-

Q

-

W

W

G

N

-

R

G

E

M

V

R

R

T

S

P

P

A

G

-

G

S

D

E

N

K

L

V

Y

A

L

A

S

G

S

V

I

V

L

A

A

L

I

D

R

L

P

A

D

T

T

G

G

K

K

M

L

R

A

W

W

N

H

Y

Y

Q

Q

F

V

T

T

H

P

H

G

D

D

L

S

W

W

D
|
D

M

F

D

T

V

A

G

T

G

Q

Q

Q

P

I

S

T

L

L

I

A

D

E

L

L

Q

N

T

I

P

D

S

G

G

K

V

P

Q

R

P

K

A

V

L

L

I

M

A

Q

S

A

T

P

K
|
K

Q

N

G

G

S

F

L

F

Y

Y

V

V

L

L

N

D

R

R

E

T

T

N

G

G

K

K

P

L

I

I

active site: E173 (≠ T452), N250 (= N516), D295 (= D560)
binding acetone: E173 (≠ T452), D295 (= D560)
binding calcium ion: E173 (≠ T452), N250 (= N516), D295 (= D560)
binding pyrroloquinoline quinone: T155 (= T430), G170 (≠ N449), A172 (≠ S451), E173 (≠ T452), T230 (≠ N496), W232 (= W498), K322 (= K587)

Sites not aligning to the query:

binding heme c: 101, 102, 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 623, 631, 633, 636
binding pyrroloquinoline quinone: 59, 105, 106, 111, 382, 383, 460, 525

Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
27% identity, 25% coverage: 383:602/879 of query aligns to 141:359/690 of Q8GR64

query
sites
Q8GR64

R
x
K

I
x
V

F
x
Y

L
x
V

P
x
G

T
|
T

A
x
L

D
|
D

A
x
G

R
|
R

L
|
L

I
|
I

A
|
A

L
|
L

N
x
D

A
|
A

D
x
K

T
|
T

G
|
G

Q
x
K

P
x
A

C

-

T

-

H

-

F

-

G

-

N

-

G

-

Q

-

I
|
I

D
x
W

L
x
S

R
x
Q

T
x
Q

N
x
T

I
x
T

G
x
D

P
|
P

F
x
A

T
x
K

P
|
P

G

-

Y
|
Y

Y
x
S

S
x
I

T
|
T

S
x
G

P
x
A

P
|
P

A
x
R

V
|
V

T
x
V

R
x
K

D
x
G

L
x
K

V
|
V

I
|
I

S
x
G

G

-

H

-

V

-

T

-

D
x
N

N
x
G

E
x
G

S
x
A

T
x
E

N
x
Y

E
x
G

P
x
V

S
x
R

G
|
G

V
x
F

T
x
V

R
x
S

A
|
A

F
x
Y

D
|
D

V
x
A

H
x
D

D
x
T

G
|
G

H
x
K

L
|
L

V
x
A

W
|
W

N
x
R

W
x
F

D
x
Y

A
x
T

-
x
V

-
x
P

G
|
G

N
x
D

P
|
P

-
x
A

-
x
L

-
x
P

-
x
Y

-
x
E

-
x
H

-
x
P

-
x
E

-
x
L

-
x
R

D
x
E

E
x
A

T
x
A

Q
x
K

P
x
T

I
x
W

T
x
Q

G
|
G

N
x
D

Q
|
Q

T
x
Y

Y
x
W

-
x
K

V
x
L

R
x
G

N
x
G

S
x
G

P
x
G

N
x
T

M
x
V

W
|
W

S
x
D

V
x
S

F
x
M

S
x
A

V
x
Y

D
|
D

E
x
P

K
x
E

L
|
L

G
x
D

M
x
L

V
x
L

Y
|
Y

L
x
V

P
x
G

L
x
T

G
|
G

N
|
N

Q
x
G

T
x
S

P
|
P

D

-

Q

-

W
|
W

G
x
N

-
x
R

G
x
E

M
x
V

R
|
R

T
x
S

P
|
P

A
x
G

-
x
G

S
x
D

E
x
N

K
x
L

V
x
Y

A
x
L

A
x
S

G
x
S

V
x
I

V
x
L

A
|
A

L
x
I

D
x
R

L
x
P

A
x
D

T
|
T

G
|
G

K
|
K

M
x
L

R
x
A

W
|
W

N
x
H

Y
|
Y

Q
|
Q

F
x
V

T
|
T

H
x
P

H
x
G

D
|
D

L
x
S

W
|
W

D
|
D

M
x
F

D
x
T

V
x
A

G
x
T

G
x
Q

Q
|
Q

P
x
I

S
x
T

L
|
L

I
x
A

D
x
E

L
|
L

Q
x
N

T
x
I

P
x
D

S
x
G

G
x
K

V
x
P

Q
x
R

P
x
K

A
x
V

L
|
L

I
x
M

A
x
Q

S
x
A

T
x
P

K
|
K

Q
x
N

G
|
G

S
x
F

L
x
F

Y
|
Y

V
|
V

L
|
L

N
x
D

R
|
R

E
x
T

T
x
N

G
|
G

K
|
K

P
x
L

I
|
I

T177 (= T430) binding
GA 193:194 (≠ ES 450:451) binding
E195 (≠ T452) binding
T252 (≠ N496) binding
N272 (= N516) binding
D317 (= D560) binding
K344 (= K587) binding

Sites not aligning to the query:

1:22 signal peptide
23:690 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH IIB
81 binding
127 modified: Disulfide link with 128
128 modified: Disulfide link with 127
133 binding
404:405 binding
547 binding
613 binding covalent
616 binding covalent
617 binding axial binding residue
655 binding axial binding residue

4maeA Methanol dehydrogenase from methylacidiphilum fumariolicum solv (see paper)
26% identity, 29% coverage: 383:640/879 of query aligns to 118:360/577 of 4maeA

query
sites
4maeA

R

K

I

I

F

F

L

M

P

T

T

T

A

L

D

D

A

G

R

Q

L

I

I

I

A

A

L

L

N

D

A

A

D

N

T

T

G

G

Q

K

P

-

C

-

T

-

H

-

F

-

G

-

N

-

G

-

Q

-

I

-

D

E

L

L

R

W

T

K

N

M

I

K

G

H

P

A

F

D

T

V

P

T

G

K

G

G

Y

E

Y

T

S

I

T
|
T

S

G

P

A

P

P

A

L

V

V

T

V

R

K

D

D

L

K

V

V

I

L

I

V

S

G

G

-

H

-

V

V

T
x
S

D
x
G

N
x
G

E
|
E

S

F

T

G

N

-

E

-

P

V

S

R

G

G

V

R

T

V

R

G

A

A

F

Y

D

D

V

I

H

N

D

T

G

G

H

N

L

R

V

V

W

W

N

L

W

A

D

Y

A

S

G

Q

N

G

P

P

D

D

E

E

T

E

-

V

-

L

-

D

-

S

-

D

-

F

-

N

-

K

-

E

-

F

-

P

-

Q

-

H

-

G

Q

G

P

P

I

G

T

D

G

G

N

T

Q

K

T

T

Y

W

V

P

R

G

N

E

S

Q

P

W

N

K

M

L

-

G

-
x
T

-

T

W
|
W

S

G

V

W

F

Y

S

S

V

Y

D

D

E

P

K

A

L

L

G

D

M

L

V

F

Y

Y

L

Y

P

-

L

-

G

G

N

T

Q

S

T
x
N

P

P

D

G

Q

T

W

W

G

N

G

A

-

E

M

Q

R

R

T

K

P

G

A

G

S

D

E

N

K

K

V

W

A

S

A

C

G

T

V

I

V

F

A

A

L

R

D

R

L

P

A

D

T

T

G

G

K

K

M

A

R

R

W

W

N

A

Y

Y

Q

Q

F

M

T

T

H

P

H

W

D

D

L

S

W

W

D
|
D

M

Y

D
|
D

V

G

G

V

G

N

Q

E

P

M

S

I

L

L

I

P

D

D

L

L

Q

T

T

V

P

K

S

G

G

K

V

K

Q

T

P

P

A

C

L

L

I

V

A

H

S

F

T

D

K
x
R

Q

N

G

G

S

F

L

G

Y

Y

V

V

L

L

N

D

R

R

E

R

T

T

G

G

K

Q

P

L

I

I

V

-

P

-

I

-

T

-

E

-

P

-

V

-

P

-

Q

-

G

-

A

-

G

-

T

-

G

-

D

-

H

-

T

-

S

-

P

E

T

A

Q

Q

P

P

F

F

S

V

A

Y

L

V

N

N

F

W

K

A

P

K

P

E

K

I

V

S

R

K

E

E

R

N

D

D

active site: E172 (= E450), N256 (≠ T518), D299 (= D560)
binding cerium (iii) ion: E172 (= E450), N256 (≠ T518), D299 (= D560), D301 (= D562)
binding pyrroloquinoline quinone: T154 (= T430), S169 (≠ T447), G170 (≠ D448), G171 (≠ N449), E172 (= E450), T236 (vs. gap), W238 (= W498), D301 (= D562), R326 (≠ K587)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 55, 104, 105, 110, 388, 467, 531, 532

6fkwA Europium-containing methanol dehydrogenase (see paper)
26% identity, 29% coverage: 383:640/879 of query aligns to 118:360/576 of 6fkwA

query
sites
6fkwA

R

K

I

I

F

F

L

M

P

T

T

T

A

L

D

D

A

G

R

Q

L

I

I

I

A

A

L

L

N

D

A

A

D

N

T

T

G

G

Q

K

P

-

C

-

T

-

H

-

F

-

G

-

N

-

G

-

Q

-

I

-

D

E

L

L

R

W

T

K

N

M

I

K

G

H

P

A

F

D

T

V

P

T

G

K

G

G

Y

E

Y

T

S

I

T
|
T

S

G

P

A

P

P

A

L

V

V

T

V

R

K

D

D

L

K

V

V

I

L

I

V

S

G

G

-

H

-

V

V

T
x
S

D
x
G

N
x
G

E
|
E

S

F

T

G

N

-

E

-

P

V

S

R

G

G

V

R

T

V

R

G

A

A

F

Y

D

D

V

I

H

N

D

T

G

G

H

N

L

R

V

V

W

W

N

L

W

A

D

Y

A

S

G

Q

N

G

P

P

D

D

E

E

T

E

-

V

-

L

-

D

-

S

-

D

-

F

-

N

-

K

-

E

-

F

-

P

-

Q

-

H

-

G

Q

G

P

P

I

G

T

D

G

G

N

T

Q

K

T

T

Y

W

V

P

R

G

N

E

S

Q

P

W

N

K

M

L

-

G

-
x
T

-

T

W
|
W

S

G

V

W

F

Y

S

S

V

Y

D

D

E

P

K

A

L

L

G

D

M

L

V

F

Y

Y

L

Y

P

-

L

-

G

G

N

T

Q

S

T
x
N

P

P

D

G

Q

T

W

W

G

N

G

A

-

E

M

Q

R

R

T

K

P

G

A

G

S

D

E

N

K

K

V

W

A

S

A

C

G

T

V

I

V

F

A

A

L

R

D

R

L

P

A

D

T

T

G

G

K

K

M

A

R

R

W

W

N

A

Y

Y

Q

Q

F

M

T

T

H

P

H

W

D

D

L

S

W

W

D
|
D

M

Y

D
|
D

V

G

G

V

G

N

Q

E

P

M

S

I

L

L

I

P

D

D

L

L

Q

T

T

V

P

K

S

G

G

K

V

K

Q

T

P

P

A

C

L

L

I

V

A

H

S

F

T

D

K
x
R

Q

N

G

G

S

F

L

G

Y

Y

V

V

L

L

N

D

R

R

E

R

T

T

G

G

K

Q

P

L

I

I

V

-

P

-

I

-

T

-

E

-

P

-

V

-

P

-

Q

-

G

-

A

-

G

-

T

-

G

-

D

-

H

-

T

-

S

-

P

E

T

A

Q

Q

P

P

F

F

S

V

A

Y

L

V

N

N

F

W

K

A

P

K

P

E

K

I

V

S

R

K

E

E

R

N

D

D

active site: E172 (= E450), N256 (≠ T518), D299 (= D560), D301 (= D562)
binding europium ion: E172 (= E450), N256 (≠ T518), D299 (= D560), D301 (= D562)
binding pyrroloquinoline quinone: T154 (= T430), S169 (≠ T447), G170 (≠ D448), G171 (≠ N449), E172 (= E450), T236 (vs. gap), W238 (= W498), D301 (= D562), R326 (≠ K587)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 55, 104, 105, 110, 388, 467, 531, 532

6oc6A Lanthanide-dependent methanol dehydrogenase xoxf from methylobacterium extorquens, in complex with lanthanum and pyrroloquinoline quinone (see paper)
26% identity, 26% coverage: 384:610/879 of query aligns to 118:347/579 of 6oc6A

query
sites
6oc6A

I

I

F

L

L

L

P

H

T

Q

A

A

D

D

A

T

R

T

L

L

I

V

A

S

L

L

N

D

A

A

D

K

T

S

G

G

Q

K

P

V

C

N

T

W

H

S

F

V

G

K

N

N

N

G

G

D

Q

P

I

S

D

K

L

G

R

E

T

T

N

N

I
x
T

G

-

P

-

F

-

T

-

P

-

G

-

Y

-

S

A

T

T

S

V

P

L

P

P

A

V

V

K

T

D

R

K

D

V

L

I

V

V

I

G

I

I

S
|
S

G
|
G

-
x
G

-
x
E

-

F

-

G

-

V

-

Q

-

C

H

H

V

V

T

T

D

-

N

-

E

-

S

-

T

-

N

-

E

-

P

-

S

-

G

-

V

-

T

-

R

-

A

A

F

Y

D

D

V

L

H

K

D

S

G

G

H

K

L

K

V

V

W

W

N

R

W

G

D

Y

A

S

G

I

N

G

P

P

D

D

E

D

T

Q

Q

L

P

I

I

V

T

D

G

P

N

E

Q

K

T

T

Y

T

V

S

R

L

N

G

S

K

P

P

-

I

-

G

-

K

-

D

-

S

-

L

-

K

-

T

-

W

-

E

-

G

-

D

-

Q

-

W

-

K

-

T

-

G

N

C

M

T

W
|
W

S

G

V

W

F

F

S

S

V

Y

D

D

E

P

K

K

L

L

G

D

M

L

V

M

Y

Y

L

Y

P

G

L

S

G

G

N
|
N

Q

-

T

-

P

P

D

S

Q

T

W

W

G

N

G

P

M

K

R

Q

T

R

P

P

A

G

S

D

E

N

K

K

V

W

A

S

A

M

G

T

V

I

V

W

A

A

L

R

D

N

L

P

A

D

T

T

G

G

K

M

M

A

R

K

W

W

N

V

Y

Y

Q

Q

F

M

T

T

H

P

H

H

D

D

L

E

W

W

D
|
D

M

F

D
|
D

V

G

G

I

G

N

Q

E

P

M

S

I

L

L

I

T

D

D

L

Q

Q

K

T

F

P

D

S

G

G

K

V

D

Q

R

P

P

A

L

L

L

I

T

A

H

S

F

T

D

K
x
R

Q

N

G

G

S

F

L

G

Y

Y

V

T

L

L

N

D

R

R

E

A

T

T

G

G

K

E

P

V

I

L

V

V

P

A

I

E

T

K

E

F

E

D

P

P

V

V

active site: E171 (vs. gap), N255 (= N516), D297 (= D560)
binding pyrroloquinoline quinone: T153 (≠ I419), S168 (= S443), G169 (= G444), G170 (vs. gap), E171 (vs. gap), W237 (= W498), D299 (= D562), R324 (≠ K587)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 55, 103, 104, 109, 395, 473, 536, 537

7o6zB Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
26% identity, 25% coverage: 383:602/879 of query aligns to 119:356/588 of 7o6zB

query
sites
7o6zB

R

K

I

I

F

V

L

F

P

N

T

T

A

L

D

D

A

G

R

Y

L

V

I

V

A

C

L

L

N

D

A

A

D

N

T

T

G

G

Q

K

P

E

C

L

-

W

-

K

T

T

H

K

F

F

G

A

N

D

N

V

G

N

Q

K

I

-

D

-

L

-

R

-

T

-

N

-

I

-

G

-

P

-

F

-

T

-

P

-

G

-

G

G

Y

E

Y

T

S

S

T
|
T

S

P

P

A

P

P

A

I

V

I

T

V

R

K

D

D

L

K

V

V

I

V

I

-

S

T

G

G

H

Y

V
x
G

T
x
G

D
|
D

N
x
E

E

F

S

G

T

A

N

-

E

-

P

-

S

R

G

G

V

R

T

F

R

A

A

A

F

F

D

D

V

L

H

N

D

S

G

G

H

K

L

M

V

V

W

W

N

Q

W

A

D

Y

A

S

G

N

N

G

P

P

D

D

E

S

-

D

-

V

-

L

-

G

-

P

-

D

-

F

-

N

-

S

T

K

Q

H

P

P

I

E

T

Y

G

G

-

Q

-

A

-

G

-

Q

-

D

-

L

-

G

-

V

-

K

-

T

-

Y

-

P

N

D

Q

E

T

E

Y

W

V

K

R

R

N

G

S

G

P

G

N

C

M

A

W
|
W

S

G

V

W

F

Y

S

S

V

Y

D

D

E

P

K

K

L

L

G

D

M

L

V

I

Y

Y

L

Y

P

N

L

T

G

G

N
|
N

Q

-

T

-

P

P

D

G

Q

L

W
|
W

G

S

-

P

G

S

M

Y

R

R

T

T

P

E

A

A

S

K

-

T

-

H

-

E

-

A

-

N

-

E

-

P

-

W

-

K

-

W

-

D

E

N

K

K

V

W

A

S

A

M

G

T

V

I

V

F

A

A

L

R

D

K

L

P

A

D

T

T

G

G

K

E

M

A

R

V

W

W

N

G

Y

Y

Q

Q

F

M

T

T

H

P

H

F

D

D

L

Q

W

W

D
|
D

M

Y

D
|
D

V

G

G

I

G

N

Q

E

P

D

S

V

L

L

I

V

D

D

L

I

Q

T

T

V

P

D

S

G

G

S

V

K

Q

K

P

P

A

C

L

L

I

V

A

H

S

F

T

D

K
x
R

Q

N

G

G

S

F

L

C

Y

Y

V

V

L

L

N

N

R

R

E

T

T

D

G

G

K

T

P

I

I

I

binding methanol: E173 (≠ N449), W263 (= W522), D314 (= D560)
binding Neodymium Ion: E173 (≠ N449), N259 (= N516), D314 (= D560), D316 (= D562)
binding pyrroloquinoline quinone: T155 (= T430), G170 (≠ V446), G171 (≠ T447), D172 (= D448), E173 (≠ N449), W241 (= W498), D316 (= D562), R341 (≠ K587)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 55, 105, 106, 111, 403, 481, 544, 545

7o6zA Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
26% identity, 25% coverage: 383:602/879 of query aligns to 119:356/588 of 7o6zA

query
sites
7o6zA

R

K

I

I

F

V

L

F

P

N

T

T

A

L

D

D

A

G

R

Y

L

V

I

V

A

C

L

L

N

D

A

A

D

N

T

T

G

G

Q

K

P

E

C

L

-

W

-

K

T

T

H

K

F

F

G

A

N

D

N

V

G

N

Q

K

I

-

D

-

L

-

R

-

T

-

N

-

I

-

G

-

P

-

F

-

T

-

P

-

G

-

G

G

Y

E

Y

T

S

S

T

T

S

P

P

A

P

P

A

I

V

I

T

V

R

K

D

D

L

K

V

V

I

V

I

-

S

T

G

G

H

Y

V

G

T

G

D

D

N
x
E

E

F

S

G

T

A

N

-

E

-

P

-

S

R

G

G

V

R

T

F

R

A

A

A

F

F

D

D

V

L

H

N

D

S

G

G

H

K

L

M

V

V

W

W

N

Q

W

A

D

Y

A

S

G

N

N

G

P

P

D

D

E

S

-

D

-

V

-

L

-

G

-

P

-

D

-

F

-

N

-

S

T

K

Q

H

P

P

I

E

T

Y

G

G

-

Q

-

A

-

G

-

Q

-

D

-

L

-

G

-

V

-

K

-

T

-

Y

-

P

N

D

Q

E

T

E

Y

W

V

K

R

R

N

G

S

G

P

G

N

C

M

A

W

W

S

G

V

W

F

Y

S

S

V

Y

D

D

E

P

K

K

L

L

G

D

M

L

V

I

Y

Y

L

Y

P

N

L

T

G

G

N
|
N

Q

-

T

-

P

P

D

G

Q

L

W
|
W

G

S

-

P

G

S

M

Y

R

R

T

T

P

E

A

A

S

K

-

T

-

H

-

E

-

A

-

N

-

E

-

P

-

W

-

K

-

W

-

D

E

N

K

K

V

W

A

S

A

M

G

T

V

I

V

F

A

A

L

R

D

K

L

P

A

D

T

T

G

G

K

E

M

A

R

V

W

W

N

G

Y

Y

Q

Q

F

M

T

T

H

P

H

F

D

D

L

Q

W

W

D
|
D

M

Y

D
|
D

V

G

G

I

G

N

Q

E

P

D

S

V

L

L

I

V

D

D

L

I

Q

T

T

V

P

D

S

G

G

S

V

K

Q

K

P

P

A

C

L

L

I

V

A

H

S

F

T

D

K

R

Q

N

G

G

S

F

L

C

Y

Y

V

V

L

L

N

N

R

R

E

T

T

D

G

G

K

T

P

I

I

I

binding methanol: E173 (≠ N449), W263 (= W522), D314 (= D560)
binding Neodymium Ion: E173 (≠ N449), N259 (= N516), D314 (= D560), D316 (= D562)

Sites not aligning to the query:

binding methanol: 545

6zcvA Crystal structure of lanthanide-dependent alcohol dehydrogenase pedh from pseudomonas putida kt2440
25% identity, 25% coverage: 384:602/879 of query aligns to 119:338/562 of 6zcvA

query
sites
6zcvA

I

V

F

F

L

F

P

G

T

T

A

L

D

D

A

A

R

K

L

L

I

V

A

A

L

L

N

N

A

K

D

D

T

T

G

G

Q

K

P

-

C

-

T

-

H

-

F

-

G

-

N

-

G

-

Q

-

I

V

D

V

L

W

R

S

T

K

N

K

I

V

G

A

P

D

F

H

T

K

P

E

G

G

G

Y

Y

S

Y

I

S
|
S

T

A

S

A

P

P

P

-

A

-

V

-

T

-

R

-

D

-

L

-

V

M

I

I

I

V

S
x
N

G

G

H
x
K

V

L

T

I

D

T

N

G

E

V

S

A

T
x
G

N
x
G

E
|
E

P

F

S

G

-

V

G

G

V

K

T

I

R

Q

A

A

F

Y

D

N

V

P

H

E

D

N

G

G

H

E

L

L

V

L

W

W

N

M

-

R

-

P

-

T

-

V

-

E

-

G

-

H

-

M

-

G

-

Y

-

V

W

Y

D

K

A

D

G

G

N

K

P

A

D

I

E

E

T

N

Q

G

P

-

I

I

T

S

G

G

N

G

Q

E

T

A

Y

G

V

K

R

T

N

W

S

P

P

G

N

D

M

L

W

W

S

K

V

T

F

G

S

G

V

A

D

A

E

P

K
x
W

L

L

G

G

M

G

V

Y

Y

Y

L

D

P

P

-

E

-

T

-

N

-

L

-

I

-

L

L

F

G

G

N

T

Q

G

T
x
N

P

P

D

A

Q

P

W

W

G

N

G

S

M

H

R

L

T

R

P

P

A

G

S

D

E

N

K

L

V

Y

A

S

G

S

V

R

V

L

A

A

L

L

D

N

L
x
P

A
x
D

T

D

G

G

K

T

M

I

R

K

W

W

N

H

Y

F

Q

Q

F

S

T

T

H

P

H

H

D

D

L

G

W

W

D
|
D

M

F

D
|
D

V

-

G

-

G

G

Q

V

P

N

S

E

L

L

I

I

D

S

L

F

Q

N

T

Y

P

K

S

D

G

G

V

G

Q

K

P

E

A

V

L

K

I

A

A

A

S

A

T

T

-

A

-

D

K
x
R

Q

N

G

G

S

F

L

F

Y

Y

V

V

L

L

N

D

R

R

E

T

T

N

G

G

K

K

P

F

I

I

active site: E172 (= E454), N254 (≠ T518), D296 (= D560)
binding calcium ion: N161 (≠ S443), K163 (≠ H445), P278 (≠ L542), D279 (≠ A543)
binding pyrroloquinoline quinone: S154 (= S429), G170 (≠ T452), G171 (≠ N453), E172 (= E454), W236 (≠ K506), D298 (= D562), R323 (≠ K587)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 60, 104, 105, 108, 110, 390, 466, 529, 530

Query Sequence

>H281DRAFT_03298 FitnessBrowser__Burk376:H281DRAFT_03298
MSKPHRFPPALAVPAVVFIIIGLALAGGGVPLVALGGSWYYVVTGIAIALTGVLLSIRRR
SALWLFALILFGSTIWAVVEARFDFWQLLPRLWVWLVLALWLLLPPVTRKLVFGPPAAHR
EGVVPLTAAVILTVLLGLVTAFNHPYDRAGTLASTAAPPTTPIAGDANRQAADWTDYGGS
PLGQRYSPLTQITPENAGQLKVAWQFETGDKPGPGDPTETTDENTPIKVGNKLFLCTPHS
IVIALDPASGKELWRYDPHIQSPVGFKHWEHMTCRGVSYHDDAMYPANAAVAAADNAAAS
APTEGAAAGAPASTDASAAASNPQASASAPAADGASATTDAANGASTAAGAEQSASAATA
ASDTSAATQQTAAAAVSAECPRRIFLPTADARLIALNADTGQPCTHFGNNGQIDLRTNIG
PFTPGGYYSTSPPAVTRDLVIISGHVTDNESTNEPSGVTRAFDVHDGHLVWNWDAGNPDE
TQPITGNQTYVRNSPNMWSVFSVDEKLGMVYLPLGNQTPDQWGGMRTPASEKVAAGVVAL
DLATGKMRWNYQFTHHDLWDMDVGGQPSLIDLQTPSGVQPALIASTKQGSLYVLNRETGK
PIVPITEEPVPQGAGTGDHTSPTQPFSALNFKPPKVRERDMWGTNPFDQLWCRVKFKSLR
YDGMFTPPSEQGSLVFPGNFGVFDWGGIAVDPVRQILIANPSYMAFTSKLVPRSQIPSDN
GEKKGSETSGIKLARGTPYGFELNAFLSPLGIPCQAPPWGYVAGVDLRTNHIVWEHKNGT
IRDSAPLPIPMPLGVPSLGGMITTAGGVAFLSGTLDYYLRAYDVRTGDRLWQARLPAGGQ
ATPMTYADSNGKQYVLVTAGGHGSLGTKQGDYVIAYTLP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory