SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_03348 H281DRAFT_03348 amino acid ABC transporter ATP-binding protein, PAAT family to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3c4jA Abc protein artp in complex with atp-gamma-s
61% identity, 95% coverage: 11:255/258 of query aligns to 3:242/242 of 3c4jA

query
sites
3c4jA

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binding phosphothiophosphoric acid-adenylate ester: F13 (= F21), V18 (= V26), P37 (= P45), S38 (= S46), G39 (= G47), S40 (= S48), G41 (= G49), K42 (= K50), S43 (= S51), T44 (= T52), H133 (≠ D146), Y135 (≠ M148), E164 (= E177)

3c41J Abc protein artp in complex with amp-pnp/mg2+
61% identity, 95% coverage: 11:255/258 of query aligns to 3:242/242 of 3c41J

query
sites
3c41J

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binding phosphoaminophosphonic acid-adenylate ester: F13 (= F21), V18 (= V26), P37 (= P45), S38 (= S46), G39 (= G47), S40 (= S48), G41 (= G49), K42 (= K50), S43 (= S51), T44 (= T52), R47 (= R55), H133 (≠ D146), Y135 (≠ M148)
binding magnesium ion: S43 (= S51), D163 (= D176)

2olkA Abc protein artp in complex with adp-beta-s
61% identity, 95% coverage: 11:255/258 of query aligns to 3:242/242 of 2olkA

query
sites
2olkA

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binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F21), L16 (≠ H24), V18 (= V26), S38 (= S46), G39 (= G47), S40 (= S48), G41 (= G49), K42 (= K50), S43 (= S51), T44 (= T52)

2oljA Abc protein artp in complex with adp/mg2+
61% identity, 95% coverage: 11:255/258 of query aligns to 3:242/242 of 2oljA

query
sites
2oljA

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binding adenosine-5'-diphosphate: F13 (= F21), V18 (= V26), S38 (= S46), G39 (= G47), S40 (= S48), G41 (= G49), K42 (= K50), S43 (= S51), T44 (= T52), H133 (≠ D146), Y135 (≠ M148)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
59% identity, 95% coverage: 10:255/258 of query aligns to 1:240/241 of 4u00A

query
sites
4u00A

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L

binding adenosine-5'-diphosphate: F12 (= F21), L15 (≠ H24), V17 (= V26), S37 (= S46), G38 (= G47), S39 (= S48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
57% identity, 95% coverage: 11:255/258 of query aligns to 1:240/240 of 4ymuJ

query
sites
4ymuJ

I

M

V

I

S

F

I

V

R

N

G

D

L

V

T

Y

K

K

S

N

F
|
F

G

G

S

S

H
x
L

T

E

V
|
V

L

L

N

K

G

G

I

V

D

T

F

L

D

K

I

V

H

N

P

K

Q

G

Q

E

V

V

V

I

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

C

C

I

N

N

G

L

L

L

E
|
E

Q

E

A

P

E

T

R

K

G

G

T

E

I

V

D

F

I

I

C

D

G

G

H

V

R

K

L

-

V

I

D

N

Q

N

G

G

A

K

M

V

L

-

K

-

E

-

R

-

S

N

L

I

N

N

A

K

L

V

R

R

T

Q

E

K

V

V

G

G

M

M

V

V

F

F

Q

Q

S

H

F

F

N

N

L

L

F

F

P

P

H

H

L

L

S

T

V

A

L

I

H

E

N

N

I

I

T

T

V

L

G

A

P

P

R

V

M

K

L

V

R

K

G

K

A

M

S

N

K

K

A

K

Q

E

A

A

E

E

A

E

A

L

A

A

I

V

A

D

L

L

E

A

K

K

V

V

G

G

L

L

A

L

H

D

K

K

A

K

D

D

V

Q

M

Y

P

P

A

I

S

K

L

L

S

S

G

G

Q

Q

K

K

Q

Q

R

R

V

L

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

Q

Q

P

P

R

E

V

V

M

M

L

L

F

F

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

M

V

V

G

K

E

E

V

V

L

L

Q

N

V

V

M

M

K

K

L

Q

L

L

A

A

S

N

E

E

G

G

M

M

T

T

M

M

V

V

T

T

H
|
H

E

E

M

M

G

G

F

F

A

A

K

R

E

E

V

V

A

G

D

D

V

R

V

V

V

I

V

F

M

M

D

D

G

D

G

G

V

V

I

I

V

V

E

E

A

E

G

G

P

T

P

P

A

E

E

E

I

I

F

F

S

Y

A

R

P

A

S

K

Q

N

P

E

R

R

T

T

R

R

A

E

F

F

L

L

Q

S

A

K

V

I

L

L

binding adenosine-5'-triphosphate: F11 (= F21), L14 (≠ H24), V16 (= V26), S36 (= S46), G37 (= G47), S38 (= S48), G39 (= G49), K40 (= K50), S41 (= S51), T42 (= T52), E51 (= E61), E162 (= E177), H194 (= H209)
binding magnesium ion: S41 (= S51), E162 (= E177)

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
50% identity, 93% coverage: 17:257/258 of query aligns to 8:255/258 of 1b0uA

query
sites
1b0uA

L

L

T

H

K

K

S

R

F
x
Y

G

G

S

G

H
|
H

T

E

V
|
V

L

L

N

K

G

G

I

V

D

S

F

L

D

Q

I

A

H

R

P

A

Q

G

Q

D

V

V

V

I

V

S

V

I

I

I

G

G

P

S

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

C

I

N

N

G

F

L

L

E

E

Q

K

A

P

E

S

R

E

G

G

T

A

I

I

D

I

I

V

C

N

G

G

H

Q

R

N

L

I

-

N

-

L

-

V

V

R

D

D

Q

K

G

D

A

G

M

Q

L

L

K

K

-

V

-

A

-

D

E

K

R

N

S

Q

L

L

N

R

A

L

L

L

R

R

T

T

E

R

V

L

G

T

M

M

V

V

F

F

Q

Q

S

H

F

F

N

N

L

L

F

W

P

S

H

H

L

M

S

T

V

V

L

L

H

E

N

N

I

V

T

M

V

E

G

A

P

P

R

I

M

Q

L

V

R

L

G

G

A

L

S

S

K

K

A

H

Q

D

A

A

E

R

A

E

A

R

A

A

I

L

A

K

L

Y

L

L

E

A

K

K

V

V

G

G

L

I

A

D

H

E

K

R

A

A

D

Q

-

G

V

K

M

Y

P

P

A

V

S

H

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

A

M

M

Q

E

P

P

R

D

V

V

M

L

L

L

F

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

L

V

V

G

G

E

E

V

V

L

L

Q

R

V

I

M

M

K

Q

L

Q

L

L

A

A

S

E

E

E

G

G

M

K

T

T

M

M

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

K

R

E

H

V

V

A

S

D

S

V

H

V

V

V

I

V

F

M

L

D

H

G

Q

G

G

V

K

I

I

V

E

E

E

A

E

G

G

P

D

P

P

A

E

E

Q

I

V

F

F

S

G

A

N

P

P

S

Q

Q

S

P

P

R

R

T

L

R

Q

A

Q

F

F

L

L

Q

K

A

G

V

S

L

L

S

K

R

K

binding adenosine-5'-triphosphate: Y12 (≠ F21), H15 (= H24), V17 (= V26), S37 (= S46), G38 (= G47), S39 (= S48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 95% coverage: 11:255/258 of query aligns to 1:245/343 of P30750

query
sites
P30750

I

M

V

I

S

K

I

L

R

S

G

N

L

I

T

T

K

K

S

V

F

F

-

H

-

Q

G

G

S

T

H

R

T

T

V

I

-

Q

-

A

L

L

N

N

G

N

I

V

D

S

F

L

D

H

I

V

H

P

P

A

Q

G

Q

Q

V

I

V

Y

V

G

V

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

C

V

N

N

G

L

L

L

E

E

Q

R

A

P

E

T

R

E

G

G

T

S

I

V

D

L

I

V

C

D

G

G

H

Q

R

E

L

L

V

-

D

-

Q

-

G

-

A

T

M

T

L

L

K

S

E

E

R

S

S

E

L

L

N

T

A

K

L

A

R

R

T

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

S

H

F

F

N

N

L

L

F

L

P

S

H

S

L

R

S

T

V

V

L

F

H

G

N

N

I

V

T

A

V

L

G

-

P

P

R

L

M

E

L

L

R

D

G

N

A

T

S

P

K

K

A

D

Q

E

A

V

E

K

A

R

A

V

I

T

A

E

L

L

E

S

K

L

V

V

G

G

L

L

A

G

H

D

K

K

A

H

D

D

V

S

M

Y

P

P

A

S

S

N

L

L

S

S

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

Q

N

P

P

R

K

V

V

M

L

L

L

F

C

D

D

E
|
E

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

Q

E

V

L

M

L

K

K

L

D

L

I

A

N

S

R

E

R

-

L

G

G

M

L

T

T

M

I

V

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

K

K

E

R

V

I

A

C

D

D

V

C

V

V

V

A

V

V

M

I

D

S

G

N

G

G

V

E

I

L

V

I

E

E

A

Q

G

D

P

T

P

V

A

S

E

E

I

V

F

F

S

S

A

H

P

P

S

K

Q

T

P

P

R

L

T

A

R

Q

A

K

F

F

L

I

Q

Q

A

S

V

T

L

L

40:46 (vs. 46:52, 86% identical) binding
E166 (= E177) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
39% identity, 95% coverage: 11:255/258 of query aligns to 2:246/344 of 3tuzC

query
sites
3tuzC

I

M

V

I

S

K

I

L

R

S

G

N

L

I

T

T

K

K

S

V

F
|
F

-

H

-
x
Q

G

G

S

T

H

R

T

T

V

I

-

Q

-
x
A

L

L

N

N

G

N

I

V

D

S

F

L

D

H

I

V

H

P

P

A

Q

G

Q

Q

V

I

V

Y

V

G

V

V

I

I

G

G

P

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

C

V

N

N

G

L

L

L

E

E

Q

R

A

P

E

T

R

E

G

G

T

S

I

V

D

L

I

V

C

D

G

G

H

Q

R

E

L

L

V

-

D

-

Q

-

G

-

A

T

M

T

L

L

K

S

E

E

R

S

S

E

L

L

N

T

A

K

L

A

R

R

T

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

S

H

F

F

N

N

L

L

F

L

P

S

H

S

L

R

S

T

V

V

L

F

H

G

N

N

I

V

T

A

V

L

G

-

P

P

R

L

M

E

L

L

R

D

G

N

A

T

S

P

K

K

A

D

Q

E

A

V

E

K

A

R

A

V

I

T

A

E

L

L

E

S

K

L

V

V

G

G

L

L

A

G

H

D

K

K

A

H

D

D

V

S

M

Y

P

P

A

S

S

N

L

L

S

S

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

Q

N

P

P

R

K

V

V

M

L

L

L

F

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

Q

E

V

L

M

L

K

K

L

D

L

I

A

N

S

R

E

R

-

L

G

G

M

L

T

T

M

I

V

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

K

K

E

R

V

I

A

C

D

D

V

C

V

V

V

A

V

V

M

I

D

S

G

N

G

G

V

E

I

L

V

I

E

E

A

Q

G

D

P

T

P

V

A

S

E

E

I

V

F

F

S

S

A

H

P

P

S

K

Q

T

P

P

R

L

T

A

R

Q

A

K

F

F

L

I

Q

Q

A

S

V

T

L

L

binding adenosine-5'-diphosphate: F12 (= F21), Q14 (vs. gap), A21 (vs. gap), G42 (= G47), A43 (≠ S48), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 312, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
39% identity, 95% coverage: 11:255/258 of query aligns to 2:246/344 of 3tuiC

query
sites
3tuiC

I

M

V

I

S

K

I

L

R

S

G

N

L

I

T

T

K

K

S

V

F
|
F

-

H

-
x
Q

G

G

S

T

H

R

T

T

V

I

-

Q

-

A

L

L

N

N

G

N

I

V

D

S

F

L

D

H

I

V

H

P

P

A

Q

G

Q

Q

V

I

V

Y

V

G

V

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

C

V

N

N

G

L

L

L

E

E

Q

R

A

P

E

T

R

E

G

G

T

S

I

V

D

L

I

V

C

D

G

G

H

Q

R

E

L

L

V

-

D

-

Q

-

G

-

A

T

M

T

L

L

K

S

E

E

R

S

S

E

L

L

N

T

A

K

L

A

R

R

T

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

S

H

F

F

N

N

L

L

F

L

P

S

H

S

L

R

S

T

V

V

L

F

H

G

N

N

I

V

T

A

V

L

G

-

P

P

R

L

M

E

L

L

R

D

G

N

A

T

S

P

K

K

A

D

Q

E

A

V

E

K

A

R

A

V

I

T

A

E

L

L

E

S

K

L

V

V

G

G

L

L

A

G

H

D

K

K

A

H

D

D

V

S

M

Y

P

P

A

S

S

N

L

L

S

S

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

Q

N

P

P

R

K

V

V

M

L

L

L

F

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

Q

E

V

L

M

L

K

K

L

D

L

I

A

N

S

R

E

R

-

L

G

G

M

L

T

T

M

I

V

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

K

K

E

R

V

I

A

C

D

D

V

C

V

V

V

A

V

V

M

I

D

S

G

N

G

G

V

E

I

L

V

I

E

E

A

Q

G

D

P

T

P

V

A

S

E

E

I

V

F

F

S

S

A

H

P

P

S

K

Q

T

P

P

R

L

T

A

R

Q

A

K

F

F

L

I

Q

Q

A

S

V

T

L

L

binding adenosine-5'-diphosphate: F12 (= F21), Q14 (vs. gap), S41 (= S46), G42 (= G47), A43 (≠ S48), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
39% identity, 95% coverage: 11:255/258 of query aligns to 2:246/344 of 6cvlD

query
sites
6cvlD

I

M

V

I

S

K

I

L

R

S

G

N

L

I

T

T

K

K

S

V

F
|
F

-

H

-
x
Q

G

G

S

T

H

R

T

T

V
x
I

-

Q

-

A

L

L

N

N

G

N

I

V

D

S

F

L

D

H

I

V

H

P

P

A

Q

G

Q

Q

V

I

V

Y

V

G

V

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

C

V

N

N

G

L

L

L

E

E

Q

R

A

P

E

T

R

E

G

G

T

S

I

V

D

L

I

V

C

D

G

G

H

Q

R

E

L

L

V

-

D

-

Q

-

G

-

A

T

M

T

L

L

K

S

E

E

R

S

S

E

L

L

N

T

A

K

L

A

R

R

T

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

S

H

F

F

N

N

L

L

F

L

P

S

H

S

L

R

S

T

V

V

L

F

H

G

N

N

I

V

T

A

V

L

G

-

P

P

R

L

M

E

L

L

R

D

G

N

A

T

S

P

K

K

A

D

Q

E

A

V

E

K

A

R

A

V

I

T

A

E

L

L

E

S

K

L

V

V

G

G

L

L

A

G

H

D

K

K

A

H

D

D

V

S

M

Y

P

P

A

S

S
x
N

L
|
L

S
|
S

G
|
G

Q
|
Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

Q

N

P

P

R

K

V

V

M

L

L

L

F

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

Q

E

V

L

M

L

K

K

L

D

L

I

A

N

S

R

E

R

-

L

G

G

M

L

T

T

M

I

V

L

V

I

T

T

H
|
H

E

E

M

M

G

D

F

V

A

V

K

K

E

R

V

I

A

C

D

D

V

C

V

V

V

A

V

V

M

I

D

S

G

N

G

G

V

E

I

L

V

I

E

E

A

Q

G

D

P

T

P

V

A

S

E

E

I

V

F

F

S

S

A

H

P

P

S

K

Q

T

P

P

R

L

T

A

R

Q

A

K

F

F

L

I

Q

Q

A

S

V

T

L

L

binding phosphothiophosphoric acid-adenylate ester: F12 (= F21), Q14 (vs. gap), I19 (≠ V26), S41 (= S46), G42 (= G47), A43 (≠ S48), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52), N141 (≠ S151), L142 (= L152), S143 (= S153), G144 (= G154), G145 (= G155), Q146 (= Q156), H200 (= H209)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 93% coverage: 12:251/258 of query aligns to 4:234/369 of P19566

query
sites
P19566

V

V

S

Q

I

L

R

R

G

N

L

V

T

T

K

K

S

A

F

W

G

G

S

D

H

V

T

V

V

V

L

S

N

K

G

D

I

I

D

N

F

L

D

D

I

I

H

H

P

D

Q

G

Q

E

V

F

V

V

V

F

I

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

S

S

T

T

F

L

L

L

R

R

C

M

C

I

N

A

G

G

L

L

E

E

Q

T

A

I

E

T

R

S

G

G

T

D

I

L

D

F

I

I

C

G

G

E

H

T

R

R

L

M

V

N

D

D

Q

-

G

-

A

-

M

-

L

-

K

-

E

-

R

-

S

-

L

I

N

P

A

P

L

A

R

E

T

R

E

G

V

V

G

G

M

M

V

V

F

F

Q

Q

S

S

F

Y

N

A

L
|
L

F

Y

P

P

H

H

L

L

S

S

V

V

L

A

H

E

N

N

I

M

T

S

V

F

G

G

P

L

R

K

M

-

L

L

R

A

G

G

A

A

S

K

K

K

A

E

Q

V

A

M

E

N

A

Q

A

R

A

V

I

N

A

Q

L

V

L

A

E

E

K

V

V

L

G

Q

L

L

A

A

H

H

K

L

A

L

D

E

V

R

M

K

P

P

A

K

S

A

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

A

V

M

A

Q

E

P

P

R

R

V

V

M

F

L

L

F

L

D

D

E

E

P
|
P

T

L

S

S

A

N

L

L

D
|
D

P

A

E

A

L

L

-

R

V

V

G

Q

E

M

V

R

L

I

Q

E

V

I

M

S

K

R

L

L

L

H

A

K

S

R

E

L

G

G

M

R

T

T

M

M

V

I

V

Y

V

V

T

T

H

H

E

D

M

Q

G

V

F

E

A

A

K

M

E

T

V

L

A

A

D

D

V

K

V

I

V

V

M

L

D

D

G

A

G

G

V

R

I

V

V

A

E

Q

A

V

G

G

P

K

P

P

A

L

E

E

I

L

F

Y

S

H

A

Y

P

P

S

A

Q

D

P

R

R

F

T

V

R

A

A

G

F

F

L

I

L86 (= L103) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P178) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D183) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
40% identity, 93% coverage: 12:251/258 of query aligns to 4:234/371 of P68187

query
sites
P68187

V

V

S

Q

I

L

R

Q

G

N

L

V

T

T

K

K

S

A

F

W

G

G

S

E

H

V

T

V

V

V

L

S

N

K

G

D

I

I

D

N

F

L

D

D

I

I

H

H

P

E

Q

G

Q

E

V

F

V

V

V

F

I

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

S

S

T

T

F

L

L

L

R

R

C

M

C

I

N

A

G

G

L

L

E

E

Q

T

A

I

E

T

R

S

G

G

T

D

I

L

D

F

I

I

C

G

G

E

H

K

R

R

L

M

V

N

D

D

Q

T

G

P

A

P

M

-

L

-

K

A

E

E

R

R

S

G

L

-

N

-

A

-

L

-

R

-

T

-

E

-

V

V

G

G

M

M

V

V

F

F

Q

Q

S

S

F

Y

N
x
A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

L

A

H

E

N

N

I

M

T

S

V

F

G

G

P

L

R

K

M

-

L

L

R

A

G

G

A

A

S
x
K

K

K

A

E

Q

V

A

I

E

N

A

Q

A

R

A
x
V

I

N

A

Q

L
x
V

L

A

E
|
E

K

V

V

L

G

Q

L

L

A
|
A

H

H

K

L

A

L

D

D

V

R

M

K

P

P

A

K

S

A

L

L

S

S

G

G

G
|
G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

A

V

M

A

Q

E

P

P

R

S

V

V

M

F

L

L

F

L

D
|
D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

P

A

E

A

L

L

-

R

V

V

G

Q

E

M

V

R

L

I

Q

E

V

I

M

S

K

R

L

L

L

H

A

K

S

R

E

L

G

G

M

R

T

T

M

M

V

I

V

Y

V

V

T

T

H

H

E

D

M

Q

G

V

F

E

A

A

K

M

E

T

V

L

A

A

D

D

V

K

V

I

V

V

M

L

D

D

G

A

G

G

V

R

I

V

V

A

E

Q

A

V

G

G

P

K

P

P

A

L

E

E

I

L

F

Y

S

H

A

Y

P

P

S

A

Q

D

P
x
R

R

F

T

V

R

A

A

G

F

F

L

I

A85 (≠ N102) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ S124) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ A132) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L135) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E137) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A142) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G155) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D176) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ P245) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
40% identity, 93% coverage: 12:251/258 of query aligns to 3:233/371 of 3puyA

query
sites
3puyA

V

V

S

Q

I

L

R

Q

G

N

L

V

T

T

K

K

S

A

F
x
W

G

G

S

E

H

V

T

V

V
|
V

L

S

N

K

G

D

I

I

D

N

F

L

D

D

I

I

H

H

P

E

Q

G

Q

E

V

F

V

V

V

F

I

V

G

G

P
|
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

M

C

I

N

A

G

G

L

L

E

E

Q

T

A

I

E

T

R

S

G

G

T

D

I

L

D

F

I

I

C

G

G

E

H

K

R

R

L

M

V

N

D

D

Q

T

G

P

A

P

M

-

L

-

K

A

E

E

R

R

S

G

L

-

N

-

A

-

L

-

R

-

T

-

E

-

V

V

G

G

M

M

V

V

F

F

Q
|
Q

S

S

F

Y

N

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

L

A

H

E

N

N

I

M

T

S

V

F

G

G

P

L

R

K

M

-

L

L

R

A

G

G

A

A

S

K

K

K

A

E

Q

V

A

I

E

N

A

Q

A

R

A

V

I

N

A

Q

L

V

L

A

E

E

K

V

V

L

G

Q

L

L

A

A

H

H

K

L

A

L

D

D

V
x
R

M

K

P

P

A

K

S
x
A

L

L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

A

V

M

A

Q

E

P

P

R

S

V

V

M

F

L

L

F

L

D
|
D

E
|
E

P

P

T

L

S

S

A

N

L

L

D

D

P

A

E

A

L

L

-

R

V

V

G

Q

E

M

V

R

L

I

Q

E

V

I

M

S

K

R

L

L

L

H

A

K

S

R

E

L

G

G

M

R

T

T

M

M

V

I

V

Y

V

V

T

T

H
|
H

E

D

M

Q

G

V

F

E

A

A

K

M

E

T

V

L

A

A

D

D

V

K

V

I

V

V

M

L

D

D

G

A

G

G

V

R

I

V

V

A

E

Q

A

V

G

G

P

K

P

P

A

L

E

E

I

L

F

Y

S

H

A

Y

P

P

S

A

Q

D

P

R

R

F

T

V

R

A

A

G

F

F

L

I

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F21), V17 (= V26), P36 (= P45), S37 (= S46), G38 (= G47), C39 (≠ S48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), Q81 (= Q99), R128 (≠ V147), A132 (≠ S151), S134 (= S153), G135 (= G154), G136 (= G155), Q137 (= Q156), E158 (= E177), H191 (= H209)
binding magnesium ion: S42 (= S51), Q81 (= Q99), D157 (= D176)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
40% identity, 93% coverage: 12:251/258 of query aligns to 3:233/371 of 3puxA

query
sites
3puxA

V

V

S

Q

I

L

R

Q

G

N

L

V

T

T

K

K

S

A

F
x
W

G

G

S

E

H

V

T

V

V
|
V

L

S

N

K

G

D

I

I

D

N

F

L

D

D

I

I

H

H

P

E

Q

G

Q

E

V

F

V

V

V

F

I

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

M

C

I

N

A

G

G

L

L

E

E

Q

T

A

I

E

T

R

S

G

G

T

D

I

L

D

F

I

I

C

G

G

E

H

K

R

R

L

M

V

N

D

D

Q

T

G

P

A

P

M

-

L

-

K

A

E

E

R

R

S

G

L

-

N

-

A

-

L

-

R

-

T

-

E

-

V

V

G

G

M

M

V

V

F

F

Q
|
Q

S

S

F

Y

N

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

L

A

H

E

N

N

I

M

T

S

V

F

G

G

P

L

R

K

M

-

L

L

R

A

G

G

A

A

S

K

K

K

A

E

Q

V

A

I

E

N

A

Q

A

R

A

V

I

N

A

Q

L

V

L

A

E

E

K

V

V

L

G

Q

L

L

A

A

H

H

K

L

A

L

D

D

V
x
R

M

K

P

P

A

K

S
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

A

V

M

A

Q

E

P

P

R

S

V

V

M

F

L

L

F

L

D
|
D

E
|
E

P

P

T

L

S

S

A

N

L

L

D

D

P

A

E

A

L

L

-

R

V

V

G

Q

E

M

V

R

L

I

Q

E

V

I

M

S

K

R

L

L

L

H

A

K

S

R

E

L

G

G

M

R

T

T

M

M

V

I

V

Y

V

V

T

T

H
|
H

E

D

M

Q

G

V

F

E

A

A

K

M

E

T

V

L

A

A

D

D

V

K

V

I

V

V

M

L

D

D

G

A

G

G

V

R

I

V

V

A

E

Q

A

V

G

G

P

K

P

P

A

L

E

E

I

L

F

Y

S

H

A

Y

P

P

S

A

Q

D

P

R

R

F

T

V

R

A

A

G

F

F

L

I

binding adenosine-5'-diphosphate: W12 (≠ F21), V17 (= V26), S37 (= S46), G38 (= G47), C39 (≠ S48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), R128 (≠ V147), A132 (≠ S151), L133 (= L152), S134 (= S153), Q137 (= Q156)
binding beryllium trifluoride ion: S37 (= S46), G38 (= G47), K41 (= K50), Q81 (= Q99), S134 (= S153), G135 (= G154), G136 (= G155), E158 (= E177), H191 (= H209)
binding magnesium ion: S42 (= S51), Q81 (= Q99), D157 (= D176)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
40% identity, 93% coverage: 12:251/258 of query aligns to 3:233/371 of 3puwA

query
sites
3puwA

V

V

S

Q

I

L

R

Q

G

N

L

V

T

T

K

K

S

A

F
x
W

G

G

S

E

H

V

T

V

V
|
V

L

S

N

K

G

D

I

I

D

N

F

L

D

D

I

I

H

H

P

E

Q

G

Q

E

V

F

V

V

V

F

I

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

M

C

I

N

A

G

G

L

L

E

E

Q

T

A

I

E

T

R

S

G

G

T

D

I

L

D

F

I

I

C

G

G

E

H

K

R

R

L

M

V

N

D

D

Q

T

G

P

A

P

M

-

L

-

K

A

E

E

R

R

S

G

L

-

N

-

A

-

L

-

R

-

T

-

E

-

V

V

G

G

M

M

V

V

F

F

Q
|
Q

S

S

F

Y

N

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

L

A

H

E

N

N

I

M

T

S

V

F

G

G

P

L

R

K

M

-

L

L

R

A

G

G

A

A

S

K

K

K

A

E

Q

V

A

I

E

N

A

Q

A

R

A

V

I

N

A

Q

L

V

L

A

E

E

K

V

V

L

G

Q

L

L

A

A

H

H

K

L

A

L

D

D

V
x
R

M

K

P

P

A

K

S
x
A

L

L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

A

V

M

A

Q

E

P

P

R

S

V

V

M

F

L

L

F

L

D

D

E
|
E

P

P

T

L

S

S

A
x
N

L

L

D

D

P

A

E

A

L

L

-

R

V

V

G

Q

E

M

V

R

L

I

Q

E

V

I

M

S

K

R

L

L

L

H

A

K

S

R

E

L

G

G

M

R

T

T

M

M

V

I

V

Y

V

V

T

T

H
|
H

E

D

M

Q

G

V

F

E

A

A

K

M

E

T

V

L

A

A

D

D

V

K

V

I

V

V

M

L

D

D

G

A

G

G

V

R

I

V

V

A

E

Q

A

V

G

G

P

K

P

P

A

L

E

E

I

L

F

Y

S

H

A

Y

P

P

S

A

Q

D

P

R

R

F

T

V

R

A

A

G

F

F

L

I

binding adenosine-5'-diphosphate: W12 (≠ F21), V17 (= V26), S37 (= S46), G38 (= G47), C39 (≠ S48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), R128 (≠ V147), A132 (≠ S151), S134 (= S153), Q137 (= Q156)
binding tetrafluoroaluminate ion: S37 (= S46), G38 (= G47), K41 (= K50), Q81 (= Q99), S134 (= S153), G135 (= G154), G136 (= G155), E158 (= E177), N162 (≠ A181), H191 (= H209)
binding magnesium ion: S42 (= S51), Q81 (= Q99)

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
40% identity, 93% coverage: 12:251/258 of query aligns to 3:233/372 of 2awoA

query
sites
2awoA

V

V

S

Q

I

L

R

Q

G

N

L

V

T

T

K

K

S

A

F
x
W

G

G

S

E

H

V

T

V

V
|
V

L

S

N

K

G

D

I

I

D

N

F

L

D

D

I

I

H

H

P

E

Q

G

Q

E

V

F

V

V

V

F

I

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

M

C

I

N

A

G

G

L

L

E

E

Q

T

A

I

E

T

R

S

G

G

T

D

I

L

D

F

I

I

C

G

G

E

H

K

R

R

L

M

V

N

D

D

Q

T

G

P

A

P

M

-

L

-

K

A

E

E

R

R

S

G

L

-

N

-

A

-

L

-

R

-

T

-

E

-

V

V

G

G

M

M

V

V

F

F

Q

Q

S

S

F

Y

N

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

L

A

H

E

N

N

I

M

T

S

V

F

G

G

P

L

R

K

M

-

L

L

R

A

G

G

A

A

S

K

K

K

A

E

Q

V

A

I

E

N

A

Q

A

R

A

V

I

N

A

Q

L

V

L

A

E

E

K

V

V

L

G

Q

L

L

A

A

H

H

K

L

A

L

D

D

V

R

M

K

P

P

A

K

S

A

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

A

V

M

A

Q

E

P

P

R

S

V

V

M

F

L

L

F

L

D
|
D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

P

A

E

A

L

L

-

R

V

V

G

Q

E

M

V

R

L

I

Q

E

V

I

M

S

K

R

L

L

L

H

A

K

S

R

E

L

G

G

M

R

T

T

M

M

V

I

V

Y

V

V

T

T

H

H

E

D

M

Q

G

V

F

E

A

A

K

M

E

T

V

L

A

A

D

D

V

K

V

I

V

V

M

L

D

D

G

A

G

G

V

R

I

V

V

A

E

Q

A

V

G

G

P

K

P

P

A

L

E

E

I

L

F

Y

S

H

A

Y

P

P

S

A

Q

D

P

R

R

F

T

V

R

A

A

G

F

F

L

I

binding adenosine-5'-diphosphate: W12 (≠ F21), V17 (= V26), S37 (= S46), G38 (= G47), C39 (≠ S48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52)
binding magnesium ion: S42 (= S51), D157 (= D176)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
40% identity, 93% coverage: 12:251/258 of query aligns to 1:231/367 of 1q12A

query
sites
1q12A

V

V

S

Q

I

L

R

Q

G

N

L

V

T

T

K

K

S

A

F
x
W

G

G

S

E

H

V

T

V

V
|
V

L

S

N

K

G

D

I

I

D

N

F

L

D

D

I

I

H

H

P

E

Q

G

Q

E

V

F

V

V

V

F

I

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

M

C

I

N

A

G

G

L

L

E

E

Q

T

A

I

E

T

R

S

G

G

T

D

I

L

D

F

I

I

C

G

G

E

H

K

R

R

L

M

V

N

D

D

Q

T

G

P

A

P

M

-

L

-

K

A

E

E

R

R

S

G

L

-

N

-

A

-

L

-

R

-

T

-

E

-

V

V

G

G

M

M

V

V

F

F

Q

Q

S

S

F

Y

N

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

L

A

H

E

N

N

I

M

T

S

V

F

G

G

P

L

R

K

M

-

L

L

R

A

G

G

A

A

S

K

K

K

A

E

Q

V

A

I

E

N

A

Q

A

R

A

V

I

N

A

Q

L

V

L

A

E

E

K

V

V

L

G

Q

L

L

A

A

H

H

K

L

A

L

D

D

V
x
R

M

K

P

P

A
x
K