SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_03486 FitnessBrowser__Burk376:H281DRAFT_03486 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

A0A0H2ZQB9 Ergothioneine transporter EgtUBC from Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (see paper)
42% identity, 65% coverage: 72:227/239 of query aligns to 47:202/506 of A0A0H2ZQB9

query
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A0A0H2ZQB9

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Sites not aligning to the query:

244 G→F: Disrupts ergothioneine (EGT) binding. Binds EGT 100-fold more weakly. May have a global impact on overall structure.
293 F→Y: Disrupts ergothioneine (EGT) binding.
375 E→Q: Disrupts ergothioneine (EGT) binding. Binds EGT 500-fold more weakly.
419 Y→F: Disrupts ergothioneine (EGT) binding.

B5Z7I3 Ergothioneine transport permease/ergothioneine binding protein EgtU from Helicobacter pylori (strain G27) (see paper)
34% identity, 82% coverage: 32:227/239 of query aligns to 52:240/553 of B5Z7I3

query
sites
B5Z7I3

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Sites not aligning to the query:

390 Y→A: Disrupts EGT binding.; Y→F: Retains EGT binding.
454 R→A: Retains EGT binding.; R→E: Disrupts EGT binding.

Query Sequence

>H281DRAFT_03486 FitnessBrowser__Burk376:H281DRAFT_03486
MNTLLSRCALLFACAAPLQARADNWLLSIAQYRTDIVYQSITQMTMVAIAGGLAIVVAVP
LGIYLSRESGKHARAGQALLQTLNMGQAVPKLALLALAMSVLGIGRWPSIFALWVATLLP
IVLNTLEGLRAVPRALIEAATGMGMTPRQVLFRVELPNALYVIFAGIRTALAITVGTAPL
AFLVGGGGLGELIFTGIDMNDFSMLLAGAIPTAVLAVLTDVLIGQMQVHLVSRGVTPQR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory