SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_03540 FitnessBrowser__Burk376:H281DRAFT_03540 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
62% identity, 99% coverage: 2:475/479 of query aligns to 6:481/482 of P25526

query
sites
P25526

N

S

E

N

F

L

L

F

R

R

T

Q

G

Q

H

A

Y

L

I

I

G

N

G

G

E

E

W

W

Y

L

E

D

-

A

-

N

G

N

A

G

S

E

T

A

Y

I

P

D

V

V

L

T

N

N

P

P

A

A

T

N

G

G

E

D

V

K

I

L

A

G

Q

S

V

V

A

P

K

K

G

M

G

G

A

A

V

D

E

E

A

T

T

R

Q

A

A

A

I

I

A

D

A

A

E

N

R

R

A

A

F

L

P

P

A

A

W

W

R

R

S

A

L

L

T

T

A

A

K

K

E

E

R

R

S

A

A

T

R

I

V

L

K

R

R

N

W

W

G

F

E

N

L

L

M

M

L

M

E

E

H

H

R

Q

D

D

A

D

L

L

A

A

A

R

L

L

L

M

T

T

R

L

E

E

Q

Q

G

G

K

K

P

P

L

L

A

A

E

E

A

A

R

K

G

G

E

E

V

I

G

S

Y

Y

A

A

S

S

F

F

F

I

E

E

W

W

F

F

A

A

E

E

A

G

K

K

R

R

A

I

Y

Y

G

G

D

D

V

T

I

I

P

P

S

G

P

H

N

Q

P

A

N

D

A

K

K

R

I

L

I

I

V

V

T

I

R

K

E

Q

P

P

V

I

G

G

V

V

V

T

A

A

I

I

T

T

P

P

W
|
W

N
|
N

F

F

P

P

L

A

A

A

M

M

I

I

T

T

R

R

K

K

A

A

G

G

P

P

A

A

L

L

A

A

G

G

C

C

T

T

M

M

V

V

L

L

K
|
K

P
|
P

S
x
A

E
x
S

E

Q

T

T

P

P

L

F

S

S

A

A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

I

K

R

A

A

G

G

I

V

P

P

P

A

G

G

V

V

F

F

N

N

V

V

S

T

G

G

D

S

A

A

V

G

A

A

I

V

G

G

G

N

A

E

L

L

T

T

E

S

S

N

D

P

V

L

V

V

R

R

K

K

L

L

S

S

F

F

T

T

G
|
G

S
|
S

T

T

R

E

V

I

G

G

K

R

L

Q

L

L

A

M

K

E

Q

Q

S

C

A

A

D

K

T

D

L

I

K

K

L

V

S

S

L

L

E

E

L
|
L

G

G

N

N

A

A

P

P

F

F

I

I

V

V

F

F

D

D

A

A

D

D

L

L

D

D

A

K

A

A

V

V

Q

E

G

G

A

A

M

L

A

A

S

S

K

K

F

F

R

R

N

N

T

A

G

G

Q

Q

T

T

C

C

V

V

C

C

V

A

N

N

R

R

F

L

Y

Y

V

V

Q

Q

D

D

G

G

I

V

Y

Y

D

D

A

R

F

F

T

A

L

E

A

K

L

L

A

Q

Q

Q

A

A

A

V

R

S

K

K

M

L

R

H

V

I

G

G

N

D

A

G

L

L

Q

D

G

N

D

G

V

V

E

T

Q

I

G

G

P

P

L

L

I

I

N

D

Q

E

A

K

A

A

L

V

T

A

K

K

V

V

E

E

A

E

H

H

V

I

A

A

D

D

A

A

L

L

Q

E

K

K

G

G

A

A

K

R

V

V

L

V

T

C

G

G

A

G

K

K

P

A

H

H

A

E

L

R

G

G

T

N

F

F

Y

F

E

Q

P

P

T

T

V

I

L

L

V

V

D

D

A

V

S

P

S

A

S

N

M

A

L

K

I

V

A

S

Q

K

E

E

E
|
E

T

T

F

F

G

G

P

P

V

L

A

A

A

P

C

L

F

F

R

R

F

F

K

K

T

D

E

E

D

A

E

D

A

V

V

I

A

A

A

Q

A

A

N

N

A

D

T

T

P

E

F

F

G

G

L

L

S

A

A

A

Y

Y

F

F

Y

Y

T

A

R

R

D

D

L

L

A

S

R

R

A

V

W

F

R

R

V

V

G

G

E

E

A

A

L

L

E

E

S

Y

G

G

M

I

V

V

G

G

I

I

N

N

E

T

G

G

I

I

L

I

S

S

T

N

E

E

V

V

A

A

P

P

F

F

G

G

V

I

K

K

Q

A

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

L

I

D

E

E

D

Y

Y

T

L

E

E

L

I

K

K

Y

Y

M

M

M

C

M

I

G

G

WN 156:157 (= WN 150:151) binding
KPAS 180:183 (≠ KPSE 174:177) binding
GS 233:234 (= GS 227:228) binding
L256 (= L250) binding
E386 (= E380) binding

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
62% identity, 99% coverage: 2:475/479 of query aligns to 5:480/481 of 3jz4A

query
sites
3jz4A

N

S

E

N

F

L

L

F

R

R

T

Q

G

Q

H

A

Y

L

I

I

G

N

G

G

E

E

W

W

Y

L

E

D

-

A

-

N

G

N

A

G

S

E

T

A

Y

I

P

D

V

V

L

T

N

N

P

P

A

A

T

N

G

G

E

D

V

K

I

L

A

G

Q

S

V

V

A

P

K

K

G

M

G

G

A

A

V

D

E

E

A

T

T

R

Q

A

A

A

I

I

A

D

A

A

E

N

R

R

A

A

F

L

P

P

A

A

W

W

R

R

S

A

L

L

T

T

A

A

K

K

E

E

R

R

S

A

A

T

R

I

V

L

K

R

R

N

W

W

G

F

E

N

L

L

M

M

L

M

E

E

H

H

R

Q

D

D

A

D

L

L

A

A

A

R

L

L

L

M

T

T

R

L

E

E

Q

Q

G

G

K

K

P

P

L

L

A

A

E

E

A

A

R

K

G

G

E

E

V

I

G

S

Y

Y

A

A

S

S

F

F

F

I

E

E

W

W

F

F

A

A

E

E

A

G

K

K

R

R

A

I

Y

Y

G

G

D

D

V

T

I

I

P

P

S

G

P

H

N

Q

P

A

N

D

A

K

K

R

I

L

I

I

V

V

T

I

R

K

E

Q

P

P

V

I

G

G

V

V

V

T

A

A

I
|
I

T

T

P
|
P

W
|
W

N
|
N

F

F

P

P

L

A

A

A

M

M

I

I

T

T

R

R

K

K

A

A

G

G

P

P

A

A

L

L

A

A

G

G

C

C

T

T

M

M

V

V

L

L

K
|
K

P

P

S
x
A

E
x
S

E

Q

T

T

P

P

L

F

S

S

A

A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

I

K

R

A

A

G

G

I

V

P

P

P

A

G

G

V

V

F

F

N

N

V

V

S

T

G

G

D
x
S

A
|
A

V
x
G

A

A

I

V

G
|
G

G

N

A

E

L

L

T

T

E

S

S

N

D

P

V

L

V

V

R

R

K

K

L

L

S

S

F
|
F

T
|
T

G
|
G

S
|
S

T

T

R

E

V
x
I

G

G

K

R

L

Q

L

L

A

M

K

E

Q

Q

S

C

A

A

D

K

T

D

L

I

K

K

L

V

S

S

L

L

E
|
E

L
|
L

G

G

N

N

A

A

P

P

F

F

I

I

V

V

F

F

D

D

A

A

D

D

L

L

D

D

A

K

A

A

V

V

Q

E

G

G

A

A

M

L

A

A

S

S

K

K

F

F

R

R

N

N

T

A

G

G

Q

Q

T

T

C
|
C

V

V

C

C

V

A

N

N

R

R

F

L

Y

Y

V

V

Q

Q

D

D

G

G

I

V

Y

Y

D

D

A

R

F

F

T

A

L

E

A

K

L

L

A

Q

Q

Q

A

A

A

M

R

S

K

K

M

L

R

H

V

I

G

G

N

D

A

G

L

L

Q

D

G

N

D

G

V

V

E

T

Q

I

G

G

P

P

L

L

I

I

N

D

Q

E

A

K

A

A

L

V

T

A

K
|
K

V

V

E

E

A

E

H

H

V

I

A

A

D

D

A

A

L

L

Q

E

K

K

G

G

A

A

K

R

V

V

L

V

T

C

G

G

A

G

K

K

P

A

H

H

A

E

L

R

G

G

T

N

F

F

Y

F

E

Q

P

P

T

T

V

I

L

L

V

V

D

D

A

V

S

P

S

A

S

N

M

A

L

K

I

V

A

S

Q

K

E

E

E
|
E

T

T

F
|
F

G

G

P

P

V

L

A

A

A

P

C

L

F

F

R

R

F

F

K

K

T

D

E

E

D

A

E

D

A

V

V

I

A

A

A

Q

A

A

N

N

A

D

T

T

P

E

F

F

G

G

L

L

S

A

A

A

Y

Y

F

F

Y

Y

T

A

R

R

D

D

L

L

A

S

R

R

A

V

W

F

R

R

V

V

G

G

E

E

A

A

L

L

E

E

S

Y

G

G

M

I

V

V

G

G

I

I

N

N

E

T

G

G

I

I

L

I

S

S

T

N

E

E

V

V

A

A

P

P

F

F

G

G

V

I

K

K

Q

A

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

L

I

D

E

E

D

Y

Y

T

L

E

E

L

I

K

K

Y

Y

M

M

M

C

M

I

G

G

active site: N156 (= N151), K179 (= K174), E254 (= E249), C288 (= C283), E385 (= E380), E462 (= E457)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I152 (= I147), P154 (= P149), W155 (= W150), N156 (= N151), K179 (= K174), A181 (≠ S176), S182 (≠ E177), S211 (≠ D206), A212 (= A207), G213 (≠ V208), G216 (= G211), F230 (= F225), T231 (= T226), G232 (= G227), S233 (= S228), I236 (≠ V231), E254 (= E249), L255 (= L250), C288 (= C283), K338 (= K333), E385 (= E380), F387 (= F382)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
56% identity, 99% coverage: 4:477/479 of query aligns to 58:535/535 of P51649

query
sites
P51649

F

L

L

L

R

R

T

T

G

D

H

S

Y

F

I

V

G

G

E

R

W

W

Y

L

E

P

G

A

A

A

S

A

T

T

Y

F

P

P

V

V

L

Q

N

D

P

P

A

A

T

S

G

G

E

A

V

A

I

L

A

G

Q

M

V

V

A

A

K

D

G
x
C

G

G

A

V

V

R

E

E

A

A

T

R

Q

A

A

A

I

V

A

R

A

A

E

Y

R

E

A

A

F

F

P

C

A

R

W

W

R

R

S

E

L

V

T

S

A

A

K

K

E

E

R

R

S

S

A

S

R

L

V

L

K

R

R

K

W

W

G

Y

E

N

L

L

M

M

L

I

E

Q

H

N

R

K

D

D

A

D

L

L

A

A

A

R

L

I

T

T

R

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

R

H

G

G

E

E

V

I

G

L

Y

Y

A

S

A

A

S

F

F

F

F

L

E

E

W

W

F

F

A

S

E

E

A

A

K

R

R

R

A

V

Y

Y

G
|
G

D

D

V

I

I

I

P
x
H

S

T

P
|
P

N

A

P

K

N

D

A

R

K

R

I

A

I

L

V

V

T

L

R

K

E

Q

P

P

V

I

G

G

V

V

V

A

A

A

A

V

I

I

T

T

P

P

W

W

N

N

F

F

P

P

L

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C
|
C

T

T

M

V

V

V

L

V

K
|
K

P
|
P

S
x
A

E
|
E

E

D

T
|
T

P

P

L

F

S

S

A
|
A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

S

K

Q

A

A

G

G

I

I

P

P

P

S

G

G

V

V

F

Y

N
|
N

V

V

V

I

-

P

-

C

-

S

S

R

G

K

D

N

A

A

V

K

A

E

I

V

G
|
G

G

E

A

A

L

I

T

C

E

T

S

D

D

P

V

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G
|
G

S
|
S

T
|
T

R
x
T

V
x
T

G
|
G

K

K

L

I

L

L

A

L

K

H

Q

H

S

A

A

A

D

N

T

S

L

V

K

K

K

R

L

V

S

S

L

M

E

E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

A

Q

A

A

V

V

Q

A

G

G

A

A

M

M

A

A

S

S

K

K

F

F

R
|
R

N
|
N

T

T

G

G

Q

Q

T

T

C
|
C

V

V

C
|
C

V

S

N

N

R

Q

F

F

Y

L

V

V

Q

Q

D

R

G

G

I

I

Y

H

D

D

A

A

F

F

T

V

L

K

A

A

L

F

A

A

Q

E

A

A

A

M

R

K

K

K

-

N

M

L

R

R

V

V

G

G

N
|
N

A

G

L

F

Q

E

G

E

D

G

V

T

E

T

Q

Q

G

G

P
|
P

L

L

I

I

N

N

Q

E

A

K

A

A

L

V

T

E

K

K

V

V

E

E

A

K

H

Q

V

V

A

N

D

D

A

A

L

V

Q

S

K

K

G

G

A

A

K

T

V
|
V

L

V

T

T

G
|
G

A

G

K

K

P

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

Y

F

E

E

P

P

T

T

V

L

L

L

V

C

D

N

A

V

S

T

S

Q

S

D

M

M

L

L

I

C

A

T

Q

H

E

E

T

T

F

F

G

G

P

P

V

L

A

A

A

P

C

V

F

I

R

K

F

F

K

D

T

T

E

E

D

E

E

E

A

A

V

I

A

A

A

I

A

A

N

N

A

A

T

A

P

D

F

V

G

G

L

L

S

A

A

G

Y

Y

F

F

Y

Y

T

S

R

Q

D

D

L

P

A

A

R

Q

A

I

W

W

R

R

V

V

G

A

E

E

A

Q

L

L

E

E

S

V

G

G

M

M

V

V

G

G

I

V

N

N

E

E

G

G

I

L

L

I

S
|
S

T

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

L

I

D

D

E

E

Y

Y

T

L

E

E

L

L

K

K

Y

Y

M

V

M

C

M

Y

G
|
G

G

G

L

L

C93 (≠ G39) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G122) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ P126) to Y: 83% of activity; dbSNP:rs2760118
P182 (= P128) to L: 48% of activity; dbSNP:rs3765310
R213 (= R159) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C169) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (≠ KPSE 174:177) binding
T233 (= T179) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A183) to S: 65% of activity; dbSNP:rs62621664
N255 (= N201) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G211) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTRVG 227:232) binding
R334 (= R277) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N278) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C283) modified: Disulfide link with 342, In inhibited form
C342 (= C285) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (= N314) natural variant: N -> S
P382 (= P324) to L: in SSADHD; 2% of activity
V406 (= V348) to I: in dbSNP:rs143741652
G409 (= G351) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (= S440) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
G533 (= G475) to R: in SSADHD; <1% of activity; dbSNP:rs72552284

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
56% identity, 99% coverage: 4:477/479 of query aligns to 8:485/485 of 2w8rA

query
sites
2w8rA

F

L

L

L

R

R

T

T

G

D

H

S

Y

F

I

V

G

G

E

R

W

W

Y

L

E

P

G

A

A

A

S

A

T

T

Y

F

P

P

V

V

L

Q

N

D

P

P

A

A

T

S

G

G

E

A

V

A

I

L

A

G

Q

M

V

V

A

A

K

D

G

C

G

G

A

V

V

R

E

E

A

A

T

R

Q

A

A

A

I

V

A

R

A

A

E

Y

R

E

A

A

F

F

P

C

A

R

W

W

R

R

S

E

L

V

T

S

A

A

K

K

E

E

R

R

S

S

A

S

R

L

V

L

K

R

R

K

W

W

G

Y

E

N

L

L

M

M

L

I

E

Q

H

N

R

K

D

D

A

D

L

L

A

A

A

R

L

I

T

T

R

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

R

H

G

G

E

E

V

I

G

L

Y

Y

A

S

A

A

S

F

F

F

F

L

E

E

W

W

F

F

A

S

E

E

A

A

K

R

R

R

A

V

Y

Y

G

G

D

D

V

I

I

I

P

H

S

T

P

P

N

A

P

K

N

D

A

R

K

R

I

A

I

L

V

V

T

L

R

K

E

Q

P

P

V

I

G

G

V

V

V

A

A

A

A

V

I
|
I

T
|
T

P
|
P

W
|
W

N
|
N

F

F

P

P

L

S

A

A

M

M

I

I

T

T

R

R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

V

V

L

V

K
|
K

P
|
P

S
x
A

E
|
E

E

D

T

T

P

P

L

F

S

S

A

A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

S

K

Q

A

A

G

G

I

I

P

P

P

S

G

G

V

V

F

Y

N

N

V

V

V

I

-

P

-

C

-

S

S

R

G

K

D

N

A
|
A

V
x
K

A

E

I

V

G

G

G

E

A

A

L

I

T

C

E

T

S

D

D

P

V

L

V

V

R

S

K

K

L

I

S

S

F
|
F

T

T

G

G

S
|
S

T

T

R

T

V
x
T

G

G

K

K

L

I

L

L

A

L

K

H

Q

H

S

A

A

A

D

N

T

S

L

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

A

Q

A

A

V

V

Q

A

G

G

A

A

M

M

A

A

S

S

K

K

F

F

R

R

N

N

T

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

V

S

N

N

R

Q

F

F

Y

L

V

V

Q

Q

D

R

G

G

I

I

Y

H

D

D

A

A

F

F

T

V

L

K

A

A

L

F

A

A

Q

E

A

A

A

M

R

K

K

K

-

N

M

L

R

R

V

V

G

G

N

N

A

G

L

F

Q

E

G

E

D

G

V

T

E

T

Q

Q

G

G

P

P

L

L

I

I

N

N

Q

E

A

K

A

A

L

V

T

E

K

K

V

V

E

E

A

K

H

Q

V

V

A

N

D

D

A

A

L

V

Q

S

K

K

G

G

A

A

K

T

V

V

L

V

T

T

G

G

A

G

K

K

P

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

Y

F

E

E

P

P

T

T

V

L

L

L

V

C

D

N

A

V

S

T

S

Q

S

D

M

M

L

L

I

C

A

T

Q

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

L

A

A

A

P

C

V

F

I

R

K

F

F

K

D

T

T

E

E

D

E

E

E

A

A

V

I

A

A

A

I

A

A

N

N

A

A

T

A

P

D

F

V

G

G

L

L

S

A

A

G

Y

Y

F

F

Y

Y

T

S

R

Q

D

D

L

P

A

A

R

Q

A

I

W

W

R

R

V

V

G

A

E

E

A

Q

L

L

E

E

S

V

G

G

M

M

V

V

G

G

I

V

N

N

E

E

G

G

I

L

L

I

S

S

T

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

L

I

D

D

E

E

Y

Y

T

L

E

E

L

L

K

K

Y

Y

M

V

M

C

M

Y

G

G

L

L

active site: N155 (= N151), K178 (= K174), E256 (= E249), A290 (≠ C283), E388 (= E380), E465 (= E457)
binding adenosine-5'-diphosphate: I151 (= I147), T152 (= T148), P153 (= P149), W154 (= W150), K178 (= K174), P179 (= P175), A180 (≠ S176), E181 (= E177), A214 (= A207), K215 (≠ V208), F232 (= F225), S235 (= S228), T238 (≠ V231)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
56% identity, 99% coverage: 4:477/479 of query aligns to 8:485/485 of 2w8qA

query
sites
2w8qA

F

L

L

L

R

R

T

T

G

D

H

S

Y

F

I

V

G

G

E

R

W

W

Y

L

E

P

G

A

A

A

S

A

T

T

Y

F

P

P

V

V

L

Q

N

D

P

P

A

A

T

S

G

G

E

A

V

A

I

L

A

G

Q

M

V

V

A

A

K

D

G

C

G

G

A

V

V

R

E

E

A

A

T

R

Q

A

A

A

I

V

A

R

A

A

E

Y

R

E

A

A

F

F

P

C

A

R

W

W

R

R

S

E

L

V

T

S

A

A

K

K

E

E

R

R

S

S

A

S

R

L

V

L

K

R

R

K

W

W

G

Y

E

N

L

L

M

M

L

I

E

Q

H

N

R

K

D

D

A

D

L

L

A

A

A

R

L

I

T

T

R

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

R

H

G

G

E

E

V

I

G

L

Y
|
Y

A

S

A

A

S

F

F

F

F

L

E

E

W

W

F

F

A

S

E

E

A

A

K

R

R

R

A

V

Y

Y

G

G

D

D

V

I

I

I

P

H

S

T

P

P

N

A

P

K

N

D

A

R

K

R

I

A

I

L

V

V

T

L

R

K

E

Q

P

P

V

I

G

G

V

V

V

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F
|
F

P

P

L

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

V

V

L

V

K
|
K

P

P

S

A

E

E

E

D

T

T

P

P

L

F

S

S

A

A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

S

K

Q

A

A

G

G

I

I

P

P

P

S

G

G

V

V

F

Y

N

N

V

V

V

I

-

P

-

C

-

S

S

R

G

K

D

N

A

A

V

K

A

E

I

V

G

G

G

E

A

A

L

I

T

C

E

T

S

D

D

P

V

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S

S

T

T

R

T

V

T

G

G

K

K

L

I

L

L

A

L

K

H

Q

H

S

A

A

A

D

N

T

S

L

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

A

Q

A

A

V

V

Q

A

G

G

A

A

M

M

A

A

S

S

K

K

F

F

R
|
R

N

N

T

T

G

G

Q

Q

T

T

C
x
A

V
|
V

C

C

V

S

N

N

R

Q

F

F

Y

L

V

V

Q

Q

D

R

G

G

I

I

Y

H

D

D

A

A

F

F

T

V

L

K

A

A

L

F

A

A

Q

E

A

A

A

M

R

K

K

K

-

N

M

L

R

R

V

V

G

G

N

N

A

G

L

F

Q

E

G

E

D

G

V

T

E

T

Q

Q

G

G

P

P

L

L

I

I

N

N

Q

E

A

K

A

A

L

V

T

E

K

K

V

V

E

E

A

K

H

Q

V

V

A

N

D

D

A

A

L

V

Q

S

K

K

G

G

A

A

K

T

V

V

L

V

T

T

G

G

A

G

K

K

P

R

H

H

A

Q

L

L

G

G

G

K

T

N

F

F

Y

F

E

E

P

P

T

T

V

L

L

L

V

C

D

N

A

V

S

T

S

Q

S

D

M

M

L

L

I

C

A

T

Q

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

L

A

A

A

P

C

V

F

I

R

K

F

F

K

D

T

T

E

E

D

E

E

E

A

A

V

I

A

A

A

I

A

A

N

N

A

A

T

A

P

D

F

V

G

G

L

L

S

A

A

G

Y

Y

F

F

Y

Y

T

S

R

Q

D

D

L

P

A

A

R

Q

A

I

W

W

R

R

V

V

G

A

E

E

A

Q

L

L

E

E

S

V

G

G

M

M

V

V

G

G

I

V

N

N

E

E

G

G

I

L

L

I

S
|
S

T

S

E

V

V

E

A

C

P

P

F
|
F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

L

I

D

D

E

E

Y

Y

T

L

E

E

L

L

K

K

Y

Y

M

V

M

C

M

Y

G

G

L

L

active site: N155 (= N151), K178 (= K174), E256 (= E249), A290 (≠ C283), E388 (= E380), E465 (= E457)
binding succinic acid: Y109 (= Y105), F156 (= F152), R163 (= R159), E256 (= E249), R284 (= R277), A290 (≠ C283), V291 (= V284), S448 (= S440), F454 (= F446)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
43% identity, 98% coverage: 4:474/479 of query aligns to 2:474/476 of 5x5uA

query
sites
5x5uA

F

Y

L

T

R

D

T

T

G

Q

H

L

Y

L

I

I

G

D

G

G

E

E

W

W

Y

V

E
x
D

G
x
A

A
|
A

S

S

-
x
G

-

K

T

T

Y

I

P

D

V

V

L

V

N

N

P

P

A

A

T

T

G

G

E

K

V

P

I

I

A

G

Q

R

V

V

A

A

K

H

G

A

G

G

A

I

V

A

E

D

A

L

T

D

Q

R

A

A

I

L

A

A

E

Q

R

S

A

G

F

F

P

E

A

A

W

W

R

R

S

K

L

V

T

P

A

A

K

H

E

E

R

R

S

A

A

A

R

T

V

M

K

R

R

K

W

A

G

A

E

A

L

L

M

V

L

R

E

E

H

R

R

A

D

D

A

A

L

I

A

A

A

Q

L

L

L

M

T

T

R

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

R

R

G

V

E

E

V

V

G

L

Y

S

A

A

S

D

F

I

E

E

W

W

F

F

A

A

E

D

E

E

A

G

K

R

R

R

A

V

Y

Y

G

G

D

R

V

I

I

V

P

P

S

P

P

R

N

N

P

L

N

G

A

A

K

Q

I

Q

I

T

V

V

T

V

R

K

E

E

P

P

V

V

G

G

V

P

V

V

A

A

I

F

T

T

P
|
P

W
|
W

N
|
N

F

F

P

P

L

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

G

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

V

L

L

V

K
|
K

P

A

S

P

E

E

T

T

P

P

L

A

S

S

A

P

L

A

A

A

L

L

A

L

V

R

L

A

A

F

E

V

K

D

A

A

G

G

I

V

P

P

P

A

G

G

V

V

F

I

N

G

V

L

V

V

S

Y

G

G

D

D

A
x
P

V
x
A

A

E

I

I

G
x
S

G

S

A

Y

L

L

T

I

E

P

S

H

D

P

V

V

V

I

R

R

K

K

L

V

S

T

F
|
F

T
|
T

G
|
G

S
|
S

T

T

R

P

V
|
V

G

G

K

K

L

Q

L
|
L

A

A

K

S

Q

L

S

A

A

G

D

L

T

H

L

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L
|
L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

L

V

D

A

A

L

A

A

V

V

Q

K

G

A

A

A

M

G

A

G

S

A

K

K

F

F

R

R

N

N

T

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

V

P

N

T

R

R

F

F

Y

L

V

V

Q

H

D

N

G

S

I

I

Y

R

D

D

A

E

F

F

T

T

L

R

A

A

L

L

A

V

Q

K

A

H

A

A

R

E

K

G

M

L

R

K

V

V

G

G

N

N

A

G

L

L

Q

E

G

E

D

G

V

T

E

T

Q

L

G

G

P

A

L

L

I

A

N

N

Q

P

A
x
R

L

L

T

T

K

A

V

M

E

A

A

S

H

V

V

I

A

D

D

N

A

A

L

R

Q

K

K

V

G

G

A

A

K

S

V

I

L

E

T

T

G

G

A

G

K

E

P

R

H

I

A

G

L

S

G

E

G

G

T

N

F

F

Y

F

E

A

P

P

T

T

V

V

L

I

V

A

D

N

A

V

S

P

S

L

S

D

M

A

L

D

I

V

A

F

Q

N

E

N

E
|
E

T

P

F
|
F

G

G

P

P

V

V

A

A

C

I

F

R

R

G

F

F

K

D

T

K

E

L

D

E

E

E

A

A

V

I

A

A

A

E

A

A

N

N

A

R

T

L

P

P

F

F

G

G

L

L

S

A

A

G

Y

Y

F

A

Y

F

T

T

R

R

D

S

L

F

A

A

R

N

A

V

W

H

R

L

V

L

G

T

E

Q

A

R

L

L

E

E

S

V

G

G

M

M

V

L

G

W

I

I

N

N

E

Q

G

P

I

A

L

T

S

P

T

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L

Y

G

G

R

S

E
|
E

G

G

S

G

K

P

Y

E

G

A

L

L

D

E

E

P

Y

Y

T

L

E

V

L

T

K

K

Y

S

M

V

M

T

M

V

active site: N151 (= N151), K174 (= K174), E249 (= E249), C283 (= C283), E380 (= E380), E457 (= E457)
binding glycerol: D15 (≠ E17), A16 (≠ G18), A17 (= A19), G19 (vs. gap)
binding nicotinamide-adenine-dinucleotide: P149 (= P149), W150 (= W150), N151 (= N151), P207 (≠ A207), A208 (≠ V208), S211 (≠ G211), F225 (= F225), T226 (= T226), G227 (= G227), S228 (= S228), V231 (= V231), L235 (= L235), E249 (= E249), L250 (= L250), C283 (= C283), R329 (≠ A329), R330 (≠ A330), E380 (= E380), F382 (= F382)

5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
43% identity, 98% coverage: 4:474/479 of query aligns to 2:474/476 of 5x5tA

query
sites
5x5tA

F

Y

L

T

R

D

T

T

G

Q

H

L

Y

L

I

I

G

D

G

G

E

E

W

W

Y

V

E

D

G

A

A

A

S

S

-

G

-

K

T

T

Y

I

P

D

V

V

L

V

N

N

P

P

A

A

T

T

G

G

E

K

V

P

I

I

A

G

Q

R

V

V

A

A

K

H

G

A

G

G

A

I

V

A

E

D

A

L

T

D

Q

R

A

A

I

L

A

A

E

Q

R

S

A

G

F

F

P

E

A

A

W

W

R

R

S

K

L

V

T

P

A

A

K

H

E

E

R

R

S

A

A

A

R

T

V

M

K

R

R

K

W

A

G

A

E

A

L

L

M

V

L

R

E

E

H

R

R

A

D

D

A

A

L

I

A

A

A

Q

L

L

L

M

T

T

R

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

R

R

G

V

E

E

V

V

G

L

Y

S

A

A

S

D

F

I

E

E

W

W

F

F

A

A

E

D

E

E

A

G

K

R

R

R

A

V

Y

Y

G

G

D

R

V

I

I

V

P

P

S

P

P

R

N

N

P

L

N

G

A

A

K

Q

I

Q

I

T

V

V

T

V

R

K

E

E

P

P

V

V

G

G

V

P

V

V

A

A

I

F

T

T

P

P

W

W

N
|
N

F

F

P

P

L

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

G

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

V

L

L

V

K
|
K

P

A

S

P

E

E

T

T

P

P

L

A

S

S

A

P

L

A

A

A

L

L

A

L

V

R

L

A

A

F

E

V

K

D

A

A

G

G

I

V

P

P

P

A

G

G

V

V

F

I

N

G

V

L

V

V

S

Y

G

G

D

D

A

P

V

A

A

E

I

I

G

S

A

Y

L

L

T

I

E

P

S

H

D

P

V

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G

G

S

S

T

T

R

P

V

V

G

G

K

K

L

Q

L

L

A
|
A

K

S

Q

L

S
x
A

A
x
G

D

L

T

H

L

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

L

V

D

A

A

L

A

A

V

V

Q

K

G

A

A

A

M

G

A

G

S

A

K

K

F

F

R

R

N

N

T

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

V

P

N

T

R

R

F

F

Y

L

V

V

Q

H

D

N

G

S

I

I

Y

R

D

D

A

E

F

F

T

T

L

R

A

A

L

L

A

V

Q

K

A

H

A

A

R

E

K

G

M

L

R

K

V

V

G

G

N

N

A

G

L

L

Q

E

G

E

D

G

V

T

E

T

Q

L

G

G

P

A

L

L

I

A

N

N

Q

P

A

R

L

L

T

T

K

A

V

M

E

A

A

S

H

V

V

I

A

D

D

N

A

A

L

R

Q

K

K

V

G

G

A

A

K

S

V

I

L

E

T

T

G

G

A

G

K

E

P

R

H

I

A

G

L

S

G

E

G

G

T

N

F

F

Y

F

E

A

P

P

T

T

V

V

L

I

V

A

D

N

A

V

S

P

S

L

S

D

M

A

L

D

I

V

A

F

Q

N

E

N

E
|
E

T

P

F

F

G

G

P

P

V

V

A

A

C

I

F

R

R

G

F

F

K

D

T

K

E

L

D

E

E

E

A

A

V

I

A

A

A

E

A

A

N

N

A

R

T

L

P

P

F

F

G

G

L

L

S

A

A

G

Y

Y

F

A

Y

F

T

T

R

R

D

S

L

F

A

A

R

N

A

V

W

H

R

L

V

L

G

T

E

Q

A

R

L

L

E

E

S

V

G

G

M

M

V

L

G

W

I

I

N

N

E

Q

G

P

I

A

L

T

S

P

T

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L
x
Y

G

G

R

S

E
|
E

G

G

S

G

K

P

Y

E

G

A

L

L

D

E

E

P

Y

Y

T

L

E

V

L

T

K

K

Y

S

M

V

M

T

M

V

active site: N151 (= N151), K174 (= K174), E249 (= E249), C283 (= C283), E380 (= E380), E457 (= E457)
binding glycerol: A236 (= A236), A239 (≠ S239), G240 (≠ A240), Y454 (≠ L454)

4neaA 1.90 angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betb) from staphylococcus aureus in complex with NAD+ and bme-free cys289 (see paper)
39% identity, 98% coverage: 3:473/479 of query aligns to 14:492/505 of 4neaA

query
sites
4neaA

E

K

F

H

L

L

R

S

T

Q

G

R

H

Q

Y

Y

I

I

G

D

G

G

E

E

W

W

Y

V

E

E

G

S

A

A

S

N

-

K

-

N

T

T

Y

R

P

D

V

I

L

I

N

N

P

P

A

Y

T

N

G

Q

E

E

V

V

I

I

A

F

Q

T

V

V

A

S

K

E

G

G

G

T

A

K

V

E

E

D

A

A

T

E

Q

R

A

A

I

I

A

L

A

A

E

R

R

R

A

A

F

F

P

E

A

S

-

G

-

E

W

W

R

S

S

Q

L

E

T

T

A

A

K

E

E

T

R

R

S

G

A

K

R

K

V

V

K

R

R

A

W

I

G

A

E

D

L

K

M

I

L

K

E

E

H

H

R

R

D

E

A

A

L

L

A

A

A

R

L

L

L

E

T

T

R

L

E

D

Q

T

G

G

K

K

P

T

L

L

A

E

E

E

A

S

R

Y

G

A

E

D

V

M

G

D

Y

D

A

I

A

H

S

N

F

V

F

F

E

M

W

Y

F

F

A

A

E

G

E

L

A

A

K

D

R

K

A

D

Y

G

G

G

D

E

V

M

I

I

P

D

S

S

P

P

N

I

P

P

N

D

A

T

K

E

I

S

I

K

V

I

T

V

R

K

E

E

P

P

V

V

G

G

V

V

A

T

A

Q

I
|
I

T

T

P
|
P

W
|
W

N
|
N

F

Y

P

P

L

L

A

L

M

Q

I

A

T

S

R

W

K

K

A

I

G

A

P

P

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

L

V

V

L

M

K
|
K

P

P

S

S

E
|
E

E

I

T

T

P

P

L

L

S

T

A

T

L

I

A

R

L

V

A

F

V

E

L

L

A

M

E

E

K

E

A

V

G

G

I

F

P

P

P

K

G

G

V

T

F

I

N

N

V

L

V

I

S

L

G

G

D
x
A

A
x
G

V
x
S

A

E

I

V

G
|
G

G
x
D

A

V

L

M

T

S

E

G

S

H

D

K

V

E

V

V

R

D

K

L

L

V

S

S

F
|
F

T
|
T

G
|
G

S
x
G

T

I

R

E

V
x
T

G

G

K

K

L
x
H

L
x
I

A

M

K

K

Q

N

S

A

A

A

D

N

T

N

L

V

K

T

K

N

L

I

S

A

L

L

E
|
E

L

L

G
|
G

G

G

N

K

A

N

P

P

F

N

I

I

V

I

F

F

D

D

A

A

D

D

L

F

D

E

A

L

A

A

V

V

Q

D

G

Q

A

A

M

L

A

N

S

G

K

G

F

Y

R

F

N

H

T

A

G

G

Q

Q

T

V

C
|
C

V

S

C

A

V

G

N

S

R

R

F

I

Y

L

V

V

Q

Q

D

N

G

S

I

I

Y

K

D

D

A

K

F

F

T

E

L

Q

A

A

L

L

A

I

Q

D

A

R

A

V

R

K

K

K

M

I

R

K

V

L

G

G

N

N

A

G

L

F

Q

D

G

A

D

D

V

T

E

E

Q

M

G

G

P

P

L

V

I

I

N

S

Q

T

A

E

A
x
H

L

R

T

N

K

K

V

I

E

E

A

S

H

Y

V

M

A

D

D

V

A

A

L

K

Q

A

K

E

G

G

A

A

K

T

V

I

L

A

T

V

G

G

A

G

K

K

-

R

-

P

-

D

-

R

P

D

H

D

A

L

L

K

G

D

G

G

T

L

F

F

Y

F

E

E

P

P

T

T

V

V

L

I

V

T

D

N

A

C

S

D

S

T

S

S

M

M

L

R

I

I

A

V

Q

Q

E

E

E
|
E

T

V

F
|
F

G

G

P

P

V

V

A

V

A

T

C

V

F

E

R

G

F

F

K

E

T

T

E

E

D

Q

E

E

A

A

V

I

A

Q

A

L

A

A

N

N

A

D

T

S

P

I

F

Y

G

G

L

L

S

A

A

G

Y

A

F

V

Y

F

T

S

R

K

D

D

L

I

A

G

R

K

A

A

W

Q

R

R

V

V

G

A

E

N

A

K

L

L

E

K

S

L

G

G

M

T

V

V

G

W

I

I

N

N

E

D

G

F

I

H

L

P

S

Y

T

F

E

A

V

Q

A

A

P

P

F

W

G

G

V

Y

K

K

Q

Q

S

S

G

G

L

I

G

G

R

R

E
|
E

G

L

S

G

K

K

Y

E

G

G

L

L

D

E

E

E

Y

Y

T

L

E

V

L

S

K

K

Y

H

M

I

M

L

active site: N166 (= N151), K189 (= K174), E264 (= E249), C298 (= C283), E399 (= E380), E476 (= E457)
binding nicotinamide-adenine-dinucleotide: I162 (= I147), P164 (= P149), W165 (= W150), N166 (= N151), K189 (= K174), E192 (= E177), A221 (≠ D206), G222 (≠ A207), S223 (≠ V208), G226 (= G211), D227 (≠ G212), F240 (= F225), T241 (= T226), G242 (= G227), G243 (≠ S228), T246 (≠ V231), H249 (≠ L234), I250 (≠ L235), E264 (= E249), G266 (= G251), C298 (= C283), H345 (≠ A330), E399 (= E380), F401 (= F382)

6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
39% identity, 96% coverage: 10:470/479 of query aligns to 5:471/477 of 6j76A

query
sites
6j76A

Y

Y

I

V

G

G

E

E

W

W

Y

I

E

D

G

A

A

S

S

N

-

G

-

Q

T

T

Y

E

P

P

V

V

L

V

N

S

P

P

A

I

T

N

G

E

E

E

V

V

I

L

A

A

Q

Y

V

I

A

Q

K

D

G

A

G

D

A

A

V

S

E

D

A

A

T

E

Q

R

A

V

I

L

A

A

A

V

A

A

E

T

R

T

A

A

F

Q

P

K

A

E

W

W

R

A

S

K

L

Q

T

P

A

A

K

R

E

Q

R

R

S

A

A

E

R

V

V

L

K

R

R

K

W

F

G

A

E

Q

L

L

M

I

L

R

E

D

H

N

R

K

D

Q

A

Y

L

L

A

A

A

E

L

L

T

V

R

K

E

E

Q

Q

G

G

K

K

P

L

L

L

A

K

E

V

A

A

R

L

G

G

E

E

V

V

G

E

Y

A

A

T

A

S

S

T

F

F

F

I

E

E

W

Y

F

A

A

C

E

D

E

W

A

A

K

R

R

Q

A

M

Y

D

G

G

D

D

V

I

I

V

P

K

S

S

P

D

N

N

P

A

N

N

A

E

K

Q

I

I

I

M

V

I

T

H

R

K

E

I

P

P

V

R

G

G

V

V

A

V

A

A

I
|
I

T
|
T

P
x
A

W
|
W

N
|
N

F

F

P

P

L

L

A

A

M

L

I

A

T

G

R

R

K

K

A

I

G

G

P

P

A

A

L

L

A

V

A

A

G

G

C

N

T

S

M

I

V

V

L

V

K
|
K

P
|
P

S
x
T

E
x
S

E

E

T

T

P

P

L

L

S

A

A

T

L

L

A

E

L

L

A

G

V

Y

L

L

A

A

E

E

K

Q

A

A

G

G

I

I

P

P

P

A

G

G

V

V

F

L

N

N

V

I

V

V

S

T

G

G

D
x
G

A
x
G

V
x
R

A

T

I

L

G
|
G

G
x
N

A

E

L

L

T

V

E

G

S

H

D

R

V

M

V

T

R

N

K

M

L

V

S

S

F

M

T
|
T

G
|
G

S
|
S

T

T

R

P

V
x
A

G

G

K

Q

L
x
S

L
x
I

A

I

K

R

Q

A

S

S

A

A

D

N

T

N

L

M

K

A

K

H

L

V

S

Q

L

L

E
|
E

L
|
L

G
|
G

G

G

N

K

A

A

P

P

F

F

I

I

V

V

F

M

D

E

D

D

A

A

D

D

L

L

D

E

A

Q

A

A

V

A

Q

A

G

A

A

A

M

L

A

H

S

S

K

R

F

F

R

D

N

N

T

C

G

G

Q

Q

T

V

C
|
C

V

T

C

C

V

N

N

E

R

R

F

M

Y

Y

V

V

Q

H

D

G

G

A

I

V

Y

Y

D

D

A

E

F

F

T

M

L

R

A

I

L

F

A

M

Q

G

A

K

A

V

R

E

K

A

M

I

R

K

V

V

G

G

N

D

A

P

L

M

Q

D

G

P

D

A

V

S

E

D

Q

M

G

G

P

P

L

K

I

V

N

N

Q

A

A

N

A

E

L

L

T

A

K

H

V

M

E

E

A

E

H

L

V

V

A

A

D

E

A

A

L

V

Q

D

K

E

G

G

A

A

K

T

V

V

L

L

T

F

G

G

A

G

K

K

-

K

-

L

-

E

-

G

P

P

H

E

A

F

L

E

G

K

G

G

T

F

F

W

Y

F

E

E

P

P

T

T

V

V

L

L

V

T

D

N

A

V

S

T

S

Q

S

D

M

M

L

T

I

I

A

V

Q

H

E

E

E
|
E

T

S

F
|
F

G

G

P

P

V

I

A

L

A

P

C

V

F

I

R

K

F

F

K

D

T

S

E

F

D

D

E

E

A

V

V

I

A

E

A

Y

A

A

N

N

A

D

T

S

P

D

F

Y

G

G

L

L

S

A

A

A

Y

M

F

I

Y

C

T

T

R

Q

D

N

L

M

A

H

R

Y

A

I

W

N

R

R

V

L

G

L

E

T

A

E

L

L

E

E

S

S

G

G

M

E

V

I

G

Y

I

V

N

N

E

R

G

G

I

H

L

G

S

E

T

Q

E

H

V

Q

A

G

P

F

F
x
H

G

N

G

G

V

Y

K

K

Q

L

S

S

G

G

L

T

G

G

R

G

E
|
E

G

D

S

G

K

K

Y

Y

G

G

L

F

D

E

E

Q

Y

Y

T

L

E

E

L

K

K

K

active site: N148 (= N151), E246 (= E249), C280 (= C283), E458 (= E457)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I144 (= I147), T145 (= T148), A146 (≠ P149), W147 (= W150), N148 (= N151), K171 (= K174), P172 (= P175), T173 (≠ S176), S174 (≠ E177), G203 (≠ D206), G204 (≠ A207), R205 (≠ V208), G208 (= G211), N209 (≠ G212), T223 (= T226), G224 (= G227), S225 (= S228), A228 (≠ V231), S231 (≠ L234), I232 (≠ L235), E246 (= E249), L247 (= L250), G248 (= G251), C280 (= C283), E381 (= E380), F383 (= F382), H447 (≠ F446)

7radA Crystal structure analysis of aldh1b1
40% identity, 97% coverage: 10:474/479 of query aligns to 16:486/493 of 7radA

query
sites
7radA

Y

F

I

I

G

N

E

E

W

W

Y

Q

E

D

G

A

A

V

S

S

-

K

T

T

Y

F

P

P

V

T

L

V

N

N

P

P

A

T

T

T

G

G

E

E

V

V

I

I

A

G

Q

H

V

V

A

A

K

E

G

G

G

D

A

R

V

A

E

D

A

V

T

D

Q

R

A

A

I

V

A

K

A

A

E

R

R

E

A

A

F

F

-

R

-

L

-

G

P

S

A

P

W

W

R

R

S

R

L

M

T

D

A

A

K

S

E

E

R

R

S

G

A

R

R

L

V

L

K

N

R

R

W

L

G

A

E

D

L

L

M

V

L

E

E

R

H

D

R

R

D

V

A

Y

L

L

A

A

A

S

L

L

L

E

T

T

R

L

E

D

Q

N

G

G

K

K

P

P