SitesBLAST

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
63% identity, 98% coverage: 5:298/300 of query aligns to 3:292/303 of P16703

query
sites
P16703

T

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N71 (= N77) binding pyridoxal 5'-phosphate
S255 (= S261) binding pyridoxal 5'-phosphate

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
46% identity, 99% coverage: 3:298/300 of query aligns to 5:306/310 of 5xoqA

query
sites
5xoqA

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binding : T72 (= T74), S73 (= S75), G74 (= G76), T76 (= T78), M123 (= M125), Q144 (= Q146), R218 (vs. gap), H219 (vs. gap), Q222 (≠ R216), G223 (≠ K217), A226 (≠ E220)

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
44% identity, 98% coverage: 6:298/300 of query aligns to 7:305/306 of 2q3dA

query
sites
2q3dA

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active site: K44 (= K47), S266 (= S261), P293 (≠ C286)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K47), T71 (= T74), S72 (= S75), N74 (= N77), T75 (= T78), Q144 (= Q146), V177 (≠ M179), G178 (= G180), T179 (= T181), G180 (≠ T182), T182 (= T184), G222 (vs. gap), I223 (vs. gap), S266 (= S261), P293 (≠ C286), D294 (= D287)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 98% coverage: 6:298/300 of query aligns to 7:305/310 of P9WP55

query
sites
P9WP55

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K44 (= K47) modified: N6-(pyridoxal phosphate)lysine
N74 (= N77) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTTGT 180:184) binding pyridoxal 5'-phosphate
S266 (= S261) binding pyridoxal 5'-phosphate

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
43% identity, 99% coverage: 4:300/300 of query aligns to 7:310/322 of P47998

query
sites
P47998

K

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I

V

E

T

D

E

T

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

L

L

V

N

R

N

L

V

P

A

D

E

D

G

E

C

I

V

R

G

S

R

R

-

N

-

V

-

V

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

K

S

K

D

A

A

E

E

A

K

R

K

G

G

R

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

S

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

R

K

G

G

Y

Y

K

K

M

L

V

I

L

I

I

T

M

M

P

P

E

A

D

S

L

M

S

S

V

T

E

E

R

R

Q

I

S

I

M

L

A

L

A

A

Y

F

G

G

A

V

Q

E

I

L

I

V

L

L

T

T

P

D

V

P

T

A

G

K

G

G

M

M

E

K

Y

G

A

A

R

I

D

A

L

K

A

A

E

E

Q

E

-

I

M

L

Q

A

R

K

D

T

G

P

K

N

G

G

I

Y

I

M

L

L

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

A

K

A

I

H
|
H

V

Y

E

E

G

T

T

T

G
|
G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

E

G

G

G

R

K

I

I

T

D

H

G

F

F

V

V

S

S

S

G

M

I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

M

T

G

G

V

A

S

G

S

K

Y

Y

L

L

K

K

Q

E

Q

Q

N

N

Q

A

A

N

I

V

E

K

I

L

V

Y

G

G

A

V

Q

E

P

P

E

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R
x
K

K

P

W

G

P

P

E
x
H

-
x
K

-

I

-

Q

-

G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

S

I

V

F

L

D

N

R

V

S

D

R

L

V

I

D

D

R

E

V

V

E

V

N

Q

V

V

S

S

Q

S

A

D

A

E

A

S

E

I

A

D

M

M

A

A

R

R

R

Q

L

L

A

A

S

L

V

K

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

S

S

G

G

G

A

A

A

C

A

E

A

V

A

A

A

L

I

R

K

V

L

A

A

R

Q

Q

R

V

P

E

E

N

N

A

A

T

G

I

K

V

L

F

F

-

V

-

A

I

I

V

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

K46 (= K47) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T74) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S75) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N77) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T78) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q146) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H156) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G161) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTTGT 180:184) binding pyridoxal 5'-phosphate
T182 (= T181) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T184) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ R216) mutation to A: Impaired interaction with SAT1.
H221 (≠ E220) mutation to A: Impaired interaction with SAT1.
K222 (vs. gap) mutation to A: Impaired interaction with SAT1.
S269 (= S261) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
43% identity, 99% coverage: 4:300/300 of query aligns to 5:308/320 of 2isqA

query
sites
2isqA

K

K

T

D

I

V

E

T

D

E

T

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

L

L

V

N

R

N

L

V

P

A

D

E

D

G

E

C

I

V

R

G

S

R

R

-

N

-

V

-

V

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

K

S

K

D

A

A

E

E

A

K

R

K

G

G

R

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

S

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

R

K

G

G

Y

Y

K

K

M

L

V

I

L

I

I

T

M

M

P

P

E

A

D

S

L

M

S

S

V

T

E

E

R

R

Q

I

S

I

M

L

A

L

A

A

Y

F

G

G

A

V

Q

E

I

L

I

V

L

L

T

T

P

D

V

P

T

A

G

K

G
|
G

M
|
M

E
x
K

Y

G

A

A

R

I

D

A

L

K

A

A

E

E

Q

E

-

I

M

L

Q

A

R

K

D

T

G

P

K

N

G

G

I

Y

I

M

L

L

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

A

K

A

I

H

H

V

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

E

G

G

G

R

K

I

I

T

D

H

G

F

F

V

V

S

S

S
x
G

M

I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

V

A

S

G

S

K

Y

Y

L

L

K

K

Q

E

Q

Q

N

N

Q

A

A

N

I

V

E

K

I

L

V

Y

G

G

A

V

Q

E

P

P

E

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R

K

K

P

W
x
G

P
|
P

E
x
H

-

K

-

I

-
x
Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

S

I

V

F

L

D

N

R

V

S

D

R

L

V

I

D

D

R

E

V

V

E

V

N

Q

V

V

S

S

Q

S

A

D

A

E

A

S

E

I

A

D

M

M

A

A

R

R

R

Q

L

L

A

A

S

L

V

K

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

S

S

G

G

G

A

A

A

C

A

E

A

V

A

A

A

L

I

R

K

V

L

A

A

R

Q

Q

R

V

P

E

E

N

N

A

A

T

G

I

K

V

L

F

F

-

V

-

A

I

I

V

F

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

active site: K44 (= K47), S267 (= S261)
binding pyridoxal-5'-phosphate: K44 (= K47), N75 (= N77), G177 (≠ S178), G179 (= G180), T180 (= T181), G181 (≠ T182), T183 (= T184), G223 (vs. gap), S267 (= S261), P294 (≠ C286)
binding : T72 (= T74), S73 (= S75), G74 (= G76), T76 (= T78), G122 (= G124), M123 (= M125), K124 (≠ E126), G217 (≠ W218), P218 (= P219), H219 (≠ E220), Q222 (vs. gap), G223 (vs. gap)

P9WP53 O-phosphoserine sulfhydrylase; OPS sulfhydrylase; CysO-thiocarboxylate-dependent cysteine synthase; Cysteine synthase B; CSase B; O-phosphoserine-specific cysteine synthase; [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase; EC 2.5.1.113 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
42% identity, 99% coverage: 3:298/300 of query aligns to 4:306/323 of P9WP53

query
sites
P9WP53

Y

Y

K

D

T

S

I

L

E

L

D

Q

T

A

I

L

G

G

N

N

T

T

P

P

L

L

V

V

Q

G

L

L

V

Q

R

R

L

L

P

S

D

P

-

R

-

W

D

D

E

D

I

G

R

R

S

D

R

G

N

P

N

H

V

V

V

R

L

L

-

W

A

A

K

K

L

L

E

E

G

D

N

R

N

N

P

P

A

T

G

G

S

S

V

I

K

K

D

D

R

R

P

P

A

A

L

V

S

R

M

M

I

I

K

E

K

Q

A

A

E

E

A

A

R

D

G

G

R

L

I

L

K

R

P

P

G

G

D

A

T

T

L

I

I

L

E

E

S

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

S

L

L

A

A

M

M

A

A

A

R

I

L

R

K

G

G

Y

Y

K

R

M

L

V

I

L

C

I

V

M

M

P

P

E

E

D

N

L

T

S

S

V

V

E

E

R

R

Q

Q

S

L

M

L

A

E

A

L

Y

Y

G

G

A

A

Q

Q

I

I

L

F

T

S

P

A

V

A

T

E

G

G

M

S

E

N

Y

T

A

A

R

V

D

A

L

T

A

A

E

K

Q

E

M

L

-

A

Q

A

R

T

D

N

G

P

K

S

G

W

I

V

I

M

L

L

D

Y

Q

Q

F

Y

A

G

N

N

P

P

D

A

N

N

P

T

A

D

A

S

H

H

V

Y

E

C

G

G

T

T

G

G

P

P

E

E

I

L

W

L

R

A

D

D

T

L

E

P

G

-

R

E

I

I

T

T

H

H

F

F

V

V

S

A

S

G

M

L

G

G

T

T

G

G

T

T

I

L

M

M

G

G

V

T

S

G

S

R

Y

F

L

L

K

R

Q

E

Q

H

N

V

Q

A

A

N

I

V

E

K

I

I

V

V

G

A

A

A

Q

E

P

P

E

R

E

Y

G

G

S

E

R

G

I

V

P

Y

G

A

I

L

R
|
R

K

N

W

M

P

D

E

E

A

G

Y

F

M

V

P

P

K

E

I

L

F

Y

D

D

R

P

S

E

R

I

V

L

D

T

R

A

V

R

E

Y

N

S

V

V

S

G

Q

A

A

V

A

D

A

A

E

V

A

R

M

R

A

T

R

R

R

E

L

L

A

V

S

H

V

T

E

E

G

G

I

I

F

F

A

A

G

G

I

I

S
|
S

S

T

G

G

G

A

A

V

C

L

E

H

-

A

-

L

-

G

V

V

A

G

L

A

R

G

V

A

A

L

R

A

Q

A

V

G

E

E

N

R

A

A

T

D

I

I

V

A

F

L

I

V

V

V

C

A

D

D

R

A

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

G

V

A

F

Y

N81 (= N77) binding pyridoxal 5'-phosphate
R220 (= R216) mutation to A: 700-fold decrease in the rate of the first half-reaction using OPS. Affects neither the rate of the first half-reaction using OAS nor the rate of the second half-reaction using sulfide or CysO-COSH.
S265 (= S261) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

319:323 mutation Missing: Decreased lifetime of the alpha-aminoacrylate reaction intermediate, increased susceptibility to oxidation by oxidative agents such as hydrogen peroxide, and partial loss of selectivity towards CysO-COSH as sulfur donor.

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
44% identity, 96% coverage: 6:293/300 of query aligns to 7:300/300 of 3zeiA

query
sites
3zeiA

I

I

E

T

D

Q

T

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

Q

R

L

L

V

R

R

R

L

V

P

T

D

D

D

G

E

A

I

V

R

A

S

D

R

-

N

-

V

-

V

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

K

Q

K

A

A

A

E

E

A

Q

R

A

G

G

R

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

S

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

R

R

G

G

Y

Y

K

R

M

C

V

V

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

V

L

E

E

R

R

Q

M

S

L

M

L

A

R

A

A

Y

Y

G

G

A

A

Q

E

I

L

I

I

L

L

T

T

P

P

V

G

T

A

G

D

G

G

M

M

E

S

Y

G

A

A

R
x
I

D

A

L

K

A

A

E

E

Q

E

M

L

Q

A

R

K

-

T

D

D

G

Q

K

R

G

Y

I

F

I

V

L

P

D

Q

Q
|
Q

F
|
F

A

E

N

N

P

P

D

A

N

N

P

P

A

A

A

I

H

H

V

R

E

V

G

T

T

T

G

A

P

E

E

E

I

V

W

W

R

R

D

D

T

T

E

D

G

G

R

K

I

V

T

D

H

I

F

V

V

V

S

A

S

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

V

V

S

A

S

Q

Y

V

L

I

K

K

Q

E

Q

R

N

K

Q

P

A

S

I

A

E

R

I

F

V

V

G

A

A

V

Q

E

P

P

E

A

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K
|
K

W

G

P

P

E

H

-

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

A

G

Y
x
F

M

V

P

P

K

P

I

V

F

L

D

D

R

Q

S

D

R

L

V

V

D

D

R

E

V

I

E

I

N

T

V

V

S

G

Q

N

A

E

A

D

A

A

E

L

A

N

M

V

A

A

R

R

L

L

A

A

S

R

V

E

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

S

S

G

G

G

A

A

A

C

T

E

V

V

A

A

A

L

L

R

Q

V

V

A

A

R

R

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

T

L

I

I

V

V

F

V

I

V

V

L

C
x
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K47), S266 (= S261), P293 (≠ C286)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T74), S72 (= S75), I126 (≠ R129), Q144 (= Q146), F145 (= F147), K215 (= K217), G222 (vs. gap), A225 (vs. gap), F227 (≠ Y222)
binding pyridoxal-5'-phosphate: K44 (= K47), N74 (= N77), V177 (≠ M179), G178 (= G180), T179 (= T181), G180 (≠ T182), T182 (= T184), G222 (vs. gap), S266 (= S261), P293 (≠ C286), D294 (= D287)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
44% identity, 96% coverage: 6:293/300 of query aligns to 7:300/300 of 2q3cA

query
sites
2q3cA

I

I

E

T

D

Q

T

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

Q

R

L

L

V

R

R

R

L

V

P

T

D

D

D

G

E

A

I

V

R

A

S

D

R

-

N

-

V

-

V

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

K

Q

K

A

A

A

E

E

A

Q

R

A

G

G

R

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

S

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

R

R

G

G

Y

Y

K

R

M

C

V

V

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

V

L

E

E

R

R

Q

M

S

L

M

L

A

R

A

A

Y

Y

G

G

A

A

Q

E

I

L

I

I

L

L

T

T

P

P

V

G

T

A

G

D

G

G

M
|
M

E

S

Y

G

A

A

R

I

D

A

L

K

A

A

E

E

Q

E

M

L

Q

A

R

K

-

T

D

D

G

Q

K

R

G

Y

I

F

I

V

L

P

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

A

A

A

I

H

H

V

R

E

V

G

T

T

T

G

A

P

E

E

E

I

V

W

W

R

R

D

D

T

T

E

D

G

G

R

K

I

V

T

D

H

I

F

V

V

V

S

A

S

G

M

V

G

G

T

T

T

G

G

G

T

T

I

I

M

T

G

G

V

V

S

A

S

Q

Y

V

L

I

K

K

Q

E

Q

R

N

K

Q

P

A

S

I

A

E

R

I

F

V

V

G

A

A

V

Q

E

P

P

E

A

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K
|
K

W

G

P

P

E

H

-

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

A

G

Y

F

M

V

P

P

K

P

I

V

F

L

D

D

R

Q

S

D

R

L

V

V

D

D

R

E

V

I

E

I

N

T

V

V

S

G

Q

N

A

E

A

D

A

A

E

L

A

N

M

V

A

A

R

R

L

L

A

A

S

R

V

E

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

S

S

G

G

G

A

A

A

C

T

E

V

V

A

A

A

L

L

R

Q

V

V

A

A

R

R

Q

R

V

P

E

E

N

N

A

A

-

G

-

K

T

L

I

I

V

V

F

V

I

V

V

L

C
x
P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K47), S266 (= S261), P293 (≠ C286)
binding : T71 (= T74), S72 (= S75), G73 (= G76), T75 (= T78), M122 (= M125), Q144 (= Q146), K215 (= K217), G222 (vs. gap), A225 (vs. gap)

3dwgA Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of mycobacterium tuberculosis (see paper)
42% identity, 99% coverage: 3:298/300 of query aligns to 6:308/325 of 3dwgA

query
sites
3dwgA

Y

Y

K

D

T

S

I

L

E

L

D

Q

T

A

I

L

G

G

N

N

T

T

P

P

L

L

V

V

Q

G

L

L

V

Q

R

R

L

L

P

S

D

P

-

R

-

W

D

D

E

D

I

G

R

R

S

D

R

G

N

P

N

H

V

V

V

R

L

L

-

W

A

A

K

K

L

L

E

E

G

D

N

R

N

N

P

P

A

T

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

V

S

R

M

M

I

I

K

E

K

Q

A

A

E

E

A

A

R

D

G

G

R

L

I

L

K

R

P

P

G

G

D

A

T

T

L

I

I

L

E

E

S

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

S

L

L

A

A

M

M

A

A

A

R

I

L

R

K

G

G

Y

Y

K

R

M

L

V

I

L

C

I

V

M

M

P

P

E

E

D

N

L

T

S

S

V

V

E

E

R

R

Q

Q

S

L

M

L

A

E

A

L

Y

Y

G

G

A

A

Q

Q

I

I

L

F

T

S

P

A

V

A

T

E

G

G

M

S

E

N

Y

T

A

A

R

V

D

A

L

T

A

A

E

K

Q

E

M

L

-

A

Q

A

R

T

D

N

G

P

K

S

G

W

I

V

I

M

L

L

D

Y

Q

Q

F

Y

A

G

N

N

P

P

D

A

N

N

P

T

A

D

A

S

H

H

V

Y

E

C

G

G

T

T

G

G

P

P

E

E

I

L

W

L

R

A

D

D

T

L

E

P

G

-

R

E

I

I

T

T

H

H

F

F

V

V

S

A

S

G

M

L

G
|
G

T
|
T

G
|
G

T
|
T

I

L

M

M

G

G

V

T

S

G

S

R

Y

F

L

L

K

R

Q

E

Q

H

N

V

Q

A

A

N

I

V

E

K

I

I

V

V

G

A

A

A

Q

E

P

P

E

R

E

Y

G

G

S

E

R

G

I

V

P

Y

G

A

I

L

R

R

K

N

W

M

P

D

E

E

A

G

Y

F

M

V

P

P

K

E

I

L

F

Y

D

D

R

P

S

E

R

I

V

L

D

T

R

A

V

R

E

Y

N

S

V

V

S

G

Q

A

A

V

A

D

A

A

E

V

A

R

M

R

A

T

R

R

R

E

L

L

A

V

S

H

V

T

E

E

G

G

I

I

F

F

A

A

G

G

I

I

S
|
S

S

T

G

G

G

A

A

V

C

L

E

H

-

A

-

L

-

G

V

V

A

G

L

A

R

G

V

A

A

L

R

A

Q

A

V

G

E

E

N

R

A

A

T

D

I

I

V

A

F

L

I

V

V

V

C
x
A

D
|
D

R

A

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

G

V

A

F

Y

active site: K53 (= K47), S267 (= S261)
binding pyridoxal-5'-phosphate: K53 (= K47), N83 (= N77), G186 (= G180), T187 (= T181), T188 (= T182), G189 (= G183), T190 (= T184), S267 (= S261), A296 (≠ C286), D297 (= D287)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
43% identity, 99% coverage: 4:300/300 of query aligns to 5:308/320 of 1z7yA

query
sites
1z7yA

K

K

T

D

I

V

E

T

D

E

T

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

L

L

V

N

R

N

L

V

P

A

D

E

D

G

E

C

I

V

R

G

S

R

R

-

N

-

V

-

V

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
x
A

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

K

S

K

D

A

A

E

E

A

K

R

K

G

G

R

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

S

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

R

K

G

G

Y

Y

K

K

M

L

V

I

L

I

I

T

M

M

P

P

E

A

D

S

L

M

S

S

V

T

E

E

R

R

Q

I

S

I

M

L

A

L

A

A

Y

F

G

G

A

V

Q

E

I

L

I

V

L

L

T

T

P

D

V

P

T

A

G

K

G

G

M

M

E

K

Y

G

A

A

R

I

D

A

L

K

A

A

E

E

Q

E

-

I

M

L

Q

A

R

K

D

T

G

P

K

N

G

G

I

Y

I

M

L

L

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

A

K

A

I

H

H

V

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

E

G

G

G

R

K

I

I

T

D

H

G

F

F

V

V

S

S

S

G

M
x
I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

V

A

S

G

S

K

Y

Y

L

L

K

K

Q

E

Q

Q

N

N

Q

A

A

N

I

V

E

K

I

L

V

Y

G

G

A

V

Q

E

P

P

E

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R

K

K

P

W

G

P

P

E

H

-

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

S

I

V

F

L

D

N

R

V

S

D

R

L

V

I

D

D

R

E

V

V

E

V

N

Q

V

V

S

S

Q

S

A

D

A

E

A

S

E

I

A

D

M

M

A

A

R

R

R

Q

L

L

A

A

S

L

V

K

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

S

S

G

G

G

A

A

A

C

A

E

A

V

A

A

A

L

I

R

K

V

L

A

A

R

Q

Q

R

V

P

E

E

N

N

A

A

T

G

I

K

V

L

F

F

-

V

-

A

I

I

V

F

C
x
P

D
x
S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

active site: A44 (≠ K47), S267 (= S261)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G76), N75 (= N77), T76 (= T78), Q145 (= Q146), I178 (≠ M179), G179 (= G180), T180 (= T181), G181 (≠ T182), T183 (= T184), G223 (vs. gap), S267 (= S261), P294 (≠ C286), S295 (≠ D287)

3bm5A Crystal structure of o-acetyl-serine sulfhydrylase from entamoeba histolytica in complex with cysteine (see paper)
41% identity, 99% coverage: 3:298/300 of query aligns to 13:318/338 of 3bm5A

query
sites
3bm5A

Y

Y

K

H

T

N

I

I

E

L

D

E

T

T

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

E

L

L

V

H

R

G

L

V

P

T

D

E

D

H

E

P

I

R

R

I

S

K

R

K

N

G

N

T

V

R

V

I

L

L

A

V

K

K

L

L

E

E

G

Y

N

F

N

N

P

P

A

M

G

S

S

S

V

V

K
|
K

D

D

R

R

P

V

A

G

L

F

S

N

M

I

I

V

K

Y

K

Q

A

A

E

I

A

K

R

D

G

G

R

R

I

L

K

K

P

P

G

G

D

M

T

E

L

I

I

I

E

E

S

S

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

C

M

Q

A

A

A

G

A

A

I

V

R

F

G

G

Y

Y

K

R

M

V

V

N

L

I

I

A

M

M

P

P

E

S

D

T

L

M

S

S

V

V

E

E

R

R

R

Q

Q

M

S

I

M

M

A

K

A

A

Y

F

G

G

A

A

Q

E

I

L

I

I

L

L

T

T

P

E

V

G

T

K

G

K

G

G

M

M

E

P

Y

G

A

A

R

I

D

E

L

E

A

V

E

N

Q

K

M

M

Q

I

R

K

D

E

-

N

-

P

G

G

K

K

G

Y

I

F

I

V

L

A

D

N

Q

Q

F

F

A

G

N

N

P

P

D

D

N

N

P

T

A

A

H

H

V

-

E

H

G

Y

T

T

G

A

P

N

E

E

I

I

W

W

R

E

D

D

T

T

E

D

G

G

R

E

I

V

T

D

H

I

F

V

V

V

S

S

S

A

M

V

G
|
G

T
|
T

T
x
S

G

G

T
|
T

I

V

M

I

G

G

V

V

S

A

S

E

Y

K

L

L

K

K

Q

E

Q

K

N

K

Q

K

A

G

I

I

E

K

I

I

V

I

G

A

A

V

Q

E

P

P

E

E

G

S

S

A

R

V

I

L

P

E

G

G

I

K

R

A

K

K

W

G

P

P

E

H

-

G

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

D

I

I

F

Y

D

K

R

K

S

E

R

F

V

V

D

D

R

E

V

I

E

I

N

P

V

I

S

K

Q

T

A

Q

A

D

A

A

E

W

A

K

M

M

A

A

R

R

R

A

L

V

A

V

S

K

V

Y

E

D

G

G

I

I

F

M

A

C

G

G

I

M

S
|
S

S

S

G

G

G

A

A

A

C

I

E

L

V

A

A

G

L

L

R

K

V

E

A

A

R

E

Q

K

V

P

E

E

N

N

-

E

-

G

A

K

T

T

I

I

V

V

F

I

I

I

V

V

C
x
P

D

S

R

C

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

D

V

L

F

Y

active site: K57 (= K47), S279 (= S261)
binding pyridoxal-5'-phosphate: K57 (= K47), N87 (= N77), G191 (= G180), T192 (= T181), S193 (≠ T182), T195 (= T184), G235 (vs. gap), S279 (= S261), P306 (≠ C286)

4jbnA Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with serine acetyl transferase derived tetrapeptide, spsi (see paper)
41% identity, 99% coverage: 3:298/300 of query aligns to 13:318/336 of 4jbnA

query
sites
4jbnA

Y

Y

K

H

T

N

I

I

E

L

D

E

T

T

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

E

L

L

V

H

R

G

L

V

P

T

D

E

D

H

E

P

I

R

R

I

S

K

R

K

N

G

N

T

V

R

V

I

L

L

A

V

K

K

L

L

E

E

G

Y

N

F

N

N

P

P

A

M

G

S

S

S

V

V

K
|
K

D

D

R

R

P

V

A

G

L

F

S

N

M

I

I

V

K

Y

K

Q

A

A

E

I

A

K

R

D

G

G

R

R

I

L

K

K

P

P

G

G

D

M

T

E

L

I

I

I

E

E

S

S

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

C

M

Q

A

A

A

G

A

A

I

V

R

F

G

G

Y

Y

K

R

M

V

V

N

L

I

I

A

M

M

P

P

E

S

D

T

L

M

S

S

V

V

E

E

R

R

R

Q

Q

M

S

I

M

M

A

K

A

A

Y

F

G

G

A

A

Q

E

I

L

I

I

L

L

T

T

P

E

V

G

T

K

G

K

G

G

M

M

E

P

Y

G

A

A

R

I

D

E

L

E

A

V

E

N

Q

K

M

M

Q

I

R

K

D

E

-

N

-

P

G

G

K

K

G

Y

I

F

I

V

L

A

D

N

Q
|
Q

F

F

A

G

N

N

P

P

D

D

N

N

P

T

A

A

H

H

V

-

E

H

G

Y

T

T

G

A

P

N

E

E

I

I

W

W

R

E

D

D

T

T

E

D

G

G

R

E

I

V

T

D

H

I

F

V

V

V

S

S

S

A

M

V

G
|
G

T

T

T

S

G

G

T

T

I

V

M

I

G

G

V

V

S

A

S

E

Y

K

L

L

K

K

Q

E

Q

K

N

K

Q

K

A

G

I

I

E

K

I

I

V

I

G

A

A

V

Q

E

P

P

E

E

G

S

S

A

R

V

I

L

P

E

G

G

I

K

R

A

K

K

W

G

P

P

E

H

-

G

-

I

-

Q

-

G

-

I

-

G

-

A

A

G

Y

F

M

I

P

P

K

D

I

I

F

Y

D

K

R

K

S

E

R

F

V

V

D

D

R

E

V

I

E

I

N

P

V

I

S

K

Q

T

A

Q

A

D

A

A

E

W

A

K

M

M

A

A

R

R

R

A

L

V

A

V

S

K

V

Y

E

D

G

G

I

I

F

M

A

C

G

G

I

M

S
|
S

S

S

G

G

G

A

A

A

C

I

E

L

V

A

A

G

L

L

R

K

V

E

A

A

R

E

Q

K

V

P

E

E

N

N

-

E

-

G

A

K

T

T

I

I

V

V

F

I

I

I

V

V

C

P

D

S

R

C

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

D

V

L

F

Y

active site: K57 (= K47), S279 (= S261)
binding : K57 (= K47), T84 (= T74), S85 (= S75), G86 (= G76), T88 (= T78), Q158 (= Q146), G191 (= G180)

4il5A Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with isoleucine (see paper)
41% identity, 99% coverage: 3:298/300 of query aligns to 13:318/336 of 4il5A

query
sites
4il5A

Y

Y

K

H

T

N

I

I

E

L

D

E

T

T

I

I

G

G

N

G

T

T

P

P

L

L

V

V

Q

E

L

L

V

H

R

G

L

V

P

T

D

E

D

H

E

P

I

R

R

I

S

K

R

K

N

G

N

T

V

R

V

I

L

L

A

V

K

K

L

L

E

E

G

Y

N

F

N

N

P

P

A

M

G

S

S

S

V

V

K
|
K

D

D

R

R

P

V

A

G

L

F

S

N

M

I

I

V

K

Y

K

Q

A

A

E

I

A

K

R

D

G

G

R

R

I

L

K

K

P

P

G

G

D

M

T

E

L

I

I

I

E

E

S

S

T

T

S

S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

C

M

Q

A

A

A

G

A

A

I

V

R

F

G

G

Y

Y

K

R

M

V

V

N

L

I

I

A

M

M

P

P

E

S

D

T

L

M

S

S

V

V

E

E

R

R

R

Q

Q

M

S

I

M

M

A

K

A

A

Y

F

G

G

A

A

Q

E

I

L

I

I

L

L

T

T

P

E

V

G

T

K

G

K

G

G

M

M

E

P

Y

G

A

A

R

I

D

E

L

E

A

V

E

N

Q

K

M

M

Q

I

R

K

D

E

-

N

-

P

G

G

K

K

G

Y

I

F

I

V

L

A

D

N

Q
|
Q

F
|
F

A

G

N

N

P

P

D

D

N

N

P

T

A

A

H

H

V

-

E

H

G

Y

T

T

G

A

P

N

E

E

I

I

W

W

R

E

D

D