SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_03925 H281DRAFT_03925 chorismate mutase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 18 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
34% identity, 98% coverage: 7:357/360 of query aligns to 5:376/386 of P0A9J8

query
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P0A9J8

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R11 (= R13) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R30) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K41) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E54) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (≠ E89) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

2qmxA The crystal structure of l-phe inhibited prephenate dehydratase from chlorobium tepidum tls (see paper)
38% identity, 73% coverage: 95:356/360 of query aligns to 5:273/278 of 2qmxA

query
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2qmxA

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active site: T169 (= T261), F171 (= F263)
binding phenylalanine: N204 (= N287), E205 (= E288), L209 (≠ V292), D222 (≠ S305), L223 (≠ M306), T224 (= T307), K225 (≠ R308), S228 (= S311), Y238 (= Y321), F240 (= F323)

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
35% identity, 74% coverage: 92:359/360 of query aligns to 1:277/306 of 3mwbA

query
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3mwbA

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active site: T176 (= T261), F178 (= F263)
binding phenylalanine: D205 (≠ E288), H206 (vs. gap), L210 (≠ V292), N223 (≠ S305), L224 (≠ M306), S225 (≠ T307), R226 (= R308), S229 (= S311), F241 (= F323)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
35% identity, 74% coverage: 92:359/360 of query aligns to 1:274/303 of 3mwbB

query
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3mwbB

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active site: T176 (= T261), F178 (= F263)
binding magnesium ion: A155 (= A240), Q158 (≠ H243), L161 (= L246)
binding phenylalanine: D205 (≠ N287), H206 (≠ E288), L210 (≠ V292), N223 (≠ S305), L224 (≠ M306), S225 (≠ T307), R226 (= R308), I227 (≠ F309), Y236 (= Y321), F238 (= F323)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
33% identity, 74% coverage: 90:356/360 of query aligns to 5:276/282 of 6vh5D

query
sites
6vh5D

K

K

T

T

I

N

K

R

A

I

A

S

Y

F

L

Q

G

G

P

E

V

A

G

G

T

A

Y

N

S

S

E

D

Q

T

A

A

M

C

H

R

E

N

Y

M

F

F

G

-

Q

P

S

D

I

M

E

E

G

P

L

L

P

P

C

C

P

P

S

T

I

F

D

E

E

D

V

A

F

F

R

N

S

A

V

V

E

E

A

T

G

G

A

A

E

D

F

L

G

A

V

M

V

I

P

P

V

I

E

E

N

N

S

T

T

L

E

A

G

G

A

R

V

V

S

A

R

D

T

I

L

H

D

Y

L

L

L

P

Q

L

T

A

Q

D

L

M

A

H

I

I

G

V

G

G

E

E

L

Y

A

F

L

L

P

P

I

I

H

H

H

F

N

Q

L

L

L

M

T

V

L

L

N

P

G

G

-

V

G

R

L

R

A

E

G

E

V

I

T

K

R

T

V

V

C

H

A

S

H

H

A

I

Q

H

A

A

L

L

A

G

Q

Q

C

C

Q

R

R

N

W

V

L

I

A

R

T

Q

N

N

A

G

P

-

H

-

L

W

E

K

R

G

Q

V

A

I

V

A

S

G

S

D

N

T

A

A

E

G

A

A

R

R

M

L

A

V

A

A

E

D

-

V

-

K

D

D

P

R

T

S

V

M

A

A

I

L

A

A

G

P

D

R

R

L

A

A

T

D

H

L

Y

Y

G

G

L

L

Q

D

V

I

A

L

Y

E

A

E

L

N

I

V

Q

E

D

D

D

S

P

E

H

N

N

N

R

V

T

T

R

R

F

F

V

V

M

V

I

L

G

S

K

K

E

N

R

K

T

Q

G

W

V

A

S

A

G

R

H

P

D

E

Q

N

-

D

-

E

-

R

-

I

-

V

T

T

S

T

L

F

I

V

V

F

S

R

V

V

A

R

N
|
N

E
x
V

P

P

G

A

A

A

V
x
L

F

Y

K

K

L

A

L

L

E

G

P

G

L

F

A

A

R

T

H

N

S

G

V

V

S
x
N

M
|
M

T
|
T

R
x
K

F

L

E

E

S
|
S

R

Y

P

Q

A

L

R

G

V

G

G

R

T

F

W

I

E

A

Y
x
T

Y

Q

F
|
F

Y

Y

I

A

D

D

V

I

E

E

G

G

H

H

R

P

D

E

P

R

A

S

V

V

A

Q

A

L

A

A

L

L

E

E

L

L

G

R

Q

F

K

F

A

T

A

K

F

E

L

V

K

R

I

I

L

L

G

G

S

V

Y

Y

binding phenylalanine: N207 (= N287), V208 (≠ E288), L212 (≠ V292), N225 (≠ S305), M226 (= M306), T227 (= T307), K228 (≠ R308), S231 (= S311), T241 (≠ Y321), F243 (= F323)

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
33% identity, 73% coverage: 95:357/360 of query aligns to 5:270/278 of 7am0B

query
sites
7am0B

A

A

Y

F

L

Q

G

G

P

R

V

P

G

G

T

A

Y

Y

S

S

E

D

Q

L

A

A

M

C

H

R

E

-

Y

-

F

-

G

-

Q

Q

S

A

I

R

E

P

G

G

-

W

-

T

L

L

P

P

C

C

P

Q

S

T

I

F

D

A

E

Q

V

T

F

I

R

A

S

A

V

V

E

H

A

D

G

G

A

R

A

A

E

E

F

L

G

A

V

M

V

L

P

A

V

C

E

E

N

N

S

S

T

L

E

A

G

G

A

R

V

V

S

P

R

D

T

I

L

H

D

A

L

L

L

P

Q

E

T

A

Q

G

L

L

A

F

I

I

G

V

G

G

E

E

L

H

A

F

L

Q

P

R

I

V

H

E

H

H

N

C

L

L

T

G

L

I

N

P

G

G

G

S

-

T

L

L

A

A

G

D

V

A

T

R

R

R

V

I

C

H

A

T

H

H

A

P

Q

V

A

A

L

M

A

A

Q

Q

C

V

Q

-

R

R

W

G

L

I

A

I

T

T

N

E

A

L

P

-

H

G

L

L

E

D

R

P

Q

V

A

V

V

E

S

F

S

D

N

T

A

A

E

G

A

A

R

E

M

M

A

V

A

R

E

E

-

W

-

G

D

R

P

K

T

E

V

D

A

V

A

A

I

V

A

A

G

S

D

A

R

L

A

A

T

E

H

L

Y

N

G

G

L

L

Q

E

V

I

A

L

Y

R

A

R

L

N

I

V

Q

E

D

D

D

A

P

T

H

H

N

N

R

T

T

T

R

R

F

F

V

Y

M

I

I

A

G

S

K

R

E

R

R

P

T

A

G

T

V

L

S

P

G

P

H

P

D

G

-

P

-

G

-

F

Q

M

T

T

S

T

L

L

I

L

V

F

S

R

V

V

A
x
N

N
|
N

E
x
Q

P
|
P

G

G

A

A

V
x
L

F

Y

K

K

L

A

L

L

E

G

P

G

L

L

A

A

R

T

H

A

S

G

V

V

S
x
N

M
|
M

T
|
T

R
|
R

F

L

E

E

S

S

R

Y

P

M

A

L

R

E

V

G

G

S

T

F

W

S

E

A

Y
x
T

Y

Q

F
|
F

Y

L

I

M

D

D

V

V

E

E

G

G

H

H

R

P

D

E

D

A

P

P

A

P

V

L

A

A

A

R

A

A

L

L

E

D

E

E

L

L

G

S

Q

F

K

F

A

S

A

E

F

Q

L

Q

K

E

I

I

L

L

G

G

S

V

Y

Y

P

P

binding phenylalanine: N199 (≠ A286), N200 (= N287), Q201 (≠ E288), P202 (= P289), L205 (≠ V292), N218 (≠ S305), M219 (= M306), T220 (= T307), R221 (= R308), T234 (≠ Y321), F236 (= F323)

3luyA Putative chorismate mutase from bifidobacterium adolescentis
26% identity, 74% coverage: 93:358/360 of query aligns to 5:285/326 of 3luyA

query
sites
3luyA

K

K

A

L

A

F

Y

Y

L

L

G

G

P

P

V

Q

G

G

T

T

Y

F

S

T

E

H

Q

Q

A

A

-

A

-

V

-

N

-

A

-

Q

-

E

M

L

H

A

E

R

Y

F

F

E

G

P

Q

Q

S

G

I

F

E

D

G

L

L

M

P

P

C

M

P

D

S

D

I

V

D

P

E

Q

V

I

F

L

R

D

S

A

V

A

E

Q

A

H

G

G

A

D

A

G

E

-

F

W

G

G

V

I

V

V

P

A

V

W

E

E

N

N

S

N

T

V

E

E

G

G

A

Y

V

V

S

V

R

P

T

N

L

L

D

D

L

A

L

L

L

I

Q

D

T

A

Q

K

L

D

A

L

I

V

G

G

-

F

G

A

E

R

L

V

A

G

L

V

P

N

I

V

H

E

H

F

N

D

L

A

L

Y

T

V

L

A

N

Q

G

G

G

A

L

D

A

P

G

A

V

E

T

A

R

R

V

I

-

A

C

T

A

A

H

H

A

P

Q

H

A

G

L

L

A

A

Q

Q

C

C

Q

K

R

R

W

F

L

I

A

A

T

E

N

H

A

-

P

-

H

R

L

L

E

S

R

T

Q

Q

A

P

V

A

S

T

S

S

N

N

A

A

E

A

A

A

A

C

R

R

-

D

-

L

M

I

A

P

A

G

E

E

D

I

P

A

T

F

V

G

A

P

A

A

I

I

A

C

G

G

D

E

R

L

A

-

T

-

H

-

Y

Y

G

D

L

I

Q

T

V

R

A

I

Y

G

A

T

L

A

I

I

Q

Q

D

D

D

Y

P

Q

H

G

N

A

R

A

T
|
T

R

D

F

F

V

L

-

V

-

L

-

S

-

P

-

R

-

A

-

E

-

V

-

A

-

R

M

L

I

L

G

A

K

K

E

P

R

R

T

A

G

E

V

A

S

N

G

V

H

E

D

Y

Q

E

T

S

S

V

L

L

-

T

I

L

V

I

S

P

V

L

A

V

N

T

E
x
G

P

P

G

G

A

V

V
x
L

F

A

K

N

L

L

E

D

P

V

L

F

A

R

R

D

H

A

S

G

V

L

S

N

M

M

T

T

R

S

F

F

E

I

S

S

R

R

P

P

A

I

R

K

V

G

G

R

T

T

W

G

E

T

Y
|
Y

Y

S

F
|
F

Y

I

I

V

D

T

V

L

E

D

G

A

H

A

R

P

D

W

D

E

P

E

A

R

V

F

A

R

A

D

A

A

L

L

E

V

E

E

L

I

G

A

Q

E

K

H

A

G

A

D

F

W

L

A

K

K

I

T

L

L

G

A

S

V

Y

Y

P

P

R

R

active site: T177 (= T261)
binding 3-phenylpyruvic acid: G215 (≠ E288), L219 (≠ V292), Y248 (= Y321), F250 (= F323)

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
38% identity, 28% coverage: 258:357/360 of query aligns to 84:186/194 of 7alzA

query
sites
7alzA

H

H

N

N

R

T

T

T

R

R

F

F

V

Y

M

I

I

A

G

S

K

R

E

R

R

P

T

A

G

T

V

L

S

P

G

P

H

P

D

G

-

P

-

G

-

F

Q

M

T

T

S

T

L

L

I

L

V

F

S

R

V

V

A

N

N

N

E
x
Q

P

P

G

G

A

A

V
x
L

F

Y

K

K

L

A

L

L

E

G

P

G

L

L

A

A

R

T

H

A

S

G

V

V

S
x
N

M
|
M

T
|
T

R
|
R

F

L

E

E

S

S

R

Y

P

M

A

L

R

E

V

G

G

S

T

F

W

S

E

A

Y
x
T

Y

Q

F
|
F

Y

L

I

M

D

D

V

V

E

E

G

G

H

H

R

P

D

E

D

A

P

P

A

P

V

L

A

A

A

R

A

A

L

L

E

D

E

E

L

L

G

S

Q

F

K

F

A

S

A

E

F

Q

L

Q

K

E

I

I

L

L

G

G

S

V

Y

Y

P

P

binding phenylalanine: Q117 (≠ E288), L121 (≠ V292), N134 (≠ S305), M135 (= M306), T136 (= T307), R137 (= R308), T150 (≠ Y321), F152 (= F323)

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
29% identity, 31% coverage: 6:116/360 of query aligns to 1:112/352 of 5j6fA

query
sites
5j6fA

N

N

N

E

R

R

L

L

K

D

P

E

L

L

R

R

E

A

R

R

I

V

D

D

A

E

L

I

D

N

A

L

Q

Q

L

L

I

L

A

K

L

L

L

I

N

N

Q

E

R
|
R

A

G

A

R

V

L

A

V

L

Q

E

E

V
x
I

G

G

E

K

V

I

K
|
K

K

E

H

A

F

Q

N

G

A

T

P

H

V

R

F
x
Y

R
x
D

P

P

E

V

R
|
R

E

E

Q

R

Q

K

V
x
M

I

L

A

D

R

L

L

I

Q

S

D

E

M

H

S

N

E

D

G

G

P

P

L

F

A

E

S

T

E

S

H

T

I

L

S

Q

A

H

I

I

W

F

R

K

E

E

I

I

M

F

A

K

A

A

S

A

R

L

A

E

L

L

-

Q

E

E

K

D

T

D

I

H

K

R

A

K

A

A

Y

L

L

L

G

V

P

S

V

R

G

K

T

K

Y

H

S

P

E

E

Q

N

A

T

M

I

H

V

E

E

Y

V

F

K

G

G

Q

E

S

R

I

I

binding prephenic acid: R25 (= R30), I32 (≠ V37), K36 (= K41), Y44 (≠ F49), D45 (≠ R50), R48 (= R53), M52 (≠ V57)

Sites not aligning to the query:

binding manganese (ii) ion: 124, 294, 320, 331

6al9B Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
33% identity, 24% coverage: 5:90/360 of query aligns to 1:84/91 of 6al9B

query
sites
6al9B

L

L

N

D

N

S

R

L

L

L

K

E

P

N

L

L

R

R

E

A

R

E

I

I

D

D

A

A

L

L

D

D

A

N

Q

E

L

L

I

S

A

D

L

L

N

D

Q

K

R

R

A

L

A

E

V

I

A

A

L

L

E

K

V
x
I

G

A

E

L

V

I

K

K

K

Q

H

E

F

-

N

-

A

S

P

P

V

I

F

Y

R

C

P

P

E

K

R

R

E

E

Q

Q

Q

E

V

I

I

L

A

K

R

R

L

L

Q

S

D

Q

M

R

S

D

E

F

G

K

P

H

L

L

A

N

S

G

E

E

H

I

I

L

S

T

A

G

I

F

W

Y

R

T

E

E

I

V

M

F

A

K

A

I

S

S

R

R

A

K

L

F

E
x
Q

K

E

binding pyruvic acid: I33 (≠ V37), Q83 (≠ E89)

6al9A Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
33% identity, 23% coverage: 9:90/360 of query aligns to 4:83/90 of 6al9A

query
sites
6al9A

L

L

K

E

P

N

L

L

R

R

E

A

R

E

I

I

D

D

A

A

L

L

D

D

A

N

Q

E

L

L

I

S

A

D

L

L

N

D

Q

K

R
|
R

A

L

A

E

V

I

A

A

L

L

E

K

V

I

G

A

E

L

V

I

K

K

K

Q

H

E

F

-

N

-

A

S

P

P

V

I

F

Y

R
x
C

P

P

E

K

R
|
R

E
|
E

Q

Q

Q

E

V

I

I

L

A

K

R

R

L

L

Q

S

D

Q

M

R

S

D

E

F

G

K

P

H

L

L

A

N

S

G

E

E

H

I

I

L

S

T

A

G

I

F

W

Y

R

T

E

E

I

V

M
x
F

A

K

A

I

S
|
S

R

R

A

K

L

F

E
x
Q

K

E

binding prephenic acid: R25 (= R30), C43 (≠ R50), R46 (= R53), E47 (= E54), F75 (≠ M82), S78 (= S85), Q82 (≠ E89)

P39912 Protein AroA(G); EC 2.5.1.54; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see paper)
31% identity, 24% coverage: 6:90/360 of query aligns to 3:87/358 of P39912

query
sites
P39912

N

N

N

T

R

E

L

L

K

E

P

L

L

L

R

R

E

Q

R

K

I

A

D

D

A

E

L

L

D

N

A

L

Q

Q

L

I

I

L

A

K

L

L

L

I

N

N

Q

E

R

R

A

G

A

N

V

V

A

V

L

K

E

E

V

I

G

G

E

K

V

A

K

K

K

E

H

A

F

Q

N

G

A

V

P

N

V

R

F

F

R

D

P

P

E

V

R

R

E

E

Q

R

Q

T

V

M

I

L

A

N

R

N

L

I

Q

I

D

E

M

N

S

N

E

D

G

G

P

P

L

F

A

E

S

N

E

S

H

T

I

I

S

Q

A

H

I

I

W

F

R

K

E

E

I

I

M

F

A

K

A

A

S

G

R

L

A

E

L

L

E

Q

K

E

Sites not aligning to the query:

2 modified: Phosphoserine

5gmuB Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
31% identity, 24% coverage: 6:90/360 of query aligns to 2:86/87 of 5gmuB

query
sites
5gmuB

N

N

N

T

R

E

L

L

K

E

P

L

L

L

R

R

E

Q

R

K

I

A

D

D

A

E

L

L

D

N

A

L

Q

Q

L

I

I

L

A

K

L

L

L

I

N

N

Q

E

R
|
R

A

G

A

N

V

V

A

V

L

K

E

E

V

I

G

G

E

K

V

A

K

K

K

E

H

A

F

Q

N

G

A

V

P

N

V
x
R

F
|
F

R
x
D

P

P

E

V

R

R

E
|
E

Q

R

Q

T

V

M

I

L

A

N

R

N

L

I

Q

I

D

E

M

N

S

N

E

D

G

G

P

P

L

F

A

E

S

N

E

S

H

T

I

I

S

Q

A

H

I

I

W

F

R

K

E

E

I

I

M
x
F

A
x
K

A

A

S

G

R
x
L

A

E

L

L

E
x
Q

K

E

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: R26 (= R30), R44 (≠ V48), F45 (= F49), D46 (≠ R50), E50 (= E54), F78 (≠ M82), K79 (≠ A83), L82 (≠ R86), Q85 (≠ E89)

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
26% identity, 33% coverage: 12:128/360 of query aligns to 2:109/345 of 3nvtA

query
sites
3nvtA

L

L

R

R

E

T

R

Q

I

V

D

D

A

Q

L

L

D

N

A

I

Q

D

L

L

I

L

A

E

L

L

L

I

N

S

Q

K

R

R

A

A

A

N

V

L

A

V

L

Q

E

E

V

I

G

G

E

K

V

I

K

K

K

G

H

R

F

F

N

D

A

-

P

-

V

-

F

-

R

-

P

P

E

L

R

R

E

E

Q

R

Q

E

V

M

I

L

A

N

R

T

L

I

Q

L

D

A

M

A

S

N

E

E

G

G

P

P

L

F

A

E

S

D

E

S

H

T

I

V

S

Q

A

K

I

L

W

F

R

K

E

E

I

I

M

F

A

K

A

A

S

G

R

L

A

E

L

L

E

Q

K

E

T

E

I

D

K

H

A

S

A

K

Y

A

L

L

G

-

P

-

V

-

G

-

T

L

Y

V

S

S

E

R

Q

K

A

N

M

K

H

K

E

E

Y

D

F

T

G

I

Q

V

S

T

I

V

E

K

G

G

L

L

P

P

C

I

P

G

S

N

I

G

D

E

E

P

V

V

F

F

Sites not aligning to the query:

binding manganese (ii) ion: 114, 284, 310, 321

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
26% identity, 33% coverage: 12:128/360 of query aligns to 1:108/343 of 3tfcA

query
sites
3tfcA

L

L

R

R

E

T

R

Q

I

V

D

D

A

Q

L

L

D

N

A

I

Q

D

L

L

I

L

A

E

L

L

L

I

N

S

Q

K

R

R

A

A

A

N

V

L

A

V

L

Q

E

E

V

I

G

G

E

K

V

I

K

K

K

L

H

R

F

F

N

D

A

-

P

-

V

-

F

-

R

-

P

P

E

L

R

R

E

E

Q

R

Q

E

V

M

I

L

A

N

R

T

L

I

Q

L

D

A

M

A

S

N

E

E

G

G

P

P

L

F

A

E

S

D

E

S

H

T

I

V

S

Q

A

K

I

L

W

F

R

K

E

E

I

I

M

F

A

K

A

A

S

G

R

L

A

E

L

L

E

Q

K

E

T

E

I

D

K

H

A

S

A

K

Y

A

L

L

G

-

P

-

V

-

G

-

T

L

Y

V

S

S

E

R

Q

K

A

N

M

K

H

K

E

E

Y

D

F

T

G

I

Q

V

S

T

I

V

E

K

G

G

L

L

P

P

C

I

P

G

S

N

I

G

D

E

E

P

V

V

F

F

Sites not aligning to the query:

binding manganese (ii) ion: 113, 283, 309, 320
binding phosphoenolpyruvate: 137, 193, 196, 218, 248, 283

5jk5A Phenylalanine hydroxylase from dictyostelium - bh2 complex
30% identity, 21% coverage: 278:351/360 of query aligns to 4:84/400 of 5jk5A

query
sites
5jk5A

Q

H

T

S

S

S

L

I

I

F

V

F

S

S

V

I

A

S

-

K

-

G

-

S

N

D

E

K

P

I

G

G

A

G

V

L

F

L

K

E

L

Y

L

L

E

E

P

I

L

I

A

K

R

K

H

H

S

N

V

I

S

N

M

I

T

T

R

R

F

I

E

E

S

S

R

R

P

P

A

S

R

K

V

T

G

E

T

K

W

K

E

D

Y

Y

Y

D

F

F

Y

F

I

L

D

D

V

L

-

E

-

Y

-

P

-

T

E

E

G

N

H

N

R

K

D

E

D

V

P

E

A

K

V

V

A

I

A

K

A

D

L

L

E

E

L

K

G

G

Q

V

K

K

A

A

A

T

F

T

L

L

K

Q

Sites not aligning to the query:

active site: 259, 264, 304, 323
binding fe (iii) ion: 259, 264, 304
binding 7,8-dihydrobiopterin: 221, 222, 223, 228, 229, 296, 299
binding piperazine-n,n'-bis(2-ethanesulfonic acid): 212, 271, 275, 374

5jk8A Phenylalanine hydroxylase from dictyostelium - bh2, norleucine complex
30% identity, 21% coverage: 278:351/360 of query aligns to 4:84/390 of 5jk8A

query
sites
5jk8A

Q

H

T

S

S

S

L

I

I

F

V

F

S

S

V

I

A

S

-

K

-

G

-

S

N

D

E

K

P

I

G

G

A

G

V

L

F

L

K

E

L

Y

L

L

E

E

P

I

L

I

A

K

R

K

H

H

S

N

V

I

S

N

M

I

T

T

R

R

F

I

E

E

S

S

R

R

P

P

A

S

R
x
K

V

T

G

E

T

K

W

K

E

D

Y

Y

Y

D

F

F

Y

F

I

L

D

D

V

L

-

E

-

Y

-

P

-

T

E

E

G

N

H

N

R

K

D

E

D

V

P

E

A

K

V

V

A

I

A

K

A

D

L

L

E

E

L

K

G

G

Q

V

K

K

A

A

A

T

F

T

L

L

K

Q

binding piperazine-n,n'-bis(2-ethanesulfonic acid): K44 (≠ R315)

Sites not aligning to the query:

active site: 250, 255, 295, 314
binding fe (iii) ion: 250, 255, 295
binding 7,8-dihydrobiopterin: 214, 216, 219, 287, 290
binding norleucine: 242, 243, 250, 314, 315
binding piperazine-n,n'-bis(2-ethanesulfonic acid): 203, 226, 266, 365, 382

1ecmB Atomic structure of the buried catalytic pocket of escherichia coli chorismate mutase
31% identity, 26% coverage: 9:101/360 of query aligns to 2:95/95 of 1ecmB

query
sites
1ecmB

L

L

K

L

P

A

L

L

R

R

E

E

R

K

I

I

D

S

A

A

L

L

D

D

A

E

Q

K

L

L

I

L

A

A

L

L

N

A

Q

E

R
|
R

A

R

A

E

V

L

A

A

L

V

E

E

V
|
V

G

G

E

K

V

A

K
|
K

K

L

H

L

F

S

N

H

A

R

P

P

V

V

F
x
R

R
x
D

P

I

E

D

R
|
R

E
|
E

Q

R

Q

D

V

L

I

L

A

E

R

R

L

L

Q

I

D

T

M

L

S

G

E

K

G

A

-

H

P

H

L

L

A

D

S

A

E

H

H

Y

I

I

S

T

A

R

I

L

W

F

R

Q

E

L

I

I

M
x
I

A

E

A

D

S
|
S

R

V

A

L

L

T

E
x
Q

K

Q

T

A

I

L

K

L

A

Q

Y

H

L

L

G

N

P

K

V

I

G

N

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R23 (= R30), V30 (= V37), K34 (= K41), R42 (≠ F49), D43 (≠ R50), R46 (= R53), E47 (= E54), I76 (≠ M82), S79 (= S85), Q83 (≠ E89)

Query Sequence

>H281DRAFT_03925 H281DRAFT_03925 chorismate mutase
MDDELNNRLKPLRERIDALDAQLIALLNQRAAVALEVGEVKKHFNAPVFRPEREQQVIAR
LQDMSEGPLASEHISAIWREIMAASRALEKTIKAAYLGPVGTYSEQAMHEYFGQSIEGLP
CPSIDEVFRSVEAGAAEFGVVPVENSTEGAVSRTLDLLLQTQLAIGGELALPIHHNLLTL
NGGLAGVTRVCAHAQALAQCQRWLATNAPHLERQAVSSNAEAARMAAEDPTVAAIAGDRA
ATHYGLQVAYALIQDDPHNRTRFVMIGKERTGVSGHDQTSLIVSVANEPGAVFKLLEPLA
RHSVSMTRFESRPARVGTWEYYFYIDVEGHRDDPAVAAALEELGQKAAFLKILGSYPRAR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory