SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_04094 FitnessBrowser__Burk376:H281DRAFT_04094 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8RDH4 Dipeptide transport ATP-binding protein DppD; EC 7.4.2.9 from Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis) (see paper)
36% identity, 40% coverage: 45:342/744 of query aligns to 27:320/326 of Q8RDH4

query
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Q8RDH4

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ASGKST 44:49 (≠ GCGKST 62:67) binding
N61 (= N79) binding
Q97 (= Q115) binding
C285 (= C304) binding
C291 (= C310) binding
C298 (= C317) binding
C316 (= C338) binding

4fwiB Crystal structure of the nucleotide-binding domain of a dipeptide abc transporter (see paper)
35% identity, 40% coverage: 45:342/744 of query aligns to 26:309/310 of 4fwiB

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binding adenosine-5'-triphosphate: S42 (= S61), A43 (≠ G62), S44 (≠ C63), G45 (= G64), K46 (= K65), S47 (= S66), T48 (= T67), N60 (= N79), Q96 (= Q115), E179 (= E197), L267 (≠ P297)
binding magnesium ion: S47 (= S66), Q96 (= Q115), D178 (= D196)
binding iron/sulfur cluster: H247 (= H265), P248 (= P266), C274 (= C304), H277 (≠ A307), C280 (= C310), Y282 (≠ L312), A283 (= A313), C287 (= C317), P292 (= P322), C305 (= C338), H306 (= H339)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13, 20

P0AAH4 Putrescine export system ATP-binding protein SapD from Escherichia coli (strain K12) (see paper)
33% identity, 40% coverage: 23:317/744 of query aligns to 4:305/330 of P0AAH4

query
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P0AAH4

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K46 (= K65) mutation K->G,I,Q,R: 25% to 50% stimulation of K(+) uptake by TrkH.; mutation Missing: Almost no stimulation of K(+) uptake by TrkH.
G162 (= G175) mutation to A: 80% stimulation of K(+) uptake by TrkH.
Q165 (= Q178) mutation to L: 60% stimulation of K(+) uptake by TrkH.
D183 (= D196) mutation to E: About 60% stimulation of K(+) uptake by TrkH.; mutation D->K,N: 15% to 20% stimulation of K(+) uptake by TrkH.

7z15I E. Coli c-p lyase bound to a phnk/phnl dual abc dimer and adp + pi (see paper)
38% identity, 31% coverage: 389:619/744 of query aligns to 17:247/253 of 7z15I

query
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7z15I

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binding adenosine-5'-diphosphate: K17 (≠ R389), G18 (≠ A390), E37 (= E409), S38 (= S410), G39 (= G411), S40 (= S412), G41 (= G413), K42 (= K414), T43 (= T415), T44 (= T416), R136 (≠ Y508), T143 (≠ Q515), S145 (= S517), M148 (≠ L520)
binding magnesium ion: T43 (= T415), Q88 (= Q460), D168 (= D540)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13

7z18I E. Coli c-p lyase bound to a phnk abc dimer and atp (see paper)
38% identity, 31% coverage: 389:619/744 of query aligns to 17:247/250 of 7z18I

query
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A

L

K

K

L

S

M

I

L

S

G

A

L

R

L

L

A

T

P

P

D

Q

A

Q

G

G

S

E

V

I

L

H

E

Y

L

E

D

N

G

R

A

S

P

L

L

Y

A

A

A

M

R

S

V

E

T

A

R

D

R

R

N

R

D

R

E

L

Q

L

V

R

K

T

S

E

L

W

Q

G

I

V

V

V

F

H

Q
|
Q

N

H

P

P

D

L

S

D

A

G

L

L

N

R

R

R

A

Q

H

V

S

S

V

A

K

G

R

G

L

N

I

I

G

G

R

E

A

R

L

L

S

M

R

A

L

T

A

G

A

A

L

R

R

H

G

Y

A

G

A

D

I

I

D

R

E

A

R

T

L

A

A

Q

T

K

L

W

T

L

A

E

V

V

R

E

L

I

P

P

E

A

R

N

Y
x
R

L

I

G

D

S

D

R

L

T

P

R

T

Q
x
T

L
x
F

S
|
S

G
|
G

L
x
M

K

Q

Q

Q

R

R

V

L

A

Q

I

I

A

A

R

R

A

N

F

L

A

V

G

T

E

H

P

P

R

K

V

L

V

V

V

F

C

M

D

D

E

E

P

P

T

T

S

G

A

G

L

L

D

D

V

V

S

S

V

V

Q

Q

A

A

A

R

I

L

L

L

N

D

L

L

A

R

D

G

L

L

Q

V

R

V

E

E

R

L

G

N

V

L

S

A

Y

V

V

V

F

I

I

V

S

T

H
|
H

D

D

L

L

H

G

V

V

V

A

R

R

Y

L

L

L

S

A

D

D

R

R

I

L

A

L

V

V

L

M

Y

K

L

Q

G

G

R

Q

L

V

E

E

I

S

G

G

P

L

A

T

A

D

A

R

V

V

F

L

D

D

G

D

P

P

Q

H

H

H

P

P

Y

Y

T

T

E

Q

A

L

L

L

L

V

S

S

V

V

binding adenosine-5'-triphosphate: K17 (≠ R389), G18 (≠ A390), S38 (= S410), G39 (= G411), S40 (= S412), G41 (= G413), K42 (= K414), T43 (= T415), T44 (= T416), Q88 (= Q460), R136 (≠ Y508), T143 (≠ Q515), F144 (≠ L516), S145 (= S517), G146 (= G518), G147 (= G519), M148 (≠ L520), H202 (= H574)
binding magnesium ion: T43 (= T415), Q88 (= Q460)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13

7z16I E. Coli c-p lyase bound to phnk/phnl dual abc dimer with amppnp and phnk e171q mutation (see paper)
38% identity, 31% coverage: 389:619/744 of query aligns to 17:247/250 of 7z16I

query
sites
7z16I

R
x
K

A
x
G

V

F

D

S

K

D

V

V

S

S

L

F

D

D

L

L

A

W

A

P

G

G

E

E

T

V

L

L

G

G

L

I

V

V

G

G

E

E

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
|
T

L

L

A

L

K

K

L

S

M

I

L

S

G

A

L

R

L

L

A

T

P

P

D

Q

A

Q

G

G

S

E

V

I

L

H

E

Y

L

E

D

N

G

R

A

S

P

L

L

Y

A

A

A

M

R

S

V

E

T

A

R

D

R

R

N

R

D

R

E

L

Q

L

V

R

K

T

S

E

L

W

Q

G

I

V

V

V

F

H

Q

Q

N

H

P

P

D

L

S

D

A

G

L

L

N

R

R

R

A

Q

H

V

S

S

V

A

K

G

R

G

L

N

I

I

G

G

R

E

A

R

L

L

S

M

R

A

L

T

A

G

A

A

L

R

R

H

G

Y

A

G

A

D

I

I

D

R

E

A

R

T

L

A

A

Q

T

K

L

W

T

L

A

E

V

V

R

E

L

I

P

P

E

A

R

N

Y
x
R

L

I

G

D

S

D

R

L

T

P

R

T

Q
x
T

L

F

S
|
S

G
|
G

L
x
M

K

Q

Q

Q

R

R

V

L

A

Q

I

I

A

A

R

R

A

N

F

L

A

V

G

T

E

H

P

P

R

K

V

L

V

V

V

F

C

M

D

D

E
x
Q

P

P

T

T

S

G

A

G

L

L

D

D

V

V

S

S

V

V

Q

Q

A

A

A

R

I

L

L

L

N

D

L

L

A

R

D

G

L

L

Q

V

R

V

E

E

R

L

G

N

V

L

S

A

Y

V

V

V

F

I

I

V

S

T

H
|
H

D

D

L

L

H

G

V

V

V

A

R

R

Y

L

L

L

S

A

D

D

R

R

I

L

A

L

V

V

L

M

Y

K

L

Q

G

G

R

Q

L

V

E

E

I

S

G

G

P

L

A

T

A

D

A

R

V

V

F

L

D

D

G

D

P

P

Q

H

H

H

P

P

Y

Y

T

T

E

Q

A

L

L

L

L

V

S

S

V

V

binding phosphoaminophosphonic acid-adenylate ester: K17 (≠ R389), G18 (≠ A390), S38 (= S410), G39 (= G411), S40 (= S412), G41 (= G413), K42 (= K414), T43 (= T415), T44 (= T416), R136 (≠ Y508), T143 (≠ Q515), S145 (= S517), G146 (= G518), G147 (= G519), M148 (≠ L520), Q169 (≠ E541), H202 (= H574)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
34% identity, 30% coverage: 44:267/744 of query aligns to 44:258/382 of 7ahhC

query
sites
7ahhC

D

D

V

T

S

N

F

F

R

E

V

I

R

N

R

E

G

G

E

E

A

I

Y

F

G

V

L

I

V

M

G

G

E

L

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

-

M

-

A

-

L

L

R

R

Y

L

L

L

P

N

R

R

N

L

G

I

K

E

V

P

K

T

A

S

G

G

K

K

I

I

V

F

I

I

A

D

G

N

A

Q

D

D

V

V

N

A

K

T

L

L

D

N

A

K

D

E

A

D

L

L

R

L

T

Q

M

V

R

R

A

R

N

K

T

T

I

M

S

S

M

M

V

V

Y

F

Q

Q

D

N

P

-

A

-

R

F

A

G

L

L

N

F

P

P

S

H

L

R

T

T

I

I

A

L

R

E

Q

N

V

T

S

E

E

Y

A

G

F

L

E

E

A

V

A

Q

G

N

V

V

A

P

P

K

D

E

E

E

A

R

L

R

Q

K

R

R

S

A

L

E

E

K

M

A

L

L

Q

D

R

N

V

A

R

N

I

L

A

L

S

D

P

-

E

-

R

-

V

F

M

K

D

D

S

Q

Y

Y

P

P

H

K

Q

Q

L

L

S

S

G

G

M

M

Q

Q

R

R

V

V

V

G

I

L

A

A

M

R

A

A

L

L

A

A

S

N

N

D

P

P

A

E

L

I

L

L

I

L

L

M

D
|
D

E
|
E

P

A

T

F

T

S

G

A

L

L

D

D

A

P

T

L

V

I

E

R

A

R

E

E

V

M

L

Q

D

D

L

E

V

L

A

L

Q

E

L

L

R

Q

A

A

E

K

L

F

G

Q

T

K

A

T

V

I

L

I

F

F

I

V

S

S

H

H

N

D

L

L

A

N

V

E

I

A

G

L

R

R

M

I

C

G

E

D

R

R

V

I

G

A

V

I

L

M

Y

K

A

D

G

G

K

K

L

I

V

M

E

Q

E

I

G

G

A

T

T

G

Q

E

D

E

V

I

F

L

T

T

R

N

P

P

R

A

H

N

P

D

Y

Y

binding phosphoaminophosphonic acid-adenylate ester: S61 (= S61), G62 (= G62), S63 (≠ C63), G64 (= G64), K65 (= K65), S66 (= S66), T67 (= T67), D187 (= D196), E188 (= E197)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
34% identity, 30% coverage: 44:267/744 of query aligns to 44:258/382 of 7aheC

query
sites
7aheC

D

D

V

T

S

N

F

F

R

E

V

I

R

N

R

E

G

G

E

E

A

I

Y

F

G

V

L

I

V

M

G

G

E

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

V

L

A

-

M

-

A

-

L

L

R

R

Y

L

L

L

P

N

R

R

N

L

G

I

K

E

V

P

K

T

A

S

G

G

K

K

I

I

V

F

I

I

A

D

G

N

A

Q

D

D

V

V

N

A

K

T

L

L

D

N

A

K

D

E

A

D

L

L

R

L

T

Q

M

V

R

R

A

R

N

K

T

T

I

M

S

S

M

M

V

V

Y

F

Q

Q

D

N

P

-

A

-

R

F

A

G

L

L

N

F

P

P

S

H

L

R

T

T

I

I

A

L

R

E

Q

N

V

T

S

E

E

Y

A

G

F

L

E

E

A

V

A

Q

G

N

V

V

A

P

P

K

D

E

E

E

A

R

L

R

Q

K

R

R

S

A

L

E

E

K

M

A

L

L

Q

D

R

N

V

A

R

N

I

L

A

L

S

D

P

-

E

-

R

-

V

F

M

K

D

D

S

Q

Y

Y

P

P

H

K

Q

Q

L

L

S

S

G

G

M

M

Q

Q

R

R

V

V

V

G

I

L

A

A

M

R

A

A

L

L

A

A

S

N

N

D

P

P

A

E

L

I

L

L

I

L

L

M

D

D

E

E

P

A

T

F

T

S

G

A

L

L

D

D

A

P

T

L

V

I

E

R

A

R

E

E

V

M

L

Q

D

D

L

E

V

L

A

L

Q

E

L

L

R

Q

A

A

E

K

L

F

G

Q

T

K

A

T

V

I

L

I

F

F

I

V

S

S

H

H

N

D

L

L

A

N

V

E

I

A

G

L

R

R

M

I

C

G

E

D

R

R

V

I

G

A

V

I

L

M

Y

K

A

D

G

G

K

K

L

I

V

M

E

Q

E

I

G

G

A

T

T

G

Q

E

D

E

V

I

F

L

T

T

R

N

P

P

R

A

H

N

P

D

Y

Y

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 295, 296, 297, 298, 357, 372

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 34% coverage: 371:623/744 of query aligns to 1:248/343 of P30750

query
sites
P30750

V

M

L

I

R

K

A

L

E

S

N

N

L

I

S

T

K

K

T

V

F

F

H

H

V

Q

S

G

G

T

T

R

A

T

L

I

R

Q

A

A

V

L

D

N

K

N

V

V

S

S

L

L

D

H

L

V

A

P

A

A

G

G

E

Q

T

I

L

Y

G

G

L

V

V

I

G

G

E

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

A

I

K

R

L

C

M

V

L

N

G

L

L

L

L

E

A

R

P

P

D

T

A

E

G

G

S

S

V

V

L

L

E

-

L

V

D

D

G

G

A

Q

P

E

L

L

A

T

R

L

V

S

T

E

R

S

R

E

N

L

D

T

E

K

Q

A

V

R

K

R

S

Q

L

I

Q

G

I

M

V

I

F

F

Q

Q

N

H

P

F

D

N

S

L

A

L

L

S

N

S

R

R

A

T

H

-

S

-

V

-

K

-

R

-

L

V

I

F

G

G

R

N

A

V

L

A

S

L

R

P

L

L

A

E

A

-

L

L

R

D

G

N

A

T

A

P

I

K

D

D

E

E

-

V

-

K

-

R

R

R

L

V

A

T

T

E

L

L

T

L

A

S

A

L

V

V

R

G

L

L

P

G

E

D

R

K

Y

H

L

-

G

D

S

S

R

Y

T

P

R

S

Q

N

L

L

S

S

G

G

L

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

F

L

A

A

G

S

E

N

P

P

R

K

V

V

V

L

C

C

D

D

E
|
E

P

A

T

T

S

S

A

A

L

L

D

D

V

P

S

A

V

T

Q

T

A

R

A

S

I

I

L

L

N

E

L

L

A

K

D

D

L

I

Q

N

R

R

E

R

R

L

G

G

V

L

S

T

Y

I

V

L

F

L

I

I

S

T

H

H

D

E

L

M

H

D

V

V

R

K

Y

R

L

I

S

C

D

D

R

C

I

V

A

A

V

V

L

I

Y

S

L

N

G

G

R

E

L

L

L

I

E

E

I

Q

G

D

P

T

A

V

A

S

A

E

V

V

F

F

D

S

G

H

P

P

Q

K

H

T

P

P

Y

L

T

A

E

Q

A

K

L

F

L

I

S

Q

S

S

V

T

P

L

T

H

L

L

D

D

40:46 (vs. 410:416, 71% identical) binding
E166 (= E541) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

7ahdC Opua (e190q) occluded (see paper)
34% identity, 30% coverage: 44:267/744 of query aligns to 44:258/260 of 7ahdC

query
sites
7ahdC

D

D

V

T

S

N

F

F

R

E

V

I

R

N

R

E

G

G

E

E

A

I

Y

F

G

V

L

I

V

M

G

G

E

L

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

-

M

-

A

-

L

L

R

R

Y

L

L

L

P

N

R

R

N

L

G

I

K

E

V

P

K

T

A

S

G

G

K

K

I

I

V

F

I

I

A

D

G

N

A

Q

D

D

V

V

N

A

K

T

L

L

D

N

A

K

D

E

A

D

L

L

R

L

T

Q

M

V

R

R

A

R

N

K

T

T

I

M

S

S

M

M

V

V

Y

F

Q
|
Q

D

N

P

F

A

G

R

-

A

-

L

L

N

F

P

P

S

H

L

R

T

T

I

I

A

L

R

E

Q

N

V

T

S

E

E

Y

A

G

F

L

E

E

A

V

A

Q

G

N

V

V

A

P

P

K

D

E

E

E

A

R

L

R

Q

K

R

R

S

A

L

E

E

K

M

A

L

L

Q

D

R

N

V

A

R

N

I

L

A

L

S

D

P

-

E

-

R

-

V

F

M

K

D

D

S

Q

Y

Y

P

P

H
x
K

Q
|
Q

L

L

S
|
S

G

G

G
|
G

M
|
M

Q

Q

R

R

V

V

V

G

I

L

A

A

M

R

A

A

L

L

A

A

S

N

N

D

P

P

A

E

L

I

L

L

I

L

L

M

D

D

E
x
Q

P

A

T

F

T

S

G

A

L

L

D

D

A

P

T

L

V

I

E

R

A

R

E

E

V

M

L

Q

D

D

L

E

V

L

A

L

Q

E

L

L

R

Q

A

A

E

K

L

F

G

Q

T

K

A

T

V

I

L

I

F

F

I

V

S

S

H
|
H

N

D

L

L

A

N

V

E

I

A

G

L

R

R

M

I

C

G

E

D

R

R

V

I

G

A

V

I

L

M

Y

K

A

D

G

G

K

K

L

I

V

M

E

Q

E

I

G

G

A

T

T

G

Q

E

D

E

V

I

F

L

T

T

R

N

P

P

R

A

H

N

P

D

Y

Y

binding adenosine-5'-triphosphate: S61 (= S61), G62 (= G62), S63 (≠ C63), G64 (= G64), K65 (= K65), S66 (= S66), T67 (= T67), Q111 (= Q115), K161 (≠ H170), Q162 (= Q171), S164 (= S173), G166 (= G175), M167 (= M176), Q188 (≠ E197), H221 (= H230)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 29, 39

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
34% identity, 34% coverage: 371:623/744 of query aligns to 2:249/344 of 6cvlD

query
sites
6cvlD

V

M

L

I

R

K

A

L

E

S

N

N

L

I

S

T

K

K

T

V

F
|
F

H

H

V
x
Q

S

G

G

T

T

R

A

T

L
x
I

R

Q

A

A

V

L

D

N

K

N

V

V

S

S

L

L

D

H

L

V

A

P

A

A

G

G

E

Q

T

I

L

Y

G

G

L

V

V

I

G

G

E

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

A

I

K

R

L

C

M

V

L

N

G

L

L

L

L

E

A

R

P

P

D

T

A

E

G

G

S

S

V

V

L

L

E

-

L

V

D

D

G

G

A

Q

P

E

L

L

A

T

R

L

V

S

T

E

R

S

R

E

N

L

D

T

E

K

Q

A

V

R

K

R

S

Q

L

I

Q

G

I

M

V

I

F

F

Q

Q

N

H

P

F

D

N

S

L

A

L

L

S

N

S

R

R

A

T

H

-

S

-

V

-

K

-

R

-

L

V

I

F

G

G

R

N

A

V

L

A

S

L

R

P

L

L

A

E

A

-

L

L

R

D

G

N

A

T

A

P

I

K

D

D

E

E

-

V

-

K

-

R

R

R

L

V

A

T

T

E

L

L

T

L

A

S

A

L

V

V

R

G

L

L

P

G

E

D

R

K

Y

H

L

-

G

D

S

S

R

Y

T

P

R

S

Q
x
N

L
|
L

S
|
S

G
|
G

L
x
Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

F

L

A

A

G

S

E

N

P

P

R

K

V

V

V

L

C

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

V

P

S

A

V

T

Q

T

A

R

A

S

I

I

L

L

N

E

L

L

A

K

D

D

L

I

Q

N

R

R

E

R

R

L

G

G

V

L

S

T

Y

I

V

L

F

L

I

I

S

T

H
|
H

D

E

L

M

H

D

V

V

R

K

Y

R

L

I

S

C

D

D

R

C

I

V

A

A

V

V

L

I

Y

S

L

N

G

G

R

E

L

L

L

I

E

E

I

Q

G

D

P

T

A

V

A

S

A

E

V

V

F

F

D

S

G

H

P

P

Q

K

H

T

P

P

Y

L

T

A

E

Q

A

K

L

F

L

I

S

Q

S

S

V

T

P

L

T

H

L

L

D

D

binding phosphothiophosphoric acid-adenylate ester: F12 (= F381), Q14 (≠ V383), I19 (≠ L388), S41 (= S410), G42 (= G411), A43 (≠ S412), G44 (= G413), K45 (= K414), S46 (≠ T415), T47 (= T416), N141 (≠ Q515), L142 (= L516), S143 (= S517), G144 (= G518), G145 (= G519), Q146 (≠ L520), H200 (= H574)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
34% identity, 34% coverage: 371:623/744 of query aligns to 2:249/344 of 3tuzC

query
sites
3tuzC

V

M

L

I

R

K

A

L

E

S

N

N

L

I

S

T

K

K

T

V

F
|
F

H

H

V
x
Q

S

G

G

T

T

R

A

T

L

I

R

Q

A
|
A

V

L

D

N

K

N

V

V

S

S

L

L

D

H

L

V

A

P

A

A

G

G

E

Q

T

I

L

Y

G

G

L

V

V

I

G

G

E

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

A

I

K

R

L

C

M

V

L

N

G

L

L

L

L

E

A

R

P

P

D

T

A

E

G

G

S

S

V

V

L

L

E

-

L

V

D

D

G

G

A

Q

P

E

L

L

A

T

R

L

V

S

T

E

R

S

R

E

N

L

D

T

E

K

Q

A

V

R

K

R

S

Q

L

I

Q

G

I

M

V

I

F

F

Q

Q

N

H

P

F

D

N

S

L

A

L

L

S

N

S

R

R

A

T

H

-

S

-

V

-

K

-

R

-

L

V

I

F

G

G

R

N

A

V

L

A

S

L

R

P

L

L

A

E

A

-

L

L

R

D

G

N

A

T

A

P

I

K

D

D

E

E

-

V

-

K

-

R

R

R

L

V

A

T

T

E

L

L

T

L

A

S

A

L

V

V

R

G

L

L

P

G

E

D

R

K

Y

H

L

-

G

D

S

S

R

Y

T

P

R

S

Q

N

L

L

S

S

G

G

L

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

F

L

A

A

G

S

E

N

P

P

R

K

V

V

V

L

C

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

V

P

S

A

V

T

Q

T

A

R

A

S

I

I

L

L

N

E

L

L

A

K

D

D

L

I

Q

N

R

R

E

R

R

L

G

G

V

L

S

T

Y

I

V

L

F

L

I

I

S

T

H

H

D

E

L

M

H

D

V

V

R

K

Y

R

L

I

S

C

D

D

R

C

I

V

A

A

V

V

L

I

Y

S

L

N

G

G

R

E

L

L

L

I

E

E

I

Q

G

D

P

T

A

V

A

S

A

E

V

V

F

F

D

S

G

H

P

P

Q

K

H

T

P

P

Y

L

T

A

E

Q

A

K

L

F

L

I

S

Q

S

S

V

T

P

L

T

H

L

L

D

D

binding adenosine-5'-diphosphate: F12 (= F381), Q14 (≠ V383), A21 (= A390), G42 (= G411), A43 (≠ S412), G44 (= G413), K45 (= K414), S46 (≠ T415), T47 (= T416)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 312, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
34% identity, 34% coverage: 371:623/744 of query aligns to 2:249/344 of 3tuiC

query
sites
3tuiC

V

M

L

I

R

K

A

L

E

S

N

N

L

I

S

T

K

K

T

V

F
|
F

H

H

V
x
Q

S

G

G

T

T

R

A

T

L

I

R

Q

A

A

V

L

D

N

K

N

V

V

S

S

L

L

D

H

L

V

A

P

A

A

G

G

E

Q

T

I

L

Y

G

G

L

V

V

I

G

G

E

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

A

I

K

R

L

C

M

V

L

N

G

L

L

L

L

E

A

R

P

P

D

T

A

E

G

G

S

S

V

V

L

L

E

-

L

V

D

D

G

G

A

Q

P

E

L

L

A

T

R

L

V

S

T

E

R

S

R

E

N

L

D

T

E

K

Q

A

V

R

K

R

S

Q

L

I

Q

G

I

M

V

I

F

F

Q

Q

N

H

P

F

D

N

S

L

A

L

L

S

N

S

R

R

A

T

H

-

S

-

V

-

K

-

R

-

L

V

I

F

G

G

R

N

A

V

L

A

S

L

R

P

L

L

A

E

A

-

L

L

R

D

G

N

A

T

A

P

I

K

D

D

E

E

-

V

-

K

-

R

R

R

L

V

A

T

T

E

L

L

T

L

A

S

A

L

V

V

R

G

L

L

P

G

E

D

R

K

Y

H

L

-

G

D

S

S

R

Y

T

P

R

S

Q

N

L

L

S

S

G

G

L

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

F

L

A

A

G

S

E

N

P

P

R

K

V

V

V

L

C

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

V

P

S

A

V

T

Q

T

A

R

A

S

I

I

L

L

N

E

L

L

A

K

D

D

L

I

Q

N

R

R

E

R

R

L

G

G

V

L

S

T

Y

I

V

L

F

L

I

I

S

T

H

H

D

E

L

M

H

D

V

V

R

K

Y

R

L

I

S

C

D

D

R

C

I

V

A

A

V

V

L

I

Y

S

L

N

G

G

R

E

L

L

L

I

E

E

I

Q

G

D

P

T

A

V

A

S

A

E

V

V

F

F

D

S

G

H

P

P

Q

K

H

T

P

P

Y

L

T

A

E

Q

A

K

L

F

L

I

S

Q

S

S

V

T

P

L

T

H

L

L

D

D

binding adenosine-5'-diphosphate: F12 (= F381), Q14 (≠ V383), S41 (= S410), G42 (= G411), A43 (≠ S412), G44 (= G413), K45 (= K414), S46 (≠ T415), T47 (= T416)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
36% identity, 31% coverage: 41:273/744 of query aligns to 17:237/241 of 4u00A

query
sites
4u00A

V
|
V

L

L

Q

K

D

G

V

I

S

H

F

L

R

E

V

V

R

A

R

P

G

G

E

E

A

K

Y

L

G

V

L

I

V

I

G

G

E

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

-

M

-

A

-

L

I

R

R

Y

T

L

I

P

N

R

R

N

L

G

E

K

D

V

F

K

Q

A

E

G

G

K

E

I

V

V

V

I

V

A

D

G

G

A

L

D

S

V

V

N

K

K

-

L

-

D

D

A

D

D

R

A

A

L

L

R

R

T

E

M

I

R

R

A

-

N

R

T

E

I

V

S

G

M

M

V

V

Y

F

Q

Q

D

Q

P

-

A

-

R

F

A

N

L

L

N

F

P

P

S

H

L

M

T

T

I

V

A

L

R

E

Q

N

V

V

S

T

E

L

A

A

F

P

E

M

A

R

A

V

G

R

V

R

A

W

P

P

D

R

E

E

-

K

A

A

L

E

Q

K

R

K

S

A

L

L

E

E

M

L

L

L

Q

E

R

R

V

V

R

G

I

I

A

L

S

D

P

Q

E

A

R

R

V

-

M

-

D

-

S

K

Y

Y

P

P

H

A

Q

Q

L

L

S

S

G

G

M

Q

Q

Q

R

R

V

V

V

A

I

I

A

A

M

R

A

A

L

L

A

A

S

M

N

E

P

P

A

K

L

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

T

S

G

A

L

L

D

D

A

P

T

E

V

M

E

V

A

G

E

E

V

V

L

L

D

D

L

V

V

M

A

R

Q

D

L

L

R

-

A

A

E

Q

L

G

G

G

T

M

A

T

V

M

L

V

F

V

I

V

S

T

H

H

N

E

L

M

A

G

V

F

I

A

G

R

R

E

M

V

C

A

E

D

R

R

V

V

G

V

V

F

L

M

Y

D

A

G

G

G

K

Q

L

I

V

V

E

E

G

G

A

R

T

P

Q

E

D

E

V

I

F

F

T

T

R

R

P

P

R

K

H

E

P

E

Y

R

T

T

V

R

G

S

L

F

L

L

R

Q

binding adenosine-5'-diphosphate: V17 (= V41), S37 (= S61), G38 (= G62), S39 (≠ C63), G40 (= G64), K41 (= K65), S42 (= S66), T43 (= T67)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 15

3c4jA Abc protein artp in complex with atp-gamma-s
33% identity, 31% coverage: 40:272/744 of query aligns to 17:238/242 of 3c4jA

query
sites
3c4jA

E

E

V
|
V

L

L

Q

K

D

G

V

I

S

N

F

V

R

H

V

I

R

R

R

E

G

G

E

E

A

V

Y

V

G

V

L

V

V

I

G

G

E
x
P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

F

A

-

M

-

A

-

L

L

R

R

Y

C

L

L

P

N

R

L

N

L

G

E

K

D

V

F

K

D

A

E

G

G

K

E

I

I

V

I

I

I

A

D

G

G

A

I

D

N

V

L

N

K

K

A

L

K

D

D

A

T

D

N

A

-

L

L

R

N

T

K

M

V

R

R

A

E

N

E

T

-

I

V

S

G

M

M

V

V

Y

F

Q

Q

D

-

P

-

A

R

R

F

A

N

L

L

N

F

P

P

S

H

L

M

T

T

I

V

A

L

R

N

Q

N

V

I

S

T

E

L

A

A

F

P

E

M

A

K

A

V

G

R

V

K

A

W

P

P

D

R

E

E

-

K

A

A

L

E

Q

A

R

K

S

A

L

M

E

E

M

L

L

L

Q

D

R

K

V

V

R

G

I

L

A

K

S

D

P

K

E

A

R
x
H

V

-

M

-

D

-

S

A

Y
|
Y

P

P

H

D

Q

S

L

L

S

S

G

G

M

Q

Q

A

Q

Q

R

R

V

V

V

A

I

I

A

A

M

R

A

A

L

L

A

A

S

M

N

E

P

P

A

K

L

I

L

M

I

L

L

F

D

D

E
|
E

P

P

T

T

T

S

G

A

L

L

D

D

A

P

T

E

V

M

E

V

A

G

E

E

V

V

L

L

D

S

L

V

V

M

A

K

Q

Q

L

L

R

A

A

N

E

E

L

-

G

G

T

M

A

T

V

M

L

V

F

V

I

V

S

T

H

H

N

E

L

M

A

G

V

F

I

A

G

R

R

E

M

V

C

G

E

D

R

R

V

V

G

L

V

F

L

M

Y

D

A

G

G

G

K

Y

L

I

V

I

E

E

G

G

A

K

T

P

Q

E

D

D

V

L

F

F

T

D

R

R

P

P

R

Q

H

H

P

E

Y

R

T

T

V

K

G

A

L

F

L

L

binding phosphothiophosphoric acid-adenylate ester: V18 (= V41), P37 (≠ E60), S38 (= S61), G39 (= G62), S40 (≠ C63), G41 (= G64), K42 (= K65), S43 (= S66), T44 (= T67), H133 (≠ R163), Y135 (= Y168), E164 (= E197)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13

3c41J Abc protein artp in complex with amp-pnp/mg2+
33% identity, 31% coverage: 40:272/744 of query aligns to 17:238/242 of 3c41J

query
sites
3c41J

E

E

V
|
V

L

L

Q

K

D

G

V

I

S

N

F

V

R

H

V

I

R

R

R

E

G

G

E

E

A

V

Y

V

G

V

L

V

V

I

G

G

E
x
P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

F

A

-

M

-

A

-

L

L

R
|
R

Y

C

L

L

P

N

R

L

N

L

G

E

K

D

V

F

K

D

A

E

G

G

K

E

I

I

V

I

I

I

A

D

G

G

A

I

D

N

V

L

N

K

K

A

L

K

D

D

A

T

D

N

A

-

L

L

R

N

T

K

M

V

R

R

A

E

N

E

T

-

I

V

S

G

M

M

V

V

Y

F

Q

Q

D

-

P

-

A

R

R

F

A

N

L

L

N

F

P

P

S

H

L

M

T

T

I

V

A

L

R

N

Q

N

V

I

S

T

E

L

A

A

F

P

E

M

A

K

A

V

G

R

V

K

A

W

P

P

D

R

E

E

-

K

A

A

L

E

Q

A

R

K

S

A

L

M

E

E

M

L

L

L

Q

D

R

K

V

V

R

G

I

L

A

K

S

D

P

K

E

A

R
x
H

V

-

M

-

D

-

S

A

Y
|
Y

P

P

H

D

Q

S

L

L

S

S

G

G

M

Q

Q

A

Q

Q

R

R

V

V

V

A

I

I

A

A

M

R

A

A

L

L

A

A

S

M

N

E

P

P

A

K

L

I

L

M

I

L

L

F

D
|
D

E

E

P

P

T

T

T

S

G

A

L

L

D

D

A

P

T

E

V

M

E

V

A

G

E

E

V

V

L

L

D

S

L

V

V

M

A

K

Q

Q

L

L

R

A

A

N

E

E

L

-

G

G

T

M

A

T

V

M

L

V

F

V

I

V

S

T

H

H

N

E

L

M

A

G

V

F

I

A

G

R

R

E

M

V

C

G

E

D

R

R

V

V

G

L

V

F

L

M

Y

D

A

G

G

G

K

Y

L

I

V

I

E

E

G

G

A

K

T

P

Q

E

D

D

V

L

F

F

T

D

R

R

P

P

R

Q

H

H

P

E

Y

R

T

T

V

K

G

A

L

F

L

L

binding phosphoaminophosphonic acid-adenylate ester: V18 (= V41), P37 (≠ E60), S38 (= S61), G39 (= G62), S40 (≠ C63), G41 (= G64), K42 (= K65), S43 (= S66), T44 (= T67), R47 (= R74), H133 (≠ R163), Y135 (= Y168)
binding magnesium ion: S43 (= S66), D163 (= D196)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

2olkA Abc protein artp in complex with adp-beta-s
33% identity, 31% coverage: 40:272/744 of query aligns to 17:238/242 of 2olkA

query
sites
2olkA

E

E

V
|
V

L

L

Q

K

D

G

V

I

S

N

F

V

R

H

V

I

R

R

R

E

G

G

E

E

A

V

Y

V

G

V

L

V

V

I

G

G

E

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

F

A

-

M

-

A

-

L

L

R

R

Y

C

L

L

P

N

R

L

N

L

G

E

K

D

V

F

K

D

A

E

G

G

K

E

I

I

V

I

I

I

A

D

G

G

A

I

D

N

V

L

N

K

K

A

L

K

D

D

A

T

D

N

A

-

L

L

R

N

T

K

M

V

R

R

A

E

N

E

T

-

I

V

S

G

M

M

V

V

Y

F

Q

Q

D

-

P

-

A

R

R

F

A

N

L

L

N

F

P

P

S

H

L

M

T

T

I

V

A

L

R

N

Q

N

V

I

S

T

E

L

A

A

F

P

E

M

A

K

A

V

G

R

V

K

A

W

P

P

D

R

E

E

-

K

A

A

L

E

Q

A

R

K

S

A

L

M

E

E

M

L

L

L

Q

D

R

K

V

V

R

G

I

L

A

K

S

D

P

K

E

A

R

H

V

-

M

-

D

-

S

A

Y

Y

P

P

H

D

Q

S

L

L

S

S

G

G

M

Q

Q

A

Q

Q

R

R

V

V

V

A

I

I

A

A

M

R

A

A

L

L

A

A

S

M

N

E

P

P

A

K

L

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

T

S

G

A

L

L

D

D

A

P

T

E

V

M

E

V

A

G

E

E

V

V

L

L

D

S

L

V

V

M

A

K

Q

Q

L

L

R

A

A

N

E

E

L

-

G

G

T

M

A

T

V

M

L

V

F

V

I

V

S

T

H

H

N

E

L

M

A

G

V

F

I

A

G

R

R

E

M

V

C

G

E

D

R

R

V

V

G

L

V

F

L

M

Y

D

A

G

G

G

K

Y

L

I

V

I

E

E

G

G

A

K

T

P

Q

E

D

D

V

L

F

F

T

D

R

R

P

P

R

Q

H

H

P

E

Y

R

T

T

V

K

G

A

L

F

L

L

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: V18 (= V41), S38 (= S61), G39 (= G62), S40 (≠ C63), G41 (= G64), K42 (= K65), S43 (= S66), T44 (= T67)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 13, 16

2oljA Abc protein artp in complex with adp/mg2+
33% identity, 31% coverage: 40:272/744 of query aligns to 17:238/242 of 2oljA

query
sites
2oljA

E

E

V
|
V

L

L

Q

K

D

G

V

I

S

N

F

V

R

H

V

I

R

R

R

E

G

G

E

E

A

V

Y

V

G

V

L

V

V

I

G

G

E

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

F

A

-

M

-

A

-

L

L

R

R

Y

C

L

L

P

N

R

L

N

L

G

E

K

D

V

F

K

D

A

E

G

G

K

E

I

I

V

I

I

I

A

D

G

G

A

I

D

N

V

L

N

K

K

A

L

K

D

D

A

T

D

N

A

-

L

L

R

N

T

K

M

V

R

R

A

E

N

E

T

-

I

V

S

G

M

M

V

V

Y

F

Q

Q

D

-

P

-

A

R

R

F

A

N

L

L

N

F

P

P

S

H

L

M

T

T

I

V

A

L

R

N

Q

N

V

I