SitesBLAST
Comparing H281DRAFT_04220 H281DRAFT_04220 Cytochrome c, mono- and diheme variants to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
39% identity, 91% coverage: 36:428/432 of query aligns to 33:435/478 of Q47945
- Q37 (≠ T40) modified: Pyrrolidone carboxylic acid
Sites not aligning to the query:
8gy2B Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
39% identity, 89% coverage: 46:428/432 of query aligns to 4:397/433 of 8gy2B
- binding heme c: C18 (= C60), C21 (= C63), H22 (= H64), T46 (≠ P88), I48 (= I90), Y59 (≠ W101), L68 (≠ M110), R73 (≠ S115), V79 (≠ L121), Y80 (= Y122), M83 (≠ F125), F88 (≠ Y130), R126 (= R167), H165 (= H205), C166 (= C206), C169 (= C209), H170 (= H210), I201 (≠ Y236), A202 (= A237), P203 (= P238), L205 (= L240), W216 (= W251), F224 (≠ L259), A234 (= A269), V235 (= V270), F236 (= F271), F236 (= F271), M239 (= M274), N301 (= N333), C302 (= C334), C305 (= C337), H306 (= H338), M316 (≠ A348), F317 (= F349), P318 (= P350), L320 (= L352), P324 (≠ Q356), G342 (= G374), S352 (≠ H384), V354 (≠ Y386), M356 (= M388), F359 (= F391), M375 (≠ I407)
- binding ubiquinone-10: C21 (= C63), L34 (= L76), P39 (= P81), P81 (= P123), L129 (= L170), W132 (= W173), E168 (≠ M208), R173 (vs. gap), I197 (≠ L232), D241 (≠ E276)
Sites not aligning to the query:
7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
35% identity, 88% coverage: 48:428/432 of query aligns to 1:387/418 of 7w2jC
- binding heme c: C13 (= C60), C16 (= C63), H17 (= H64), T42 (≠ P88), I44 (= I90), Y55 (≠ W101), L75 (= L121), Y76 (= Y122), A78 (≠ G124), M79 (≠ F125), R122 (= R167), H161 (= H205), C162 (= C206), C165 (= C209), H166 (= H210), A191 (= A237), P192 (= P238), R223 (≠ A269), P227 (= P273), M228 (= M274), V289 (≠ N333), C290 (= C334), C293 (= C337), H294 (= H338), Y305 (vs. gap), Y306 (≠ F349), P307 (= P350), L309 (= L352), N312 (= N355), T313 (≠ Q356), T314 (≠ S357), D322 (≠ R368), I327 (≠ G373), V331 (≠ P377), R333 (≠ T379), I340 (≠ Y386), M342 (= M388), P343 (= P389), F345 (= F391)
8jejC Cryo-em structure of na-dithionite reduced membrane-bound fructose dehydrogenase from gluconobacter japonicus
35% identity, 88% coverage: 48:428/432 of query aligns to 1:401/413 of 8jejC
- binding heme c: C13 (= C60), C16 (= C63), H17 (= H64), T42 (≠ P88), I44 (= I90), F60 (= F106), L64 (≠ M110), L75 (= L121), Y76 (= Y122), M79 (≠ F125), P80 (= P126), Y84 (= Y130), R122 (= R167), C162 (= C206), C165 (= C209), H166 (= H210), I186 (= I230), W189 (= W235), A191 (= A237), P192 (= P238), I194 (≠ L240), W205 (= W251), Y213 (≠ L259), R223 (≠ A269), M228 (= M274), V303 (≠ N333), C304 (= C334), C307 (= C337), H308 (= H338), Y320 (≠ F349), P321 (= P350), L323 (= L352), T327 (≠ Q356), T328 (≠ S357), D336 (≠ R368), I341 (≠ G373), V345 (≠ P377), R347 (≠ T379), I354 (≠ Y386), M356 (= M388), F359 (= F391), I376 (≠ V403)
- binding ubiquinone-10: M36 (≠ F82), P77 (= P123), S124 (≠ M169), W128 (= W173), C165 (= C209), L173 (≠ G217)
Sites not aligning to the query:
8gy3A Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
34% identity, 85% coverage: 45:410/432 of query aligns to 38:410/440 of 8gy3A
- binding heme c: Y52 (≠ D59), C53 (= C60), C56 (= C63), H57 (= H64), S84 (≠ P88), I86 (= I90), W97 (= W101), F102 (= F106), L117 (= L121), F121 (= F125), F126 (≠ Y130), R163 (= R167), C203 (= C206), C206 (= C209), H207 (= H210), A232 (= A237), P233 (= P238), L235 (= L240), W245 (= W251), Y253 (≠ L259), L254 (= L260), G263 (= G268), S264 (≠ A269), M269 (= M274), Y292 (≠ L297), C337 (= C334), C340 (= C337), H341 (= H338), P353 (= P350), L355 (= L352), N358 (= N355), N359 (≠ Q356), V372 (≠ M369), I377 (≠ P377), G382 (≠ N382), Q383 (≠ P383), I386 (≠ Y386), M388 (= M388), F391 (= F391)
- binding ubiquinone-10: E55 (≠ A62), T76 (= T80), F78 (= F82), Y118 (= Y122), P119 (= P123), I160 (≠ F164), G166 (= G172), Q167 (vs. gap), F169 (vs. gap), W170 (= W173), H202 (= H205), R210 (≠ I213), L213 (≠ M216)
2zooA Crystal structure of nitrite reductase from pseudoalteromonas haloplanktis tac125
35% identity, 25% coverage: 321:427/432 of query aligns to 334:438/438 of 2zooA
- binding protoporphyrin ix containing fe: C347 (= C334), C350 (= C337), H351 (= H338), F362 (= F349), P363 (= P350), P364 (= P351), L365 (= L352), S368 (≠ N355), Y370 (≠ S357), I382 (≠ V370), L386 (≠ G374), S387 (≠ Y375), G388 (≠ P376), I390 (≠ S378), V392 (≠ D380), Y397 (≠ P385), N398 (≠ Y386), G399 (= G387), V400 (≠ M388), M401 (≠ P389)
Sites not aligning to the query:
- active site: 81, 84, 86, 121, 122, 130, 135, 227, 249, 250, 276
- binding copper (ii) ion: 81, 86, 121, 122, 130, 135
5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
35% identity, 25% coverage: 323:430/432 of query aligns to 350:456/456 of 5oboA
- binding heme c: T360 (≠ N333), C361 (= C334), C364 (= C337), H365 (= H338), P377 (= P350), P378 (= P351), L379 (= L352), S382 (≠ N355), F384 (≠ S357), I395 (≠ P366), N401 (= N372), G402 (= G373), S413 (≠ Y386), M415 (= M388), M418 (≠ F391)
Sites not aligning to the query:
- active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
- binding copper (ii) ion: 91, 96, 131, 132, 140, 145
- binding nitrite ion: 94, 96, 131
6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
35% identity, 25% coverage: 323:430/432 of query aligns to 349:455/455 of 6fjaA
- binding protoporphyrin ix containing fe: T359 (≠ N333), C360 (= C334), C363 (= C337), H364 (= H338), P376 (= P350), P377 (= P351), L378 (= L352), F383 (≠ S357), N400 (= N372), G401 (= G373), Y410 (≠ H384), S412 (≠ Y386), M414 (= M388), M417 (≠ F391)
Sites not aligning to the query:
- active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
- binding copper (ii) ion: 90, 131, 139, 144
4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
34% identity, 25% coverage: 323:428/432 of query aligns to 352:456/457 of 4ax3D
- binding heme c: C363 (= C334), C366 (= C337), H367 (= H338), P379 (= P350), P380 (= P351), L381 (= L352), S384 (≠ N355), F386 (≠ S357), N403 (= N372), G404 (= G373), S415 (≠ Y386), M417 (= M388), M420 (≠ F391)
Sites not aligning to the query:
- active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
- binding copper (ii) ion: 93, 98, 133, 134, 142, 147
8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase
31% identity, 23% coverage: 328:427/432 of query aligns to 24:121/121 of 8hddB
- binding protoporphyrin ix containing fe: C30 (= C334), C33 (= C337), H34 (= H338), Y46 (vs. gap), P47 (= P350), T54 (≠ S357), V66 (≠ M369), I67 (≠ V370), R73 (≠ T379), I80 (≠ Y386), M82 (= M388), P83 (= P389)
1dt1A Thermus thermophilus cytochrome c552 synthesized by escherichia coli (see paper)
38% identity, 20% coverage: 326:413/432 of query aligns to 2:91/129 of 1dt1A
- binding heme c: C9 (= C334), C12 (= C337), H13 (= H338), P25 (= P350), H30 (≠ N355), Y43 (≠ P366), V47 (= V370), Q53 (≠ P376), G54 (≠ P377), G65 (vs. gap), M67 (= M388), F70 (= F391)
Sites not aligning to the query:
1r0qA Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (spirographis) heme (see paper)
38% identity, 20% coverage: 326:413/432 of query aligns to 3:92/130 of 1r0qA
- binding 2-formyl-protoporphryn ix: C13 (= C337), H14 (= H338), P26 (= P350), L28 (= L352), H31 (≠ N355), Y44 (≠ P366), V48 (= V370), Q54 (≠ P376), G55 (≠ P377), G66 (vs. gap), M68 (= M388)
Sites not aligning to the query:
1qyzA Characterization of the malformed, recombinant cytochrome rc552 (see paper)
38% identity, 20% coverage: 326:413/432 of query aligns to 3:92/130 of 1qyzA
- binding 2-acetyl-protoporphyrin ix: Y7 (= Y330), C10 (= C334), C13 (= C337), H14 (= H338), P26 (= P350), H31 (≠ N355), Y44 (≠ P366), Q54 (≠ P376), G55 (≠ P377), G66 (vs. gap), M68 (= M388), F71 (= F391)
Sites not aligning to the query:
1c52A Thermus thermophilus cytochrome-c552: a new highly thermostable cytochromE-C structure obtained by mad phasing (see paper)
38% identity, 20% coverage: 326:413/432 of query aligns to 4:93/131 of 1c52A
- binding protoporphyrin ix containing fe: C11 (= C334), C14 (= C337), H15 (= H338), F26 (= F349), P27 (= P350), L29 (= L352), H32 (≠ N355), Y45 (≠ P366), L54 (≠ Y375), Q55 (≠ P376), G56 (≠ P377), G67 (vs. gap), M69 (= M388), F72 (= F391)
Sites not aligning to the query:
Query Sequence
>H281DRAFT_04220 H281DRAFT_04220 Cytochrome c, mono- and diheme variants
MKRKSLFALSAVVVVATAALVPVLWSGGDNLHNGSAVAATPADQAALVKKGEYLARAGDC
IACHTVRGGKQFAGGLPMATPFGTMFTPNITPDDQYGIGKWTQDDFYRAMHTGRSKDGSL
LYPGFPFTSYTKVTRADSDAIYAYLRSVTPVNVASRPHELKFPFNQRNMLIGWRTLFFRE
GEYKADPTKSVEWNRGAYLIEGLGHCGMCHTSINAMGGPVSSAAFAGGLIPLQNWYAPSL
TSNKEAGLGDWETKDIADLLKTGVSNRGAVFGPMAEVVHNSLQYMTDADINAMATYLKTI
PQKSEAPEPLQLETSEKFGGELLKQGQKIYADNCAKCHADNGLGQPPAFPPLANNQSIQM
PSAVNPIRMVLNGGYPPSTDANPHPYGMPPFAQSLSNQEVAAVVTYIRMSWGNHGTAVSP
QQVSDLRSAPLD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory