SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_04220 H281DRAFT_04220 Cytochrome c, mono- and diheme variants to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
39% identity, 91% coverage: 36:428/432 of query aligns to 33:435/478 of Q47945

query
sites
Q47945

A
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x
A

A
x
H

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x
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Q37 (≠ T40) modified: Pyrrolidone carboxylic acid

Sites not aligning to the query:

1:36 signal peptide

7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
35% identity, 88% coverage: 48:428/432 of query aligns to 1:387/418 of 7w2jC

query
sites
7w2jC

V

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N

M
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Y

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F

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Y

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A

binding heme c: C13 (= C60), C16 (= C63), H17 (= H64), I39 (≠ M85), S41 (≠ T87), T42 (≠ P88), N43 (= N89), I44 (= I90), I52 (= I98), Y55 (≠ W101), F60 (= F106), A63 (= A109), L75 (= L121), Y76 (= Y122), A78 (≠ G124), M79 (≠ F125), P80 (= P126), Y84 (= Y130), R122 (= R167), H161 (= H205), C162 (= C206), C165 (= C209), H166 (= H210), W189 (= W235), R190 (≠ Y236), A191 (= A237), P192 (= P238), I194 (≠ L240), W205 (= W251), Y213 (≠ L259), L214 (= L260), R223 (≠ A269), P227 (= P273), M228 (= M274), L236 (= L282), V289 (≠ N333), C290 (= C334), C293 (= C337), H294 (= H338), Y305 (vs. gap), Y306 (≠ F349), P307 (= P350), L309 (= L352), N312 (= N355), T313 (≠ Q356), T314 (≠ S357), T314 (≠ S357), Q317 (≠ A363), D322 (≠ R368), L323 (≠ M369), S326 (≠ N372), I327 (≠ G373), V331 (≠ P377), R333 (≠ T379), I340 (≠ Y386), M342 (= M388), P343 (= P389), F345 (= F391)

2zooA Crystal structure of nitrite reductase from pseudoalteromonas haloplanktis tac125
35% identity, 25% coverage: 321:427/432 of query aligns to 334:438/438 of 2zooA

query
sites
2zooA

E

E

L

Q

L

I

K

R

Q

F

G

G

Q

Q

K

R

I

V

Y

Y

A

E

D

A

N
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N

C
|
C

A

M

K

A

C
|
C

H
|
H

A

Q

D

A

N

N

G

G

L

E

G

G

Q

I

P
|
P

P
x
G

A
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A

F
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F

P
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P

L
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L

A

A

N

K

N
x
S

Q

D

S
x
Y

I
x
L

Q

N

M

N

P

-

S

-

A

-

V

-

N

N

P

P

-

L

-

G

I

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A

V
x
I

L

I

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G

G

G
x
L

Y
x
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G

P

P

S
x
I

T

K

D
x
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A

N

N

N

P

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H

N

P
x
Y

Y
x
N

G
|
G

M
x
V

P
x
M

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A

A

Q
x
M

S

N

L

L

S

N

N

D

Q

E

E

D

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A

A

A

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V
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V

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I

T

T

Y

F

I

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M

N

S

N

W

W

G

D

N

N

H

A

G

G

T

G

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binding beta-D-fructofuranose: P359 (= P346), G360 (≠ P347), A361 (= A348)
binding protoporphyrin ix containing fe: N346 (= N333), C347 (= C334), C350 (= C337), H351 (= H338), F362 (= F349), P363 (= P350), P364 (= P351), L365 (= L352), S368 (≠ N355), Y370 (≠ S357), L371 (≠ I358), I382 (≠ V370), L386 (≠ G374), S387 (≠ Y375), G388 (≠ P376), I390 (≠ S378), V392 (≠ D380), Y397 (≠ P385), N398 (≠ Y386), G399 (= G387), V400 (≠ M388), M401 (≠ P389), M404 (≠ Q393), V414 (= V403)

Sites not aligning to the query:

active site: 81, 84, 86, 121, 122, 130, 135, 227, 249, 250, 276
binding copper (ii) ion: 81, 86, 121, 122, 130, 135

5ocbA Crystal structure of nitric oxide bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
35% identity, 25% coverage: 323:430/432 of query aligns to 350:456/456 of 5ocbA

query
sites
5ocbA

L

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K

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Q

A

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G

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A

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L

Y

F

A

A

D

G

N
x
T

C
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C

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C
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C

H
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H

A

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D

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N

G

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P

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L

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L

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M

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F
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M

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L

L

S

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N

D

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D

E

E

V

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A

A

A

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x
I

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T

Y

Y

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W

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binding heme c: T360 (≠ N333), C361 (= C334), C364 (= C337), H365 (= H338), P377 (= P350), P378 (= P351), L379 (= L352), S382 (≠ N355), F384 (≠ S357), L385 (≠ I358), I395 (≠ P366), V396 (≠ I367), L400 (= L371), N401 (= N372), G402 (= G373), I404 (≠ Y375), V406 (≠ T379), Y411 (≠ H384), D412 (≠ P385), S413 (≠ Y386), V414 (≠ G387), M415 (= M388), M418 (≠ F391), I429 (≠ V403)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitric oxide: 94, 131

5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
35% identity, 25% coverage: 323:430/432 of query aligns to 350:456/456 of 5oboA

query
sites
5oboA

L

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Q

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G

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A

A

D

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N
x
T

C
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C

A

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C
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H

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N

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G

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A

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G

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L

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G

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L

A

A

N

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Q

A

M

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-

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I

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N
|
N

G
|
G

G

K

Y
x
I

P

-

S

K

T
x
V

D

N

A

G

N

Q

P

E

H
x
Y

P
x
D

Y
x
S

G
x
V

M
|
M

P

P

F
x
M

A

T

Q

Q

S

-

L

L

S

N

N

D

Q

D

E

E

V

V

A

A

A

N

V

I

V

L

T

T

Y

Y

I

V

R

L

M

N

S

S

W

W

G

D

N

N

H

P

G

G

T

G

A

R

V

V

S

S

P

A

Q

E

Q

D

V

V

S

K

D

K

L

V

R

R

S

A

A

Q

P

P

binding heme c: T360 (≠ N333), C361 (= C334), C364 (= C337), H365 (= H338), P377 (= P350), P378 (= P351), L379 (= L352), S382 (≠ N355), F384 (≠ S357), L385 (≠ I358), I395 (≠ P366), V396 (≠ I367), L400 (= L371), N401 (= N372), G402 (= G373), I404 (≠ Y375), V406 (≠ T379), Y411 (≠ H384), D412 (≠ P385), S413 (≠ Y386), V414 (≠ G387), M415 (= M388), M418 (≠ F391)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitrite ion: 94, 96, 131

6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
35% identity, 25% coverage: 323:430/432 of query aligns to 349:455/455 of 6fjaA

query
sites
6fjaA

L

V

K

Q

Q

A

G

G

Q

R

K

A

I

L

Y

F

A

A

D

G

N
x
T

C
|
C

A

S

K

V

C
|
C

H
|
H

A

Q

D

G

N

N

G

G

L

A

G

G

Q

L

P

P

P

G

A

V

F

F

P
|
P

L
|
L

A

A

N

K

N
x
S

Q

D

S
x
F

I
x
L

Q

A

M

A

-

D

-

P

P

K

S

R

A

A

V

M

N

N

P
x
I

I
x
V

R

L

M

H

V

G

L
|
L

N
|
N

G
|
G

G

K

Y
x
I

P

-

S

K

T
x
V

D

N

A

G

N

Q

P

E

H
x
Y

P
x
D

Y
x
S

G
x
V

M
|
M

P

P

F
x
M

A

T

Q

Q

S

-

L

L

S

N

N

D

Q

D

E

E

V

V

A

A

A

N

V
x
I

V

L

T

T

Y

Y

I

V

R

L

M

N

S

S

W

W

G

D

N

N

H

P

G

G

T

G

A

R

V

V

S

S

P

A

Q

E

Q

D

V

V

S

K

D

K

L

V

R

R

S

A

A

Q

P

P

binding protoporphyrin ix containing fe: T359 (≠ N333), C360 (= C334), C363 (= C337), H364 (= H338), P376 (= P350), P377 (= P351), L378 (= L352), S381 (≠ N355), F383 (≠ S357), L384 (≠ I358), I394 (≠ P366), V395 (≠ I367), L399 (= L371), N400 (= N372), G401 (= G373), I403 (≠ Y375), V405 (≠ T379), Y410 (≠ H384), D411 (≠ P385), S412 (≠ Y386), V413 (≠ G387), M414 (= M388), M417 (≠ F391), I428 (≠ V403)

Sites not aligning to the query:

active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
binding copper (ii) ion: 90, 131, 139, 144

4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
34% identity, 25% coverage: 323:428/432 of query aligns to 352:456/457 of 4ax3D

query
sites
4ax3D

L

V

K

Q

Q

A

G

G

Q

R

K

A

I

L

Y

F

A

A

D

G

N
x
T

C
|
C

A

S

K

V

C
|
C

H
|
H

A

Q

D

G

N

N

G

G

L

A

G

G

Q

L

P

P

P

G

A

V

F

F

P
|
P

L
|
L

A

A

N

K

N
x
S

Q

D

S
x
F

I
x
L

Q

A

M

A

-

D

-

P

P

K

S

R

A

A

V

M

N

N

P
x
I

I
x
V

R

L

M

H

V

G

L
|
L

N
|
N

G
|
G

G

K

Y
x
I

P

-

S

K

T
x
V

D

N

A

G

N

Q

P

E

H
x
Y

P
x
D

Y
x
S

G
x
V

M
|
M

P

P

F
x
M

A

T

Q

Q

S

-

L

L

S

N

N

D

Q

D

E

E

V

V

A

A

A

N

V
x
I

V

L

T

T

Y

Y

I

V

R

L

M

N

S

S

W

W

G

D

N

N

H

P

G

G

T

G

A

R

V

V

S

S

P

A

Q

E

Q

D

V

V

S

K

D

K

L

V

R

R

S

A

binding heme c: T362 (≠ N333), C363 (= C334), C366 (= C337), H367 (= H338), P379 (= P350), P380 (= P351), L381 (= L352), S384 (≠ N355), F386 (≠ S357), L387 (≠ I358), I397 (≠ P366), V398 (≠ I367), L402 (= L371), N403 (= N372), G404 (= G373), I406 (≠ Y375), V408 (≠ T379), Y413 (≠ H384), D414 (≠ P385), S415 (≠ Y386), V416 (≠ G387), M417 (= M388), M420 (≠ F391), I431 (≠ V403)

Sites not aligning to the query:

active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
binding copper (ii) ion: 93, 98, 133, 134, 142, 147

8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase
31% identity, 23% coverage: 328:427/432 of query aligns to 24:121/121 of 8hddB

query
sites
8hddB

K

R

I

L

Y

Y

A

L

D

G

N
|
N

C
|
C

A

A

K

T

C
|
C

H
|
H

A

Q

D

M

N

Q

G

G

L

K

G

G

Q

T

P

P

P

D

A

G

F
x
Y

-
x
Y

P
|
P

P

S

L

L

A

F

N

H

N

N

Q

S

S
x
T

I

V

Q

G

M

A

P

S

S

N

A

P

V

S

N

N

P

L

I

V

R

Q

M
x
V

V
x
I

L

L

N

N

G

G

G

-

Y

-

P

-

P

V

S

Q

T
x
R

D

K

A

I

N

G

P

S

H

E

P

D

Y
x
I

G

G

M
|
M

P
|
P

P

A

F
|
F

A

R

Q

Y

S

D

L

L

S

N

N

D

Q

A

E

Q

V

I

A

A

V

L

V

T

T

N

Y

Y

I

V

R

T

M

A

S

Q

W

F

G

G

N

N

H

P

G

A

T

A

A

K

V

V

S

T

P

E

Q

Q

Q

D

V

V

S

A

D

K

L

L

R

R

binding protoporphyrin ix containing fe: N29 (= N333), C30 (= C334), C33 (= C337), H34 (= H338), Y45 (≠ F349), Y46 (vs. gap), P47 (= P350), T54 (≠ S357), V66 (≠ M369), I67 (≠ V370), R73 (≠ T379), I80 (≠ Y386), M82 (= M388), P83 (= P389), F85 (= F391)

1dt1A Thermus thermophilus cytochrome c552 synthesized by escherichia coli (see paper)
38% identity, 20% coverage: 326:413/432 of query aligns to 2:91/129 of 1dt1A

query
sites
1dt1A

G

G

Q

A

K

K

I

I

Y

Y

A

A

D

-

N
x
Q

C
|
C

A

A

K

G

C
|
C

H
|
H

A

Q

D

Q

N

N

G

G

L

Q

G

G

Q

I

P

P

P

G

A

A

F
|
F

P
|
P

P

P

L
|
L

A

A

N

G

N
x
H

-

V

-

A

Q

E

S
x
I

I

L

Q

A

M

K

P

E

S

G

A

G

V

R

N

E

P
x
Y

I

L

R

I

M

L

V
|
V

L
|
L

N

L

G

Y

G

G

Y
x
L

P
x
Q

P
x
G

S

Q

T
x
I

D

E

A

V

N

K

P

G

H

M

P

K

Y
|
Y

G
x
N

-
x
G

-
x
V

M
|
M

P

S

F
|
F

A

A

Q

Q

S

-

L

L

S

K

N

D

Q

E

E

E

V

I

A

A

V

V

V

L

T

N

Y

H

I
|
I

R

A

M

T

S

A

W

W

G

G

N

D

binding heme c: Q8 (≠ N333), C9 (= C334), C12 (= C337), H13 (= H338), F24 (= F349), P25 (= P350), L27 (= L352), H30 (≠ N355), I34 (≠ S357), Y43 (≠ P366), V47 (= V370), L48 (= L371), L52 (≠ Y375), Q53 (≠ P376), G54 (≠ P377), I56 (≠ T379), Y63 (= Y386), N64 (≠ G387), G65 (vs. gap), V66 (vs. gap), M67 (= M388), F70 (= F391), I85 (= I407)

Sites not aligning to the query:

binding heme c: 123

1r0qA Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (spirographis) heme (see paper)
38% identity, 20% coverage: 326:413/432 of query aligns to 3:92/130 of 1r0qA

query
sites
1r0qA

G

G

Q

A

K

K

I

I

Y
|
Y

A

A

D

-

N

Q

C
|
C

A

A

K

G

C
|
C

H
|
H

A

Q

D

Q

N

N

G

G

L

Q

G

G

Q

I

P

P

P

G

A

A

F
|
F

P
|
P

P

P

L
|
L

A

A

N

G

N
x
H

-

V

-

A

Q

E

S
x
I

I

L

Q

A

M

K

P

E

S

G

A

G

V

R

N

E

P
x
Y

I

L

R

I

M

L

V
|
V

L
|
L

N

L

G

Y

G

G

Y
x
L

P
x
Q

P
x
G

S

Q

T
x
I

D

E

A

V

N

K

P

G

H

M

P

K

Y
|
Y

G
x
N

-
x
G

-
x
V

M
|
M

P

S

F
|
F

A

A

Q

Q

S

-

L

L

S

K

N

D

Q

E

E

E

V

I

A

A

V
|
V

V

L

T

N

Y

H

I
|
I

R

A

M

T

S

A

W

W

G

G

N

D

binding 2-formyl-protoporphryn ix: Y7 (= Y330), C10 (= C334), C13 (= C337), H14 (= H338), F25 (= F349), P26 (= P350), L28 (= L352), H31 (≠ N355), I35 (≠ S357), Y44 (≠ P366), V48 (= V370), L49 (= L371), L53 (≠ Y375), Q54 (≠ P376), G55 (≠ P377), I57 (≠ T379), Y64 (= Y386), N65 (≠ G387), G66 (vs. gap), V67 (vs. gap), M68 (= M388), F71 (= F391), V82 (= V403), I86 (= I407)

Sites not aligning to the query:

binding 2-formyl-protoporphryn ix: 124

1qyzA Characterization of the malformed, recombinant cytochrome rc552 (see paper)
38% identity, 20% coverage: 326:413/432 of query aligns to 3:92/130 of 1qyzA

query
sites
1qyzA

G

G

Q

A

K

K

I

I

Y
|
Y

A

A

D

-

N

Q

C
|
C

A

A

K

G

C
|
C

H
|
H

A

Q

D

Q

N

N

G

G

L

Q

G

G

Q

I

P

P

P

G

A

A

F
|
F

P
|
P

P

P

L
|
L

A

A

N

G

N
x
H

-

V

-

A

Q

E

S
x
I

I

L

Q

A

M

K

P

E

S

G

A

G

V

R

N

E

P
x
Y

I

L

R

I

M

L

V
|
V

L
|
L

N

L

G

Y

G

G

Y
x
L

P
x
Q

P
x
G

S

Q

T
x
I

D

E

A

V

N

K

P

G

H

M

P

K

Y
|
Y

G
x
N

-
x
G

-
x
V

M
|
M

P

S

F
|
F

A

A

Q

Q

S

-

L

L

S

K

N

D

Q

E

E

E

V

I

A

A

V

V

V

L

T

N

Y

H

I
|
I

R

A

M

T

S

A

W

W

G

G

N

D

binding 2-acetyl-protoporphyrin ix: Y7 (= Y330), C10 (= C334), C13 (= C337), H14 (= H338), F25 (= F349), P26 (= P350), L28 (= L352), H31 (≠ N355), I35 (≠ S357), Y44 (≠ P366), V48 (= V370), L49 (= L371), L53 (≠ Y375), Q54 (≠ P376), G55 (≠ P377), I57 (≠ T379), Y64 (= Y386), N65 (≠ G387), G66 (vs. gap), V67 (vs. gap), M68 (= M388), F71 (= F391), I86 (= I407)

Sites not aligning to the query:

binding 2-acetyl-protoporphyrin ix: 124

1c52A Thermus thermophilus cytochrome-c552: a new highly thermostable cytochromE-C structure obtained by mad phasing (see paper)
38% identity, 20% coverage: 326:413/432 of query aligns to 4:93/131 of 1c52A

query
sites
1c52A

G

G

Q

A

K

K

I

I

Y

Y

A

A

D

-

N

Q

C
|
C

A

A

K

G

C
|
C

H
|
H

A

Q

D

Q

N

N

G

G

L

Q

G

G

Q

I

P

P

P

G

A

A

F
|
F

P
|
P

P

P

L
|
L

A

A

N

G

N
x
H

-

V

-

A

Q

E

S
x
I

I

L

Q

A

M

K

P

E

S

G

A

G

V

R

N

E

P
x
Y

I

L

R

I

M

L

V
|
V

L
|
L

N

L

G

Y

G

G

Y
x
L

P
x
Q

P
x
G

S

Q

T
x
I

D

E

A

V

N

K

P

G

H

M

P

K

Y
|
Y

G
x
N

-
x
G

-
x
V

M
|
M

P

S

F
|
F

A

A

Q

Q

S

-

L

L

S

K

N

D

Q

E

E

E

V

I

A

A

V

V

V

L

T

N

Y

H

I
|
I

R

A

M

T

S

A

W

W

G

G

N

D

binding protoporphyrin ix containing fe: C11 (= C334), C14 (= C337), H15 (= H338), F26 (= F349), P27 (= P350), L29 (= L352), H32 (≠ N355), I36 (≠ S357), Y45 (≠ P366), V49 (= V370), L50 (= L371), L54 (≠ Y375), Q55 (≠ P376), G56 (≠ P377), I58 (≠ T379), Y65 (= Y386), N66 (≠ G387), G67 (vs. gap), V68 (vs. gap), M69 (= M388), F72 (= F391), I87 (= I407)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 125

Query Sequence

>H281DRAFT_04220 H281DRAFT_04220 Cytochrome c, mono- and diheme variants
MKRKSLFALSAVVVVATAALVPVLWSGGDNLHNGSAVAATPADQAALVKKGEYLARAGDC
IACHTVRGGKQFAGGLPMATPFGTMFTPNITPDDQYGIGKWTQDDFYRAMHTGRSKDGSL
LYPGFPFTSYTKVTRADSDAIYAYLRSVTPVNVASRPHELKFPFNQRNMLIGWRTLFFRE
GEYKADPTKSVEWNRGAYLIEGLGHCGMCHTSINAMGGPVSSAAFAGGLIPLQNWYAPSL
TSNKEAGLGDWETKDIADLLKTGVSNRGAVFGPMAEVVHNSLQYMTDADINAMATYLKTI
PQKSEAPEPLQLETSEKFGGELLKQGQKIYADNCAKCHADNGLGQPPAFPPLANNQSIQM
PSAVNPIRMVLNGGYPPSTDANPHPYGMPPFAQSLSNQEVAAVVTYIRMSWGNHGTAVSP
QQVSDLRSAPLD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory