SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_04666 FitnessBrowser__Burk376:H281DRAFT_04666 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
29% identity, 40% coverage: 32:201/425 of query aligns to 56:207/444 of Q8NLB7

query
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Q8NLB7

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D57 (= D33) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R85) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
23% identity, 88% coverage: 14:389/425 of query aligns to 25:406/448 of Q51955

query
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D41 (≠ E30) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D33) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (= G80) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (= D84) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G87) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R127) mutation to A: Abolishes 4-HBA transport.
E144 (= E147) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ R193) mutation to A: Decrease in 4-HBA transport and chemotaxis.
D323 (= D298) mutation to N: Abolishes 4-HBA transport and chemotaxis.
H328 (≠ T303) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.
R386 (= R364) mutation to A: Strong decrease in 4-HBA transport.
R398 (≠ G381) mutation to A: Abolishes 4-HBA transport.

Sites not aligning to the query:

444 H→A: No change in 4-HBA transport and chemotaxis.

P36035 Carboxylic acid transporter protein homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
24% identity, 80% coverage: 37:374/425 of query aligns to 154:499/616 of P36035

query
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P36035

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K338 (≠ S226) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
9 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Query Sequence

>H281DRAFT_04666 FitnessBrowser__Burk376:H281DRAFT_04666
MNATTAASLAGSRRNSWRAVIAASIGNALEWFDLVVYGFFAVVIAKLFFPAGNDTVSLLL
TLGTFGVSFFMRPLGAIVIGAYADRAGRKAALTLSILLMMGGTLIIAVLPTYRSIGIAAP
VILVIARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQVASQGLTTLLAAGFGVALTG
NLTPDQMASWGWRVPFFFGLLIGPVAWYIRTKLDETPEFLAAETTSTPLRDTFTSQKLRL
LIAIGAVVLGTVSTYLVLFMPTFGVKQLGLAPSVAFSAIALTGLIQMVFAPVVGHLSDRH
GRTTIMLISAVLLLVLIYPAFVYLVAHPTFGTLIALQVVLGFLMTGYFAALPGLLSEMFP
VQTRTTGMSLAYNIAVTVFGGFGPFIIAWLIGFTGSKAAPSYYLIFAALLSVAALIAARR
KLGFR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory