SitesBLAST
Comparing H281DRAFT_04686 FitnessBrowser__Burk376:H281DRAFT_04686 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3ozwA The crystal structure of flavohemoglobin from r. Eutrophus in complex with ketoconazole (see paper)
54% identity, 98% coverage: 4:396/401 of query aligns to 2:397/403 of 3ozwA
- binding flavin-adenine dinucleotide: A46 (≠ T48), Q50 (≠ S52), R206 (= R203), Q207 (= Q204), Y208 (= Y205), S209 (= S206), S222 (= S219), V223 (= V220), K224 (= K221), E226 (= E223), P232 (≠ A230), G234 (= G232), Y235 (≠ H233), V236 (= V234), S237 (= S235), V276 (= V274), T279 (= T277), V395 (= V394), F396 (= F395)
- binding protoporphyrin ix containing fe: V42 (≠ L44), F43 (= F45), I81 (= I83), H85 (= H87), L88 (= L90), V90 (≠ I92), Q94 (≠ H96), Y95 (= Y97), V98 (= V100), Y126 (= Y128), A130 (= A132), L133 (≠ F135)
- binding 1-acetyl-4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazine: Q53 (= Q55), A56 (≠ T58), L102 (= L104)
Sites not aligning to the query:
3ozvA The crystal structure of flavohemoglobin from r. Eutrophus in complex with econazole (see paper)
54% identity, 98% coverage: 4:396/401 of query aligns to 2:397/403 of 3ozvA
- binding 1-[(2s)-2-[(4-chlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1h-imidazole: I24 (= I26), F28 (= F30), L57 (= L59), L102 (= L104), I106 (= I108)
- binding flavin-adenine dinucleotide: N44 (= N46), A46 (≠ T48), Q48 (= Q50), R206 (= R203), Q207 (= Q204), Y208 (= Y205), S209 (= S206), S222 (= S219), V223 (= V220), K224 (= K221), E226 (= E223), P232 (≠ A230), G234 (= G232), Y235 (≠ H233), V236 (= V234), S237 (= S235), V276 (= V274), T279 (= T277), F396 (= F395)
- binding protoporphyrin ix containing fe: F43 (= F45), I81 (= I83), K84 (= K86), H85 (= H87), L88 (= L90), V90 (≠ I92), Q94 (≠ H96), Y95 (= Y97), V98 (= V100), Y126 (= Y128), A130 (= A132), L133 (≠ F135)
3ozuA The crystal structure of flavohemoglobin from r. Eutrophus in complex with miconazole (see paper)
54% identity, 98% coverage: 4:396/401 of query aligns to 2:397/403 of 3ozuA
- binding flavin-adenine dinucleotide: A46 (≠ T48), H47 (= H49), R206 (= R203), Q207 (= Q204), Y208 (= Y205), S209 (= S206), S222 (= S219), V223 (= V220), K224 (= K221), E226 (= E223), P232 (≠ A230), G234 (= G232), Y235 (≠ H233), V236 (= V234), S237 (= S235), E394 (= E393), V395 (= V394), G397 (= G396)
- binding protoporphyrin ix containing fe: F43 (= F45), N44 (= N46), I81 (= I83), H85 (= H87), L88 (= L90), V90 (≠ I92), Q94 (≠ H96), Y95 (= Y97), V98 (= V100), Y126 (= Y128), L129 (= L131), A130 (= A132), L133 (≠ F135)
- binding 1-[(2R)-2-[(2,4-dichlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1H-imidazole: I25 (≠ T27), F28 (= F30), F43 (= F45), A56 (≠ T58), L57 (= L59), L102 (= L104), W122 (= W124), A125 (= A127), Y126 (= Y128)
Sites not aligning to the query:
P39662 Flavohemoprotein; FHP; Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (Ralstonia eutropha) (see paper)
54% identity, 98% coverage: 4:396/401 of query aligns to 2:397/403 of P39662
- A60 (= A62) mutation to Y: Does not affect phospholipid-binding.
- V98 (= V100) mutation to F: Blocks phospholipid-binding.
1cqxA Crystal structure of the flavohemoglobin from alcaligenes eutrophus at 1.75 a resolution (see paper)
54% identity, 98% coverage: 4:396/401 of query aligns to 2:397/403 of 1cqxA
- binding flavin-adenine dinucleotide: A46 (≠ T48), H47 (= H49), Y190 (= Y187), R206 (= R203), Q207 (= Q204), Y208 (= Y205), S209 (= S206), S222 (= S219), E226 (= E223), Q231 (≠ D228), P232 (≠ A230), G234 (= G232), Y235 (≠ H233), V236 (= V234), S237 (= S235), V395 (= V394), G397 (= G396)
- binding protoporphyrin ix containing fe: F43 (= F45), N44 (= N46), I81 (= I83), H85 (= H87), L88 (= L90), V90 (≠ I92), Q94 (≠ H96), Y95 (= Y97), V98 (= V100), Y126 (= Y128), L129 (= L131), A130 (= A132), L133 (≠ F135)
Sites not aligning to the query:
1gvhA The x-ray structure of ferric escherichia coli flavohemoglobin reveals an unespected geometry of the distal heme pocket (see paper)
43% identity, 98% coverage: 4:396/401 of query aligns to 2:391/396 of 1gvhA
- binding flavin-adenine dinucleotide: S46 (≠ T48), Q48 (= Q50), N50 (≠ S52), Y188 (= Y187), R204 (= R203), Q205 (= Q204), Y206 (= Y205), S207 (= S206), A220 (≠ S219), V221 (= V220), E224 (= E223), G227 (= G232), Q228 (≠ H233), V229 (= V234), S230 (= S235), V269 (= V274), T272 (= T277), E388 (= E393), F390 (= F395)
- binding protoporphyrin ix containing fe: F43 (= F45), Q53 (= Q55), A56 (≠ T58), L57 (= L59), A60 (= A62), I61 (≠ V63), I81 (= I83), K84 (= K86), H85 (= H87), I90 (= I92), Q94 (≠ H96), Y95 (= Y97), L127 (= L131), F131 (= F135)
Sites not aligning to the query:
P24232 Flavohemoprotein; Flavohemoglobin; HMP; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Escherichia coli (strain K12) (see 2 papers)
43% identity, 98% coverage: 4:396/401 of query aligns to 2:391/396 of P24232
- Y29 (= Y31) mutation Y->E,H: 15 to 35-fold reduction in NO dioxygenase activity.; mutation to F: 30-fold reduction in NO dioxygenase activity, and 80-fold increase in the O(2) dissociation rate constant.
- Y95 (= Y97) active site, Charge relay system
- E135 (= E139) active site, Charge relay system
4g1bA X-ray structure of yeast flavohemoglobin in complex with econazole (see paper)
38% identity, 96% coverage: 12:396/401 of query aligns to 10:391/398 of 4g1bA
- binding 1-[(2s)-2-[(4-chlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1h-imidazole: F28 (= F30), Y29 (= Y31), A56 (≠ T58), L57 (= L59), T60 (≠ A62), L102 (= L104), Y126 (= Y128)
- binding flavin-adenine dinucleotide: T46 (= T48), V50 (≠ S52), K84 (= K86), Y189 (= Y187), R207 (= R203), H208 (≠ Q204), Y209 (= Y205), S210 (= S206), A223 (≠ S219), K225 (= K221), E227 (= E223), F233 (≠ I229), P234 (≠ A230), G236 (= G232), L237 (≠ H233), V238 (= V234), S239 (= S235), V282 (= V274), F390 (= F395)
- binding protoporphyrin ix containing fe: I42 (≠ L44), F43 (= F45), N44 (= N46), N47 (≠ H49), T60 (≠ A62), Q80 (≠ R82), I81 (= I83), K84 (= K86), H85 (= H87), L88 (= L90), I90 (= I92), H94 (= H96), Y95 (= Y97), V98 (= V100), F133 (= F135)
Sites not aligning to the query:
Q03331 Flavohemoprotein; Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Candida norvegensis (Yeast) (Candida mycoderma) (see paper)
33% identity, 99% coverage: 1:396/401 of query aligns to 10:384/390 of Q03331
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylserine
6o0aA Crystal structure of flavohemoglobin from malassezia yamatoensis with bound fad and heme determined by iron sad phasing (see paper)
32% identity, 96% coverage: 12:396/401 of query aligns to 11:383/383 of 6o0aA
- binding flavin-adenine dinucleotide: S48 (≠ T48), R51 (≠ Q51), D52 (≠ S52), Y190 (= Y187), R205 (= R203), Q206 (= Q204), F207 (≠ Y205), T208 (≠ S206), K222 (= K221), D224 (≠ A230), H226 (≠ G232), G227 (≠ H233), E228 (≠ V234), M229 (≠ S235), T230 (≠ N236), T274 (= T277), E380 (= E393), F382 (= F395), G383 (= G396)
- binding protoporphyrin ix containing fe: M44 (≠ L44), F45 (= F45), S46 (≠ N46), L59 (= L59), S62 (≠ A62), R84 (= R82), V85 (≠ I83), H89 (= H87), L92 (= L90), L94 (≠ I92), E98 (≠ H96), Y99 (= Y97), V102 (= V100), Y130 (= Y128), L133 (= L131)
4eh1A Crystal structure of the flavohem-like-fad/NAD binding domain of nitric oxide dioxygenase from vibrio cholerae o1 biovar el tor
42% identity, 61% coverage: 152:396/401 of query aligns to 4:236/237 of 4eh1A
- binding flavin-adenine dinucleotide: Y39 (= Y187), R55 (= R203), Q56 (= Q204), Y57 (= Y205), S58 (= S206), S71 (= S219), V72 (= V220), E75 (= E223), N81 (≠ A230), G83 (= G232), L84 (≠ H233), V85 (= V234), S86 (= S235), T127 (= T277), E233 (= E393), F235 (= F395)
3tm9A Y29a mutant of vitreoscilla stercoraria hemoglobin (see paper)
52% identity, 36% coverage: 4:146/401 of query aligns to 2:144/146 of 3tm9A
- binding protoporphyrin ix containing fe: L42 (= L44), F43 (= F45), Q53 (= Q55), L57 (= L59), T60 (≠ A62), I81 (= I83), K84 (= K86), H85 (= H87), V90 (≠ I92), Y95 (= Y97), V98 (= V100), Y126 (= Y128), F133 (= F135)
4vhbA Thiocyanate adduct of the bacterial hemoglobin from vitreoscilla sp. (see paper)
51% identity, 36% coverage: 4:146/401 of query aligns to 2:136/138 of 4vhbA
- binding protoporphyrin ix containing fe: L42 (= L44), F43 (= F45), L49 (= L59), T52 (≠ A62), I73 (= I83), K76 (= K86), H77 (= H87), V82 (≠ I92), H86 (= H96), Y87 (= Y97), V90 (= V100), F125 (= F135)
- binding thiocyanate ion: F28 (= F30), L49 (= L59)
3vhbA Imidazole adduct of the bacterial hemoglobin from vitreoscilla sp. (see paper)
51% identity, 36% coverage: 4:146/401 of query aligns to 1:134/135 of 3vhbA
- binding protoporphyrin ix containing fe: F42 (= F45), L47 (= L59), T50 (≠ A62), I71 (= I83), K74 (= K86), H75 (= H87), V80 (≠ I92), H84 (= H96), Y85 (= Y97), V88 (= V100), Y116 (= Y128), F123 (= F135)
- binding imidazole: Y28 (= Y31), L47 (= L59)
2wy4A Structure of bacterial globin from campylobacter jejuni at 1.35 a resolution (see paper)
45% identity, 35% coverage: 5:144/401 of query aligns to 1:138/139 of 2wy4A
- binding protoporphyrin ix containing fe: M40 (≠ L44), F41 (= F45), N42 (= N46), Q51 (= Q55), L55 (= L59), A58 (= A62), V79 (≠ I83), T82 (≠ K86), H83 (= H87), L86 (= L90), V88 (≠ I92), H92 (= H96), Y93 (= Y97), V96 (= V100), Y129 (≠ F135)
7dihA Crystal structure of thermoglobin y29f mutant in complex with imidazole
43% identity, 35% coverage: 4:143/401 of query aligns to 2:136/139 of 7dihA
- binding protoporphyrin ix containing fe: L42 (= L44), F43 (= F45), A46 (≠ T48), S47 (= S52), Q50 (= Q55), L54 (= L59), I78 (= I83), S81 (≠ K86), H82 (= H87), V87 (≠ I92), H91 (= H96), Y92 (= Y97), V95 (= V100), Y121 (= Y128), L124 (= L131)
6wk3D Engineered carbene transferase rmanod q52v, putative nitric oxide dioxygenase from rhodothermus marinus (see paper)
36% identity, 33% coverage: 12:143/401 of query aligns to 14:141/146 of 6wk3D
- binding copper (ii) ion: E51 (≠ H49), H55 (≠ G53)
- binding protoporphyrin ix containing fe: L46 (= L44), F47 (= F45), L57 (= L59), R80 (= R82), M81 (≠ I83), S84 (≠ K86), H85 (= H87), R87 (≠ S89), A88 (≠ L90), V90 (≠ I92), H94 (= H96), Y95 (= Y97), V98 (= V100), Y126 (= Y128)
1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
27% identity, 60% coverage: 156:397/401 of query aligns to 112:336/337 of 1krhA
- active site: C306 (= C367)
- binding flavin-adenine dinucleotide: Y143 (= Y187), R155 (= R203), S156 (≠ Q204), Y157 (= Y205), S158 (= S206), V171 (≠ S219), V172 (= V220), G178 (= G232), K179 (≠ H233), M180 (≠ V234), S181 (= S235), T219 (≠ V274), E332 (= E393), F334 (= F395), S335 (≠ G396), A336 (≠ S397)
Sites not aligning to the query:
P33164 Phthalate dioxygenase reductase; PDR; EC 1.-.-.- from Burkholderia cepacia (Pseudomonas cepacia) (see paper)
34% identity, 40% coverage: 150:309/401 of query aligns to 8:156/322 of P33164
Sites not aligning to the query:
- 226 binding
- 273 binding
- 275 binding
- 278 binding
- 281 binding
- 309 binding
2piaA Phthalate dioxygenase reductase: a modular structure for electron transfer from pyridine nucleotides to [2fe-2s] (see paper)
34% identity, 40% coverage: 150:309/401 of query aligns to 7:155/321 of 2piaA
- binding flavin mononucleotide: N44 (≠ Y187), R55 (= R203), T56 (≠ Q204), Y57 (= Y205), S58 (= S206), A72 (≠ S219), V73 (= V220), G79 (= G225), R80 (= R226), G82 (= G232), S83 (≠ H233), I121 (≠ V274), T124 (= T277)
Sites not aligning to the query:
Query Sequence
>H281DRAFT_04686 FitnessBrowser__Burk376:H281DRAFT_04686
MTALTAEQIARVKATVPVLAEHGTTITKHFYKRMFARHPELKNLFNQTHQQSGNQPETLA
RAVYAYAANIDNLGALGPTVSRIANKHASLNIRPEHYPIVGRHLLGAIVDVLGDAVDQPT
LDAWQVAYGQLANIFVGTEAKLYEGAAWSGFRPFKVARKQVESDEITSFYLTPADGSAAC
DFEPGQYVSVKRFVDKLGVDQPRQYSLSDAPHGKWLRISVKREDGREDIAPGHVSNLLHA
DVEEGTVVHVSAPMGDFTLDRKKTTPVVLMSGGVGVTPMTSMLSTILADGSERSVTFVHA
CRNGRVHAFRQWLNETVASHSNVKRVVFYEAVGEGDRRGVDYDFDGRLDVAGIAGQVIVP
DADYYICGPVPFMRAQCDALAALGVDPARIHTEVFGSGVVE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory