SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_04726 FitnessBrowser__Burk376:H281DRAFT_04726 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4n0wA X-ray crystal structure of a serine hydroxymethyltransferase from burkholderia cenocepacia with covalently attached pyridoxal phosphate
94% identity, 100% coverage: 1:414/415 of query aligns to 2:415/416 of 4n0wA

query
sites
4n0wA

M

M

F

F

D

D

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R

A

A

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Q

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active site: Y57 (= Y56), E59 (= E58), D202 (= D201), T228 (= T227), K231 (= K230), R237 (= R236)
binding pyridoxal-5'-phosphate: S99 (= S98), G100 (= G99), S101 (= S100), H128 (= H127), D202 (= D201), A204 (= A203), H205 (= H204), K231 (= K230)

4ot8A X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and serine
94% identity, 100% coverage: 2:414/415 of query aligns to 1:413/414 of 4ot8A

query
sites
4ot8A

F

F

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D

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active site: Y55 (= Y56), E57 (= E58), D200 (= D201), T226 (= T227), K229 (= K230), R235 (= R236)
binding pyridoxal-5'-phosphate: S97 (= S98), G98 (= G99), S99 (= S100), H126 (= H127), D200 (= D201), A202 (= A203), H203 (= H204), K229 (= K230)
binding serine: S35 (= S36), E57 (= E58), Y65 (= Y66), H126 (= H127), H203 (= H204), R360 (= R361)

4otlA X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and glycine
94% identity, 98% coverage: 7:414/415 of query aligns to 1:408/409 of 4otlA

query
sites
4otlA

S

S

T

T

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I

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A

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N

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V

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D

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E

L

I

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H

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M

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Y

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G

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C

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A

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A

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G

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L

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Y

active site: Y50 (= Y56), E52 (= E58), D195 (= D201), T221 (= T227), K224 (= K230), R230 (= R236)
binding glycine: S30 (= S36), Y50 (= Y56), Y60 (= Y66), H121 (= H127), K224 (= K230), R355 (= R361)
binding pyridoxal-5'-phosphate: S92 (= S98), G93 (= G99), S94 (= S100), H121 (= H127), S170 (= S176), D195 (= D201), A197 (= A203), H198 (= H204), K224 (= K230)

1dfoB Crystal structure at 2.4 angstrom resolution of e. Coli serine hydroxymethyltransferase in complex with glycine and 5-formyl tetrahydrofolate (see paper)
64% identity, 99% coverage: 4:414/415 of query aligns to 3:416/417 of 1dfoB

query
sites
1dfoB

R

K

A

R

Q

E

S

M

T

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D

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A

E

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E

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Q

E

E

H

H

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I

E

E

L

L

I

I

A

A

S
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S

E

E

N

N

Y

Y

T

T

S

S

P

P

A

R

V

V

M

M

A

Q

A

A

Q

Q

G

G

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S

Q

Q

L

L

T

T

N

N

K

K

Y
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Y

A

A

E
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E

G

G

Y

Y

P

P

G

G

K

K

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Y
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Y

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G

C

C

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E

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D

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A

V

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E

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A

A

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I

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D

R

R

V

A

K

K

Q

E

L

L

F

F

G

G

A

A

E

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A

Y

A

A

N

N

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V

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N

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S

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G

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S

Q

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A

A

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F

G

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L

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E

P

P

G

G

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D

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T

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L

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G

H
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H

L
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L

T

T

H

H

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G

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S

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P

V

V

N

N

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F

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S

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G

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x
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S

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A

G

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Y

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N

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K

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R

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-

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-
x
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-

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E

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N

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S

A

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G
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G

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A

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G

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A

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A

A

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E

P

P

E

E

F

F

K

K

A

T

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Y

Q

Q

V

V

V

A

E

K

N

N

A

A

R

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A

L

M

A

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F

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L

K

E

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R

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G

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Y

R

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G

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G

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T

E

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N

H

H

V

L

M

F

L

L

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V

D

D

L

L

R

V

A

D

K

K

K

N

I

L

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T

G

G

K

K

A

E

A

A

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D

A

A

L

L

G

G

A

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A

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N

I

I

T

T

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V

N

N

K

K

N
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N

A

S

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P

N

N

D

D

P

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E

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K
x
S

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P

F
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F

V

V

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T

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S

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R

L

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A

A

M

I

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T

R

R

R

G

G

F

F

G

K

T

E

K

A

E

E

A

A

E

K

Q

E

V

L

G

A

N

G

L

W

I

M

A

C

D

D

V

V

L

L

D

D

N

S

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I

E

N

D

D

T

E

A

A

T

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I

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E

R

R

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R

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G

G

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K

V

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Y

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P

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V

Y

Y

active site: Y55 (= Y56), E57 (= E58), D200 (= D201), T226 (= T227), K229 (= K230), R235 (= R236)
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E57 (= E58), Y64 (= Y65), Y65 (= Y66), L121 (= L122), G125 (= G126), H126 (= H127), L127 (= L128), S175 (= S176), S245 (vs. gap), E247 (= E245), N347 (= N345), S355 (≠ K353), P356 (= P354), F357 (= F355)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: S35 (= S36), Y55 (= Y56), Y65 (= Y66), S97 (= S98), G98 (= G99), S99 (= S100), H126 (= H127), F174 (≠ A175), S175 (= S176), D200 (= D201), A202 (= A203), H203 (= H204), K229 (= K230), G262 (= G260), R363 (= R361)

P0A825 Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1 from Escherichia coli (strain K12) (see 8 papers)
64% identity, 99% coverage: 4:414/415 of query aligns to 3:416/417 of P0A825

query
sites
P0A825

R

K

A

R

Q

E

S

M

T

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A

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D

D

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A

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Q

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E

H

H

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E

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L

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Y

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S

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P

A

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V

M

M

A

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A

A

Q

Q

G

G

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S

Q

Q

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L

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T

N

N

K
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K

Y
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Y

A

A

E

E

G

G

Y

Y

P

P

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G

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K

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G

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Y

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D

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K

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L

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F

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G

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Y

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A

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N

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V

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Q

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E

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P

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N

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G

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K

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N

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A

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K

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x
K

K

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P

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x
K

T

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K

A

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E

A

A

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K

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E

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L

G

A

N

G

L

W

I

M

A

C

D

D

V

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L

D

D

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D

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A

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V

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Y

K54 (= K55) modified: N6-acetyllysine
Y55 (= Y56) mutation to F: 50 and 15-fold increase in the affinity for serine and tetrahydrofolate, respectively, and 4-fold decrease in the catalytic efficiency.
K62 (= K63) modified: N6-succinyllysine
Y65 (= Y66) mutation to F: Decrease in catalytic activity.
L85 (= L86) mutation to A: Alteration of the dimer-monomer equilibrium accompanied by minor changes in the catalytic properties and whitout any significant change of tertiary structure. In the monomeric state; when associated with A-276.
P214 (= P215) mutation to A: No significant difference in catalytic efficiency and affinity compared to the wild-type.; mutation to G: No significant difference in catalytic efficiency and affinity compared to the wild-type.
P216 (= P217) mutation to A: No significant difference in catalytic efficiency and affinity compared to the wild-type. Alteration in the folding rate.; mutation to G: Important decrease in affinity and catalytic efficiency. Severely compromised in folding into a catalytically competent enzyme.
P218 (= P219) mutation to A: No significant difference in catalytic efficiency and affinity compared to the wild-type.; mutation to G: No significant difference in catalytic efficiency and affinity compared to the wild-type.
H228 (= H229) Plays an important role in substrate specificity; binding ; mutation H->D,N: Utilize substrates and substrate analogs more effectively for a variety of alternate non-physiological reactions.
K229 (= K230) modified: N6-(pyridoxal phosphate)lysine
R235 (= R236) binding ; mutation to K: 1500- and 20-fold increase in the affinity for serine and tetrahydrofolate, respectively, and 15-fold decrease in the catalytic efficiency.; mutation to L: 450- and 11-fold increase in the affinity for serine and tetrahydrofolate, respectively, and 60-fold decrease in the catalytic efficiency.; mutation to Q: 900- and 17-fold increase in the affinity for serine and tetrahydrofolate, respectively, and 30-fold decrease in the catalytic efficiency.
K242 (= K243) modified: N6-succinyllysine
K250 (= K248) modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate
P258 (= P256) mutation to A: Important decrease in affinity and catalytic efficiency. Reduced thermal stability.; mutation to G: Important decrease in affinity and catalytic efficiency.
P264 (= P262) mutation to A: Important decrease in affinity and catalytic efficiency.; mutation to G: Important decrease in affinity and catalytic efficiency.
L276 (≠ F274) mutation to A: Alteration of the dimer-monomer equilibrium accompanied by minor changes in the catalytic properties and whitout any significant change of tertiary structure. In the monomeric state; when associated with A-85.
K277 (= K275) modified: N6-succinyllysine
K285 (= K283) modified: N6-acetyllysine
K293 (≠ E291) modified: N6-succinyllysine
K331 (= K329) modified: N6-succinyllysine
K346 (= K344) modified: N6-succinyllysine
K354 (≠ E352) modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate
R363 (= R361) mutation to A: It does not bind serine and glycine and shows no activity with serine as the substrate.; mutation to K: Exhibits only 0.03% of the catalytic activity of the wild-type and a 15-fold reduction in affinity for glycine and serine.
R372 (= R370) mutation to A: No significant difference compared to the wild-type.; mutation to K: No significant difference compared to the wild-type.
K375 (≠ G373) modified: N6-acetyllysine

1eqbA X-ray crystal structure at 2.7 angstroms resolution of ternary complex between the y65f mutant of e-coli serine hydroxymethyltransferase, glycine and 5-formyl tetrahydrofolate (see paper)
64% identity, 99% coverage: 4:414/415 of query aligns to 2:415/416 of 1eqbA

query
sites
1eqbA

R

K

A

R

Q

E

S

M

T

N

I

I

A

A

N

D

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D

D

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A

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E

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L

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W

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E

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E

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R

Q

Q

E

E

H

H

I

I

E

E

L

L

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A

A

S
|
S

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E

N

N

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Y

T

T

S

S

P

P

A

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V

M

M

A

Q

A

A

Q

Q

G

G

S

S

Q

Q

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

K

R

R

Y
|
Y

Y
x
F

G

G

C

C

E

E

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Y

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V

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D

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V

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E

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L

A

A

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I

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D

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R

V

A

K

K

Q

E

L

L

F

F

G

G

A

A

E

D

A

Y

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

Q

F

G

A

V

V

F

Y

F

T

A

A

M

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L

L

K

E

P

P

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G

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D

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G

G

M

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N

L
|
L

A

A

H

H

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

K

W

L

F

Y

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N

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Y

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G

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E

E

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H

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K

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P

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K

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I

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I

A

G

G

G

A
x
F

S
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S

A

A

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G

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A

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Y

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D
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D

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M

A

A

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H

Y

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A

A

G

G

L

L

I

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A

A

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G

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V

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Y

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P

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N

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P

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P

H

H

A

A

D

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T
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T

T

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R

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A

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-

G

-

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-

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P

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E

F

F

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K

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Y

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E

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N

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F

L

L

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D

L

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D

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G

K

K

A

E

A

A

E

D

A

A

L

L

G

G

A

R

A

A

H

N

I

I

T

T

V

V

N

N

K

K

N
|
N

A

S

I

V

P

P

N

N

D

D

P

P

E

K

K
x
S

P
|
P

F
|
F

V

V

T

T

S

S

G

G

I

I

R
|
R

L

V

G

G

S

T

P

P

A

A

M

I

T

T

T

R

R

R

G

G

F

F

G

K

T

E

K

A

E

E

A

A

E

K

Q

E

V

L

G

A

N

G

L

W

I

M

A

C

D

D

V

V

L

L

D

D

N

S

P

I

E

N

D

D

T

E

A

A

T

V

I

I

D

E

R

R

V

I

R

K

G

G

Q

K

V

V

A

L

E

D

L

I

T

C

Q

A

R

R

F

Y

P

P

V

V

Y

Y

active site: Y54 (= Y56), E56 (= E58), D199 (= D201), T225 (= T227), K228 (= K230), R234 (= R236)
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E56 (= E58), Y63 (= Y65), L120 (= L122), G123 (= G125), G124 (= G126), H125 (= H127), L126 (= L128), S174 (= S176), N346 (= N345), S354 (≠ K353), P355 (= P354), F356 (= F355)
binding glycine: S34 (= S36), Y54 (= Y56), F64 (≠ Y66), H202 (= H204), K228 (= K230), R362 (= R361)
binding pyridoxal-5'-phosphate: S96 (= S98), G97 (= G99), S98 (= S100), H125 (= H127), F173 (≠ A175), S174 (= S176), D199 (= D201), H202 (= H204), H227 (= H229), K228 (= K230)

6ymfA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the plp-serine external aldimine state (see paper)
60% identity, 98% coverage: 9:414/415 of query aligns to 7:414/418 of 6ymfA

query
sites
6ymfA

I

L

A

L

N

Q

V

T

D

D

P

P

E

T

L

I

W

S

K

G

V

M

I

M

E

Q

Q

K

E

E

N

L

R

Q

R

R

Q

Q

E

R

E

E

H

H

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

Y

F

T

T

S

S

P

P

A

A

V

V

M

M

A

A

A

T

Q

Q

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

L

P

P

G

K

K

K

R

R

Y

Y

G

G

C

C

E

E

Y

F

V

I

D

D

V

E

A

I

E

E

Q

Q

L

V

A

A

I

I

D

D

R

R

V

A

K

K

Q

E

L

L

F

F

G

G

A

A

E

A

A

S

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

Q

F

G

A

V

V

F

F

F

L

A

T

M

L

L

L

K

K

P

P

G

G

D

D

T

K

I

I

M

M

G

G

M

M

S

D

L

L

A

S

H

H

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

A

N

N

M

V

S

S

G

G

K

K

W

W

F

F

N

E

V

A

V

V

S

H

Y

Y

G

G

L

V

N

S

-

Q

E

E

A

T

E

E

D

Q

I

L

D

D

Y

Y

D

D

A

H

A

I

E

L

K

E

L

V

A

A

Q

R

E

Q

H

E

K

R

P

P

K

K

L

L

I

I

V

I

A

C

G

G

A

Y

S
|
S

A

A

F

Y

A

P

L

R

R

I

I

I

D

N

F

F

E

E

R

K

L

F

S

R

K

A

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

Y

F

L

M

L

V

A

D
|
D

M

I

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

A

S

G

G

V

H

Y

H

P

P

N

N

P

P

V

V

P

P

H

H

A

C

D

D

F

V

V

V

T

T

T
|
T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

V

L

I

I

L

L

M

T

K

R

-

D

A

P

E

E

F

L

E

G

K

K

Q

K

I

F

N

N

S

K

A

S

I

V

F

F

P

P

G

G

I

T

Q

Q

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

G

G

K

K

A

A

V

V

A

A

F

F

K

G

E

E

A

A

L

L

S

K

P

P

E

E

F

F

K

K

A

A

Y

Y

Q

S

Q

G

Q

Q

V

V

V

I

E

A

N

N

A

A

R

Q

V

A

L

M

A

A

E

Q

T

Q

L

L

V

Q

K

N

R

R

G

G

L

F

R

K

I

I

V

V

S

S

G

G

R

G

T

T

E

D

S

N

H

H

V

L

M

M

L

L

V

V

D

D

L

L

R

R

A

S

K

V

K

N

I

L

T

T

G

G

K

K

A

Q

A

A

E

D

A

Q

A

L

L

V

G

S

A

D

A

V

H

N

I

I

T

T

V

A

N

N

K

K

N

N

A

T

I

V

P

P

N

F

D

D

P

P

E

E

K

S

P

P

F

F

V

V

T

T

S

S

G

G

I

L

R
|
R

L

L

G

G

S

S

P

P

A

A

M

M

T

T

R

R

G

G

F

L

G

G

T

T

K

E

E

D

A

F

E

A

Q

E

V

I

G

A

N

N

L

I

I

I

A

A

D

D

V

R

L

L

D

Q

N

N

P

P

E

E

D

D

T

E

A

Q

T

V

I

K

D

Q

R

A

V

C

R

V

G

Q

Q

R

V

V

A

A

E

A

L

L

T

C

Q

E

R

R

F

F

P

P

V

L

Y

Y

active site: Y54 (= Y56), E56 (= E58), D200 (= D201), T226 (= T227), K229 (= K230), R235 (= R236)
binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S34 (= S36), S96 (= S98), G97 (= G99), A98 (≠ S100), H125 (= H127), S175 (= S176), D200 (= D201), A202 (= A203), H203 (= H204), T226 (= T227), K229 (= K230), R361 (= R361)

6ymdA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the covalent complex with malonate (see paper)
60% identity, 98% coverage: 9:414/415 of query aligns to 7:414/420 of 6ymdA

query
sites
6ymdA

I

L

A

L

N

Q

V

T

D

D

P

P

E

T

L

I

W

S

K

G

V

M

I

M

E

Q

Q

K

E

E

N

L

R

Q

R

R

Q

Q

E

R

E

E

H

H

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

Y

F

T

T

S

S

P

P

A

A

V

V

M

M

A

A

A

T

Q

Q

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

L

P

P

G

K

K

K

R

R

Y

Y

Y
|
Y

G

G

C

C

E

E

Y

F

V

I

D

D

V

E

A

I

E

E

Q

Q

L

V

A

A

I

I

D

D

R

R

V

A

K

K

Q

E

L

L

F

F

G

G

A

A

E

A

A

S

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

Q

F

G

A

V

V

F

F

F

L

A

T

M

L

L

L

K

K

P

P

G

G

D

D

T

K

I

I

M

M

G

G

M

M

S

D

L

L

A

S

H

H

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

A

N

N

M

V

S

S

G

G

K

K

W

W

F

F

N

E

V

A

V

V

S

H

Y

Y

G

G

L

V

N

S

-

Q

E

E

A

T

E

E

D

Q

I

L

D

D

Y

Y

D

D

A

H

A

I

E

L

K

E

L

V

A

A

Q

R

E

Q

H

E

K

R

P

P

K

K

L

L

I

I

V

I

A

C

G

G

A
x
Y

S
|
S

A

A

F

Y

A

P

L

R

R

I

I

I

D

N

F

F

E

E

R

K

L

F

S

R

K

A

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

Y

F

L

M

L

V

A

D
|
D

M

I

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

A

S

G

G

V

H

Y

H

P

P

N

N

P

P

V

V

P

P

H

H

A

C

D

D

F

V

V

V

T

T

T
|
T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

V

L

I

I

L

L

M

T

K

R

-

D

A

P

E

E

F

L

E

G

K

K

Q

K

I

F

N

N

S

K

A

S

I

V

F

F

P

P

G

G

I

T

Q

Q

G

G

G
|
G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

G

G

K

K

A

A

V

V

A

A

F

F

K

G

E

E

A

A

L

L

S

K

P

P

E

E

F

F

K

K

A

A

Y

Y

Q

S

Q

G

Q

Q

V

V

V

I

E

A

N

N

A

A

R

Q

V

A

L

M

A

A

E

Q

T

Q

L

L

V

Q

K

N

R

R

G

G

L

F

R

K

I

I

V

V

S

S

G

G

R

G

T

T

E

D

S

N

H

H

V

L

M

M

L

L

V

V

D

D

L

L

R

R

A

S

K

V

K

N

I

L

T

T

G

G

K

K

A

Q

A

A

E

D

A

Q

A

L

L

V

G

S

A

D

A

V

H

N

I

I

T

T

V

A

N

N

K

K

N

N

A

T

I

V

P

P

N

F

D

D

P

P

E

E

K

S

P

P

F

F

V

V

T

T

S

S

G

G

I

L

R
|
R

L

L

G

G

S

S

P

P

A

A

M

M

T

T

R

R

G

G

F

L

G

G

T

T

K

E

E

D

A

F

E

A

Q

E

V

I

G

A

N

N

L

I

I

I

A

A

D

D

V

R

L

L

D

Q

N

N

P

P

E

E

D

D

T

E

A

Q

T

V

I

K

D

Q

R

A

V

C

R

V

G

Q

Q

R

V

V

A

A

E

A

L

L

T

C

Q

E

R

R

F

F

P

P

V

L

Y

Y

active site: Y54 (= Y56), E56 (= E58), D200 (= D201), T226 (= T227), K229 (= K230), R235 (= R236)
binding malonate ion: S34 (= S36), Y54 (= Y56), E56 (= E58), Y64 (= Y66), H125 (= H127), H203 (= H204), K229 (= K230), R361 (= R361)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: Y54 (= Y56), S96 (= S98), G97 (= G99), A98 (≠ S100), H125 (= H127), Y174 (≠ A175), S175 (= S176), D200 (= D201), A202 (= A203), T226 (= T227), K229 (= K230), G261 (= G261)

1kl2A Crystal structure of serine hydroxymethyltransferase complexed with glycine and 5-formyl tetrahydrofolate (see paper)
62% identity, 95% coverage: 13:408/415 of query aligns to 8:404/405 of 1kl2A

query
sites
1kl2A

D

D

P

P

E

Q

L

V

W

F

K

A

V

A

I

I

E

E

Q

Q

E

E

N

R

R

K

R

R

Q

Q

E

H

E

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

Y

F

T

V

S

S

P

R

A

A

V

V

M

M

A

E

A

A

Q

Q

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y
|
Y

G

G

C

C

E

E

Y

Y

V

V

D

D

V

I

A

V

E

E

Q

E

L

L

A

A

I

R

D

E

R

R

V

A

K

K

Q

Q

L

L

F

F

G

G

A

A

E

E

A

H

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

Q

M

G

A

V

V

F

Y

F

F

A

T

M

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

S

N

L

L

A

S

H

H

G

G

G
|
G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

V

W

Q

F

Y

N

N

V

F

V

V

S

A

Y

Y

G

G

L

V

N

D

-

P

E

E

A

T

E

H

D

V

I

I

D

D

Y

Y

D

D

A

D

A

V

E

R

K

E

L

K

A

A

Q

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

V

V

A

A

G

A

A

A

S
|
S

A

A

F

Y

A

P

L

R

R

I

I

I

D

D

F

F

E

A

R

K

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

Y

F

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

P

H

Y

A

A

D

H

F

F

V

V

T

T

T
|
T

T

T

H
|
H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

V

M

I

I

L

L

M

C

K

Q

A

E

E

Q

F

F

E

A

K

K

Q

Q

I

I

N

D

S

K

A

A

I

I

F
|
F

P

P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

K

G

E

E

A

A

L

L

S

Q

P

D

E

D

F

F

K

K

A

A

Y

Y

Q

A

Q

K

Q

R

V

V

E

D

N

N

A

A

R

K

V

R

L

L

A

A

E

S

T

A

L

L

V

Q

K

N

R

E

G

G

L

F

R

T

I

L

V

V

S

S

G

G

R

G

T

T

E

D

S

N

H

H

V

L

M

L

L

L

V

V

D

D

L

L

R

R

A

P

K

Q

I

L

T

T

G

G

K

K

A

T

A

A

E

E

A

K

A

V

L

L

G

D

A

E

A

V

H

G

I

I

T

T

V

V

N

N

K

K

N
|
N

A

T

I

I

P

P

N

Y

D

D

P

P

E

E

K

S

P

P

F

F

V

V

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

S

T

P

A

A

A

M

V

T

T

R

R

G

G

F

F

G

G

T

L

K

E

E

E

A

M

E

D

Q

E

V

I

G

A

N

A

L

I

I

I

A

G

D

L

V

V

L

L

D

K

N

N

P

V

E

G

D

S

T

E

A

Q

T

A

I

L

D

E

R

E

V

A

R

R

G

Q

Q

R

V

V

A

A

E

A

L

L

T

T

active site: Y51 (= Y56), E53 (= E58), D197 (= D201), T223 (= T227), K226 (= K230), R232 (= R236)
binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: E53 (= E58), Y60 (= Y65), G121 (= G126), H122 (= H127), S172 (= S176), F251 (= F255), N341 (= N345)
binding glycine: S31 (= S36), Y51 (= Y56), Y61 (= Y66), H200 (= H204), R357 (= R361)
binding pyridoxal-5'-phosphate: S93 (= S98), G94 (= G99), A95 (≠ S100), H122 (= H127), S172 (= S176), D197 (= D201), A199 (= A203), H200 (= H204), T223 (= T227), H225 (= H229), K226 (= K230)

1kl1A Crystal structure of serine hydroxymethyltransferase complexed with glycine (see paper)
62% identity, 95% coverage: 13:408/415 of query aligns to 8:404/405 of 1kl1A

query
sites
1kl1A

D

D

P

P

E

Q

L

V

W

F

K

A

V

A

I

I

E

E

Q

Q

E

E

N

R

R

K

R

R

Q

Q

E

H

E

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

Y

F

T

V

S

S

P

R

A

A

V

V

M

M

A

E

A

A

Q

Q

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y

Y

G

G

C

C

E

E

Y

Y

V

V

D

D

V

I

A

V

E

E

Q

E

L

L

A

A

I

R

D

E

R

R

V

A

K

K

Q

Q

L

L

F

F

G

G

A

A

E

E

A

H

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

Q

M

G

A

V

V

F

Y

F

F

A

T

M

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

S

N

L

L

A

S

H

H

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

V

W

Q

F

Y

N

N

V

F

V

V

S

A

Y

Y

G

G

L

V

N

D

-

P

E

E

A

T

E

H

D

V

I

I

D

D

Y

Y

D

D

A

D

A

V

E

R

K

E

L

K

A

A

Q

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

V

V

A

A

G

A

A
|
A

S
|
S

A

A

F

Y

A

P

L

R

R

I

I

I

D

D

F

F

E

A

R

K

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

Y

F

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

P

H

Y

A

A

D

H

F

F

V

V

T

T

T
|
T

T

T

H
|
H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

V

M

I

I

L

L

M

C

K

Q

A

E

E

Q

F

F

E

A

K

K

Q

Q

I

I

N

D

S

K

A

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

K

G

E

E

A

A

L

L

S

Q

P

D

E

D

F

F

K

K

A

A

Y

Y

Q

A

Q

K

Q

R

V

V

E

D

N

N

A

A

R

K

V

R

L

L

A

A

E

S

T

A

L

L

V

Q

K

N

R

E

G

G

L

F

R

T

I

L

V

V

S

S

G

G

R

G

T

T

E

D

S

N

H

H

V

L

M

L

L

L

V

V

D

D

L

L

R

R

A

P

K

Q

I

L

T

T

G

G

K

K

A

T

A

A

E

E

A

K

A

V

L

L

G

D

A

E

A

V

H

G

I

I

T

T

V

V

N

N

K

K

N

N

A

T

I

I

P

P

N

Y

D

D

P

P

E

E

K

S

P

P

F

F

V

V

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

S

T

P

A

A

A

M

V

T

T

R

R

G

G

F

F

G

G

T

L

K

E

E

E

A

M

E

D

Q

E

V

I

G

A

N

A

L

I

I

I

A

G

D

L

V

V

L

L

D

K

N

N

P

V

E

G

D

S

T

E

A

Q

T

A

I

L

D

E

R

E

V

A

R

R

G

Q

Q

R

V

V

A

A

E

A

L

L

T

T

active site: Y51 (= Y56), E53 (= E58), D197 (= D201), T223 (= T227), K226 (= K230), R232 (= R236)
binding glycine: S31 (= S36), H122 (= H127), R357 (= R361)
binding pyridoxal-5'-phosphate: S93 (= S98), G94 (= G99), A95 (≠ S100), H122 (= H127), A171 (= A175), S172 (= S176), D197 (= D201), A199 (= A203), H200 (= H204), T223 (= T227), H225 (= H229), K226 (= K230)

1kkpA Crystal structure of serine hydroxymethyltransferase complexed with serine (see paper)
62% identity, 95% coverage: 13:408/415 of query aligns to 8:404/405 of 1kkpA

query
sites
1kkpA

D

D

P

P

E

Q

L

V

W

F

K

A

V

A

I

I

E

E

Q

Q

E

E

N

R

R

K

R

R

Q

Q

E

H

E

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

Y

F

T

V

S

S

P

R

A

A

V

V

M

M

A

E

A

A

Q

Q

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y

Y

G

G

C

C

E

E

Y

Y

V

V

D

D

V

I

A

V

E

E

Q

E

L

L

A

A

I

R

D

E

R

R

V

A

K

K

Q

Q

L

L

F

F

G

G

A

A

E

E

A

H

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

Q

M

G

A

V

V

F

Y

F

F

A

T

M

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

S

N

L

L

A

S

H

H

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

V

W

Q

F

Y

N

N

V

F

V

V

S

A

Y

Y

G

G

L

V

N

D

-

P

E

E

A

T

E

H

D

V

I

I

D

D

Y

Y

D

D

A

D

A

V

E

R

K

E

L

K

A

A

Q

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

V

V

A

A

G

A

A

A

S
|
S

A

A

F

Y

A

P

L

R

R

I

I

I

D

D

F

F

E

A

R

K

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

Y

F

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

P

H

Y

A

A

D

H

F

F

V

V

T

T

T
|
T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

V

M

I

I

L

L

M

C

K

Q

A

E

E

Q

F

F

E

A

K

K

Q

Q

I

I

N

D

S

K

A

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

K

G

E

E

A

A

L

L

S

Q

P

D

E

D

F

F

K

K

A

A

Y

Y

Q

A

Q

K

Q

R

V

V

E

D

N

N

A

A

R

K

V

R

L

L

A

A

E

S

T

A

L

L

V

Q

K

N

R

E

G

G

L

F

R

T

I

L

V

V

S

S

G

G

R

G

T

T

E

D

S

N

H

H

V

L

M

L

L

L

V

V

D

D

L

L

R

R

A

P

K

Q

I

L

T

T

G

G

K

K

A

T

A

A

E

E

A

K

A

V

L

L

G

D

A

E

A

V

H

G

I

I

T

T

V

V

N

N

K

K

N

N

A

T

I

I

P

P

N

Y

D

D

P

P

E

E

K

S

P

P

F

F

V

V

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

S

T

P

A

A

A

M

V

T

T

R

R

G

G

F

F

G

G

T

L

K

E

E

E

A

M

E

D

Q

E

V

I

G

A

N

A

L

I

I

I

A

G

D

L

V

V

L

L

D

K

N

N

P

V

E

G

D

S

T

E

A

Q

T

A

I

L

D

E

R

E

V

A

R

R

G

Q

Q

R

V

V

A

A

E

A

L

L

T

T

active site: Y51 (= Y56), E53 (= E58), D197 (= D201), T223 (= T227), K226 (= K230), R232 (= R236)
binding pyridoxal-5'-phosphate: S93 (= S98), G94 (= G99), A95 (≠ S100), H122 (= H127), S172 (= S176), D197 (= D201), A199 (= A203), H200 (= H204), K226 (= K230)
binding serine: S31 (= S36), H122 (= H127), R357 (= R361)

1kkjA Crystal structure of serine hydroxymethyltransferase from b.Stearothermophilus (see paper)
62% identity, 95% coverage: 13:408/415 of query aligns to 8:404/405 of 1kkjA

query
sites
1kkjA

D

D

P

P

E

Q

L

V

W

F

K

A

V

A

I

I

E

E

Q

Q

E

E

N

R

R

K

R

R

Q

Q

E

H

E

A

H

K

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

Y

F

T

V

S

S

P

R

A

A

V

V

M

M

A

E

A

A

Q

Q

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y

Y

G

G

C

C

E

E

Y

Y

V

V

D

D

V

I

A

V

E

E

Q

E

L

L

A

A

I

R

D

E

R

R

V

A

K

K

Q

Q

L

L

F

F

G

G

A

A

E

E

A

H

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

Q

M

G

A

V

V

F

Y

F

F

A

T

M

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

S

N

L

L

A

S

H

H

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

V

W

Q

F

Y

N

N

V

F

V

V

S

A

Y

Y

G

G

L

V

N

D

-

P

E

E

A

T

E

H

D

V

I

I

D

D

Y

Y

D

D

A

D

A

V

E

R

K

E

L

K

A

A

Q

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

V

V

A

A

G

A

A

A

S
|
S

A

A

F

Y

A

P

L

R

R

I

I

I

D

D

F

F

E

A

R

K

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

Y

F

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

P

H

Y

A

A

D

H

F

F

V

V

T

T

T
|
T

T

T

H
|
H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

V

M

I

I

L

L

M

C

K

Q

A

E

E

Q

F

F

E

A

K

K

Q

Q

I

I

N

D

S

K

A

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

K

G

E

E

A

A

L

L

S

Q

P

D

E

D

F

F

K

K

A

A

Y

Y

Q

A

Q

K

Q

R

V

V

E

D

N

N

A

A

R

K

V

R

L

L

A

A

E

S

T

A

L

L

V

Q

K

N

R

E

G

G

L

F

R

T

I

L

V

V

S

S

G

G

R

G

T

T

E

D

S

N

H

H

V

L

M

L

L

L

V

V

D

D

L

L

R

R

A

P

K

Q

I

L

T

T

G

G

K

K

A

T

A

A

E

E

A

K

A

V

L

L

G

D

A

E

A

V

H

G

I

I

T

T

V

V

N

N

K

K

N

N

A

T

I

I

P

P

N

Y

D

D

P

P

E

E

K

S

P

P

F

F

V

V

T

T

S

S

G

G

I

I

R

R

L

I

G

G

S

T

P

A

A

A

M

V

T

T

R

R

G

G

F

F

G

G

T

L

K

E

E

E

A

M

E

D

Q

E

V

I

G

A

N

A

L

I

I

I

A

G

D

L

V

V

L

L

D

K

N

N

P

V

E

G

D

S

T

E

A

Q

T

A

I

L

D

E

R

E

V

A

R

R

G

Q

Q

R

V

V

A

A

E

A

L

L

T

T

active site: Y51 (= Y56), E53 (= E58), D197 (= D201), T223 (= T227), K226 (= K230), R232 (= R236)
binding pyridoxal-5'-phosphate: S93 (= S98), G94 (= G99), A95 (≠ S100), H122 (= H127), S172 (= S176), D197 (= D201), A199 (= A203), H200 (= H204), T223 (= T227), H225 (= H229), K226 (= K230)

2vmyA Crystal structure of f351gbsshmt in complex with gly and fthf (see paper)
62% identity, 95% coverage: 13:408/415 of query aligns to 8:404/405 of 2vmyA

query
sites
2vmyA

D

D

P

P

E

Q

L

V

W

F

K

A

V

A

I

I

E

E

Q

Q

E

E

N

R

R

K

R

R

Q

Q

E

H

E

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

Y

F

T

V

S

S

P

R

A

A

V

V

M

M

A

E

A

A

Q

Q

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y
|
Y

G

G

C

C

E

E

Y

Y

V

V

D

D

V

I

A

V

E

E

Q

E

L

L

A

A

I

R

D

E

R

R

V

A

K

K

Q

Q

L

L

F

F

G

G

A

A

E

E

A

H

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

Q

M

G

A

V

V

F

Y

F

F

A

T

M

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

S

N

L
|
L

A

S

H

H

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

V

W

Q

F

Y

N

N

V

F

V

V

S

A

Y

Y

G

G

L

V

N

D

-

P

E

E

A

T

E

H

D

V

I

I

D

D

Y

Y

D

D

A

D

A

V

E

R

K

E

L

K

A

A

Q

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

V

V

A

A

G

A

A

A

S
|
S

A

A

F

Y

A

P

L

R

R

I

I

I

D

D

F

F

E

A

R

K

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

Y

F

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

P

H

Y

A

A

D

H

F

F

V

V

T

T

T
|
T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

V

M

I

I

L

L

M

C

K

Q

A

E

E

Q

F

F

E

A

K

K

Q

Q

I

I

N

D

S
x
K

A

A

I

I

F
|
F

P

P

G

G

I

I

Q

Q

G

G

G
|
G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

K

G

E

E

A

A

L

L

S

Q

P

D

E

D

F

F

K

K

A

A

Y

Y

Q

A

Q

K

Q

R

V

V

E

D

N

N

A

A

R

K

V

R

L

L

A

A

E

S

T

A

L

L

V

Q

K

N

R

E

G

G

L

F

R

T

I

L

V

V

S

S

G

G

R

G

T

T

E

D

S

N

H

H

V

L

M

L

L

L

V

V

D

D

L

L

R

R

A

P

K

Q

I

L

T

T

G

G

K

K

A

T

A

A

E

E

A

K

A

V

L

L

G

D

A

E

A

V

H

G

I

I

T

T

V

V

N

N

K

K

N
|
N

A

T

I

I

P

P

N

Y

D

D

P

P

E

E

K
x
S

P
|
P

F
x
G

V

V

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

S

T

P

A

A

A

M

V

T

T

R

R

G

G

F

F

G

G

T

L

K

E

E

E

A

M

E

D

Q

E

V

I

G

A

N

A

L

I

I

I

A

G

D

L

V

V

L

L

D

K

N

N

P

V

E

G

D

S

T

E

A

Q

T

A

I

L

D

E

R

E

V

A

R

R

G

Q

Q

R

V

V

A

A

E

A

L

L

T

T

active site: Y51 (= Y56), E53 (= E58), D197 (= D201), T223 (= T227), K226 (= K230), R232 (= R236)
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E53 (= E58), Y60 (= Y65), Y61 (= Y66), L117 (= L122), G121 (= G126), H122 (= H127), L123 (= L128), S172 (= S176), K248 (≠ S252), F251 (= F255), N341 (= N345), S349 (≠ K353), P350 (= P354), G351 (≠ F355), R357 (= R361)
binding glycine: S31 (= S36), Y51 (= Y56), Y61 (= Y66), H200 (= H204), K226 (= K230), R357 (= R361)
binding pyridoxal-5'-phosphate: Y51 (= Y56), S93 (= S98), G94 (= G99), A95 (≠ S100), H122 (= H127), S172 (= S176), D197 (= D201), A199 (= A203), H200 (= H204), T223 (= T227), K226 (= K230), G257 (= G261)

2vmxA Crystal structure of f351gbsshmt in complex with l-allo-thr (see paper)
62% identity, 95% coverage: 13:408/415 of query aligns to 8:404/405 of 2vmxA

query
sites
2vmxA

D

D

P

P

E

Q

L

V

W

F

K

A

V

A

I

I

E

E

Q

Q

E

E

N

R

R

K

R

R

Q

Q

E

H

E

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

Y

F

T

V

S

S

P

R

A

A

V

V

M

M

A

E

A

A

Q

Q

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y

Y

G

G

C

C

E

E

Y

Y

V

V

D

D

V

I

A

V

E

E

Q

E

L

L

A

A

I

R

D

E

R

R

V

A

K

K

Q

Q

L

L

F

F

G

G

A

A

E

E

A

H

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

Q

M

G

A

V

V

F

Y

F

F

A

T

M

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

S

N

L

L

A

S

H

H

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

V

W

Q

F

Y

N

N

V

F

V

V

S

A

Y

Y

G

G

L

V

N

D

-

P

E

E

A

T

E

H

D

V

I

I

D

D

Y

Y

D

D

A

D

A

V

E

R

K

E

L

K

A

A

Q

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

V

V

A

A

G

A

A

A

S
|
S

A

A

F

Y

A

P

L

R

R

I

I

I

D

D

F

F

E

A

R

K

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

Y

F

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

P

H

Y

A

A

D

H

F

F

V

V

T

T

T
|
T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

V

M

I

I

L

L

M

C

K

Q

A

E

E

Q

F

F

E

A

K

K

Q

Q

I

I

N

D

S

K

A

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

K

G

E

E

A

A

L

L

S

Q

P

D

E

D

F

F

K

K

A

A

Y

Y

Q

A

Q

K

Q

R

V

V

E

D

N

N

A

A

R

K

V

R

L

L

A

A

E

S

T

A

L

L

V

Q

K

N

R

E

G

G

L

F

R

T

I

L

V

V

S

S

G

G

R

G

T

T

E

D

S

N

H

H

V

L

M

L

L

L

V

V

D

D

L

L

R

R

A

P

K

Q

I

L

T

T

G

G

K

K

A

T

A

A

E

E

A

K

A

V

L

L

G

D

A

E

A

V

H

G

I

I

T

T

V

V

N

N

K

K

N

N

A

T

I

I

P

P

N

Y

D

D

P

P

E

E

K

S

P

P

F

G

V

V

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

S

T

P

A

A

A

M

V

T

T

R

R

G

G

F

F

G

G

T

L

K

E

E

E

A

M

E

D

Q

E

V

I

G

A

N

A

L

I

I

I

A

G

D

L

V

V

L

L

D

K

N

N

P

V

E

G

D

S

T

E

A

Q

T

A

I

L

D

E

R

E

V

A

R

R

G

Q

Q

R

V

V

A

A

E

A

L

L

T

T

active site: Y51 (= Y56), E53 (= E58), D197 (= D201), T223 (= T227), K226 (= K230), R232 (= R236)
binding allo-threonine: S31 (= S36), H122 (= H127), H200 (= H204), R357 (= R361)
binding pyridoxal-5'-phosphate: S93 (= S98), G94 (= G99), A95 (≠ S100), H122 (= H127), S172 (= S176), D197 (= D201), A199 (= A203), H200 (= H204), T223 (= T227), K226 (= K230)

7x5oB Crystal structure of e. Faecium shmt in complex with me-thf and plp- gly (see paper)
60% identity, 97% coverage: 13:414/415 of query aligns to 7:411/412 of 7x5oB

query
sites
7x5oB

D

D

P

P

E

D

L

L

W

W

K

A

V

A

I

I

E

A

Q

K

E

E

N

E

R

E

R

R

Q

Q

E

E

E

H

H

N

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

Y

F

T

V

S

S

P

E

A

A

V

V

M

M

A

A

Q

Q

G

G

S

S

Q

I

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

H

R

R

Y
|
Y

G

G

C

C

E

E

Y

F

V

V

D

D

V

I

A

V

E

E

Q

N

L

L

A

A

I

I

D

D

R

R

V

A

K

K

Q

E

L

L

F

F

G

G

A

A

E

K

A

F

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

Q

T

G

A

V

A

F

Y

F

L

A

A

M

L

L

V

K

E

P

P

G

G

D

D

T

T

I

I

M

L

G

G

M

M

S

D

L
|
L

A

S

H

A

G
|
G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

K

W

T

F

Y

N

H

V

F

V

V

S

A

Y

Y

G

G

L

V

N

D

-

P

E

T

A

T

E

E

D

V

I

I

D

D

Y

Y

D

N

A

V

E

R

K

I

L

L

A

A

Q

R

E

K

H

H

K

Q

P

P

K

K

L

L

I

I

V

V

A

A

G

G

A

A

S
|
S

A

A

F

Y

A

G

L

R

R

T

I

I

D

D

F

F

E

A

R

K

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

K

F

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

P

H

Y

A

A

D

D

F

I

V

T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R

R

G

G

V

M

I

I

L

L

M

T

K

N

A

D

E

E

-

A

F

L

E

A

K

K

Q

K

I

I

N

N

S

S

A

A

I

V

F

F

P
|
P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

G

G

K

K

A

A

V

V

A

A

F

F

K

K

E

E

A

A

L

L

S

D

P

P

E

A

F

F

K

K

A

E

Y

Y

Q

S

Q

E

Q

Q

V

I

E

A

N

N

A

A

R

K

V

A

L

M

A

V

E

K

T

V

L

F

V

N

K

Q

R

A

-

I

G

G

L

T

R

R

I

V

V

I

S

S

G

G

R

A

T

T

E

D

S

N

H

H

V

L

M

M

L

L

V

I

D

D

L

V

R

R

A

E

K

L

K

G

I

I

T

N

G

G

K

K

A

E

A

A

E

E

A

S

A

I

L

L

G

D

A

S

A

V

H

N

I

I

T

T

V

V

N

N

K

K

N
|
N

A

S

I

I

P

P

N

F

D

E

P

T

E

L

K

S

P
|
P

F

F

V

K

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

S

T

P

P

A

A

M

I

T

T

R

R

G

G

F

F

G

K

T

E

K

E

E

D

A

A

E

V

Q

K

V

V

G

A

N

E

L

L

I

V

A

V

D

K

V

A

L

L

D

Q

N

A

P

K

E

D

D

D

T

N

A

A

T

Q

I

L

D

D

R

E

V

V

R

K

G

T

Q

G

V

V

A

R

E

E

L

L

T

T

Q

E

R

K

F

F

P

P

V

L

Y

H

binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: S30 (= S36), Y50 (= Y56), Y60 (= Y66), S92 (= S98), G93 (= G99), S94 (= S100), H121 (= H127), S171 (= S176), D196 (= D201), A198 (= A203), H199 (= H204), K225 (= K230), R358 (= R361)
binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: E52 (= E58), Y59 (= Y65), L116 (= L122), G119 (= G125), G120 (= G126), H121 (= H127), S171 (= S176), P252 (= P256), N342 (= N345), P351 (= P354)

7x5nA Crystal structure of e. Faecium shmt in complex with (+)-shin-1 and plp-ser (see paper)
60% identity, 97% coverage: 13:413/415 of query aligns to 6:409/409 of 7x5nA

query
sites
7x5nA

D

D

P

P

E

D

L

L

W

W

K

A

V

A

I

I

E

A

Q

K

E

E

N

E

R

E

R

R

Q

Q

E

E

E

H

H

N

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

Y

F

T

V

S

S

P

E

A

A

V

V

M

M

A

A

Q

Q

G

G

S

S

Q

I

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

H

R

R

Y
|
Y

G

G

C

C

E

E

Y

F

V

V

D

D

V

I

A

V

E

E

Q

N

L

L

A

A

I

I

D

D

R

R

V

A

K

K

Q

E

L

L

F

F

G

G

A

A

E

K

A

F

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

Q

T

G

A

V

A

F

Y

F

L

A

A

M

L

L

V

K

E

P

P

G

G

D

D

T

T

I

I

M

L

G

G

M

M

S

D

L
|
L

A

S

H

A

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

K

W

T

F

Y

N

H

V

F

V

V

S

A

Y

Y

G

G

L

V

N

D

-

P

E

T

A

T

E

E

D

V

I

I

D

D

Y

Y

D

N

A

V

E

R

K

I

L

L

A

A

Q

R

E

K

H

H

K

Q

P

P

K

K

L

L

I

I

V

V

A

A

G

G

A

A

S
|
S

A

A

F

Y

A

G

L

R

R

T

I

I

D

D

F

F

E

A

R

K

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

K

F

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

P

H

Y

A

A

D

D

F

I

V

T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R

R

G

G

V

M

I

I

L

L

M

T

K

N

A

D

E

E

-

A

F

L

E

A

K

K

Q

K

I

I

N

N

S

S

A

A

I

V

F

F

P

P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

G

G

K

K

A

A

V

V

A

A

F

F

K

K

E

E

A

A

L

L

S

D

P

P

E

A

F

F

K

K

A

E

Y

Y

Q

S

Q

E

Q

Q

V

I

E

A

N

N

A

A

R

K

V

A

L

M

A

V

E

K

T

V

L

F

V

N

K

Q

R

A

-

I

G

G

L

T

R

R

I

V

V

I

S

S

G

G

R

A

T

T

E

D

S

N

H

H

V

L

M

M

L

L

V

I

D

D

L

V

R

R

A

E

K

L

K

G

I

I

T

N

G

G

K

K

A

E

A

A

E

E

A

S

A

I

L

L

G

D

A

S

A

V

H

N

I

I

T

T

V

V

N

N

K
|
K

N
|
N

A
x
S

I

I

P

P

N

F

D

E

P

T

E

L

K

S

P
|
P

F
|
F

V

K

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

S

T

P

P

A

A

M

I

T

T

R

R

G

G

F

F

G

K

T

E

K

E

E

D

A

A

E

V

Q

K

V

V

G

A

N

E

L

L

I

V

A

V

D

K

V

A

L

L

D

Q

N

A

P

K

E

D

D

D

T

N

A

A

T

Q

I

L

D

D

R

E

V

V

R

K

G

T

Q

G

V

V

A

R

E

E

L

L

T

T

Q

E

R

K

F

F

P

P

V

L

binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E58), Y58 (= Y65), Y59 (= Y66), L115 (= L122), G119 (= G126), H120 (= H127), L121 (= L128), K340 (= K344), N341 (= N345), S342 (≠ A346), P350 (= P354), F351 (= F355), R357 (= R361)
binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S29 (= S36), Y49 (= Y56), E51 (= E58), Y59 (= Y66), S91 (= S98), G92 (= G99), S93 (= S100), H120 (= H127), S170 (= S176), D195 (= D201), A197 (= A203), H198 (= H204), K224 (= K230), R357 (= R361)

7v3dA Complex structure of serine hydroxymethyltransferase from enterococcus faecium and its inhibitor (see paper)
60% identity, 97% coverage: 13:413/415 of query aligns to 6:409/409 of 7v3dA

query
sites
7v3dA

D

D

P

P

E

D

L

L

W

W

K

A

V

A

I

I

E

A

Q

K

E

E

N

E

R

E

R

R

Q

Q

E

E

E

H

H

N

I

L

E

E

L

L

I

I

A

A

S

S

E

E

N

N

Y

F

T

V

S

S

P

E

A

A

V

V

M

M

A

A

Q

Q

G

G

S

S

Q

I

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

H

R

R

Y
|
Y

Y

Y

G

G

C

C

E

E

Y

F

V

V

D

D

V

I

A

V

E

E

Q

N

L

L

A

A

I

I

D

D

R

R

V

A

K

K

Q

E

L

L

F

F

G

G

A

A

E

K

A

F

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

Q

T

G

A

V

A

F

Y

F

L

A

A

M

L

L

V

K

E

P

P

G

G

D

D

T

T

I

I

M

L

G

G

M

M

S

D

L
|
L

A

S

H

A

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

K

W

T

F

Y

N

H

V

F

V

V

S

A

Y

Y

G

G

L

V

N

D

-

P

E

T

A

T

E

E

D

V

I

I

D

D

Y

Y

D

N

A

V

E

R

K

I

L

L

A

A

Q

R

E

K

H

H

K

Q

P

P

K

K

L

L

I

I

V

V

A

A

G

G

A

A

S
|
S

A

A

F

Y

A

G

L

R

R

T

I

I

D

D

F

F

E

A

R

K

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

K

F

L

M

M

V

V

D
|
D

M

M

A
|
A

H

H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

P

H

Y

A

A

D

D

F

I

V

T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R

R

G

G

V

M

I

I

L

L

M

T

K

N

A

D

E

E

-

A

F

L

E

A

K

K

Q

K

I

I

N

N

S

S

A

A

I

V

F

F

P

P

G

G

I

I

Q

Q

G
|
G

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

G

G

K

K

A

A

V

V

A

A

F

F

K

K

E

E

A

A

L

L

S

D

P

P

E

A

F

F

K

K

A

E

Y

Y

Q

S

Q

E

Q

Q

V

I

E

A

N

N

A

A

R

K

V

A

L

M

A

V

E

K

T

V

L

F

V

N

K

Q

R

A

-

I

G

G

L

T

R

R

I

V

V

I

S

S

G

G

R

A

T

T

E

D

S

N

H

H

V

L

M

M

L

L

V

I

D

D

L

V

R

R

A

E

K

L

K

G

I

I

T

N

G

G

K

K

A

E

A

A

E

E

A

S

A

I

L

L

G

D

A

S

A

V

H

N

I

I

T

T

V

V

N

N

K
|
K

N

N

A
x
S

I

I

P

P

N

F

D

E

P

T

E

L

K

S

P
|
P

F
|
F

V

K

T

T

S

S

G

G

I

I

R
|
R

L

I

G

G

S

T

P

P

A

A

M

I

T

T

R

R

G

G

F

F

G

K

T

E

K

E

E

D

A

A

E

V

Q

K

V

V

G

A

N

E

L

L

I

V

A

V

D

K

V

A

L

L

D

Q

N

A

P

K

E

D

D

D

T

N

A

A

T

Q

I

L

D

D

R

E

V

V

R

K

G

T

Q

G

V

V

A

R

E

E

L

L

T

T

Q

E

R

K

F

F

P

P

V

L

binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E58), Y58 (= Y65), L115 (= L122), G119 (= G126), H120 (= H127), L121 (= L128), K340 (= K344), S342 (≠ A346), P350 (= P354), F351 (= F355), R357 (= R361)
binding pyridoxal-5'-phosphate: Y49 (= Y56), S91 (= S98), G92 (= G99), S93 (= S100), H120 (= H127), S170 (= S176), D195 (= D201), A197 (= A203), K224 (= K230), G255 (= G260)

3pgyB Serine hydroxymethyltransferase from staphylococcus aureus, s95p mutant.
59% identity, 98% coverage: 7:414/415 of query aligns to 2:404/404 of 3pgyB

query
sites
3pgyB

S

S

T

Y

I

I

A

T

N

K

V

Q

D

D

P

K

E

V

L

I

W

A

K

E

V

A

I

I

E

E

Q

R

E

E

N

F

R

Q

R

R

Q

Q

E

N

E

S

H

N

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

Y

F

T

V

S

S

P

E

A

A

V

V

M

M

A

E

A

A

Q

Q

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y

Y

G

G

C

C

E

E

Y

F

V

V

D

D

V

V

A

T

E

E

Q

S

L

I

A

A

I

I

D

D

R

R

V

A

K

K

Q

A

L

L

F

F

G

G

A

A

E

E

A

H

A

V

N

N

V

V

Q

Q

P

P

N

H

S

S

G

G

S

P

Q

Q

A

A

N

N

Q

M

G

A

V

V

F

Y

F

L

A

V

M

A

L

L

K

E

P

M

G

G

D

D

T

T

I

V

M

L

G

G

M

M

S

N

L

L

A

S

H

H

G

-

H

-

L

-

T

-

H

-

G

-

S

A

P

P

V

V

N

N

M

F

S

S

G

G

K

K

W

F

F

Y

N

N

V

F

V

V

S

E

Y

Y

G

G

L

V

N

D

-

K

E

D

A

T

E

E

D

R

I

I

D

N

Y

Y

D

D

A

E

A

V

E

R

K

K

L

L

A

A

Q

L

E

E

H

H

K

K

P

P

K

K

L

L

I

I

V

V

A

A

G

G

A

A

S

S

A

A

F

Y

A

S

L

R

R

T

I

I

D

D

F

F

E

K

R

K

L

F

S

K

K

E

I

I

A

A

K

D

S

E

V

V

G

N

A

A

Y

K

F

L

M

M

V

V

D
|
D

M

M

A

A

H

H

Y

I

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

V

P

E

H

Y

A

A

D

D

F

F

V

V

T

T

T
|
T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

V

M

I

I

L

L

M

C

K

K

A

E

E

E

F

Y

E

K

K

K

Q

D

I

I

N

D

S

K

A

T

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

G

A

K

K

A

A

V

V

A

A

F

F

K

G

E

E

A

A

L

L

S

E

P

N

E

N

F

F

K

K

A

T

Y

Y

Q

Q

V

V

E

K

N

N

A

A

R

K

V

V

L

L

A

A

E

E

T

A

L

L

V

I

K

N

R

E

G

G

L

F

R

R

I

I

V

V

S

S

G

G

R

G

T

T

E

D

S

N

H

H

V

L

M

V

L

A

V

V

D

D

L

V

R

K

A

G

K

S

-

I

K

G

I

L

T

T

G

G

K

K

A

E

A

A

E

E

A

E

A

T

L

L

G

D

A
x
S

A

V

H

G

I

I

T

T

V

C

N

N

K

K

N

N

A

T

I

I

P

P

N

F

D

D

P

Q

E

E

K

K

P

P

F

F

V

V

T

T

S

S

G

G

I

I

R

R

L

L

G

G

S

T

P

P

A

A

M

A

T

T

R

R

G

G

F

F

G

D

T

E

K

K

E

A

A

F

E

E

Q

E

V

V

G

A

N

K

L

I

I

I

A

S

D

L

V

A

L

L

D

K

N

N

P

S

E

K

D

D

T
x
E

A

E

T

K

I
x
L

D

Q

R

Q

V

A

R

K

G

E

Q

R

V

V

A

A

E

K

L

L

T

T

Q

A

R

E

F

Y

P

P

V
x
L

Y
|
Y

active site: Y51 (= Y56), E53 (= E58), D190 (= D201), T216 (= T227), K219 (= K230), R225 (= R236)
binding glycine: S327 (≠ A337), E384 (≠ T394), L387 (≠ I397), L403 (≠ V413), Y404 (= Y414)

Q5SI56 Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
61% identity, 94% coverage: 13:401/415 of query aligns to 8:398/407 of Q5SI56

query
sites
Q5SI56

D

D

P

E

E

A

L

L

W

F

K

E

V

L

I

I

E

A

Q

L

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N

E

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K

R

R

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Q

E

R

E

E

H

G

I

L

E

E

L

L

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I

A

A

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S

E

E

N

N

Y

F

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S

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A

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V

M

R

A

E

A

A

Q

V

G

G

S

S

Q

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E

E

G

G

Y

Y

P

P

G

G

K

A

R

R

Y

Y

G

G

C

C

E

E

Y

V

V

I

D

D

V

R

A

V

E

E

Q

S

L

L

A

A

I

I

D

E

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R

V

A

K

K

Q

A

L

L

F

F

G

G

A

A

E

A

A

W

A

A

N

N

V

V

Q

Q

P

P

N

H

S

S

G
|
G

S
|
S

Q

Q

A

A

N

N

Q

M

G

A

V

V

F

Y

F

M

A

A

M

L

L

M

K

E

P

P

G

G

D

D

T

T

I

L

M

M

G

G

M

M

S

D

L

L

A

A

H

A

G

G

H

H

L

L

T

T

H

H

G

G

S

S

P

R

V

V

N

N

M

F

S

S

G

G

K

K

W

L

F

Y

N

K

V

V

S

S

Y

Y

G

G

L

V

N

R

-

P

E

D

A

T

E

E

D

L

I

I

D

D

Y

L

D

E

A

E

A

V

E

R

K

R

L

L

A

A

Q

L

E

E

H

H

K

R

P

P

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K

L

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I

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A

G

G

A

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S
|
S

A

A

F

Y

A

P

L

R

R

F

I

W

D

D

F

F

E

K

R

A

L

F

S

R

K

E

I

I

A

A

K

D

S

E

V

V

G

G

A

A

Y

Y

F

L

M

V

V

V

D

D

M

M

A

A

H
|
H

Y

F

A

A

G

G

L

L

I

V

A

A

G

G

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L

Y

H

P

P

N

N

P

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L

P

P

H

Y

A

A

D

H

F

V

V

V

T

T

T

S

T

T

H
|
H

K
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K

S

T

L

L

R

R

G

G

P

P

R

R

G

G

V

L

I

I

L

L

M

S

K

N

-

D

A

P

E

E

F

L

E

G

K

K

Q

R

I

I

N

D

S

K

A

L

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I

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F

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P

G

G

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I

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G

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G
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G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

G

G

K

K

A

A

V

V

A

A

F

F

K

F

E

E

A

A

L

L

S

Q

P

P

E

E

F

F

K

K

A

E

Y

Y

Q

S

Q

R

Q

L

V

V

E

E

N

N

A

A

R

K

V

R

L

L

A

A

E

E

T

E

L

L

V

A

K

R

R

R

G

G

L

Y

R

R

I

I

V

V

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T

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G

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G

T

T

E

D

S

N

H

H

V

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M

F

L

L

V

V

D

D

L

L

R

R

A

P

K

K

K

G

I

L

T

T

G

G

K

K

A

E

A

A

E

E

A

E

A

R

L

L

G

D

A

A

A

V

H

G

I

I

T

T

V

V

N

N

K

K

N

N

A

A

I

I

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N

F

D

D

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E

K

K

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R

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V

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S

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R

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A

A

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I

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G

T

T

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K

E

E

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L

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V

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A

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E

L

L

I

I

A

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D

R

V

A

L

L

D

L

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E

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D

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A

A

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L

I

R

D

E

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E

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V

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R

Y51 (= Y56) binding
GS 94:95 (= GS 99:100) binding
S172 (= S176) binding
H200 (= H204) binding
H225 (= H229) binding
K226 (= K230) modified: N6-(pyridoxal phosphate)lysine
G258 (= G261) binding

8suiB Joint x-ray/neutron structure of thermus thermophilus serine hydroxymethyltransferase (tthshmt) in internal aldimine state with l- ser bound in a pre-michalis complex (see paper)
61% identity, 94% coverage: 13:401/415 of query aligns to 3:393/402 of 8suiB

query
sites
8suiB

D

D

P

E

E

A

L

L

W

F

K

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L

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A

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R

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Q

E

R

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E

H

G

I

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E

E

L

L

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I

A

A

S

S

E

E

N

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Y

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A

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V

M

R

A

E

A

A

Q

V

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G

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S

Q

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L

T

T

N

N

K

K

Y

Y

A

A

E

E

G

G

Y

Y

P

P

G

G

K

A

R

R

Y
|
Y

G

G

C

C

E

E

Y

V

V

I

D

D

V

R

A

V

E

E

Q

S

L

L

A

A

I

I

D

E

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R

V

A

K

K

Q

A

L

L

F

F

G

G

A

A

E

A

A

W

A

A

N

N

V

V

Q

Q

P

P

N

H

S

S

G

G

S

S

Q

Q

A

A

N

N

Q

M

G

A

V

V

F

Y

F

M

A

A

M

L

L

M

K

E

P

P

G

G

D

D

T

T

I

L

M

M

G

G

M

M

S

D

L

L

A

A

H

A

G

G

H

H

L

L

T

T

H

H

G

G

S

S

P

R

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V

N

N

M

F

S

S

G

G

K

K

W

L

F

Y

N

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S

S

Y

Y

G

G

L

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N

R

-

P

E

D

A

T

E

E

D

L

I

I

D

D

Y

L

D

E

A

E

A

V

E

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K

R

L

L

A

A

Q

L

E

E

H

H

K

R

P

P

K

K

L

V

I

I

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V

A

A

G

G

A

A

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S

A

A

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Y

A

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L

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R

F

I

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D

D

F

F

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K

R

A

L

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S

R

K

E

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I

A

A

K

D

S

E

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V

G

G

A

A

Y

Y

F

L

M

V

V

V

D

D

M

M

A

A

H

H

Y

F

A

A

G

G

L

L

I

V

A

A

G

G

V

L

Y

H

P

P

N

N

P

P

V

L

P

P

H

Y

A

A

D

H

F

V

V

V

T

T

T

S

T

T

H

H

K

K

S

T

L

L

R

R

G

G

P

P

R

R

G

G

V

L

I

I

L

L

M

S

K

N

-

D

A

P

E

E

F

L

E

G

K

K

Q

R

I

I

N

D

S

K

A

L

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I

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F

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P

G

G

I

I

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Q

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G

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P

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E

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H

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V

I

I

A

A

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G

K

K

A

A

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V

A

A

F

F

K

F

E

E

A

A

L

L

S

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P

E

E

F

F

K

K

A

E

Y

Y

Q

S

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R

Q

L

V

V

E

E

N

N

A

A

R

K

V

R

L

L

A

A

E

E

T

E

L

L

V

A

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R

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G

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Y

R

R

I

I

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G

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S

N

H

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L

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V

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D

L

L

R

R

A

P

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G

I

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T

T

G

G

K

K

A

E

A

A

E

E

A

E

A

R

L

L

G

D

A

A

A

V

H

G

I

I

T

T

V

V

N

N

K

K

N

N

A

A

I

I

P

P

N

F

D

D

P

P

E

K

K

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P

P

F

R

V

V

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T

S

S

G

G

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I

R

R

L

I

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G

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P

P

A

A

M

I

T

T

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R

G

G

F

F

G

T

T

P

K

E

E

E

A

M

E

P

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L

V

V

G

A

N

E

L

L

I

I

A

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D

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V

A

L

L

D

L

N

E

P

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E

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T

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A

A

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D

E

R

E

V

V

R

R

binding serine: Y55 (= Y65), Y56 (= Y66)

Query Sequence

>H281DRAFT_04726 FitnessBrowser__Burk376:H281DRAFT_04726
MFDRAQSTIANVDPELWKVIEQENRRQEEHIELIASENYTSPAVMAAQGSQLTNKYAEGY
PGKRYYGGCEYVDVAEQLAIDRVKQLFGAEAANVQPNSGSQANQGVFFAMLKPGDTIMGM
SLAHGGHLTHGSPVNMSGKWFNVVSYGLNEAEDIDYDAAEKLAQEHKPKLIVAGASAFAL
RIDFERLSKIAKSVGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVI
LMKAEFEKQINSAIFPGIQGGPLMHVIAGKAVAFKEALSPEFKAYQQQVVENARVLAETL
VKRGLRIVSGRTESHVMLVDLRAKKITGKAAEAALGAAHITVNKNAIPNDPEKPFVTSGI
RLGSPAMTTRGFGTKEAEQVGNLIADVLDNPEDTATIDRVRGQVAELTQRFPVYG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory