SitesBLAST

C26 (≠ K39) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F40) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ L58) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C67) mutation to T: Loss of ATPase activity and transport.
L60 (= L73) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I89) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V148) mutation to M: Loss of ATPase activity and transport.
D172 (= D185) mutation to N: Loss of ATPase activity and transport.
C276 (= C288) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E311) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
43% identity, 81% coverage: 31:346/388 of query aligns to 7:332/375 of 2d62A

query
sites
2d62A

V

V

Q

K

I

L

V

I

D

N

V

I

V

W

K

K

K

R

F

F

G

G

E

D

T

V

V

T

A

A

V

V

K

K

Q

D

V

L

N

S

L

L

S

E

V

I

K

K

K

D

G

G

E

E

L

F

F

L

A

V

L

L

G

G

S

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

E

V

P

T

T

S

R

G

G

K

Q

I

I

L

Y

I

I

D

E

G

D

E

N

D

L

L

V

A

A

Q

D

-

P

-

E

-

K

-

G

-

V

-

F

L

V

P

P

Y

K

R

E

R

R

P

D

V

V

N

A

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

E

Y

S

D

N

N

V

I

A

A

F

F

G

P

L

L

K

K

Q

L

E

R

G

K

V

V

P

P

K

K

A

Q

E

E

L

I

K

D

D

K

R

R

V

V

Q

R

T

E

A

V

L

A

E

E

L

M

V

L

Q

G

M

L

G

T

R

E

F

L

A

L

N

N

R

R

K

K

P

P

H

R

Q

E

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

L

A

G

R

R

S

A

L

I

V

I

K

R

R

R

P

P

K

K

L

V

L

F

L

L

L

M

D

D

E

E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

K

I

L

R

R

Q

V

R

K

T

M

Q

R

I

A

E

E

L

L

V

K

N

K

I

L

L

Q

D

R

T

Q

V

L

G

G

V

V

T

T

C

T

I

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

M

A

G

G

D

R

R

L

I

A

A

V

V

M

M

S

N

E

K

G

G

E

E

I

L

V

Q

Q

Q

L

V

G

G

T

T

P

P

H

D

E

E

V

V

Y

Y

E

Y

Y

K

P

P

N

V

S

N

R

T

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

T

P

N

P

L

M

-

N

F

F

E

L

G

D

H

A

T

T

V

I

E

T

D

D

E

D

P

G

D

-

H

-

V

-

F

F

I

L

E

D

T

F

P

G

D

E

L

F

T

K

C

L

R

K

L

L

Y

L

V

Q

N

D

H

Q

G

F

I

E

T

V

-

L

-

E

G

N

P

M

L

V

G

G

M

K

P

E

V

V

T

I

I

F

S

G

V

I

R

R

P

P

E

E

R

D

I

V

-

H

-

D

-

A

A

S

L

F

T

T

R

H

-

I

K

D

P

V

P

P

E

E

G

-

A

-

Y

E

N

N

W

T

G

V

K

K

G

A

V

T

V

V

T

D

N

I

I

I

A

E

Y

N

M

L

G

G

Y

E

S

K

L

I

Y

V

H

H

V

L

K

R

binding pyrophosphate 2-: G42 (= G66), C43 (= C67), G44 (= G68), K45 (= K69), T46 (≠ S70), T47 (= T71)

1g291 Malk (see paper)
42% identity, 85% coverage: 31:359/388 of query aligns to 4:341/372 of 1g291

query
sites
1g291

V

V

Q

R

I

L

V

V

D

D

V

V

V

W

K

K

K

V

F

F

G

G

E

E

T

V

V

T

A

A

V

V

K

R

Q

E

V

M

N

S

L

L

S

E

V

V

K

K

K

D

G

G

E

E

L

F

F

M

A

I

L

L

G

G

S

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

E

V

P

T

S

S

R

G

G

K

Q

I

I

L

Y

I

I

-

G

-

D

-

K

-

L

-

V

-

A

D
|
D

G

P

E
|
E

D
x
K

L

G

A

I

Q

F

L

V

P

P

Y
x
K

R
x
D

R

R

P

D

V

I

N

A

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

E

Y

S

D

N

N

V

I

A

A

F

F

G

P

L

L

K

K

Q

L

E

R

G

K

V

V

P

P

K

R

A

Q

E

E

L

I

K

D

D

Q

R

R

V

V

Q

R

T

E

A

V

L

A

E

E

L

L

V

L

Q

G

M

L

G

T

R

E

F

L

A

L

N

N

R

R

K

K

P

P

H

R

Q

E

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

L

A

G

R

R

S

A

L

I

V

V

K

R

R

K

P

P

K

Q

L

V

L

F

L

L

L

M

D

D

E

E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

K

I

L

R

R

Q

V

R

R

T

M

Q

R

I

A

E

E

L

L

V

K

N

K

I

L

L

Q

D

R

T

Q

V

L

G

G

V

V

T

T

C

T

I

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

M

A

G

G

D

R

R

L

I

A

A

V

V

M

M

S

N

E

R

G

G

E

V

I

L

V

Q

Q

Q

L

V

G

G

T

S

P

P

H

D

E

E

V

V

Y

Y

E

D

Y

K

P

P

N

A

S

N

R

T

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

T

M

N

N

L

F

F

L

E

D

G

A

H

I

T

V

V

T

E

E

D

D

E

G

P

-

D

-

H

-

V

-

F

F

I

V

E

D

T

F

P

G

D

E

L

F

T

R

C

L

R

K

L

L

Y

L

V

P

N

D

H

Q

-

F

-

E

-

V

-

L

G

G

I

E

T

L

G

G

P

Y

L

V

G

G

M

R

P

E

V

V

T

I

I

F

S

G

V

I

R

R

P

P

E
|
E

R
x
D

I

L

-

Y

-

D

-

A

-

M

-

F

A

A

L

Q

T

V

R

R

K

V

P

P

P

G

E

E

G

-

A

-

Y

-

N

N

W

L

G

V

K

R

G

A

V

V

T

E

N

I

I

V

A

E

Y

N

M

L

G

G

G

S

Y

E

S

R

L

I

Y

V

H

R

V

L

K

R

L

V

D

-

S

G

G

G

K

V

T

T

V

F

I

V

A

G

N

S

V

F

T

R

S

S

binding magnesium ion: D69 (= D90), E71 (= E92), K72 (≠ D93), K79 (≠ Y100), D80 (≠ R101), E292 (= E311), D293 (≠ R312)
binding pyrophosphate 2-: S38 (= S65), G39 (= G66), C40 (= C67), G41 (= G68), K42 (= K69), T43 (≠ S70), T44 (= T71)

Sites not aligning to the query:

binding magnesium ion: 359

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
41% identity, 82% coverage: 31:349/388 of query aligns to 3:321/371 of 3puyA

query
sites
3puyA

V

V

Q

Q

I

L

V

Q

D

N

V

V

V

T

K

K

K

A

F
x
W

G

G

E

E

T

V

V

V

A

V

V

S

K

K

Q

D

V

I

N

N

L

L

S

D

V

I

K

H

K

E

G

G

E

E

L

F

F

V

A

V

L

F

L

V

G

G

S

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

T

V

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

D

G

G

E

E

K

D

R

L

M

A

N

Q

D

L

T

P

P

Y

A

R

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

S

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

E

A

G

G

V

A

P

K

K

K

A

E

E

V

L

I

K

N

D

Q

R

R

V

V

Q

N

T

Q

A

V

L

A

E

E

L

V

V

L

Q

Q

M

L

G

A

R

H

F

L

A

L

N

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E
|
E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

A

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

I

E

E

L

I

V

S

N

R

I

L

L

H

D

K

T

R

V

L

G

G

V

R

T

T

C

M

I

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

L

I

A

V

V

V

M

L

S

D

E

A

G

G

E

R

I

V

V

A

Q

Q

L

V

G

G

T

K

P

P

H

L

E

E

V

L

Y

Y

E

H

Y

Y

P

P

N

A

S

D

R

R

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

K

T

M

N

N

L

F

F

L

E

P

G

V

H

K

T

V

V

T

E

A

D

T

E

A

P

I

D

D

H

Q

V

V

F

Q

I

V

E

E

T

L

P

P

D

-

L

M

T

P

C

N

R

R

L

Q

Y

Q

V

V

N

W

H

L

G

P

I

V

T

E

G

S

-

R

-

D

-

V

P

Q

L

V

G

G

M

A

P

N

V

M

T

S

I

L

S

G

V

I

R

R

P

P

E

E

R

H

I

L

A

L

L

-

T

-

R

-

K

-

P

P

P

S

E

D

G

I

A

A

Y

D

N

V

W

I

G

L

K

E

G

G

V

E

V

V

T

Q

N

V

I

V

A

E

Y

Q

M

L

G

G

G

N

Y

E

S

T

L

Q

Y

I

H

H

V

I

K

Q

L

I

D

P

S

S

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F40), S37 (= S65), G38 (= G66), C39 (= C67), G40 (= G68), K41 (= K69), S42 (= S70), T43 (= T71), Q81 (= Q109), R128 (= R156), A132 (≠ Q160), S134 (= S162), G136 (= G164), Q137 (= Q165), E158 (= E186), H191 (= H219)
binding magnesium ion: S42 (= S70), Q81 (= Q109)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
41% identity, 82% coverage: 31:349/388 of query aligns to 3:321/371 of 3puxA

query
sites
3puxA

V

V

Q

Q

I

L

V

Q

D

N

V

V

V

T

K

K

K

A

F
x
W

G

G

E

E

T

V

V

V

A

V

V

S

K

K

Q

D

V

I

N

N

L

L

S

D

V

I

K

H

K

E

G

G

E

E

L

F

F

V

A

V

L

F

L

V

G

G

S

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

T

V

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

D

G

G

E

E

K

D

R

L

M

A

N

Q

D

L

T

P

P

Y

A

R

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

S

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

E

A

G

G

V

A

P

K

K

K

A

E

E

V

L

I

K

N

D

Q

R

R

V

V

Q

N

T

Q

A

V

L

A

E

E

L

V

V

L

Q

Q

M

L

G

A

R

H

F

L

A

L

N

D

R
|
R

K

K

P

P

H

K

Q

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E

E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

A

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

I

E

E

L

I

V

S

N

R

I

L

L

H

D

K

T

R

V

L

G

G

V

R

T

T

C

M

I

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

L

I

A

V

V

V

M

L

S

D

E

A

G

G

E

R

I

V

V

A

Q

Q

L

V

G

G

T

K

P

P

H

L

E

E

V

L

Y

Y

E

H

Y

Y

P

P

N

A

S

D

R

R

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

K

T

M

N

N

L

F

F

L

E

P

G

V

H

K

T

V

V

T

E

A

D

T

E

A

P

I

D

D

H

Q

V

V

F

Q

I

V

E

E

T

L

P

P

D

-

L

M

T

P

C

N

R

R

L

Q

Y

Q

V

V

N

W

H

L

G

P

I

V

T

E

G

S

-

R

-

D

-

V

P

Q

L

V

G

G

M

A

P

N

V

M

T

S

I

L

S

G

V

I

R

R

P

P

E

E

R

H

I

L

A

L

L

-

T

-

R

-

K

-

P

P

P

S

E

D

G

I

A

A

Y

D

N

V

W

I

G

L

K

E

G

G

V

E

V

V

T

Q

N

V

I

V

A

E

Y

Q

M

L

G

G

G

N

Y

E

S

T

L

Q

Y

I

H

H

V

I

K

Q

L

I

D

P

S

S

binding adenosine-5'-diphosphate: W12 (≠ F40), G38 (= G66), C39 (= C67), G40 (= G68), K41 (= K69), S42 (= S70), T43 (= T71), R128 (= R156), S134 (= S162), Q137 (= Q165)
binding beryllium trifluoride ion: S37 (= S65), G38 (= G66), K41 (= K69), Q81 (= Q109), S134 (= S162), G136 (= G164), H191 (= H219)
binding magnesium ion: S42 (= S70), Q81 (= Q109)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
41% identity, 82% coverage: 31:349/388 of query aligns to 3:321/371 of 3puwA

query
sites
3puwA

V

V

Q

Q

I

L

V

Q

D

N

V

V

V

T

K

K

K

A

F
x
W

G

G

E

E

T

V

V

V

A
x
V

V

S

K

K

Q

D

V

I

N

N

L

L

S

D

V

I

K

H

K

E

G

G

E

E

L

F

F

V

A

V

L

F

L

V

G

G

S

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

T

V

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

D

G

G

E

E

K

D

R

L

M

A

N

Q

D

L

T

P

P

Y

A

R

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

S

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

E

A

G

G

V

A

P

K

K

K

A

E

E

V

L

I

K

N

D

Q

R

R

V

V

Q

N

T

Q

A

V

L

A

E

E

L

V

V

L

Q

Q

M

L

G

A

R

H

F

L

A

L

N

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E
|
E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

A

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

I

E

E

L

I

V

S

N

R

I

L

L

H

D

K

T

R

V

L

G

G

V

R

T

T

C

M

I

I

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

L

I

A

V

V

V

M

L

S

D

E

A

G

G

E

R

I

V

V

A

Q

Q

L

V

G

G

T

K

P

P

H

L

E

E

V

L

Y

Y

E

H

Y

Y

P

P

N

A

S

D

R

R

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

K

T

M

N

N

L

F

F

L

E

P

G

V

H

K

T

V

V

T

E

A

D

T

E

A

P

I

D

D

H

Q

V

V

F

Q

I

V

E

E

T

L

P

P

D

-

L

M

T

P

C

N

R

R

L

Q

Y

Q

V

V

N

W

H

L

G

P

I

V

T

E

G

S

-

R

-

D

-

V

P

Q

L

V

G

G

M

A

P

N

V

M

T

S

I

L

S

G

V

I

R

R

P

P

E

E

R

H

I

L

A

L

L

-

T

-

R

-

K

-

P

P

P

S

E

D

G

I

A

A

Y

D

N

V

W

I

G

L

K

E

G

G

V

E

V

V

T

Q

N

V

I

V

A

E

Y

Q

M

L

G

G

G

N

Y

E

S

T

L

Q

Y

I

H

H

V

I

K

Q

L

I

D

P

S

S

binding adenosine-5'-diphosphate: W12 (≠ F40), V17 (≠ A45), G38 (= G66), C39 (= C67), G40 (= G68), K41 (= K69), S42 (= S70), T43 (= T71), R128 (= R156), A132 (≠ Q160), S134 (= S162), Q137 (= Q165)
binding tetrafluoroaluminate ion: S37 (= S65), G38 (= G66), K41 (= K69), Q81 (= Q109), S134 (= S162), G135 (= G163), G136 (= G164), E158 (= E186), H191 (= H219)
binding magnesium ion: S42 (= S70), Q81 (= Q109)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
41% identity, 82% coverage: 31:349/388 of query aligns to 3:321/371 of 3puvA

query
sites
3puvA

V

V

Q

Q

I

L

V

Q

D

N

V

V

V

T

K

K

K

A

F
x
W

G

G

E

E

T

V

V

V

A
x
V

V

S

K

K

Q

D

V

I

N

N

L

L

S

D

V

I

K

H

K

E

G

G

E

E

L

F

F

V

A

V

L

F

L

V

G

G

S

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

T

V

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

D

G

G

E

E

K

D

R

L

M

A

N

Q

D

L

T

P

P

Y

A

R

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

S

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

E

A

G

G

V

A

P

K

K

K

A

E

E

V

L

I

K

N

D

Q

R

R

V

V

Q

N

T

Q

A

V

L

A

E

E

L

V

V

L

Q

Q

M

L

G

A

R

H

F

L

A

L

N

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G

G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E

E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

A

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

I

E

E

L

I

V

S

N

R

I

L

L

H

D

K

T

R

V

L

G

G

V

R

T

T

C

M

I

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

L

I

A

V

V

V

M

L

S

D

E

A

G

G

E

R

I

V

V

A

Q

Q

L

V

G

G

T

K

P

P

H

L

E

E

V

L

Y

Y

E

H

Y

Y

P

P

N

A

S

D

R

R

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

K

T

M

N

N

L

F

F

L

E

P

G

V

H

K

T

V

V

T

E

A

D

T

E

A

P

I

D

D

H

Q

V

V

F

Q

I

V

E

E

T

L

P

P

D

-

L

M

T

P

C

N

R

R

L

Q

Y

Q

V

V

N

W

H

L

G

P

I

V

T

E

G

S

-

R

-

D

-

V

P

Q

L

V

G

G

M

A

P

N

V

M

T

S

I

L

S

G

V

I

R

R

P

P

E

E

R

H

I

L

A

L

L

-

T

-

R

-

K

-

P

P

P

S

E

D

G

I

A

A

Y

D

N

V

W

I

G

L

K

E

G

G

V

E

V

V

T

Q

N

V

I

V

A

E

Y

Q

M

L

G

G

G

N

Y

E

S

T

L

Q

Y

I

H

H

V

I

K

Q

L

I

D

P

S

S

binding adenosine-5'-diphosphate: W12 (≠ F40), V17 (≠ A45), G38 (= G66), C39 (= C67), G40 (= G68), K41 (= K69), S42 (= S70), T43 (= T71), R128 (= R156), A132 (≠ Q160), S134 (= S162), Q137 (= Q165)
binding magnesium ion: S42 (= S70), Q81 (= Q109)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
41% identity, 82% coverage: 31:349/388 of query aligns to 3:321/374 of 2awnB

query
sites
2awnB

V

V

Q

Q

I

L

V

Q

D

N

V

V

V

T

K

K

K

A

F
x
W

G

G

E

E

T

V

V

V

A
x
V

V

S

K

K

Q

D

V

I

N

N

L

L

S

D

V

I

K

H

K

E

G

G

E

E

L

F

F

V

A

V

L

F

L

V

G

G

S

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

T

V

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

D

G

G

E

E

K

D

R

L

M

A

N

Q

D

L

T

P

P

Y

A

R

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

S

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

E

A

G

G

V

A

P

K

K

K

A

E

E

V

L

I

K

N

D

Q

R

R

V

V

Q

N

T

Q

A

V

L

A

E

E

L

V

V

L

Q

Q

M

L

G

A

R

H

F

L

A

L

N

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E

E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

A

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

I

E

E

L

I

V

S

N

R

I

L

L

H

D

K

T

R

V

L

G

G

V

R

T

T

C

M

I

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

L

I

A

V

V

V

M

L

S

D

E

A

G

G

E

R

I

V

V

A

Q

Q

L

V

G

G

T

K

P

P

H

L

E

E

V

L

Y

Y

E

H

Y

Y

P

P

N

A

S

D

R

R

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

K

T

M

N

N

L

F

F

L

E

P

G

V

H

K

T

V

V

T

E

A

D

T

E

A

P

I

D

D

H

Q

V

V

F

Q

I

V

E

E

T

L

P

P

D

-

L

M

T

P

C

N

R

R

L

Q

Y

Q

V

V

N

W

H

L

G

P

I

V

T

E

G

S

-

R

-

D

-

V

P

Q

L

V

G

G

M

A

P

N

V

M

T

S

I

L

S

G

V

I

R

R

P

P

E

E

R

H

I

L

A

L

L

-

T

-

R

-

K

-

P

P

P

S

E

D

G

I

A

A

Y

D

N

V

W

I

G

L

K

E

G

G

V

E

V

V

T

Q

N

V

I

V

A

E

Y

Q

M

L

G

G

G

N

Y

E

S

T

L

Q

Y

I

H

H

V

I

K

Q

L

I

D

P

S

S

binding adenosine-5'-diphosphate: W12 (≠ F40), V17 (≠ A45), S37 (= S65), G38 (= G66), G40 (= G68), K41 (= K69), S42 (= S70), T43 (= T71)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
41% identity, 82% coverage: 31:349/388 of query aligns to 4:322/371 of P68187

query
sites
P68187

V

V

Q

Q

I

L

V

Q

D

N

V

V

V

T

K

K

K

A

F

W

G

G

E

E

T

V

V

V

A

V

V

S

K

K

Q

D

V

I

N

N

L

L

S

D

V

I

K

H

K

E

G

G

E

E

L

F

F

V

A

V

L

F

L

V

G

G

S

P

S

S

G

G

C

C

G

G

K

K

S

S

T

T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

T

V

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

D

G

G

E

E

K

D

R

L

M

A

N

Q

D

L

T

P

P

Y

A

R

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

S

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

E

A

G

G

V

A

P
x
K

K

K

A

E

E

V

L

I

K

N

D

Q

R

R

V
|
V

Q

N

T

Q

A
x
V

L

A

E
|
E

L

V

V

L

Q

Q

M

L

G
x
A

R

H

F

L

A

L

N

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D
|
D

E

E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

A

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

I

E

E

L

I

V

S

N

R

I

L

L

H

D

K

T

R

V

L

G

G

V

R

T

T

C

M

I

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

L

I

A

V

V

V

M

L

S

D

E

A

G

G

E

R

I

V

V

A

Q

Q

L

V

G

G

T

K

P

P

H

L

E

E

V

L

Y

Y

E

H

Y

Y

P

P

N

A

S

D

R
|
R

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

K

T

M

N

N

L
x
F

F

L

E

P

G

V

H

K

T

V

V

T

E

A

D

T

E

A

P

I

D

D

H

Q

V

V

F

Q

I

V

E

E

T

L

P

P

D

-

L

M

T

P

C

N

R

R

L

Q

Y

Q

V

V

N
x
W

H

L

G

P

I

V

T

E

G

S

-

R

-

D

-

V

P

Q

L

V

G
|
G

M

A

P

N

V

M

T
x
S

I

L

S
x
G

V

I

R

R

P

P

E

E

R

H

I

L

A

L

L

-

T

-

R

-

K

-

P

P

P

S

E

D

G

I

A

A

Y

D

N

V

W

I

G

L

K

E

G
|
G

V

E

V

V

T

Q

N

V

I

V

A
x
E

Y

Q

M

L

G

G

G

N

Y

E

S

T

L

Q

Y

I

H

H

V

I

K

Q

L

I

D

P

S
|
S

A85 (= A112) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ P133) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V141) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A144) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E146) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ G151) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G164) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D185) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (= R255) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (≠ L266) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
W267 (≠ N293) mutation to G: Normal maltose transport but constitutive mal gene expression.
G278 (= G301) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
S282 (≠ T305) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G284 (≠ S307) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G302 (= G329) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
E308 (≠ A335) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
S322 (= S349) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
41% identity, 82% coverage: 31:349/388 of query aligns to 1:319/367 of 1q12A

query
sites
1q12A

V

V

Q

Q

I

L

V

Q

D

N

V

V

V

T

K

K

K

A

F
x
W

G

G

E

E

T

V

V

V

A

V

V

S

K

K

Q

D

V

I

N

N

L

L

S

D

V

I

K

H

K

E

G

G

E

E

L

F

F

V

A

V

L

F

L

V

G

G

S

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

T

V

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

D

G

G

E

E

K

D

R

L

M

A

N

Q

D

L

T

P

P

Y

A

R

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

S

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

E

A

G

G

V

A

P

K

K

K

A

E

E

V

L

I

K

N

D

Q

R

R

V

V

Q

N

T

Q

A

V

L

A

E

E

L

V

V

L

Q

Q

M

L

G

A

R

H

F

L

A

L

N

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E

E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

A

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

I

E

E

L

I

V

S

N

R

I

L

L

H

D

K

T

R

V

L

G

G

V

R

T

T

C

M

I

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

L

I

A

V

V

V

M

L

S

D

E

A

G

G

E

R

I

V

V

A

Q

Q

L

V

G

G

T

K

P

P

H

L

E

E

V

L

Y

Y

E

H

Y

Y

P

P

N

A

S

D

R

R

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

K

T

M

N

N

L

F

F

L

E

P

G

V

H

K

T

V

V

T

E

A

D

T

E

A

P

I

D

D

H

Q

V

V

F

Q

I

V

E

E

T

L

P

P

D

-

L

M

T

P

C

N

R

R

L

Q

Y

Q

V

V

N

W

H

L

G

P

I

V

T

E

G

S

-

R

-

D

-

V

P

Q

L

V

G

G

M

A

P

N

V

M

T

S

I

L

S

G

V

I

R

R

P

P

E

E

R

H

I

L

A

L

L

-

T

-

R

-

K

-

P

P

P

S

E

D

G

I

A

A

Y

D

N

V

W

I

G

L

K

E

G

G

V

E

V

V

T

Q

N

V

I

V

A

E

Y

Q

M

L

G

G

G

N

Y

E

S

T

L

Q

Y

I

H

H

V

I

K

Q

L

I

D

P

S

S

binding adenosine-5'-triphosphate: W10 (≠ F40), S35 (= S65), G36 (= G66), C37 (= C67), G38 (= G68), K39 (= K69), S40 (= S70), T41 (= T71), R126 (= R156), A130 (≠ Q160), S132 (= S162), G134 (= G164), Q135 (= Q165)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 82% coverage: 31:347/388 of query aligns to 4:318/369 of P19566

query
sites
P19566

V

V

Q

Q

I

L

V

R

D

N

V

V

V

T

K

K

K

A

F

W

G

G

E

D

T

V

V

V

A

V

V

S

K

K

Q

D

V

I

N

N

L

L

S

D

V

I

K

H

K

D

G

G

E

E

L

F

F

V

A

V

L

F

L

V

G

G

S

P

S

S

G

G

C

C

G

G

K

K

S

S

T

T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

T

V

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

D

G

G

E

E

T

D

R

L

M

A

N

Q

D

L

I

P

P

Y

A

R

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

T

S

V

V

E

A

S

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

E

A

G

G

V

A

P

K

K

K

A

E

E

V

L

M

K

N

D

Q

R

R

V

V

Q

N

T

Q

A

V

L

A

E

E

L

V

V

L

Q

Q

M

L

G

A

R

H

F

L

A

L

N

E

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

R

L

V

L

F

L

L

D

D

E

E

P
|
P

M

L

S

S

A

N

L

L

D
|
D

K

A

Q

A

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

I

E

E

L

I

V

S

N

R

I

L

L

H

D

K

T

R

V

L

G

G

V

R

T

T

C

M

I

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

A

A

G

D

R

K

L

I

A

V

V

V

M

L

S

D

E

A

G

G

E

R

I

V

V

A

Q

Q

L

V

G

G

T

K

P

P

H

L

E

E

V

L

Y

Y

E

H

Y

Y

P

P

N

A

S

D

R

R

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

K

T

M

N

N

L

F

F

L

E

P

G

V

H

K

T

V

V

T

E

A

D

T

E

A

P

I

D

E

H

Q

V

V

F

Q

I

V

E

E

T

L

P

P

D

N

L

R

T

Q

C

-

R

Q

L

I

Y

W

V

L

-

P

-

V

-

E

N

S

H

R

G

G

I

V

T

Q

G

-

P

-

L

V

G

G

M

A

P

N

V

M

T

S

I

L

S

G

V

I

R

R

P

P

E

E

R

H

I

L

A

L

L

-

T

-

R

-

K

-

P

P

P

S

E

D

G

I

A

A

Y

D

N

V

W

T

G

L

K

E

G

G

V

E

V

V

T

Q

N

V

I

V

A
x
E

Y

Q

M

L

G

G

G

H

Y

E

S

T

L

Q

Y

I

H

H

V

I

K

Q

L

I

L86 (= L113) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P187) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D192) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
E306 (≠ A335) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

8hplC Lpqy-sugabc in state 1 (see paper)
46% identity, 61% coverage: 33:267/388 of query aligns to 4:240/384 of 8hplC

query
sites
8hplC

I

V

V

L

D

D

V

R

V

V

K

T

K

K

F

S

G
x
Y

E

P

T

R

V

A

A

A

V

V

K

K

Q

E

V

F

N

S

L

M

S

T

V

I

K

A

K

D

G

G

E

E

L

F

F

I

A

I

L

L

L

V

G

G

S

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

T

L

L

R

N

M

M

L

I

A

A

G

G

L

L

E

E

T

E

V

I

T

T

S

S

G

G

K

E

I

L

L

R

I

I

D

G

G

G

E

E

D

R

L

V

A

N

Q

E

L

K

P

A

P

P

Y

K

R

D

R

R

P

D

V

I

N

A

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

E

R

S

Q

N

N

V

I

A

A

F

F

G

P

L

L

K

T

Q

L

E

A

G

K

V

V

P

P

K

K

A

A

E

E

L

I

K

A

D

A

R

K

V

V

Q

E

T

E

A

T

L

A

E

K

L

I

V

L

Q

D

M

L

G

S

R

E

F

L

A

L

N

D

R

R

K

K

P

P

H

G

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

S

A

L

I

V

V

K

R

R

S

P

P

K

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

K

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

A

E

E

L

I

V

S

N

R

I

L

L

Q

D

D

T

R

V

L

G

G

V

T

T

T

C

T

I

V

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

T

E

E

A

A

M

M

T

T

M

L

A

G

G

D

R

R

L

V

A

V

V

V

M

M

S

L

E

A

G

G

E

E

I

V

V

Q

Q

Q

L

I

G

G

T

T

P

P

H

D

E

E

V

L

Y

Y

E

S

Y

S

P

P

N

A

S

N

R

L

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

A

T

M

N

N

L

F

F

F

binding adenosine-5'-triphosphate: Y12 (≠ G41), G37 (= G66), C38 (= C67), G39 (= G68), K40 (= K69), S41 (= S70), T42 (= T71)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
41% identity, 82% coverage: 31:347/388 of query aligns to 7:311/353 of 1vciA

query
sites
1vciA

V

V

Q

K

I

L

V

E

D

N

V

L

V

T

K

K

K

R

F
|
F

G

G

E

N

T
x
F

V

T

A

A

V

V

K

N

Q

K

V

L

N

N

L

L

S

T

V

I

K

K

K

D

G

G

E

E

L

F

F

L

A

V

L

L

G

G

S

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

R

M

M

L

I

A

A

G

G

L

L

E

E

T

E

V

P

T

T

S

E

G

G

K

R

I

I

L

Y

I

F

D

G

G

D

E

R

D

D

L

V

A

T

Q

Y

L

L

P

P

Y

K

R

D

R

R

P

N

V

I

N

S

M

M

M

V

F

F

Q

Q

S

-

Y

-

A

-

L

-

F

-

P

-

H

H

M

M

T

T

V

V

E

Y

S

E

N

N

V

I

A

A

F

F

G

P

L

L

K

K

Q

K

E

-

G

-

V

F

P

P

K

K

A

D

E

E

L

I

K

D

D

K

R

R

V

V

Q

R

T

W

A

A

L

A

E

E

L

L

V

L

Q

Q

M

I

G

E

R

E

F

L

A

L

N

N

R

R

K

Y

P

P

H

A

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

L

V

A

A

R

R

S

A

L

I

V

V

K

V

R

E

P

P

K

D

L

V

L

L

L

M

D

D

E

E

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

K

I

L

R

R

Q

V

R

A

T

M

Q

R

I

A

E

E

L

I

V

K

N

K

I

L

L

Q

D

Q

T

K

V

L

G

K

V

V

T

T

C

T

I

I

M

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

M

A

G

G

D

R

R

L

I

A

A

V

V

M

M

S

N

E

R

G

G

E

Q

I

L

V

L

Q

Q

L

I

G

G

T

S

P

P

H

T

E

E

V

V

Y

Y

E

L

Y

R

P

P

N

N

S

S

R

V

F

F

S

V

A

A

E

T

F

F

I

I

G

G

S

A

T

P

N

-

L

-

F

-

E

E

G

M

H

N

T

I

V

L

E

E

D

V

E

S

P

V

D

G

H

D

V

G

F

Y

I

L

E

E

T

G

P

R

D

G

L

F

T

R

C

I

R

E

L

L

Y

P

V

Q

N

M

H

D

G

L

I

L

T

K

G

D

P

Y

L

V

G

G

M

K

P

T

V

V

T

L

I

F

S

G

V

I

R

R

P

P

E

E

R

H

I

M

A

T

L

V

T

-

R

-

K

-

P

-

E

E

G

G

A

V

Y

H

N

M

W

K

G

R

K

T

-

A

-

R

-

L

-

I

G

G

V

K

V

V

T

D

N

F

I

V

A

E

Y

A

M

L

G

G

G

T

Y

D

S

T

L

I

Y

L

H

H

V

V

K

K

L

F

binding adenosine-5'-triphosphate: F16 (= F40), F19 (≠ T43), S41 (= S65), G42 (= G66), G44 (= G68), K45 (= K69), T46 (≠ S70), T47 (= T71)

8hprC Lpqy-sugabc in state 4 (see paper)
48% identity, 57% coverage: 45:267/388 of query aligns to 18:242/363 of 8hprC

query
sites
8hprC

A

A

V

V

K

K

Q

E

V

F

N

S

L

M

S

T

V

I

K

A

K

D

G

G

E

E

L

F

F

I

A

I

L

L

L

V

G

G

S

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T

T

L

T

L

L

R

N

M

M

L

I

A

A

G

G

L

L

E

E

T

E

V

I

T

T

S

S

G

G

K

E

I

L

L

R

I

I

D

G

G

G

E

E

D

R

L

V

A

N

Q

E

L

K

P

A

P

P

Y

K

R

D

R

R

P

D

V

I

N

A

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

E

R

S

Q

N

N

V

I

A

A

F

F

G

P

L

L

K

T

Q

L

E

A

G

K

V

V

P

P

K

K

A

A

E

E

L

I

K

A

D

A

R

K

V

V

Q

E

T

E

A

T

L

A

E

K

L

I

V

L

Q

D

M

L

G

S

R

E

F

L

A

L

N

D

R

R

K

K

P

P

H

G

Q
|
Q

L

L

S

S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

S

A

L

I

V

V

K

R

R

S

P

P

K

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

M

L

S

S

A

N

L

L

D

D

K

A

Q

K

I

L

R

R

Q

V

R

Q

T

M

Q

R

I

A

E

E

L

I

V

S

N

R

I

L

L

Q

D

D

T

R

V

L

G

G

V

T

T

T

C

T

I

V

M

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

M

M

T

T

M

L

A

G

G

D

R

R

L

V

A

V

V

V

M

M

S

L

E

A

G

G

E

E

I

V

V

Q

Q

Q

L

I

G

G

T

T

P

P

H

D

E

E

V

L

Y

Y

E

S

Y

S

P

P

N

A

S

N

R

L

F

F

S

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

A

T

M

N

N

L

F

F

F

binding adenosine-5'-triphosphate: S38 (= S65), G39 (= G66), G41 (= G68), K42 (= K69), S43 (= S70), Q82 (= Q109), Q133 (= Q160), G136 (= G163), G137 (= G164), Q138 (= Q165), H192 (= H219)
binding magnesium ion: S43 (= S70), Q82 (= Q109)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprD Lpqy-sugabc in state 4 (see paper)
48% identity, 57% coverage: 45:267/388 of query aligns to 18:242/362 of 8hprD

query
sites
8hprD

A

A

V

V

K

K

Q

E

V

F

N

S

L

M

S

T

V

I

K

A

K

D

G

G

E

E

L

F

F

I

A

I