SitesBLAST

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
26% identity, 56% coverage: 57:296/429 of query aligns to 115:355/495 of Q9NRA2

query
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Q9NRA2

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K136 (= K78) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ L123) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AN 138:139) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ LA 206:207) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ QTLRA 208:212) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G269) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P275) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
371 G → V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
27% identity, 56% coverage: 57:296/429 of query aligns to 115:355/495 of Q8BN82

query
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Q8BN82

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H183 (≠ L123) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
27% identity, 56% coverage: 57:296/429 of query aligns to 115:355/495 of Q5Q0U0

query
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Q5Q0U0

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K136 (= K78) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R108) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L111) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G112) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E115) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A116) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ M119) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P120) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ L123) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ I126) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLK-N 268:272 (≠ QTLRAE 208:213) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P275) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
371 G→V: Remains in the endoplasmic reticulum.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
22% identity, 73% coverage: 8:319/429 of query aligns to 25:351/448 of Q51955

query
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Q51955

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D41 (≠ A24) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D27) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (≠ A69) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (≠ E73) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (≠ S76) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R108) mutation to A: Abolishes 4-HBA transport.
E144 (≠ N128) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ R167) mutation to A: Decrease in 4-HBA transport and chemotaxis.
D323 (= D291) mutation to N: Abolishes 4-HBA transport and chemotaxis.
H328 (≠ R296) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.

Sites not aligning to the query:

386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 88% coverage: 52:428/429 of query aligns to 126:517/582 of Q9JI12

query
sites
Q9JI12

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H128 (≠ G54) mutation to A: Greatly lowers L-glutamate transport.
R184 (= R108) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E115) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ W242) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
21% identity, 86% coverage: 52:421/429 of query aligns to 68:452/452 of 7t3nA

query
sites
7t3nA

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binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y61), Y137 (≠ M119), Y165 (≠ P147), R264 (≠ W242), F268 (≠ V246), Y269 (= Y247)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E134), G163 (= G145)

Sites not aligning to the query:

binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: 12, 13

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
23% identity, 55% coverage: 23:257/429 of query aligns to 112:346/570 of Q9C0U9

query
sites
Q9C0U9

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Sites not aligning to the query:

14 modified: Phosphoserine

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
21% identity, 76% coverage: 13:337/429 of query aligns to 92:421/534 of P53322

query
sites
P53322

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Y

K283 (≠ Q201) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 97% coverage: 9:424/429 of query aligns to 19:446/452 of Q5EXK5

query
sites
Q5EXK5

R

R

W

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D82 (≠ E73) mutation to A: Loss of activity.
V311 (≠ W288) mutation to W: Loss of activity.
D314 (= D291) mutation to A: Loss of activity.

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
22% identity, 46% coverage: 104:299/429 of query aligns to 166:361/497 of Q9Y2C5

query
sites
Q9Y2C5

L

L

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Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
372 A → T: in dbSNP:rs11754288

P77589 3-(3-hydroxy-phenyl)propionate transporter; 3HPP transporter; 3-(3-hydroxy-phenyl)propionate:H(+) symporter; 3HPP:H(+) symporter from Escherichia coli (strain K12) (see paper)
24% identity, 79% coverage: 68:405/429 of query aligns to 70:390/403 of P77589

query
sites
P77589

G

G

A

G

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Y

L

A

A

E
x
D

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K

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A

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A

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K

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-

L

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L

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G

G

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A

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L

A

A

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F

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I

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L

D75 (≠ E73) mutation D->A,E: Lack of 3HPP transport activity.
A272 (≠ S287) mutation to H: 30% increase in 3HPP transport activity.
K276 (= K292) mutation to D: Lack of 3HPP transport activity.

Sites not aligning to the query:

27 E→A: Lack of 3HPP transport activity.; E→D: Slight decrease in 3HPP transport activity.

Query Sequence

>H281DRAFT_05212 H281DRAFT_05212 Sugar phosphate permease
MTSSLAIRRWWTIMPIVFITYSLAYLDRANYGFAAAAGINQDLGISKGLSSLIGALFFLG
YFFFQIPGAIYAERRSVKKLVFWSLVLWGGCAALTGMVSNIPSLMVIRFVLGVVEAAVMP
AMLIFISNWFTKSERSRANTFLILGNPVTVLWMSVVSGYLVHSFGWRNMFIAEGIPAILW
AVCWWFIVQDKPQQVSWLTQQQKDDLAQTLRAEQAAIKPVRNYSEAFRSSAVIKLCAQYF
CWSIGVYGFVLWLPSILKNGSSLGMVETGWLSALPYLAATIAMLAASWASDKLNRRKVFV
WPFLLIGAIAFAASYALGSTHFWLSYASLVIAGAAMYAPYGPFFAIVPELLPKNVAGGAM
ALINSMGALGSFVGSYVVGYLNGATGSPAASYAFMSVALIAAVILTLIVKPQQQTVETPH
KLATPLQGK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory