SitesBLAST
Comparing H281DRAFT_05256 FitnessBrowser__Burk376:H281DRAFT_05256 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
55% identity, 85% coverage: 43:331/341 of query aligns to 11:295/494 of 1tdjA
- active site: K58 (= K90), A83 (= A115), E209 (≠ Q245), S213 (= S249), C215 (≠ A251), G237 (= G273)
- binding pyridoxal-5'-phosphate: F57 (= F89), K58 (= K90), N85 (= N117), G184 (= G220), G185 (= G221), G186 (= G222), G187 (= G223), G237 (= G273), E282 (= E318)
Sites not aligning to the query:
P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
55% identity, 85% coverage: 43:331/341 of query aligns to 15:299/514 of P04968
- K62 (= K90) modified: N6-(pyridoxal phosphate)lysine
- N89 (= N117) binding
- GGGGL 188:192 (= GGGGL 220:224) binding
Sites not aligning to the query:
2gn2A Crystal structure of tetrameric biodegradative threonine deaminase (tdcb) from salmonella typhimurium in complex with cmp at 2.5a resolution (hexagonal form) (see paper)
38% identity, 80% coverage: 46:318/341 of query aligns to 16:280/326 of 2gn2A
- active site: K56 (= K90), A81 (= A115), Q207 (= Q245), V211 (≠ S249), G213 (≠ A251), G235 (= G273)
- binding cytidine-5'-monophosphate: R51 (≠ P85), T52 (≠ V86), G53 (≠ F87), A114 (= A148), D117 (≠ A151), Y118 (≠ H152)
Sites not aligning to the query:
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
33% identity, 85% coverage: 50:340/341 of query aligns to 13:297/319 of 2zr8A
- active site: K53 (= K90), S78 (≠ A115), E204 (≠ Q245), G208 (≠ S249), D210 (≠ A251), G232 (= G273)
- binding magnesium ion: E204 (≠ Q245), G208 (≠ S249), D210 (≠ A251)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F89), K53 (= K90), S77 (= S114), S78 (≠ A115), N80 (= N117), H81 (= H118), P147 (= P187), G179 (= G220), G180 (= G221), G181 (= G222), G182 (= G223), G232 (= G273), E277 (= E318), T279 (≠ A320)
- binding serine: S78 (≠ A115), R129 (≠ A169), D231 (= D272), G232 (= G273), A233 (≠ T274), Q234 (≠ A275), T235 (≠ V276)
Sites not aligning to the query:
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
33% identity, 85% coverage: 50:340/341 of query aligns to 13:297/319 of 2zpuA
- active site: K53 (= K90), S78 (≠ A115), E204 (≠ Q245), G208 (≠ S249), D210 (≠ A251), G232 (= G273)
- binding magnesium ion: E204 (≠ Q245), G208 (≠ S249), D210 (≠ A251)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F89), K53 (= K90), S77 (= S114), S78 (≠ A115), N80 (= N117), H81 (= H118), P147 (= P187), G179 (= G220), G180 (= G221), G181 (= G222), G182 (= G223), G232 (= G273), E277 (= E318), T279 (≠ A320)
Sites not aligning to the query:
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 85% coverage: 50:340/341 of query aligns to 17:301/323 of O59791
- S82 (≠ A115) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
33% identity, 85% coverage: 50:340/341 of query aligns to 12:296/318 of 1wtcA
- active site: K52 (= K90), S77 (≠ A115), E203 (≠ Q245), G207 (≠ S249), D209 (≠ A251), G231 (= G273)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ R58), K47 (≠ P85), M48 (≠ V86), A109 (≠ D147), A110 (= A148), Y114 (≠ H152)
- binding magnesium ion: E203 (≠ Q245), G207 (≠ S249), D209 (≠ A251)
- binding pyridoxal-5'-phosphate: F51 (= F89), K52 (= K90), N79 (= N117), G178 (= G220), G179 (= G221), G180 (= G222), G181 (= G223), G231 (= G273), E276 (= E318), T278 (≠ A320)
Sites not aligning to the query:
1v71A Crystal structure of s.Pombe serine racemase
33% identity, 85% coverage: 50:340/341 of query aligns to 12:296/318 of 1v71A
- active site: K52 (= K90), S77 (≠ A115), E203 (≠ Q245), G207 (≠ S249), D209 (≠ A251), G231 (= G273)
- binding magnesium ion: E203 (≠ Q245), G207 (≠ S249), D209 (≠ A251)
- binding pyridoxal-5'-phosphate: F51 (= F89), K52 (= K90), N79 (= N117), G178 (= G220), G179 (= G221), G180 (= G222), G181 (= G223), G231 (= G273), E276 (= E318), T278 (≠ A320)
Sites not aligning to the query:
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
33% identity, 82% coverage: 51:328/341 of query aligns to 29:301/339 of Q7XSN8
- E219 (≠ Q245) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (≠ A251) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
32% identity, 86% coverage: 42:334/341 of query aligns to 7:293/319 of A4F2N8
- K53 (= K90) mutation to A: Loss of enzymatic activity.
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
35% identity, 74% coverage: 73:326/341 of query aligns to 37:288/320 of 7nbhAAA
- active site: K53 (= K90), S81 (≠ A115), E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249), G236 (= G273)
- binding calcium ion: E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ A115), G85 (≠ A119), Q86 (= Q120), K111 (= K145), I115 (≠ V149), Y118 (≠ H152), D235 (= D272), P281 (= P319)
Sites not aligning to the query:
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
35% identity, 74% coverage: 73:326/341 of query aligns to 37:288/322 of 7nbgAAA
- active site: K53 (= K90), S81 (≠ A115), E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249), G236 (= G273)
- binding calcium ion: E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249)
- binding pyridoxal-5'-phosphate: F52 (= F89), K53 (= K90), N83 (= N117), G182 (= G220), G183 (= G221), G184 (= G222), G185 (= G223), M186 (≠ L224), G236 (= G273), V237 (≠ T274), T282 (≠ A320)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ A115), G85 (≠ A119), Q86 (= Q120), I101 (= I135), K111 (= K145), I115 (≠ V149), Y118 (≠ H152)
Sites not aligning to the query:
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
35% identity, 74% coverage: 73:326/341 of query aligns to 37:288/323 of 7nbfAAA
- active site: K53 (= K90), S81 (≠ A115), E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249), G236 (= G273)
- binding calcium ion: E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249)
- binding magnesium ion: N244 (≠ E282)
- binding pyridoxal-5'-phosphate: F52 (= F89), K53 (= K90), N83 (= N117), G182 (= G220), G183 (= G221), G184 (= G222), G185 (= G223), M186 (≠ L224), G236 (= G273), V237 (≠ T274), T282 (≠ A320)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: F46 (≠ N83)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
35% identity, 74% coverage: 73:326/341 of query aligns to 37:288/323 of 7nbdAAA
- active site: K53 (= K90), S81 (≠ A115), E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249), G236 (= G273)
- binding calcium ion: E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ F310), L278 (≠ V316)
- binding magnesium ion: N244 (≠ E282)
- binding pyridoxal-5'-phosphate: F52 (= F89), K53 (= K90), N83 (= N117), G182 (= G220), G183 (= G221), G184 (= G222), G185 (= G223), M186 (≠ L224), G236 (= G273), V237 (≠ T274), E280 (= E318), T282 (≠ A320)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
35% identity, 74% coverage: 73:326/341 of query aligns to 37:288/323 of 7nbcCCC
- active site: K53 (= K90), S81 (≠ A115), E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249), G236 (= G273)
- binding biphenyl-4-ylacetic acid: T78 (= T112), H79 (≠ A113), H84 (= H118), V148 (= V185), H149 (= H186), P150 (= P187)
- binding calcium ion: E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249)
- binding pyridoxal-5'-phosphate: F52 (= F89), K53 (= K90), N83 (= N117), G182 (= G220), G183 (= G221), G184 (= G222), G185 (= G223), M186 (≠ L224), G236 (= G273), V237 (≠ T274), T282 (≠ A320)
Sites not aligning to the query:
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
35% identity, 74% coverage: 73:326/341 of query aligns to 37:288/323 of 7nbcAAA
- active site: K53 (= K90), S81 (≠ A115), E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249), G236 (= G273)
- binding calcium ion: E207 (≠ Q245), A211 (vs. gap), D213 (≠ S249)
- binding magnesium ion: N244 (≠ E282)
- binding pyridoxal-5'-phosphate: F52 (= F89), K53 (= K90), N83 (= N117), G182 (= G220), G183 (= G221), G184 (= G222), G185 (= G223), M186 (≠ L224), G236 (= G273), V237 (≠ T274), T282 (≠ A320)
Sites not aligning to the query:
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
40% identity, 74% coverage: 81:334/341 of query aligns to 41:290/308 of 1ve5A
- active site: K50 (= K90), S56 (≠ N96), S72 (≠ A115), E200 (≠ Q245), A204 (≠ S249), D206 (≠ A251), G229 (= G273)
- binding calcium ion: E200 (≠ Q245), A204 (≠ S249), D206 (≠ A251)
- binding pyridoxal-5'-phosphate: F49 (= F89), K50 (= K90), N74 (= N117), G175 (= G220), G176 (= G221), G177 (= G222), G178 (= G223), E274 (= E318), T276 (≠ A320)
Sites not aligning to the query:
5cvcA Structure of maize serine racemase (see paper)
33% identity, 82% coverage: 51:328/341 of query aligns to 13:285/329 of 5cvcA
- active site: K52 (= K90), S77 (≠ A115), E203 (≠ Q245), A207 (≠ S249), D209 (≠ A251), G231 (= G273)
- binding magnesium ion: E203 (≠ Q245), A207 (≠ S249), D209 (≠ A251)
- binding pyridoxal-5'-phosphate: F51 (= F89), K52 (= K90), N79 (= N117), S178 (≠ G220), G179 (= G221), G180 (= G222), G181 (= G223), L232 (≠ T274), E275 (= E318)
Sites not aligning to the query:
6zspAAA serine racemase bound to atp and malonate. (see paper)
34% identity, 76% coverage: 67:326/341 of query aligns to 33:281/320 of 6zspAAA
- active site: K53 (= K90), S74 (≠ A115), E200 (≠ Q245), A204 (vs. gap), D206 (≠ S249), G229 (= G273)
- binding adenosine-5'-triphosphate: K48 (≠ P85), T49 (≠ V86), Q79 (= Q120), Y111 (≠ H152), E266 (≠ Q311), R267 (≠ D312), K269 (≠ R314)
- binding magnesium ion: E200 (≠ Q245), A204 (vs. gap), D206 (≠ S249)
- binding malonate ion: K53 (= K90), S73 (= S114), S74 (≠ A115), N76 (= N117), H77 (= H118), R125 (≠ Y166), G229 (= G273), S232 (≠ V276)
Sites not aligning to the query:
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
34% identity, 74% coverage: 73:326/341 of query aligns to 38:284/322 of 3l6bA
- active site: K54 (= K90), S77 (≠ A115), E203 (≠ Q245), A207 (vs. gap), D209 (≠ S249), G232 (= G273), T278 (≠ A320)
- binding malonate ion: K54 (= K90), S76 (= S114), S77 (≠ A115), N79 (= N117), H80 (= H118), R128 (≠ Y166), G232 (= G273)
- binding manganese (ii) ion: E203 (≠ Q245), A207 (vs. gap), D209 (≠ S249)
- binding pyridoxal-5'-phosphate: F53 (= F89), K54 (= K90), N79 (= N117), G178 (= G220), G179 (= G221), G180 (= G222), G181 (= G223), M182 (≠ L224), V233 (≠ T274), E276 (= E318), T278 (≠ A320)
Sites not aligning to the query:
Query Sequence
>H281DRAFT_05256 FitnessBrowser__Burk376:H281DRAFT_05256
MSACSRLLVAFRCYALAAQELALPSHHLEQVAETADSVALRHDYLKKTLTARVYDVARET
ELERAPQLSARLRNPVYLKREDNQPVFSFKVRGAYNKMAHIPAEALTRGVITASAGNHAQ
GVALSAARMGVKAIIVVPVTTPQVKVDAVRAHGGPTVEVVQFGESYSDAYGHAVQLQEER
GLTFVHPFDDPYVIAGQGTVAMEILSQHQGPIHAIFVPIGGGGLAAGVAAYVKSVRPEIK
VIGVQTDDSCAMAASLKAGERVTLNEVGLFSDGTAVKLVGEETFRLCSEYLDDVLLVNTD
ALCAAIKDVFQDTRSVLEPAGSLAVAGAKQYAEREGVENQT
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory