SitesBLAST
Comparing H281DRAFT_05516 FitnessBrowser__Burk376:H281DRAFT_05516 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
36% identity, 99% coverage: 4:264/264 of query aligns to 6:259/259 of 5zaiC
- active site: A65 (= A64), F70 (≠ Y69), S82 (≠ R82), R86 (≠ F91), G110 (= G115), E113 (= E118), P132 (≠ Q137), E133 (≠ D138), I138 (≠ F143), P140 (≠ A145), G141 (≠ C146), A226 (≠ R231), F236 (≠ G241)
- binding coenzyme a: K24 (≠ R22), L25 (≠ M23), A63 (= A62), G64 (= G63), A65 (= A64), D66 (= D65), I67 (≠ L66), P132 (≠ Q137), R166 (= R171), F248 (= F253), K251 (≠ R256)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
38% identity, 98% coverage: 4:262/264 of query aligns to 6:256/258 of 1mj3A
- active site: A68 (= A64), M73 (≠ Y69), S83 (≠ T86), L85 (≠ G88), G109 (= G115), E112 (= E118), P131 (≠ Q137), E132 (≠ D138), T137 (≠ F143), P139 (≠ A145), G140 (≠ C146), K225 (≠ R231), F235 (≠ G241)
- binding hexanoyl-coenzyme a: K26 (≠ E21), A27 (≠ R22), L28 (≠ M23), A30 (≠ S25), A66 (= A62), G67 (= G63), A68 (= A64), D69 (= D65), I70 (≠ L66), G109 (= G115), P131 (≠ Q137), E132 (≠ D138), L135 (≠ W141), G140 (≠ C146)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
38% identity, 98% coverage: 4:262/264 of query aligns to 6:258/260 of 2hw5C
- active site: A68 (= A64), M73 (≠ T68), S83 (≠ A78), L87 (≠ R82), G111 (= G115), E114 (= E118), P133 (≠ Q137), E134 (≠ D138), T139 (≠ F143), P141 (≠ A145), G142 (≠ C146), K227 (≠ R231), F237 (≠ G241)
- binding crotonyl coenzyme a: K26 (≠ E21), A27 (≠ R22), L28 (≠ M23), A30 (≠ S25), K62 (= K58), I70 (≠ L66), F109 (≠ I113)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
38% identity, 98% coverage: 4:262/264 of query aligns to 4:256/258 of 1ey3A
- active site: A66 (= A64), M71 (≠ T68), S81 (≠ A78), L85 (≠ R82), G109 (= G115), E112 (= E118), P131 (≠ Q137), E132 (≠ D138), T137 (≠ F143), P139 (≠ A145), G140 (≠ C146), K225 (≠ R231), F235 (≠ G241)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E21), L26 (≠ M23), A28 (≠ S25), A64 (= A62), G65 (= G63), A66 (= A64), D67 (= D65), I68 (≠ L66), L85 (≠ R82), W88 (≠ Y85), G109 (= G115), P131 (≠ Q137), L135 (≠ W141), G140 (≠ C146)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
38% identity, 98% coverage: 4:262/264 of query aligns to 6:258/260 of 1dubA
- active site: A68 (= A64), M73 (≠ T68), S83 (≠ A78), L87 (≠ R82), G111 (= G115), E114 (= E118), P133 (≠ Q137), E134 (≠ D138), T139 (≠ F143), P141 (≠ A145), G142 (≠ C146), K227 (≠ R231), F237 (≠ G241)
- binding acetoacetyl-coenzyme a: K26 (≠ E21), A27 (≠ R22), L28 (≠ M23), A30 (≠ S25), A66 (= A62), A68 (= A64), D69 (= D65), I70 (≠ L66), Y107 (≠ F111), G110 (≠ S114), G111 (= G115), E114 (= E118), P133 (≠ Q137), E134 (≠ D138), L137 (≠ W141), G142 (≠ C146), F233 (= F237), F249 (= F253)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
38% identity, 98% coverage: 4:262/264 of query aligns to 5:252/254 of 2dubA
- active site: A67 (= A64), M72 (≠ Y69), S82 (≠ T86), G105 (= G115), E108 (= E118), P127 (≠ Q137), E128 (≠ D138), T133 (≠ F143), P135 (≠ A145), G136 (≠ C146), K221 (≠ R231), F231 (≠ G241)
- binding octanoyl-coenzyme a: K25 (≠ E21), A26 (≠ R22), L27 (≠ M23), A29 (≠ S25), A65 (= A62), A67 (= A64), D68 (= D65), I69 (≠ L66), K70 (= K67), G105 (= G115), E108 (= E118), P127 (≠ Q137), E128 (≠ D138), G136 (≠ C146), A137 (≠ D147)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
35% identity, 100% coverage: 1:264/264 of query aligns to 4:261/261 of 5jbxB
- active site: A67 (= A64), R72 (≠ Y69), L84 (≠ T86), R88 (vs. gap), G112 (= G115), E115 (= E118), T134 (≠ Q137), E135 (≠ D138), I140 (≠ F143), P142 (≠ A145), G143 (≠ C146), A228 (≠ R231), L238 (≠ G241)
- binding coenzyme a: S24 (≠ E21), R25 (= R22), R26 (≠ M23), A28 (≠ S25), A65 (= A62), D68 (= D65), L69 (= L66), K70 (= K67), L110 (≠ I113), G111 (≠ S114), T134 (≠ Q137), E135 (≠ D138), L138 (≠ W141), R168 (= R171)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
38% identity, 98% coverage: 4:262/264 of query aligns to 36:288/290 of P14604
- E144 (= E118) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ D138) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
36% identity, 98% coverage: 4:261/264 of query aligns to 6:254/255 of 3q0jC
- active site: A65 (= A64), M70 (≠ Y69), T80 (≠ A78), F84 (= F91), G108 (= G115), E111 (= E118), P130 (≠ Q137), E131 (≠ D138), V136 (≠ F143), P138 (≠ A145), G139 (≠ C146), L224 (≠ R231), F234 (≠ G241)
- binding acetoacetyl-coenzyme a: Q23 (≠ E21), A24 (≠ R22), L25 (≠ M23), A27 (≠ S25), A63 (= A62), G64 (= G63), A65 (= A64), D66 (= D65), I67 (≠ L66), K68 (= K67), M70 (≠ Y69), F84 (= F91), G107 (≠ S114), G108 (= G115), E111 (= E118), P130 (≠ Q137), E131 (≠ D138), P138 (≠ A145), G139 (≠ C146), M140 (≠ D147)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
36% identity, 98% coverage: 4:261/264 of query aligns to 6:254/255 of 3q0gC
- active site: A65 (= A64), M70 (≠ Y69), T80 (≠ A78), F84 (= F91), G108 (= G115), E111 (= E118), P130 (≠ Q137), E131 (≠ D138), V136 (≠ F143), P138 (≠ A145), G139 (≠ C146), L224 (≠ R231), F234 (≠ G241)
- binding coenzyme a: L25 (≠ M23), A63 (= A62), I67 (≠ L66), K68 (= K67), Y104 (≠ F111), P130 (≠ Q137), E131 (≠ D138), L134 (≠ W141)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
36% identity, 98% coverage: 4:261/264 of query aligns to 5:253/256 of 3h81A
- active site: A64 (= A64), M69 (≠ Y69), T79 (≠ A78), F83 (= F91), G107 (= G115), E110 (= E118), P129 (≠ Q137), E130 (≠ D138), V135 (≠ F143), P137 (≠ A145), G138 (≠ C146), L223 (≠ R231), F233 (≠ G241)
- binding calcium ion: F233 (≠ G241), Q238 (≠ L246)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
36% identity, 98% coverage: 4:261/264 of query aligns to 5:249/250 of 3q0gD
- active site: A64 (= A64), M69 (≠ Y69), T75 (≠ A78), F79 (= F91), G103 (= G115), E106 (= E118), P125 (≠ Q137), E126 (≠ D138), V131 (≠ F143), P133 (≠ A145), G134 (≠ C146), L219 (≠ R231), F229 (≠ G241)
- binding Butyryl Coenzyme A: F225 (= F237), F241 (= F253)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
35% identity, 99% coverage: 4:264/264 of query aligns to 5:257/257 of 6slbAAA
- active site: Q64 (≠ A64), F69 (≠ Y69), L80 (vs. gap), N84 (≠ T86), A108 (≠ G115), S111 (≠ E118), A130 (≠ Q137), F131 (≠ D138), L136 (≠ F143), P138 (≠ A145), D139 (≠ C146), A224 (≠ R231), G234 (= G241)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K58), A62 (= A62), Q64 (≠ A64), D65 (= D65), L66 (= L66), Y76 (≠ F81), A108 (≠ G115), F131 (≠ D138), D139 (≠ C146)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
34% identity, 99% coverage: 4:264/264 of query aligns to 2:245/245 of 6slaAAA
- active site: Q61 (≠ A64), L68 (≠ F81), N72 (≠ Y85), A96 (≠ G115), S99 (≠ E118), A118 (≠ Q137), F119 (≠ D138), L124 (≠ F143), P126 (≠ A145), N127 (≠ C146), A212 (≠ R231), G222 (= G241)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ M23), A59 (= A62), Q61 (≠ A64), D62 (= D65), L63 (= L66), L68 (≠ F81), Y71 (≠ K84), A94 (≠ I113), G95 (≠ S114), A96 (≠ G115), F119 (≠ D138), I122 (≠ W141), L124 (≠ F143), N127 (≠ C146), F234 (= F253), K237 (≠ R256)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
29% identity, 99% coverage: 3:264/264 of query aligns to 9:266/266 of O53561
- K135 (≠ E133) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 133:140, 38% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (= K140) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
34% identity, 95% coverage: 11:262/264 of query aligns to 20:266/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
5du6A Crystal structure of m. Tuberculosis echa6 bound to ligand gsk059a. (see paper)
33% identity, 95% coverage: 12:263/264 of query aligns to 11:241/242 of 5du6A
- active site: A61 (= A64), P71 (= P79), I75 (≠ A92), A99 (≠ G115), Q102 (≠ E118), P121 (≠ Q137), T122 (≠ D138), L127 (≠ F143), L129 (≠ A145), D130 (≠ C146), P209 (≠ R231), W219 (≠ G241)
- binding (5R,7R)-5-(4-ethylphenyl)-N-(4-fluorobenzyl)-7-methyl-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrimidine-3-carboxamide: L74 (≠ F91), D82 (= D99), D130 (≠ C146), W132 (≠ G148), A207 (= A229), K212 (≠ S234), F215 (= F237)
3p85A Crystal structure enoyl-coa hydratase from mycobacterium avium (see paper)
37% identity, 72% coverage: 4:193/264 of query aligns to 3:170/224 of 3p85A
- active site: L62 (≠ A64), L67 (≠ Y69), P68 (= P79), G92 (= G115), E95 (= E118), T114 (≠ Q137), H115 (≠ D138), L120 (≠ F143), P122 (≠ A145), T123 (≠ C146)
- binding calcium ion: D43 (= D44), D45 (= D46)
Sites not aligning to the query:
4elwA Structure of e. Coli. 1,4-dihydroxy-2- naphthoyl coenzyme a synthases (menb) in complex with nitrate (see paper)
32% identity, 98% coverage: 3:261/264 of query aligns to 21:260/267 of 4elwA
- active site: G83 (≠ A64), L91 (≠ P79), G115 (= G115), V118 (≠ E118), G138 (≠ D138), S143 (≠ F143), D145 (≠ A145), G146 (≠ C146), A232 (vs. gap), Y240 (≠ G241)
- binding nitrate ion: G114 (≠ S114), T137 (≠ Q137), G138 (≠ D138), F144 (≠ H144), W166 (≠ I166)
5dufA Crystal structure of m. Tuberculosis echa6 bound to ligand gsk729a (see paper)
33% identity, 95% coverage: 12:263/264 of query aligns to 12:244/245 of 5dufA
- active site: A62 (= A64), D67 (≠ Y69), P74 (≠ R82), I78 (≠ T86), A102 (≠ G115), Q105 (≠ E118), P124 (≠ Q137), T125 (≠ D138), L130 (≠ F143), L132 (≠ A145), D133 (≠ C146), P212 (≠ R231), W222 (≠ G241)
- binding (5R,7S)-5-(4-ethylphenyl)-7-(trifluoromethyl)-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrimidine-3-carboxylic acid: L77 (≠ Y85), I78 (≠ T86), H81 (≠ T95), D85 (= D99), Q105 (≠ E118), D133 (≠ C146), W135 (≠ G148)
Query Sequence
>H281DRAFT_05516 FitnessBrowser__Burk376:H281DRAFT_05516
MADILYESHGHVRLIIINRAERMNSLNFEANDELVGAFESFNDDDDAYVAVVTGAGEKAF
CAGADLKTYTVNFARSPAPDFRRKYTNGPGFAGITRNMDIFKPVVAAVNGFAISGGFELA
LAADLRFCSPNAEFALQDAKWGFHACDGGLIRLPQIVGLGHAMEIILSGERLNAEHAYRI
GLVNRIVPAESLLEETMRYAQMLASRSPLSHRFAKEVMKRAIGMPMDEALRLESRSFYDF
GMTEDLVEGTTSFRERRPANFKGK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory