SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_05849 FitnessBrowser__Burk376:H281DRAFT_05849 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1cjxA Crystal structure of pseudomonas fluorescens hppd (see paper)
57% identity, 98% coverage: 6:361/365 of query aligns to 1:351/352 of 1cjxA

query
sites
1cjxA

W

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binding acetate ion: H237 (= H252), F334 (= F344)
binding fe (ii) ion: H158 (= H173), H237 (= H252), E319 (= E329)

7xntA Crystal structure of pfhppd-y13161 complex
57% identity, 96% coverage: 6:355/365 of query aligns to 4:341/341 of 7xntA

query
sites
7xntA

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binding 3-(2,6-dimethylphenyl)-1-methyl-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-quinazoline-2,4-dione: H161 (= H173), T163 (= T175), S201 (= S213), P214 (= P226), Q221 (= Q237), H236 (= H252), F304 (= F318), F313 (= F327), F325 (= F339), F330 (= F344)
binding cobalt (ii) ion: H161 (= H173), H236 (= H252), E315 (= E329)

7x8eA Crystal structure of pfhppd-y13287 complex
56% identity, 97% coverage: 6:359/365 of query aligns to 3:341/341 of 7x8eA

query
sites
7x8eA

W

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binding 1,5-dimethyl-3-(2-methylphenyl)-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-quinazoline-2,4-dione: S193 (= S213), P206 (= P226), Q214 (= Q237), H229 (= H252), M252 (≠ L275), F300 (= F318), F321 (= F339), F326 (= F344)
binding cobalt (ii) ion: H160 (= H173), H229 (= H252), E311 (= E329)

7xntC Crystal structure of pfhppd-y13161 complex
54% identity, 95% coverage: 6:350/365 of query aligns to 3:320/320 of 7xntC

query
sites
7xntC

W

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H

L

L

T
|
T

H

H

N

N

V

V

H

Y

R

R

G

G

R

R

M

M

Q

V

E

Y

W

W

A

A

E

N

F

F

Y

Y

E

E

R

K

L

L

F

F

N

N

F

F

R

R

E

E

V

-

R

-

Y

-

F

-

D

-

I

-

E

-

G

-

K

-

V

-

T

-

G

-

V

A

K

T

S

S

K

K

A

A

M

M

T

S

S

A

P

P

C

D

G

G

K

M

I

I

R

R

I

I

P
|
P

I

L

N

N

E

E

E

E

G

-

S

-

E

-

T

-

S

-

G

-

Q
|
Q

I

I

Q

E

E

E

Y

F

L

L

D

M

A

Q

Y

F

R

N

G

G

E

E

G

G

I

I

Q

Q

H
|
H

I

V

A

A

L

F

G

L

S

T

N

D

D

D

I

L

Y

V

R

K

T

T

V

W

D

D

G

A

L

L

R

K

A

K

A

I

N

G

I

M

S

R

L

F

L
x
M

D

T

T

A

-

P

I

P

D

D

T

T

Y

Y

Y

Y

E

E

L

M

V

L

D

E

R

G

R

R

V

L

P

P

N

D

H

H

G

G

E

E

P

P

L

V

D

D

E

Q

L

L

R

Q

K

A

R

R

K

G

I

I

L

L

I

L

D

D

G

G

A

S

P

S

D

K

D

R

L

L

L

L

L

L

Q

Q

I

I

F
|
F

T

S

E

E

N

T

Q

L

I

M

G

G

P

P

I

V

F

F

F

F

E
|
E

I

F

I

I

Q

Q

R

R

K

K

G

G

N

D

Q

D

G

G

F
|
F

G
|
G

E

E

G

G

N
|
N

F
|
F

K

K

A

A

L

L

F

F

E

E

S

S

binding 3-(2,6-dimethylphenyl)-1-methyl-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-quinazoline-2,4-dione: H160 (= H173), T162 (= T175), P201 (= P226), Q206 (= Q237), H221 (= H252), M244 (≠ L275), F288 (= F318), F309 (= F339), G310 (= G340), N313 (= N343), F314 (= F344)
binding cobalt (ii) ion: H160 (= H173), H221 (= H252), E299 (= E329)

5hmqD Xylose isomerase-like tim barrel/4-hydroxyphenylpyruvate dioxygenase fusion protein
36% identity, 91% coverage: 13:343/365 of query aligns to 296:611/624 of 5hmqD

query
sites
5hmqD

D

D

G

G

F

V

E

E

F

F

I

L

E

E

Y

F

T

A

A

V

P

D

D

E

P

A

-

V

-

G

K

A

A

R

L

L

G

G

K

N

L

W

F

L

E

K

Q

R

M

L

G

G

F

F

T

A

A

E

V

A

A

G

R

K

H

H

R

R

H

S

K

K

D

E

V

V

T

Q

L

L

Y

L

R

R

Q

Q

G

G

D

D

I

I

N

N

F

I

I

V

V

L

N

N

G

A

E

E

P

P

D

Y

S

S

F

F

A

G

Q

H

R

N

F

F

A

F

R

E

L

A

H

H

G

G

P

P

S

S

I

L

C

C

A

A

I

T

A

A

F

L

R

R

V

V

Q

K

D

D

A

Q

G

Q

K

A

A

A

Y

L

R

K

Q

R

A

A

V

T

E

A

K

F

G

R

A

G

W

Q

G

P

F

F

D

R

N

G

K

L

T

V

G

G

P

P

M

N

E

E

L

C

N

E

I

V

P

P

A

A

I

V

K

R

G

A

I

P

G

D

D

G

S

S

L

L

I

L

Y

Y

F

L

V

V

D

E

R

Q

W

-

R

-

G

-

K

-

N

-

G

-

A

-

A

-

P

-

G

-

S

-

V

-

G

G

D

T

I

H

D

T

I

L

Y

Y

D

D

V

T

D

D

F

F

E

S

P

-

I

-

P

L

G

D

A

N

N

N

P

A

N

T

P

A

V

T

G

G

H

-

G

G

L

L

T

R

Y

R

I

I

D

D

H
|
H

L

M

T

A

H

L

N

A

V

L

H

P

R

A

G

E

R

S

M

L

Q

D

E

S

W

W

A

V

E

L

F

F

Y

Y

E

K

R

S

L

L

F

F

N

D

F

F

R

A

E

A

V

-

R

-

Y

-

F

-

D

D

I

D

E

E

G

V

K

V

V

L

T

P

G

G

-

L

V

V

K

K

S

S

K

R

A

A

M

L

T

R

S

S

P

Q

C

C

G

G

K

T

I

L

R

R

I

L

P

P

I

L

N

N

E

I

E

S

G

E

S

N

E

R

T

N

S

T

G

A

Q

-

I

I

Q

A

E

H

Y

A

L

L

D

S

A

S

Y

Y

R

R

G

G

E

S

G

G

I

V

Q

H

H
|
H

I

I

A

A

L

F

G

D

S

C

N

D

D

D

I

I

Y

F

R

R

T

E

V

V

D

A

G

R

L

A

R

K

A

L

A

A

N

G

I

V

S

P

L

L

L

L

D

E

T

I

I

P

D

L

T

N

Y

Y

Y

Y

E

D

L

D

V

L

D

A

R

A

R

R

V

F

P

D

N

F

H

D

G

D

E

E

P

F

L

L

D

S

E

E

L

L

R

A

K

Y

R

Y

K

N

I

V

L

L

I

Y

D

D

-

R

G

D

A

A

P

Q

D

G

D

G

L

E

L

L

L

F

Q

H

I

V

F

Y

T

T

E

E

N

P

Q

F

I

E

G

E

P

R

I

F

F

F

F

F

E
|
E

I

I

I

I

Q

Q

R

R

K

K

-

A

G

G

N

Y

Q

A

G

G

F

Y

G

G

E

A

G

A

N

N

binding magnesium ion: H443 (= H173), H518 (= H252), E596 (= E329)

Sites not aligning to the query:

binding magnesium ion: 136, 167, 193, 241

Q88JU3 3-dehydroshikimate dehydratase; DSD; EC 4.2.1.118 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
35% identity, 91% coverage: 13:343/365 of query aligns to 294:614/635 of Q88JU3

query
sites
Q88JU3

D

D

G

G

F

V

E

E

F

F

I

L

E

E

Y

F

T

A

A

V

P

D

D

E

P

A

-

V

-

G

K

A

A

R

L

L

G

G

K

N

L

W

F

L

E

K

Q

R

M

L

G

G

F

F

T

A

A

E

V

A

A

G

R

K

H

H

R

R

H

S

K

K

D

E

V

V

T

Q

L

L

Y

L

R

R

Q

Q

G

G

D

D

I

I

N

N

F

I

I

V

V

L

N

N

G

A

E

E

P

P

D

Y

S

S

F

F

A

G

Q

H

R

N

F

F

A

F

R

E

L

A

H

H

G

G

P

P

S

S

I

L

C

C

A

A

I

T

A

A

F

L

R

R

V

V

Q

K

D

D

A

Q

G

Q

K

A

A

A

Y

L

R

K

Q

R

A

A

V

T

E

A

K

F

G

R

A

G

W

Q

G

P

F

F

D

R

N

G

K

L

T

V

G

G

P

P

M

N

E

E

L

C

N

E

I

V

P

P

A

A

I

V

K

R

G

A

I

P

G

D

D

G

S

S

L

L

I

L

Y

Y

F

L

V

V

D

E

R

Q

W

-

R

-

G

-

K

-

N

-

G

-

A

-

A

-

P

-

G

G

S

T

V

A

G

G

D

H

I

T

D

-

I

L

Y

Y

D

D

V

T

D

D

F

F

E

S

P

-

I

-

P

L

G

D

A

N

N

N

P

A

N

T

P

A

V

T

G

G

H

-

G

G

L

L

T

R

Y

R

I

I

D

D

H
|
H

L

M

T

A

H

L

N

A

V

L

H

P

R

A

G

E

R

S

M

L

Q

D

E

S

W

W

A

V

E

L

F

F

Y

Y

E

K

R

S

L

L

F

F

N

D

F

F

R

A

E

A

V

D

R

D

Y

E

F

V

D

V

I

L

E

P

G

D

K

P

V

Y

T

G

G

L

V

V

K

K

S

S

K

R

A

A

M

L

T

R

S

S

P

Q

C

C

G

G

K

T

I

L

R

R

I

L

P

P

I

L

N

N

E

I

E

S

G

E

S

N

E

R

T

N

S

T

G

A

Q

-

I

I

Q

A

E

H

Y

A

L

L

D

S

A

S

Y

Y

R

R

G

G

E

S

G

G

I

V

Q

H

H
|
H

I

I

A

A

L

F

G

D

S

C

N

D

D

D

I

I

Y

F

R

R

T

E

V

V

D

A

G

R

L

A

R

K

A

L

A

A

N

G

I

V

S

P

L

L

L

L

D

E

T

I

I

P

D

L

T

N

Y

Y

Y

Y

E

D

L

D

V

L

D

A

R

A

R

R

V

F

P

D

N

F

H

D

G

D

E

E

P

F

L

L

D

S

E

E

L

L

R

A

K

Y

R

Y

K

N

I

V

L

L

I

Y

D

D

-

R

G

D

A

A

P

Q

D

G

D

G

L

E

L

L

L

F

Q

H

I

V

F

Y

T

T

E

E

N

P

Q

F

I

E

G

E

P

R

I

F

F

F

F

F

E
|
E

I

I

I

I

Q

Q

R

R

K

K

-

A

G

G

N

Y

Q

A

G

G

F

Y

G

G

E

A

G

A

N

N

H443 (= H173) binding Mg(2+)
H521 (= H252) binding Mg(2+)
E599 (= E329) binding Mg(2+)

Sites not aligning to the query:

134 binding a divalent metal cation
165 binding a divalent metal cation
168 H→A: 21-fold decrease in turnover rate.
191 binding a divalent metal cation
206 S→A: 10-fold decrease in the affinity for dehydroshikimate without significantly altering the turnover rate.
239 binding a divalent metal cation

1t47A Structure of fe2-hppd bound to ntbc (see paper)
39% identity, 77% coverage: 75:355/365 of query aligns to 81:360/362 of 1t47A

query
sites
1t47A

H

H

G

G

P

D

S

G

I

V

C

V

A

D

I

L

A

A

F

I

R

E

V

V

Q

P

D

D

A

A

G

R

K

A

A

A

Y

H

R

A

Q

Y

A

A

V

I

E

E

K

H

G

G

A

A

W

R

G

S

F

V

D

A

N

E

K

-

T

-

G

-

P

P

M

Y

E

E

L

L

N

K

-

D

-

E

-

H

-

G

-

T

-

V

-

V

I

L

P

A

A

A

I

I

K

A

G

T

I

Y

G

G

D

K

S

T

L

R

I

H

Y

T

F

L

V

V

D

D

R

R

W

T

R

G

G

Y

K

D

N

G

G

P

A

Y

A

L

P

P

G

G

S

Y

V

V

G

A

D

A

I

A

D

P

I

I

Y

-

D

-

V

-

D

-

F

V

E

E

P

P

I

-

P

-

G

-

A

-

N

-

P

-

N

-

P

P

V

A

G

H

H

R

G

T

L

F

T

Q

Y

A

I

I

D

D

H
|
H

L

C

T

V

H

G

N

N

V

V

H

E

R

L

G

G

R

R

M

M

Q

N

E

E

W

W

A

V

E

G

F

F

Y

Y

E

N

R

K

L

V

F

M

N

G

F

F

R

T

E

N

V

M

R

K

Y

E

F

F

-

V

-

G

-

D

D

D

I

I

E

A

G

T

K

E

V

Y

T

S

G

A

V

L

K

M

S
|
S

K

K

A

V

M

V

T

A

S

D

P

G

C

T

G

L

K

K

I

V

R

K

I

F

P
|
P

I

I

N

N

E

E

E

P

G

A

-

L

S

A

E

K

T

K

S

K

G

S

Q

Q

I

I

Q

D

E

E

Y

Y

L

L

D

E

A

F

Y

Y

R

G

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

L

G

N

S

T

N

G

D

D

I

I

Y

V

R

E

T

T

V

V

D

R

G

T

L

M

R

R

A

A

A

A

N

G

I

V

S

Q

L

F

L

L

D

D

T

T

I

P

D

D

T

S

Y

Y

Y

Y

E

D

L

T

V

L

D

G

R

E

R

W

V

V

P

G

N

D

H

T

G

R

E

V

P

P

L

V

D

D

E

T

L

L

R

R

K

E

R

L

K

K

I

I

L

L

I

A

D

D

G

R

A

D

P

E

D

D

D

G

L

Y

L

L

L

L

Q

Q

I

I

F
|
F

T

T

E

K

N

P

-

V

Q

Q

I

D

G

R

P

P

-

T

I

V

F

F

F

F

E
|
E

I

I

I

I

Q

E

R

R

K

H

G

G

N

S

Q

M

G

G

F
|
F

G
|
G

E

K

G

G

N
|
N

F
|
F

K

K

A

A

L
|
L

F

F

E

E

S

A

I

I

E

E

L

R

D

E

Q

Q

binding fe (ii) ion: H172 (= H173), H255 (= H252), E334 (= E329)
binding 2-{hydroxy[2-nitro-4-(trifluoromethyl)phenyl]methylene}cyclohexane-1,3-dione: H172 (= H173), S215 (= S213), P228 (= P226), H255 (= H252), F321 (= F318), E334 (= E329), F344 (= F339), G345 (= G340), N348 (= N343), F349 (= F344), L352 (= L347)

O52791 4-hydroxymandelate synthase; HMS; HmaS; 4-hydroxyphenylpyruvate dioxygenase II; EC 1.13.11.46 from Amycolatopsis orientalis (Nocardia orientalis) (see paper)
30% identity, 85% coverage: 45:355/365 of query aligns to 40:346/357 of O52791

query
sites
O52791

H

H

K

R

D

S

V

I

T

A

L

L

Y

-

R

R

Q

Q

G

G

D

Q

I

V

N

T

F

L

I

V

V

L

N

T

G

-

E

E

P

P

D

T

S

S

F

D

-

R

-

H

-

P

A

A

Q

A

R

A

F

Y

A

L

R

Q

L

T

H

H

G

G

P

D

S

G

I

V

C

A

A

D

I

I

A

A

F

M

R

A

V

T

Q

S

D

D

A

V

G

A

K

A

A

A

Y

Y

R

E

Q

A

A

A

V

V

E

R

K

A

G

G

A

A

W

E

G

A

F

V

D

R

-

A

-

P

N

G

K

Q

T

H

G

S

P

E

M

A

E

A

L

V

N

T

I

T

P

A

A

T

I

I

K

G

G

G

I

F

G

G

D

D

S

V

L

V

I

H

Y

T

F

L

V

I

D

Q

R

R

W

D

R

G

G

T

K

S

N

A

G

E

A

L

A

P

P

P

G

G

S

F

V

T

G

G

D

S

I

M

D

D

I

V

Y

T

-

N

-

H

-

G

-

K

-

G

D

D

V

V

D

D

F

-

E

-

P

-

I

-

P

-

G

-

A

-

N

-

P

-

N

-

P

-

V

-

G

-

H

-

G

-

L

L

T

L

Y

G

I

I

D

D

H

H

L

F

T

A

H

I

N

C

V

L

H

N

R

A

G

G

R

D

M

L

Q

G

E

P

W

T

A

V

E

E

F

Y

Y

Y

E

E

R

R

L

A

F

L

N

G

F

F

R

R

E

Q

V

I

R

F

Y

D

F

E

D

H

I

I

E

V

G

V

K

G

V

A

T

Q

G

A

V

M

K

N

S
|
S

K

T

A

V

M

V

T

Q

S

S

P

A

C

S

G

G

K

A

I

V

R

T

I

L

P
x
T

I

L

N

I

E

E

-

P

E

D

G

R

S

N

E

A

T

D

S

P

G

G

Q

Q

I

I

Q

D

E

E

Y

F

L

L

D

K

A

D

Y

H

R

Q

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

F

G

N

S

S

N

N

D

D

I

A

Y

V

R

R

T

A

V

V

D

K

G

A

L

L

R

S

A

E

A

R

N

G

I

V

S

E

L

F

L

L

D

K

T

T

I

P

D

G

T

A

Y

Y

Y

Y

E

D

L

L

V

L

D

G

R

E

R

R

V

I

P

T

N

L

H

Q

G

T

E

H

P

S

L

L

D

D

E

D

L

L

R

R

K

A

R

T

K

N

I

V

L

L

I

A

D

D

G

E

A

D

P

H

D

G

D

G

L

Q

L

L

L

F

Q
|
Q

I

I

F

F

T

T

E

A

N

S

Q

T

I

H

-

P

-

R

G

H

P

T

I

I

F

F

F

F

E

E

I

V

I

I

Q

E

R

R

K

Q

G

G

N

A

Q

G

G

T

F

F

G

G

E

S

G

S

N

N

F

I

K

K

A

A

L

L

F

Y

E

E

S

A

I

V

E

E

L

L

D

E

Q

R

S201 (= S213) binding substrate
T214 (≠ P226) binding substrate
H241 (= H252) binding substrate
Q305 (= Q316) binding substrate

2r5vA Hydroxymandelate synthase crystal structure (see paper)
30% identity, 85% coverage: 45:355/365 of query aligns to 39:344/346 of 2r5vA

query
sites
2r5vA

H

H

K

R

D

S

V

I

T

A

L

L

Y

-

R

R

Q

Q

G

G

D

Q

I

V

N

T

F

L

I

V

V

L

N

T

G

-

E

E

P

P

D

T

S

S

F

D

-

R

-

H

-

P

A

A

Q

A

R

A

F

Y

A

L

R

Q

L

T

H

H

G

G

P

D

S

G

I

V

C

A

A

D

I

I

A

A

F

M

R

A

V

T

Q

S

D

D

A

V

G

A

K

A

A

A

Y

Y

R

E

Q

A

A

A

V

V

E

R

K

A

G

G

A

A

W

E

G

A

F

V

D

R

N

A

K

P

-

G

T

Q

G

H

P

S

M

A

E

A

L

V

N

T

I

T

P

A

A

T

I

I

K

G

G

G

I

F

G

G

D

D

S

V

L

V

I

H

Y

T

F

L

V

I

D

Q

R

R

W

D

R

G

G

T

K

S

N

A

G

E

A

L

A

P

P

P

G

G

S

F

V

T

G

G

D

S

I

M

D

D

I

V

Y

T

-

N

-

H

-

G

-

K

-

G

D

D

V

V

D

D

F

-

E

-

P

-

I

-

P

-

G

-

A

-

N

-

P

-

N

-

P

-

V

-

G

-

H

-

G

-

L

L

T

L

Y

G

I

I

D

D

H
|
H

L

F

T

A

H

I

N

C

V

L

H

N

R

A

G

G

R

D

M

L

Q

G

E

P

W

T

A

V

E

E

F

Y

Y

Y

E

E

R

R

L

A

F

L

N

G

F

F

R

R

E

Q

V

I

R
x
F

Y

D

F

E

D

H

I

I

E

V

G

V

K

G

V

A

T

Q

G

A

V

M

K

N

S
|
S

K

T

A
x
V

M

V

T

Q

S

S

P

A

C

S

G

G

K

A

I

V

R

T

I

L

P
x
T

I

L

N

I

E

E

-

P

E

D

G

R

S

N

E

A

T

D

S

P

G

G

Q

Q

I

I

Q

D

E

E

Y

F

L

L

D

K

A

D

Y

H

R

Q

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

F

G

N

S

S

N

N

D

D

I

A

Y

V

R

R

T

A

V

V

D

K

G

A

L

L

R

S

A

E

A

R

N

G

I

V

S

E

L

F

L

L

D

K

T

T

I

P

D

G

T

A

Y

Y

Y

Y

E

D

L

L

V

L

D

G

R

E

R

R

V

I

P

T

N

L

H

Q

G

T

E

H

P

S

L

L

D

D

E

D

L

L

R

R

K

A

R

T

K

N

I

V

L

L

I

A

D

D

G

E

A

D

P

H

D

G

D

G

L

Q

L

L

L

F

Q
|
Q

I

I

F

F

T

T

E

A

N

S

Q

T

I

H

-

P

-

R

G

H

P

T

I

I

F

F

F

F

E
|
E

I

V

I

I

Q

E

R

R

K

Q

G

G

N

A

Q

G

G

T

F
|
F

G

G

E

S

G

S

N

N

F
x
I

K

K

A

A

L

L

F

Y

E

E

S

A

I

V

E

E

L

L

D

E

Q

R

binding cobalt (ii) ion: H159 (= H173), H239 (= H252), E318 (= E329)
binding (2S)-hydroxy(4-hydroxyphenyl)ethanoic acid: H159 (= H173), F186 (≠ R200), S199 (= S213), V201 (≠ A215), T212 (≠ P226), H239 (= H252), Q303 (= Q316), E318 (= E329), F328 (= F339), I333 (≠ F344)

8jbwA Crystal structure of zthppd-(+)-usnic acid complex (see paper)
32% identity, 78% coverage: 75:358/365 of query aligns to 92:364/364 of 8jbwA

query
sites
8jbwA

H

H

G

G

P

D

S

G

I

V

C

K

A

D

I

V

A

A

F

F

R

E

V

V

Q

D

D

D

A

V

G

L

K

A

A

V

Y

Y

R

E

Q

N

A

A

V

V

E

A

K

N

G

G

A

A

W

E

G

S

F

V

D

S

-

S

-

P

-

H

-

T

N

D

K

S

T

C

G

D

P

E

M

G

E

D

L

V

N

I

I

S

P

A

A

A

I

I

K

K

G

T

I

Y

G

G

D

D

S

T

L

T

I

H

Y

T

F

F

V

I

D

Q

R

R

W

T

R

T

G

Y

K

T

N

G

G

P

A

F

A

L

P

P

G

G

-

Y

-

R

S

S

V

C

G

T

D

T

I

V

D

D

I

S

Y

A

D

N

V

K

D

F

F

L

E

P

P

P

I

V

P

-

G

-

A

-

N

-

P

-

N

-

P

-

V

-

G

-

H

-

G

N

L

L

T

E

Y

A

I

I

D

D

H
|
H

L

C

T

V

H

G

N

N

V

Q

H

D

R

W

G

D

R

E

M
|
M

Q

S

E

D

W

A

A

C

E

D

F

F

Y

Y

E

E

R

R

L

C

F

L

N

G

F

F

R

H

E

-

V

-

R

R

Y

F

F

W

D

S

I

V

E

D

G

D

K

K

V

D

T
x
I

G

C

V

T

K

E

S
x
F

K

S

A

A

M

L

T

K

S

N

P

-

C

-

G

-

K

-

I

-

R

-

I

-

P

-

I

-

N

-

E

-

E

E

G

P

S

A

E

H

T

G

S

K

G

S

Q

Q

I

I

Q

E

E

E

Y

Y

L

V

D

D

A

F

Y

Y

R

N

G

G

E

P

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

L

G

R

S

T

N

P

D

N

I

I

Y

I

R

E

T

A

V

V

D

S

G

N

L

L

R

R

A

S

A

R

N

G

I

V

S

E

L

F

L

I

D

S

T

V

I

P

D

D

T

T

Y

Y

Y

Y

E

E

L

N

V

M

D

R

R

L

R

R

V

L

P

K

N

A

H

A

G

G

E

M

P

K

L

L

D

E

E

E

-

S

-

F

-

D

-

I

L

I

R

Q

K

K

R

L

K

N

I

I

L

L

I

I

D

D

G

F

A

D

P

E

D

G

D

G

L

Y

L

L

L

L

Q
|
Q

I

L

F

F

T

T

E

K

N

P

Q

L

I

M

G

D

-

R

-

P

P

T

I

V

F

F

F

I

E
|
E

I

I

I

I

Q

Q

R

R

K

N

G

N

N

F

Q

D

G

G

F

F

G

G

E

A

G

G

N

N

F

F

K

K

A

S

L

L

F

F

E

E

S

A

I

I

E

E

L

R

D

E

Q

Q

I

D

R

L

R

R

binding (9bR)-2,6-diethanoyl-8,9b-dimethyl-3,7,9-tris(oxidanyl)dibenzofuran-1-one: M196 (= M183), I220 (≠ T209), F224 (≠ S213), H252 (= H252)
binding cobalt (ii) ion: H186 (= H173), H252 (= H252), Q320 (= Q316), E335 (= E329)

P32755 4-hydroxyphenylpyruvate dioxygenase; 4-hydroxyphenylpyruvic acid oxidase; 4HPPD; HPD; HPPDase; F Alloantigen; F protein; EC 1.13.11.27 from Rattus norvegicus (Rat) (see 2 papers)
30% identity, 85% coverage: 52:363/365 of query aligns to 63:383/393 of P32755

query
sites
P32755

R

K

Q

Q

G

G

D

K

I

I

N

V

F

F

I

V

V

L

N

C

G

S

E

A

P

L

D

N

S

P

F

W

A

N

Q

K

R

E

F

M

A

G

R

D

-

H

-

L

-

V

L

K

H

H

G

G

P

D

S

G

I

V

C

K

A

D

I

I

A

A

F

F

R

E

V

V

Q

E

D

D

A

C

G

E

K

H

A

I

Y

V

R

Q

Q

K

A

A

V

R

E

E

K

R

G

G

A

A

-

K

-

I

-

V

-

R

-

E

-

P

W

W

G

V

F

E

D

E

N

D

K

K

T

F

G

G

P

K

M

V

E

K

L

F

N

A

I

V

P

-

A

-

I

L

K

Q

G

T

I

Y

G

G

D

D

S

T

L

T

I

H

Y

T

F

L

V

V

D

E

R

K

W

I

R

N

G

Y

K

T

N

G

G

R

A

F

A

L

P

P

G

G

S

F

V

E

G

A

D

P

I

T

D

-

I

Y

Y

K

D

D

V

T

D

L

F

L

E

P

P

K

I

L

P

P

G

S

A

C

N

N

P

-

N

-

P

-

V

-

G

-

H

-

G

-

L

L

T

E

Y

I

I

I

D

D

H
|
H

L

I

T

V

H

G

N

N

V

Q

H

P

R

D

G

Q

R

E

M

M

Q

E

E

S

W

A

A

S

E

E

F

W

Y

Y

E

L

R

K

L

N

F

L

N

Q

F

F

R

H

E

R

V

F

R

W

Y

S

F

V

D

D

-

D

-

T

-

Q

I

V

E

H

G

T

K

E

V

Y

T

S

G

S

V

L

K

R

S

S

K

I

A

V

M

V

T

A

S

N

P

Y

C

E

G

E

K

S

I

I

R

K

I

M

P

P

I

I

N

N

E

E

E

P

G

A

-

P

S

G

E

R

T

K

S

K

G

S

Q

Q

I

I

Q

Q

E

E

Y

Y

L

V

D

D

A

Y

Y

N

R

G

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

L

G

R

S

T

N

E

D

D

I

I

Y

I

R

T

T

T

V

I

D

R

G

H

L

L

R

R

A

E

A

R

N

G

I

M

S

E

L

F

L

L

D

A

T

V

I

P

D

S

T

S

Y

Y

Y

Y

E

R

L

L

V

L

D

R

R

E

R

N

V

L

P

K

N

T

H

S

-

K

-

I

-

Q

-

V

G

K

E

E

P

N

L

M

D

D

E

V

L

L

R

E

K

E

R

L

K

K

I

I

L

L

I

V

D

D

G

Y

A

D

P

E

D

K

D

G

L

Y

L

L

L

L

Q

Q

I

I

F

F

T

T

E

K

-

P

N

M

Q

Q

I

D

G

R

P

P

-

T

I

L

F

F

F

L

E
|
E

I

V

I

I

Q

Q

R

R

K

H

G

N

N

H

Q

Q

G

G

F

F

G

G

E

A

G

G

N

N

F

F

K

N

A

S

L

L

F

F

E

K

S

A

I

F

E

E

L

E

D

E

Q

Q

I

A

R

L

R

R

G

G

V

N

V

L

Q

T

D

D

H183 (= H173) binding Fe cation
H266 (= H252) binding Fe cation
E349 (= E329) binding Fe cation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylthreonine

7yvvA Acmp1, r-4-hydroxymandelate synthase
31% identity, 82% coverage: 55:352/365 of query aligns to 48:331/335 of 7yvvA

query
sites
7yvvA

D

D

I

I

N

R

F

F

I

V

V

F

N

S

G

-

E

E

P

P

-

L

-

V

-

D

D

D

S

H

F

P

A

G

Q

T

R

A

F

Y

A

V

R

D

L

R

H

H

G

G

P

D

S

G

I

V

C

S

A

D

I

I

A

A

F

L

R

R

V

V

Q

T

D

D

A

A

G

K

K

A

A

A

Y

F

R

E

Q

E

A

A

V

V

E

A

K

R

G

G

A

A

W

R

G

P

F

V

D

A

N

G

K

P

T

S

G

R

P

A

M

G

E

H

L

V

N

V

I

T

P

A

A

S

I

I

K

M

G

G

I

F

G

G

D

D

S

T

L

L

I

H

Y

T

F

F

V

V

D

E

R

Y

W

P

R

D

G

G

K

P

N

-

G

-

A

P

A

A

P

P

G

I

S

A

V

V

G

N

D

A

I

E

D

E

I

-

Y

-

D

-

V

-

D

-

F

-

E

-

P

-

I

-

P

P

G

G

A

A

N

-

P

-

N

-

P

-

V

-

G

-

H

-

G

-

L

L

T

L

Y

E

I

I

D

D

H
|
H

L

F

T

A

H

V

N

C

V

V

H

E

R

H

G

G

R

H

M

L

Q

D

E

A

W

T

A

V

E

D

F

F

Y

Y

E

R

R

D

L

V

F

L

N

G

F

F

R

E

E

L

V

I

R

F

Y

A

F

E

D

T

I

I

E

A

G

V

K

G

V

S

T

Q

G

A

V

M

K

T

S

T

K

K

A

V

M

V

T

Q

S

S

P

K

C

S

G

G

K

S

I

V

R

T

I

F

P

T

I

L

N

I

E

E

-

P

E

D

G

T

S

S

E

K

T

D

S

P

G

G

Q

H

I

I

Q

D

E

D

Y

F

L

L

D

K

A

D

Y

H

R

G

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

F

G

T

S

S

N

N

D

G

I

I

Y

I

R

E

T

A

V

V

D

D

G

V

L

L

R

R

A

D

A

R

N

G

I

V

S

E

L

F

L

M

D

G

T

T

I

P

D

A

T

S

Y

Y

Y

Y

-

A

E

D

L

L

V

L

D

Q

R

R

R

V

V

V

P

P

N

E

H

Q

G

-

E

Y

P

S

L

V

D

P

E

E

L

L

R

R

K

T

R

Q

K

Q

I

V

L

L

I

V

D

D

G

E

A

D

P

H

D

D

D

G

L

Q

L

L

L

Y

Q

Q

I

I

F

F

T

A

E

R

N

S

Q

-

I

V

G

H

P

P

-

R

-

N

-

T

I

I

F

F

F

L

E
|
E

I

L

I

I

Q

E

R

R

K

L

G

G

N

A

Q

R

G

G

F

F

G

G

E

S

G

G

N

N

F

I

K

T

A

A

L

L

F

Y

E

Q

S

A

I

A

E

E

binding fe (iii) ion: H149 (= H173), H229 (= H252), E308 (= E329)

5ec3A Structural insight into the catalyitc mechanism of human 4- hydroxyphenylpyruvate dioxygenase
29% identity, 84% coverage: 52:359/365 of query aligns to 55:371/376 of 5ec3A

query
sites
5ec3A

R

K

Q

Q

G

G

D

K

I

I

N

V

F

F

I

V

V

L

N

S

G

S

E

A

P

L

D

N

S

P

F

W

A

N

Q

K

R

E

F

M

A

G

R

D

-

H

-

L

-

V

L

K

H

H

G

G

P

D

S

G

I

V

C

K

A

D

I

I

A

A

F

F

R

E

V

V

Q

E

D

D

A

C

G

D

K

Y

A

I

Y

V

R

Q

Q

K

A

A

V

R

E

E

K

R

G

G

A

A

-

K

-

I

-

M

-

R

-

E

-

P

W

W

G

V

F

E

D

Q

N

D

K

K

T

F

G

G

P

K

M

V

E

K

L

F

N

A

I

V

P

-

A

-

I

L

K

Q

G

T

I

Y

G

G

D

D

S

T

L

T

I

H

Y

T

F

L

V

V

D

E

R

K

W

M

R

N

G

Y

K

I

N

G

G

Q

A

F

A

L

P

P

G

G

S

Y

V

E

G

A

D

P

I

A

D

-

I

F

Y

M

D

D

V

P

D

L

F

L

E

P

P

K

I

L

P

P

G

K

A

C

N

S

P

-

N

-

P

-

V

-

G

-

H

-

G

-

L

L

T

E

Y

M

I

I

D

D

H
|
H

L

I

T

V

H

G

N

N

V

Q

H

P

R

D

G

Q

R

E

M

M

Q

V

E

S

W

A

A

S

E

E

F

W

Y

Y

E

L

R

K

L

N

F

L

N

Q

F

F

R

H

E

R

V

F

R

W

Y

S

F

V

D

D

-

D

-

T

-

Q

I

V

E

H

G

T

K

E

V

Y

T

S

G

S

V

L

K

R

S

S

K

I

A

V

M

V

T

A

S

N

P

Y

C

E

G

E

K

S

I

I

R

K

I

M

P

P

I

I

N

N

E

E

E

P

G

A

-

P

S

G

E

K

T

K

S

K

G

S

Q

Q

I

I

Q

Q

E

E

Y

Y

L

V

D

D

A

Y

Y

N

R

G

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

L

G

K

S

T

N

E

D

D

I

I

Y

I

R

T

T

A

V

I

D

R

G

H

L

L

R

R

A

E

A

R

N

G

I

L

S

E

L

F

L

L

D

S

T

V

I

P

D

S

T

T

Y

Y

Y

Y

E

K

L

Q

V

L

D

R

R

E

R

K

V

L

P

K

N

T

H

A

-

K

-

I

-

K

-

V

G

K

E

E

P

N

L

I

D

D

E

A

L

L

R

E

K

E

R

L

K

K

I

I

L

L

I

V

D

D

G

Y

A

D

P

E

D

K

D

G

L

Y

L

L

L

L

Q

Q

I

I

F

F

T

T

E

K

N

P

-

V

Q

Q

I

D

G

R

P

P

-

T

I

L

F

F

F

L

E
|
E

I

V

I

I

Q

Q

R

R

K

H

G

N

N

H

Q

Q

G

G

F

F

G

G

E

A

G

G

N

N

F

F

K

N

A

S

L

L

F

F

E

K

S

A

I

F

E

E

L

E

D

E

Q

Q

I

N

R

L

R

R

G

G

binding cobalt (ii) ion: H175 (= H173), H258 (= H252), E341 (= E329)

8im2A Crystal structure of human hppd complexed with ntbc (see paper)
29% identity, 84% coverage: 52:359/365 of query aligns to 57:373/374 of 8im2A

query
sites
8im2A

R

K

Q

Q

G

G

D

K

I

I

N

V

F

F

I

V

V

L

N

S

G

S

E

A

P

L

D

N

S

P

F

W

A

N

Q

K

R

E

F

M

A

G

R

D

-

H

-

L

-

V

L

K

H

H

G

G

P

D

S

G

I

V

C

K

A

D

I

I

A

A

F

F

R

E

V

V

Q

E

D

D

A

C

G

D

K

Y

A

I

Y

V

R

Q

Q

K

A

A

V

R

E

E

K

R

G

G

A

A

-

K

-

I

-

M

-

R

-

E

-

P

W

W

G

V

F

E

D

Q

N

D

K

K

T

F

G

G

P

K

M

V

E

K

L

F

N

A

I

V

P

-

A

-

I

L

K

Q

G

T

I

Y

G

G

D

D

S

T

L

T

I

H

Y

T

F

L

V

V

D

E

R

K

W

M

R

N

G

Y

K

I

N

G

G

Q

A

F

A

L

P

P

G

G

S

Y

V

E

G

A

D

P

I

A

D

-

I

F

Y

M

D

D

V

P

D

L

F

L

E

P

P

K

I

L

P

P

G

K

A

C

N

S

P

-

N

-

P

-

V

-

G

-

H

-

G

-

L

L

T

E

Y

M

I

I

D

D

H
|
H

L

I

T

V

H

G

N

N

V

Q

H

P

R

D

G

Q

R

E

M

M

Q

V

E

S

W

A

A

S

E

E

F

W

Y

Y

E

L

R

K

L

N

F

L

N

Q

F

F

R

H

E

R

V

F

R

W

Y

S

F

V

D

D

-

D

-

T

-

Q

I

V

E

H

G

T

K

E

V

Y

T

S

G

S

V

L

K

R

S

S

K

I

A

V

M

V

T

A

S

N

P

Y

C

E

G

E

K

S

I

I

R

K

I

M

P
|
P

I

I

N
|
N

E

E

E

P

G

A

-

P

S

G

E

K

T

K

S

K

G

S

Q

Q

I

I

Q

Q

E

E

Y

Y

L

V

D

D

A

Y

Y

N

R

G

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

L

G

K

S

T

N

E

D

D

I

I

Y

I

R

T

T

A

V

I

D

R

G

H

L

L

R

R

A

E

A

R

N

G

I

L

S

E

L

F

L

L

D

S

T

V

I

P

D

S

T

T

Y

Y

Y

Y

E

K

L

Q

V

L

D

R

R

E

R

K

V

L

P

K

N

T

H

A

-

K

-

I

-

K

-

V

G

K

E

E

P

N

L

I

D

D

E

A

L

L

R

E

K

E

R

L

K

K

I

I

L

L

I

V

D

D

G

Y

A

D

P

E

D

K

D

G

L

Y

L

L

L

L

Q

Q

I

I

F
|
F

T

T

E

K

N

P

-

V

Q

Q

I

D

G

R

P

P

-

T

I

L

F
|
F

F

L

E
|
E

I

V

I

I

Q

Q

R

R

K

H

G

N

N

H

Q

Q

G

G

F
|
F

G

G

E

A

G

G

N
|
N

F
|
F

K

N

A

S

L
|
L

F

F

E

K

S

A

I

F

E

E

L

E

D

E

Q

Q

I

N

R

L

R

R

G

G

binding cobalt (ii) ion: H177 (= H173), H260 (= H252), E343 (= E329)
binding 2-{hydroxy[2-nitro-4-(trifluoromethyl)phenyl]methylene}cyclohexane-1,3-dione: P233 (= P226), N235 (= N228), H260 (= H252), F330 (= F318), F341 (= F327), F353 (= F339), N357 (= N343), F358 (= F344), L361 (= L347)

3zgjB S221m v223f y359a mutant of 4-hydroxymandelate synthase from streptomyces coelicolor (see paper)
28% identity, 91% coverage: 20:352/365 of query aligns to 12:339/343 of 3zgjB

query
sites
3zgjB

Y

Y

T

V

A

A

P

D

D

D

P

R

K

E

A

A

L

S