SitesBLAST

Q94JQ4 Reactive Intermediate Deaminase A, chloroplastic; 2-iminobutanoate/2-iminopropanoate deaminase; EC 3.5.99.10 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 98% coverage: 3:130/130 of query aligns to 64:187/187 of Q94JQ4

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R165 (= R108) mutation to A: Loss of activity.

A0A1J1DL12 2-iminobutanoate/2-iminopropanoate deaminase; Allergen Der f 34; Enamine/imine deaminase; Allergen Der f 34.0101; EC 3.5.99.10 from Dermatophagoides farinae (American house dust mite) (see paper)
31% identity, 85% coverage: 20:130/130 of query aligns to 17:127/128 of A0A1J1DL12

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Y19 (≠ T22) mutation to A: Loss of imine deaminase activity; when associated with A-33; A-59; A-91; A-105; A-114 and A-120.
G33 (= G36) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-59; A-91; A-105; A-114 and A-120.
N59 (≠ Q62) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-91; A-105; A-114 and A-120.
N91 (= N94) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-105; A-114 and A-120.
R105 (= R108) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-114 and A-120.
P114 (= P117) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-120.
E120 (= E123) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-114.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5hp8A Crystal structures of rida in complex with pyruvate (see paper)
32% identity, 83% coverage: 23:130/130 of query aligns to 1:108/108 of 5hp8A

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binding pyruvic acid: R86 (= R108), T88 (≠ F110)

P52759 2-iminobutanoate/2-iminopropanoate deaminase; Liver perchloric acid-soluble protein; L-PSP; Reactive intermediate imine deaminase A homolog; Translation inhibitor L-PSP ribonuclease; UK114 antigen homolog; rp14.5; EC 3.5.99.10 from Rattus norvegicus (Rat) (see paper)
33% identity, 75% coverage: 31:128/130 of query aligns to 30:127/137 of P52759

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

3vczB 1.80 angstrom resolution crystal structure of a putative translation initiation inhibitor from vibrio vulnificus cmcp6
37% identity, 77% coverage: 29:128/130 of query aligns to 23:125/127 of 3vczB

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binding magnesium ion: D79 (≠ S86), N81 (≠ E88)

2uynA Crystal structure of e. Coli tdcf with bound 2-ketobutyrate (see paper)
32% identity, 88% coverage: 15:128/130 of query aligns to 4:124/127 of 2uynA

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binding 2-ketobutyric acid: F83 (= F90), R104 (= R108), S105 (≠ I109), C106 (≠ F110)

2uykC Crystal structure of e. Coli tdcf with bound serine (see paper)
32% identity, 88% coverage: 15:128/130 of query aligns to 4:124/127 of 2uykC

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binding serine: R104 (= R108), S105 (≠ I109), C106 (≠ F110)

Q7CP78 2-iminobutanoate/2-iminopropanoate deaminase; Enamine/imine deaminase; EC 3.5.99.10 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
35% identity, 77% coverage: 29:128/130 of query aligns to 24:125/128 of Q7CP78

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R105 (= R108) mutation to A: Deaminase activity is defective. At pH 8 and 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
E120 (= E123) Important for catalytic activity at high pH; mutation to A: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.; mutation to K: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.

Sites not aligning to the query:

17 Y→F: Deaminase activity is defective. At pH 8, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.

P80601 2-iminobutanoate/2-iminopropanoate deaminase; 14.3 kDa perchloric acid soluble protein; Translation inhibitor L-PSP ribonuclease; UK114 antigen; EC 3.5.99.10; EC 3.1.-.- from Capra hircus (Goat) (see paper)
28% identity, 83% coverage: 21:128/130 of query aligns to 20:127/137 of P80601

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

P0AFQ5 3-aminoacrylate deaminase RutC; 3-AA deaminase; EC 3.5.-.- from Escherichia coli (strain K12) (see paper)
35% identity, 68% coverage: 20:108/130 of query aligns to 16:104/128 of P0AFQ5

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F

C

N

N

S

V

I

Y

F

C

I

T

T

S

D

V

W

E

K

M

N

F

Y

P

A

I

A

V

I

N

N

E

E

I

I

Y

Y

A

A

E

E

F

F

G

P

P

G

Q

D

P

K

P

P

A

A

R
|
R

R104 (= R108) mutation to A: Loss of activity.

3lybB Structure of putative endoribonuclease(kp1_3112) from klebsiella pneumoniae
25% identity, 67% coverage: 27:113/130 of query aligns to 6:117/137 of 3lybB

query
sites
3lybB

R

R

H

A

G

G

D

D

T

F

L

I

Y

F

V

L

S

S

G

G

L

I

P

I

P

P

F

V

D

N

P

P

Q

L

T

T

G

G

E

T

V

I

L

V

D
x
N

A

G

-

F

-

Q

-
x
D

-

V

-

P

-

E

-

P

-

V

-

R

-

E

-

L

-

G

-

A

-

T

-

G

-

E

-

F

-

S

-

T

-

D

-

A

-

K

-

Q

-

G

P

P

I

I

E

L

Q

A

Q

Q

T

S

R

W

R

Y

V

V

L

L

E

E

Q

S

L

I

K

R

L

R

C

T

V

V

E

A

T

S

G

A

G

G

S

G

S

Q

L

M

D

S

Q

D

V

V

L

I

K

K

C

L

N

V

V

Q

Y

Y

C

F

T

R

S

N

V

L

E

D

M

H

F

F

P

P

I

Y

V

Y

N

S

E

R

I

V

Y

R

A

K

E

L

F

F

F

Y

G

P

P

D

Q

Q

P

P

A

V

R

S

I

T

F

V

V

V

N

Q

V

V

binding calcium ion: N28 (≠ D49), D32 (vs. gap)

Query Sequence

>H281DRAFT_05869 FitnessBrowser__Burk376:H281DRAFT_05869
MAREIVRVEPLSSWLERWKAPTSVATRHGDTLYVSGLPPFDPQTGEVLDAPIEQQTRRVL
EQLKLCVETGGSSLDQVLKCNVYCTSVEMFPIVNEIYAEFFGPQPPARIFVNVPAWPGRF
DIEIDCIAAV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory