Comparing H281DRAFT_05885 FitnessBrowser__Burk376:H281DRAFT_05885 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 9 hits to proteins with known functional sites (download)
P76015 PEP-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121 from Escherichia coli (strain K12) (see 3 papers)
48% identity, 100% coverage: 1:328/329 of query aligns to 1:355/356 of P76015
Q9CIV8 PTS-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see paper)
47% identity, 99% coverage: 2:328/329 of query aligns to 4:332/332 of Q9CIV8
3ct4A Structure of dha-kinase subunit dhak from l. Lactis (see paper)
47% identity, 99% coverage: 2:327/329 of query aligns to 1:318/318 of 3ct4A
1uodA Crystal structure of the dihydroxyacetone kinase from e. Coli in complex with dihydroxyacetone-phosphate (see paper)
46% identity, 97% coverage: 10:328/329 of query aligns to 1:335/336 of 1uodA
1uoeA Crystal structure of the dihydroxyacetone kinase from e. Coli in complex with glyceraldehyde (see paper)
46% identity, 96% coverage: 12:328/329 of query aligns to 3:335/336 of 1uoeA
3pnqD Crystal structure of e.Coli dha kinase dhak (h56n) complex with dha (see paper)
45% identity, 97% coverage: 10:328/329 of query aligns to 1:333/334 of 3pnqD
Q3LXA3 Triokinase/FMN cyclase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); EC 2.7.1.28; EC 2.7.1.29; EC 4.6.1.15 from Homo sapiens (Human) (see 4 papers)
41% identity, 99% coverage: 2:327/329 of query aligns to 4:336/575 of Q3LXA3
Sites not aligning to the query:
P45510 Dihydroxyacetone kinase; DHA kinase; Glycerone kinase; EC 2.7.1.29 from Citrobacter freundii (see 2 papers)
34% identity, 97% coverage: 4:322/329 of query aligns to 5:324/552 of P45510
Sites not aligning to the query:
1un9A Crystal structure of the dihydroxyacetone kinase from c. Freundii in complex with amp-pnp and mg2+ (see paper)
34% identity, 97% coverage: 4:322/329 of query aligns to 5:324/537 of 1un9A
Sites not aligning to the query:
>H281DRAFT_05885 FitnessBrowser__Burk376:H281DRAFT_05885
MKKFINHVDDFLAESLAGFAAAHGDLVVLNREPVFVRRKDLKPGKVALISGGGSGHEPLH
SGFVGHGMLDAACPGQIFTSPTPDQMMAAAKAVDTGAGVLFIVKNYSGDLMNFEMASEMS
EVPNAMVLINDDVAVENSSYTTGRRGVAGAVIVEKLVGSLAESGASLDECKAFGNRINKR
TASMGVAFSSCTVPAAGTLTFKIGDDEIEVGVGIHGEPGRRRAKFASADTIAAELLTAIV
DDLKPAAGAGVLVLVNGLGGTPLGELYLLFNSTRAWLEKRDLKIARVQVGSLTTSLEMAG
ASITLCMLDEQMTKHWDSAVHTPALRWGV
Or try a new SitesBLAST search
SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory