SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_05890 FitnessBrowser__Burk376:H281DRAFT_05890 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

8hpsB ABC transporter, permease protein SugB (see paper)
37% identity, 61% coverage: 114:307/317 of query aligns to 67:263/264 of 8hpsB

query
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8hpsB

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binding alpha-D-glucopyranose: H113 (≠ P160), F200 (= F247)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
30% identity, 67% coverage: 105:316/317 of query aligns to 78:295/296 of P68183

query
sites
P68183

P

P

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K

E190 (= E212) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A214) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G218) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P231) mutation to A: No effect.

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
23% identity, 67% coverage: 104:316/317 of query aligns to 69:280/281 of P94530

query
sites
P94530

G

G

P

G

S

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K

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K

D180 (= D217) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

Query Sequence

>H281DRAFT_05890 FitnessBrowser__Burk376:H281DRAFT_05890
MRPTTAQHSVVASSPRGKRFAAAIVITYALIAILPILWICLTSFKTQEDAIAYPPVVLFQ
PSMEGYVNLFTIRSRQTPEFIASLPPAQTWYERDVRKRNMVIAGPSKVLPRFVNSLVIGF
GSTFLAVFLGTLAAYAFSRFKVPLADDLLFFILSTRMMPPIAVAIPIYLMYRALGLSDSY
VGMIVLYTAVNVSLAVWLLKGFMDEIPREYEEAALVDGYTRLQAFVKVVLPQAITGIAAT
AIFCLIFAWNEYAFASLLTSGDAQTMPPFIPFIIGEGGQDWPAVAAATTLFVVPILIFTV
VLRKHLLRGITFGAVRK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory