SitesBLAST
Comparing H281DRAFT_05933 FitnessBrowser__Burk376:H281DRAFT_05933 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3ihbA Crystal structure analysis of mglu in its tris and glutamate form (see paper)
42% identity, 91% coverage: 21:296/304 of query aligns to 22:299/450 of 3ihbA
- active site: S64 (= S63), K67 (= K66), Y191 (= Y188), Y243 (= Y240), V261 (= V258)
- binding glutamic acid: Q63 (= Q62), S64 (= S63), N114 (= N113), E160 (= E157), N167 (= N164), G260 (= G257), V261 (= V258)
5w2jB Crystal structure of dimeric form of mouse glutaminasE C (see paper)
36% identity, 95% coverage: 1:289/304 of query aligns to 90:380/411 of 5w2jB
Sites not aligning to the query:
P77454 Glutaminase 1; EC 3.5.1.2 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 94% coverage: 18:304/304 of query aligns to 21:308/310 of P77454
- K69 (= K66) mutation to A: Loss of activity.
- N117 (= N113) mutation to A: Loss of activity.
- S160 (= S156) mutation to A: Loss of activity.
- E161 (= E157) mutation to A: Strongly reduced activity.
- Q162 (≠ L158) mutation to A: No effect.
- N168 (= N164) mutation to A: Loss of activity.
- Y192 (= Y188) mutation to A: Loss of activity.
- Y244 (= Y240) mutation to A: Loss of activity.
- S260 (= S256) mutation to A: Reduced activity.
- K294 (≠ L290) modified: N6-acetyllysine
D3Z7P3 Glutaminase kidney isoform, mitochondrial; GLS; EC 3.5.1.2 from Mus musculus (Mouse) (see 3 papers)
36% identity, 95% coverage: 1:289/304 of query aligns to 230:520/674 of D3Z7P3
- Y254 (= Y26) mutation to F: Increased enzyme activity in the absence of phosphate. No effect on stimulation of enzyme activity by phosphate.
- S291 (= S63) binding
- K316 (≠ R87) mutation to Q: Forms dimers with full, phosphate-independent activity; when associated with A-325 and K-391.
- G320 (= G91) mutation to P: Loss of enzyme activity.
- 320:327 (vs. 91:98, 50% identical) Highly mobile activation loop
- K325 (≠ S96) mutation to A: Constitutive enzyme activity that is fully active also in the absence phosphate. Forms oligomers with full, phosphate-independent activity; when associated with K-391. Forms dimers with full, phosphate-independent activity; when associated with Q-316 and K-391.
- N340 (= N113) binding
- E386 (= E157) binding
- D391 (≠ H162) mutation to K: Abolishes assembly of dimers into functional tetramers. Loss of enzyme activity. Forms oligomers with full, phosphate-independent activity; when associated with A-325. Forms dimers with full, phosphate-independent activity; when associated with Q-316 and A-325.
- N393 (= N164) binding
- F394 (≠ R165) mutation to S: Impairs tetramerization and promotes formation of homodimers. Impairs activation by phosphate.
- Y419 (= Y188) binding
- Y471 (= Y240) binding
- V489 (= V258) binding
Sites not aligning to the query:
- 202 K→E: Increased stimulation of enzyme activity by phosphate.
- 207 K→E: Increased stimulation of enzyme activity by phosphate.
P13264 Glutaminase kidney isoform, mitochondrial; GLS; K-glutaminase; L-glutamine amidohydrolase; EC 3.5.1.2 from Rattus norvegicus (Rat) (see paper)
36% identity, 95% coverage: 1:289/304 of query aligns to 230:520/674 of P13264
Sites not aligning to the query:
4o7dA Crystal structure of human glutaminase in complex don (see paper)
36% identity, 95% coverage: 1:289/304 of query aligns to 7:297/313 of 4o7dA
- active site: S68 (= S63), K71 (= K66), Y196 (= Y188), Y248 (= Y240), V266 (= V258)
- binding 5-oxo-l-norleucine: Y31 (= Y26), Q67 (= Q62), S68 (= S63), N117 (= N113), E163 (= E157), Y196 (= Y188), Y248 (= Y240), G265 (= G257), V266 (= V258)
6umfA Crystal structure of human gac in complex with inhibitor upgl00012
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/409 of 6umfA
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding N-(5-{[1-(5-amino-1,3,4-thiadiazol-2-yl)piperidin-4-yl]oxy}-1,3,4-thiadiazol-2-yl)-2-phenylacetamide: L185 (= L97), L187 (≠ Q99), Y258 (≠ F170)
6uljA Crystal structure of human gac in complex with inhibitor upgl00012
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/409 of 6uljA
6ulaA Crystal structure of human gac in complex with inhibitor upgl00012
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/409 of 6ulaA
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding 2-cyclopropyl-N-{5-[4-({5-[(cyclopropylacetyl)amino]-1,3,4-thiadiazol-2-yl}oxy)piperidin-1-yl]-1,3,4-thiadiazol-2-yl}acetamide: K184 (≠ S96), L185 (= L97), F186 (≠ V98), L187 (≠ Q99), N188 (≠ L100), Y258 (≠ F170)
6ukbA Crystal structure of human gac in complex with inhibitor upgl00012
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/409 of 6ukbA
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding N-[5-(4-{[5-(acetylamino)-1,3,4-thiadiazol-2-yl]oxy}piperidin-1-yl)-1,3,4-thiadiazol-2-yl]acetamide: K184 (≠ S96), L185 (= L97), F186 (≠ V98), L187 (≠ Q99), Y258 (≠ F170)
6ujmA Crystal structure of human gac in complex with inhibitor upgl00013
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/409 of 6ujmA
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding N-{5-[(3R)-3-{[5-(acetylamino)-1,3,4-thiadiazol-2-yl]amino}pyrrolidin-1-yl]-1,3,4-thiadiazol-2-yl}acetamide: K184 (≠ S96), L185 (= L97), F186 (≠ V98), L187 (≠ Q99), Y258 (≠ F170)
6umcB Crystal structure of human gac in complex with inhibitor upgl00012
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/410 of 6umcB
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding 2-phenyl-N-{5-[(3R)-3-({5-[(phenylacetyl)amino]-1,3,4-thiadiazol-2-yl}oxy)pyrrolidin-1-yl]-1,3,4-thiadiazol-2-yl}acetamide: K184 (≠ S96), L185 (= L97), F186 (≠ V98), L187 (≠ Q99), E189 (= E101), Y258 (≠ F170)
6ujgA Crystal structure of human gac in complex with inhibitor upgl00012
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/410 of 6ujgA
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding N-{5-[(3S)-3-{[5-(acetylamino)-1,3,4-thiadiazol-2-yl]amino}pyrrolidin-1-yl]-1,3,4-thiadiazol-2-yl}acetamide: K184 (≠ S96), L185 (= L97), F186 (≠ V98), L187 (≠ Q99), Y258 (≠ F170)
5wj6A Crystal structure of glutaminasE C in complex with inhibitor 2-phenyl- n-{5-[4-({5-[(phenylacetyl)amino]-1,3,4-thiadiazol-2-yl}amino) piperidin-1-yl]-1,3,4-thiadiazol-2-yl}acetamide (upgl-00004) (see paper)
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/410 of 5wj6A
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding 2-phenyl-N-{5-[4-({5-[(phenylacetyl)amino]-1,3,4-thiadiazol-2-yl}amino)piperidin-1-yl]-1,3,4-thiadiazol-2-yl}acetamide: R181 (≠ A93), K184 (≠ S96), L185 (= L97), F186 (≠ V98), L187 (≠ Q99), N188 (≠ L100), E189 (= E101), Y258 (≠ F170)
5i94A Crystal structure of human glutaminasE C in complex with the inhibitor upgl-00019 (see paper)
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/410 of 5i94A
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding 2-phenyl-N-{5-[4-({5-[(phenylacetyl)amino]-1,3,4-thiadiazol-2-yl}oxy)piperidin-1-yl]-1,3,4-thiadiazol-2-yl}acetamide: R181 (≠ A93), K184 (≠ S96), L185 (= L97), F186 (≠ V98), Y258 (≠ F170)
8bsnA Human gls in complex with compound 27 (see paper)
36% identity, 95% coverage: 1:289/304 of query aligns to 4:294/311 of 8bsnA
6umdB Crystal structure of human gac in complex with inhibitor upgl00012
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/409 of 6umdB
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding 2-(pyridin-3-yl)-N-(5-{4-[(5-{[(pyridin-3-yl)acetyl]amino}-1,3,4-thiadiazol-2-yl)amino]piperidin-1-yl}-1,3,4-thiadiazol-2-yl)acetamide: R181 (≠ A93), K184 (≠ S96), L185 (= L97), F186 (≠ L100), L187 (≠ E101), N188 (≠ A102), E189 (= E103), Y258 (≠ F170)
6ul9B Crystal structure of human gac in complex with inhibitor upgl00023
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/409 of 6ul9B
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding 2-phenyl-N-{5-[(1-{5-[(phenylacetyl)amino]-1,3,4-thiadiazol-2-yl}azetidin-3-yl)oxy]-1,3,4-thiadiazol-2-yl}acetamide: R181 (≠ A93), K184 (≠ S96), L185 (= L97), F186 (≠ L100), L187 (≠ E101), N188 (≠ A102), E189 (= E103), Y258 (≠ F170)
5fi7A Crystal structure of human gac in complex with inhibitor upgl_00015: 2-phenyl-~{n}-[5-[(3~{s})-3-[[5-(2-phenylethanoylamino)-1,3,4- thiadiazol-2-yl]oxy]pyrrolidin-1-yl]-1,3,4-thiadiazol-2-yl]ethanamide (see paper)
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/410 of 5fi7A
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding 2-phenyl-~{N}-[5-[(3~{S})-3-[[5-(2-phenylethanoylamino)-1,3,4-thiadiazol-2-yl]oxy]pyrrolidin-1-yl]-1,3,4-thiadiazol-2-yl]ethanamide: K184 (≠ S96), L185 (= L97), F186 (≠ V98), L187 (≠ Q99), E189 (= E101), Y258 (≠ F170)
5fi6A Crystal structure of human gac in complex with inhibitor upgl_00011: 2-phenyl-~{n}-[5-[[(3~{s})-1-[5-(2-phenylethanoylamino)-1,3,4- thiadiazol-2-yl]pyrrolidin-3-yl]amino]-1,3,4-thiadiazol-2- yl]ethanamide (see paper)
36% identity, 95% coverage: 1:289/304 of query aligns to 89:379/410 of 5fi6A
- active site: S150 (= S63), K153 (= K66), Y278 (= Y188), Y330 (= Y240), V348 (= V258)
- binding 2-phenyl-~{N}-[5-[[(3~{S})-1-[5-(2-phenylethanoylamino)-1,3,4-thiadiazol-2-yl]pyrrolidin-3-yl]amino]-1,3,4-thiadiazol-2-yl]ethanamide: R181 (≠ A93), F182 (= F94), K184 (≠ S96), L185 (= L97), F186 (≠ V98), L187 (≠ Q99), N188 (≠ L100), E189 (= E101), Y258 (≠ F170)
Query Sequence
>H281DRAFT_05933 FitnessBrowser__Burk376:H281DRAFT_05933
MNYASILEQIHRDLRPFLGTGKVADYIPQLATVPADSFGMAIVTPSGEVFTIGEADTRFS
IQSISKLFACTMAFRLLGDELWQRVGREPSGNAFNSLVQLEAEHGKPRNPFINAGALIVT
DVLCRRFVRAETALVEFMRRLTGEASIDYDLRVAQSELQHAHRNRAMAHFMASFGNMEMA
PEVVVDAYCRQCAISMSCVELAKAALFLTNHGVAPSTGERILDTSSAKRLSALMLTCGTY
DAAGDFVYRVGLPAKSGVGGGIVAVLPGEMAVCVWSPGLDANGNSLAGVLALEWLTTYTG
QSIF
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory