SitesBLAST
Comparing H281DRAFT_06296 FitnessBrowser__Burk376:H281DRAFT_06296 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
37% identity, 98% coverage: 4:255/258 of query aligns to 3:248/248 of 6ixmC
- active site: G16 (= G17), S142 (= S143), Y155 (= Y156), K159 (= K160)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S15 (≠ R16), G16 (= G17), I17 (= I18), D36 (≠ E37), I37 (≠ L38), A61 (≠ T63), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), M140 (≠ I141), S142 (= S143), Y155 (= Y156), K159 (= K160), P185 (= P186), A186 (≠ G187), Y187 (= Y188), I188 (= I189), L192 (= L193)
4urfB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
38% identity, 96% coverage: 4:251/258 of query aligns to 3:244/248 of 4urfB
- active site: G16 (= G17), S142 (= S143), I152 (≠ C153), Y155 (= Y156), K159 (= K160)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: L210 (≠ M217), R211 (≠ K218), R212 (= R219)
- binding bicarbonate ion: I92 (= I94), G94 (vs. gap), R109 (= R110), R179 (= R180), S228 (= S235)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), G14 (= G15), N15 (≠ R16), G16 (= G17), I17 (= I18), D36 (≠ E37), I37 (≠ L38), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (= I141), Y155 (= Y156), K159 (= K160), P185 (= P186), A186 (≠ G187), I188 (= I189), T190 (= T191)
4urfA Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
38% identity, 96% coverage: 4:251/258 of query aligns to 3:244/248 of 4urfA
- active site: G16 (= G17), S142 (= S143), I152 (≠ C153), Y155 (= Y156), K159 (= K160)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: I92 (= I94), S93 (≠ N95), G94 (vs. gap), E95 (≠ V96), T97 (≠ C98), E101 (≠ T102), T103 (= T104), Q106 (≠ D107), R109 (= R110), S175 (≠ P176), G177 (≠ N178)
- binding magnesium ion: S237 (≠ T244), Y238 (≠ C245)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), G14 (= G15), N15 (≠ R16), G16 (= G17), I17 (= I18), D36 (≠ E37), I37 (≠ L38), W41 (≠ T42), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (= I141), Y155 (= Y156), K159 (= K160), P185 (= P186), I188 (= I189), T190 (= T191)
4ureB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
38% identity, 96% coverage: 4:251/258 of query aligns to 3:244/248 of 4ureB
- active site: G16 (= G17), S142 (= S143), I152 (≠ C153), Y155 (= Y156), K159 (= K160)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: N15 (≠ R16), G16 (= G17), I17 (= I18), N89 (= N91), G91 (= G93), Y155 (= Y156), P185 (= P186), A186 (≠ G187)
4fn4A Short-chain NAD(h)-dependent dehydrogenase/reductase from sulfolobus acidocaldarius (see paper)
39% identity, 98% coverage: 4:255/258 of query aligns to 5:254/254 of 4fn4A
- active site: G18 (= G17), S144 (= S143), Y157 (= Y156), K161 (= K160), S202 (≠ D203)
- binding nicotinamide-adenine-dinucleotide: G14 (= G13), S17 (≠ R16), G18 (= G17), I19 (= I18), E38 (= E37), L39 (= L38), R43 (≠ T42), A63 (≠ T63), D64 (= D64), V65 (= V65), N91 (= N91), G93 (= G93), I94 (= I94), T142 (≠ I141), S144 (= S143), Y157 (= Y156), K161 (= K160), P187 (= P186), V190 (≠ I189), T192 (= T191), N193 (≠ Q192), I194 (≠ L193)
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
39% identity, 97% coverage: 3:251/258 of query aligns to 2:240/251 of 6xewA
- active site: G16 (= G17), S138 (= S143), Y151 (= Y156)
- binding r,3-hydroxybutan-2-one: S138 (= S143), S140 (≠ H145), Y151 (= Y156)
- binding s,3-hydroxybutan-2-one: S138 (= S143), Y151 (= Y156), S182 (≠ G187)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ R16), G16 (= G17), M17 (≠ I18), D36 (= D41), W37 (≠ T42), W37 (≠ T42), A38 (= A43), I59 (≠ T63), D60 (= D64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (= G93), V110 (= V114), T136 (≠ I141), S138 (= S143), Y151 (= Y156), K155 (= K160), S182 (≠ G187), L183 (≠ Y188), V184 (≠ I189), T186 (= T191), N187 (≠ Q192), M188 (≠ L193), T189 (= T194)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
39% identity, 97% coverage: 3:251/258 of query aligns to 2:240/251 of H9XP47
- N15 (≠ R16) binding
- M17 (≠ I18) binding
- D36 (= D41) binding
- D60 (= D64) binding
- V61 (= V65) binding
- N87 (= N91) binding
- S138 (= S143) binding ; binding
- V139 (≠ T144) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (≠ H145) binding
- Y151 (= Y156) binding ; binding ; binding
- K155 (= K160) binding
- V184 (≠ I189) binding
- T186 (= T191) binding
- RDK 197:199 (≠ PDP 202:204) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
39% identity, 97% coverage: 3:251/258 of query aligns to 4:242/252 of 6vspB
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
39% identity, 97% coverage: 3:251/258 of query aligns to 2:240/251 of 6vspA
- active site: G16 (= G17), S138 (= S143), Y151 (= Y156)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ R16), G16 (= G17), M17 (≠ I18), D36 (= D41), W37 (≠ T42), W37 (≠ T42), A38 (= A43), I59 (≠ T63), D60 (= D64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (= G93), V90 (≠ I94), V110 (= V114), T136 (≠ I141), S138 (= S143), Y151 (= Y156), K155 (= K160), P181 (= P186), S182 (≠ G187), L183 (≠ Y188), V184 (≠ I189), T186 (= T191), N187 (≠ Q192), M188 (≠ L193), T189 (= T194)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
37% identity, 97% coverage: 1:251/258 of query aligns to 2:241/244 of 4nbuB
- active site: G18 (= G17), N111 (≠ D115), S139 (= S143), Q149 (≠ C153), Y152 (= Y156), K156 (= K160)
- binding acetoacetyl-coenzyme a: D93 (≠ F97), K98 (≠ T102), S139 (= S143), N146 (≠ I150), V147 (≠ P151), Q149 (≠ C153), Y152 (= Y156), F184 (≠ Y188), M189 (≠ L193), K200 (≠ R208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ R16), G18 (= G17), I19 (= I18), D38 (≠ E37), F39 (≠ L38), V59 (≠ T63), D60 (= D64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (= G93), I90 (= I94), T137 (≠ I141), S139 (= S143), Y152 (= Y156), K156 (= K160), P182 (= P186), F184 (≠ Y188), T185 (≠ I189), T187 (= T191), M189 (≠ L193)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
36% identity, 99% coverage: 2:256/258 of query aligns to 9:267/267 of Q9LBG2
- 17:42 (vs. 10:35, 50% identical) binding
- E103 (≠ N95) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
35% identity, 98% coverage: 1:253/258 of query aligns to 2:253/255 of 5itvA
- active site: G18 (= G17), S141 (= S143), Y154 (= Y156), K158 (= K160)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), S17 (≠ R16), G18 (= G17), I19 (= I18), D38 (= D39), I39 (= I40), T61 (= T63), I63 (≠ V65), N89 (= N91), G91 (= G93), T139 (≠ I141), S141 (= S143), Y154 (= Y156), K158 (= K160), P184 (= P186), G185 (= G187), I186 (≠ Y188), I187 (= I189)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
37% identity, 97% coverage: 7:255/258 of query aligns to 6:246/248 of Q9KJF1
- S15 (≠ R16) binding
- D36 (≠ E37) binding
- D62 (= D64) binding
- I63 (≠ V65) binding
- N89 (= N91) binding
- Y153 (= Y156) binding
- K157 (= K160) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
37% identity, 95% coverage: 6:251/258 of query aligns to 5:240/244 of 6t77A
- active site: G16 (= G17), S138 (= S143), Y151 (= Y156)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ G15), R15 (= R16), T37 (= T42), L58 (≠ T63), N59 (≠ D64), V60 (= V65), A87 (= A92), G88 (= G93), I89 (= I94)
1iy8A Crystal structure of levodione reductase (see paper)
36% identity, 98% coverage: 3:256/258 of query aligns to 1:258/258 of 1iy8A
- active site: G15 (= G17), S143 (= S143), Q153 (≠ C153), Y156 (= Y156), K160 (= K160)
- binding nicotinamide-adenine-dinucleotide: G11 (= G13), S14 (≠ R16), G15 (= G17), L16 (≠ I18), D35 (≠ E37), V36 (≠ L38), A62 (≠ T63), D63 (= D64), V64 (= V65), N90 (= N91), G92 (= G93), I93 (= I94), T141 (≠ I141), S143 (= S143), Y156 (= Y156), K160 (= K160), P186 (= P186), G187 (= G187), T191 (= T191), P192 (≠ Q192), M193 (≠ L193)
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
37% identity, 97% coverage: 7:255/258 of query aligns to 5:245/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G13), M16 (≠ I18), D35 (≠ E37), I36 (≠ L38), I62 (≠ V65), N88 (= N91), G90 (= G93), I138 (= I141), S140 (= S143), Y152 (= Y156), K156 (= K160), I185 (= I189)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
37% identity, 98% coverage: 3:256/258 of query aligns to 7:254/258 of 4wecA
- active site: G21 (= G17), S143 (= S143), Q154 (vs. gap), Y157 (= Y156), K161 (= K160)
- binding nicotinamide-adenine-dinucleotide: G17 (= G13), A19 (≠ G15), S20 (≠ R16), G21 (= G17), I22 (= I18), D41 (≠ E37), I42 (≠ L38), V61 (≠ T63), D62 (= D64), V63 (= V65), N89 (= N91), T141 (≠ I141), Y157 (= Y156), K161 (= K160), P187 (= P186), P189 (≠ Y188), V190 (≠ I189)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
39% identity, 97% coverage: 3:251/258 of query aligns to 2:236/239 of 4nbtA
- active site: G16 (= G17), S132 (= S143), Y145 (= Y156), K149 (= K160)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), K15 (≠ R16), G16 (= G17), L17 (≠ I18), D36 (≠ E37), L37 (= L38), L52 (≠ T63), N53 (≠ D64), V54 (= V65), N80 (= N91), A81 (= A92), G82 (= G93), I130 (= I141), S132 (= S143), Y145 (= Y156), K149 (= K160), P177 (= P186), G178 (= G187), I180 (= I189), T182 (= T191)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
38% identity, 96% coverage: 4:251/258 of query aligns to 6:243/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), S17 (≠ G15), R18 (= R16), I20 (= I18), T40 (= T42), N62 (≠ D64), V63 (= V65), N89 (= N91), A90 (= A92), I92 (= I94), V139 (≠ I141), S141 (= S143), Y154 (= Y156), K158 (= K160), P184 (= P186), G185 (= G187), I187 (= I189), T189 (= T191), M191 (≠ L193)
2cfcA Structural basis for stereo selectivity in the (r)- and (s)-hydroxypropylethane thiosulfonate dehydrogenases (see paper)
37% identity, 97% coverage: 7:255/258 of query aligns to 3:250/250 of 2cfcA
- active site: G13 (= G17), S142 (= S143), Y155 (= Y156), K159 (= K160)
- binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ I150), R152 (≠ C153), Y155 (= Y156), W195 (= W197), R196 (≠ W198)
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), S12 (≠ R16), G13 (= G17), N14 (≠ I18), D33 (≠ E37), L34 (= L38), A59 (≠ T63), D60 (= D64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (= G93), I140 (= I141), P185 (= P186), G186 (= G187), M187 (≠ Y188), I188 (= I189), T190 (= T191), P191 (≠ Q192), M192 (≠ L193), T193 (= T194)
Query Sequence
>H281DRAFT_06296 FitnessBrowser__Burk376:H281DRAFT_06296
MTRLAGKVALVTGAGRGIGAAIALAFAREGAAVALAELDIDTARQTAQQISSETGARVLA
VHTDVTQSASVQHAVSEAEKKLGPLDVLVNNAGINVFCDPLTMTDDDWRRCFAVDLDGVW
NGCRAVLPGMVERGAGSILNIASTHAFKIIPGCFPYPVAKHGVIGLTRALGIEYAPRNVR
VNAIAPGYIETQLTHDWWNEQPDPAAARQATMDLQPMKRIGRPEEVAMTAVFLASDEAPF
INATCITVDGGRSVLYHD
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory