SitesBLAST
Comparing H281DRAFT_06377 FitnessBrowser__Burk376:H281DRAFT_06377 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7krmC Putative fabg bound to nadh from acinetobacter baumannii
66% identity, 100% coverage: 1:249/249 of query aligns to 1:244/244 of 7krmC
- active site: G18 (= G18), S140 (= S140), Y155 (= Y155)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), S15 (= S15), G18 (= G18), I19 (= I19), D38 (= D38), L39 (= L39), A60 (≠ C60), N61 (≠ D61), V62 (= V62), N88 (= N88), V111 (= V111), S140 (= S140), Y155 (= Y155), K159 (= K159), I188 (= I188), T190 (= T190)
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
41% identity, 100% coverage: 1:248/249 of query aligns to 1:243/244 of P0AEK2
- GAS--R 12:15 (≠ GAASPR 12:17) binding
- T37 (≠ L39) binding
- NV 59:60 (≠ DV 61:62) binding
- N86 (= N88) binding
- Y151 (= Y155) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YSAAK 155:159) binding
- A154 (= A158) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K159) mutation to A: Defect in the affinity for NADPH.
- I184 (= I188) binding
- E233 (≠ V238) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
41% identity, 99% coverage: 3:248/249 of query aligns to 2:242/243 of 1q7bA
- active site: G15 (= G18), E101 (≠ G104), S137 (= S140), Q147 (≠ G152), Y150 (= Y155), K154 (= K159)
- binding calcium ion: E232 (≠ V238), T233 (= T239)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (= R17), T36 (≠ L39), N58 (≠ D61), V59 (= V62), N85 (= N88), A86 (= A89), G87 (= G90), I88 (= I91), S137 (= S140), Y150 (= Y155), K154 (= K159), P180 (= P185), G181 (= G186), I183 (= I188)
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
40% identity, 99% coverage: 3:248/249 of query aligns to 2:242/243 of 1q7cA
- active site: G15 (= G18), S137 (= S140), Q147 (≠ G152), F150 (≠ Y155), K154 (= K159)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (= R17), A35 (≠ D38), T36 (≠ L39), L57 (≠ C60), N58 (≠ D61), V59 (= V62), G87 (= G90), I88 (= I91)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
41% identity, 100% coverage: 1:248/249 of query aligns to 4:246/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R17), I20 (= I19), T40 (≠ L39), N62 (≠ D61), V63 (= V62), N89 (= N88), A90 (= A89), I92 (= I91), V139 (≠ M138), S141 (= S140), Y154 (= Y155), K158 (= K159), P184 (= P185), G185 (= G186), I187 (= I188), T189 (= T190), M191 (≠ I192)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
39% identity, 100% coverage: 1:248/249 of query aligns to 1:243/244 of 6t77A
- active site: G16 (= G18), S138 (= S140), Y151 (= Y155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R17), T37 (≠ L39), L58 (≠ C60), N59 (≠ D61), V60 (= V62), A87 (= A89), G88 (= G90), I89 (= I91)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
38% identity, 99% coverage: 3:248/249 of query aligns to 5:244/244 of 4nbuB
- active site: G18 (= G18), N111 (= N112), S139 (= S140), Q149 (≠ G152), Y152 (= Y155), K156 (= K159)
- binding acetoacetyl-coenzyme a: D93 (≠ P94), K98 (≠ D99), S139 (= S140), N146 (≠ G147), V147 (≠ F150), Q149 (≠ G152), Y152 (= Y155), F184 (≠ L187), M189 (≠ I192), K200 (≠ E200)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), N17 (≠ S15), G18 (= G18), I19 (= I19), D38 (= D38), F39 (≠ L39), V59 (≠ C60), D60 (= D61), V61 (= V62), N87 (= N88), A88 (= A89), G89 (= G90), I90 (= I91), T137 (≠ M138), S139 (= S140), Y152 (= Y155), K156 (= K159), P182 (= P185), F184 (≠ L187), T185 (≠ I188), T187 (= T190), M189 (≠ I192)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
40% identity, 100% coverage: 1:248/249 of query aligns to 4:242/243 of 4i08A
- active site: G19 (= G18), N113 (= N112), S141 (= S140), Q151 (≠ G152), Y154 (= Y155), K158 (= K159)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R17), I20 (= I19), T40 (≠ L39), N62 (≠ D61), V63 (= V62), N89 (= N88), A90 (= A89), G140 (≠ S139), S141 (= S140), Y154 (= Y155), K158 (= K159), P184 (= P185), G185 (= G186), T189 (= T190)
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
39% identity, 100% coverage: 1:248/249 of query aligns to 1:243/244 of P0A2C9
- M125 (= M127) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (≠ V228) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (= S229) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
41% identity, 100% coverage: 1:248/249 of query aligns to 1:247/247 of 4jroC
- active site: G16 (= G18), S142 (= S140), Q152 (≠ G152), Y155 (= Y155), K159 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R17), G16 (= G18), I17 (= I19), N35 (vs. gap), Y36 (vs. gap), N37 (vs. gap), G38 (vs. gap), S39 (vs. gap), N63 (≠ H55), V64 (≠ L56), N90 (= N88), A91 (= A89), I93 (= I91), I113 (≠ V111), S142 (= S140), Y155 (= Y155), K159 (= K159), P185 (= P185), I188 (= I188), T190 (= T190)
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
39% identity, 99% coverage: 3:248/249 of query aligns to 2:242/243 of 7emgB
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
42% identity, 98% coverage: 1:245/249 of query aligns to 1:242/248 of Q9KJF1
- M1 (= M1) modified: Initiator methionine, Removed
- S15 (= S15) binding
- D36 (= D38) binding
- D62 (= D61) binding
- I63 (≠ V62) binding
- N89 (= N88) binding
- Y153 (= Y155) binding
- K157 (= K159) binding
7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
40% identity, 100% coverage: 2:249/249 of query aligns to 2:254/256 of 7do7A
- active site: G16 (= G18), S146 (= S140), Y159 (= Y155)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), R15 (= R17), G16 (= G18), I17 (= I19), S37 (≠ L39), D66 (= D61), A67 (≠ V62), N93 (= N88), A94 (= A89), G95 (= G90), I96 (= I91), V144 (≠ M138), S145 (= S139), S146 (= S140), Y159 (= Y155), K163 (= K159), P189 (= P185), G190 (= G186), I192 (= I188), T194 (= T190), I196 (= I192)
- binding beta-L-rhamnopyranose: F99 (≠ P94), S146 (= S140), S148 (= S142), Q156 (≠ G152), Y159 (= Y155), N197 (≠ T193), D235 (= D230), M236 (≠ L231), R238 (≠ T233)
7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
40% identity, 100% coverage: 2:249/249 of query aligns to 2:254/256 of 7b81A
- active site: G16 (= G18), S146 (= S140), Y159 (= Y155)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), S14 (≠ A14), R15 (= R17), I17 (= I19), D66 (= D61), A67 (≠ V62), N93 (= N88), A94 (= A89), G95 (= G90), I96 (= I91), T116 (≠ V111), V144 (≠ M138), S146 (= S140), Y159 (= Y155), K163 (= K159), P189 (= P185), G190 (= G186), I192 (= I188), T194 (= T190), I196 (= I192)
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
42% identity, 98% coverage: 3:245/249 of query aligns to 2:241/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), M16 (≠ I19), D35 (= D38), I36 (≠ L39), I62 (≠ V62), N88 (= N88), G90 (= G90), I138 (≠ M138), S140 (= S140), Y152 (= Y155), K156 (= K159), I185 (= I188)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
39% identity, 98% coverage: 3:246/249 of query aligns to 3:237/239 of 4nbtA
- active site: G16 (= G18), S132 (= S140), Y145 (= Y155), K149 (= K159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), K15 (≠ R17), G16 (= G18), L17 (≠ I19), D36 (= D38), L37 (= L39), L52 (≠ C60), N53 (≠ D61), V54 (= V62), N80 (= N88), A81 (= A89), G82 (= G90), I130 (≠ M138), S132 (= S140), Y145 (= Y155), K149 (= K159), P177 (= P185), G178 (= G186), I180 (= I188), T182 (= T190)
6wprA Crystal structure of a putative 3-oxoacyl-acp reductase (fabg) with NADP(h) from acinetobacter baumannii (see paper)
39% identity, 100% coverage: 1:248/249 of query aligns to 1:243/244 of 6wprA
- active site: G16 (= G18), S138 (= S140), Y151 (= Y155)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R17), T37 (≠ L39), L58 (≠ C60), D59 (= D61), V60 (= V62), N86 (= N88), A87 (= A89), G88 (= G90), I89 (= I91), I136 (≠ M138), Y151 (= Y155), K155 (= K159), P181 (= P185)
6t62A Crystal structure of acinetobacter baumannii fabg in complex with NADPH at 1.8 a resolution (see paper)
39% identity, 100% coverage: 1:248/249 of query aligns to 1:243/244 of 6t62A
- active site: G16 (= G18), S138 (= S140), Y151 (= Y155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R17), A36 (≠ D38), T37 (≠ L39), L58 (≠ C60), D59 (= D61), V60 (= V62), N86 (= N88), A87 (= A89), G88 (= G90), I89 (= I91), I136 (≠ M138), S137 (= S139), S138 (= S140), Y151 (= Y155), K155 (= K159), P181 (= P185), G182 (= G186), I184 (= I188), M188 (≠ I192)
3tzcA Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg)(y155f) from vibrio cholerae (see paper)
39% identity, 99% coverage: 1:246/249 of query aligns to 4:223/224 of 3tzcA
4cqmF Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD and NADP (see paper)
39% identity, 97% coverage: 5:246/249 of query aligns to 6:237/241 of 4cqmF
- active site: G17 (= G18), S139 (= S140), Q149 (≠ G152), Y152 (= Y155), K156 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G12), R16 (= R17), G17 (= G18), I18 (= I19), A37 (≠ D38), R38 (≠ L39), N39 (≠ R40), D60 (= D61), V61 (= V62), A87 (≠ N88), A88 (= A89), G89 (= G90), V137 (≠ M138), S139 (= S140), Y152 (= Y155), K156 (= K159), V185 (≠ I188), T187 (= T190), M189 (≠ I192)
Query Sequence
>H281DRAFT_06377 FitnessBrowser__Burk376:H281DRAFT_06377
MLLENKVVIVTGAASPRGIGKATAKALAAQGARVVILDLREEDAKAAAADLGAAHLGLAC
DVTNKAACVQAATATLERYGRIDGLVNNAGITQPVRTLDISAEGFDAIVDVNLRGTLYMS
QAVLPAMKEQKRGSIVCMSSVSAQRGGGIFGGPHYSAAKAGVLGLARAMAREFGPDSIRV
NSITPGLIQTDITGDKLTAEMRVDIIKGIPLGRLGDAADVANACLFLVSDLSTYLTGVTL
DVNGGMLIH
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory