SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS01150 FitnessBrowser__HerbieS:HSERO_RS01150 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
26% identity, 54% coverage: 2:236/433 of query aligns to 22:234/444 of Q8NLB7

query
sites
Q8NLB7

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D54 (≠ N30) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (= D36) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R88) mutation to A: Loss of transport activity.

Sites not aligning to the query:

309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

8et9A Cryo-em structure of the organic cation transporter 2 in complex with 1-methyl-4-phenylpyridinium (see paper)
27% identity, 44% coverage: 57:248/433 of query aligns to 143:311/517 of 8et9A

query
sites
8et9A

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binding 1-methyl-4-phenylpyridin-1-ium: W216 (≠ M141), Q240 (≠ L173), F243 (≠ A176)

Sites not aligning to the query:

binding 1-methyl-4-phenylpyridin-1-ium: 430

O15244 Solute carrier family 22 member 2; Organic cation transporter 2; hOCT2 from Homo sapiens (Human) (see 8 papers)
26% identity, 43% coverage: 61:248/433 of query aligns to 149:313/555 of O15244

query
sites
O15244

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M165 (≠ I80) to I: lower Vmax for MPP(+) transport; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177507
Y169 (≠ I84) mutation to F: No change in TEA uptake.
T201 (≠ E116) to M: in dbSNP:rs145450955
Y241 (≠ V172) mutation to F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-362 and F-377.
Y257 (≠ A189) mutation to F: No change in TEA uptake.
S270 (≠ G203) to A: decreased Ki value for TBA inhibition of MPP(+); no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs316019
Y279 (≠ H215) mutation to F: No change in TEA uptake.
Y280 (≠ H216) mutation to F: No change in TEA uptake.
P284 (≠ E218) mutation to A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
PESPR 284:288 (≠ EETPT 218:222) Proline-rich sequence
S286 (≠ T220) mutation to A: No change in TEA and metformin uptake. No change in tyrosine phosphorylation.
P287 (= P221) mutation to A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.

Sites not aligning to the query:

54 P → S: in dbSNP:rs8177504
73 Y→F: No change in TEA uptake.
92 Y→F: No change in TEA uptake.
128 Y→F: No change in TEA uptake.
362 Y→F: Decreased TEA uptake and YES1-mediated tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-241. Strong decrease in TEA uptake; when associated with F-377. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-377.
377 Y→F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-362.
400 R → C: lower Vmax and reduced Ki value for TBA inhibition of MPP(+); lower transport efficiency (Vmax/Km) and clearance of cyclo(his-pro); no change in transport efficiency (Vmax/Km) and clearance of salsolinol; dbSNP:rs8177516
432 K → Q: lower Km value for MPP(+) and reduced Ki value for TBA inhibition of MPP; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177517
458 Y→F: No change in TEA uptake.
544 Y→F: No change in TEA uptake.

8et8A Cryo-em structure of the organic cation transporter 1 in complex with verapamil (see paper)
29% identity, 39% coverage: 66:235/433 of query aligns to 152:299/532 of 8et8A

query
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8et8A

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binding (2S)-2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile: K213 (≠ G138), W216 (≠ M141), Q240 (= Q165)

Sites not aligning to the query:

binding (2S)-2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile: 31, 35, 353, 360, 378, 381, 385, 449, 469

8et7A Cryo-em structure of the organic cation transporter 1 in complex with diphenhydramine (see paper)
29% identity, 39% coverage: 66:235/433 of query aligns to 152:299/532 of 8et7A

query
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8et7A

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Sites not aligning to the query:

binding n-[2-(benzhydryloxy)ethyl]-n,n-dimethylamine: 35, 385, 445

8sc2A Human oct1 bound to diltiazem in inward-open conformation (see paper)
24% identity, 75% coverage: 57:381/433 of query aligns to 126:412/453 of 8sc2A

query
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8sc2A

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A

A

A

Y

A

A

L

L

A

P

S

H

W

W

G

R

W

W

-

L

R

Q

L

L

P

A

F

V

L

S

L

L

G

P

I

T

I

F

I

L

G

F

P

L

V

L

G

Y

Y

Y

Y

W

I

C

R

V

H

P

H

E

L

S

E

P

E

R

T

S

P

P

T

S

F

F

L

-

E

-

K

-

D

-

K

-

T

-

D

-

S

-

P

-

L

-

K

A

E

D

V

L

I

F

R

R

N

T

F

-

P

P

R

R

E

L

T

R

S

K

A

R

S

T

F

F

S

I

M

L

V

M

I

Y

L

L

W
|
W

T

F

V

T

C

D

T
x
S

Y

V

V

L

L

-

F

-

Y

-

M

-

P

-

T

-

Y

Y

S

Q

V

G

K

L

V

I

L

L

K

H

L

M

P

G

Q

A

T

T

T

S

G

G

-

N

-

L

F

Y

I

L

A

D

G

F

M

L

V

Y

G

S

G

A

L

L

C

V

I

E

M

I

V

P

L

G

A

A

P

F

I

I

V

A

G

L

R

I

L

T

A

I

D

D

R

R

I

V

G

G

R

R

-

I

-

Y

-

P

-

M

-

A

-

M

R

S

P

N

L

L

S

A

G

G

S

A

A

A

A

C

L

L

I

V

L

M

M

I

L

F

A

I

Y

S

P

P

M

D

F

L

A

H

Y

W

I

L

N

N

S

-

A

-

P

-

S

-

L

-

S

-

T

-

L

-

L

I

I

I

F

I

Q

M

L

C

V

V

F

G

G

R

T

M

L

G

I

I

A

T

A

I

Y

A

T
x
I

G

Q

P

M

I

I

L

C

A

L

A

V

F

N

S

A

E

E

L

L

F

Y

P

P

A

T

K

F

V

V

L

R

S

N

T

L

G

G

L

V

S

M

V

V

A

C

Y

S

N

S

L

L

A

C

binding Diltiazem: W199 (≠ M141), Q223 (≠ L173), F226 (≠ A176), W293 (= W255), S297 (≠ T259), I385 (≠ T354)

Sites not aligning to the query:

binding Diltiazem: 18

8ttgA Nora single mutant - e222q at ph 7.5
26% identity, 34% coverage: 54:200/433 of query aligns to 36:159/374 of 8ttgA

query
sites
8ttgA

T

T

G

G

N

S

D

D

L

L

T

G

S

-

L

-

L

L

A

L

V

V

A

A

T

A

F

F

G

A

V

L

G

S

-

Q

F

M

F

I

M

I

R

S

P

P

V

F

G

G

G
|
G

I
x
T

L

L

L

-

G

-

I

-

Y

-

A

A

D
|
D

R

K

V

L

G

G

R

K

K

K

A

L

A

I

L

I

S

C

V

I

T

G

I

L

L

I

L

L

M

F

A

S

V

V

G

S

T

E

T

F

M

M

I

F

G

A

L

V

A

G

P

H

T

N

Y

F

E

S

S

-

I

-

G

-

L

-

F

-

A

-

P

-

L

-

I

V

I

L

V

M

V

L

A

S

R

R

L

V

L

I

Q

G

G

G

F

M

S

S

A

A

G

G

G

M

E

V

M

M

G
x
P

S

G

A

V

T
|
T

A
x
G

F

L

L

I

T

A

E
x
D

Y

I

A

S

P

P

E

S

R

H

E

Q

R

K

A

A

Y

K

Y

N

S

F

S

G

W

Y

I

M

Q

S

A

A

S

I

I

I

G

N

V

S

A

G

V

F

L

I

L

L

G

G

A

P

A

G

V

I

G

G

T

G

F

F

V

-

T

-

A

-

S

-

L

-

D

-

A

-

L

M

A

A

S

E

W

V

G

S

W

H

R

R

L

M

P

P

F

F

binding : G59 (= G79), T60 (≠ I80), D63 (= D87), P110 (≠ G142), T113 (= T145), G114 (≠ A146), D118 (≠ E150)

Sites not aligning to the query:

binding : 183, 184, 315, 318, 319, 322

Q63089 Solute carrier family 22 member 1; Organic cation transporter 1; rOCT1 from Rattus norvegicus (Rat) (see 4 papers)
36% identity, 20% coverage: 66:151/433 of query aligns to 154:228/556 of Q63089

query
sites
Q63089

A

S

T
x
C

F

V

G

N

V

L

G

G

F

F

M

L

R

-

P

-

V

-

G

G

G

S

I

L

L

V

G

G

I

Y

Y

I

A

A

D

D

R

R

V

F

G

G

R

R

K

K

A

L

A
x
C

L

L

S

L

V

V

T

T

I

T

L

L

L

V

M

T

A

S

V

V

G

S

T

G

T

V

M

L

I

T

G

A

L

V

A

A

P

P

T

D

Y

Y

E

T

S

S

I

M

G

L

L

L

F

F

A

-

P

-

L

-

I

-

V

-

A

-

R

R

L

L

Q

Q

G

G

F
x
M

S
x
V

A
x
S

G
x
K

G
|
G

E
x
S

M
x
W

G
x
V

S
|
S

A
x
G

T
x
Y

A
x
T

F
x
L

L
x
I

T
|
T

E
|
E

Y
x
F

C155 (≠ T67) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-179; S-322; A-358; A-418; S-437; A-470 and A-474.
C179 (≠ A94) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; S-322; A-358; A-418; S-437; A-470 and A-474.
M212 (≠ F135) mutation to L: No change in TEA and MPP(+) uptake.
V213 (≠ S136) mutation to G: Decreased TEA uptake. No change in MPP(+) uptake.
S214 (≠ A137) mutation to G: Decreased TEA and MPP(+) uptake.
K215 (≠ G138) mutation to Q: Loss of TEA and MPP(+) uptake activity.; mutation to R: Loss of TEA and MPP(+) uptake activity.
G216 (= G139) mutation to A: Decreased TEA and MPP(+) uptake.
S217 (≠ E140) mutation to G: No change in TEA and MPP(+) uptake.
W218 (≠ M141) mutation to F: Decreased guanidine, histamine, serotonin and TEA uptake. No change in MPP(+) uptake. No change in TEA and MPP(+) affinity. Decreased TEA Vmax. No change in MPP(+) Vmax.; mutation to L: Decreased guanidine, histamine, serotonin, TEA and MPP(+) uptake. Decreased TEA affinity. No change in MPP(+) affinity. Decreased TEA and MPP(+) Vmax.; mutation to Y: Decreased guanidine, histamine, serotonin and TEA uptake. No change in MPP(+) uptake. Increased TEA and MPP(+) affinity. Decreased TEA and MPP(+) Vmax.
V219 (≠ G142) mutation to L: No change in TEA and MPP(+) uptake.
S220 (= S143) mutation to I: Decreased TEA and MPP(+) uptake.
G221 (≠ A144) mutation to A: Decreased TEA and MPP(+) uptake.
Y222 (≠ T145) mutation to F: No change in guanidine, histamine, serotonin, TEA and MPP(+) uptake. Increased TEA affinity. No change in MPP(+) affinity. Decreased TEA Vmax. No change in MPP(+) Vmax.; mutation to L: Decreased guanidine, serotonin, TEA and MPP(+) uptake. No change in histamine uptake. Increased TEA and MPP(+) affinity. Decreased TEA and MPP(+) Vmax.
T223 (≠ A146) mutation to I: Decreased TEA uptake. No change in MPP(+) uptake.
L224 (≠ F147) mutation to V: Decreased TEA and MPP(+) uptake.
I225 (≠ L148) mutation to G: No change in TEA and MPP(+) uptake.
T226 (= T149) mutation to A: Decreased TEA uptake. No change in MPP(+) uptake.
E227 (= E150) mutation to D: Loss of TEA and MPP(+) uptake activity.; mutation to Q: Loss of TEA and MPP(+) uptake activity.
F228 (≠ Y151) mutation to I: No change in TEA and MPP(+) uptake.

Sites not aligning to the query:

26 C→A: Choline affinity is increased fourfold by MMTS; when associated with A-155; A-179; S-322; A-358; A-418; S-437; A-470 and A-474.
229 V→A: Decreased TEA and MPP(+) uptake.; V→L: Loss of TEA and MPP(+) uptake activity.
286 S→A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-292; A-296; A-328 and A-550. No effect of PKA activation on ASP uptake. No effect of PKA activation on ASP uptake; when associated with A-292; A-296; A-328 and A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-292; A-296; A-328 and A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-292; A-296; A-328 and A-550. suppresses phosphorylation by PKC; when associated with A-292; A-296; A-328 and A-550.
292 S→A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-296; A-328 and A-550. No effect of PKA activation on ASP uptake. No effect of PKA activation on ASP uptake; when associated with A-286; A-296; A-328 and A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-296; A-328 and A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-296; A-328 and A-550. suppresses phosphorylation by PKC; when associated with A-286; A-296; A-328 and A-550.
296 T→A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-292; A-328; A-550. Significant increase of the ASP uptake by PKA activation. No effect of PKA activation on ASP uptake; when associated with A-286; A-292; A-328; A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-292; A-328; A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-292; A-328 and A-550. suppresses phosphorylation by PKC; when associated with A-286; A-292; A-328 and A-550.
322 C→S: Reduces the activation by MMTS. Abolishes the activation by MMTs; when associated with M-451. Choline affinity is increased fivefold by MMTS. Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; A-358; A-418; S-437; A-470 and A-474. Choline affinity is increased four- to fivefold; when associated with M-451.
328 S→A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-292; A-296 and A-550. No effect of PKA activation on ASP uptake. No effect of PKA activation on ASP uptake; when associated with A-286; A-292; A-296 and A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-292; A-296; A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-292; A-296 and A-550. suppresses phosphorylation by PKC; when associated with A-286; A-292; A-296 and A-550.
358 C→A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-418; S-437; A-470 and A-474.
418 C→A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; S-437; A-470 and A-474.
437 C→S: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; A-418; A-470 and A-474.
451 C→M: Reduces the activation by MMTS. Abolishes the activation by MMTs; when associated with S-322. Abolishes the effect of MMTs on choline-induced currents. Choline affinity is not influenced by MMTS. Choline affinity is increased four- to fivefold; when associated with S-322.
470 C→A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; A-418; A-437 and A-474.
474 C→A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; A-418; A-437 and A-470.
475 D→E: Decreased MPP(+) uptake, no change in MPP(+) affinity. Decreased NMN uptake, increased NMN affinity. Decreased choline uptake, increased choline affinity.; D→N: Decreased MPP(+) uptake.; D→R: Decreased MPP(+) uptake.
550 T→A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-292; A-296; A-328. Significant increase of the ASP uptake by PKA activation. No effect of PKA activation on ASP uptake; when associated with A-286; A-292; A-296 and A-328. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-292; A-296; A-328. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-292; A-296 and A-328. suppresses phosphorylation by PKC; when associated with A-286; A-292; A-296 and A-328.

8sc6A Human oct1 bound to thiamine in inward-open conformation (see paper)
24% identity, 75% coverage: 57:381/433 of query aligns to 126:405/447 of 8sc6A

query
sites
8sc6A

D

D

L

S

T

W

S

K

L

L

L

D

L

L

A

F

V

Q

A

S

T

C

F

L

G

N

V

A

G

G

F

F

F

L

M

F

R

G

P

S

V

L

G

G

G

-

I

-

L

-

L

V

G

G

I

Y

Y

F

A

A

D

D

R

R

V

F

G

G

R

R

K

K

A

L

A

C

L

L

S

L

V

G

T

T

I

V

L

L

L

V

M

N

A

A

V

V

G

S

T

G

T

V

M

L

I

M

G

A

L

F

A

S

P

P

T

N

Y

Y

E

M

S

S

I

M

G

L

L

L

F

F

A

-

P

-

L

-

I

-

V

-

A

-

R

R

L

L

Q

Q

G

G

F

L

S

V

A

S

G

K

G

G

E

N

M

W

G

M

S

A

A

G

T

Y

A

T

F

L

L

I

T

T

E

E

Y

F

A

V

P

G

E

S

R

G

E

S

R

R

A

-

Y

-

S

-

W

-

I

-

Q

-

A

R

S

T

I

V

G

A

V

I

A

M

V

Y

L
x
Q

L

M

G

A

A
x
F

A

T

V

V

G

G

T

L

F

V

V

A

T

L

A

T

S

G

L

L

D

-

A

A

A

Y

A

A

L

L

A

P

S

H

W

W

G

R

W

W

R

-

L

-

P

-

F

-

L

-

L

L

G

Q

I

L

I

A

I

V

G

-

P

-

V

-

G

-

Y

-

I

-

R

-

H

-

L

-

E

-

E

S

T

L

P

P

T

T

F

F

L

L

E

F

E

L

K

L

D

Y

K

Y

T

W

D

S

S

P

P

S

L

F

K

A

E

D

V

L

I

F

R

R

N

T

F

-

P

P

R

R

E

L

T

R

S

K

A

R

S

T

F

F

S

I

M

L

V

M

I

Y

L

L

W

W

T

F

V

T

C

D

T

S

Y

V

V

L

L

-

F

-

Y

-

M

-

P

-

T

-

Y
|
Y

S
x
Q

V

G

K

L

V

I

L

L

K

H

L

M

P

G

Q

A

T

T

T

S

G

G

-

N

-

L

F

Y

I

L

A

D

G

F

M

L

V

Y

G

S

G

A

L

L

C

V

I
x
E

M

I

V

P

L

G

A

A

P

F

I

I

V

A

G

L

R

I

L

T

A

I

D

D

R

R

I

V

G

G

R

R

-

I

-

Y

-

P

-

M

-

A

-

M

R

S

P

N

L

L

S

A

G

G

S

A

A

A

A

C

L

L

I

V

L

M

M

I

L

F

A

I

Y

S

P

P

M

D

F

L

A

H

Y

W

I

L

N

N

S

-

A

-

P

-

S

-

L

-

S

-

T

-

L

-

L

I

I

I

F

I

Q

M

L

C

V

V

F

G

G

R

T

M

L

G

I

I

A

T

A

I

Y

A

T
x
I

G
x
Q

P

M

I

I

L

C

A

L

A

V

F

N

S

A

E

E

L

L

F

Y

P

P

A

T

K

F

V

V

L

R

S

N

T

L

G

G

L

V

S

M

V

V

A

C

Y

S

N

S

L

L

A

C

binding 3-(4-amino-2-methyl-pyrimidin-5-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium: Q223 (≠ L173), F226 (≠ A176), Y293 (= Y269), Q294 (≠ S270), E318 (≠ I292), I378 (≠ T354), Q379 (≠ G355)

O08966 Solute carrier family 22 member 1; Organic cation transporter 1; mOCT1 from Mus musculus (Mouse) (see paper)
35% identity, 20% coverage: 66:151/433 of query aligns to 154:228/556 of O08966

query
sites
O08966

A

S

T

C

F

V

G

N

V

L

G

G

F

F

M

L

R

-

P

-

V

-

G

G

G

S

I

L

L

V

G

G

I

Y

Y

I

A

A

D

D

R

R

V

F

G

G

R

R

K

K

A

L

A

C

L

L

S

L

V

V

T

T

I

T

L

L

L

V

M

T

A

S

V

L

G

S

T

G

T

V

M

L

I

T

G

A

L

V

A

A

P

P

T

D

Y

Y

E

T

S

S

I

M

G

L

L

L

F

F

A

-

P

-

L

-

I

-

V

-

A

-

R

R

L

L

Q

Q

G

G

F

M

S

V

A

S

G

K

G

G

E

S

M

W

G

V

S

S

A

G

T

Y

A

T

F

L

L

I

T

T

E

E

Y

F

Sites not aligning to the query:

32 L→F: Increased trospium uptake. Increased trospium affinity. No change in fenoterol uptake.
36 Y→C: Decreased fenoterol uptake. Decreased fenoterol affinity. No change in trospium uptake. No change in terbutaline affinity.

8jtvA Hoct1 in complex with metformin in inward occluded conformation (see paper)
24% identity, 75% coverage: 57:381/433 of query aligns to 125:393/437 of 8jtvA

query
sites
8jtvA

D

D

L

S

T

W

S

K

L

L

L

D

L

L

A

F

V

Q

A

S

T

C

F

L

G

N

V

A

G

G

F

F

F

L

M

F

R

G

P

S

V

L

G

G

G

-

I

-

L

-

L

V

G

G

I

Y

Y

F

A

A

D

D

R

R

V

F

G

G

R

R

K

K

A

L

A

C

L

L

S

L

V

G

T

T

I

V

L

L

L

V

M

N

A

A

V

V

G

S

T

G

T

V

M

L

I

M

G

A

L

F

A

S

P

P

T

N

Y

Y

E

M

S

S

I

M

G

L

L

L

F

F

A

-

P

-

L

-

I

-

V

-

A

-

R

R

L

L

Q

Q

G

G

F

L

S

V

A

S

G

K

G

G

E

N

M
x
W

G

M

S

A

A

G

T

Y

A

T

F

L

L

I

T

T

E

E

Y

F

A

V

P

G

E

R

R

R

E

T

R

V

A

A

Y

I

Y

M

S

Y

S

Q

W

-

I

M

Q

A

A

F

S

T

I

V

G

G

V

L

A

V

V

A

L

L

L

T

G

G

A

L

A

A

V

Y

G

-

T

-

F

-

V

-

T

-

A

-

S

-

L

-

D

-

A

-

A

A

L

L

A

P

S

H

W

W

G

R

W

W

R

-

L

-

P

-

F

-

L

-

L

L

G

Q

I

L

I

A

I

V

G

-

P

-

V

-

G

-

Y

-

I

-

R

-

H

-

L

-

E

-

E

S

T

L

P

P

T

T

F

F

L

L

E

F

K

A

D

D

K

L

T

F

D

R

S

T

P

-

L

-

K

-

E

-

V

-

I

-

R

-

N

-

F

-

P

P

R

R

E

L

T

R

S

K

A

R

S

T

F

F

S

I

M

L

V

M

I

Y

L

L

W

W

T

F

V

T

C

D

T

S

Y

V

V

L

L

-

F

-

Y

-

M

-

P

-

T

-

Y
|
Y

S

Q

V

G

K

L

V

I

L

L

K

H

L

M

P

G

Q

A

T

T

T

S

G

G

-

N

-

L

F

Y

I

L

A

D

G

F

M

L

V

Y

G

S

G

A

L

L

C

V

I

E

M

I

V

P

L

G

A

A

P

F

I

I

V

A

G

L

R

I

L

T

A

I

D

D

R

R

I

V

G

G

R

R

-

I

-

Y

-

P

-

M

-

A

-

M

R

S

P

N

L

L

S

A

G

G

S

A

A

A

A

C

L

L

I

V

L

M

M

I

L

F

A

I

Y

S

P

P

M

D

F

L

A

H

Y

W

I

L

N

N

S

-

A

-

P

-

S

-

L

-

S

-

T

-

L

-

L

I

I

I

F

I

Q

M

L

C

V

V

F

G

G

R

T

M

L

G

I

I

A

T

A

I

Y

A

T

I

G

Q

P

M

I

I

L

C

A

L

A

V

F

N

S

A

E

E

L

L

F

Y

P

P

A

T

K

F

V

V

L

R

S

N

T

L

G

G

L

V

S

M

V

V

A

C

Y

S

N

S

L

L

A

C

binding Metformin: W198 (≠ M141), Y281 (= Y269)

8ju0A Hoct1 in complex with spironolactone in inward facing occluded conformation (see paper)
24% identity, 75% coverage: 57:381/433 of query aligns to 122:391/431 of 8ju0A

query
sites
8ju0A

D

D

L

S

T

W

S

K

L

L

L

D

L

L

A

F

V

Q

A

S

T

C

F

L

G

N

V

A

G

G

F

F

F

L

M

F

R

G

P

S

V

L

G

G

G

-

I

-

L

-

L

V

G

G

I

Y

Y

F

A

A

D

D

R

R

V

F

G

G

R

R

K

K

A

L

A

C

L

L

S

L

V

G

T

T

I

V

L

L

L

V

M

N

A

A

V

V

G

S

T

G

T

V

M

L

I

M

G

A

L

F

A

S

P

P

T

N

Y

Y

E

M

S

S

I

M

G

L

L

L

F

F

A

-

P

-

L

-

I

-

V

-

A

-

R

R

L

L

Q

Q

G

G

F

L

S

V

A

S

G
x
K

G

G

E

N

M
x
W

G

M

S

A

A

G

T

Y

A

T

F

L

L

I

T

T

E

E

Y

F

A

V

P

G

E

R

R

R

E

T

R

V

A

A

Y

I

Y

M

S

Y

S
x
Q

W

-

I

M

Q

A

A
x
F

S

T

I

V

G

G

V

L

A

V

V

A

L

L

L

T

G

G

A

L

A

A

V

Y

G

-

T

-

F

-

V

-

T

-

A

-

S

-

L

-

D

-

A

-

A

A

L

L

A

P

S

H

W

W

G

R

W

W

R

-

L

-

P

-

F

-

L

-

L

L

G

Q

I

L

I

A

I

V

G

-

P

-

V

-

G

-

Y

-

I

-

R

-

H

-

L

-

E

-

E

S

T

L

P

P

T

T

F

F

L

L

E

F

E

S

K

-

D

-

K

-

T

-

D

-

S

-

P

-

L

F

K

A

E

D

V

L

I

F

R

R

N

T

F

-

P

P

R

R

E

L

T

R

S

K

A

R

S

T

F

F

S

I

M

L

V

M

I

Y

L

L

W

W

T

F

V

T

C

D

T

S

Y

V

V

L

L

-

F

-

Y

-

M

-

P

-

T

-

Y
|
Y

S

Q

V

G

K

L

V

I

L

L

K

H

L

M

P

G

Q

A

T

T

T

S

G

G

-

N

-

L

F

Y

I

L

A

D

G

F

M

L

V

Y

G

S

G

A

L

L

C

V

I

E

M

I

V

P

L

G

A

A

P

F

I

I

V

A

G

L

R

I

L

T

A

I

D

D

R

R

I

V

G

G

R

R

-

I

-

Y

-

P

-

M

-

A

-

M

R

S

P

N

L

L

S

A

G

G

S

A

A

A

A

C

L

L

I

V

L

M

M

I

L

F

A

I

Y

S

P

P

M

D

F

L

A

H

Y

W

I

L

N

N

S

-

A

-

P

-

S

-

L

-

S

-

T

-

L

-

L

I

I

I

F

I

Q

M

L

C

V

V

F

G

G

R

T

M

L

G

I

I

A

T

A

I

Y

A

T

I

G
x
Q

P

M

I

I

L

C

A

L

A

V

F

N

S

A

E

E

L

L

F

Y

P

P

A

T

K

F

V

V

L

R

S

N

T

L

G

G

L

V

S

M

V

V

A

C

Y

S

N

S

L

L

A

C

binding spironolactone: K192 (≠ G138), W195 (≠ M141), Q216 (≠ S162), F219 (≠ A166), Y279 (= Y269), Q365 (≠ G355)

Sites not aligning to the query:

binding spironolactone: 10

8jttA Hoct1 in complex with metformin in outward occluded conformation (see paper)
24% identity, 75% coverage: 57:381/433 of query aligns to 66:395/454 of 8jttA

query
sites
8jttA

D

D

L

S

T

W

S

K

L

L

L

D

L

L

A

F

V

Q

A

S

T

C

F

L

G

N

V

A

G

G

F

F

F

L

M

F

R

G

P

S

V

L

G

G

G

-

I

-

L

-

L

V

G

G

I

Y

Y

F

A

A

D

D

R

R

V

F

G

G

R

R

K

K

A

L

A

C

L

L

S

L

V

G

T

T

I

V

L

L

L

V

M

N

A

A

V

V

G

S

T

G

T

V

M

L

I

M

G

A

L

F

A

S

P

P

T

N

Y

Y

E

M

S

S

I

M

G

L

L

L

F

F

A

-

P

-

L

-

I

-

V

-

A

-

R

R

L

L

Q

Q

G

G

F

L

S

V

A

S

G

K

G

G

E

N

M

W

G

M

S

A

A

G

T

Y

A

T

F

L

L

I

T

T

E

E

Y

F

A

V

P

G

E

S

R

G

E

S

R

R

A

R

Y

T

Y

V

S

A

S

I

W

M

I

Y

Q

Q

A

M

-

A

-

F

S

T

I

V

G

G

V

L

A

V

V

A

L

L

L

T

G

G

A

L

A

A

V

Y

G

A

-

L

-

P

-

H

-

W

-

R

-

W

T

L

F

Q

V

L

T

A

A

V

S

S

L

L

D

P

A

T

A

F

A

L

L

F

A

L

S

L

W

Y

G

Y

W

W

R

C

L

V

P

P

-

E

-

S

-

P

-

R

F

W

L

L

G

S

I

Q

I

K

I

R

G

N

P

T

V

E

G

A

Y

I

Y

K

I

I

R

M

H

D

H

H

L

I

E

A

E

Q

T

K

-

N

-

G

-

K

-

L

-

P

P

P

T

A

F

D

L

L

E

K

-

M

-

L

-

S

-

L

E

E

K

E

D

D

K

V

T

T

D

E

-

K

-

L

S

S

P

P

L

S

K

F

E

A

V

D

I

L

R

F

N

R

F

T

P

P

R

R

E

L

T

R

S

K

A

R

S

T

F

F

S

I

M

L

V

M

I

Y

L

L

W

W

T

F

V

T

C

D

T

S

Y

V

V

L

L

-

F

-

Y

-

M

-

P

-

T

-

Y
|
Y

S

Q

V

G

K

L

V

I

L

L

K

H

L

M

P

G

Q

A

T

T

T

S

G

G

-

N

-

L

F

Y

I

L

A

D

G

F

M

L

V

Y

G

S

G

A

L

L

C

V

I

E

M

I

V

P

L

G

A

A

P

F

I

I

V

A

G

L

R

I

L

T

A

I

D

D

R

R

I

V

G

G

R

R

-

I

-

Y

-

P

-

M

-

A

-

M

R

S

P

N

L

L

S

A

G

G

S

A

A

A

A

C

L

L

I

V

L

M

M

I

L

F

A

I

Y

S

P

P

M

D

F

L

A

H

Y

W

I

L

N

N

S

-

A

-

P

-

S

-

L

-

S

-

T

-

L

-

L

I

I

I

F

I

Q

M

L

C

V

V

F

G

G

R

T

M

L

G

I

I

A

T

A

I

Y

A

T

I

G

Q

P

M

I

I

L

C

A

L

A

V

F

N

S

A

E

E

L

L

F

Y

P

P

A

T

K

F

V

V

L

R

S

N

T

L

G

G

L

V

S

M

V

V

A

C

Y

S

N

S

L

L

A

C

binding Metformin: Y283 (= Y269)

Sites not aligning to the query:

binding Metformin: 34

5c65A Structure of the human glucose transporter glut3 / slc2a3
26% identity, 43% coverage: 53:240/433 of query aligns to 50:224/457 of 5c65A

query
sites
5c65A

P

P

T

S

G

E

N

V

D

L

L

L

T

T

S

S

L
|
L

L

W

-

S

L

L

A

S

V

V

A

A

T

I

F

F

G

S

V

V

G

G

F

G

F

M

M

-

R

-

P

-

V

I

G

G

G

S

I
x
F

L
x
S

L

V

G

G

I
x
L

Y

F

A

V

D

N

R

R

V

F

G

G

R

R

K
x
R

A

N

A

S

L

M

S

L

V

I

T

V

I

N

L
|
L

M

A

A

V

V

T

G

G

T

G

T

C

M

F

I

M

G

G

L

L

A

C

P

K

T

V

Y

A

E

K

S

S

I

V

G

E

L

M

F

-

A

-

P

-

L

-

I

-

I

L

V

I

V

L

A

G

R

R

L
|
L

L

V

Q

I

G

G

F

L

S

F

A

C

G

G

G

L

E

C

M

T

G

G

S

F

A

V

T

P

A

M

F

Y

L

I

T

G

E

E

Y

I

A

S

P

P

E

T

R

A

E

L

R

R

A

G

Y

A

Y

F

S

G

S

T

W

L

I

N

Q

Q

A

L

S

G

I

I

G

V

V

V

A

G

V

I

L

L

L

V

G

A

A

Q

A

I

V

F

G

G

-

L

T

E

F

F

V

I

T

L

A

G

S

S

L

E

D

E

A

L

A

-

L

-

A

-

S

-

W

W

G

-

W

-

R

-

L

-

P

P

F

L

L

L

G

G

I

F

I

T

I

I

G

L

P

P

-

A

-

I

V

L

G

Q

Y

S

Y

A

I

A

R

L

H
x
P

H

F

L

C

E

P

E

E

T

S

P

P

T

R

F

F

L

L

E

L

E

I

K

N

D

R

K

K

T

E

D

E

S

E

P

N

L

A

K

K

E

Q

V

I

I

L

R

Q

N

R

binding Octyl Glucose Neopentyl Glycol : L58 (= L61), F75 (≠ I80), S76 (≠ L81), L79 (≠ I84), R87 (≠ K92), L95 (= L100), L96 (= L101), L121 (= L131), P199 (≠ H215)

Sites not aligning to the query:

8sc3A Human oct1 bound to fenoterol in inward-open conformation (see paper)
24% identity, 75% coverage: 57:381/433 of query aligns to 126:403/445 of 8sc3A

query
sites
8sc3A

D

D

L

S

T

W

S

K

L

L

L

D

L

L

A

F

V

Q

A

S

T

C

F

L

G

N

V

A

G

G

F

F

F

L

M

F

R

G

P

S

V

L

G

G

G

-

I

-

L

-

L

V

G

G

I

Y

Y

F

A

A

D

D

R

R

V

F

G

G

R

R

K

K

A

L

A

C

L

L

S

L

V

G

T

T

I

V

L

L

L

V

M

N

A

A

V

V

G

S

T

G

T

V

M

L

I

M

G

A

L

F

A

S

P

P

T

N

Y

Y

E

M

S

S

I

M

G

L

L

L

F

F

A

-

P

-

L

-

I

-

V

-

A

-

R

R

L

L

Q

Q

G

G

F

L

S

V

A

S

G

K

G

G

E

N

M
x
W

G
x
M

S

A

A

G

T

Y

A

T

F

L

L

I

T

T

E

E

Y

F

A

V

P

G

E

S

R

G

E

S

R

R

A

-

Y

-

S

-

W

-

I

-

Q

-

A

R

S

T

I

V

G

A

V

I

A

M

V

Y

L
x
Q

L

M

G

A

A
x
F

A

T

V

V

G

G

T

L

F

V

V

A

T

L

A

T

S

G

L

L

D

-

A

A

A

Y

A

A

L

L

A

P

S

H

W

W

G

R

W

W

-

L

-

Q

-

L

-

A

-

V

R

S

L

L

P

P

F

T

L

F

L

L

G

F

I

L

I

-

G

-

P

-

V

-

G

-

Y

Y

I

-

R

-

H

-

L

-

E

-

T

-

P

P

T

S

F

F

L

-

E

-

K

-

D

-

K

-

T

-

D

-

S

-

P

-

L

-

K

A

E

D

V

L

I

F

R

R

N

T

F

-

P

P

R

R

E

L

T

R

S

K

A

R

S

T

F

F

S

I

M

L

V

M

I

Y

L

L

W
|
W

T

F

V

T

C

D

T

S

Y

V

V

L

L

-

F

-

Y

-

M

-

P

-

T

-

Y
|
Y

S

Q

V

G

K

L

V

I

L

L

K

H

L

M

P

G

Q

A

T

T

T

S

G

G

-

N

-

L

F

Y

I

L

A

D

G

F

M

L

V

Y

G

S

G

A

L

L

C

V

I

E

M

I

V

P

L

G

A

A

P

F

I

I

V

A

G

L

R

I

L

T

A

I

D

D

R

R

I

V

G

G

R

R

-

I

-

Y

-

P

-

M

-

A

-

M

R

S

P

N

L

L

S

A

G

G

S

A

A

A

A

C

L

L

I

V

L

M

M

I

L

F

A

I

Y

S

P

P

M

D

F

L

A

H

Y

W

I

L

N

N

S

-

A

-

P

-

S

-

L

-

S

-

T

-

L

-

L

I

I

I

F

I

Q

M

L

C

V

V

F

G

G

R

T

M

L

G

I

I

A

T

A

I

Y

A

T
x
I

G

Q

P

M

I

I

L
x
C

A

L

A

V

F

N

S

A

E

E

L

L

F

Y

P

P

A

T

K

F

V

V

L

R

S

N

T

L

G

G

L

V

S

M

V

V

A

C

Y

S

N

S

L

L

A

C

binding Fenoterol: W199 (≠ M141), M200 (≠ G142), Q223 (≠ L173), F226 (≠ A176), W284 (= W255), Y291 (= Y269), I376 (≠ T354), C380 (≠ L358)

8jtzA Hoct1 in complex with spironolactone in outward facing partially occluded conformation (see paper)
24% identity, 78% coverage: 57:393/433 of query aligns to 142:481/530 of 8jtzA

query
sites
8jtzA

D

D

L

S

T

W

S

K

L

L

L

D

L

L

A

F

V

Q

A

S

T

C

F

L

G

N

V

A

G

G

F

F

F

L

M

F

R

G

P

S

V

L

G

G

G

-

I

-

L

-

L

V

G

G

I

Y

Y

F

A

A

D

D

R

R

V

F

G

G

R

R

K

K

A

L

A

C

L

L

S

L

V

G

T

T

I

V

L

L

L

V

M

N

A

A

V

V

G

S

T

G

T

V

M

L

I

M

G

A

L

F

A

S

P

P

T

N

Y

Y

E

M

S

S

I

M

G

L

L

L

F

F

A

-

P

-

L

-

I

-

V

-

A

-

R

R

L

L

Q

Q

G

G

F

L

S

V

A

S

G
x
K

G

G

E

N

M
x
W

G

M

S

A

A

G

T

Y

A

T

F

L

L

I

T

T

E

E

Y

F

A

V

P

G

E

S

R

G

E

S

R

R

A

R

Y

T

Y

V

S

A

S

I

W

M

I

Y

Q
|
Q

A

M

-

A

-
x
F

S

T

I

V

G

G

V

L

A

V

V

A

L

L

L

T

G

G

A

L

A

A

V

Y

G

A

-

L

-

P

-

H

-

W

-

R

-

W

T

L

F

Q

V

L

T

A

A

V

S

S

L

L

D

P

A

T

A

F

A

L

L

F

A

L

S

L

W

Y

G

Y

W

W

R

C

L

V

P

P

-

E

-

S

-

P

-

R

F

W

L

L

G

S

I

Q

I

K

I

R

G

N

P

T

V

E

G

A

Y

I

Y

K

I

I

R

M

H

D

H

H

L

I

E

A

E

Q

T

K

-

N

-

G

-

K

-

L

-

P

P

P

T

A

F

D

L

L

E

K

-

M

-

L

-

S

-

L

E

E

K

E

D

D

K

V

T

T

D

E

-

K

-

L

S

S

P

P

L

S

K

F

E

A

V

D

I

L

R

F

N

R

F

T

P

P

R

R

E

L

T

R

S

K

A

R

S

T

F

F

S

I

M

L

V

M

I

Y

L

L

W

W

T

F

V

T

C

D

T

S

Y

V

V

L

L

-

F

-

Y

-

M

-

P

-

T

-

Y

Y

S

Q

V

G

K

L

V

I

L

L

K

H

L

M

P

G

Q

A

T

T

T

S

G

G

-

N

-

L

F

Y

I

L

A

D

G

F

M

L

V

Y

G

S

G

A

L

L

C

V

I

E

M

I

V

P

L

G

A

A

P

F

I

I

V

A

G

L

R

I

L

T

A

I

D

D

R

R

I

V

G

G

R

R

-

I

-

Y

-

P

-

M

-

A

-

M

R

S

P

N

L

L

S

A

G

G

S

A

A

A

A

C

L

L

I

V

L

M

M

I

L

F

A

I

Y

S

P

P

M

D

F

L

A

H

Y

W

I

L

N

N

S

-

A

-

P

-

S

-

L

-

S

-

T

-

L

-

L

I

I

I

F

I

Q

M

L

C

V

V

F

G

G

R

T

M

L

G

I

I

A
x
T

A

I

Y

A

T
x
I

G
x
Q

P

M

I

I

L

C

A

L

A

V

F

N

S

A

E

E

L

L

F

Y

P

P

A

T

K

F

V

V

L

R

S

N

T

L

G

G

L

V

S

M

V

V

A

C

Y

S

N

S

L

L

A
x
C

V

-

T

-

I

D

F

I

G

G

F

I

A

I

S

T

F

P

F

F

I

I

binding spironolactone: K212 (≠ G138), W215 (≠ M141), Q239 (= Q165), F242 (vs. gap), T441 (≠ A351), I444 (≠ T354), Q445 (≠ G355), C471 (≠ A381)

7sptA Crystal structure of exofacial state human glucose transporter glut3 (see paper)
26% identity, 43% coverage: 53:240/433 of query aligns to 54:228/470 of 7sptA

query
sites
7sptA

P

P

T

S

G

E

N

V

D

L

L

L

T

T

S

S

L

L

L

W

-

W

L

L

A

S

V

V

A

A

T

I

F

F

G

S

V

V

G

G

F

G

F

M

M

-

R

-

P

-

V

I

G

G

G

S

I

F

L

S

L

V

G

G

I

L

Y

F

A

V

D

N

R

R

V

F

G

G

R

R

K

R

A

N

A

S

L

M

S

L

V

I

T

V

I

N

L

L

M

A

A

V

V

T

G

G

T

G

T

C

M

F

I

M

G

G

L

L

A

C

P

K

T

V

Y

A

E

K

S

S

I

V

G

E

L

M

F

-

A

-

P

-

L

-

I

-

I

L

V

I

V

L

A

G

R

R

L

L

L

V

Q

I

G

G

F

L

S

F

A

C

G

G

G

L

E

C

M

T

G

G

S

F

A

V

T

P

A

M

F

Y

L

I

T

G

E

E

Y

I

A

S

P

P

E

T

R

A

E

L

R

R

A

G

Y

A

Y

F

S

G

S

T

W

L

I

N

Q
|
Q

A

L

S

G

I

I

G

V

V

V

A

G

V
x
I

L

L

L

V

G

A

A

Q

A

I

V

F

G

G

-

L

T

E

F

F

V

I

T

L

A

G

S

S

L

E

D

E

A

L

A

-

L

-

A

-

S

-

W

W

G

-

W

-

R

-

L

-

P

P

F

L

L

L

G

G

I

F

I

T

I

I

G

L

P

P

-

A

-

I

V

L

G

Q

Y

S

Y

A

I

A

R

L

H

P

H

F

L

C

E

P

E

E

T

S

P

P

T

R

F

F

L

L

E

L

E

I

K

N

D

R

K

K

T

E

D

E

S

E

P

N

L

A

K

K

E

Q

V

I

I

L

R

Q

N

R

binding alpha-D-glucopyranose: Q159 (= Q165), I166 (≠ V172)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 24, 280, 281, 286, 386

7crzA Crystal structure of human glucose transporter glut3 bound with c3361 (see paper)
26% identity, 43% coverage: 53:240/433 of query aligns to 52:226/469 of 7crzA

query
sites
7crzA

P

P

T

S

G

E

N

V

D

L

L

L

T

T

S

S

L

L

L

W

-

S

L

L

A

S

V

V

A
|
A

T

I

F

F

G
x
S

V

V

G

G

F

G

F

M

M

-

R

-

P

-

V

I

G

G

G

S

I

F

L

S

L

V

G

G

I

L

Y

F

A

V

D

N

R

R

V

F

G

G

R

R

K

R

A

N

A

S

L

M

S

L

V

I

T

V

I

N

L

L

M

A

A

V

V

T

G

G

T

G

T

C

M

F

I

M

G

G

L

L

A

C

P

K

T

V

Y

A

E

K

S

S

I

V

G

E

L

M

F

-

A

-

P

-

L

-

I

-

I

L

V

I

V

L

A

G

R

R

L

L

L

V

Q

I

G

G

F

L

S

F

A

C

G

G

G

L

E

C

M

T

G

G

S

F

A

V

T

P

A

M

F

Y

L

I

T

G

E

E

Y

I

A

S

P

P

E

T

R

A

E

L

R

R

A

G

Y

A

Y

F

S

G

S

T

W

L

I

N

Q
|
Q

A

L

S

G

I

I

G

V

V

V

A

G

V
x
I

L

L

L

V

G

A

A

Q

A

I

V

F

G

G

-

L

T

E

F

F

V

I

T

L

A

G

S

S

L

E

D

E

A

L

A

-

L

-

A

-

S

-

W

W

G

-

W

-

R

-

L

-

P

P

F

L

L

L

G

G

I

F

I

T

I

I

G

L

P

P

-

A

-

I

V

L

G

Q

Y

S

Y

A

I

A

R

L

H

P

H

F

L

C

E

P

E

E

T

S

P

P

T

R

F

F

L

L

E

L

E

I

K

N

D

R

K

K

T

E

D

E

S

E

P

N

L

A

K

K

E

Q

V

I

I

L

R

Q

N

R

binding (2S,3R,4S,5R,6R)-6-(hydroxymethyl)-4-undec-10-enoxy-oxane-2,3,5-triol: A66 (= A66), S69 (≠ G69), Q157 (= Q165), I164 (≠ V172)

Sites not aligning to the query:

binding (2S,3R,4S,5R,6R)-6-(hydroxymethyl)-4-undec-10-enoxy-oxane-2,3,5-triol: 26, 278, 279, 284, 313, 375, 384, 411, 412, 415

7spsA Crystal structure of human glucose transporter glut3 bound with exofacial inhibitor sa47 (see paper)
26% identity, 43% coverage: 53:240/433 of query aligns to 51:225/468 of 7spsA

query
sites
7spsA

P

P

T

S

G

E

N

V

D

L

L

L

T

T

S

S

L

L

L

W

-

S

L

L

A

S

V

V

A

A

T

I

F

F

G

S

V

V

G

G

F

G

F

M

M

-

R

-

P

-

V

I

G

G

G

S

I

F

L

S

L

V

G

G

I

L

Y

F

A

V

D

N

R

R

V

F

G

G

R

R

K

R

A

N

A

S

L

M

S

L

V

I

T

V

I

N

L

L

M

A

A

V

V

T

G

G

T

G

T

C

M

F

I

M

G

G

L

L

A

C

P

K

T

V

Y

A

E

K

S

S

I

V

G

E

L

M

F

-

A

-

P

-

L

-

I

-

I

L

V

I

V

L

A

G

R

R

L

L

L

V

Q

I

G

G

F

L

S

F

A

C

G

G

G

L

E

C

M

T

G

G

S

F

A

V

T

P

A

M

F

Y

L

I

T

G

E

E

Y

I

A

S

P

P

E

T

R

A

E

L

R

R

A

G

Y

A

Y

F

S

G

S

T

W

L

I

N

Q
|
Q

A

L

S

G

I

I

G

V

V

V

A

G

V
x
I

L

L

L

V

G

A

A

Q

A

I

V

F

G

G

-

L

T

E

F

F

V

I

T

L

A

G

S

S

L

E

D

E

A

L

A

-

L

-

A

-

S

-

W

W

G

-

W

-

R

-

L

-

P

P

F

L

L

L

G

G

I

F

I

T

I

I

G

L

P

P

-

A

-

I

V

L

G

Q

Y

S

Y

A

I

A

R

L

H

P

H

F

L

C

E

P

E

E

T

S

P

P

T

R

F

F

L

L

E

L

E

I

K

N

D

R

K

K

T

E

D

E

S

E

P

N

L

A

K

K

E

Q

V

I

I

L

R

Q

N

R

binding methyl N-[(2-{4-[4-(5-fluoro-2-methoxyphenyl)piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidin-1-yl}phenyl)methyl]-beta-alaninate: Q156 (= Q165), I163 (≠ V172)

Sites not aligning to the query:

binding methyl N-[(2-{4-[4-(5-fluoro-2-methoxyphenyl)piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidin-1-yl}phenyl)methyl]-beta-alaninate: 21, 25, 29, 278, 286, 308, 312, 374, 375, 406, 407, 410

4zw9A Crystal structure of human glut3 bound to d-glucose in the outward- occluded conformation at 1.5 angstrom (see paper)
26% identity, 43% coverage: 53:240/433 of query aligns to 54:228/470 of 4zw9A

query
sites
4zw9A

P

P

T

S

G

E

N

V

D

L

L

L

T

T

S

S

L

L

L

W

-

S

L

L

A

S

V

V

A

A

T

I

F

F

G

S

V

V

G

G

F

G

F

M

M

-

R

-

P

-

V

I

G

G

G

S

I

F

L

S

L

V

G

G

I

L

Y

F

A

V

D

N

R

R

V

F

G

G

R

R

K

R

A

N

A

S

L

M

S

L

V

I

T

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