SitesBLAST
Comparing HSERO_RS01305 FitnessBrowser__HerbieS:HSERO_RS01305 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
38% identity, 97% coverage: 5:390/397 of query aligns to 3:398/408 of P38502
- N7 (= N9) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
- S10 (= S12) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
- S12 (≠ T14) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
- K14 (= K16) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
- R91 (= R91) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
- V93 (= V93) mutation to A: Decreases affinity for acetate.
- L122 (= L122) mutation to A: Decreases affinity for acetate.
- D148 (= D148) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
- F179 (= F178) mutation to A: Decreases affinity for acetate.
- N211 (= N209) mutation to A: Slightly reduced enzyme activity.
- P232 (≠ T230) mutation to A: Decreases affinity for acetate.
- R241 (= R239) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
- E384 (= E376) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.
1tuuB Acetate kinase crystallized with atpgs (see paper)
38% identity, 97% coverage: 5:390/397 of query aligns to 3:398/398 of 1tuuB
- active site: N7 (= N9), R91 (= R91), H180 (= H179), R241 (= R239), E384 (= E376)
- binding adenosine monophosphate: D283 (= D280), R285 (= R282), G331 (= G325), I332 (= I326), N335 (≠ H329), S336 (≠ D330)
- binding trihydrogen thiodiphosphate: H180 (= H179), G212 (= G210), R241 (= R239)
1tuuA Acetate kinase crystallized with atpgs (see paper)
38% identity, 97% coverage: 5:390/397 of query aligns to 3:398/399 of 1tuuA
- active site: N7 (= N9), R91 (= R91), H180 (= H179), R241 (= R239), E384 (= E376)
- binding adenosine-5'-diphosphate: K14 (= K16), G210 (= G208), D283 (= D280), F284 (≠ V281), R285 (= R282), G331 (= G325), I332 (= I326), N335 (≠ H329)
- binding sulfate ion: R91 (= R91), H180 (= H179), G212 (= G210)
7fj9A Kpacka (pduw) with amppnp complex structure
40% identity, 97% coverage: 5:389/397 of query aligns to 4:393/395 of 7fj9A
7fj8A Kpacka (pduw) with amp complex structure
40% identity, 97% coverage: 5:389/397 of query aligns to 4:393/395 of 7fj8A
4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
38% identity, 97% coverage: 4:387/397 of query aligns to 3:389/394 of 4fwsA
- active site: N8 (= N9), R83 (= R91), H172 (= H179), R233 (= R239), E378 (= E376)
- binding cytidine-5'-triphosphate: G202 (= G208), N203 (= N209), G204 (= G210), D275 (= D280), L276 (≠ V281), R277 (= R282), G323 (= G325), I324 (= I326), N327 (≠ H329)
- binding 1,2-ethanediol: V21 (≠ G24), C24 (≠ A27), H115 (= H123), N203 (= N209), T232 (= T238), R233 (= R239), K262 (≠ H267)
4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
38% identity, 97% coverage: 4:387/397 of query aligns to 3:389/394 of 4fwrA
- active site: N8 (= N9), R83 (= R91), H172 (= H179), R233 (= R239), E378 (= E376)
- binding cytidine-5'-monophosphate: G202 (= G208), N203 (= N209), D275 (= D280), L276 (≠ V281), R277 (= R282), G323 (= G325), I324 (= I326), N327 (≠ H329)
4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
38% identity, 97% coverage: 4:387/397 of query aligns to 3:389/394 of 4fwqA
- active site: N8 (= N9), R83 (= R91), H172 (= H179), R233 (= R239), E378 (= E376)
- binding guanosine-5'-triphosphate: H172 (= H179), N203 (= N209), G204 (= G210), D275 (= D280), L276 (≠ V281), R277 (= R282), E280 (≠ Q285), G323 (= G325), I324 (= I326), N327 (≠ H329)
4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
38% identity, 97% coverage: 4:387/397 of query aligns to 3:389/394 of 4fwpA
- active site: N8 (= N9), R83 (= R91), H172 (= H179), R233 (= R239), E378 (= E376)
- binding 1,2-ethanediol: S11 (= S12), H115 (= H123), K262 (≠ H267)
- binding guanosine-5'-diphosphate: N203 (= N209), D275 (= D280), L276 (≠ V281), R277 (= R282), E280 (≠ Q285), G323 (= G325), I324 (= I326), N327 (≠ H329), S328 (≠ D330)
4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
38% identity, 97% coverage: 4:387/397 of query aligns to 3:389/394 of 4fwoA
- active site: N8 (= N9), R83 (= R91), H172 (= H179), R233 (= R239), E378 (= E376)
- binding guanosine-5'-monophosphate: G202 (= G208), N203 (= N209), D275 (= D280), L276 (≠ V281), R277 (= R282), E280 (≠ Q285), G323 (= G325), I324 (= I326), N327 (≠ H329)
- binding 1,2-ethanediol: E100 (≠ A108), N104 (≠ E112)
4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
38% identity, 97% coverage: 4:387/397 of query aligns to 3:389/394 of 4fwnA
- active site: N8 (= N9), R83 (= R91), H172 (= H179), R233 (= R239), E378 (= E376)
- binding adenosine-5'-tetraphosphate: H172 (= H179), H200 (= H206), N203 (= N209), G204 (= G210), D275 (= D280), L276 (≠ V281), R277 (= R282), G323 (= G325), I324 (= I326), N327 (≠ H329)
4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
38% identity, 97% coverage: 4:387/397 of query aligns to 3:389/394 of 4fwmA