SitesBLAST
Comparing HSERO_RS01420 FitnessBrowser__HerbieS:HSERO_RS01420 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
67% identity, 97% coverage: 10:325/326 of query aligns to 7:322/323 of O59791
- S82 (= S85) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
67% identity, 97% coverage: 10:325/326 of query aligns to 2:317/318 of 1wtcA
- active site: K52 (= K60), S77 (= S85), E203 (= E211), G207 (= G215), D209 (= D217), G231 (= G239), I302 (= I310), S303 (= S311)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ H28), K47 (≠ R55), M48 (= M56), A109 (= A117), A110 (= A118), Y114 (= Y122)
- binding magnesium ion: E203 (= E211), G207 (= G215), D209 (= D217)
- binding pyridoxal-5'-phosphate: F51 (= F59), K52 (= K60), N79 (= N87), G178 (= G186), G179 (= G187), G180 (= G188), G181 (= G189), G231 (= G239), E276 (= E284), T278 (= T286), S303 (= S311)
1v71A Crystal structure of s.Pombe serine racemase
67% identity, 97% coverage: 10:325/326 of query aligns to 2:317/318 of 1v71A
- active site: K52 (= K60), S77 (= S85), E203 (= E211), G207 (= G215), D209 (= D217), G231 (= G239), I302 (= I310), S303 (= S311)
- binding magnesium ion: E203 (= E211), G207 (= G215), D209 (= D217)
- binding pyridoxal-5'-phosphate: F51 (= F59), K52 (= K60), N79 (= N87), G178 (= G186), G179 (= G187), G180 (= G188), G181 (= G189), G231 (= G239), E276 (= E284), T278 (= T286), S303 (= S311), G304 (= G312)
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
67% identity, 97% coverage: 10:325/326 of query aligns to 3:318/319 of 2zr8A
- active site: K53 (= K60), S78 (= S85), E204 (= E211), G208 (= G215), D210 (= D217), G232 (= G239), I303 (= I310), S304 (= S311)
- binding magnesium ion: E204 (= E211), G208 (= G215), D210 (= D217)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F59), K53 (= K60), S77 (= S84), S78 (= S85), N80 (= N87), H81 (= H88), P147 (= P154), G179 (= G186), G180 (= G187), G181 (= G188), G182 (= G189), G232 (= G239), E277 (= E284), T279 (= T286), S304 (= S311)
- binding serine: S78 (= S85), R129 (= R136), D231 (= D238), G232 (= G239), A233 (= A240), Q234 (= Q241), T235 (= T242)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
67% identity, 97% coverage: 10:325/326 of query aligns to 3:318/319 of 2zpuA
- active site: K53 (= K60), S78 (= S85), E204 (= E211), G208 (= G215), D210 (= D217), G232 (= G239), I303 (= I310), S304 (= S311)
- binding magnesium ion: E204 (= E211), G208 (= G215), D210 (= D217)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F59), K53 (= K60), S77 (= S84), S78 (= S85), N80 (= N87), H81 (= H88), P147 (= P154), G179 (= G186), G180 (= G187), G181 (= G188), G182 (= G189), G232 (= G239), E277 (= E284), T279 (= T286), S304 (= S311)
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
70% identity, 97% coverage: 9:324/326 of query aligns to 2:317/319 of A4F2N8
- K53 (= K60) mutation to A: Loss of enzymatic activity.
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
45% identity, 94% coverage: 19:324/326 of query aligns to 27:336/339 of Q7XSN8
- E219 (= E211) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (= D217) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
43% identity, 96% coverage: 12:324/326 of query aligns to 5:323/323 of 7nbfAAA
- active site: K53 (= K60), S81 (= S85), E207 (= E211), A211 (≠ G215), D213 (= D217), G236 (= G239), L309 (≠ I310), S310 (= S311)
- binding calcium ion: E207 (= E211), A211 (≠ G215), D213 (= D217)
- binding magnesium ion: N244 (≠ Y248)
- binding pyridoxal-5'-phosphate: F52 (= F59), K53 (= K60), N83 (= N87), G182 (= G186), G183 (= G187), G184 (= G188), G185 (= G189), M186 (≠ L190), G236 (= G239), V237 (≠ A240), T282 (= T286), S310 (= S311), G311 (= G312)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (= H28), L22 (≠ R29), T23 (= T30), P24 (= P31), L26 (= L33), T27 (≠ R34), F46 (= F53)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
43% identity, 96% coverage: 12:324/326 of query aligns to 5:323/323 of 7nbdAAA
- active site: K53 (= K60), S81 (= S85), E207 (= E211), A211 (≠ G215), D213 (= D217), G236 (= G239), L309 (≠ I310), S310 (= S311)
- binding calcium ion: E207 (= E211), A211 (≠ G215), D213 (= D217)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ A276), L278 (≠ V282), V314 (= V315), L316 (= L317)
- binding magnesium ion: N244 (≠ Y248)
- binding pyridoxal-5'-phosphate: F52 (= F59), K53 (= K60), N83 (= N87), G182 (= G186), G183 (= G187), G184 (= G188), G185 (= G189), M186 (≠ L190), G236 (= G239), V237 (≠ A240), E280 (= E284), T282 (= T286), S310 (= S311), G311 (= G312)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
43% identity, 96% coverage: 12:324/326 of query aligns to 5:323/323 of 7nbcCCC
- active site: K53 (= K60), S81 (= S85), E207 (= E211), A211 (≠ G215), D213 (= D217), G236 (= G239), L309 (≠ I310), S310 (= S311)
- binding biphenyl-4-ylacetic acid: T78 (= T82), H79 (≠ F83), H84 (= H88), V148 (≠ I152), H149 (≠ P153), P150 (= P154)
- binding calcium ion: E207 (= E211), A211 (≠ G215), D213 (= D217)
- binding pyridoxal-5'-phosphate: F52 (= F59), K53 (= K60), N83 (= N87), G182 (= G186), G183 (= G187), G184 (= G188), G185 (= G189), M186 (≠ L190), G236 (= G239), V237 (≠ A240), T282 (= T286), S310 (= S311), G311 (= G312)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
43% identity, 96% coverage: 12:324/326 of query aligns to 5:323/323 of 7nbcAAA
- active site: K53 (= K60), S81 (= S85), E207 (= E211), A211 (≠ G215), D213 (= D217), G236 (= G239), L309 (≠ I310), S310 (= S311)
- binding calcium ion: E207 (= E211), A211 (≠ G215), D213 (= D217)
- binding magnesium ion: N244 (≠ Y248)
- binding pyridoxal-5'-phosphate: F52 (= F59), K53 (= K60), N83 (= N87), G182 (= G186), G183 (= G187), G184 (= G188), G185 (= G189), M186 (≠ L190), G236 (= G239), V237 (≠ A240), T282 (= T286), S310 (= S311), G311 (= G312)
Sites not aligning to the query:
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
43% identity, 96% coverage: 12:323/326 of query aligns to 5:322/322 of 7nbgAAA
- active site: K53 (= K60), S81 (= S85), E207 (= E211), A211 (≠ G215), D213 (= D217), G236 (= G239), L309 (≠ I310), S310 (= S311)
- binding calcium ion: E207 (= E211), A211 (≠ G215), D213 (= D217)
- binding pyridoxal-5'-phosphate: F52 (= F59), K53 (= K60), N83 (= N87), G182 (= G186), G183 (= G187), G184 (= G188), G185 (= G189), M186 (≠ L190), G236 (= G239), V237 (≠ A240), T282 (= T286), S310 (= S311), G311 (= G312)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (= S85), G85 (≠ A89), Q86 (= Q90), I101 (= I105), K111 (= K115), I115 (≠ T119), Y118 (= Y122)
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
43% identity, 94% coverage: 12:317/326 of query aligns to 5:316/320 of 7nbhAAA
- active site: K53 (= K60), S81 (= S85), E207 (= E211), A211 (≠ G215), D213 (= D217), G236 (= G239), L309 (≠ I310), S310 (= S311)
- binding calcium ion: E207 (= E211), A211 (≠ G215), D213 (= D217)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (= S85), G85 (≠ A89), Q86 (= Q90), K111 (= K115), I115 (≠ T119), Y118 (= Y122), D235 (= D238), P281 (= P285), N313 (= N314), V314 (= V315), D315 (= D316)
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
42% identity, 96% coverage: 12:324/326 of query aligns to 6:319/322 of 3l6bA
- active site: K54 (= K60), S77 (= S85), E203 (= E211), A207 (≠ G215), D209 (= D217), G232 (= G239), T278 (= T286), L305 (≠ I310), S306 (= S311)
- binding malonate ion: K54 (= K60), S76 (= S84), S77 (= S85), N79 (= N87), H80 (= H88), R128 (= R136), G232 (= G239)
- binding manganese (ii) ion: E203 (= E211), A207 (≠ G215), D209 (= D217)
- binding pyridoxal-5'-phosphate: F53 (= F59), K54 (= K60), N79 (= N87), G178 (= G186), G179 (= G187), G180 (= G188), G181 (= G189), M182 (≠ L190), V233 (≠ A240), E276 (= E284), T278 (= T286), S306 (= S311), G307 (= G312)
6zspAAA serine racemase bound to atp and malonate. (see paper)
42% identity, 96% coverage: 12:324/326 of query aligns to 5:316/320 of 6zspAAA
- active site: K53 (= K60), S74 (= S85), E200 (= E211), A204 (≠ G215), D206 (= D217), G229 (= G239), L302 (≠ I310), S303 (= S311)
- binding adenosine-5'-triphosphate: S28 (= S35), S29 (≠ R36), I30 (≠ T37), K48 (≠ R55), T49 (≠ M56), Q79 (= Q90), Y111 (= Y122), E266 (= E277), R267 (= R278), K269 (= K280), N306 (= N314)
- binding magnesium ion: E200 (= E211), A204 (≠ G215), D206 (= D217)
- binding malonate ion: K53 (= K60), S73 (= S84), S74 (= S85), N76 (= N87), H77 (= H88), R125 (= R136), G229 (= G239), S232 (≠ T242)
7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
42% identity, 94% coverage: 12:316/326 of query aligns to 5:310/310 of 7nbgDDD
- active site: K53 (= K60), S76 (= S85), E202 (= E211), A206 (≠ G215), D208 (= D217), G231 (= G239), L304 (≠ I310), S305 (= S311)
- binding calcium ion: E202 (= E211), A206 (≠ G215), D208 (= D217)
- binding magnesium ion: N239 (≠ Y248)
- binding ortho-xylene: S76 (= S85), Q81 (= Q90), I96 (= I105), Y113 (= Y122)
- binding pyridoxal-5'-phosphate: F52 (= F59), K53 (= K60), N78 (= N87), G177 (= G186), G178 (= G187), G179 (= G188), G180 (= G189), M181 (≠ L190), G231 (= G239), V232 (≠ A240), E275 (= E284), T277 (= T286), S305 (= S311), G306 (= G312)
Sites not aligning to the query:
5cvcA Structure of maize serine racemase (see paper)
45% identity, 93% coverage: 19:320/326 of query aligns to 11:316/329 of 5cvcA
- active site: K52 (= K60), S77 (= S85), E203 (= E211), A207 (≠ G215), D209 (= D217), G231 (= G239), V306 (≠ I310), S307 (= S311)
- binding magnesium ion: E203 (= E211), A207 (≠ G215), D209 (= D217)
- binding pyridoxal-5'-phosphate: F51 (= F59), K52 (= K60), N79 (= N87), S178 (≠ G186), G179 (= G187), G180 (= G188), G181 (= G189), L232 (≠ A240), E275 (= E284), S307 (= S311), G308 (= G312)
3hmkA Crystal structure of serine racemase (see paper)
42% identity, 95% coverage: 12:321/326 of query aligns to 6:321/321 of 3hmkA
- active site: K54 (= K60), S82 (= S85), E208 (= E211), A212 (≠ G215), D214 (= D217), G237 (= G239), T283 (= T286), L310 (≠ I310), S311 (= S311)
- binding manganese (ii) ion: E208 (= E211), A212 (≠ G215), D214 (= D217)
- binding pyridoxal-5'-phosphate: F53 (= F59), K54 (= K60), N84 (= N87), G183 (= G186), G184 (= G187), G185 (= G188), G186 (= G189), M187 (≠ L190), G237 (= G239), V238 (≠ A240), T283 (= T286), S311 (= S311), G312 (= G312)
Q9QZX7 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Mus musculus (Mouse) (see paper)
43% identity, 95% coverage: 12:320/326 of query aligns to 8:322/339 of Q9QZX7
- C113 (≠ A114) modified: S-nitrosocysteine; mutation to S: Abolishes S-nitrosylation.
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
42% identity, 94% coverage: 11:317/326 of query aligns to 1:306/308 of 1ve5A
- active site: K50 (= K60), S56 (≠ T69), S72 (= S85), E200 (= E211), A204 (≠ G215), D206 (= D217), G229 (= G239), L299 (≠ I310), S300 (= S311)
- binding calcium ion: E200 (= E211), A204 (≠ G215), D206 (= D217)
- binding pyridoxal-5'-phosphate: F49 (= F59), K50 (= K60), N74 (= N87), G175 (vs. gap), G176 (vs. gap), G177 (vs. gap), G178 (vs. gap), E274 (= E284), T276 (= T286), S300 (= S311), G301 (= G312)
Query Sequence
>HSERO_RS01420 FitnessBrowser__HerbieS:HSERO_RS01420
MIPVSSATELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFK
FRGAMNALTQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATR
GYGAEVVTYDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDA
LFVCLGGGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGA
QTQHLGKYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQ
LQGKRVGVIISGGNVDLGKLATFLAD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory