SitesBLAST
Comparing HSERO_RS01610 FitnessBrowser__HerbieS:HSERO_RS01610 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
35% identity, 93% coverage: 11:370/386 of query aligns to 5:345/362 of 3bptA
- active site: G67 (= G73), P84 (≠ A101), R88 (≠ E105), G115 (= G132), G118 (= G135), E138 (= E155), D146 (= D163)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G72), G67 (= G73), I69 (≠ L75), E90 (= E107), G114 (= G131), G115 (= G132), E138 (= E155), D146 (= D163), V147 (= V164)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ T31), L26 (= L32), A28 (= A34), G66 (= G72), G67 (= G73), I69 (≠ L75), P137 (= P154), I141 (= I158), L319 (≠ I344)
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
30% identity, 91% coverage: 21:370/386 of query aligns to 19:353/378 of Q9LKJ1
- G70 (= G73) mutation to S: Loss of activity.
- E142 (= E155) mutation to A: Loss of activity.
- D150 (= D163) mutation to G: Reduced activity.
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
34% identity, 91% coverage: 21:373/386 of query aligns to 12:333/340 of 4hdtA
- active site: G64 (= G73), I69 (≠ L78), W84 (≠ F104), Y88 (= Y108), G112 (= G132), G115 (= G135), E135 (= E155), P142 (= P162), D143 (= D163), R283 (= R321)
- binding zinc ion: H28 (≠ L37), E42 (≠ Q51), E57 (= E66), E79 (≠ S88), H93 (≠ L113), H185 (= H205)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
26% identity, 58% coverage: 25:249/386 of query aligns to 18:249/259 of 5zaiC
- active site: A65 (≠ G73), F70 (≠ L78), S82 (≠ N97), R86 (≠ A101), G110 (= G132), E113 (≠ G135), P132 (= P154), E133 (= E155), I138 (≠ L160), P140 (= P162), G141 (≠ D163), A226 (≠ L233), F236 (vs. gap)
- binding coenzyme a: K24 (≠ T31), L25 (= L32), A63 (= A71), G64 (= G72), A65 (≠ G73), D66 (= D74), I67 (≠ L75), P132 (= P154), R166 (≠ N187), F248 (= F248)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
27% identity, 63% coverage: 13:256/386 of query aligns to 37:290/290 of P14604
- E144 (≠ G135) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E155) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
27% identity, 63% coverage: 13:256/386 of query aligns to 5:258/258 of 1ey3A
- active site: A66 (≠ G73), M71 (≠ L78), S81 (≠ R109), L85 (= L113), G109 (= G132), E112 (≠ G135), P131 (= P154), E132 (= E155), T137 (≠ L160), P139 (= P162), G140 (≠ D163), K225 (≠ G223), F235 (≠ L233)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K30), L26 (= L32), A28 (= A34), A64 (= A71), G65 (= G72), A66 (≠ G73), D67 (= D74), I68 (≠ L75), L85 (= L113), W88 (vs. gap), G109 (= G132), P131 (= P154), L135 (≠ I158), G140 (≠ D163)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
27% identity, 63% coverage: 13:256/386 of query aligns to 7:260/260 of 1dubA
- active site: A68 (≠ G73), M73 (≠ L78), S83 (≠ R109), L87 (= L113), G111 (= G132), E114 (≠ G135), P133 (= P154), E134 (= E155), T139 (≠ L160), P141 (= P162), G142 (≠ D163), K227 (≠ G223), F237 (≠ L233)
- binding acetoacetyl-coenzyme a: K26 (= K30), A27 (≠ T31), L28 (= L32), A30 (= A34), A66 (= A71), A68 (≠ G73), D69 (= D74), I70 (≠ L75), Y107 (≠ I128), G110 (= G131), G111 (= G132), E114 (≠ G135), P133 (= P154), E134 (= E155), L137 (≠ I158), G142 (≠ D163), F233 (≠ L229), F249 (= F248)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
27% identity, 63% coverage: 13:256/386 of query aligns to 7:258/258 of 1mj3A
- active site: A68 (≠ G73), M73 (≠ L78), S83 (≠ E105), L85 (≠ E107), G109 (= G132), E112 (≠ G135), P131 (= P154), E132 (= E155), T137 (≠ L160), P139 (= P162), G140 (≠ D163), K225 (≠ G223), F235 (≠ L233)
- binding hexanoyl-coenzyme a: K26 (= K30), A27 (≠ T31), L28 (= L32), A30 (= A34), A66 (= A71), G67 (= G72), A68 (≠ G73), D69 (= D74), I70 (≠ L75), G109 (= G132), P131 (= P154), E132 (= E155), L135 (≠ I158), G140 (≠ D163)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
28% identity, 63% coverage: 13:256/386 of query aligns to 6:254/254 of 2dubA
- active site: A67 (≠ G73), M72 (≠ L78), S82 (≠ R109), G105 (= G132), E108 (≠ G135), P127 (= P154), E128 (= E155), T133 (≠ L160), P135 (= P162), G136 (≠ D163), K221 (≠ G223), F231 (≠ L233)
- binding octanoyl-coenzyme a: K25 (= K30), A26 (≠ T31), L27 (= L32), A29 (= A34), A65 (= A71), A67 (≠ G73), D68 (= D74), I69 (≠ L75), K70 (≠ Q76), G105 (= G132), E108 (≠ G135), P127 (= P154), E128 (= E155), G136 (≠ D163), A137 (≠ V164)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
28% identity, 62% coverage: 21:259/386 of query aligns to 17:260/260 of 2hw5C
- active site: A68 (≠ G73), M73 (≠ L78), S83 (≠ A100), L87 (≠ F104), G111 (= G132), E114 (≠ G135), P133 (= P154), E134 (= E155), T139 (≠ L160), P141 (= P162), G142 (≠ D163), K227 (≠ G223), F237 (≠ L233)
- binding crotonyl coenzyme a: K26 (= K30), A27 (≠ T31), L28 (= L32), A30 (= A34), K62 (= K67), I70 (≠ L75), F109 (≠ M130)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
26% identity, 49% coverage: 9:198/386 of query aligns to 1:170/250 of 3q0gD
- active site: A64 (≠ G73), M69 (≠ L78), T75 (≠ A100), F79 (= F104), G103 (= G132), E106 (≠ G135), P125 (= P154), E126 (= E155), V131 (≠ L160), P133 (= P162), G134 (≠ D163)
Sites not aligning to the query:
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
30% identity, 47% coverage: 21:200/386 of query aligns to 15:181/261 of 5jbxB
- active site: A67 (≠ G73), R72 (≠ H86), L84 (≠ F104), R88 (≠ Y108), G112 (= G132), E115 (≠ G135), T134 (≠ P154), E135 (= E155), I140 (≠ L160), P142 (= P162), G143 (≠ D163)
- binding coenzyme a: S24 (≠ K30), R25 (≠ T31), R26 (≠ L32), A28 (= A34), A65 (= A71), D68 (= D74), L69 (= L75), K70 (≠ Q76), L110 (≠ M130), G111 (= G131), T134 (≠ P154), E135 (= E155), L138 (≠ I158), R168 (≠ N187)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
26% identity, 49% coverage: 8:198/386 of query aligns to 1:175/255 of 3q0jC
- active site: A65 (≠ G73), M70 (≠ L78), T80 (≠ A100), F84 (= F104), G108 (= G132), E111 (≠ G135), P130 (= P154), E131 (= E155), V136 (≠ L160), P138 (= P162), G139 (≠ D163)
- binding acetoacetyl-coenzyme a: Q23 (≠ K30), A24 (≠ T31), L25 (= L32), A27 (= A34), A63 (= A71), G64 (= G72), A65 (≠ G73), D66 (= D74), I67 (≠ L75), K68 (≠ Q76), M70 (≠ L78), F84 (= F104), G107 (= G131), G108 (= G132), E111 (≠ G135), P130 (= P154), E131 (= E155), P138 (= P162), G139 (≠ D163), M140 (≠ V164)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
26% identity, 49% coverage: 8:198/386 of query aligns to 1:175/255 of 3q0gC
- active site: A65 (≠ G73), M70 (≠ L78), T80 (≠ A100), F84 (= F104), G108 (= G132), E111 (≠ G135), P130 (= P154), E131 (= E155), V136 (≠ L160), P138 (= P162), G139 (≠ D163)
- binding coenzyme a: L25 (= L32), A63 (= A71), I67 (≠ L75), K68 (≠ Q76), Y104 (≠ I128), P130 (= P154), E131 (= E155), L134 (≠ I158)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
26% identity, 49% coverage: 9:198/386 of query aligns to 1:174/256 of 3h81A
- active site: A64 (≠ G73), M69 (≠ L78), T79 (≠ A100), F83 (= F104), G107 (= G132), E110 (≠ G135), P129 (= P154), E130 (= E155), V135 (≠ L160), P137 (= P162), G138 (≠ D163)
Sites not aligning to the query:
6z1pBI mS93 (see paper)
22% identity, 46% coverage: 21:199/386 of query aligns to 32:201/1413 of 6z1pBI
- active site: T85 (≠ G73), S134 (≠ G132), E157 (= E155), D165 (= D163)
- binding : Y41 (≠ K30), K42 (≠ T31), Q43 (≠ L32), T45 (≠ A34), D47 (≠ S36), H49 (≠ E38), K83 (≠ A71), T85 (≠ G73), D86 (= D74), F87 (≠ L75), K88 (≠ Q76), K92 (≠ R80), L130 (≠ I128), K152 (≠ R150)
Sites not aligning to the query:
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
27% identity, 53% coverage: 30:235/386 of query aligns to 23:218/707 of 6yswA
- active site: A66 (≠ G73), I71 (≠ L78), A84 (≠ F104), Q88 (≠ Y108), G112 (= G132), E115 (≠ G135), P136 (= P154), E137 (= E155), G145 (≠ D163)
- binding coenzyme a: E23 (≠ K30), M25 (≠ L32), A66 (≠ G73), D67 (= D74), I68 (≠ L75), P136 (= P154), E137 (= E155), L140 (≠ I158)
Sites not aligning to the query:
6eqoA Tri-functional propionyl-coa synthase of erythrobacter sp. Nap1 with bound NADP+ and phosphomethylphosphonic acid adenylate ester (see paper)
26% identity, 48% coverage: 16:200/386 of query aligns to 862:1041/1804 of 6eqoA
Sites not aligning to the query:
- binding phosphomethylphosphonic acid adenylate ester: 456, 458, 535, 536, 537, 538, 558, 559, 560, 561, 562, 688, 714
- binding nadp nicotinamide-adenine-dinucleotide phosphate: 1261, 1265, 1379, 1400, 1403, 1404, 1405, 1424, 1425, 1429, 1444, 1492, 1493, 1497, 1514, 1517, 1713, 1730, 1731, 1774
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
27% identity, 37% coverage: 33:175/386 of query aligns to 25:144/692 of 6iunB
Sites not aligning to the query:
- active site: 248, 407, 428, 440, 478
- binding nicotinamide-adenine-dinucleotide: 300, 301, 302, 321, 322, 365, 377, 378, 380, 384, 388, 405, 407
1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
25% identity, 39% coverage: 51:200/386 of query aligns to 48:186/715 of 1wdlA
Sites not aligning to the query:
- active site: 430, 451, 463, 501
- binding nicotinamide-adenine-dinucleotide: 322, 324, 325, 344, 345, 400, 401, 403, 428, 429, 430
Query Sequence
>HSERO_RS01610 FitnessBrowser__HerbieS:HSERO_RS01610
MNQAAAPVVFEELTAANGRRIGIATLASEKTLHAISLEMVQLLTPQLQHWQADPEVAMVV
LQAQGEKAFCAGGDLQQLYRSMREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTKP
ILCWGHGIVMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKIGIF
LALTGANLNAQDALFAGLADYGMAHASKAAVFDALLSQPWGQGRDAELLDRVLAGAQAAS
RREGVAAFVPSNLRSHFDQIEALCRRPTLPEIVDAILAVQTDDAWLLKAQSTLRAGAPGS
AWLGYALQKKVRPLSLAEVFRLEFVVSLHCAARPDFVEGIRALIIEKDQKPQWKPASLKQ
ATPAWVEGFFADPWTQGGHPLADLGR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory