SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS03215 FitnessBrowser__HerbieS:HSERO_RS03215 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
25% identity, 28% coverage: 45:234/683 of query aligns to 24:204/643 of Q92911

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Q92911

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A102 (= A124) natural variant: A -> P

Sites not aligning to the query:

226 mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.
237 D→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
242 Required for homodimerization; Y→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
243 Required for homodimerization; T→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.
471 Required for homodimerization; Q→A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.
525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
536 T → Q: requires 2 nucleotide substitutions
556 S → Q: requires 2 nucleotide substitutions

P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
27% identity, 29% coverage: 37:237/683 of query aligns to 7:204/502 of P07117

query
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P07117

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Sites not aligning to the query:

257 R→C: Sodium-independent binding affinity for proline.
281 C→S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
344 C→S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
349 C→S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
376 mutation R->E,Q: No change in activity.; R→K: Loss of activity.

P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
21% identity, 18% coverage: 501:625/683 of query aligns to 374:501/662 of P11170

query
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P11170

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Q457 (≠ G581) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
T460 (≠ F584) mutation to W: Decreased affinity for glucose and phlorizin.

Sites not aligning to the query:

255 modified: Disulfide link with 608
608 modified: Disulfide link with 255

3dh4A Crystal structure of sodium/sugar symporter with bound galactose from vibrio parahaemolyticus (see paper)
26% identity, 17% coverage: 501:615/683 of query aligns to 311:427/512 of 3dh4A

query
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3dh4A

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binding beta-D-galactopyranose: Q393 (vs. gap)
binding sodium ion: A326 (= A516), S329 (= S519)

Sites not aligning to the query:

binding beta-D-galactopyranose: 40, 58, 59, 62, 225, 229, 259
binding sodium ion: 36

Query Sequence

>HSERO_RS03215 FitnessBrowser__HerbieS:HSERO_RS03215
MESNKDFFRRLCRYYGFYTLGFIAFLCVLAILEREGMPRTWIGYLFLFVTIGLYAGIGVV
CRTSDVPEYYVAGRRVPPLFNGMATAADWISAATFISLAGGLYLQGFDGLAYILGWTGGY
CLVALLIAPYLRKFGQYTIADFLAARYPGRPGSQGVNVIRILAVAATILISFTYVVAQIY
GVGLITSRFTGIDFSIGIFLGLASILVCSFLGGMRAITWTQVAQYIIILLAYLIPVIWLS
AKHTTVPVPPVAYGAVLPKLSAIEQRLEDDPKEKEIRKIFQARADDYERKLRNLPQSWVD
GRLEAHTNADQVRRDNHASLAEIKAASRALSSYPKSPEEAERKWTEARAANLARAQPPVS
QTEPFPGRDQESSDIKRNNFLALVFCLMLGTAALPHILMRYYTTPTVAGARTSVFWTLFF
IMLIYLTVPALTVLVKYDIYTSLVGSSYSHLPDWVYYWANMDKLNPLVSITDLNRDGLVQ
LGEISMDGDIIVLAMPEIAGLPYFVSGLVAAGGLAAALSTADGLLLTISNALSHDVYYKV
VDPSASTQKRVTISKLLLLVVALLAAYAASLKPGDILSMVGAAFSLAASTLFPALVLGVF
WKRANQAGAIAGILCGFLMCVYYMLHTNPSFGGSAAGQWFHIAPISAGIFGVPVGMAAMV
IVSLLTPPPDEATIALIDHIRTP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory