SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS05400 FitnessBrowser__HerbieS:HSERO_RS05400 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
24% identity, 73% coverage: 56:256/277 of query aligns to 56:263/281 of P94530

query
sites
P94530

K

K

S

V

F

M

S

S

L

F

R

D

W

N

Y

Y

E

T

N

F

L

L

F

F

S

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S

G

D

G

D

S

-

I

W

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K

A

W

A

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Q

F

N

N

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S

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L

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P

L

A

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A

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D

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D

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G

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A

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H

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A

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A

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P

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A

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D

G

G

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C

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K

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N

N

N

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F

A

A

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F

D180 (≠ L181) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
31% identity, 29% coverage: 147:227/277 of query aligns to 357:439/490 of 4ki0F

query
sites
4ki0F

L

L

I

I

L

I

A

V

H
x
N

A

T

A

W

L

L

G
|
G

A

Y

P

P

F
x
Y

V

M

V

M

T

I

T

L

V

C

L

M

A

G

T

L

L

L

Q

K

G

A

F

I

N

P

H

D

N

D

L

L

V

Y

R

E

A

A

S

S

L

A

S

M

L

D

G

G

A

A

S

G

P

P

L

F

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T

N

F

F

F

F

R

K

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T

L

L

P

P

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L

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L

A

I

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K

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L

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G

L

A

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L

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F

A

A
x
S

F

F

A

A

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x
N

F

F

D

N

E

N

V

F

V

V

L

L

T

I

-

Q

-

L

L

L

F

T

V

N

A

G

G

G

P

P

E

D

Q

R

binding alpha-D-glucopyranose: N361 (≠ H151), G365 (= G155), Y368 (≠ F158), S418 (≠ A208), N422 (≠ S212)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 310

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
31% identity, 29% coverage: 147:227/277 of query aligns to 372:454/514 of P02916

query
sites
P02916

L
|
L

I

I

L

I

A

V

H
x
N

A

T

A

W

L

L

G
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G

A

Y

P

P

F

Y

V

M

V

M

T

I

T

L

V

C

L

M

A

G

T

L

L

L

Q

K

G

A

F

I

N

P

H

D

N

D

L

L

V

Y

R
x
E

A

A

S
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S

L

A

S

M

L

D

G
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G

A

A

S

G

P

P

L

F

R

Q

T

N

F

F

F

F

R

K

V

I

T

T

L

L

P
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P

V

L

I

L

A

I

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L

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G

L

A

M

L

I

F

A

A

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F

F

A

A

T

F

S

N

F

F

D

N

E

N

V

F

V

V

L

L

T

I

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Q

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L

L

L

F

T

V

N

A

G

G

G

P

P

E

D

Q

R

L372 (= L147) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ H151) mutation N->K,H: No growth on maltose.
G380 (= G155) mutation G->C,S: No growth on maltose.
E401 (≠ R176) mutation E->A,C,K,L: Reduction of transport rate.
S403 (= S178) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G182) mutation G->A,P: No effect.
P420 (= P195) mutation to A: No effect.

Sites not aligning to the query:

334 L→W: Ability to transport lactose in a saturable manner.

Query Sequence

>HSERO_RS05400 FitnessBrowser__HerbieS:HSERO_RS05400
MNKPPLFPPYTSLVERAWFFASRGFNLLVLLFLVSPILVMIPLSFSDSSFLMYPIKSFSL
RWYENLFSSDDWVRAAQNSFIVAPAATVIATVLGTLAAVGLNKADFRGKGILMAILISPM
IVPVVVVGVGVYLFFARIGLSDSYLGLILAHAALGAPFVVTTVLATLQGFNHNLVRASLS
LGASPLRTFFRVTLPVIAPGLISGALFAFATSFDEVVLTLFVAGPEQATLPRQMFAGIKD
NISPTIAALATILIIFSTCLLLALEWLRGRNKAAARF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory