SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS05520 FitnessBrowser__HerbieS:HSERO_RS05520 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

5ze4A The structure of holo- structure of dhad complex with [2fe-2s] cluster (see paper)
30% identity, 89% coverage: 25:576/623 of query aligns to 17:537/569 of 5ze4A

query
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binding fe2/s2 (inorganic) cluster: N59 (≠ L80), C61 (≠ A82), S92 (≠ C114), D93 (= D115), A94 (≠ G116), C134 (= C156), D135 (= D157), A205 (≠ T222), C206 (= C223)
binding magnesium ion: D93 (= D115), D135 (= D157), E458 (= E495)

Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 94% coverage: 25:609/623 of query aligns to 56:606/608 of Q9LIR4

query
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C100 (≠ A82) binding
C173 (= C156) binding
D174 (= D157) binding
C245 (= C223) binding
E497 (= E495) binding

P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 83% coverage: 64:579/623 of query aligns to 45:547/575 of P9WKJ5

query
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P9WKJ5

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D

L

L

P

A

A

A

V

V

F

F

V

L

P

Y

A

A

G

G

P

S

M

I

T

L

S

P

G

G

M

R

S

A

N

K

-

L

S

S

D

D

-

G

-

S

-

E

-

R

K

D

V

V

K

T

I

I

R

I

Q

D

L

A

Y

F

T

E

A

A

-

V

-

G

-

A

-

C

-

S

-

R

G

G

K

L

I

M

G

S

R

R

A

A

E

D

L

V

L

D

E

A

A

I

E

E

S

R

K

A

S

I

Y

C

H

P

G

G

A

E

G

G

T

A

C
|
C

T

G

F

G

Y

M

G

Y

T

T

A

A

N

N

S

T

N

M

Q

A

M

S

L

A

M

A

E

E

A

A

M

L

G

G

L

M

H

S

L

L

P

P

G

G

A

S

A

A

F

A

I

P

T

P

P

A

N

T

T

D

P

R

L

R

R

R

D

D

A

G

L

F

T

-

A

-

A

-

A

A

A

R

K

R

Q

S

A

G

A

Q

K

A

I

V

T

V

D

E

L

L

G

L

T

R

Q

R

Y

G

T

I

P

T

V

A

G

R

R

D

M

I

I

L

D

T

E

K

K

E

S

A

I

F

V

E

N

N

A

A

I

I

V

A

A

V

L

V

H

M

A

A

T

F

G

G

G

G

S

S

T

T

N

N

H

A

T

V

L

L

H

H

L

L

V

L

A

A

I

I

A

A

K

H

A

E

A

A

G

N

I

V

V

A

I

L

D

S

W

L

D

Q

D

D

F

F

D

S

K

R

L

I

S

G

A

S

V

G

V

V

P

P

L

H

I

L

T

A

K

D

I

V

Y

K

P

P

N

F

G

G

-

R

-

H

-

V

T

M

A

S

D

D

V

V

N

D

H

H

F

I

H

-

A

-

A

-

G

G

G

G

T

V

G

P

F

V

V

V

L

M

R

K

E

A

L

L

I

L

D

D

A

A

G

G

L

L

M

L

H

H

D

G

D

D

V

C

T

L

T

T

I

V

L

T

G

G

Q

H

G

T

L

M

R

A

Q

E

H

N

C

-

K

-

E

-

P

-

M

-

L

L

A

A

A

A

E

-

G

-

E

-

N

-

G

-

R

-

P

-

G

-

I

-

V

I

T

T

W

P

H

P

D

D

A

P

P

-

A

-

Q

-

S

-

G

-

D

D

E

G

G

K

V

V

L

L

G

R

T

A

V

L

A

A

K

N

P

P

F

I

A

H

A

P

D

S

G

G

G

G

L

I

K

T

L

I

L

L

Q

H

G

G

N

S

L

L

G

A

R

P

-

E

-

G

A

A

V

V

I

V

K

K

I

T

S

A

A

G

V

F

K

D

P

S

E

D

H

-

R

-

V

V

V

F

E

E

A

G

P

T

A

A

V

R

V

V

F

F

H

D

S

G

Q

E

E

R

A

A

F

A

M

L

A

D

A

A

F

L

K

E

A

D

G

G

Q

T

L

I

D

T

R

V

D

G

F

D

V

A

A

V

V

V

I

I

T

R

F

Y

Q

E

G

G

P

P

R

K

A

G

N

G

-

P

G

G

M

M

P

R

E
|
E

L

M

H

L

A

A

L

I

T

T

P

G

A

A

L

I

-

K

-

G

-

A

G

G

I

L

L

-

Q

-

D

-

K

-

G

G

R

K

H

D

V

V

A

L

L

L

V

L

T

T

D

D

G

G

R

R

M

F

S
|
S

G

G

A

G

S

T

G

T

K

G

V

L

P

C

A

V

A

G

I

-

H

H

V

I

T

A

P

P

E

E

V

A

L

V

G

D

G

G

G

G

P

P

L

I

G

A

R

L

V

L

R

R

D

N

G

G

D

D

I

R

I

I

R

R

L

L

D

D

A

V

E

A

A

G

G

R

T

V

L

L

E

D

A

V

K

L

V

A

D

D

A

P

T

A

E

E

W

F

A

A

A

S

R

R

S

Q

Q

Q

D

D

S

F

S

S

C64 (≠ A82) binding
D96 (= D115) binding
C137 (= C156) binding
D138 (= D157) binding
K139 (= K158) binding via carbamate group; modified: N6-carboxylysine
C214 (= C223) binding
E465 (= E495) binding
S491 (= S522) mutation to A: Loss of catalytic activity.

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
31% identity, 83% coverage: 64:579/623 of query aligns to 32:534/562 of 6ovtA

query
sites
6ovtA

E

E

D

D

K

F

A

A

-

K

P

P

S

Q

L

I

A

G

I

V

V

A

S

S

A

S

Y

W

N

N

D

E

M

I

L

T

S

P

A
x
C

H

N

Q

L

P

S

F

L

E

D

R

R

F

L

P

A

Q

N

I

A

I

V

K

K

D

E

A

G

A

V

R

F

E

S

A

A

G

G

A

G

V

Y

A

P

-

L

Q

E

F

F

A

-

G

-

G

G

S

T

P

I

A

S

M

V

C

S

D
|
D

G
|
G

V

I

T

S

Q

M

G

G

Q

H

A

E

G

G

M

M

E

H

L

F

S

S

L

L

F

V

S

S

R

R

D

E

A

V

I

I

A

A

M

D

A

S

T

V

A

E

I

V

A

V

L

M

S

Q

H

A

N

E

M

R

F

L

D

D

A

G

A

S

L

V

Y

L

L

L

G

A

V

G

C
|
C

D
|
D

K
|
K

I

S

V

L

P

P

G

G

L

M

L

L

I

M

G

A

A

A

L

A

H

R

F

L

G

-

H

D

L

L

P

A

A

A

V

V

F

F

V

L

P

Y

A

A

G

G

P

S

M

I

T

L

S

P

G

G

M

R

S

A

N

K

-

L

S

S

D

D

-

G

-

S

-

E

-

R

K

D

V

V

K

T

I

I

R

I

Q

D

L

A

Y

F

T

E

A

A

-

V

-

G

-

A

-

C

-

S

-

R

G

G

K

L

I

M

G

S

R

R

A

A

E

D

L

V

L

D

E

A

A

I

E

E

S

R

K

A

S

I

Y

C

H

P

G

G

A

E

G

G

T
x
A

C
|
C

T

G

F

G

Y

M

G

Y

T

T

A

A

N

N

S

T

N

M

Q

A

M

S

L

A

M

A

E

E

A

A

M

L

G

G

L

M

H

S

L

L

P

P

G

G

A

S

A

A

F

A

I

P

T

P

P

A

N

T

T

D

P

R

L

R

R

R

D

D

A

G

L

F

T

-

A

-

A

-

A

A

A

R

K

R

Q

S

A

G

A

Q

K

A

I

V

T

V

D

E

L

L

G

L

T

R

Q

R

Y

G

T

I

P

T

V

A

G

R

R

D

M

I

I

L

D

T

E

K

K

E

S

A

I

F

V

E

N

N

A

A

I

I

V

A

A

V

L

V

H

M

A

A

T

F

G

G

G

G

S

S

T

T

N

N

H

A

T

V

L

L

H

H

L

L

V

L

A

A

I

I

A

A

K

H

A

E

A

A

G

N

I

V

V

A

I

L

D

S

W

L

D

Q

D

D

F

F

D

S

K

R

L

I

S

G

A

S

V

G

V

V

P

P

L

H

I

L

T

A

K

D

I

V

Y

K

P

P

N

F

G

G

-

R

-

H

-

V

T

M

A

S

D

D

V

V

N

D

H

H

F

I

H

-

A

-

A

-

G

G

G

G

T

V

G

P

F

V

V

V

L

M

R

K

E

A

L

L

I

L

D

D

A

A

G

G

L

L

M

L

H

H

D

G

D

D

V

C

T

L

T

T

I

V

L

T

G

G

Q

H

G

T

L

M

R

A

Q

E

H

N

C

-

K

-

E

-

P

-

M

-

L

L

A

A

A

A

E

-

G

-

E

-

N

-

G

-

R

-

P

-

G

-

I

-

V

I

T

T

W

P

H

P

D

D

A

P

P

-

A

-

Q

-

S

-

G

-

D

D

E

G

G

K

V

V

L

L

G

R

T

A

V

L

A

A

K

N

P

P

F

I

A

H

A

P

D

S

G

G

G

G

L

I

K

T

L

I

L

L

Q

H

G

G

N

S

L

L

G

A

R

P

-

E

-

G

A

A

V

V

I

V

K

K

I

T

S

A

A

G

V

F

K

D

P

S

E

D

H

-

R

-

V

V

V

F

E

E

A

G

P

T

A

A

V

R

V

V

F

F

H

D

S

G

Q

E

E

R

A

A

F

A

M

L

A

D

A

A

F

L

K

E

A

D

G

G

Q

T

L

I

D

T

R

V

D

G

F

D

V

A

A

V

V

V

I

I

T

R

F

Y

Q

E

G

G

P

P

R

K

A

G

N

G

-

P

G

G

M

M

P

R

E
|
E

L

M

H

L

A

A

L

I

T

T

P

G

A

A

L

I

-

K

-

G

-

A

G

G

I

L

L

-

Q

-

D

-

K

-

G

G

R

K

H

D

V

V

A

L

L

L

V

L

T

T

D

D

G

G

R

R

M

F

S

S

G

G

A

G

S

T

G

T

K

G

V

L

P

C

A

V

A

G

I

-

H

H

V

I

T

A

P

P

E

E

V

A

L

V

G

D

G

G

G

G

P

P

L

I

G

A

R

L

V

L

R

R

D

N

G

G

D

D

I

R

I

I

R

R

L

L

D

D

A

V

E

A

A

G

G

R

T

V

L

L

E

D

A

V

K

L

V

A

D

D

A

P

T

A

E

E

W

F

A

A

A

S

R

R

S

Q

Q

Q

D

D

S

F

S

S

binding fe2/s2 (inorganic) cluster: C51 (≠ A82), D83 (= D115), G84 (= G116), C124 (= C156), D125 (= D157), A200 (≠ T222), C201 (= C223)
binding magnesium ion: D83 (= D115), D125 (= D157), K126 (= K158), E452 (= E495)

Q1JUQ1 L-arabonate dehydratase; L-arabinonate dehydratase; EC 4.2.1.25 from Azospirillum brasilense (see paper)
30% identity, 82% coverage: 65:577/623 of query aligns to 39:532/583 of Q1JUQ1

query
sites
Q1JUQ1

D

D

K

G

A

R

P

P

S

I

L

I

A

G

I

I

V
x
C

S

N

A

T

Y

W

N

S

D

E

M

L

L

T

S

P

A
x
C

H

N

Q

A

P

H

F

F

E

R

R

K

F

L

P

A

Q

E

I

H

I

V

K

K

D

R

A

G

A

I

R

S

E

E

A

A

G

G

A

-

V

-

A

-

Q

-

F

-

A

-

G

-

G

G

S

F

P

P

A

V

M

E

C

F

D

P

G

V

V

F

T

S

Q

N

G

G

Q

E

A

S

G

N

M

L

E

R

L

P

S

S

-

A

L

M

F

L

S

T

R

R

D

N

A

L

I

A

A

S

M

M

A

D

T

V

A

E

I

E

A

A

L

I

S

R

H

G

N

N

M

P

F

I

D

D

A

A

A

V

L

V

Y

L

L

L

G

A

V

G

C
|
C

D

D

K

K

I

T

V

T

P

P

G

A

L

L

L

L

I

M

G

G

A

A

L

A

H

S

F

C

G

-

H

D

L

V

P

P

A

A

V

I

F

V

V

V

P

S

A

G

G

G

P

P

M

M

T

L

S

N

G

G

M

K

S

L

N

E

S

G

D

K

K

N

V

I

-

G

-

S

-

G

-

T

-

A

-

V

-

W

K

Q

I

L

R

H

Q

E

L

A

Y

L

T

K

A

A

G

G

K

E

I

I

G

D

R

V

A

H

E

H

L

F

L

L

E

S

A

A

E

E

S

A

K

G

S

M

Y

S

H

R

G

S

A

A

G

G

T

T

C
|
C

T

N

F

T

Y

M

G

G

T

T

A

A

N

S

S

T

N

M

Q

A

M

C

L

M

M

A

E

E

A

A

M

L

G

G

L

V

H

A

L

L

P

P

G

H

A

N

A

A

F

A

I

I

T

P

P

A

N

V

T

D

P

S

L

R

R

R

D

Y

A

V

L

L

T

-

A

-

A

-

A

A

A

H

K

M

Q

S

A

G

A

I

K

R

I

I

T

V

D

E

L

M

G

A

T

L

Q

E

Y

G

T

L

P

V

V

L

G

S

R

K

M

I

I

L

D

T

E

R

K

A

S

A

I

F

V

E

N

N

A

A

I

I

V

R

A

A

L

N

H

A

A

A

T

I

G

G

G

G

S

S

T

T

N

N

H

A

T

V

L

I

H

H

L

L

V

K

A

A

I

I

A

A

K

G

A

R

A

I

G

G

I

V

V

P

I

L

D

E

W

L

D

E

D

D

F

W

D

M

K

R

L

I

S

G

A

R

V

D

V

T

P

P

L

T

I

I

T

V

K

D

I

L

Y

M

P

P

N

S

G

G

T

R

A

F

D

P

V

M

N

E

H

E

F

F

H

Y

A

Y

A

A

G

G

G

G

T

L

G

P

F

A

V

V

L

L

R

R

E

R

L

L

I

G

D

E

A

G

G

G

L

L

M

L

H

P

D

N

-

P

D

D

V

A

T

L

T

T

I

V

L

N

G

G

Q

K

G

S

L

L

R

W

Q

D

H

N

C

V

K

R

E

E

P

-

M

-

L

-

A

-

A

-

E

-

G

-

E

-

N

-

G

-

R

-

P

-

G

-

I

-

V

-

T

-

W

-

H

-

D

-

A

-

P

-

A

A

Q

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N

G

Y

D

D

E

E

G

E

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I

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T

P

V

L

A

D

K

R

P

P

F

L

A

I

A

A

D

D

G

G

G

G

L

I

K

R

L

I

L

L

Q

R

G

G

N

N

L

L

G

A

R

P

-

R

-

G

A

A

V

V

I

L

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K

I

P

S

S

A

A

V

A

K

S

P

P

E

E

H

L

R

L

V

K

V

H

E

R

A

G

P

R

A

A

V

V

V

V

F

F

H

E

S

N

Q

L

E

D

A

H

F

Y

M

K

A

A

A

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F

I

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N

A

D

G

E

Q

A

L

L

D

D

R

I

D

D

F

A

V

S

A

S

V

V

I

M

T

V

F

L

Q

K

-

N

-
x
C

G

G

P

P

R

R

A

G

N

Y

-

P

G

G

M

M

P

A

E

E

L

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H

G

A

N

L

M

T

G

P

L

A

P

L

P

G

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I

L

L

L

Q

R

D

Q

K

G

G

V

R

K

H

D

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M

A

V

L

R

V

I

T

S

D

D

G

A

R

R

M

M

S

S

G

G

-

T

A

A

S

Y

G

G

K

T

V

V

P

-

A

-

A

V

I

L

H

H

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V

T

A

P

P

E

E

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A

L

A

G

A

G

G

G

G

P

P

L

L

G

A

R

A

V

V

R

R

D

N

G

G

D

D

I

W

I

I

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E

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L

D

D

A

C

E

E

A

A

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G

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T

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D

D

C47 (≠ V73) mutation to A: 2-fold reduction in activity and still binds a 4Fe-4S cluster.
C56 (≠ A82) mutation to A: Loss of activity but seems to be able to bind a Fe-S cluster.
C124 (= C156) binding ; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C197 (= C223) binding ; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C441 (vs. gap) mutation to A: Increase in activity and still binds a 4Fe-4S cluster.

8epzA Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mn
31% identity, 82% coverage: 65:576/623 of query aligns to 34:523/569 of 8epzA

query
sites
8epzA

D

D

K

G

A

R

P

P

S

V

L

I

A

G

I

I

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A

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M

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A
x
C

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F

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I

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D

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A

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E

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G

G

A

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-

A

-

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-

F

F

A

P

G

L

G

E

S

F

P

P

A

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M

M

C

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D

L

G

G

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x
E

T

T

Q

M

G

L

Q

R

A

P

G

-

M

-

E

-

L

T

S

A

L

M

F

L

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F

R

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D

N

A

L

I

A

A

S

M

M

A

D

T

V

A

E

I

E

A

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L

I

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H

G

N

N

M

P

F

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D

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A

G

A

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M

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G

C
|
C

D
|
D

K
|
K

I

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P

P

G

S

L

L

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M

I

M

G

G

A

A

L

A

H

S

F

C

G

-

H

D

L

L

P

P

A

T

V

I

F

G

V

V

P

S

A

G

G

G

P

P

M

M

T

L

S

S

G

G

M

K

-

F

-

R

-

G

-

R

-

E

-

L

-

G

S

S

N

G

S

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D

D

K

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x
W

K

K

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M

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Q

E

L

E

Y

V

T

R

A

A

G

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I

M

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Q

A

E

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F

L

F

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A

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E

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Y

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A

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x
H

C
|
C

T

M

F

T

Y

M

G

G

T

T

A

A

N

S

S

T

N

M

Q

A

M

S

L

M

M

V

E

E

A

A

M

L

G

G

L

M

H

S

L

L

P

P

G

G

A

N

A

A

F

A

I

I

T

P

P

A

N

V

T

D

P

A

L

R

R

R

D

N

A

L

L

L

T

A

A

R

A

A

A

S

A

G

K

R

Q

-

A

-

A

-

K

R

I

I

T

V

D

Q

L

M

G

V

T

K

Q

D

Y

D

T

L

P

V

V

M

G

S

R

K

M

I

I

L

D

T

E

R

K

Q

S

A

I

F

V

E

N

N

A

A

I

I

V

R

A

V

L

N

H

A

A

A

T

I

G

G

G

G

S

S

T

T

N

N

H

A

T

V

L

I

H

H

L

L

V

L

A

A

I

I

A

A

K

G

A

R

A

I

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I

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I

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L

D

A

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F

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K

A

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C

I

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I

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S

G

G

T

T

A

H

D

L

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M

N

E

H

D

F

F

H

Y

A

Y

A

A

G

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G

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T

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G

P

F

A

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D

D

A

-

G

-

L

V

M

I

H

A

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D

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L

T

T

I

V

L

N

G

G

Q

Q

G

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L

L

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Q

D

H

N

C

C

K

K

E

-

P

-

M

-

L

-

A

-

A

-

E

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G

-

E

-

N

-

G

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I

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W

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H

-

D

D

A

A

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P

A

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W

D

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R

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I

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A

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A

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P

F

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A

T

D

E

G

A

G

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L

I

K

A

L

V

L

L

Q

R

G

G

N

N

L

L

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C

R

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-

D

-

G

A

A

V

V

I

I

K

K

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A

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A

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A

H

L

R

L

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K

V

H

E

K

A

G

P

R

A

A

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F

F

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N

Q

S

E

E

A

H

F

M

M

H

A

E

A

R

F

M

K

D

A

D

G

E

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L

L

D

D

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D

D

F

E

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A

C

V

V

I

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V

F

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-

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-

C

G

G

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P

R

R

A

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Y

-

P

G

G

M

M

P

A

E
|
E

L

A

H

G

A

N

L

M

T

P

P

L

A

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L

P

G

K

I

I

L

L

Q

R

D

K

K

G

G

I

R

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D

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M

A

V

L

R

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V

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D

D

G

A

R

R

M

M

S
|
S

G

G

-

T

A

A

S

Y

G

G

K

T

V

V

P

-

A

-

A

V

I

L

H

H

V

V

T

A

P

P

E

E

V

A

L

A

G

A

G

G

G

G

P

P

L

L

G

A

R

L

V

V

R

Q

D

D

G

G

D

D

I

I

I

I

R

E

L

L

D

D

A

V

E

A

A

A

G

R

T

K

L

L

E

H

A

L

K

H

V

V

D

S

A

D

T

E

E

E

W

L

A

A

A

R

R

R

S

R

Q

E

binding bicarbonate ion: W162 (≠ V193), S470 (= S522)
binding fe2/s2 (inorganic) cluster: C51 (≠ A82), C119 (= C156), D120 (= D157), H191 (≠ T222), C192 (= C223)
binding manganese (ii) ion: E83 (≠ V117), D120 (= D157), K121 (= K158), E443 (= E495)

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
30% identity, 82% coverage: 65:574/623 of query aligns to 42:531/579 of B5ZZ34

query
sites
B5ZZ34

D

D

K

G

A

R

P

P

S

V

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A

G

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I

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A
x
C

H

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H

F

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R

E

F

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A

Q

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I

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K

D

A

A

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A

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E

E

A

A

G

G

A

-

V

-

A

-

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F

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-

G

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F

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P

A

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M

E

C

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D

P

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T

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Q

A

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Q
x
E

A

N

G

T

M

F

E

R

-

P

L

T

S

A

L

M

F

M

S

Y

R

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D

N

A

L

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A

A

A

M

L

A

A

T

V

A

E

I

E

A

A

L

I

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N

Q

M

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F

M

D

D

A

G

A

C

L

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Y

L

L

L

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V

V

G

C
|
C

D
|
D

K

K

I

T

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T

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P

G

S

L

L

L

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M

G

G

A

A

L

A

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C

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A

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I

F

V

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V

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T

A

G

G

G

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M

T

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N

G

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Y

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G

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H

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A

A

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A

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L

L

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E

A

A

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E

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A

K

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M

Y

S

H

R

G

S

A

S

G

G

T

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C
|
C

T

N

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M

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G

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T

A

A

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T

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Q

A

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M

A

E

E

A

A

M

L

G

G

L

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H

A

L

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A

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A

A

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A

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I

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-

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P

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D

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A

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L

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M

A

A

A

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A

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-

A

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M

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A

I

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E

N

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A

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A

T

L

N

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A

A

A

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I

G

G

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H

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-

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A

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A

A

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A

S

D

S

G

G

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G

G

N

N

L

L

G

A

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-

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A

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I

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S

A

A

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A

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S

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E

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L

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D

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I

E

D

A

D

F

Y

M

K

A

A

A

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N

A

D

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D

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N

L

L

D

D

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I

D

D

F

E

V

N

A
x
C

V

I

I

M

T

V

F

M

Q

K

-

N

-
x
C

G

G

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P

R

K

A

G

N

Y

-

P

G

G

M

M

P

A

E
|
E

L

V

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G

A

N

L

M

T

G

P

L

A

P

L

P

G

K

I

V

L

L

Q

K

D

K

K

G

G

I

R

L

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D

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M

A

V

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R

V

I

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S

D

D

G

A

R

R

M

M

S
|
S

G

G

-

T

A

A

S

Y

G

G

K

T

V

V

P

-

A

-

A

V

I

L

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H

V

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P

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E

E

V

A

L

A

G

V

G

G

G

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P

L

L

G

A

R

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V

V

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K

D

N

G

G

D

D

I

M

I

I

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E

L

L

D

D

A

V

E

P

A

N

G

R

T

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L

L

E

H

A

L

K

D

V

I

D

S

A

D

T

E

E

E

W

L

A

A

A

R

R

R

C59 (≠ A82) binding ; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
E91 (≠ Q121) binding
C127 (= C156) binding ; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
D128 (= D157) binding
C200 (= C223) binding ; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
C436 (≠ A481) mutation to S: No change in activity. Does not affect binding of iron-sulfur cluster.
C443 (vs. gap) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E453 (= E495) binding
S480 (= S522) mutation to A: Loss of activity.

5j85A Ser480ala mutant of l-arabinonate dehydratase (see paper)
29% identity, 82% coverage: 65:574/623 of query aligns to 39:528/576 of 5j85A

query
sites
5j85A

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binding fe2/s2 (inorganic) cluster: C56 (≠ A82), N89 (≠ A122), C124 (= C156), D125 (= D157), T196 (= T222), C197 (= C223)
binding magnesium ion: E88 (≠ Q121), D125 (= D157), K126 (= K158), T202 (= T228), E450 (= E495)

Q9A9Z2 D-xylonate dehydratase; XyDHT; Gluconate dehydratase; EC 4.2.1.82; EC 4.2.1.39 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see 2 papers)
28% identity, 82% coverage: 68:577/623 of query aligns to 50:548/595 of Q9A9Z2

query
sites
Q9A9Z2

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