SitesBLAST

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
49% identity, 100% coverage: 1:104/104 of query aligns to 1:104/104 of P00099

query
sites
P00099

M
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M

K
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K

Y
x
P

L
x
Y

L
x
A

L
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L

I
x
L

A
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S

T
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L

L
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L

V
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A

A
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T

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G

S
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T

A
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L

I
x
L

S
x
A

A
x
Q

E
x
G

A
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A

M
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W

A
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A

N
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E

A
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D

Q
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P

-
x
E

-
x
V

L
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L

A
x
F

K
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K

S
x
N

K
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K

N
x
G

C
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C

M
x
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A

C
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C

H
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H

S
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D

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M

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V

G
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G

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P

A
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A

F
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Y

K
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K

E
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D

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V

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A

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A

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K

Y
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F

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A

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G

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E

A
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E

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R

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I

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N
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N

G
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G

G
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S

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G
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G

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W
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W

G
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G

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M

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N
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N

K
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G

-

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-

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V

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S

E
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E

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A

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K

1:22 (vs. 1:22, 32% identical) signal peptide
23:104 (vs. 23:104, 54% identical) modified: mature protein, Cytochrome c-551
H38 (= H36) binding axial binding residue
M83 (= M81) binding axial binding residue

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
58% identity, 76% coverage: 26:104/104 of query aligns to 6:82/82 of 2exvA

query
sites
2exvA

L

L

A

A

K

K

S

N

K
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K

N

G

C
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C

M

V

A

A

C
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C

H
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H

S

A

V

I

D

D

A

T

K

K

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M

V

V

G
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G

P
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P

A

A

F
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Y

K

K

E

D

V
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V

A

A

K

A

K

K

Y

F

A

A

G

G

Q

Q

K

A

D

G

A

A

E

E

A

A

K

E

L
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L

T

A

Q

Q

K
x
R

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x
I

L

K

N

N

G

G

G
x
S

G
x
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G
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G

V
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V

W
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W

G
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G

A

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V
x
I

P
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P

M
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M

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N
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N

K

A

G

-

Q

-

V

V

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S

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D

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E

A

A

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L
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L

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K

K

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binding heme c: K10 (= K30), C12 (= C32), C15 (= C35), H16 (= H36), G24 (= G44), P25 (= P45), Y27 (≠ F47), V30 (= V50), L44 (= L64), R47 (≠ K67), I48 (≠ V68), S52 (≠ G72), Q53 (≠ G73), G54 (= G74), V55 (= V75), W56 (= W76), G57 (= G77), I59 (≠ V79), P60 (= P80), M61 (= M81), P62 (= P82), N64 (= N84), L74 (= L96)

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
57% identity, 76% coverage: 26:104/104 of query aligns to 6:82/82 of 351cA

query
sites
351cA

L

L

A

F

K

K

S

N

K

K

N

G

C
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C

M

V

A

A

C
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C

H
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H

S

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D

D

A

T

K

K

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V

G
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G

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P

A

A

F
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Y

K

K

E

D

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V

A

A

K

A

K

K

Y
x
F

A

A

G

G

Q

Q

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A

D

G

A

A

E

E

A

A

K

E

L
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L

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A

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Q

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R

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I

L

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N

N

G

G

G
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S

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G
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G

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V

W
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W

G
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G

A

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x
I

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M
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M

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P

N
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N

K

A

G

-

Q

-

V

V

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S

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A

D

E

E

A

A

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L
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L

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K

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K

binding protoporphyrin ix containing fe: C12 (= C32), C15 (= C35), H16 (= H36), V23 (= V43), G24 (= G44), P25 (= P45), Y27 (≠ F47), F34 (≠ Y54), L44 (= L64), R47 (≠ K67), I48 (≠ V68), S52 (≠ G72), Q53 (≠ G73), G54 (= G74), V55 (= V75), W56 (= W76), G57 (= G77), I59 (≠ V79), P60 (= P80), M61 (= M81), P62 (= P82), N64 (= N84), L74 (= L96)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
54% identity, 76% coverage: 26:104/104 of query aligns to 6:82/82 of 5xecA

query
sites
5xecA

L

L

A

F

K

K

S

N

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K

N
x
G

C
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C

M

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A

A

C
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C

H
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H

S

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D

D

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K

K

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K

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V

G
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G

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P

A

A

F
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Y

K

A

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D

V
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V

A

A

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K

Y
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Y

A

A

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G

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R

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K

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D

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A

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L
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G

G

G
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W
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W

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G

A

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V
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V

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M
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M

P
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P

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P

N
x
Q

K

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G

-

Q

N

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E

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L
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L

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I

L

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L

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K

binding heme c: K10 (= K30), G11 (≠ N31), C12 (= C32), C15 (= C35), H16 (= H36), V23 (= V43), G24 (= G44), P25 (= P45), Y27 (≠ F47), V30 (= V50), Y34 (= Y54), Y43 (≠ K63), L44 (= L64), K47 (= K67), I48 (≠ V68), G52 (= G72), S53 (≠ G73), G54 (= G74), V55 (= V75), W56 (= W76), G57 (= G77), V59 (= V79), P60 (= P80), M61 (= M81), P62 (= P82), Q64 (≠ N84), L74 (= L96), I78 (≠ V100)

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
52% identity, 79% coverage: 23:104/104 of query aligns to 1:81/81 of 1a56A

query
sites
1a56A

N

D

A

A

Q

D

L

L

A

A

K

K

S

K

K

N

N
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N

C
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C

M

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A

A

C
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C

H
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H

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E

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K

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V

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A

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L

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L
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N

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W

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M
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binding heme c: N9 (= N31), C10 (= C32), C13 (= C35), H14 (= H36), V21 (= V43), G22 (= G44), P23 (= P45), I28 (≠ V50), Y32 (= Y54), L42 (= L64), K45 (= K67), I46 (≠ V68), S50 (≠ G72), S51 (≠ G73), V53 (= V75), W54 (= W76), G55 (= G77), Q56 (≠ A78), I57 (≠ V79), M59 (= M81), N62 (= N84), V63 (≠ K85), N64 (≠ Q87)

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
55% identity, 79% coverage: 23:104/104 of query aligns to 1:83/83 of 5aurA

query
sites
5aurA

N

N

A

E

Q

Q

L

L

A

A

K

K

S

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K

K

N

G

C
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C

M

M

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A

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C

H
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H

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L

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A

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G

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A

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L
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W

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M
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M

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N
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L
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L

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W

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I

L

L

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L

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K

binding heme c: C10 (= C32), C13 (= C35), H14 (= H36), V24 (= V43), G25 (= G44), P26 (= P45), Y28 (≠ F47), V31 (= V50), Y35 (= Y54), Y44 (≠ K63), L45 (= L64), K48 (= K67), I49 (≠ V68), G53 (= G72), S54 (≠ G73), G55 (= G74), V56 (= V75), W57 (= W76), G58 (= G77), V60 (= V79), P61 (= P80), M62 (= M81), P63 (= P82), Q65 (≠ N84), L75 (= L96), I79 (≠ V100)

2d0sA Crystal structure of the cytochrome c552 from moderate thermophilic bacterium, hydrogenophilus thermoluteolus (see paper)
56% identity, 79% coverage: 23:104/104 of query aligns to 1:79/79 of 2d0sA

query
sites
2d0sA

N

D

A

E

Q

A

L

L

A

A

K

K

S

A

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K

N

G

C
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C

M

M

A

A

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C

H
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H

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D

D

A

K

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K

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G

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Y

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V

A

A

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K

Y
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Y

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E

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A

P

K

K

L
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L

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K

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V

L

K

N

K

G

G

G
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G

G
x
A

G
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G

V
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V

W
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W

G
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G

A

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V
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V

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M
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M

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A

P

N
x
H

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Q

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E

A

A

E

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A

I

R

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E

K

L

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K

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V

L

L

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T

L

L

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K

binding heme c: C10 (= C32), C13 (= C35), H14 (= H36), G22 (= G44), P23 (= P45), V28 (= V50), Y32 (= Y54), L40 (= L64), K43 (= K67), V44 (= V68), G48 (= G72), A49 (≠ G73), G50 (= G74), V51 (= V75), W52 (= W76), G53 (= G77), V55 (= V79), P56 (= P80), M57 (= M81), P58 (= P82), H60 (≠ N84)

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
58% identity, 76% coverage: 26:104/104 of query aligns to 6:82/82 of 1corA

query
sites
1corA

L

L

A

F

K

K

S

S

K
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K

N

P

C
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C

M

A

A

A

C
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C

H
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H

S

S

V

I

D

D

A

A

K

K

I

L

V
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V

G
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G

P
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P

A

A

F

F

K

K

E

E

V

V

A

A

K

A

K

K

Y
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Y

A

A

G

G

Q

Q

K

D

D

G

A

A

E

A

A

D

K

L

L
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L

T

A

Q

G

K
x
H

V
x
I

L

K

N

N

G

G

G
x
S

G
x
Q

G
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G

V
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V

W
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W

G
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G

A

P

V
x
I

P

P

M
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M

P

P

A

P

N
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N

K

P

G

-

Q

-

V

V

S

T

E

E

A

E

E

E

A

A

R

K

E

I

L
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L

V

A

K

E

W

W

V

I

L

L

T

S

L

Q

K

K

binding heme c: K10 (= K30), C12 (= C32), C15 (= C35), H16 (= H36), V23 (= V43), G24 (= G44), P25 (= P45), Y34 (= Y54), L44 (= L64), H47 (≠ K67), I48 (≠ V68), S52 (≠ G72), Q53 (≠ G73), G54 (= G74), V55 (= V75), W56 (= W76), G57 (= G77), I59 (≠ V79), M61 (= M81), N64 (= N84), L74 (= L96)

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
58% identity, 76% coverage: 26:104/104 of query aligns to 6:82/82 of 6kq1A

query
sites
6kq1A

L

L

A

F

K

K

S

S

K
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K

N
x
P

C
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C

M

A

A

A

C
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C

H
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H

S

S

V

I

D

D

A

A

K

K

I

M

V
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V

G
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G

P
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P

A

A

F

L

K

K

E

E

V
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V

A

A

K

A

K

K

Y
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Y

A

A

G

G

Q

Q

K

E

D

G

A

A

E

A

A

D

K

L

L
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L

T

A

Q

G

K
x
H

V
x
I

L

K

N

N

G

G

G
x
T

G
x
Q

G
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G

V
x
N

W
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W

G
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G

A

P

V
x
I

P
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P

M
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M

P

P

A

P

N
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N

K

P

G

-

Q

-

V

V

S

T

E

E

A

E

E
|
E

A

A

R

K

E

T

L
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L

V

A

K

E

W

W

V

V

L

L

T

S

L

L

K

K

binding heme c: P11 (≠ N31), C12 (= C32), C15 (= C35), H16 (= H36), V23 (= V43), G24 (= G44), P25 (= P45), V30 (= V50), Y34 (= Y54), L44 (= L64), H47 (≠ K67), I48 (≠ V68), T52 (≠ G72), Q53 (≠ G73), G54 (= G74), N55 (≠ V75), W56 (= W76), G57 (= G77), I59 (≠ V79), P60 (= P80), M61 (= M81), N64 (= N84), L74 (= L96)
binding zinc ion: K10 (= K30), E70 (= E92)

Sites not aligning to the query:

binding zinc ion: 2, 4

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
57% identity, 76% coverage: 26:104/104 of query aligns to 6:82/82 of 1cchA

query
sites
1cchA

L

L

A

F

K

K

S

S

K

K

N

P

C
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C

M

A

A

A

C
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C

H
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H

S

S

V

V

D

D

A

T

K

K

I

M

V

V

G
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G

P
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P

A

A

F

L

K

K

E

E

V

V

A

A

K

A

K

K

Y

N

A

A

G

G

Q

V

K

E

D

G

A

A

E

A

A

D

K

T

L
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L

T

A

Q

L

K
x
H

V
x
I

L

K

N

N

G

G

G
x
S

G
x
Q

G
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G

V
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V

W
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W

G
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G

A

P

V
x
I

P

P

M
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M

P
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P

A

P

N
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N

K

P

G

-

Q

-

V
|
V

S

T

E

E

A

E

E

E

A

A

R

K

E

I

L

L

V

A

K

E

W

W

V

V

L

L

T

S

L

L

K

K

binding heme c: C12 (= C32), C15 (= C35), H16 (= H36), G24 (= G44), P25 (= P45), L44 (= L64), H47 (≠ K67), I48 (≠ V68), S52 (≠ G72), Q53 (≠ G73), G54 (= G74), V55 (= V75), W56 (= W76), G57 (= G77), I59 (≠ V79), M61 (= M81), P62 (= P82), N64 (= N84), V66 (= V88)

4xxlA Crystal structure of class 1 cytochrome mtod from sideroxydans lithotrophicus es-1 (see paper)
40% identity, 75% coverage: 26:103/104 of query aligns to 10:90/92 of 4xxlA

query
sites
4xxlA

L

L

A

A

K

R

S

E

K
x
N

N
|
N

C
|
C

M

F

A

K

C
|
C

H
|
H

S

G

V

V

D

D

A

K

K

E

I

K

V
x
D

G
|
G

P
|
P

A

S

F
x
Y

K

K

E

K

V
|
V

A

A

K

E

K
|
K

Y
|
Y

A

R

G

G

Q

K

K

A

D

D

A

A

E

E

A

A

K

K

L
|
L

T

I

Q

H

K
x
H

V
|
V

L

T

N

S

G

G

G

E

G

K

G
x
A

V
x
K

W
x
F

G

P

A

D

V

G

P

H

M

E

P

E

A

E

N
x
H

K
|
K

-

N

-

I

-

N

G

G

Q

K

V

A

S

S

E

P

A

E

E

A

A

I

R

K

E

N

L

L

V

V

K

D

W

W

V

I

L

L

T

S

L

L

binding heme c: N14 (≠ K30), N15 (= N31), C16 (= C32), C19 (= C35), H20 (= H36), D27 (≠ V43), G28 (= G44), P29 (= P45), Y31 (≠ F47), V34 (= V50), K37 (= K53), Y38 (= Y54), L48 (= L64), H51 (≠ K67), V52 (= V68), A58 (≠ G74), K59 (≠ V75), F60 (≠ W76), H68 (≠ N84), K69 (= K85)

Query Sequence

>HSERO_RS05540 FitnessBrowser__HerbieS:HSERO_RS05540
MKYLLLIATLVAGSAISAEAMANAQLAKSKNCMACHSVDAKIVGPAFKEVAKKYAGQKDA
EAKLTQKVLNGGGGVWGAVPMPANKGQVSEAEARELVKWVLTLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory