SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS07200 FitnessBrowser__HerbieS:HSERO_RS07200 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

Q02I31 Putative pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
35% identity, 90% coverage: 6:111/118 of query aligns to 2:110/118 of Q02I31

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T2 (≠ S6) modified: Phosphothreonine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2v6tB Crystal structure of a complex of pterin-4a-carbinolamine dehydratase from toxoplasma gondii with 7,8-dihydrobiopterin (see paper)
31% identity, 67% coverage: 31:109/118 of query aligns to 17:93/100 of 2v6tB

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active site: D54 (≠ H66), H58 (= H70), H59 (= H71), H76 (= H88), D77 (≠ S89), D85 (= D101)
binding 2-amino-6-(1,2-dihydroxy-propyl)-7,8-dihydro-6h-pteridin-4-one: D57 (= D69), H58 (= H70), H59 (= H71), D77 (≠ S89)

Query Sequence

>HSERO_RS07200 FitnessBrowser__HerbieS:HSERO_RS07200
MSITASTLAAAHCIQPVDALDDSAITAHLAVLPDWQIEAGKLVRSFAFNNYYETLAFVNA
IAWMIHAEDHHPELIVSYNRCTVKFDTHSVNAGRGGLSANDFVCAAKVDALFAQRAHA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory