SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS07255 FitnessBrowser__HerbieS:HSERO_RS07255 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6wywA Crystal structure of pseudomonas 7a glutaminase-asparaginase in complex with l-asp at ph 4.5
63% identity, 92% coverage: 31:356/356 of query aligns to 3:317/317 of 6wywA

query
sites
6wywA

N

N

V

V

V

V

V

I

I

L

G

A

T

T

G

G

G
|
G

T
|
T

I

I

A

A

G

G

A

A

G

-

A

-

S

-

S

-

V

-

N

-

T

-

A

-

A

-

Y

-

Q

-

S

-

A

A

V

K

V

L

P

G

V

V

D

D

K

K

I

L

I

I

A

A

A

G

V

V

P

P

E

E

I

L

S

A

K

D

I

I

A

A

N

N

V

V

R

R

G

G

E

E

Q

Q

I

V

F

M

Q

Q

I

I

G
x
A

S
|
S

E
|
E

S

S

F

I

N

S

D

N

E

D

R

D

L

L

I

L

K

K

L

L

G

G

K

K

R

R

V

V

S

A

E

E

L

L

L

A

K

E

Q

S

N

K

D

D

V

V

D

D

G

G

I

I

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

L

E

E

E

E

T

T

A

A

Y

F

F

F

L

L

N

N

L

L

V

V

L

E

K

K

S

T

D

D

K

K

P

P

V

I

V

V

V

V

V

V

G

G

S

S

M

M

R

R

P

P

G

G

T

T

A

A

L

M

G

S

A

A

D

D

G

G

A

M

L

L

N

N

L

L

Y

Y

D

N

A

A

V

V

L

A

V

V

A

A

G

S

S

D

K

K

E

Q

A

S

A

R

G

G

K

K

G

G

T

V

L

L

V

V

V

T

M

M

N

N

D

D

E

E

I

I

H

Q

S

S

G

G

R

R

D

D

V

V

T

S

K
|
K

S

A

N

V

T

N

F

I

K

K

V

T

E

E

T

A

F

F

R

K

S

S

P

A

Y

W

G

G

P

P

L

M

G

G

Y

M

V

V

V

V

E

E

N

G

K

K

L

S

S

Y

F

W

Y

F

R

R

L

L

P

P

A

A

R

K

P

R

H

H

T

T

T

V

Q

N

T

S

E

E

F

F

D

D

I

I

D

K

K

Q

I

I

S

S

S

S

L

L

P

P

R

Q

V

V

D

D

I

I

V

A

Y

Y

N

G

Y

Y

G

G

N

N

V

V

S

T

R

D

T

T

A

A

Y

Y

D

K

A

A

F

L

V

A

A

Q

A

N

G

G

A

A

K

K

A

A

I

L

I

I

H

H

D

A

G

G

T

T

G

G

N

N

G

G

S

S

V

V

A

S

E

S

Q

R

I

V

V

V

P

P

V

A

L

L

Q

Q

E

E

V

L

R

R

S

K

K

N

G

G

V

V

Q

Q

V

I

I

I

R

R

A

S

S

S

R

H

T

V

G

N

S

Q

-

G

G

G

V

F

V

V

I

L

R

R

N

N

G

A

E
|
E

Q

Q

P

P

D

D

D

D

K

K

F

N

D

D

W

W

V

V

V

V

T

A

D

H

D

D

Q

L

N

N

A

P

Q

Q

K

K

A

A

R

R

I

I

L

L

A

A

E

M

L

V

A

A

L

M

T

T

K

K

T

T

N

Q

D

D

P

S

K

K

E

E

L

L

Q

Q

K

R

I

I

M

F

W

W

K

E

Y

Y

active site: T12 (= T40), T80 (= T120), D81 (= D121), K153 (= K193), E274 (= E313)
binding aspartic acid: G11 (= G39), T12 (= T40), A46 (≠ G86), S47 (= S87), E48 (= E88), G79 (= G119), T80 (= T120), D81 (= D121)

6wyyA Crystal structure of pseudomonas 7a glutaminase-asparaginase in complex with l-glu at ph 6.5
62% identity, 92% coverage: 28:356/356 of query aligns to 1:315/315 of 6wyyA

query
sites
6wyyA

K

K

K

L

P

A

N

N

V

V

V

V

V

I

I

L

G

A

T

T

G

G

G
|
G

T
|
T

I

I

A

A

G

G

A

L

G

G

A

-

S

-

S

-

V

-

N

-

T

-

A

-

A

-

Y

-

Q

-

S

-

A

-

V

-

V

-

P

-

V

V

D

D

K

K

I

L

I

I

A

A

A

G

V

V

P

P

E

E

I

L

S

A

K

D

I

I

A

A

N

N

V

V

R

R

G

G

E

E

Q

Q

I

V

F

M

Q

Q

I

I

G

A

S
|
S

E
|
E

S

S

F

I

N

S

D

N

E

D

R

D

L

L

I

L

K

K

L

L

G

G

K

K

R

R

V

V

S

A

E

E

L

L

L

A

K

E

Q

S

N

K

D

D

V

V

D

D

G

G

I

I

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

L

E

E

E

E

T

T

A

A

Y

F

F

F

L

L

N

N

L

L

V

V

L

E

K

K

S

T

D

D

K

K

P

P

V

I

V

V

V

V

V

V

G

G

S

S

M

M

R

R

P

P

G

G

T

T

A

A

L

M

G

S

A

A

D

D

G

G

A

M

L

L

N

N

L

L

Y

Y

D

N

A

A

V

V

L

A

V

V

A

A

G

S

S

D

K

K

E

Q

A

S

A

R

G

G

K

K

G

G

T

V

L

L

V

V

V

T

M

M

N

N

D

D

E

E

I

I

H

Q

S

S

G

G

R

R

D

D

V

V

T

S

K
|
K

S

A

N

V

T

N

F

I

K

K

V

T

E

E

T

A

F

F

R

K

S

S

P

A

Y

W

G

G

P

P

L

M

G

G

Y

M

V

V

V

V

E

E

N

G

K

K

L

S

S

Y

F

W

Y

F

R

R

L

L

P

P

A

A

R

K

P

R

H

H

T

T

T

V

Q

N

T

S

E

E

F

F

D

D

I

I

D

K

K

Q

I

I

S

S

S

S

L

L

P

P

R

Q

V

V

D

D

I

I

V

A

Y

Y

N

G

Y

Y

G

G

N

N

V

V

S

T

R

D

T

T

A

A

Y

Y

D

K

A

A

F

L

V

A

A

Q

A

N

G

G

A

A

K

K

A

A

I

L

I

I

H

H

D

A

G

G

T

T

G

G

N

N

G

G

S

S

V

V

A

S

E

S

Q

R

I

V

V

V

P

P

V

A

L

L

Q

Q

E

E

V

L

R

R

S

K

K

N

G

G

V

V

Q

Q

V

I

I

I

R

R

A

S

S

S

R

H

T

V

G

N

S

Q

-

G

G

G

V

F

V

V

I

L

R

R

N

N

G

A

E
|
E

Q

Q

P

P

D

D

D

D

K

K

F

N

D

D

W

W

V

V

V

V

T

A

D

H

D

D

Q

L

N

N

A

P

Q

Q

K

K

A

A

R

R

I

I

L

L

A

A

E

M

L

V

A

A

L

M

T

T

K

K

T

T

N

Q

D

D

P

S

K

K

E

E

L

L

Q

Q

K

R

I

I

M

F

W

W

K

E

Y

Y

active site: T13 (= T40), T78 (= T120), D79 (= D121), K151 (= K193), E272 (= E313)
binding glutamic acid: G12 (= G39), T13 (= T40), S45 (= S87), E46 (= E88), G77 (= G119), T78 (= T120), D79 (= D121)

4pgaA Glutaminase-asparaginase from pseudomonas 7a (see paper)
65% identity, 92% coverage: 28:356/356 of query aligns to 1:330/330 of 4pgaA

query
sites
4pgaA

K

K

K

L

P

A

N

N

V

V

V

V

V

I

I

L

G

A

T

T

G

G

G
|
G

T
|
T

I

I

A

A

G

G

A

A

G

G

A

A

S

S

S

A

V

A

N

N

T

S

A

A

A

T

Y
|
Y

Q

Q

S

A

A

A

V

K

V

V

P

G

V

V

D

D

K

K

I

L

I

I

A

A

A

G

V

V

P

P

E

E

I

L

S

A

K

D

I

L

A

A

N

N

V

V

R

R

G

G

E

E

Q

Q

I

V

F

M

Q

Q

I

I

G

A

S
|
S

E
|
E

S

S

F

I

N

T

D

N

E

D

R

D

L

L

I

L

K

K

L

L

G

G

K

K

R

R

V

V

S

A

E

E

L

L

L

A

K

D

Q

S

N

N

D

D

V

V

D

D

G

G

I

I

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

L

E

E

E

E

T

T

A

A

Y

Y

F

F

L

L

N

N

L

L

V

V

L

Q

K

K

S

T

D

D

K

K

P

P

V

I

V

V

V

V

V

V

G

G

S

S

M

M

R

R

P

P

G

G

T

T

A

A

L

M

G

S

A

A

D

D

G

G

A

M

L

L

N

N

L

L

Y

Y

D

N

A

A

V

V

L

A

V

V

A

A

G

S

S

N

K

K

E

D

A

S

A

R

G

G

K

K

G

G

T

V

L

L

V

V

V

T

M

M

N

N

D

D

E

E

I

I

H

Q

S

S

G

G

R

R

D

D

V

V

T

S

K
|
K

S

S

N

I

T

N

F

I

K

K

V

T

E

E

T

A

F

F

R

K

S

S

P

A

Y

W

G

G

P

P

L

L

G

G

Y

M

V

V

V

V

E

E

N

G

K

K

L

S

S

Y

F

W

Y

F

R

R

L

L

P

P

A

A

R

K

P

R

H

H

T

T

T

V

Q

N

T

S

E

E

F

F

D

D

I

I

D

K

K

Q

I

I

S

S

S

S

L

L

P

P

R

Q

V

V

D

D

I

I

V

A

Y

Y

N

S

Y

Y

G

G

N

N

V

V

S

T

R

D

T

T

A

A

Y

Y

D

K

A

A

F

L

V

A

A

Q

A

N

G

G

A

A

K

K

A

A

I

L

I

I

H

H

D

A

G

G

T

T

G

G

N

N

G

G

S

S

V

V

A

S

E

S

Q

R

I

V

V

V

P

P

V

A

L

L

Q

Q

E

Q

V

L

R

R

S

K

K

N

G

G

V

T

Q

Q

V

I

I

I

R

R

A

S

S

S

R

H

T

V

G

N

S

Q

-

G

G

G

V

F

V

V

I

L

R

R

N

N

G

A

E
|
E

Q

Q

P

P

D

D

D

D

K

K

F

N

D

D

W

W

V

V

V

V

T

A

D

H

D

D

Q

L

N

N

A

P

Q

E

K

K

A

A

R

R

I

I

L

L

A

A

E

M

L

V

A

A

L

M

T

T

K

K

T

T

N

Q

D

D

P

S

K

K

E

E

L

L

Q

Q

K

R

I

I

M

F

W

W

K

E

Y

Y

active site: T13 (= T40), Y27 (= Y54), T93 (= T120), D94 (= D121), K166 (= K193), E287 (= E313)
binding ammonium ion: E61 (= E88), D94 (= D121)
binding sulfate ion: G12 (= G39), T13 (= T40), S60 (= S87), G92 (= G119), T93 (= T120), D94 (= D121)

1djpA Crystal structure of pseudomonas 7a glutaminase-asparaginase with the inhibitor don covalently bound in the active site (see paper)
65% identity, 92% coverage: 28:356/356 of query aligns to 1:330/330 of 1djpA

query
sites
1djpA

K

K

K

L

P

A

N

N

V

V

V

V

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

A

G

G

A

A

S

S

S

A

V

A

N

N

T

S

A

A

A

T

Y
|
Y

Q

Q

S

A

A

A

V

K

V

V

P

G

V

V

D

D

K

K

I

L

I

I

A

A

A

G

V

V

P

P

E

E

I

L

S

A

K

D

I

L

A

A

N

N

V

V

R

R

G

G

E

E

Q

Q

I

V

F

M

Q

Q

I

I

G

A

S
|
S

E
|
E

S

S

F

I

N

T

D

N

E

D

R

D

L

L

I

L

K

K

L

L

G

G

K

K

R

R

V

V

S

A

E

E

L

L

L

A

K

D

Q

S

N

N

D

D

V

V

D

D

G

G

I

I

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

L

E

E

E

E

T

T

A

A

Y

Y

F

F

L

L

N

N

L

L

V

V

L

Q

K

K

S

T

D

D

K

K

P

P

V

I

V

V

V

V

V

V

G

G

S
|
S

M

M

R

R

P

P

G

G

T

T

A

A

L

M

G

S

A

A

D

D

G

G

A

M

L

L

N

N

L

L

Y

Y

D

N

A

A

V

V

L

A

V

V

A

A

G

S

S

N

K

K

E

D

A

S

A

R

G

G

K

K

G

G

T

V

L

L

V

V

V

T

M

M

N

N

D

D

E

E

I

I

H

Q

S

S

G

G

R

R

D

D

V

V

T

S

K
|
K

S

S

N

I

T

N

F

I

K

K

V

T

E

E

T

A

F

F

R

K

S

S

P

A

Y

W

G

G

P

P

L

L

G

G

Y

M

V

V

V

V

E

E

N

G

K

K

L

S

S

Y

F

W

Y

F

R

R

L

L

P

P

A

A

R

K

P

R

H

H

T

T

T

V

Q

N

T

S

E

E

F

F

D

D

I

I

D

K

K

Q

I

I

S

S

S

S

L

L

P

P

R

Q

V

V

D

D

I

I

V

A

Y

Y

N

S

Y

Y

G

G

N

N

V

V

S

T

R

D

T

T

A

A

Y

Y

D

K

A

A

F

L

V

A

A

Q

A

N

G

G

A

A

K

K

A

A

I

L

I

I

H

H

D

A

G

G

T

T

G

G

N

N

G

G

S

S

V

V

A

S

E

S

Q

R

I

V

V

V

P

P

V

A

L

L

Q

Q

E

Q

V

L

R

R

S

K

K

N

G

G

V

T

Q

Q

V

I

I

I

R

R

A

S

S

S

R

H

T

V

G

N

S

Q

-

G

G

G

V

F

V

V

I

L

R

R

N

N

G

A

E
|
E

Q

Q

P

P

D

D

D

D

K

K

F

N

D

D

W

W

V

V

V

V

T

A

D

H

D

D

Q

L

N

N

A

P

Q

E

K

K

A

A

R

R

I

I

L

L

A

A

E

M

L

V

A

A

L

M

T

T

K

K

T

T

N

Q

D

D

P

S

K

K

E

E

L

L

Q

Q

K

R

I

I

M

F

W

W

K

E

Y

Y

active site: T13 (= T40), Y27 (= Y54), T93 (= T120), D94 (= D121), K166 (= K193), E287 (= E313)
binding 5,5-dihydroxy-6-oxo-L-norleucine: T13 (= T40), Y27 (= Y54), S60 (= S87), E61 (= E88), G92 (= G119), T93 (= T120), D94 (= D121), S118 (= S145)

1djoA Crystal structure of pseudomonas 7a glutaminase-asparaginase with the inhibitor donv covalently bound in the active site (see paper)
65% identity, 92% coverage: 28:356/356 of query aligns to 1:330/330 of 1djoA

query
sites
1djoA

K

K

K

L

P

A

N

N

V

V

V

V

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

A

G

G

A

A

S

S

S

A

V

A

N

N

T

S

A

A

A

T

Y
|
Y

Q

Q

S

A

A

A

V

K

V

V

P

G

V

V

D

D

K

K

I

L

I

I

A

A

A

G

V

V

P

P

E

E

I

L

S

A

K

D

I

L

A

A

N

N

V

V

R

R

G

G

E

E

Q

Q

I

V

F

M

Q

Q

I

I

G
x
A

S
|
S

E
|
E

S

S

F

I

N

T

D

N

E

D

R

D

L

L

I

L

K

K

L

L

G

G

K

K

R

R

V

V

S

A

E

E

L

L

L

A

K

D

Q

S

N

N

D

D

V

V

D

D

G

G

I

I

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

L

E

E

E

E

T

T

A

A

Y

Y

F

F

L

L

N

N

L

L

V

V

L

Q

K

K

S

T

D

D

K

K

P

P

V

I

V

V

V

V

V

V

G

G

S

S

M

M

R

R

P

P

G

G

T

T

A

A

L

M

G

S

A

A

D

D

G

G

A

M

L

L

N

N

L

L

Y

Y

D

N

A

A

V

V

L

A

V

V

A

A

G

S

S

N

K

K

E

D

A

S

A

R

G

G

K

K

G

G

T

V

L

L

V

V

V

T

M

M

N

N

D

D

E

E

I

I

H

Q

S

S

G

G

R

R

D

D

V

V

T

S

K
|
K

S

S

N

I

T

N

F

I

K

K

V

T

E

E

T

A

F

F

R

K

S

S

P

A

Y

W

G

G

P

P

L

L

G

G

Y

M

V

V

V

V

E

E

N

G

K

K

L

S

S

Y

F

W

Y

F

R

R

L

L

P

P

A

A

R

K

P

R

H

H

T

T

T

V

Q

N

T

S

E

E

F

F

D

D

I

I

D

K

K

Q

I

I

S

S

S

S

L

L

P

P

R

Q

V

V

D

D

I

I

V

A

Y

Y

N

S

Y

Y

G

G

N

N

V

V

S

T

R

D

T

T

A

A

Y

Y

D

K

A

A

F

L

V

A

A

Q

A

N

G

G

A

A

K

K

A

A

I

L

I

I

H

H

D

A

G

G

T

T

G

G

N

N

G

G

S

S

V

V

A

S

E

S

Q

R

I

V

V

V

P

P

V

A

L

L

Q

Q

E

Q

V

L

R

R

S

K

K

N

G

G

V

T

Q

Q

V

I

I

I

R

R

A

S

S

S

R

H

T

V

G

N

S

Q

-

G

G

G

V

F

V

V

I

L

R

R

N

N

G

A

E
|
E

Q

Q

P

P

D

D

D

D

K

K

F

N

D

D

W

W

V

V

V

V

T

A

D

H

D

D

Q

L

N

N

A

P

Q

E

K

K

A

A

R

R

I

I

L

L

A

A

E

M

L

V

A

A

L

M

T

T

K

K

T

T

N

Q

D

D

P

S

K

K

E

E

L

L

Q

Q

K

R

I

I

M

F

W

W

K

E

Y

Y

active site: T13 (= T40), Y27 (= Y54), T93 (= T120), D94 (= D121), K166 (= K193), E287 (= E313)
binding 4,4-dihydroxy-5-oxo-L-norvaline: T13 (= T40), Y27 (= Y54), A59 (≠ G86), S60 (= S87), E61 (= E88), G92 (= G119), T93 (= T120), D94 (= D121)

6wyzA Crystal structure of pseudomonas 7a glutaminase-asparaginase (mutant k173m) in complex with d-glu at ph 5.5
62% identity, 92% coverage: 31:356/356 of query aligns to 3:312/312 of 6wyzA

query
sites
6wyzA

N

N

V

V

V

V

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

-

G

-

A

-

S

-

S

-

V

-

N

-

T

-

A

-

A

-

Y

-

Q

-

S

-

A

-

V

-

V

-

P

-

V

V

D

D

K

K

I

L

I

I

A

A

A

G

V

V

P

P

E

E

I

L

S

A

K

D

I

I

A

A

N

N

V

V

R

R

G

G

E

E

Q

Q

I

V

F

M

Q

Q

I

I

G
x
A

S
|
S

E
|
E

S

S

F

I

N

S

D

N

E

D

R

D

L

L

I

L

K

K

L

L

G

G

K

K

R

R

V

V

S

A

E

E

L

L

L

A

K

E

Q

S

N

K

D

D

V

V

D

D

G

G

I

I

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

L

E

E

E

E

T

T

A

A

Y

F

F

F

L

L

N

N

L

L

V

V

L

E

K

K

S

T

D

D

K

K

P

P

V

I

V

V

V

V

V

V

G

G

S
|
S

M

M

R

R

P

P

G

G

T

T

A

A

L

M

G

S

A

A

D

D

G

G

A

M

L

L

N

N

L

L

Y

Y

D

N

A

A

V

V

L

A

V

V

A

A

G

S

S

D

K

K

E

Q

A

S

A

R

G

G

K

K

G

G

T

V

L

L

V

V

V

T

M

M

N

N

D

D

E

E

I

I

H

Q

S

S

G

G

R

R

D

D

V

V

T

S

K
x
M

S

A

N

V

T

N

F

I

K

K

V

T

E

E

T

A

F

F

R

K

S

S

P

A

Y

W

G

G

P

P

L

M

G

G

Y

M

V

V

V

V

E

E

N

G

K

K

L

S

S

Y

F

W

Y

F

R

R

L

L

P

P

A

A

R

K

P

R

H

H

T

T

T

V

Q

N

T

S

E

E

F

F

D

D

I

I

D

K

K

Q

I

I

S

S

S

S

L

L

P

P

R

Q

V

V

D

D

I

I

V

A

Y

Y

N

G

Y

Y

G

G

N

N

V

V

S

T

R

D

T

T

A

A

Y

Y

D

K

A

A

F

L

V

A

A

Q

A

N

G

G

A

A

K

K

A

A

I

L

I

I

H

H

D

A

G

G

T

T

G

G

N

N

G

G

S

S

V

V

A

S

E

S

Q

R

I

V

V

V

P

P

V

A

L

L

Q

Q

E

E

V

L

R

R

S

K

K

N

G

G

V

V

Q

Q

V

I

I

I

R

R

A

S

S

S

R

H

T

V

G

N

S

Q

-

G

G

G

V

F

V

V

I

L

R

R

N

N

G

A

E
|
E

Q

Q

P

P

D

D

D

D

K

K

F

N

D

D

W

W

V

V

V

V

T

A

D

H

D

D

Q

L

N

N

A

P

Q

Q

K

K

A

A

R

R

I

I

L

L

A

A

E

M

L

V

A

A

L

M

T

T

K

K

T

T

N

Q

D

D

P

S

K

K

E

E

L

L

Q

Q

K

R

I

I

M

F

W

W

K

E

Y

Y

active site: T12 (= T40), T75 (= T120), D76 (= D121), M148 (≠ K193), E269 (= E313)
binding d-glutamic acid: T12 (= T40), A41 (≠ G86), S42 (= S87), E43 (= E88), G74 (= G119), T75 (= T120), D76 (= D121), S100 (= S145)

P10182 Glutaminase-asparaginase; L-ASNase/L-GLNase; L-asparagine/L-glutamine amidohydrolase; PGA; EC 3.5.1.38 from Pseudomonas sp. (strain ATCC 29598 / 7A) (see paper)
63% identity, 94% coverage: 24:356/356 of query aligns to 4:337/337 of P10182

query
sites
P10182

Q

E

A

N

Q

Q

D

Q

K

K

K

L

P

A

N

N

V

V

V

V

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

A

G

G

A

A

S

S

S

A

V

A

N

N

T

S

A

A

A

T

Y

Y

Q

Q

S

A

A

A

V

K

V

V

P

G

V

V

D

D

K

K

I

L

I

I

A

A

A

G

V

V

P

P

E

E

I

L

S

A

K

D

I

L

A

A

N

N

V

V

R

R

G

G

E

E

Q

Q

I

V

F

M

Q

Q

I

I

G

A

S

S

E

E

S

S

F

I

N

T

D

N

E

D

R

D

L

L

I

L

K

K

L

L

G

G

K

K

R

R

V

V

S

A

E

E

L

L

L

A

K

D

Q

S

N

N

D

D

V

V

D

D

G

G

I

I

V

V

I

I

T

T

H

H

G

G

T

T

D

D

T

T

I

L

E

E

E

E

T

T

A

A

Y

Y

F

F

L

L

N

D

L

L

V

T

L

L

K

N

S

T

D

D

K

K

P

P

V

I

V

V

V

V

V

V

G

G

S

S

M

M

R

R

P

P

G

G

T

T

A

A

L

M

G

S

A

A

D

D

G

G

A

M

L

L

N

N

L

L

Y

Y

D

N

A

A

V

V

L

A

V

V

A

A

G

S

S

N

K

K

E

D

A

S

A

R

G

G

K

K

G

G

T

V

L

L

V

V

V

T

M

M

N

N

D

D

E

E

I

I

H

Q

S

S

G

G

R

R

D

D

V

V

T

S

K

K

S

S

N

I

T

N

F

I

K

K

V

T

E

E

T

A

F

F

R

K

S

S

P

A

Y

W

G

G

P

P

L

L

G

G

Y

M

V

V

V

V

E

E

N

G

K

K

L

S

S

Y

F

W

Y

F

R

R

L

L

P

P

A

A

R

K

P

R

H

H

T

T

T

V

Q

N

T

S

E

E

F

F

D

D

I

I

D

K

K

Q

I

I

S

S

S

S

L

L

P

P

R

Q

V

V

D

D

I

I

V

A

Y

Y

N

S

Y

Y

G

G

N

N

V

V

S

T

R

D

T

T

A

A

Y

Y

D

K

A

A

F

L

V

A

A

Q

A

N

G

G

A

A

K

K

A

A

I

L

I

I

H

H

D

A

G

G

T

T

G

G

N

N

G

G

S

S

V

V

A

S

E

S

Q

R

I

L

V

T

P

P

V

A

L

L

Q

Q

E

T

V

L

R

R

S

K

K

T

G

G

V

T

Q

Q

V

I

I

I

R

R

A

S

S

S

R

H

T

V

G

N

S

Q

-

G

G

G

V

F

V

V

I

L

R

R

N

N

G

A

E

E

Q

Q

P

P

D

D

D

D

K

K

F

N

D

D

W

W

V

V

V

V

T

A

D

H

D

D

Q

L

N

N

A

P

Q

E

K

K

A

A

R

R

I

I

L

L

A

V

E

E

L

L

A

A

L

M

T

V

K

K

T

T

N

Q

D

D

P

S

K

K

E

E

L

L

Q

Q

K

R

I

I

M

F

W

W

K

E

Y

Y

T20 (= T40) active site, Acyl-ester intermediate

5k45A Wolinella succinogenes l-asparaginase p121 + l-glutamic acid (see paper)
49% identity, 92% coverage: 29:356/356 of query aligns to 1:328/328 of 5k45A

query
sites
5k45A

K

K

P

P

N

Q

V

V

V

T

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

S

G

G

A

E

S

S

S

S

V

V

N

K

T

S

A

S

A

-

Y
|
Y

Q

S

S

A

A

G

V

A

V

V

P

T

V

V

D

D

K

K

I

L

I

L

A

A

A

A

V

V

P

P

E

A

I

I

S

N

K

D

I

L

A

A

N

T

V

I

R

K

G

G

E

E

Q

Q

I

I

F

S

Q

S

I

I

G
|
G

S
|
S

E
x
Q

S

E

F

M

N

T

D

G

E

K

R

V

L

W

I

L

K

K

L

L

G

A

K

K

R

R

V

V

S

N

E

E

L

L

L

L

K

A

Q

Q

N

K

D

E

V

T

D

E

G

A

I

V

V

I

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

M

E

E

E

E

T

T

A

A

Y

F

F

F

L

L

N

N

L

L

V

T

L

V

K

K

S

S

D

Q

K

K

P

P

V

V

V

V

V

L

V

V

G

G

S
x
A

M

M

R

R

P

P

G

G

T

S

A

S

L

M

G

S

A

A

D

D

G

G

A

P

L

M

N

N

L

L

Y

Y

D

N

A

A

V

V

L

N

V

V

A

A

G

I

S

N

K

K

E

A

A

S

A

T

G

N

K

K

G

G

T

V

L

V

V

I

V

V

M

M

N

N

D

D

E

E

I

I

H

H

S

A

G

A

R

R

D

E

V

A

T

T

K
|
K

S

L

N

N

T

T

F

T

K

A

V

V

E

N

T

A

F

F

R

A

S

S

P

P

-

N

Y

T

G

G

P

K

L

I

G

G

Y

T

V

V

V

Y

E

Y

N

G

K

K

L

V

S

E

F

Y

Y

F

R

T

L

Q

P

S

A

V

R

R

P

P

H

H

T

T

T

L

Q

A

T

S

E

E

F

F

D

D

I

I

D

S

K

K

I

I

S

E

S

E

L

L

P

P

R

R

V

V

D

D

I

I

V

L

Y

Y

N

A

Y

H

G

P

N

D

V

D

S

T

R

D

T

V

A

L

Y

V

D

N

A

A

F

A

V

L

A

Q

A

A

G

G

A

A

K

K

A

G

I

I

I

I

H

H

D

A

G

G

T

M

G

G

N

N

G

G

S

N

V

P

A

F

E

P

Q

L

I

T

V

Q

P

N

V

A

L

L

Q

E

E

K

V

A

R

A

S

K

K

S

G

G

V

V

Q

V

V

V

I

A

R

R

A

S

S

S

R

R

T

V

G

G

S

S

G

G

V

S

V

T

I

T

R

Q

N

E

G

A

E
|
E

Q

V

P

D

D

D

D

K

K

K

F

L

D

G

W

F

V

V

V

A

T

T

D

E

D

S

Q

L

N

N

A

P

Q

Q

K

K

A

A

R

R

I

V

L

L

A

L

E

M

L

L

A

A

L

L

T

T

K

K

T

T

N

S

D

D

P

R

K

E

E

A

L

I

Q

Q

K

K

I

I

M

F

W

S

K

T

Y

Y

active site: T12 (= T40), Y25 (= Y54), T91 (= T120), D92 (= D121), K164 (= K193), E285 (= E313)
binding glutamic acid: G57 (= G86), S58 (= S87), Q59 (≠ E88), G90 (= G119), T91 (= T120), D92 (= D121), A116 (≠ S145)

5k3oA Wolinella succinogenes l-asparaginase p121 and l-aspartic acid (see paper)
49% identity, 92% coverage: 29:356/356 of query aligns to 1:328/328 of 5k3oA

query
sites
5k3oA

K

K

P

P

N

Q

V

V

V

T

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

S

G

G

A

E

S

S

S

S

V

V

N

K

T

S

A

S

A

-

Y
|
Y

Q

S

S

A

A

G

V

A

V

V

P

T

V

V

D

D

K

K

I

L

I

L

A

A

A

A

V

V

P

P

E

A

I

I

S

N

K

D

I

L

A

A

N

T

V

I

R

K

G

G

E

E

Q

Q

I

I

F

S

Q

S

I

I

G
|
G

S
|
S

E
x
Q

S

E

F

M

N

T

D

G

E

K

R

V

L

W

I

L

K

K

L

L

G

A

K

K

R

R

V

V

S

N

E

E

L

L

L

L

K

A

Q

Q

N

K

D

E

V

T

D

E

G

A

I

V

V

I

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

M

E

E

E

E

T

T

A

A

Y

F

F

F

L

L

N

N

L

L

V

T

L

V

K

K

S

S

D

Q

K

K

P

P

V

V

V

V

V

L

V

V

G

G

S

A

M

M

R

R

P

P

G

G

T

S

A

S

L

M

G

S

A

A

D

D

G

G

A

P

L

M

N

N

L

L

Y

Y

D

N

A

A

V

V

L

N

V

V

A

A

G

I

S

N

K

K

E

A

A

S

A

T

G

N

K

K

G

G

T

V

L

V

V

I

V

V

M

M

N

N

D

D

E

E

I

I

H

H

S

A

G

A

R

R

D

E

V

A

T

T

K
|
K

S

L

N

N

T

T

F

T

K

A

V

V

E

N

T

A

F

F

R

A

S

S

P

P

-

N

Y

T

G

G

P

K

L

I

G

G

Y

T

V

V

V

Y

E

Y

N

G

K

K

L

V

S

E

F

Y

Y

F

R

T

L

Q

P

S

A

V

R

R

P

P

H

H

T

T

T

L

Q

A

T

S

E

E

F

F

D

D

I

I

D

S

K

K

I

I

S

E

S

E

L

L

P

P

R

R

V

V

D

D

I

I

V

L

Y

Y

N

A

Y

H

G

P

N

D

V

D

S

T

R

D

T

V

A

L

Y

V

D

N

A

A

F

A

V

L

A

Q

A

A

G

G

A

A

K

K

A

G

I

I

I

I

H

H

D

A

G

G

T

M

G

G

N

N

G

G

S

N

V

P

A

F

E

P

Q

L

I

T

V

Q

P

N

V

A

L

L

Q

E

E

K

V

A

R

A

S

K

K

S

G

G

V

V

Q

V

V

V

I

A

R

R

A

S

S

S

R

R

T

V

G

G

S

S

G

G

V

S

V

T

I

T

R

Q

N

E

G

A

E
|
E

Q

V

P

D

D

D

D

K

K

K

F

L

D

G

W

F

V

V

V

A

T

T

D

E

D

S

Q

L

N

N

A

P

Q

Q

K

K

A

A

R

R

I

V

L

L

A

L

E

M

L

L

A

A

L

L

T

T

K

K

T

T

N

S

D

D

P

R

K

E

E

A

L

I

Q

Q

K

K

I

I

M

F

W

S

K

T

Y

Y

active site: T12 (= T40), Y25 (= Y54), T91 (= T120), D92 (= D121), K164 (= K193), E285 (= E313)
binding aspartic acid: T12 (= T40), G57 (= G86), S58 (= S87), Q59 (≠ E88), G90 (= G119), T91 (= T120), D92 (= D121)

P06608 L-asparaginase; L-ASNase; L-asparagine amidohydrolase; EC 3.5.1.1 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see 2 papers)
46% identity, 94% coverage: 22:356/356 of query aligns to 18:348/348 of P06608

query
sites
P06608

S

T

A

A

Q

S

A

A

Q

A

D

D

K

K

K

L

P

P

N

N

V

I

V

V

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

S

G

A

A

A

S

T

S

G

V

T

N

Q

T

T

A

T

A

G

Y

Y

Q

K

S

A

A

G

V

A

V

L

P

G

V

V

D

D

K

T

I

L

I

I

A

N

A

A

V

V

P

P

E

E

I

V

S

K

K

K

I

L

A

A

N

N

V

V

R

K

G

G

E

E

Q

Q

I

F

F

S

Q

N

I

M

G

A

S

S

E

E

S

N

F

M

N

T

D

G

E

D

R

V

L

V

I

L

K

K

L

L

G

S

K

Q

R

R

V

V

S

N

E

E

L

L

L

L

K

A

Q

R

N

D

D

D

V

V

D

D

G

G

I

V

V

V

I

I

T

T

H

H

G

G

T

T

D

D

T

T

I

V

E

E

E

E

T

S

A

A

Y

Y

F

F

L

L

N

H

L

L

V

T

L

V

K

K

S

S

D

D

K

K

P

P

V

V

V

V

V

F

V

V

G

A

S

A

M

M

R

R

P

P

G

A

T

T

A

A

L

I

G

S

A

A

D

D

G

G

A

P

L

M

N

N

L

L

Y

L

D

E

A

A

V

V

L

R

V

V

A

A

G

G

S

D

K

K

E

Q

A

S

A

R

G

G

K

R

G

G

T

V

L

M

V

V

V

V

M

L

N

N

D

D

E

R

I

I

H

G

S

S

G

A

R

R

D

Y

V

I

T

T

K

K

S

T

N

N

T

A

F

S

K

T

V

L

E

D

T

T

F

F

R

K

S

A

-

N

P

E

Y

E

G

G

P

Y

L

L

G

G

Y

V

V

I

V

I

E

G

N

N

K

R

L

I

S

Y

F

Y

Y

Q

R

N

L

R

P

I

A

D

R

K

P

L

H

H

T

T

T

T

Q

R

T

S

E

V

F

F

D

D

I

V

D

R

K

G

I

L

S

T

S

S

L

L

P

P

R

K

V

V

D

D

I

I

V

L

Y

Y

N

G

Y

Y

G

Q

N

D

V

D

S

P

R

E

T

Y

A

L

Y

Y

D

D

A

A

F

A

V

I

A

Q

A

H

G

G

A

V

K

K

A

G

I

I

I

V

H

Y

D

A

G

G

T

M

G

G

N

A

G

G

S

S

V

V

A

S

E

V

Q

R

I

G

V

I

P

A

V

G

L

M

Q

R

E

K

V

A

R

M

S

E

K

K

G

G

V

V

Q

V

V

V

I

I

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

I

-

R

-

N

-

G

-

E

-

Q

P

P

P

D

D

D

E

K

E

F

L

D

P

W

G

V

L

V

V

T

S

D

D

D

S

Q

L

N

N

A

P

Q

A

K

H

A

A

R

R

I

I

L

L

A

L

E

M

L

L

A

A

L

L

T

T

K

R

T

T

N

S

D

D

P

P

K

K

E

V

L

I

Q

Q

K

E

I

Y

M

F

W

H

K

T

Y

Y

T36 (= T40) active site, O-isoaspartyl threonine intermediate

1hg0A X-ray structure of the complex between erwinia chrysanthemi l- asparaginase and succinic acid (see paper)
46% identity, 93% coverage: 27:356/356 of query aligns to 2:327/327 of 1hg0A

query
sites
1hg0A

D

D

K

K

K

L

P

P

N

N

V

I

V

V

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

S

G

A

A

A

S

T

S

G

V

T

N

Q

T

T

A

T

A

G

Y
|
Y

Q

K

S

A

A

G

V

A

V

L

P

G

V

V

D

D

K

T

I

L

I

I

A

N

A

A

V

V

P

P

E

E

I

V

S

K

K

K

I

L

A

A

N

N

V

V

R

K

G

G

E

E

Q

Q

I

F

F

S

Q

N

I

M

G
x
A

S
|
S

E

E

S

N

F

M

N

T

D

G

E

D

R

V

L

V

I

L

K

K

L

L

G

S

K

Q

R

R

V

V

S

N

E

E

L

L

L

L

K

A

Q

R

N

D

D

D

V

V

D

D

G

G

I

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

V

E

E

E

E

T

S

A

A

Y

Y

F

F

L

L

N

H

L

L

V

T

L

V

K

K

S

S

D

D

K

K

P

P

V

V

V

V

V

F

V

V

G

A

S

A

M

M

R

R

P

P

G

A

T

T

A

A

L

I

G

S

A

A

D

D

G

G

A

P

L

M

N

N

L

L

Y

L

D

E

A

A

V

V

L

R

V

V

A

A

G

G

S

D

K

K

E

Q

A

S

A

R

G

G

K

R

G

G

T

V

L

M

V

V

V

V

M

I

N

N

D

D

E

R

I

I

H

G

S

S

G

A

R

R

D

Y

V

I

T

T

K
|
K

S

T

N

N

T

A

F

S

K

T

V

L

E

D

T

T

F

F

R

R

S

A

-

N

P

E

Y

E

G

G

P

Y

L

L

G

G

Y

V

V

I

V

I

E

G

N

N

K

R

L

I

S

Y

F

Y

Y

Q

R

N

L

R

P

I

A

D

R

K

P

L

H

H

T

T

T

T

Q

R

T

S

E

V

F

F

D

D

I

V

D

R

K

G

I

L

S

T

S

S

L

L

P

P

R

K

V

V

D

D

I

I

V

L

Y

Y

N

G

Y

Y

G

Q

N

D

V

D

S

P

R

E

T

Y

A

L

Y

Y

D

D

A

A

F

A

V

I

A

Q

A

H

G

G

A

V

K

K

A

G

I

I

I

V

H

Y

D

A

G

G

T

M

G

G

N

A

G

G

S

S

V

V

A

S

E

V

Q

R

I

G

V

I

P

A

V

G

L

M

Q

R

E

K

V

A

R

L

S

E

K

K

G

G

V

V

Q

V

V

V

I

M

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

I

-

R

-

N

-

G

-

E

-

Q

P

P

P

D

D

D

E

K

E

F

L

D

P

W

G

V

L

V

V

T

S

D

D

D

S

Q

L

N

N

A

P

Q

A

K

H

A

A

R

R

I

I

L

L

A

L

E

M

L

L

A

A

L

L

T

T

K

R

T

T

N

S

D

D

P

P

K

K

E

V

L

I

Q

Q

K

E

I

Y

M

F

W

H

K

T

Y

Y

active site: T15 (= T40), Y29 (= Y54), T95 (= T120), D96 (= D121), K168 (= K193)
binding succinic acid: T15 (= T40), A61 (≠ G86), S62 (= S87), G94 (= G119), T95 (= T120), D96 (= D121)

1jsrA Crystal structure of erwinia chrysanthemi l-asparaginase complexed with 6-hydroxy-l-norleucine (see paper)
46% identity, 92% coverage: 30:356/356 of query aligns to 2:324/324 of 1jsrA

query
sites
1jsrA

P

P

N

N

V

I

V

V

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

S

G

A

A

A

S

T

S

G

V

T

N

Q

T

T

A

T

A

G

Y
|
Y

Q

K

S

A

A

G

V

A

V

L

P

G

V

V

D

D

K

T

I

L

I

I

A

N

A

A

V

V

P

P

E

E

I

V

S

K

K

K

I

L

A

A

N

N

V

V

R

K

G

G

E

E

Q

Q

I

F

F

S

Q

N

I

M

G
x
A

S
|
S

E
|
E

S

N

F

M

N

T

D

G

E

D

R

V

L

V

I

L

K

K

L

L

G

S

K

Q

R

R

V

V

S

N

E

E

L

L

L

L

K

A

Q

R

N

D

D

D

V

V

D

D

G

G

I

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

V

E

E

E

E

T

S

A

A

Y

Y

F

F

L

L

N

H

L

L

V

T

L

V

K

K

S

S

D

D

K

K

P

P

V

V

V

V

V

F

V

V

G

A

S

A

M

M

R

R

P

P

G

A

T

T

A

A

L

I

G

S

A

A

D

D

G

G

A

P

L

M

N

N

L

L

Y

L

D

E

A

A

V

V

L

R

V

V

A

A

G

G

S

D

K

K

E

Q

A

S

A

R

G

G

K

R

G

G

T

V

L

M

V

V

V

V

M

I

N

N

D

D

E

R

I

I

H

G

S

S

G

A

R

R

D

Y

V

I

T

T

K
|
K

S

T

N

N

T

A

F

S

K

T

V

L

E

D

T

T

F

F

R

R

S

A

-

N

P

E

Y

E

G

G

P

Y

L

L

G

G

Y

V

V

I

V

I

E

G

N

N

K

R

L

I

S

Y

F

Y

Y

Q

R

N

L

R

P

I

A

D

R

K

P

L

H

H

T

T

T

T

Q

R

T

S

E

V

F

F

D

D

I

V

D

R

K

G

I

L

S

T

S

S

L

L

P

P

R

K

V

V

D

D

I

I

V

L

Y

Y

N

G

Y

Y

G

Q

N

D

V

D

S

P

R

E

T

Y

A

L

Y

Y

D

D

A

A

F

A

V

I

A

Q

A

H

G

G

A

V

K

K

A

G

I

I

I

V

H

Y

D

A

G

G

T

M

G

G

N

A

G

G

S

S

V

V

A

S

E

V

Q

R

I

G

V

I

P

A

V

G

L

M

Q

R

E

K

V

A

R

L

S

E

K

K

G

G

V

V

Q

V

V

V

I

M

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

I

-

R

-

N

-

G

-

E

-

Q

P

P

P

D

D

D

E

K

E

F

L

D

P

W

G

V

L

V

V

T

S

D

D

D

S

Q

L

N

N

A

P

Q

A

K

H

A

A

R

R

I

I

L

L

A

L

E

M

L

L

A

A

L

L

T

T

K

R

T

T

N

S

D

D

P

P

K

K

E

V

L

I

Q

Q

K

E

I

Y

M

F

W

H

K

T

Y

Y

active site: T12 (= T40), Y26 (= Y54), T92 (= T120), D93 (= D121), K165 (= K193)
binding 6-hydroxy-l-norleucine: T12 (= T40), Y26 (= Y54), A58 (≠ G86), S59 (= S87), E60 (= E88), G91 (= G119), T92 (= T120), D93 (= D121)

1jslA Crystal structure of erwinia chrysanthemi l-asparaginase complexed with 6-hydroxy-d-norleucine (see paper)
46% identity, 92% coverage: 30:356/356 of query aligns to 2:324/324 of 1jslA

query
sites
1jslA

P

P

N

N

V

I

V

V

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

S

G

A

A

A

S

T

S

G

V

T

N

Q

T

T

A

T

A

G

Y
|
Y

Q

K

S

A

A

G

V

A

V

L

P

G

V

V

D

D

K

T

I

L

I

I

A

N

A

A

V

V

P

P

E

E

I

V

S

K

K

K

I

L

A

A

N

N

V

V

R

K

G

G

E

E

Q

Q

I

F

F

S

Q

N

I

M

G
x
A

S
|
S

E
|
E

S

N

F

M

N

T

D

G

E

D

R

V

L

V

I

L

K

K

L

L

G

S

K

Q

R

R

V

V

S

N

E

E

L

L

L

L

K

A

Q

R

N

D

D

D

V

V

D

D

G

G

I

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

V

E

E

E

E

T

S

A

A

Y

Y

F

F

L

L

N

H

L

L

V

T

L

V

K

K

S

S

D

D

K

K

P

P

V

V

V

V

V

F

V

V

G

A

S
x
A

M

M

R

R

P

P

G

A

T

T

A

A

L

I

G

S

A

A

D

D

G

G

A

P

L

M

N

N

L

L

Y

L

D

E

A

A

V

V

L

R

V

V

A

A

G

G

S

D

K

K

E

Q

A

S

A

R

G

G

K

R

G

G

T

V

L

M

V

V

V

V

M

I

N

N

D

D

E

R

I

I

H

G

S

S

G

A

R

R

D

Y

V

I

T

T

K
|
K

S

T

N

N

T

A

F

S

K

T

V

L

E

D

T

T

F

F

R

R

S

A

-

N

P

E

Y

E

G

G

P

Y

L

L

G

G

Y

V

V

I

V

I

E

G

N

N

K

R

L

I

S

Y

F

Y

Y

Q

R

N

L

R

P

I

A

D

R

K

P

L

H

H

T

T

T

T

Q

R

T

S

E

V

F

F

D

D

I

V

D

R

K

G

I

L

S

T

S

S

L

L

P

P

R

K

V

V

D

D

I

I

V

L

Y

Y

N

G

Y

Y

G

Q

N

D

V

D

S

P

R

E

T

Y

A

L

Y

Y

D

D

A

A

F

A

V

I

A

Q

A

H

G

G

A

V

K

K

A

G

I

I

I

V

H

Y

D

A

G

G

T

M

G

G

N

A

G

G

S

S

V

V

A

S

E

V

Q

R

I

G

V

I

P

A

V

G

L

M

Q

R

E

K

V

A

R

L

S

E

K

K

G

G

V

V

Q

V

V

V

I

M

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

I

-

R

-

N

-

G

-

E

-

Q

P

P

P

D

D

D

E

K

E

F

L

D

P

W

G

V

L

V

V

T

S

D

D

D

S

Q

L

N

N

A

P

Q

A

K

H

A

A

R

R

I

I

L

L

A

L

E

M

L

L

A

A

L

L

T

T

K

R

T

T

N

S

D

D

P

P

K

K

E

V

L

I

Q

Q

K

E

I

Y

M

F

W

H

K

T

Y

Y

active site: T12 (= T40), Y26 (= Y54), T92 (= T120), D93 (= D121), K165 (= K193)
binding 6-hydroxy-d-norleucine: T12 (= T40), Y26 (= Y54), A58 (≠ G86), S59 (= S87), E60 (= E88), G91 (= G119), T92 (= T120), D93 (= D121), A117 (≠ S145)

7u6mC Albumin binding domain fused to a mutant of the erwinia asparaginase (see paper)
46% identity, 93% coverage: 27:356/356 of query aligns to 17:342/342 of 7u6mC

query
sites
7u6mC

D

D

K

K

K

L

P

P

N

N

V

I

V

V

V

I

I

L

G

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

A

S

G

A

A

A

S

T

S

G

V

T

N

Q

T

T

A

T

A

G

Y

Y

Q

K

S

I

A

G

V

A

V

L

P

G

V

V

D

D

K

T

I

L

I

I

A

N

A

A

V

V

P

P

E

E

I

V

S

K

K

K

I

L

A

A

N

N

V

V

R

K

G

G

E

E

Q

Q

I

F

F

S

Q

N

I

M

G
x
A

S
|
S

E
x
Q

S

N

F

M

N

T

D

G

E

D

R

V

L

V

I

L

K

K

L

L

G

S

K

Q

R

R

V

V

S

N

E

E

L

L

L

L

K

A

Q

R

N

D

D

D

V

V

D

D

G

G

I

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

I

V

E

E

E

E

T

S

A

A

Y

Y

F

F

L

L

N

H

L

L

V

T

L

V

K

K

S

S

D

D

K

K

P

P

V

V

V

V

V

F

V

V

G

A

S

A

M

M

R

R

P

P

G

A

T

T

A

A

L

I

G

S

A

A

D

D

G

G

A

P

L

M

N

N

L

L

Y

L

D

E

A

A

V

V

L

R

V

V

A

A

G

G

S

D

K

K

E

Q

A

S

A

R

G

G

K

R

G

G

T

V

L

M

V

V

V

V

M

L

N

N

D

D

E

R

I

I

H

G

S

S

G

A

R

R

D

Y

V

I

T

T

K

K

S

T

N

N

T

A

F

S

K

T

V

L

E

D

T

T

F

F

R

K

S

A

-

N

P

E

Y

E

G

G

P

Y

L

L

G

G

Y

V

V

I

V

I

E

G

N

N

K

R

L

I

S

Y

F

Y

Y

Q

R

N

L

R

P

I

A

D

R

K

P

L

H

H

T

T

T

T

Q

R

T

S

E

V

F

F

D

D

I

V

D

R

K

G

I

L

S

T

S

S

L

L

P

P

R

K

V

V