SitesBLAST
Comparing HSERO_RS07550 FitnessBrowser__HerbieS:HSERO_RS07550 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
44% identity, 98% coverage: 1:312/319 of query aligns to 1:315/334 of 5aovA
- active site: L100 (= L99), R241 (= R237), D265 (= D261), E270 (= E266), H288 (= H285)
- binding glyoxylic acid: M52 (≠ A52), L53 (vs. gap), L53 (vs. gap), Y74 (≠ I73), A75 (≠ S74), V76 (= V75), G77 (= G76), R241 (= R237), H288 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V75), T104 (= T103), F158 (≠ M153), G159 (= G154), R160 (= R155), I161 (= I156), S180 (≠ N176), R181 (= R177), A211 (≠ M207), V212 (≠ L208), P213 (= P209), T218 (= T214), I239 (≠ A235), A240 (≠ S236), R241 (= R237), H288 (= H285), G290 (= G287)
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
46% identity, 85% coverage: 43:314/319 of query aligns to 49:322/328 of Q9UBQ7
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
46% identity, 85% coverage: 43:314/319 of query aligns to 45:318/324 of 2gcgA
- active site: L103 (= L99), R241 (= R237), D265 (= D261), E270 (= E266), H289 (= H285)
- binding (2r)-2,3-dihydroxypropanoic acid: L55 (vs. gap), S78 (= S74), V79 (= V75), G80 (= G76), R241 (= R237), H289 (= H285)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (= V75), T107 (= T103), G156 (= G152), G158 (= G154), I160 (= I156), G180 (≠ N176), R181 (= R177), R184 (≠ D180), C212 (≠ L208), S213 (≠ P209), T218 (= T214), I239 (≠ A235), R241 (= R237), D265 (= D261), H289 (= H285), G291 (= G287)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
43% identity, 97% coverage: 2:312/319 of query aligns to 1:314/332 of 6biiA
- active site: L99 (= L99), R240 (= R237), D264 (= D261), E269 (= E266), H287 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V75), T103 (= T103), G156 (= G152), F157 (≠ M153), G158 (= G154), R159 (= R155), I160 (= I156), A179 (≠ N176), R180 (= R177), S181 (≠ R178), K183 (≠ D180), V211 (≠ L208), P212 (= P209), E216 (≠ Q213), T217 (= T214), V238 (≠ A235), A239 (≠ S236), R240 (= R237), D264 (= D261), H287 (= H285), G289 (= G287)
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
44% identity, 98% coverage: 1:312/319 of query aligns to 1:315/333 of 2dbqA
- active site: L100 (= L99), R241 (= R237), D265 (= D261), E270 (= E266), H288 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V75), T104 (= T103), L158 (≠ M153), G159 (= G154), R160 (= R155), I161 (= I156), S180 (≠ N176), R181 (= R177), T182 (≠ R178), A211 (≠ M207), V212 (≠ L208), P213 (= P209), T218 (= T214), I239 (≠ A235), A240 (≠ S236), R241 (= R237), D265 (= D261), H288 (= H285), G290 (= G287)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
44% identity, 98% coverage: 1:312/319 of query aligns to 1:315/334 of O58320
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
39% identity, 97% coverage: 5:314/319 of query aligns to 6:308/313 of Q65CJ7
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
39% identity, 97% coverage: 5:314/319 of query aligns to 4:306/311 of 3bazA
- active site: L98 (= L99), R230 (= R237), A251 (= A258), D254 (= D261), E259 (= E266), H277 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (= V75), G149 (= G152), L150 (≠ M153), G151 (= G154), R152 (= R155), I153 (= I156), S172 (≠ N176), R173 (= R177), S174 (≠ R178), C201 (≠ L208), P202 (= P209), T207 (= T214), I228 (≠ A235), G229 (≠ S236), R230 (= R237), D254 (= D261), H277 (= H285), G279 (= G287)
5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
38% identity, 96% coverage: 2:307/319 of query aligns to 1:297/317 of 5v7gA
- active site: L94 (= L99), R228 (= R237), D252 (= D261), E257 (= E266), H275 (= H285)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (= V75), V98 (≠ T103), F146 (≠ I151), L148 (≠ M153), G149 (= G154), R150 (= R155), I151 (= I156), T170 (≠ N176), R171 (= R177), V199 (≠ L208), P200 (= P209), S204 (≠ Q213), T205 (= T214), V226 (≠ A235), G227 (≠ S236), R228 (= R237), H275 (= H285), A277 (≠ G287)
- binding oxalate ion: G69 (≠ S74), V70 (= V75), G71 (= G76), R228 (= R237), H275 (= H285)
5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
38% identity, 96% coverage: 2:307/319 of query aligns to 3:299/319 of 5v6qB
- active site: L96 (= L99), R230 (= R237), D254 (= D261), E259 (= E266), H277 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (= V75), V100 (≠ T103), F148 (≠ I151), L150 (≠ M153), G151 (= G154), R152 (= R155), I153 (= I156), T172 (≠ N176), R173 (= R177), V201 (≠ L208), P202 (= P209), S206 (≠ Q213), T207 (= T214), V228 (≠ A235), G229 (≠ S236), R230 (= R237), H277 (= H285), A279 (≠ G287)
5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
38% identity, 96% coverage: 2:307/319 of query aligns to 2:298/319 of 5v7nA
- active site: L95 (= L99), R229 (= R237), D253 (= D261), E258 (= E266), H276 (= H285)
- binding 2-keto-D-gluconic acid: G70 (≠ S74), V71 (= V75), G72 (= G76), R229 (= R237), H276 (= H285), S279 (= S288), R285 (= R294)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (= V75), V99 (≠ T103), L149 (≠ M153), G150 (= G154), R151 (= R155), I152 (= I156), T171 (≠ N176), R172 (= R177), V200 (≠ L208), P201 (= P209), S205 (≠ Q213), T206 (= T214), V227 (≠ A235), G228 (≠ S236), R229 (= R237), H276 (= H285), A278 (≠ G287)
5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
38% identity, 96% coverage: 2:307/319 of query aligns to 1:297/318 of 5j23A
- active site: L94 (= L99), R228 (= R237), D252 (= D261), E257 (= E266), H275 (= H285)
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (= V75), L148 (≠ M153), G149 (= G154), R150 (= R155), I151 (= I156), T170 (≠ N176), R171 (= R177), P200 (= P209), S204 (≠ Q213), T205 (= T214), R228 (= R237)
4e5pA Thermostable phosphite dehydrogenase a176r variant in complex with nad (see paper)
32% identity, 98% coverage: 1:312/319 of query aligns to 1:319/332 of 4e5pA
- active site: L100 (= L99), R237 (= R237), D261 (= D261), E266 (= E266), H292 (= H285)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V75), L100 (= L99), T104 (= T103), G154 (= G154), A155 (≠ R155), I156 (= I156), A175 (≠ N176), R176 (= R177), L208 (= L208), P209 (= P209), T214 (= T214), P235 (≠ A235), C236 (≠ S236), R237 (= R237), H292 (= H285)
4e5mA Thermostable phosphite dehydrogenase e175a/a176r in complex with NADP (see paper)
32% identity, 98% coverage: 1:312/319 of query aligns to 1:319/329 of 4e5mA
- active site: L100 (= L99), R237 (= R237), D261 (= D261), E266 (= E266), H292 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K76 (≠ V75), L100 (= L99), T104 (= T103), G154 (= G154), A155 (≠ R155), I156 (= I156), R176 (= R177), L208 (= L208), P209 (= P209), T214 (= T214), P235 (≠ A235), C236 (≠ S236), R237 (= R237), H292 (= H285), G294 (= G287)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
35% identity, 91% coverage: 4:294/319 of query aligns to 2:287/304 of 1wwkA
- active site: S96 (≠ L99), R230 (= R237), D254 (= D261), E259 (= E266), H278 (= H285)
- binding nicotinamide-adenine-dinucleotide: V100 (≠ T103), G146 (= G152), F147 (≠ M153), G148 (= G154), R149 (= R155), I150 (= I156), Y168 (≠ H175), D169 (≠ N176), P170 (≠ R177), V201 (≠ L208), P202 (= P209), T207 (= T214), T228 (≠ A235), S229 (= S236), D254 (= D261), H278 (= H285), G280 (= G287)
4e5kA Thermostable phosphite dehydrogenase in complex with NAD and sulfite (see paper)
32% identity, 98% coverage: 1:312/319 of query aligns to 1:319/329 of 4e5kA
- active site: L100 (= L99), R237 (= R237), D261 (= D261), E266 (= E266), H292 (= H285)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V75), G77 (= G76), L100 (= L99), T104 (= T103), G152 (= G152), G154 (= G154), A155 (≠ R155), I156 (= I156), H174 (= H175), E175 (≠ N176), A176 (≠ R177), A207 (≠ M207), L208 (= L208), P209 (= P209), P235 (≠ A235), C236 (≠ S236), R237 (= R237), D261 (= D261), H292 (= H285), G294 (= G287)
- binding sulfite ion: M53 (≠ L54), L75 (≠ S74), K76 (≠ V75), G77 (= G76), L100 (= L99), R237 (= R237), H292 (= H285)
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
32% identity, 87% coverage: 35:311/319 of query aligns to 33:305/305 of 6plfA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
33% identity, 83% coverage: 35:299/319 of query aligns to 32:292/303 of 6plgA
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
33% identity, 83% coverage: 35:299/319 of query aligns to 31:291/297 of 6rj3A
7dkmA Phgdh covalently linked to oridonin (see paper)
33% identity, 83% coverage: 35:299/319 of query aligns to 33:293/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V75), A102 (≠ T103), G148 (= G152), R151 (= R155), I152 (= I156), Y170 (≠ H175), D171 (≠ N176), P172 (≠ R177), I173 (≠ R178), H202 (≠ M207), T203 (≠ L208), P204 (= P209), T209 (= T214), C230 (≠ A235), A231 (≠ S236), R232 (= R237), H279 (= H285), G281 (= G287)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: E293 (= E299)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18
Query Sequence
>HSERO_RS07550 FitnessBrowser__HerbieS:HSERO_RS07550
MKPHVVVYKKLPEHLVQRLQAEFDLTLFDRITDDNRADFIAALATAHGMIGASLPITNAM
LEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARRVVE
LAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPD
PEAERELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRI
VDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETRLAMAEL
AVTNLIAGLRGEPVAHLAV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory