SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS08035 FitnessBrowser__HerbieS:HSERO_RS08035 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

8gxfB Pseudomonas flexibilis gcn5 family acetyltransferase (see paper)
30% identity, 96% coverage: 6:193/195 of query aligns to 5:186/187 of 8gxfB

query
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8gxfB

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binding coenzyme a: T93 (= T100), H100 (≠ Q107), G101 (≠ R108), K109 (= K116), T129 (= T136), R135 (≠ Q142)

8gxkB Pseudomonas jinjuensis n-acetyltransferase (see paper)
33% identity, 95% coverage: 6:190/195 of query aligns to 5:183/188 of 8gxkB

query
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8gxkB

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binding coenzyme a: M40 (≠ T42), T93 (= T100), W94 (= W101), H100 (≠ Q107), G101 (≠ R108), T129 (= T136), N133 (= N140), L134 (≠ Q141), R135 (≠ Q142), S136 (= S143), A139 (= A146), K142 (≠ R149)

1yreB Hypothetical protein pa3270 from pseudomonas aeruginosa in complex with coa
32% identity, 95% coverage: 6:190/195 of query aligns to 5:183/187 of 1yreB

query
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1yreB

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binding coenzyme a: M40 (≠ T42), T93 (= T100), W94 (= W101), H100 (≠ Q107), G101 (≠ R108), N105 (= N112), R135 (≠ Q142), G138 (≠ A145)

6c37A Mycobacterium smegmatis rimj in complex with coa-disulfide
29% identity, 83% coverage: 34:195/195 of query aligns to 51:205/209 of 6c37A

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6c37A

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binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: G110 (≠ S99), Y111 (≠ T100), W112 (= W101), V113 (≠ Y102), T118 (≠ Q107), G119 (≠ R108), G121 (= G110), A123 (≠ N112), T124 (= T113), T146 (≠ R135), N151 (= N140), T152 (≠ Q141), P153 (≠ Q142), S154 (= S143), V157 (≠ A146), H160 (≠ R149), R205 (= R195)

Sites not aligning to the query:

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: 42

6c32A Mycobacterium smegmatis rimj with accoa
29% identity, 83% coverage: 34:195/195 of query aligns to 51:205/209 of 6c32A

query
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6c32A

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binding acetyl coenzyme *a: G110 (≠ S99), Y111 (≠ T100), W112 (= W101), V113 (≠ Y102), T118 (≠ Q107), G119 (≠ R108), G121 (= G110), A123 (≠ N112), T124 (= T113), T146 (≠ R135), N151 (= N140), T152 (≠ Q141), P153 (≠ Q142), S154 (= S143), V157 (≠ A146), H160 (≠ R149), R205 (= R195)

Query Sequence

>HSERO_RS08035 FitnessBrowser__HerbieS:HSERO_RS08035
MQFLSPITLKGQFATIEPLHPEHHDALIAAVSDGKLWKLWYTAVPKPENMRAEIERRLHL
QQQGSMLPFVIRRNDTGALCGMTTYMNADSVHRRVEIGSTWYAASAQRTGINTECKLMML
THAFEDMHCIAVEFRTHWMNQQSRAAIARLGAKQDGVLRNHMRMPDGSYRDTVVFSIIES
EWPTVRRHLQFKLGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory