SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS08270 HSERO_RS08270 amino acid ABC transporter substrate-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1uskA L-leucine-binding protein with leucine bound (see paper)
53% identity, 93% coverage: 27:372/373 of query aligns to 1:345/345 of 1uskA

query
sites
1uskA

E

D

T

D

V

I

K

K

I

V

A

A

I

V

A

V

G

G

P

A

M

M

T

S

G

G

A

P

V

I

A

A

Q

Q

Y
x
W

G

G

D

D

M

M

V

E

K

F

A

N

G

G

A

A

L

R

T

Q

A

A

I

I

E

K

Q

D

I

I

N

N

A

A

A

K

G

G

G

G

A

I

N

K

G

G

N

D

K

K

F

L

E

V

A

G

V

V

M

E

M

Y

D

D

D

D

A

A

C

C

E

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

I

V

V

S

N

Q

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

V
x
L

C
|
C

S
|
S

G

S

S

S

T

T

I

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

N

D

E

E

G

G

V

I

V

L

M

M

I

I

T

S

P

P

S
x
G

A
|
A

T
|
T

A

N

P

P

Q

E

L

L

T

T

E

Q

A

-

K

R

P

G

H

Y

K

Q

F

H

I

I

F

M

R

R

T

T

I

A

G

G

R

L

D

D

D

S

Q

S

Q

Q

G

G

P

P

A

T

A

A

A

A

R

K

Y

Y

V

I

I

L

E

E

K

T

L

V

K

K

P

P

K

Q

K

R

V

I

A

A

V

I

L

I

H

H

D

D

K

K

Q

Q

S

Q

Y
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Y

G

G

Q

E

G

G

V

L

A

A

S

R

S

S

V

V

K

Q

A

D

A

G

L

L

D

K

A

A

A

A

K

N

V

A

P

N

V

V

V

V

V

F

F

F

E

D

G

G

I

I

N

T

A

A

G

G

D

E

S

K

D

D

Y

F

S

S

A

A

I

L

V

I

T

A

K

R

L

L

K

K

S

K

Q

E

G

N

V

I

D

D

F

F

V

V

Y

Y

F

Y

G

G

G

G

Y
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Y

H

Y

P

P

E

E

M

M

G

G

L

Q

I

M

M

L

R

R

Q

Q

A

A

R

R

E

S

Q

V

G

G

I

L

K

K

A

T

V

Q

F

F

M

M

G

G

P

P

E
|
E

G
|
G

V

V

G

G

N

N

K

A

D

S

I

L

T

S

A

N

I

I

A

A

G

G

P

D

A

A

S

A

E

E

G

G

M

M

L

L

V

V

T

T

L

M

P

P

A

K

D

R

F

Y

A

D

A

Q

D

D

P

P

A

A

N

N

A

Q

A

G

L

I

V

V

K

D

A

A

F

L

A

K

A

A

A

D

K

K

R

K

D

D

P

P

N

S

G

G

P

P

F
x
Y

Q

V

M

W

P

I

A

T

Y

Y

S

A

G

A

V

V

K

Q

I

S

I

L

A

A

D

T

A

A

I

L

A

E

G

R

A

T

K

G

S

S

T

D

D

E

P

P

E

L

K

A

V

L

A

V

A

K

Y

D

I

L

H

K

A

A

N

N

S

G

F

A

K

N

T

T

P

V

I

I

G

G

N

P

V

L

E

N

Y

W

D

D

K

E

K

K

G

G

D

D

L

L

K

K

S

G

F

F

K

D

F

F

V

G

V

V

F

F

T

Q

W

W

H

H

K

A

D

D

A

G

S

S

K

S

T

T

E

K

A

A

binding leucine: W18 (≠ Y44), L77 (≠ V103), C78 (= C104), S79 (= S105), G100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), Y202 (= Y229), E226 (= E253), G227 (= G254), Y276 (≠ F303)

1usiA L-leucine-binding protein with phenylalanine bound (see paper)
53% identity, 93% coverage: 27:372/373 of query aligns to 1:345/345 of 1usiA

query
sites
1usiA

E

D

T

D

V

I

K

K

I

V

A

A

I

V

A

V

G

G

P

A

M

M

T

S

G

G

A

P

V

I

A

A

Q

Q

Y
x
W

G

G

D

D

M

M

V

E

K

F

A

N

G

G

A

A

L

R

T

Q

A

A

I

I

E

K

Q

D

I

I

N

N

A

A

A

K

G

G

G

G

A

I

N

K

G

G

N

D

K

K

F

L

E

V

A

G

V

V

M

E

M

Y

D

D

D

D

A

A

C

C

E

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

I

V

V

S

N

Q

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

V
x
L

C
|
C

S
|
S

G

S

S

S

T

T

I

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

N

D

E

E

G

G

V

I

V

L

M

M

I

I

T

S

P

P

S
x
G

A
|
A

T
|
T

A

N

P

P

Q

E

L

L

T

T

E

Q

A

-

K

R

P

G

H

Y

K

Q

F

H

I

I

F

M

R

R

T

T

I

A

G

G

R

L

D

D

D

S

Q

S

Q

Q

G

G

P

P

A

T

A

A

A

A

R

K

Y

Y

V

I

I

L

E

E

K

T

L

V

K

K

P

P

K

Q

K

R

V

I

A

A

V

I

L

I

H

H

D

D

K

K

Q

Q

S

Q

Y
|
Y

G

G

Q

E

G

G

V

L

A

A

S

R

S

S

V

V

K

Q

A

D

A

G

L

L

D

K

A

A

A

A

K

N

V

A

P

N

V

V

V

V

V

F

F

F

E

D

G

G

I

I

N

T

A

A

G

G

D

E

S

K

D

D

Y

F

S

S

A

A

I

L

V

I

T

A

K

R

L

L

K

K

S

K

Q

E

G

N

V

I

D

D

F

F

V

V

Y

Y

F

Y

G

G

G

G

Y
|
Y

H

Y

P

P

E

E

M

M

G

G

L

Q

I

M

M

L

R

R

Q

Q

A

A

R

R

E

S

Q

V

G

G

I

L

K

K

A

T

V

Q

F

F

M

M

G

G

P

P

E
|
E

G
|
G

V

V

G

G

N

N

K

A

D

S

I

L

T

S

A

N

I

I

A

A

G

G

P

D

A

A

S

A

E

E

G

G

M

M

L

L

V

V

T

T

L

M

P

P

A

K

D

R

F

Y

A

D

A

Q

D

D

P

P

A

A

N

N

A

Q

A

G

L

I

V

V

K

D

A

A

F

L

A

K

A

A

A

D

K

K

R

K

D

D

P

P

N

S

G

G

P

P

F
x
Y

Q

V

M

W

P

I

A

T

Y

Y

S

A

G

A

V

V

K

Q

I

S

I

L

A

A

D

T

A

A

I

L

A

E

G

R

A

T

K

G

S

S

T

D

D

E

P

P

E

L

K

A

V

L

A

V

A

K

Y

D

I

L

H

K

A

A

N

N

S

G

F

A

K

N

T

T

P

V

I

I

G

G

N

P

V

L

E

N

Y

W

D

D

K

E

K

K

G

G

D

D

L

L

K

K

S

G

F

F

K

D

F

F

V

G

V

V

F

F

T

Q

W

W

H

H

K

A

D

D

A

G

S

S

K

S

T

T

E

K

A

A

binding phenylalanine: W18 (≠ Y44), L77 (≠ V103), C78 (= C104), S79 (= S105), G100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), Y202 (= Y229), E226 (= E253), G227 (= G254), Y276 (≠ F303)

1z18A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound valine (see paper)
52% identity, 93% coverage: 27:373/373 of query aligns to 1:344/344 of 1z18A

query
sites
1z18A

E

E

T

D

V

I

K

K

I

V

A

A

I

V

A

V

G

G

P

A

M

M

T

S

G

G

A

P

V

V

A

A

Q

Q

Y
|
Y

G

G

D

D

M

Q

V

E

K

F

A

T

G

G

A

A

L

E

T

Q

A

A

I

V

E

A

Q

D

I

I

N

N

A

A

A

K

G

G

G

G

A

I

N

K

G

G

N

N

K

K

F

L

E

Q

A

I

V

V

M

K

M

Y

D

D

D

D

A

A

C

C

E

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

V

V

V

S

N

Q

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

V
x
L

C
|
C

S
|
S

G

S

S

S

T

T

I

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

N

D

E

E

G

G

V

I

V

L

M

M

I

I

T

T

P

P

S
x
A

A
|
A

T
|
T

A

A

P

P

Q

E

L

L

T

T

E

-

A

A

K

R

P

G

H

Y

K

Q

F

L

I

I

F

L

R

R

T

T

I

T

G

G

R

L

D

D

D

S

Q

D

Q

Q

G

G

P

P

A

T

A

A

A

A

R

K

Y

Y

V

I

I

L

E

E

K

K

L

V

K

K

P

P

K

Q

K

R

V

I

A

A

V

I

L

V

H

H

D

D

K

K

Q

Q

S

Q

Y
|
Y

G

G

Q

E

G

G

V

L

A

A

S

R

S

A

V

V

K

Q

A

D

A

G

L

L

D

K

A

K

A

G

K

N

V

A

P

N

V

V

V

V

V

F

F

F

E

D

G

G

I

I

N

T

A

A

G

G

D

E

S

K

D

D

Y

F

S

S

A

T

I

L

V

V

T

A

K

R

L

L

K

K

S

K

Q

E

G

N

V

I

D

D

F

F

V

V

Y

Y

F

Y

G

G

G

G

Y
|
Y

H

H

P

P

E

E

M

M

G

G

L

Q

I

I

M

L

R

R

Q

Q

A

A

R

R

E

A

Q

A

G

G

I

L

K

K

A

T

V

Q

F

F

M

M

G

G

P

P

E
|
E

G

G

V

V

G

A

N

N

K

V

D

S

I

L

T

S

A

N

I

I

A

A

G

G

P

E

A

S

S

A

E

E

G

G

M

L

L

L

V

V

T

T

L

K

P

P

A

K

D

N

F

Y

A

D

A

Q

D

V

P

P

A

A

N

N

A

K

A

P

L

I

V

V

K

D

A

A

F

I

A

K

A

A

A

K

K

K

R

Q

D

D

P

P

N

S

G

G

P

A

F
|
F

Q

V

M

W

P

T

A

T

Y

Y

S

A

G

A

V

L

K

Q

I

S

I

L

A

Q

D

A

A

G

I

L

A

-

G

-

A

N

K

Q

S

S

T

D

D

D

P

P

E

A

K

E

V

I

A

A

A

K

Y

Y

I

L

H

K

A

A

N

N

S

S

F

V

K

D

T

T

P

V

I

M

G

G

N

P

V

L

E

T

Y

W

D

D

K

E

K

K

G

G

D

D

L

L

K

K

S

G

F

F

K

E

F

F

V

G

V

V

F

F

T

D

W

W

H

H

K

A

D

N

A

G

S

T

K

A

T

T

E

D

A

A

K

K

binding valine: Y18 (= Y44), L77 (≠ V103), C78 (= C104), S79 (= S105), A100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), Y202 (= Y229), E226 (= E253), F276 (= F303)

1z17A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound ligand isoleucine (see paper)
52% identity, 93% coverage: 27:373/373 of query aligns to 1:344/344 of 1z17A

query
sites
1z17A

E

E

T

D

V

I

K

K

I

V

A

A

I

V

A

V

G

G

P

A

M

M

T

S

G

G

A

P

V

V

A

A

Q

Q

Y
|
Y

G

G

D

D

M

Q

V

E

K

F

A

T

G

G

A

A

L

E

T

Q

A

A

I

V

E

A

Q

D

I

I

N

N

A

A

A

K

G

G

G

G

A

I

N

K

G

G

N

N

K

K

F

L

E

Q

A

I

V

V

M

K

M

Y

D

D

D

D

A

A

C

C

E

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

V

V

V

S

N

Q

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

V
x
L

C
|
C

S
|
S

G

S

S

S

T

T

I

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

N

D

E

E

G

G

V

I

V

L

M

M

I

I

T

T

P

P

S
x
A

A
|
A

T
|
T

A

A

P

P

Q

E

L

L

T

T

E

-

A

A

K

R

P

G

H

Y

K

Q

F

L

I

I

F

L

R

R

T

T

I

T

G

G

R

L

D

D

D

S

Q

D

Q

Q

G

G

P

P

A

T

A

A

A

A

R

K

Y

Y

V

I

I

L

E

E

K

K

L

V

K

K

P

P

K

Q

K

R

V

I

A

A

V

I

L

V

H

H

D

D

K

K

Q

Q

S

Q

Y
|
Y

G

G

Q

E

G

G

V

L

A

A

S

R

S

A

V

V

K

Q

A

D

A

G

L

L

D

K

A

K

A

G

K

N

V

A

P

N

V

V

V

V

V

F

F

F

E

D

G

G

I

I

N

T

A

A

G

G

D

E

S

K

D

D

Y

F

S

S

A

T

I

L

V

V

T

A

K

R

L

L

K

K

S

K

Q

E

G

N

V

I

D

D

F

F

V

V

Y

Y

F

Y

G

G

G

G

Y
|
Y

H

H

P

P

E

E

M

M

G

G

L

Q

I

I

M

L

R

R

Q

Q

A

A

R

R

E

A

Q

A

G

G

I

L

K

K

A

T

V

Q

F

F

M

M

G

G

P

P

E
|
E

G
|
G

V

V

G

A

N

N

K

V

D

S

I

L

T

S

A

N

I

I

A

A

G

G

P

E

A

S

S

A

E

E

G

G

M

L

L

L

V

V

T

T

L

K

P

P

A

K

D

N

F

Y

A

D

A

Q

D

V

P

P

A

A

N

N

A

K

A

P

L

I

V

V

K

D

A

A

F

I

A

K

A

A

A

K

K

K

R

Q

D

D

P

P

N

S

G

G

P

A

F
|
F

Q

V

M

W

P

T

A

T

Y

Y

S

A

G

A

V

L

K

Q

I

S

I

L

A

Q

D

A

A

G

I

L

A

-

G

-

A

N

K

Q

S

S

T

D

D

D

P

P

E

A

K

E

V

I

A

A

A

K

Y

Y

I

L

H

K

A

A

N

N

S

S

F

V

K

D

T

T

P

V

I

M

G

G

N

P

V

L

E

T

Y

W

D

D

K

E

K

K

G

G

D

D

L

L

K

K

S

G

F

F

K

E

F

F

V

G

V

V

F

F

T

D

W

W

H

H

K

A

D

N

A

G

S

T

K

A

T

T

E

D

A

A

K

K

binding isoleucine: Y18 (= Y44), L77 (≠ V103), C78 (= C104), S79 (= S105), A100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), Y202 (= Y229), E226 (= E253), G227 (= G254), F276 (= F303)

1z16A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound leucine (see paper)
52% identity, 93% coverage: 27:373/373 of query aligns to 1:344/344 of 1z16A

query
sites
1z16A

E

E

T

D

V

I

K

K

I

V

A

A

I

V

A

V

G

G

P

A

M

M

T

S

G

G

A

P

V

V

A

A

Q

Q

Y
|
Y

G

G

D

D

M

Q

V

E

K

F

A

T

G

G

A

A

L

E

T

Q

A

A

I

V

E

A

Q

D

I

I

N

N

A

A

A

K

G

G

G

G

A

I

N

K

G

G

N

N

K

K

F

L

E

Q

A

I

V

V

M

K

M

Y

D

D

D

D

A

A

C

C

E

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

V

V

V

S

N

Q

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

V
x
L

C
|
C

S
|
S

G

S

S

S

T

T

I

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

N

D

E

E

G

G

V

I

V

L

M

M

I

I

T

T

P

P

S
x
A

A
|
A

T
|
T

A

A

P

P

Q

E

L

L

T

T

E

-

A

A

K

R

P

G

H

Y

K

Q

F

L

I

I

F

L

R

R

T

T

I

T

G

G

R

L

D

D

D

S

Q

D

Q

Q

G

G

P

P

A

T

A

A

A

A

R

K

Y

Y

V

I

I

L

E

E

K

K

L

V

K

K

P

P

K

Q

K

R

V

I

A

A

V

I

L

V

H

H

D

D

K

K

Q

Q

S

Q

Y
|
Y

G

G

Q

E

G

G

V

L

A

A

S

R

S

A

V

V

K

Q

A

D

A

G

L

L

D

K

A

K

A

G

K

N

V

A

P

N

V

V

V

V

V

F

F

F

E

D

G

G

I

I

N

T

A

A

G

G

D

E

S

K

D

D

Y

F

S

S

A

T

I

L

V

V

T

A

K

R

L

L

K

K

S

K

Q

E

G

N

V

I

D

D

F

F

V

V

Y

Y

F

Y

G

G

G

G

Y
|
Y

H

H

P

P

E

E

M

M

G

G

L

Q

I

I

M

L

R

R

Q

Q

A

A

R

R

E

A

Q

A

G

G

I

L

K

K

A

T

V

Q

F

F

M

M

G

G

P

P

E
|
E

G
|
G

V

V

G

A

N

N

K

V

D

S

I

L

T

S

A

N

I

I

A

A

G

G

P

E

A

S

S

A

E

E

G

G

M

L

L

L

V

V

T

T

L

K

P

P

A

K

D

N

F

Y

A

D

A

Q

D

V

P

P

A

A

N

N

A

K

A

P

L

I

V

V

K

D

A

A

F

I

A

K

A

A

A

K

K

K

R

Q

D

D

P

P

N

S

G

G

P

A

F
|
F

Q

V

M

W

P

T

A

T

Y

Y

S

A

G

A

V

L

K

Q

I

S

I

L

A

Q

D

A

A

G

I

L

A

-

G

-

A

N

K

Q

S

S

T

D

D

D

P

P

E

A

K

E

V

I

A

A

A

K

Y

Y

I

L

H

K

A

A

N

N

S

S

F

V

K

D

T

T

P

V

I

M

G

G

N

P

V

L

E

T

Y

W

D

D

K

E

K

K

G

G

D

D

L

L

K

K

S

G

F

F

K

E

F

F

V

G

V

V

F

F

T

D

W

W

H

H

K

A

D

N

A

G

S

T

K

A

T

T

E

D

A

A

K

K

binding leucine: Y18 (= Y44), L77 (≠ V103), C78 (= C104), S79 (= S105), A100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), Y202 (= Y229), E226 (= E253), G227 (= G254), F276 (= F303)

3ip9A Structure of atu2422-gaba receptor in complex with gaba (see paper)
44% identity, 90% coverage: 29:365/373 of query aligns to 3:337/348 of 3ip9A

query
sites
3ip9A

V

V

K

V

I

I

A

A

I

V

A

G

G

A

P

P

M

L

T

T

G

G

A

P

V

N

A

A

Q

A

Y

F

G

G

D

A

M

Q

V

I

K

Q

A

K

G

G

A

A

L

E

T

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

A

A

G

G

G

G

A

I

N

N

G

G

N

E

K

Q

F

I

E

K

A

I

V

V

M

L

M

G

D

D

D

D

A

V

C

S

E

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

S

A

Q

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

V
x
F

C
x
N

S
|
S

G

G

S

V

T

S

I

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

N

E

E

N

G

G

V

I

V

L

M

E

I

I

T

T

P

P

S
x
A

A
|
A

T
|
T

A

N

P

P

Q

V

L

F

T

T

E

E

A

R

K

G

P

L

H

W

K

N

F

-

I

T

F

F

R

R

T

T

I

C

G

G

R

R

D

D

D

D

Q

Q

Q

Q

G

G

P

G

A

I

A

A

A

G

R

K

Y

Y

V

L

I

A

E

D

K

H

L

F

K

K

P

D

K

A

K

K

V

V

A

A

V

I

L

I

H

H

D

D

K

K

Q

T

S

P

Y
|
Y

G

G

Q

Q

G

G

V

L

A

A

S

D

S

E

V

T

K

K

A

K

A

A

L

A

D

N

A

A

A

A

K

G

V

V

P

T

V

E

V

V

V

M

F

Y

E

E

G

G

I

V

N

N

A

V

G

G

D

D

S

K

D

D

Y

F

S

S

A

A

I

L

V

I

T

S

K

K

L

M

K

K

S

E

Q

A

G

G

V

V

D

S

F

I

V

I

Y

Y

F

W

G

G

G

G

Y

L

H

H

P

T

E

E

M

A

G

G

L

L

I

I

M

I

R

R

Q

Q

A

A

R

A

E

D

Q

Q

G

G

I

L

K

K

A

A

V

K

F

L

M

V

G

S

P

G

E
x
D

G
|
G

V

I

G

V

N

S

K

N

D

E

I

L

T

A

A

S

I

I

A

A

G

G

P

D

A

A

S

V

E

E

G

G

M

T

L

L

V

N

T

T

L

F

P

G

A

P

D

D

F

P

A

T

A

L

D

R

P

P

A

E

N

N

A

K

A

E

L

L

V

V

K

E

A

K

F

F

A

K

A

A

A

A

K

G

R

F

D

N

P

P

N

E

G

A

P

-

F
x
Y

Q

T

M

L

P

Y

A

S

Y

Y

S

A

G

A

V

M

K

Q

I

A

I

I

A

A

D

G

A

A

I

A

A

K

G

A

A

A

K

G

S

S

T

V

D

E

P

P

E

E

K

K

V

V

A

A

A

E

Y

A

I

L

H

K

A

K

N

G

S

S

F

F

K

P

T

T

P

A

I

L

G

G

N

E

V

I

E

S

Y

F

D

D

K

E

K

K

G

G

D

D

L

P

K

K

S

L

F

P

K

G

F

Y

V

V

V

M

F

Y

T

E

W

W

H

K

K

K

binding gamma-amino-butanoic acid: F77 (≠ V103), N78 (≠ C104), S79 (= S105), A100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), D226 (≠ E253), G227 (= G254), Y275 (≠ F303)

3ip7A Structure of atu2422-gaba receptor in complex with valine (see paper)
44% identity, 90% coverage: 29:365/373 of query aligns to 3:337/348 of 3ip7A

query
sites
3ip7A

V

V

K

V

I

I

A

A

I

V

A

G

G

A

P

P

M

L

T

T

G

G

A

P

V

N

A

A

Q

A

Y

F

G

G

D

A

M

Q

V

I

K

Q

A

K

G

G

A

A

L

E

T

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

A

A

G

G

G

G

A

I

N

N

G

G

N

E

K

Q

F

I

E

K

A

I

V

V

M

L

M

G

D

D

D

D

A

V

C

S

E

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

S

A

Q

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

V
x
F

C
x
N

S
|
S

G
|
G

S

V

T

S

I

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

N

E

E

N

G

G

V

I

V

L

M

E

I

I

T

T

P

P

S
x
A

A
|
A

T
|
T

A

N

P

P

Q

V

L

F

T

T

E

E

A

R

K

G

P

L

H

W

K

N

F

-

I

T

F

F

R

R

T

T

I

C

G

G

R

R

D

D

D

D

Q

Q

Q

Q

G

G

P

G

A

I

A

A

A

G

R

K

Y

Y

V

L

I

A

E

D

K

H

L

F

K

K

P

D

K

A

K

K

V

V

A

A

V

I

L

I

H

H

D

D

K

K

Q
x
T

S
x
P

Y
|
Y

G

G

Q

Q

G

G

V

L

A

A

S

D

S

E

V

T

K

K

A

K

A

A

L

A

D

N

A

A

A

A

K

G

V

V

P

T

V

E

V

V

V

M

F

Y

E

E

G

G

I

V

N

N

A

V

G

G

D

D

S

K

D

D

Y

F

S

S

A

A

I

L

V

I

T

S

K

K

L

M

K

K

S

E

Q

A

G

G

V

V

D

S

F

I

V

I

Y

Y

F

W

G

G

G

G

Y
x
L

H

H

P

T

E

E

M

A

G

G

L

L

I

I

M

I

R

R

Q

Q

A

A

R

A

E

D

Q

Q

G

G

I

L

K

K

A

A

V

K

F

L

M

V

G

S

P

G

E
x
D

G
|
G

V

I

G

V

N

S

K

N

D

E

I

L

T

A

A

S

I

I

A

A

G

G

P

D

A

A

S

V

E

E

G

G

M

T

L

L

V

N

T

T

L

F

P

G

A

P

D

D

F

P

A

T

A

L

D

R

P

P

A

E

N

N

A

K

A

E

L

L

V

V

K

E

A

K

F

F

A

K

A

A

A

A

K

G

R

F

D

N

P

P

N

E

G

A

P

-

F

Y

Q

T

M

L

P

Y

A

S

Y

Y

S

A

G

A

V

M

K

Q

I

A

I

I

A

A

D

G

A

A

I

A

A

K

G

A

A

A

K

G

S

S

T

V

D

E

P

P

E

E

K

K

V

V

A

A

A

E

Y

A

I

L

H
x
K

A

K

N

G

S

S

F

F

K

P

T

T

P

A

I

L

G

G

N

E

V

I

E

S

Y
x
F

D

D

K

E

K

K

G

G

D

D

L

P

K
|
K

S

L

F

P

K

G

F

Y

V

V

V

M

F

Y

T

E

W

W

H

K

K

K

binding calcium ion: S79 (= S105), G80 (= G106), T148 (≠ Q175), P149 (≠ S176), Y150 (= Y177), K306 (≠ H334), F319 (≠ Y347), K326 (= K354)
binding valine: F77 (≠ V103), N78 (≠ C104), S79 (= S105), A100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), L202 (≠ Y229), D226 (≠ E253), G227 (= G254)

3ip6A Structure of atu2422-gaba receptor in complex with proline (see paper)
44% identity, 90% coverage: 29:365/373 of query aligns to 3:337/348 of 3ip6A

query
sites
3ip6A

V

V

K

V

I

I

A

A

I

V

A

G

G

A

P

P

M

L

T

T

G

G

A

P

V

N

A

A

Q

A

Y

F

G

G

D

A

M

Q

V

I

K

Q

A

K

G

G

A

A

L

E

T

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

A

A

G

G

G

G

A

I

N

N

G

G

N

E

K

Q

F

I

E

K

A

I

V

V

M

L

M

G

D

D

D

D

A

V

C

S

E

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

S

A

Q

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

V
x
F

C
x
N

S
|
S

G

G

S

V

T

S

I

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

N

E

E

N

G

G

V

I

V

L

M

E

I

I

T

T

P

P

S
x
A

A
|
A

T
|
T

A

N

P

P

Q

V

L

F

T

T

E

E

A

R

K

G

P

L

H

W

K

N

F

-

I

T

F

F

R

R

T

T

I

C

G

G

R

R

D

D

D

D

Q

Q

Q

Q

G

G

P

G

A

I

A

A

A

G

R

K

Y

Y

V

L

I

A

E

D

K

H

L

F

K

K

P

D

K

A

K

K

V

V

A

A

V

I

L

I

H

H

D

D

K

K

Q

T

S

P

Y
|
Y

G

G

Q

Q

G

G

V

L

A

A

S

D

S

E

V

T

K

K

A

K

A

A

L

A

D

N

A

A

A

A

K

G

V

V

P

T

V

E

V

V

V

M

F

Y

E

E

G

G

I

V

N

N

A

V

G

G

D

D

S

K

D

D

Y

F

S

S

A

A

I

L

V

I

T

S

K

K

L

M

K

K

S

E

Q

A

G

G

V

V

D

S

F

I

V

I

Y

Y

F

W

G

G

G

G

Y

L

H

H

P

T

E

E

M

A

G

G

L

L

I

I

M

I

R

R

Q

Q

A

A

R

A

E

D

Q

Q

G

G

I

L

K

K

A

A

V

K

F

L

M

V

G

S

P

G

E
x
D

G
|
G

V

I

G

V

N

S

K

N

D

E

I

L

T

A

A

S

I

I

A

A

G

G

P

D

A

A

S

V

E

E

G

G

M

T

L

L

V

N

T

T

L

F

P

G

A

P

D

D

F

P

A

T

A

L

D

R

P

P

A

E

N

N

A

K

A

E

L

L

V

V

K

E

A

K

F

F

A

K

A

A

A

A

K

G

R

F

D

N

P

P

N

E

G

A

P

-

F
x
Y

Q

T

M

L

P

Y

A

S

Y

Y

S

A

G

A

V

M

K

Q

I

A

I

I

A

A

D

G

A

A

I

A

A

K

G

A

A

A

K

G

S

S

T

V

D

E

P

P

E

E

K

K

V

V

A

A

A

E

Y

A

I

L

H

K

A

K

N

G

S

S

F

F

K

P

T

T

P

A

I

L

G

G

N

E

V

I

E

S

Y

F

D

D

K

E

K

K

G

G

D

D

L

P

K

K

S

L

F

P

K

G

F

Y

V

V

V

M

F

Y

T

E

W

W

H

K

K

K

binding proline: F77 (≠ V103), N78 (≠ C104), S79 (= S105), A100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), D226 (≠ E253), G227 (= G254), Y275 (≠ F303)

3ip5A Structure of atu2422-gaba receptor in complex with alanine (see paper)
44% identity, 90% coverage: 29:365/373 of query aligns to 3:337/348 of 3ip5A

query
sites
3ip5A

V

V

K

V

I

I

A

A

I

V

A

G

G

A

P

P

M

L

T

T

G

G

A

P

V

N

A

A

Q

A

Y

F

G

G

D

A

M

Q

V

I

K

Q

A

K

G

G

A

A

L

E

T

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

A

A

G

G

G

G

A

I

N

N

G

G

N

E

K

Q

F

I

E

K

A

I

V

V

M

L

M

G

D

D

D

D

A

V

C

S

E

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

S

A

Q

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

V
x
F

C
x
N

S
|
S

G

G

S

V

T

S

I

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

N

E

E

N

G

G

V

I

V

L

M

E

I

I

T

T

P

P

S
x
A

A
|
A

T
|
T

A

N

P

P

Q

V

L

F

T

T

E

E

A

R

K

G

P

L

H

W

K

N

F

-

I

T

F

F

R

R

T

T

I

C

G

G

R

R

D

D

D

D

Q

Q

Q

Q

G

G

P

G

A

I

A

A

A

G

R

K

Y

Y

V

L

I

A

E

D

K

H

L

F

K

K

P

D

K

A

K

K

V

V

A

A

V

I

L

I

H

H

D

D

K

K

Q

T

S

P

Y
|
Y

G

G

Q

Q

G

G

V

L

A

A

S

D

S

E

V

T

K

K

A

K

A

A

L

A

D

N

A

A

A

A

K

G

V

V

P

T

V

E

V

V

V

M

F

Y

E

E

G

G

I

V

N

N

A

V

G

G

D

D

S

K

D

D

Y

F

S

S

A

A

I

L

V

I

T

S

K

K

L

M

K

K

S

E

Q

A

G

G

V

V

D

S

F

I

V

I

Y

Y

F

W

G

G

G

G

Y

L

H

H

P

T

E

E

M

A

G

G

L

L

I

I

M

I

R

R

Q

Q

A

A

R

A

E

D

Q

Q

G

G

I

L

K

K

A

A

V

K

F

L

M

V

G

S

P

G

E
x
D

G

G

V

I

G

V

N

S

K

N

D

E

I

L

T

A

A

S

I

I

A

A

G

G

P

D

A

A

S

V

E

E

G

G

M

T

L

L

V

N

T

T

L

F

P

G

A

P

D

D

F

P

A

T

A

L

D

R

P

P

A

E

N

N

A

K

A

E

L

L

V

V

K

E

A

K

F

F

A

K

A

A

A

A

K

G

R

F

D

N

P

P

N

E

G

A

P

-

F

Y

Q

T

M

L

P

Y

A

S

Y

Y

S

A

G

A

V

M

K

Q

I

A

I

I

A

A

D

G

A

A

I

A

A

K

G

A

A

A

K

G

S

S

T

V

D

E

P

P

E

E

K

K

V

V

A

A

A

E

Y

A

I

L

H

K

A

K

N

G

S

S

F

F

K

P

T

T

P

A

I

L

G

G

N

E

V

I

E

S

Y

F

D

D

K

E

K

K

G

G

D

D

L

P

K

K

S

L

F

P

K

G

F

Y

V

V

V

M

F

Y

T

E

W

W

H

K

K

K

binding alanine: F77 (≠ V103), N78 (≠ C104), S79 (= S105), A100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), D226 (≠ E253)

3ipcA Structure of atu2422-gaba f77a mutant receptor in complex with leucine (see paper)
44% identity, 90% coverage: 29:365/373 of query aligns to 3:337/348 of 3ipcA

query
sites
3ipcA

V

V

K

V

I

I

A

A

I

V

A

G

G

A

P

P

M

L

T

T

G

G

A

P

V

N

A

A

Q

A

Y
x
F

G

G

D

A

M

Q

V

I

K

Q

A

K

G

G

A

A

L

E

T

Q

A

A

I

A

E

K

Q

D

I

I

N

N

A

A

A

A

G

G

G

G

A

I

N

N

G

G

N

E

K

Q

F

I

E

K

A

I

V

V

M

L

M

G

D

D

D

D

A

V

C

S

E

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

S

A

Q

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

V
x
A

C
x
N

S
|
S

G

G

S

V

T

S

I

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

N

E

E

N

G

G

V

I

V

L

M

E

I

I

T

T

P

P

S
x
A

A
|
A

T
|
T

A

N

P

P

Q

V

L

F

T

T

E

E

A

-

K

R

P

G

H

L

K

W

F

N

I

T

F

F

R

R

T

T

I

C

G

G

R

R

D

D

D

D

Q

Q

Q

Q

G

G

P

G

A

I

A

A

A

G

R

K

Y

Y

V

L

I

A

E

D

K

H

L

F

K

K

P

D

K

A

K

K

V

V

A

A

V

I

L

I

H

H

D

D

K

K

Q

T

S

P

Y
|
Y

G

G

Q

Q

G

G

V

L

A

A

S

D

S

E

V

T

K

K

A

K

A

A

L

A

D

N

A

A

A

A

K

G

V

V

P

T

V

E

V

V

V

M

F

Y

E

E

G

G

I

V

N

N

A

V

G

G

D

D

S

K

D

D

Y

F

S

S

A

A

I

L

V

I

T

S

K

K

L

M

K

K

S

E

Q

A

G

G

V

V

D

S

F

I

V

I

Y

Y

F

W

G

G

G

G

Y

L

H

H

P

T

E

E

M

A

G

G

L

L

I

I

M

I

R

R

Q

Q

A

A

R

A

E

D

Q

Q

G

G

I

L

K

K

A

A

V

K

F

L

M

V

G

S

P

G

E
x
D

G
|
G

V

I

G

V

N

S

K

N

D

E

I

L

T

A

A

S

I

I

A

A

G

G

P

D

A

A

S

V

E

E

G

G

M

T

L

L

V

N

T

T

L

F

P

G

A

P

D

D

F

P

A

T

A

L

D

R

P

P

A

E

N

N

A

K

A

E

L

L

V

V

K

E

A

K

F

F

A

K

A

A

A

A

K

G

R

F

D

N

P

P

N

E

G

A

P

-

F
x
Y

Q

T

M

L

P

Y

A

S

Y

Y

S

A

G

A

V

M

K

Q

I

A

I

I

A

A

D

G

A

A

I

A

A

K

G

A

A

A

K

G

S

S

T

V

D

E

P

P

E

E

K

K

V

V

A

A

A

E

Y

A

I

L

H

K

A

K

N

G

S

S

F

F

K

P

T

T

P

A

I

L

G

G

N

E

V

I

E

S

Y

F

D

D

K

E

K

K

G

G

D

D

L

P

K

K

S

L

F

P

K

G

F

Y

V

V

V

M

F

Y

T

E

W

W

H

K

K

K

binding leucine: F18 (≠ Y44), A77 (≠ V103), N78 (≠ C104), S79 (= S105), A100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), D226 (≠ E253), G227 (= G254), Y275 (≠ F303)

4n0qB Crystal structure of an abc transporter, substrate-binding protein from brucella melitensis 16m in complex with l-leucine using a crystal grown in a crystal former (microlytic)
45% identity, 90% coverage: 29:363/373 of query aligns to 3:337/345 of 4n0qB

query
sites
4n0qB

V

I

K

T

I

I

A

G

I

V

A

I

G

A

P

P

M

L

T

T

G

G

A

P

V

V

A

A

Q

A

Y
x
F

G

G

D

D

M

Q

V

V

K

K

A

K

G

G

A

A

L

E

T

T

A

A

I

V

E

E

Q

V

I

I

N

N

A

K

A

A

G

G

G

G

A

I

N

K

G

G

N

E

K

K

F

V

E

V

A

L

V

K

M

F

M

A

D

D

D

D

A

A

C

G

E

E

P

P

K

K

Q

Q

A

G

V

V

A

S

V

A

A

A

N

N

K

Q

I

I

V

V

S

G

Q

D

G

G

I

I

K

K

Y

F

V

V

I

V

G

G

H

L

V
|
V

C
x
T

S
x
T

G

G

S

V

T

A

I

V

P

P

A

V

S

S

D

D

I

V

Y

L

E

S

N

E

E

N

G

G

V

V

V

L

M

M

I

V

T

T

P

P

S
x
T

A
|
A

T
|
T

A

G

P

P

Q

D

L

L

T

T

E

-

A

A

K

R

P

G

H

L

K

E

F

N

I

V

F

F

R

R

T

T

I

C

G

G

R

R

D

D

D

G

Q

Q

Q

Q

G

A

P

E

A

V

A

M

A

A

R

D

Y

Y

V

V

I

L

E

K

K

N

L

M

K

K

P

D

K

K

K

K

V

V

A

A

V

V

L

I

H

H

D

D

K

K

Q

G

S

A

Y
|
Y

G

G

Q

K

G

G

V

L

A

A

S

D

S

A

V

F

K

K

A

A

A

A

L

I

D

N

A

K

A

G

K

G

V

I

P

T

V

E

V

V

V

H

F

Y

E

D

G

S

I

V

N

T

A

P

G

G

D

D

S

K

D

D

Y

F

S

S

A

A

I

L

V

V

T

T

K

K

L

L

K

K

S

S

Q

A

G

G

V

A

D

E

F

V

V

V

Y

Y

F

F

G

G

G

G

Y
|
Y

H

H

P

A

E

E

M

G

G

G

L

L

I

L

M

S

R

R

Q

Q

A

L

R

H

E

D

Q

A

G

G

I

M

K

Q

A

A

V

L

F

V

M

L

G

G

P

G

E
|
E

G
|
G

V

L

G

S

N

N

K

T

D

E

I

Y

T

W

A

A

I

I

A

G

G

G

P

T

A

N

S

A

E

Q

G

G

M

T

L

L

V

F

T

T

L

N

P

A

A

K

D

D

F

A

A

T

A

K

D

N

P

P

A

A

N

A

A

K

A

D

L

A

V

I

K

Q

A

A

F

L

A

-

A

K

A

A

K

K

R

N

D

I

P

P

N

A

G

E

P

A

F
|
F

Q

T

M

M

P

N

A

A

Y

Y

S

A

G

A

V

V

K

E

I

V

I

I

A

K

D

A

A

G

I

I

A

E

G

R

A

A

K

G

S

S

T

T

D

D

P

D

E

S

-

A

K

A

V

V

A

A

A

K

Y

A

I

L

H

H

-

D

A

G

N

K

S

P

F

I

K

E

T

T

P

A

I

I

G

G

N

T

V

L

E

T

Y

Y

D

S

K

E

K

T

G

G

D

D

L

L

K

S

S

S

F

P

K

S

F

F

V

D

V

I

F

F

T

K

W

W

binding leucine: F18 (≠ Y44), V77 (= V103), T78 (≠ C104), T79 (≠ S105), T100 (≠ S126), A101 (= A127), T102 (= T128), Y150 (= Y177), Y202 (= Y229), E226 (= E253), G227 (= G254), F275 (= F303)

4gnrA 1.0 angstrom resolution crystal structure of the branched-chain amino acid transporter substrate binding protein livj from streptococcus pneumoniae str. Canada mdr_19a in complex with isoleucine
33% identity, 80% coverage: 27:324/373 of query aligns to 1:298/348 of 4gnrA

query
sites
4gnrA

E

K

T

T

V

I

K

K

I

I

A

G

I

F

A

N

G

F

P

E

M

E

T

S

G

G

A

S

V

L

A

A

Q

A

Y
|
Y

G

G

D

T

M

A

V

E

K

Q

A

K

G

G

A

A

L

Q

T

L

A

A

I

V

E

D

Q

E

I

I

N

N

A

A

A

A

G

G

G

G

A

I

N

D

G

G

N

K

K

Q

F

I

E

E

A

V

V

V

M

D

M

K

D

D

D

N

A

K

C

S

E

E

P

T

K

A

Q

E

A

A

V

A

A

S

V

V

A

T

N

T

K

N

I

L

V

V

S

T

Q

Q

G

S

-

K

I

V

K

S

Y

A

V

V

I

V

G

G

H

P

V
x
A

C
x
T

S
|
S

G

G

S

A

T

T

I

A

P

A

A

A

S

V

D

A

I

N

Y

A

E

T

N

K

E

A

G

G

V

V

V

P

M

L

I

I

T

S

P

P

S
|
S

A
|
A

T
|
T

A

Q

P

D

Q

G

L

L

T

T

E

-

A

-

K

K

P

G

H

Q

K

D

F

Y

I

L

F

F

R

I

T

G

I

T

G

F

R

Q

D

D

D

S

Q

F

Q

Q

G

G

P

K

A

I

A

I

A

S

R

N

Y

Y

V

V

I

S

E

E

K

K

L

L

K

N

P

A

K

K

K

K

V

V

A

V

V

L

L

Y

H

T

D

D

K

N

Q

A

S

S

-

D

Y
|
Y

G

A

Q

K

G

G

V

I

A

A

S

K

S

S

V

F

K

R

A

-

A

-

L

-

D

E

A

S

A

Y

K

K

V

G

P

E

V

I

V

V

V

A

F

D

E

E

G

T

I

F

N

V

A

A

G

G

D

D

S

T

D

D

Y

F

S

Q

A

A

I

A

V

L

T

T

K

K

L

M

K

K

S

G

Q

K

G

D

V

F

D

D

F

A

V

I

Y

V

F

V

G

P

G

G

Y
|
Y

H

Y

P

N

E

E

M

A

G

G

L

K

I

I

M

V

R

N

Q

Q

A

A

R

R

E

G

Q

M

G

G

I

I

K

D

A

K

V

P

F

I

M

V

G

G

P

G

E
x
D

G
|
G

V

F

G

N

N

G

K

E

D

E

I

F

T

V

A

Q

I

Q

A

A

G

-

P

T

A

A

S

E

E

K

G

A

M

S

L

N

V

I

T

Y

L

F

P

I

A

S

D

G

F

F

A

S

A

T

D

T

P

V

A

E

N

V

A

S

A

A

L

K

V

A

K

K

A

A

F

F

A

L

A

D

A

A

K

Y

R

R

-

A

-

K

-

Y

-

N

-

E

D

E

P

P

N

S

G

-

P

T

F
|
F

Q

A

M

A

P

L

A

A

Y

Y

S

D

G

S

V

V

K

H

I

L

I

V

A

A

D

N

A

A

I

A

A

K

G

G

A

A

K

K

S

N

T

S

binding isoleucine: Y18 (= Y44), A78 (≠ V103), T79 (≠ C104), S80 (= S105), S101 (= S126), A102 (= A127), T103 (= T128), Y151 (= Y177), Y200 (= Y229), D224 (≠ E253), G225 (= G254), F277 (= F303)

3td9A Crystal structure of a leucine binding protein livk (tm1135) from thermotoga maritima msb8 at 1.90 a resolution
31% identity, 91% coverage: 28:368/373 of query aligns to 1:338/350 of 3td9A

query
sites
3td9A

T

V

V

V

K

K

I

I

A

A

I

V

A

I

G

L

P

P

M

M

T

T

G

G

A

G

V

I

A

S

Q

A

Y
x
F

G

G

D

R

M

M

V

V

K

W

A

E

G

G

A

I

L

Q

T

I

A

A

I

H

E

E

Q

E

I

-

N

-

A

-

A

K

G

P

G

T

A

V

N

L

G

G

N

E

K

E

F

V

E

E

A

L

V

V

M

L

M

L

D

D

D

T

A

R

C

S

E

E

P

K

K

T

Q

E

A

A

V

A

A

N

V

A

A

A

N

A

K

R

I

A

V

I

S

D

-

K

Q

E

G

K

I

V

K

L

Y

A

V

I

I

I

G

G

H

E

V
|
V

C
x
A

S
|
S

G

A

S

H

T

S

I

L

P

A

A

I

S

A

D

P

I

I

Y

A

E

E

N

E

E

N

G

K

V

V

V

P

M

M

I

V

T

T

P

P

S
x
A

A
x
S

T
|
T

A

N

P

P

Q

L

L

V

T

T

E

Q

A

G

K

R

P

-

H

-

K

K

F

F

I

V

F

S

R

R

T

V

I

C

G

F

R

I

D

D

D

P

Q

F

Q

Q

G

G

P

A

A

A

A

M

A

A

R

V

Y

F

V

A

I

Y

E

K

K

N

L

L

K

G

P

A

K

K

K

R

V

V

A

V

V

V

L

F

H

T

D

D

-

V

K

E

Q

Q

S

D

Y
|
Y

G

S

Q

V

G

G

V

L

A

S

S

N

S

F

V

F

-

I

-

N

K

K

A

F

A

T

L

E

D

L

A

G

A

G

K

Q

V

V

P

K

V

R

V

V

V

F

F

F

E

R

G

-

I

-

N

-

A

S

G

G

D

D

S

Q

D

D

Y

F

S

S

A

A

I

Q

V

L

T

S

K

V

L

A

K

M

S

S

Q

F

G

N

V

P

D

D

F

A

V

I

Y

Y

F

I

G

T

G

G

Y
|
Y

H

Y

P

P

E

E

M

I

G

A

L

L

I

I

M

S

R

R

Q

Q

A

A

R

R

E

Q

Q

L

G

G

I

F

K

T

A

G

V

Y

F

I

M

L

G

A

P

G

E
x
D

G
|
G

V

A

G

D

N

A

K

P

D

E

I

L

T

I

A

E

I

I

A

G

G

G

P

E

A

A

S

V

E

E

G

G

M

L

L

L

V

F

T

T

L

-

P

-

A

T

D
x
H

F

Y

A

H

A

P

D

K

P

A

A

A

N

S

A

N

A

P

L

V

V

A

K

K

A

K

F

F

A

V

A

E

A

V

K

Y

R

K

D

E

P

K

N

Y

G

G

-

K

-

E

P

P

F

A

Q

A

M
x
L

P

N

A

A

-

L

-

G

Y

Y

S

D

G

A

V

Y

K

M

I

V

I

L

A

L

D

D

A

A

I

I

A

E

G

R

A

A

K

G

S

S

T

F

D

D

P

R

E

E

K

K

V

I

A

A

A

E

Y

E

I

I

H

R

-

K

A

T

N

R

S

N

F

F

K

N

T

G

P

A

I

S

G

G

N

I

V

I

E

N

Y

I

D

D

K

E

K

N

G

G

D

D

L

-

K

-

S

A

F

I

K

K

F

S

V

V

V

V

F

V

T

N

W

I

H

V

K

K

D

N

A

G

S

S

binding phenylalanine: F17 (≠ Y44), V74 (= V103), A75 (≠ C104), S76 (= S105), A97 (≠ S126), S98 (≠ A127), T99 (= T128), Y147 (= Y177), Y198 (= Y229), D222 (≠ E253), G223 (= G254), H245 (≠ D278), L274 (≠ M305)

4mlcA Abc transporter substrate-binding protein fromdesulfitobacterium hafniense
27% identity, 87% coverage: 27:352/373 of query aligns to 1:317/336 of 4mlcA

query
sites
4mlcA

E

E

T

E

V

I

K

F

I

I

A

G

I

V

A

A

G

W

P

P

M

F

T

-

G

-

A

-

V

-

A

A

Q

S

Y

L

G

D

D

D

M

L

V

F

K

A

A

E

G

G

A

L

L

E

T

L

A

A

I

V

E

Q

Q

E

I

I

N

N

A

E

A

Q

G

G

G

G

A

V

N

Q

G

G

N

R

K

K

F

L

E

S

A

L

V

V

M

K

M

A

D

D

D

D

A

E

C

A

E

E

P

L

K

E

Q

K

A

G

V

L

A

A

V

I

A

A

N

Q

K

A

I

F

V

A

S

D

Q

N

-

A

G

G

I

I

K

Q

Y

A

V

V

I

I

G

G