SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS08660 FitnessBrowser__HerbieS:HSERO_RS08660 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

C1DFH7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
72% identity, 100% coverage: 1:338/338 of query aligns to 1:338/338 of C1DFH7

query
sites
C1DFH7

M

M

K

K

V

V

F

Y

Y

Y

D

D

K

K

D

D

A

C

D

D

L

L

S

S

L

I

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N

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K

K

N

K

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T

A

I

I

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G

Y

Y

G

G

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S

Q

Q

G

G

H

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A

A

H

H

A

A

L

C

N

N

L

L

K

K

D

D

S

S

G

G

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V

K

D

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Y

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V

G

G

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L

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R

K

A

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G

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A

A

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S

W

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N

A

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K

A

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E

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A

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K

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L

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F

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G

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A

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K

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D

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A

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M

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Y

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E

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Y

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E

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I

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M

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Y

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M

M

T

S

A

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Y

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R

I

N

N

N

A

A

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A

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H

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Q

I

I

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E

I

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G

G

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E

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I

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A

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I

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V

D

D

Q

K

S

T

K

K

N

N

D190 (= D190) binding Mg(2+)
E226 (= E226) binding Mg(2+)
E230 (= E230) binding Mg(2+)

4xiyA Crystal structure of ketol-acid reductoisomerase from azotobacter (see paper)
73% identity, 97% coverage: 1:327/338 of query aligns to 1:327/328 of 4xiyA

query
sites
4xiyA

M

M

K

K

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V

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Y

Y

Y

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D

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K

D

D

A

C

D

D

L

L

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L

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I

I

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K

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K

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V

T

A

I

I

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G

Y

Y

G

G

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S

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Q

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G

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H

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A

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A

A

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C

N

N

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L

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K

D

D

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S

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G

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K

D

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Y

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A

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A

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A

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E

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D

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A

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D

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M

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P

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D

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K

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D

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D

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K

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A

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A

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L

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F

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E
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A

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L

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C

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V

E

E

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L

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K

A

A

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A

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M

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A

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E

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L

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V

D

D

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Y

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E

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G

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I

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A

N

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M

M

N

N

Y

Y

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S

I

I

S

S

N

N

A

A

E

E

Y

Y

G

G

E

E

Y

Y

V

V

T

T

G

G

P

P

R

E

V

V

I

I

N

N

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E

E

Q

S

S

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R

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A

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M

K

R

K

N

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M

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A

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Q

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G

G

E

E

K

K

L

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M

M

P

P

W

W

I

I

active site: K130 (= K130), D190 (= D190), E194 (= E194)
binding fe (iii) ion: D190 (= D190), E194 (= E194)
binding magnesium ion: E226 (= E226), E230 (= E230)

8upqB Campylobacter jejuni ketol-acid reductoisomerase in complex with 2,3- dihydroxy-3-isovalerate.
64% identity, 100% coverage: 1:338/338 of query aligns to 2:339/342 of 8upqB

query
sites
8upqB

M

I

K

T

V

V

F

Y

Y

Y

D

D

K

K

D

D

A

C

D

D

L

L

S

N

L

L

I

I

K

K

N

S

K

K

N

K

V

V

T

A

I

I

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G

Y

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G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

V

V

K

N

V

V

T

T

V

I

G

G

L

L

R

R

K

E

G

G

G

S

A

V

S

S

W

A

N

V

K

K

A

A

E

K

Q

N

A

A

G

G

L

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K

E

V

V

A

M

E

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S

E

E

A

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K

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I

A

A

D

D

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V

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I

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M

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I

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P

D

D

E

E

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E

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G

A

K

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A

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A

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F

A

A

H

H

G

G

F

F

N

N

I

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Y

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G

Q

Q

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I

V

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R

P

A

K

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G

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S

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A

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L

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Y

A

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S

A

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T

N

T

F

F

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K

E

A

E

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T

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T

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D

L

L

F

F

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G

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E

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V

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L

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C

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E

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A

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G

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F

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T

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E

L

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G

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S

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A
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A

E

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Y

G

G

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D

Y

Y

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T

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G

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P

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T

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E

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T

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K

A

K

A

A

M

M

K

K

K

G

V

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L

L

E

K

D

D

I

I

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Q

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N

G

G

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V

Y

F

A

A

K

K

S

D

F

F

I

I

L

L

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E

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K

R

A

A

G

G

A

F

P

A

T

R

L

M

M

H

S

A

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E

R

R

R

K

I

N

N

M

A

N

E

D

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S

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L

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I

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E

I

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G

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L

R

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A

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M

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W

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S

A

A

N

K

K

K

L

L

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V

D

D

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A

S

D

K

K

N

N

binding magnesium ion: D191 (= D190), E195 (= E194)
binding (2R)-2,3-dihydroxy-3-methylbutanoic acid: A132 (= A131), P133 (= P132), D191 (= D190), E195 (= E194), E231 (= E230), S252 (= S251), A255 (= A254)

8uppA Campylobacter jejuni ketol-acid reductoisomerase in complex with NADPH and hoe704
64% identity, 97% coverage: 1:327/338 of query aligns to 1:327/327 of 8uppA

query
sites
8uppA

M

I

K

T

V

V

F

Y

Y

Y

D

D

K

K

D

D

A

C

D

D

L

L

S

N

L

L

I

I

K

K

N

S

K

K

N

K

V

V

T

A

I

I

G

G

Y
x
F

G
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G

S
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S

Q
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Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

V

V

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N

V

V

T

T

V

I

G

G

L

L

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R

K

E

G

G

G
x
S

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V

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S

W

A

N

V

K

K

A

A

E

K

Q

N

A

A

G

G

L

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K

E

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V

A

M

E

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N

S

E

E

A

A

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K

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A

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D

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V

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M

M

I

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L

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D
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D

E

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x
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x
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G

A

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T

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H
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G

G

F

F

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N

I

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H

H

Y

Y

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G

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Q

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I

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P

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K

D

G

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D

D

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G

G

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H

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L

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I

A

A

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Q

D

D

K

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G

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S

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A

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D

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L

L

A

A

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S

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Y

A

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S

A

A

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I

E

E

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T

F

F

R

K

E

A

E

E

T

T

E

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T

T

D
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D

L

L

F

F

G

G

E
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E

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Q

A

A

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V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

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K

Q

A

A

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G

F

F

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T

L

L

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E

E

A

A

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Y

A

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P

E

E

M

M

A

A

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Y

F

F

E

E

C

C

L

L

H

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E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I
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I

S
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S

N

N

N

T

A
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A

E

E

Y

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

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P

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K

V

I

I

I

N

T

A

E

E

E

S

T

K

K

A

K

A

A

M

M

K

K

K

G

V

V

L

L

E

K

D

D

I

I

Q

Q

T

N

G

G

E

V

Y

F

A

A

K

K

S

D

F

F

I

I

L

L

E

E

N

R

K

R

A

A

G

G

A

F

P

A

T

R

L

M

M

H

S

A

R

E

R

R

R

K

I

N

N

M

A

N

E

D

H

S

Q

L

I

I

E

E

I

K

V

T

G

G

E

R

K

N

L

L

R

R

A

A

M

M

P

P

W

W

I

I

binding magnesium ion: D190 (= D190), E194 (= E194)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F24 (≠ Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), R47 (= R47), S50 (≠ G50), L79 (= L79), P81 (= P81), D82 (= D82), I84 (≠ N84), Q85 (≠ I85), I88 (≠ V88), H107 (= H107), P132 (= P132), I250 (= I250), S251 (= S251)
binding (2R)-(dimethylphosphoryl)(hydroxy)acetic acid: A131 (= A131), P132 (= P132), D190 (= D190), E194 (= E194), E230 (= E230), I250 (= I250), S251 (= S251), A254 (= A254)

8sxdA Campylobacter jejuni keto-acid reductoisomerase in complex with intermediate and NADP+
64% identity, 97% coverage: 1:327/338 of query aligns to 1:327/327 of 8sxdA

query
sites
8sxdA

M

I

K

T

V

V

F

Y

Y

Y

D

D

K

K

D

D

A

C

D

D

L

L

S

N

L

L

I

I

K

K

N

S

K

K

N

K

V

V

T

A

I

I

G
|
G

Y
x
F

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

V

V

K

N

V

V

T

T

V

I

G

G

L

L

R

R

K

E

G

G

G
x
S

A

V

S
|
S

W

A

N

V

K

K

A

A

E

K

Q

N

A

A

G

G

L

F

K

E

V

V

A

M

E

S

V

V

N

S

E

E

A

A

V

S

K

K

D

I

A

A

D

D

V

V

I

I

M

M

I

I

L
|
L

L
x
A

P
|
P

D
|
D

E

E

N

I

I

Q

G

A

Q

D

V

I

Y

F

A

N

E

V

N

E

V

I

A

K

P

P

F

N

A

L

K

S

Q

E

G

G

A

K

T

A

V

I

A

A

F

F

A

A

H

H

G

G

F

F

N

N

I

I

H

H

Y

Y

G

G

Q

Q

V

I

V

V

P

V

R

P

A

K

D

G

L

V

D

D

I

V

I

I

M

M

I

I

A

A

P
|
P

K

K

A

A

P

P

G

G

H

H

T

T

V

V

R

R

S

N

T

E

Y

F

S

T

Q

L

G

G

V

T

P

P

H

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

K

E

S

S

G

K

S

N

A

A

R

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

T

S

A

A

N

I

G

G

R

R

A

T

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

I

I

K

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

N

T

A

A

E

E

Y

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

V

I

I

I

N

T

A

E

E

E

S

T

K

K

A

K

A

A

M

M

K

K

K

G

V

V

L

L

E

K

D

D

I

I

Q

Q

T

N

G

G

E

V

Y

F

A

A

K

K

S

D

F

F

I

I

L

L

E

E

N

R

K

R

A

A

G

G

A

F

P

A

T

R

L

M

M

H

S

A

R

E

R

R

R

K

I

N

N

M

A

N

E

D

H

S

Q

L

I

I

E

E

I

K

V

T

G

G

E

R

K

N

L

L

R

R

A

A

M

M

P

P

W

W

I

I

binding magnesium ion: D190 (= D190), E194 (= E194)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G23 (= G23), F24 (≠ Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), S50 (≠ G50), S52 (= S52), L79 (= L79), A80 (≠ L80), P81 (= P81), D82 (= D82), P129 (= P129)
binding 3-hydroxy-3-methyl-2-oxobutanoic acid: D190 (= D190), E194 (= E194)

7rduA Crystal structure of campylobacter jejuni keto said reductoisomerase in complex with magnesium and oxidixized and reduced NADPH
64% identity, 97% coverage: 1:327/338 of query aligns to 2:328/329 of 7rduA

query
sites
7rduA

M

I

K

T

V

V

F

Y

Y

Y

D

D

K

K

D

D

A

C

D

D

L

L

S

N

L

L

I

I

K

K

N

S

K

K

N

K

V

V

T

A

I

I

G
|
G

Y
x
F

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

V

V

K

N

V

V

T

T

V

I

G

G

L

L

R
|
R

K

E

G

G

G
x
S

A

V

S
|
S

W

A

N

V

K

K

A

A

E

K

Q

N

A

A

G

G

L

F

K

E

V

V

A

M

E

S

V

V

N

S

E

E

A

A

V

S

K

K

D

I

A

A

D

D

V

V

I

I

M

M

I

I

L
|
L

L
x
A

P
|
P

D
|
D

E

E

N

I

I

Q

G

A

Q

D

V
x
I

Y

F

A

N

E

V

N

E

V

I

A

K

P

P

F

N

A

L

K

S

Q

E

G

G

A

K

T

A

V

I

A

A

F

F

A
|
A

H
|
H

G

G

F

F

N

N

I

I

H

H

Y

Y

G

G

Q

Q

V

I

V

V

P

V

R

P

A

K

D

G

L

V

D

D

I

V

I

I

M

M

I

I

A

A

P
|
P

K
|
K

A
|
A

P

P

G

G

H

H

T

T

V

V

R

R

S

N

T

E

Y

F

S

T

Q

L

G

G

V

T

P

P

H

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

K

E

S

S

G

K

S

N

A

A

R

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

T

S

A

A

N

I

G

G

R

R

A

T

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

A

E

E

T

T

E

E

T

T

D

D

L

L

F

F

G

G

E

E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

I

I

K

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

N

T

A

A

E

E

Y

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

V

I

I

I

N

T

A

E

E

E

S

T

K

K

A

K

A

A

M

M

K

K

K

G

V

V

L

L

E

K

D

D

I

I

Q

Q

T

N

G

G

E

V

Y

F

A

A

K

K

S

D

F

F

I

I

L

L

E

E

N

R

K

R

A

A

G

G

A

F

P

A

T

R

L

M

M

H

S

A

R

E

R

R

R

K

I

N

N

M

A

N

E

D

H

S

Q

L

I

I

E

E

I

K

V

T

G

G

E

R

K

N

L

L

R

R

A

A

M

M

P

P

W

W

I

I

binding nadp nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), G26 (= G25), S27 (= S26), Q28 (= Q27), R48 (= R47), S51 (≠ G50), S53 (= S52), A81 (≠ L80), P82 (= P81), D83 (= D82), I89 (≠ V88), A107 (= A106), H108 (= H107), P130 (= P129), K131 (= K130), A132 (= A131)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), F25 (≠ Y24), G26 (= G25), S27 (= S26), Q28 (= Q27), S51 (≠ G50), S53 (= S52), L80 (= L79), P82 (= P81), D83 (= D82), I89 (≠ V88), A107 (= A106), H108 (= H107)

8swmA Crystal structure of campylobacter jejuni ketol-acid reductoisomerase in complex with 2-acetolactate
64% identity, 96% coverage: 4:326/338 of query aligns to 1:323/323 of 8swmA

query
sites
8swmA

F

Y

Y

Y

D

D

K

K

D

D

A

C

D

D

L

L

S

N

L

L

I

I

K

K

N

S

K

K

N

K

V

V

T

A

I

I

G

G

Y

F

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

V

V

K

N

V

V

T

T

V

I

G

G

L

L

R

R

K

E

G

G

G

S

A

V

S

S

W

A

N

V

K

K

A

A

E

K

Q

N

A

A

G

G

L

F

K

E

V

V

A

M

E

S

V

V

N

S

E

E

A

A

V

S

K

K

D

I

A

A

D

D

V

V

I

I

M

M

I

I

L

L

L

A

P

P

D

D

E

E

N

I

I

Q

G

A

Q

D

V

I

Y

F

A

N

E

V

N

E

V

I

A

K

P

P

F

N

A

L

K

S

Q

E

G

G

A

K

T

A

V

I

A

A

F

F

A

A

H

H

G

G

F

F

N

N

I

I

H

H

Y

Y

G

G

Q

Q

V

I

V

V

P

V

R

P

A

K

D

G

L

V

D

D

I

V

I

I

M

M

I

I

A

A

P

P

K

K

A

A

P

P

G

G

H

H

T

T

V

V

R

R

S

N

T

E

Y

F

S

T

Q

L

G

G

V

T

P

P

H

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

K

E

S

S

G

K

S

N

A

A

R

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

T

S

A

A

N

I

G

G

R

R

A

T

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

I

I

K

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

N

T

A

A

E

E

Y

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

V

I

I

I

N

T

A

E

E

E

S

T

K

K

A

K

A

A

M

M

K

K

K

G

V

V

L

L

E

K

D

D

I

I

Q

Q

T

N

G

G

E

V

Y

F

A

A

K

K

S

D

F

F

I

I

L

L

E

E

N

R

K

R

A

A

G

G

A

F

P

A

T

R

L

M

M

H

S

A

R

E

R

R

R

K

I

N

N

M

A

N

E

D

H

S

Q

L

I

I

E

E

I

K

V

T

G

G

E

R

K

N

L

L

R

R

A

A

M

M

P

P

W

W

binding magnesium ion: D187 (= D190), E191 (= E194)
binding (2S)-2-hydroxy-2-methyl-3-oxobutanoic acid: D187 (= D190), E191 (= E194)

C8WR67 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / BCRC 14685 / JCM 5260 / KCTC 1825 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-IA) (Bacillus acidocaldarius) (see paper)
61% identity, 99% coverage: 2:334/338 of query aligns to 3:334/344 of C8WR67

query
sites
C8WR67

K

K

V

I

F

Y

Y

Y

D

D

K

A

D

D

A

I

D

S

L

I

S

Q

L

P

I

L

K

A

N

D

K

K

N

R

V

I

T

A

I

V

I

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

Q

N

N

L

L

K

R

D

D

S

S

G

G

V

F

K

D

V

V

T

V

V

I

G

G

L

L

R
|
R

K

P

G

G

G

-

A

S

S
|
S

W

W

N

A

K

K

A

A

E

E

Q

A

A

D

G

G

L

F

K

R

V

V

A

M

E

A

V

V

N

G

E

E

A

A

V

V

K

E

D

E

A

S

D

D

V

V

I

I

M

M

I

I

L

L

P

P

D
|
D

E
|
E

N
x
R

I
x
Q

G

P

Q

A

V

V

Y

Y

A

E

E

R

N

E

V

I

A

R

P

P

F

Y

A

L

K

T

Q

A

G

G

A

K

T

A

V

L

A

A

F

F

A

A

H

H

G

G

F

F

N

N

I

I

H

H

Y

F

G

S

Q

Q

V

I

V

Q

P

P

R

P

A

K

D

D

L

V

D

D

I

V

I

F

M

M

I

V

A

A

P

P

K

K

A

G

P

P

G
|
G

H

H

T

L

V

V

R

R

S

R

T

V

Y

Y

S

E

Q

A

G

G

V

V

P

P

H

A

L

L

I

I

A

A

V

V

H

H

Q

Q

D

D

K

A

S

S

G

G

S

Q

A

A

R

K

D

D

L

L

A

A

L

L

S

A

Y

Y

A

A

T

R

A

G

N

I

G

G

G

A

G

G

R

R

A

A

G

G

I

I

I

L

E

T

T

T

N

T

F

F

R

R

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

I

I

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

I

A

A

Y

Y

F

F

E
|
E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

E

G

G

I

L

A

E

N

Y

M

M

N

R

Y

Y

S

S

I

I

S

S

N

D

N

T

A

A

E

Q

Y

W

G

G

E

D

Y

F

V

T

T

S

G

G

P

P

R

R

V

I

I

I

N

N

A

E

E

E

S

T

K

K

A

K

A

E

M

M

K

R

V

I

L

L

E

A

D

D

I

I

Q

Q

T

S

G

G

E

A

Y

F

A

A

K

K

S

S

F

W

I

I

L

L

E

E

N

N

K

Q

A

A

G

N

A

R

P

P

T

M

L

F

M

N

S

A

R

I

R

N

R

R

I

R

N

E

A

L

E

E

H

H

Q

P

I

I

E

E

I

V

V

V

G

G

E

R

K

K

L

L

R

R

A

S

M

M

P

P

W

F

I

I

K

K

A

A

N

K

K

R

L

P

V

G

D

D

YGSQ 25:28 (= YGSQ 24:27) binding NADP(+)
R48 (= R47) binding NADP(+); mutation to P: Inversion of the cofactor specificity from NADPH to NADH.
S52 (= S52) binding NADP(+); mutation to D: Inversion of the cofactor specificity from NADPH to NADH.
DERQ 82:85 (≠ DENI 82:85) binding NADP(+)
G133 (= G133) binding NADP(+)
D190 (= D190) binding Mg(2+)
E194 (= E194) binding Mg(2+)
E226 (= E226) binding Mg(2+)

8cy8A Apo form cryo-em structure of campylobacter jejune ketol-acid reductoisommerase crosslinked by glutaraldehyde
62% identity, 97% coverage: 1:327/338 of query aligns to 1:311/312 of 8cy8A

query
sites
8cy8A

M

I

K

T

V

V

F

Y

Y

Y

D

D

K

K

D

D

A

C

D

D

L

L

S

N

L

L

I

I

K

K

N

S

K

K

N

K

V

V

T

A

I

I

G

G

Y

F

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

V

V

K

N

V

V

T

T

V

I

G

G

L

L

R

R

K

-

G

-

A

-

S

-

W

-

N

-

K

-

A

-

E

-

Q

N

A

A

G

G

L

F

K

E

V

V

A

M

E

S

V

V

N

S

E

E

A

A

V

S

K

K

D

I

A

A

D

D

V

V

I

I

M

M

I

I

L

L

L

A

P

P

D

D

E

E

N

I

I

Q

G

A

Q

D

V

I

Y

F

A

N

E

V

N

E

V

I

A

K

P

P

F

N

A

L

K

S

Q

E

G

G

A

K

T

A

V

I

A

A

F

F

A

A

H

H

G

G

F

F

N

N

I

I

H

H

Y

Y

G

G

Q

Q

V

I

V

V

P

V

R

P

A

K

D

G

L

V

D

D

I

V

I

I

M

M

I

I

A

A

P

P

K

K

A

A

P

P

G

G

H

H

T

T

V

V

R

R

S

N

T

E

Y

-

S

-

Q

-

G

-

V

T

P

P

H

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

K

E

S

S

G

K

S

N

A

A

R

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

T

S

A

A

N

I

G

G

R

R

A

T

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L
|
L

C

C

G

G

T

L

V

S

E

A

L

L

I

I

K

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

N

T

A

A

E

E

Y

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

V

I

I

I

N

T

A

E

E

E

S

T

K

K

A

K

A

A

M

M

K

K

K

G

V

V

L

L

E

K

D

D

I

I

Q

Q

T

N

G

G

E

V

Y

F

A

A

K

K

S

D

F

F

I

I

L

L

E

E

N

R

K

R

A

A

G

G

A

F

P

A

T

R

L

M

M

H

S

A

R

E

R

R

R

K

I

N

N

M

A

N

E

D

H

S

Q

L

I

I

E

E

I

K

V

T

G

G

E

R

K

N

L

L

R

R

A

A

M

M

P

P

W

W

I

I

binding pentanedial: D174 (= D190), E178 (= E194), L182 (= L198)

4tskA Ketol-acid reductoisomerase from alicyclobacillus acidocaldarius (see paper)
61% identity, 99% coverage: 2:334/338 of query aligns to 2:333/333 of 4tskA

query
sites
4tskA

K

K

V

I

F

Y

Y

Y

D

D

K

A

D

D

A

I

D

S

L

I

S

Q

L

P

I

L

K

A

N

D

K

K

N

R

V

I

T

A

I

V

I

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

Q

N

N

L

L

K

R

D

D

S

S

G

G

V

F

K

D

V

V

T

V

V

I

G

G

L
|
L

R
|
R

K

P

G

G

G

-

A

S

S
|
S

W

W

N

A

K

K

A

A

E

E

Q

A

A

D

G

G

L

F

K

R

V

V

A

M

E

A

V

V

N

G

E

E

A

A

V

V

K

E

D

E

A

S

D

D

V

V

I

I

M

M

I

I

L
|
L

P
|
P

D
|
D

E

E

N

R

I

Q

G

P

Q

A

V

V

Y

Y

A

E

E

R

N

E

V

I

A

R

P

P

F

Y

A

L

K

T

Q

A

G

G

A

K

T

A

V

L

A

A

F

F

A

A

H
|
H

G

G

F

F

N

N

I

I

H

H

Y

F

G

S

Q

Q

V

I

V

Q

P

P

R

P

A

K

D

D

L

V

D

D

I

V

I

F

M

M

I

V

A

A

P

P

K
|
K

A

G

P
|
P

G

G

H

H

T

L

V

V

R

R

S

R

T

V

Y

Y

S

E

Q

A

G

G

V

V

P

P

H

A

L

L

I

I

A

A

V

V

H

H

Q

Q

D

D

K

A

S

S

G

G

S

Q

A

A

R

K

D

D

L

L

A

A

L

L

S

A

Y

Y

A

A

T

R

A

G

N

I

G

G

G

A

G

G

R

R

A

A

G

G

I

I

I

L

E

T

T

T

N

T

F

F

R

R

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

I

I

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

I

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

E

G

G

I

L

A

E

N

Y

M

M

N
x
R

Y
|
Y

S

S

I
|
I

S

S

N
x
D

N

T

A

A

E
x
Q

Y

W

G

G

E

D

Y

F

V

T

T

S

G

G

P

P

R

R

V

I

I

I

N

N

A

E

E

E

S

T

K

K

A

K

A

E

M

M

K

R

V

I

L

L

E

A

D

D

I

I

Q

Q

T

S

G

G

E

A

Y

F

A

A

K

K

S

S

F

W

I

I

L

L

E

E

N

N

K

Q

A

A

G

N

A

R

P

P

T

M

L

F

M

N

S

A

R

I

R

N

R

R

I

R

N

E

A

L

E

E

H

H

Q

P

I

I

E

E

I

V

V

V

G

G

E

R

K

K

L

L

R

R

A

S

M

M

P

P

W

F

I

I

K

K

A

A

N

K

K

R

L

P

V

G

D

D

active site: K129 (= K130), D189 (= D190), E193 (= E194)
binding magnesium ion: D189 (= D190), D189 (= D190), E193 (= E194), E193 (= E194), R246 (≠ N247), Y247 (= Y248), I249 (= I250), D251 (≠ N252), Q254 (≠ E255)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), L46 (= L46), R47 (= R47), S51 (= S52), L78 (= L79), L79 (= L80), P80 (= P81), D81 (= D82), H106 (= H107), P131 (= P132)

D0WGK0 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Slackia exigua (strain ATCC 700122 / DSM 15923 / CIP 105133 / JCM 11022 / KCTC 5966 / S-7) (see paper)
58% identity, 97% coverage: 1:328/338 of query aligns to 12:339/342 of D0WGK0

query
sites
D0WGK0

M

V

K

T

V

I

F

L

Y

Y

D

E

K

Q

D

D

A

V

D

D

L

P

S

K

L

V

I

I

K

Q

N

G

K

L

N

K

V

V

T

G

I

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

K

M

D

D

S

S

G

G

V

V

K

D

V

V

T

R

V

V

G

G

L

L

R
|
R

K

E

G

G

G
x
S

A

S

S
|
S

W

W

N

K

K

T

A

A

E

E

Q

E

A

A

G

G

L

L

K

K

V

V

A

T

E

D

V

M

N

D

E

T

A

A

V

A

K

E

D

E

A

A

D

D

V

V

I

I

M

M

I

V

L

L

L

V

P

P

D
|
D

E
|
E

N
x
I

I
x
Q

G

P

Q

K

V

V

Y

Y

A

Q

E

E

N

H

V

I

A

A

P

A

F

H

A

L

K

K

Q

A

G

G

A

N

T

T

V

L

A

A

F

F

A

A

H

H

G

G

F

F

N

N

I

I

H

H

Y

Y

G

G

Q

Y

V

I

V

V

P

P

R

P

A

E

D

D

L

V

D

N

I

V

I

I

M

M

I

C

A

A

P

P

K

K

A

G

P

P

G
|
G

H

H

T

I

V

V

R

R

S

R

T

Q

Y

F

S

T

Q

E

G

G

G

S

G

G

V

V

P

P

H

D

L

L

I

A

A

C

V

V

H

Q

Q

Q

D

D

K

A

S

T

G

G

S

N

A

A

R

W

D

D

L

I

A

V

L

L

S

S

Y

Y

A

C

T

W

A

G

N

V

G

G

G

A

R

R

A

S

G

G

I

I

E

K

T

A

N

T

F

F

R

A

E

E

T

T

E

E

T

E

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

V

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

A

P

P

P

E

E

M

L

A

A

Y

Y

F

F

E

E

C

C

L

Y

H

H

E

E

L

M

K

K

L

M

I

I

V

V

D

D

L

L

I

M

Y

Y

E

E

G

S

G

G

I

I

A

H

N

F

M

M

N

N

Y

Y

S

S

I

I

S
|
S

N

N

N

T

A

A

E

E

Y

Y

G

G

E

E

Y

Y

V

Y

T

A

G

G

P

P

R

K

V

V

I

I

N

N

A

E

E

Q

S

S

K

R

A

E

A

A

M

M

K

K

K

E

V

I

L

L

E

K

D

R

I

I

Q

Q

T

D

G

G

E

S

Y

F

A

A

K

Q

S

E

F

F

I

V

L

D

E

D

N

C

K

N

A

N

G

G

A

H

P

K

T

R

L

L

M

L

S

E

R

Q

R

R

R

E

I

A

N

I

A

N

E

T

H

H

Q

P

I

I

E

E

I

T

V

T

G

G

E

A

K

Q

L

I

R

R

A

S

M

M

M

F

P

S

W

W

I

I

K

K

YGSQ 35:38 (= YGSQ 24:27) binding NADP(+)
R58 (= R47) binding NADP(+)
S61 (≠ G50) binding NADP(+); mutation to D: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold decrease of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 2-fold increase of the catalytic efficiency for NADH; when associated with D-63.
S63 (= S52) binding NADP(+); mutation to D: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold decrease of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 2-fold increase of the catalytic efficiency for NADH; when associated with D-61.
DEIQ 93:96 (≠ DENI 82:85) binding NADP(+)
G144 (= G133) binding NADP(+)
D201 (= D190) binding Mg(2+)
E205 (= E194) binding Mg(2+)
S262 (= S251) binding substrate

4kqxB Mutant slackia exigua kari ddv in complex with NAD and an inhibitor (see paper)
57% identity, 98% coverage: 1:332/338 of query aligns to 3:334/335 of 4kqxB

query
sites
4kqxB

M

V

K

T

V

I

F

L

Y

Y

D

E

K

Q

D

D

A

V

D

D

L

P

S

K

L

V

I

I

K

Q

N

G

K

L

N

K

V

V

T

G

I

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G
|
G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

K

M

D

D

S

S

G

G

V

V

K

D

V

V

T

R

V

V

G

G

L

L

R
|
R

K

E

G

G

G

D

A

S

S

D

W

W

N

K

K

T

A

A

E

E

Q

E

A

A

G

G

L

L

K

K

V

V

A

T

E

D

V

M

N

D

E

T

A

A

V
x
A

K
x
E

D

E

A
|
A

D

D

V

V

I

I

M

M

I

V

L
|
L

L
x
V

P
|
P

D
|
D

E

E

N

V

I

Q

G

P

Q

K

V
|
V

Y

Y

A

Q

E

E

N

H

V

I

A

A

P

A

F

H

A

L

K

K

Q

A

G

G

A
x
N

T

T

V

L

A

A

F

F

A

A

H
|
H

G

G

F

F

N

N

I

I

H

H

Y

Y

G

G

Q

Y

V

I

V

V

P

P

R

P

A

E

D

D

L

V

D

N

I

V

I

I

M

M

I

C

A

A

P

P

K
|
K

A

G

P
|
P

G

G

H

H

T

I

V

V

R

R

S

R

T

Q

Y

F

S

T

Q

E

G

G

G

S

G

G

V

V

P

P

H

D

L

L

I

A

A

C

V

V

H

Q

Q

Q

D

D

K

A

S

T

G

G

S

N

A

A

R

W

D

D

L

I

A

V

L

L

S

S

Y

Y

A

C

T

W

A

G

N

V

G

G

G

A

R

R

A

S

G

G

I

I

E

K

T

A

N

T

F

F

R

A

E

E

T

T

E

E

T

E

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

V

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

A

P

P

P

E

E

M

L

A

A

Y

Y

F

F

E

E

C

C

L

Y

H

H

E
|
E

L

M

K

K

L

M

I

I

V

V

D

D

L

L

I

M

Y

Y

E

E

G

S

G

G

I

I

A

H

N

F

M

M

N
|
N

Y

Y

S
|
S

I
|
I

S
|
S

N
|
N

N

T

A
|
A

E
|
E

Y

Y

G

G

E

E

Y

Y

V

Y

T

A

G

G

P

P

R

K

V

V

I

I

N

N

A

E

E

Q

S

S

K

R

A

E

A

A

M

M

K

K

K

E

V

I

L

L

E

K

D

R

I

I

Q

Q

T

D

G

G

E

S

Y

F

A

A

K

Q

S

E

F

F

I

V

L

D

E

D

N

C

K

N

A

N

G

G

A

H

P

K

T

R

L

L

M

L

S

E

R

Q

R

R

R

E

I

A

N

I

A

N

E

T

H

H

Q

P

I

I

E

E

I

T

V

T

G

G

E

A

K

Q

L

I

R

R

A

S

M

M

M

F

P

S

W

W

I

I

K

K

A

K

N

E

K
x
D

L
|
L

active site: K132 (= K130), D192 (= D190), E196 (= E194)
binding n-hydroxy-n-isopropyloxamic acid: P134 (= P132), D192 (= D190), E196 (= E194), E232 (= E230), I252 (= I250), S253 (= S251), A256 (= A254)
binding histidine: D333 (≠ K331), L334 (= L332)
binding magnesium ion: G27 (= G25), Q29 (= Q27), G30 (= G28), A72 (≠ V70), E73 (≠ K71), A75 (= A73), L81 (= L79), N103 (≠ A101), D192 (= D190), E196 (= E194), N249 (= N247), S251 (= S249), I252 (= I250), I252 (= I250), S253 (= S251), N254 (= N252), E257 (= E255)
binding nicotinamide-adenine-dinucleotide: Y26 (= Y24), G27 (= G25), S28 (= S26), Q29 (= Q27), R49 (= R47), L81 (= L79), V82 (≠ L80), P83 (= P81), D84 (= D82), V90 (= V88), H109 (= H107), P134 (= P132), S251 (= S249), I252 (= I250), S253 (= S251)

Sites not aligning to the query:

binding histidine: 335

4kqwA The structure of the slackia exigua kari in complex with NADP (see paper)
58% identity, 96% coverage: 3:327/338 of query aligns to 2:326/326 of 4kqwA

query
sites
4kqwA

V

I

F

L

Y

Y

D

E

K

Q

D

D

A

V

D

D

L

P

S

K

L

V

I

I

K

Q

N

G

K

L

N

K

V

V

T

G

I

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

K

M

D

D

S

S

G

G

V

V

K

D

V

V

T

R

V

V

G

G

L

L

R
|
R

K

E

G

G

G
x
S

A

S

S
|
S

W

W

N

K

K

T

A

A

E

E

Q

E

A

A

G

G

L

L

K

K

V

V

A

T

E

D

V

M

N

D

E

T

A

A

V

A

K

E

D

E

A

A

D

D

V

V

I

I

M

M

I

V

L
|
L

L
x
V

P
|
P

D
|
D

E

E

N
x
I

I
x
Q

G

P

Q

K

V

V

Y

Y

A

Q

E

E

N

H

V

I

A

A

P

A

F

H

A

L

K

K

Q

A

G

G

A

N

T

T

V

L

A

A

F

F

A

A

H
|
H

G

G

F

F

N

N

I

I

H

H

Y

Y

G

G

Q

Y

V

I

V

V

P

P

R

P

A

E

D

D

L

V

D

N

I

V

I

I

M

M

I

C

A

A

P

P

K
|
K

A

G

P
|
P

G

G

H

H

T

I

V

V

R

R

S

R

T

Q

Y

F

S

T

Q

E

G

G

G

S

G

G

V

V

P

P

H

D

L

L

I

A

A

C

V

V

H

Q

Q

Q

D

D

K

A

S

T

G

G

S

N

A

A

R

W

D

D

L

I

A

V

L

L

S

S

Y

Y

A

C

T

W

A

G

N

V

G

G

G

A

R

R

A

S

G

G

I

I

E

K

T

A

N

T

F

F

R

A

E

E

T

T

E

E

T

E

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

V

E

E

L

L

I

V

K

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

A

P

P

P

E

E

M

L

A

A

Y

Y

F

F

E

E

C

C

L

Y

H

H

E

E

L

M

K

K

L

M

I

I

V

V

D

D

L

L

I

M

Y

Y

E

E

G

S

G

G

I

I

A

H

N

F

M

M

N

N

Y

Y

S

S

I
|
I

S
|
S

N

N

N

T

A

A

E

E

Y

Y

G

G

E

E

Y

Y

V

Y

T

A

G

G

P

P

R

K

V

V

I

I

N

N

A

E

E

Q

S

S

K

R

A

E

A

A

M

M

K

K

K

E

V

I

L

L

E

K

D

R

I

I

Q

Q

T

D

G

G

E

S

Y

F

A

A

K

Q

S

E

F

F

I

V

L

D

E

D

N

C

K

N

A

N

G

G

A

H

P

K

T

R

L

L

M

L

S

E

R

Q

R

R

R

E

I

A

N

I

A

N

E

T

H

H

Q

P

I

I

E

E

I

T

V

T

G

G

E

A

K

Q

L

I

R

R

A

S

M

M

M

F

P

S

W

W

I

I

active site: K129 (= K130), D189 (= D190), E193 (= E194)
binding magnesium ion: D189 (= D190), E193 (= E194)
binding nadp nicotinamide-adenine-dinucleotide phosphate: Y23 (= Y24), G24 (= G25), S25 (= S26), Q26 (= Q27), R46 (= R47), S49 (≠ G50), S51 (= S52), L78 (= L79), V79 (≠ L80), P80 (= P81), D81 (= D82), I83 (≠ N84), Q84 (≠ I85), H106 (= H107), P131 (= P132), I249 (= I250), S250 (= S251)

7q03A Ketol-acid reductoisomerase from methanothermococcus thermolithotrophicus in the close state with NADP and mg2+ (see paper)
57% identity, 96% coverage: 1:323/338 of query aligns to 1:323/328 of 7q03A

query
sites
7q03A

M

M

K

K

V

V

F

F

Y

Y

D

D

K

S

D

D

A

T

D

T

L

F

S

D

L

A

I

V

K

K

N

D

K

K

N

T

V

I

T

A

I

V

I

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|