SitesBLAST
Comparing HSERO_RS09025 FitnessBrowser__HerbieS:HSERO_RS09025 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3wx9A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, gla, 4ad, 2og, glu and kya
30% identity, 69% coverage: 148:490/500 of query aligns to 38:387/404 of 3wx9A
- binding 4-(2-aminophenyl)-2,4-dioxobutanoic acid: G40 (= G150), L41 (= L151)
- binding 2-oxoglutaric acid: D213 (= D318), P214 (≠ I319), Y215 (= Y320), G216 (≠ C321), E217 (≠ D322), G241 (= G348), T242 (≠ G349), I246 (≠ T353)
- binding (2E)-pent-2-enedioic acid: G40 (= G150), Y130 (≠ W240), N184 (= N289), R376 (= R479)
- binding glutamic acid: L131 (≠ F241), V360 (≠ A463), A364 (≠ L467), R369 (≠ Q472)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G214), S105 (≠ V215), Q106 (≠ T216), Y130 (≠ W240), N184 (= N289), D212 (= D317), P214 (≠ I319), Y215 (= Y320), T242 (≠ G349), S244 (= S351), K245 (= K352), R252 (= R359)
Sites not aligning to the query:
3av7A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, kyn as substrates and kya as products
30% identity, 69% coverage: 148:490/500 of query aligns to 38:387/404 of 3av7A
- binding 4-hydroxyquinoline-2-carboxylic acid: L131 (≠ F241), Q135 (≠ G245), A364 (≠ L467), R369 (≠ Q472)
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: G40 (= G150), Y130 (≠ W240), L131 (≠ F241), A132 (≠ L242), N184 (= N289), R376 (= R479)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G214), S105 (≠ V215), Q106 (≠ T216), Y130 (≠ W240), V179 (= V286), N184 (= N289), D212 (= D317), P214 (≠ I319), Y215 (= Y320), T242 (≠ G349), S244 (= S351), K245 (= K352), R252 (= R359)
Sites not aligning to the query:
3aowC Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with akg
30% identity, 69% coverage: 148:490/500 of query aligns to 38:387/404 of 3aowC
- binding 2-oxoglutaric acid: Y70 (= Y180), Y130 (≠ W240), L275 (= L382)
- binding pyridoxal-5'-phosphate: G104 (= G214), S105 (≠ V215), Q106 (≠ T216), Y130 (≠ W240), V179 (= V286), N184 (= N289), D212 (= D317), P214 (≠ I319), Y215 (= Y320), T242 (≠ G349), S244 (= S351), K245 (= K352), R252 (= R359)
Sites not aligning to the query:
3aovA Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with plp
30% identity, 69% coverage: 148:490/500 of query aligns to 38:387/404 of 3aovA
- binding pyridoxal-5'-phosphate: G104 (= G214), S105 (≠ V215), Q106 (≠ T216), Y130 (≠ W240), V179 (= V286), N184 (= N289), D212 (= D317), P214 (≠ I319), Y215 (= Y320), T242 (≠ G349), S244 (= S351), K245 (= K352), R252 (= R359)
1wstA Crystal structure of multiple substrate aminotransferase (msat) from thermococcus profundus
29% identity, 69% coverage: 148:490/500 of query aligns to 35:385/403 of 1wstA
- binding pyridoxal-5'-phosphate: G102 (= G214), S103 (≠ V215), Q104 (≠ T216), Y128 (≠ W240), V177 (≠ N284), N182 (= N289), D210 (= D317), P212 (≠ I319), Y213 (= Y320), T240 (≠ G349), S242 (= S351), K243 (= K352), R250 (= R359)
3cbfA Crystal structure of lysn, alpha-aminoadipate aminotransferase, from thermus thermophilus hb27 (see paper)
29% identity, 68% coverage: 149:490/500 of query aligns to 35:375/392 of 3cbfA
- binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: G35 (= G149), G36 (= G150), G95 (= G214), S96 (≠ V215), Q97 (≠ T216), Y121 (≠ W240), N170 (= N289), D198 (= D317), Y201 (= Y320), S231 (≠ G349), S233 (= S351), K234 (= K352), R241 (= R359), R364 (= R479)
Sites not aligning to the query:
2egyA Crystal structure of lysn, alpha-aminoadipate aminotransferase (substrate free form), from thermus thermophilus hb27
29% identity, 68% coverage: 149:490/500 of query aligns to 35:375/392 of 2egyA
- binding pyridoxal-5'-phosphate: G95 (= G214), S96 (≠ V215), Q97 (≠ T216), Y121 (≠ W240), N170 (= N289), D198 (= D317), A200 (≠ I319), Y201 (= Y320), S231 (≠ G349), S233 (= S351), K234 (= K352), R241 (= R359)
2zyjA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-glutamate), from thermus thermophilus hb27 (see paper)
29% identity, 68% coverage: 149:490/500 of query aligns to 39:379/397 of 2zyjA
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: G39 (= G149), G40 (= G150), G99 (= G214), S100 (≠ V215), Q101 (≠ T216), Y125 (≠ W240), N174 (= N289), D202 (= D317), Y205 (= Y320), S235 (≠ G349), S237 (= S351), K238 (= K352), R245 (= R359), R368 (= R479)
Sites not aligning to the query:
Q72LL6 2-aminoadipate transaminase; 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT; EC 2.6.1.39 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
29% identity, 68% coverage: 149:490/500 of query aligns to 39:379/397 of Q72LL6
- G40 (= G150) binding
- Y70 (= Y180) binding
- N174 (= N289) binding ; binding
- R245 (= R359) binding
- R368 (= R479) binding
Sites not aligning to the query:
- 20 S→E: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate. Increases the affinity for leucine and 2-oxoisocaproate.
- 23 R→A: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.; R→Q: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.
2z1yA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-leucine), from thermus thermophilus hb27
29% identity, 68% coverage: 149:490/500 of query aligns to 31:371/389 of 2z1yA
- binding leucine: G32 (= G150), Y117 (≠ W240), R360 (= R479)
- binding pyridoxal-5'-phosphate: G91 (= G214), S92 (≠ V215), Q93 (≠ T216), Y117 (≠ W240), N166 (= N289), D194 (= D317), Y197 (= Y320), S227 (≠ G349), S229 (= S351), K230 (= K352), R237 (= R359)
2zc0A Crystal structure of an archaeal alanine:glyoxylate aminotransferase (see paper)
29% identity, 65% coverage: 158:483/500 of query aligns to 45:383/405 of 2zc0A
- active site: Y132 (≠ W240), D214 (= D317), A216 (≠ I319), S246 (= S351)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G106 (= G214), G107 (≠ V215), T108 (= T216), Y132 (≠ W240), N186 (= N289), D214 (= D317), A216 (≠ I319), Y217 (= Y320), T244 (≠ G349), S246 (= S351), K247 (= K352), R254 (= R359)
1vp4A Crystal structure of a putative aminotransferase (tm1131) from thermotoga maritima msb8 at 1.82 a resolution
28% identity, 69% coverage: 148:490/500 of query aligns to 44:399/420 of 1vp4A
- binding pyridoxal-5'-phosphate: G112 (= G214), S113 (≠ V215), Q114 (≠ T216), Y138 (≠ W240), N194 (= N289), D222 (= D317), P224 (≠ I319), Y225 (= Y320), T252 (≠ G349), S254 (= S351), K255 (= K352), R262 (= R359)
4gebA Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 61% coverage: 181:484/500 of query aligns to 79:404/428 of 4gebA
- binding (5-hydroxy-4-{[(7-hydroxy-6-oxo-2-phenyl-6,7-dihydro-2H-pyrazolo[3,4-b]pyridin-5-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: S117 (≠ V215), Q118 (≠ T216), Y142 (≠ W240), N202 (= N289), D230 (= D317), P232 (≠ I319), Y233 (= Y320), S260 (≠ G349), S262 (= S351), K263 (= K352), R270 (= R359), R399 (= R479)
Sites not aligning to the query:
4ge9A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 61% coverage: 181:484/500 of query aligns to 79:404/428 of 4ge9A
- binding (4-{[(6-benzyl-1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: S117 (≠ V215), Q118 (≠ T216), Y142 (≠ W240), N202 (= N289), D230 (= D317), P232 (≠ I319), Y233 (= Y320), S260 (≠ G349), S262 (= S351), R270 (= R359), L293 (= L382), R399 (= R479)
Sites not aligning to the query:
4ge7A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 61% coverage: 181:484/500 of query aligns to 79:404/428 of 4ge7A
- binding (5-hydroxy-4-{[(1-hydroxy-2-oxo-6-phenoxy-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: S117 (≠ V215), Q118 (≠ T216), Y142 (≠ W240), N202 (= N289), D230 (= D317), P232 (≠ I319), Y233 (= Y320), S260 (≠ G349), S262 (= S351), R270 (= R359), L293 (= L382), R399 (= R479)
Sites not aligning to the query:
4gdyB Kynurenine aminotransferase ii inhibitors
27% identity, 61% coverage: 181:484/500 of query aligns to 79:404/428 of 4gdyB
- binding (5-hydroxy-6-methyl-4-{[(1-oxo-7-phenoxy-1,2-dihydro[1,2,4]triazolo[4,3-a]quinolin-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G116 (= G214), S117 (≠ V215), Q118 (≠ T216), Y142 (≠ W240), N202 (= N289), D230 (= D317), P232 (≠ I319), S260 (≠ G349), S262 (= S351), R270 (= R359), R399 (= R479)
Sites not aligning to the query:
8tn3A Structure of s. Hygroscopicus aminotransferase mppq complexed with pyridoxamine 5'-phosphate (pmp) (see paper)
30% identity, 65% coverage: 148:472/500 of query aligns to 25:360/388 of 8tn3A
4ge4A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 61% coverage: 181:484/500 of query aligns to 73:398/422 of 4ge4A
- binding (5-hydroxy-4-{[(1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: G110 (= G214), S111 (≠ V215), Q112 (≠ T216), Y136 (≠ W240), N196 (= N289), D224 (= D317), P226 (≠ I319), Y227 (= Y320), S254 (≠ G349), S256 (= S351), K257 (= K352), R264 (= R359), R393 (= R479)
Sites not aligning to the query:
7zn5B Cryo-em structure of holo-pdxr from bacillus clausii bound to its target DNA in the closed conformation, c2 symmetry. (see paper)
25% identity, 86% coverage: 40:469/500 of query aligns to 7:400/435 of 7zn5B
Sites not aligning to the query:
2vgzA Crystal structure of human kynurenine aminotransferase ii (see paper)
27% identity, 61% coverage: 181:484/500 of query aligns to 68:393/414 of 2vgzA
Query Sequence
>HSERO_RS09025 FitnessBrowser__HerbieS:HSERO_RS09025
MTNTASQHPTRQNEPHPRGTGPDSGQPGWPLLLRESGDSLVEQIVRGIRARIDDKLLRSG
MRMPSIRQFADTNQISRFTVVEAYDRLVAQGYLESRRGSGFYVKERAPLGGSYGAAQAEL
DTEPGRGLDVVWLVRNMFRQMPPAKMPGGGLLPPDWLDGELIANALRAVSRDNPQLLLGY
GTPQGFLPLRQQLQLKLAELEIAAAPEQIVLTTGVTQGLDTVARHFLRPGDVIFVDDPAW
FLMFGSFAALGAKVVGIPRLGDGPDIARLRELAAVHKPKLYVINSVLHNPTSTSLSAAKA
FQVLRIAEEHDFVIVEDDIYCDMHPGSAVQPATRIAALDQLQRVIYLGGFSKTLSANLRV
GYIATSAELARSLADRKLLSTLTTGEIGERVAYKVLSEGHYRKHAERVRARLNGARDKVM
RQLERLGMDVVEAPPCGMFLWVDTGRDTNVMTEAAMQEGFLLAPGSLFSPAQLPSTYMRI
NVASMADPGIWRFLAQAMER
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory