SitesBLAST

YI 11:12 (= YI 14:15) binding
DNLCNS 31:36 (= DNLCNS 34:39) binding
DI 58:59 (= DI 61:62) binding
FAGLK 80:84 (= FAGLK 83:87) binding
N99 (= N102) binding
S124 (= S127) binding ; mutation to A: No major structural changes. Catalytic efficiency is very low and affinity binding is 21% of the wild-type enzyme.; mutation to T: No major structural changes. Catalytic efficiency is about 30% of that of the wild-type enzyme, and affinity binding is similar to that of the native enzyme.
Y149 (= Y151) active site, Proton acceptor; binding ; mutation to F: No major structural changes. Catalytic efficiency is very low and affinity binding is 12% of the wild-type enzyme.
K153 (= K155) binding ; mutation to A: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.; mutation to M: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.
F178 (= F180) binding
Y299 (≠ C301) mutation to C: Loss of epimerase activity with UDP-Gal by almost 5-fold, but it results in a gain of epimerase activity with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by 230-fold with minimal changes in its three-dimensional structure.

1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
59% identity, 95% coverage: 6:332/343 of query aligns to 3:330/338 of 1udaA

query
sites
1udaA

I

V

L

L

V

V

T

T

G
|
G

G

G

A
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

I

L

H

Q

A

N

G

G

Y

H

A

D

V

V

V

I

L

L

D
|
D

N
|
N

L
|
L

C
|
C

N
|
N

S
|
S

R

K

A

R

S

S

V

V

I

L

D

P

R

V

I

I

A

E

Q

R

I

L

S

G

G

G

Q

K

R

H

P

P

H

T

F

F

V

V

Q

E

G
|
G

D
|
D

I
|
I

R
|
R

D

N

R

E

A

A

V

L

L

M

D

T

G

E

I

I

F

L

K

H

S

D

H

H

R

A

I

I

E

D

A

T

V

V

I

I

H

H

F
|
F

A
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A

G
|
G

L

L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

D

D

N
|
N

N

N

V

V

H

N

G

G

S

T

N

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

A

A

H

A

G

N

V

V

K

K

N

N

I

F

V

I

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

P

N

A

P

S

K

V

I

P

P

I

Y

T

V

E

E

E

S

F

F

P

P

L

T

-

G

S

T

A

P

T

Q

N

S

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

G

T

D

D

L

L

H

Q

V

K

A

A

D

Q

P

P

S

D

W

W

R

S

I

I

A

A

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

I
x
M

G

G

E

E

D

D

P

P

S

Q

G

G

I

I

P

P

N
|
N

L
|
L

L

M

P

P

F
x
Y

I

I

A

A

Q

Q

V

V

A

A

D

V

G

G

R

R

A

D

A

S

L
|
L

S
x
A

V
x
I

Y
x
F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

L

D

G

G

H

H

L

V

K

V

T

A

L

M

R

E

K

K

L

L

E

A

Q

N

G

K

P

P

G

G

V

V

Y

H

T

I

Y

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

N

N

S

S

V
|
V

L

L

E

D

M

V

L

V

A

N

A

A

F

F

E

S

Q

K

A

A

C

C

G

G

N

K

K

P

L

V

P

N

Y

Y

Q

H

L

F

V

A

D

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

C

A

C
x
Y

Y

W

A

A

A

D

T

A

E

S

R

K

A

A

E

D

R

R

E

E

L

L

G

N

W

W

R

R

A

V

Q

T

R

R

G

T

I

L

E

D

A

E

M

M

C

A

A

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

A

S

active site: S124 (= S127), A125 (= A128), T126 (= T129), Y149 (= Y151), K153 (= K155), M189 (≠ I191)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), S9 (≠ A12), G10 (= G13), Y11 (= Y14), I12 (= I15), D31 (= D34), N32 (= N35), L33 (= L36), C34 (= C37), N35 (= N38), S36 (= S39), G57 (= G60), D58 (= D61), I59 (= I62), R60 (= R63), F80 (= F83), A81 (= A84), G82 (= G85), K84 (= K87), N99 (= N102), S122 (= S125), S123 (= S126), S124 (= S127), Y149 (= Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: V86 (= V89), S124 (= S127), T126 (= T129), F178 (= F180), N179 (= N181), N198 (= N200), N199 (= N201), L200 (= L202), Y203 (≠ F205), L215 (= L217), A216 (≠ S218), I217 (≠ V219), F218 (≠ Y220), G229 (= G231), R231 (= R233), Y233 (= Y235), V269 (= V271), R292 (= R294), D295 (= D297), Y299 (≠ C301)

1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
59% identity, 95% coverage: 6:332/343 of query aligns to 3:330/338 of 1naiA

query
sites
1naiA

I

V

L

L

V

V

T

T

G
|
G

G

G

A
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

I

L

H

Q

A

N

G

G

Y

H

A

D

V

V

V

I

L

L

D
|
D

N
|
N

L
|
L

C
|
C

N
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N

S
|
S

R

K

A

R

S

S

V

V

I

L

D

P

R

V

I

I

A

E

Q

R

I

L

S

G

G

G

Q

K

R

H

P

P

H

T

F

F

V

V

Q

E

G
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G

D
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D

I
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I

R
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R

D

N

R

E

A

A

V

L

L

M

D

T

G

E

I

I

F

L

K

H

S

D

H

H

R

A

I

I

E

D

A

T

V

V

I

I

H

H

F
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F

A
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A

G
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G

L
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L

K
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K

A

A

V

V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

D

D

N
|
N

N

N

V

V

H

N

G

G

S

T

N

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

A

A

H

A

G

N

V

V

K

K

N

N

I

F

V

I

F

F

S
|
S

A
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A

T
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T

V

V

Y

Y

G

G

D

D

P

N

A

P

S

K

V

I

P

P

I

Y

T

V

E

E

E

S

F

F

P

P

L

T

-

G

S

T

A

P

T

Q

N

S

P

P

Y
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Y

G

G

R

K

S

S

K
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K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

G

T

D

D

L

L

H

Q

V

K

A

A

D

Q

P

P

S

D

W

W

R

S

I

I

A

A

L

L

R

R

Y
|
Y

F
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F

N
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N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

I
x
M

G

G

E
|
E

D
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D

P
|
P

S

Q

G

G

I

I

P

P

N
|
N

L
|
L

L

M

P

P

F

Y

I

I

A

A

Q

Q

V

V

A

A

D

V

G

G

R

R

A

D

A

S

L
|
L

S
x
A

V
x
I

Y

F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

L

D

G

G

H

H

L

V

K

V

T

A

L

M

R

E

K

K

L

L

E

A

Q

N

G

K

P

P

G

G

V

V

Y

H

T

I

Y

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

N

N

S

S

V
|
V

L

L

E

D

M

V

L

V

A

N

A

A

F

F

E

S

Q

K

A

A

C

C

G

G

N

K

K

P

L

V

P

N

Y

Y

Q

H

L

F

V

A

D

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

C

A

C
x
Y

Y

W

A

A

A

D

T

A

E

S

R

K

A

A

E

D

R

R

E

E

L

L

G

N

W

W

R

R

A

V

Q

T

R

R

G

T

I

L

E

D

A

E

M

M

C

A

A

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

A

S

active site: S124 (= S127), A125 (= A128), T126 (= T129), Y149 (= Y151), K153 (= K155), M189 (≠ I191)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), S9 (≠ A12), G10 (= G13), Y11 (= Y14), I12 (= I15), D31 (= D34), N32 (= N35), L33 (= L36), C34 (= C37), N35 (= N38), S36 (= S39), G57 (= G60), D58 (= D61), I59 (= I62), R60 (= R63), F80 (= F83), A81 (= A84), G82 (= G85), L83 (= L86), K84 (= K87), N99 (= N102), S122 (= S125), S123 (= S126), S124 (= S127), Y149 (= Y151), K153 (= K155), Y177 (= Y179), F178 (= F180), P180 (= P182)
binding 1,3-propandiol: N35 (= N38), K84 (= K87), E191 (= E193), D192 (= D194), P193 (= P195)
binding uridine-5'-diphosphate: N179 (= N181), N198 (= N200), N199 (= N201), L200 (= L202), L215 (= L217), A216 (≠ S218), I217 (≠ V219), R231 (= R233), Y233 (= Y235), V269 (= V271), R292 (= R294), D295 (= D297), Y299 (≠ C301)

1lrjA Crystal structure of e. Coli udp-galactose 4-epimerase complexed with udp-n-acetylglucosamine (see paper)
59% identity, 95% coverage: 6:332/343 of query aligns to 3:330/338 of 1lrjA

query
sites
1lrjA

I

V

L

L

V

V

T

T

G
|
G

G

G

A
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

I

L

H

Q

A

N

G

G

Y

H

A

D

V

V

V

I

L

L

D
|
D

N
|
N

L
|
L

C
|
C

N
|
N

S
|
S

R

K

A

R

S

S

V

V

I

L

D

P

R

V

I

I

A

E

Q

R

I

L

S

G

G

G

Q

K

R

H

P

P

H

T

F

F

V

V

Q

E

G
|
G

D
|
D

I
|
I

R
|
R

D

N

R

E

A

A

V

L

L

M

D

T

G

E

I

I

F

L

K

H

S

D

H

H

R

A

I

I

E

D

A

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L
|
L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

D

D

N
|
N

N

N

V

V

H

N

G

G

S

T

N

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

A

A

H

A

G

N

V

V

K

K

N

N

I

F

V

I

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

P

N

A

P

S

K

V

I

P

P

I

Y

T

V

E

E

E

S

F

F

P

P

L

T

-

G

S

T

A

P

T

Q

N

S

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

G

T

D

D

L

L

H

Q

V

K

A

A

D

Q

P

P

S

D

W

W

R

S

I

I

A

A

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

I
x
M

G

G

E

E

D

D

P

P

S

Q

G

G

I

I

P

P

N
|
N

L
|
L

L

M

P

P

F
x
Y

I

I

A

A

Q

Q

V

V

A

A

D

V

G

G

R

R

A

D

A

S

L
|
L

S
x
A

V
x
I

Y
x
F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

L

D

G

G

H

H

L

V

K

V

T

A

L

M

R

E

K

K

L

L

E

A

Q

N

G

K

P

P

G

G

V

V

Y

H

T

I

Y

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

N

N

S

S

V
|
V

L

L

E

D

M

V

L

V

A

N

A

A

F

F

E

S

Q

K

A

A

C

C

G

G

N

K

K

P

L

V

P

N

Y

Y

Q

H

L

F

V

A

D

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

C

A

C
x
Y

Y

W

A

A

A

D

T

A

E

S

R

K

A

A

E

D

R

R

E

E

L

L

G

N

W

W

R

R

A

V

Q

T

R

R

G

T

I

L

E

D

A

E

M

M

C

A

A

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

A

S

active site: S124 (= S127), A125 (= A128), T126 (= T129), Y149 (= Y151), K153 (= K155), M189 (≠ I191)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), S9 (≠ A12), G10 (= G13), Y11 (= Y14), I12 (= I15), D31 (= D34), N32 (= N35), L33 (= L36), C34 (= C37), N35 (= N38), S36 (= S39), G57 (= G60), D58 (= D61), I59 (= I62), R60 (= R63), F80 (= F83), A81 (= A84), G82 (= G85), L83 (= L86), K84 (= K87), N99 (= N102), S122 (= S125), S123 (= S126), S124 (= S127), Y149 (= Y151), K153 (= K155), Y177 (= Y179), F178 (= F180), P180 (= P182), N199 (= N201)
binding uridine-diphosphate-n-acetylglucosamine: K84 (= K87), V86 (= V89), S124 (= S127), T126 (= T129), Y149 (= Y151), Y177 (= Y179), F178 (= F180), N179 (= N181), N198 (= N200), N199 (= N201), L200 (= L202), Y203 (≠ F205), L215 (= L217), A216 (≠ S218), I217 (≠ V219), F218 (≠ Y220), G229 (= G231), R231 (= R233), Y233 (= Y235), V269 (= V271), R292 (= R294), D295 (= D297), Y299 (≠ C301)

1kvrA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
59% identity, 95% coverage: 6:332/343 of query aligns to 3:330/338 of 1kvrA

query
sites
1kvrA

I

V

L

L

V

V

T

T

G
|
G

G

G

A
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

I

L

H

Q

A

N

G

G

Y

H

A

D

V

V

V

I

L

L

D
|
D

N
|
N

L
|
L

C
|
C

N
|
N

S
|
S

R

K

A

R

S

S

V

V

I

L

D

P

R

V

I

I

A

E

Q

R

I

L

S

G

G

G

Q

K

R

H

P

P

H

T

F

F

V

V

Q

E

G
|
G

D
|
D

I
|
I

R
|
R

D

N

R

E

A

A

V

L

L

M

D

T

G

E

I

I

F

L

K

H

S

D

H

H

R

A

I

I

E

D

A

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L
|
L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

D

D

N
|
N

N

N

V

V

H

N

G

G

S

T

N

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

A

A

H

A

G

N

V

V

K

K

N

N

I

F

V

I

F

F

S
|
S

S
x
A

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

P

Q

A

P

S

K

V

I

P

P

I

Y

T

V

E

E

E

S

F

F

P

P

L

T

-

G

S

T

A

P

T

Q

N

S

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

G

T

D

D

L

L

H

Q

V

K

A

A

D

Q

P

P

S

D

W

W

R

S

I

I

A

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

I
x
M

G

G

E

E

D

D

P

P

S

Q

G

G

I

I

P

P

N
|
N

L
|
L

L

M

P

P

F

Y

I

I

A

A

Q

Q

V

V

A

A

D

V

G

G

R

R

A

D

A

S

L
|
L

S
x
A

V
x
I

Y
x
F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

L

D

G

G

H

H

L

V

K

V

T

A

L

M

R

E

K

K

L

L

E

A

Q

N

G

K

P

P

G

G

V

V

Y

H

T

I

Y

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

N

N

S

S

V
|
V

L

L

E

D

M

V

L

V

A

N

A

A

F

F

E

S

Q

K

A

A

C

C

G

G

N

K

K

P

L

V

P

N

Y

Y

Q

H

L

F

V

A

D

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

C

A

C
x
Y

Y

W

A

A

A

D

T

A

E

S

R

K

A

A

E

D

R

R

E

E

L

L

G

N

W

W

R

R

A

V

Q

T

R

R

G

T

I

L

E

D

A

E

M

M

C

A

A

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

A

S

active site: A124 (≠ S127), A125 (= A128), T126 (= T129), Y149 (= Y151), K153 (= K155), M189 (≠ I191)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), S9 (≠ A12), G10 (= G13), Y11 (= Y14), I12 (= I15), D31 (= D34), N32 (= N35), L33 (= L36), C34 (= C37), N35 (= N38), S36 (= S39), G57 (= G60), D58 (= D61), I59 (= I62), R60 (= R63), F80 (= F83), A81 (= A84), G82 (= G85), L83 (= L86), K84 (= K87), N99 (= N102), S122 (= S125), S123 (= S126), A124 (≠ S127), Y149 (= Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding uridine-5'-diphosphate: N179 (= N181), N198 (= N200), N199 (= N201), L200 (= L202), L215 (= L217), A216 (≠ S218), I217 (≠ V219), F218 (≠ Y220), G229 (= G231), R231 (= R233), Y233 (= Y235), V269 (= V271), R292 (= R294), D295 (= D297), Y299 (≠ C301)

1a9yA Udp-galactose 4-epimerase mutant s124a/y149f complexed with udp- glucose (see paper)
59% identity, 95% coverage: 6:332/343 of query aligns to 3:330/338 of 1a9yA

query
sites
1a9yA

I

V

L

L

V

V

T

T

G
|
G

G

G

A
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

I

L

H

Q

A

N

G

G

Y

H

A

D

V

V

V

I

L

L

D
|
D

N
|
N

L
|
L

C
|
C

N
|
N

S
|
S

R

K

A

R

S

S

V

V

I

L

D

P

R

V

I

I

A

E

Q

R

I

L

S

G

G

G

Q

K

R

H

P

P

H

T

F

F

V

V

Q

E

G
|
G

D
|
D

I
|
I

R
|
R

D

N

R

E

A

A

V

L

L

M

D

T

G

E

I

I

F

L

K

H

S

D

H

H

R

A

I

I

E

D

A

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L
|
L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

D

D

N
|
N

N

N

V

V

H

N

G

G

S

T

N

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

A

A

H

A

G

N

V

V

K

K

N

N

I

F

V

I

F

F

S
|
S

S
x
A

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

P

Q

A

P

S

K

V

I

P

P

I

Y

T

V

E

E

E

S

F

F

P

P

L

T

-

G

S

T

A

P

T

Q

N

S

P

P

Y
x
F

G

G

R

K

S

S

K
|
K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

G

T

D

D

L

L

H

Q

V

K

A

A

D

Q

P

P

S

D

W

W

R

S

I

I

A

A

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

I

M

G

G

E

E

D

D

P

P

S

Q

G

G

I

I

P

P

N
|
N

L
|
L

L

M

P

P

F
x
Y

I

I

A

A

Q

Q

V

V

A

A

D

V

G

G

R

R

A

D

A

S

L
|
L

S
x
A

V
x
I

Y
x
F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

L

D

G

G

H

H

L

V

K

V

T

A

L

M

R

E

K

K

L

L

E

A

Q

N

G

K

P

P

G

G

V

V

Y

H

T

I

Y

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

N

N

S

S

V
|
V

L

L

E

D

M

V

L

V

A

N

A

A

F

F

E

S

Q

K

A

A

C

C

G

G

N

K

K

P

L

V

P

N

Y

Y

Q

H

L

F

V

A

D

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

C

A

C
x
Y

Y

W

A

A

A

D

T

A

E

S

R

K

A

A

E

D

R

R

E

E

L

L

G

N

W

W

R

R

A

V

Q

T

R

R

G

T

I

L

E

D

A

E

M

M

C

A

A

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

A

S

active site: A124 (≠ S127), A125 (= A128), T126 (= T129), F149 (≠ Y151), K153 (= K155)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), S9 (≠ A12), G10 (= G13), Y11 (= Y14), I12 (= I15), D31 (= D34), N32 (= N35), L33 (= L36), C34 (= C37), N35 (= N38), S36 (= S39), G57 (= G60), D58 (= D61), I59 (= I62), R60 (= R63), F80 (= F83), A81 (= A84), G82 (= G85), L83 (= L86), K84 (= K87), N99 (= N102), S122 (= S125), S123 (= S126), A124 (≠ S127), F149 (≠ Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding uridine-5'-diphosphate-glucose: A124 (≠ S127), A125 (= A128), T126 (= T129), F149 (≠ Y151), F178 (= F180), N179 (= N181), N198 (= N200), N199 (= N201), L200 (= L202), Y203 (≠ F205), L215 (= L217), A216 (≠ S218), I217 (≠ V219), F218 (≠ Y220), G229 (= G231), R231 (= R233), Y233 (= Y235), V269 (= V271), R292 (= R294), D295 (= D297), Y299 (≠ C301)

1hzjA Human udp-galactose 4-epimerase: accommodation of udp-n- acetylglucosamine within the active site (see paper)
57% identity, 95% coverage: 6:331/343 of query aligns to 4:336/345 of 1hzjA

query
sites
1hzjA

I

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

V

V

L

E

E

L

L

I

L

H

E

A

A

G

G

Y

Y

A

L

V

P

V

V

L

I

D
|
D

N
|
N

L
x
F

C
x
H

N
|
N

-
x
A

-

F

-

R

-

G

S

S

R

L

A

P

S

E

V

S

I

L

D

R

R

R

I

V

A

Q

Q

E

I

L

S

T

G

G

Q

R

R

S

P

V

H

E

F

F

V

E

Q

E

G
x
M

D
|
D

I
|
I

R

L

D

D

R

Q

A

G

V

A

L

L

D

Q

G

R

I

L

F

F

K

K

S

K

H

Y

R

S

I

F

E

M

A

A

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

D

Y

Y

D

R

N
x
V

N

N

V

L

H

T

G

G

S

T

N

I

V

Q

L

L

F

L

E

E

A

I

M

M

A

K

A

A

H

H

G

G

V

V

K

K

N

N

I

L

V

V

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

N

P

P

A

Q

S

Y

V

L

P

P

I

L

T

D

E

E

E

A

F

H

P

P

L

T

S

G

A

G

-

C

T

T

N

N

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

F

M

F

V

I

E

E

Q

E

I

M

L

I

G

R

D

D

L

L

H

C

V

Q

A

A

D

D

P

K

S

T

W

W

R

N

I

V

A

V

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

T

G

G

A

A

H

H

E

A

S

S

G

G

L

C

I

I

G

G

E

E

D

D

P

P

S

Q

G

G

I

I

P

P

N
|
N

L
|
L

L

M

P

P

F

Y

I

V

A

S

Q

Q

V

V

A

A

D

I

G

G

R

R

A

E

A

A

L
|
L

S
x
N

V
|
V

Y
x
F

G

G

S

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

L

K

G

G

H

H

L

I

K

A

T

A

L

L

R

R

K

K

L

L

E

K

Q

E

G

Q

P

C

G

G

V

C

Y

R

T

I

Y

Y

N

N

L

L

G

G

T

T

G

G

R

T

G

G

N

Y

S

S

V
|
V

L

L

E

Q

M

M

L

V

A

Q

A

A

F

M

E

E

Q

K

A

A

C

S

G

G

N

K

K

K

L

I

P

P

Y

Y

Q

K

L

V

V

V

D

A

R

R

R
|
R

P

E

G

G

D
|
D

I

V

A

A

C

A

C

C

Y

Y

A

A

A

N

T

P

E

S

R

L

A

A

E

Q

R

E

E

E

L

L

G

G

W

W

R

T

A

A

Q

A

R

L

G

G

I

L

E

D

A

R

M

M

C

C

A

E

D

D

T

L

W

W

R

R

W

W

Q

Q

active site: S131 (= S127), A132 (= A128), T133 (= T129), Y156 (= Y151), K160 (= K155)
binding nicotinamide-adenine-dinucleotide: G8 (= G10), G11 (= G13), Y12 (= Y14), I13 (= I15), D32 (= D34), N33 (= N35), F34 (≠ L36), H35 (≠ C37), N36 (= N38), A37 (vs. gap), M64 (≠ G60), D65 (= D61), I66 (= I62), F87 (= F83), A88 (= A84), G89 (= G85), K91 (= K87), V106 (≠ N102), S129 (= S125), S130 (= S126), S131 (= S127), Y156 (= Y151), K160 (= K155), Y184 (= Y179), P187 (= P182)
binding uridine-diphosphate-n-acetylglucosamine: V93 (= V89), T133 (= T129), Y156 (= Y151), N186 (= N181), N205 (= N200), N206 (= N201), L207 (= L202), L222 (= L217), N223 (≠ S218), V224 (= V219), F225 (≠ Y220), G236 (= G231), R238 (= R233), Y240 (= Y235), V276 (= V271), R299 (= R294), D302 (= D297)

6k0iA Crystal structure of udp-glucose 4-epimerase from bifidobacterium longum in complex with NAD+ and udp-glc (see paper)
57% identity, 96% coverage: 4:332/343 of query aligns to 1:329/335 of 6k0iA

query
sites
6k0iA

A

T

T

T

I

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

Y
x
F

I
|
I

G

A

S

T

H

H

T

T

C

D

V

I

E

E

L

L

I

L

H

N

A

K

G

G

Y

Y

A

D

V

V

V

I

V

S

L

V

D
|
D

N
|
N

L
x
Y

C
x
G

N
|
N

S
|
S

R

S

A

P

S

V

V

A

I

L

D

E

R

R

I

V

A

E

Q

Q

I

I

S

T

G

G

Q

K

R

P

P

V

H

K

F

R

V

Y

Q

D

G
|
G

D
|
D

I
x
V

R
|
R

D

D

R

E

A

A

V

L

L

M

D

E

G

R

I

V

F

F

K

A

S

E

H

N

R

N

I

I

E

D

A

W

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

A

A

Q

K

P

P

L

I

R

E

Y

Y

D

D

N
|
N

N

N

V

L

H

Y

G

S

S

T

N

L

V

V

L

L

F

L

E

K

A

V

M

M

A

K

H

H

G

N

V

V

K

K

N

K

I

I

V

I

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

T

P

P

A

K

S

E

V

L

P

P

I

I

-

T

T

E

E

E

E

T

F

P

P

T

L

G

S

G

A

T

T

T

N

N

P

P

Y
|
Y

G

G

R

T

S

S

K
|
K

L

L

M

F

V

Q

E

E

Q

Q

I

I

L

L

G

R

D

D

L

V

H

H

V

V

A

A

D

D

P

P

S

S

W

W

R

T

I

I

A

V

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

E

S

S

G

G

L

L

I

L

G

G

E

E

D

D

P

P

S

K

G

G

I

I

P

P

N
x
A

N
|
N

L
|
L

L

T

P

P

F

Y

I

V

A

A

Q

K

V

V

A

A

D

V

G

G

R

E

R

L

A

K

A

E

L

V

S
x
Q

V
|
V

Y
|
Y

G

G

S

D

D

D

Y

Y

P

D

T

T

P

P

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

L

K

G

G

H

H

L

V

K

A

T

V

L

I

R

D

K

H

L

I

E

D

Q

K

G

-

P

E

G

G

V

V

Y

F

T

V

Y

Y

N

N

L

L

G

G

T

T

G

G

R

H

G

G

N

Y

S

S

V
|
V

L

L

E

E

M

V

L

I

A

K

A

A

F

Y

E

E

Q

K

A

A

C

A

G

G

N

H

K

P

L

I

P

P

Y

Y

Q

A

L

I

V

K

D

P

R

R

R
|
R

P

P

G

G

D
|
D

I

I

A

A

C

A

C

C

Y

Y

A

A

A

D

T

A

E

S

R

K

A

A

E

E

R

K

E

E

L

L

G

G

W

W

R

K

A

A

Q

E

R

L

G

T

I

I

E

D

A

D

M

M

C

A

A

A

D

S

T

S

W

L

R

N

W

W

Q

Q

A

T

active site: S124 (= S127), A125 (= A128), T126 (= T129), Y149 (= Y151), K153 (= K155)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (≠ Y14), I12 (= I15), D31 (= D34), N32 (= N35), Y33 (≠ L36), G34 (≠ C37),