SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS10775 HSERO_RS10775 cysteine ABC transporter substrate-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
43% identity, 83% coverage: 37:257/267 of query aligns to 4:224/225 of 4zv2A

query
sites
4zv2A

G

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binding glutamine: E11 (= E44), F14 (≠ Y47), F52 (≠ W86), G70 (≠ Q104), M71 (≠ V105), T72 (≠ G106), R77 (= R111), S120 (≠ T155), T121 (≠ N156), D159 (= D193)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
42% identity, 83% coverage: 37:257/267 of query aligns to 4:226/226 of 4zv1A

query
sites
4zv1A

G

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binding arginine: E11 (= E44), F14 (≠ Y47), F52 (≠ W86), A69 (≠ N103), G70 (≠ Q104), M71 (≠ V105), T72 (≠ G106), R77 (= R111), Q117 (≠ G152), S120 (≠ T155), T121 (≠ N156), D161 (= D193)

3vv5A Crystal structure of ttc0807 complexed with (s)-2-aminoethyl-l- cysteine (aec) (see paper)
37% identity, 87% coverage: 30:260/267 of query aligns to 6:234/237 of 3vv5A

query
sites
3vv5A

L

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binding l-thialysine: E20 (= E44), F23 (≠ Y47), N27 (= N51), F61 (≠ W86), A78 (≠ N103), S79 (≠ Q104), G81 (= G106), R86 (= R111), T127 (= T155), T128 (≠ N156), Y129 (≠ F157)

3vvfA Crystal structure of ttc0807 complexed with arginine (see paper)
37% identity, 87% coverage: 30:260/267 of query aligns to 10:238/241 of 3vvfA

query
sites
3vvfA

L

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K

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E

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E

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N

N

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D

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A

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L

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R

A

A

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L

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A

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binding arginine: E24 (= E44), F27 (≠ Y47), F65 (≠ W86), A82 (≠ N103), S83 (≠ Q104), H84 (≠ V105), G85 (= G106), R90 (= R111), Q128 (≠ G152), T131 (= T155), T132 (≠ N156), Y133 (≠ F157), E196 (≠ D218)

3vveA Crystal structure of ttc0807 complexed with lysine (see paper)
37% identity, 87% coverage: 30:260/267 of query aligns to 10:238/241 of 3vveA

query
sites
3vveA

L

F

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F

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E

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A

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D

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E

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K

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G

N

N

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L

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L

A

D

A

A

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L

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N

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A

A

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binding lysine: E24 (= E44), F27 (≠ Y47), F65 (≠ W86), S83 (≠ Q104), H84 (≠ V105), R90 (= R111), Q128 (≠ G152), T131 (= T155), T132 (≠ N156), Y133 (≠ F157), E196 (≠ D218)

3vvdA Crystal structure of ttc0807 complexed with ornithine (see paper)
37% identity, 87% coverage: 30:260/267 of query aligns to 10:238/241 of 3vvdA

query
sites
3vvdA

L

F

Q

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S

E

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E

V

V

D

D

V

L

A

G

K

N

L

A

L

I

A

A

A

E

K

R

L

L

G

G

V

L

K

K

P

P

V

R

F

W

T

I

T

A

T

Q

E

S

W
x
F

S

D

G

T

I

L

L

L

A

I

G

Q

L

L

G

N

A

Q

G

G

K

R

Y

F

D

D

V

F

I

V

I

I

N

A

Q
x
S

V
x
H

G
|
G

I

I

T

T

D

E

E

E

R
|
R

Q

A

K

R

A

A

F

V

D

D

F

F

S

T

D

N

P

P

Y

H

T

Y

L

C

S

T

S

G

A

G

Q

V

L

I

I

V

V

S

R

R

K

K

D

G

E

G

K

P

R

R

E

T

F

A

K

K

S

-

L

-

E

-

D

D

L

L

K

Q

G

G

K

K

K

V

L

V

G

G

L

V

G
x
Q

Q

V

G

G

T
|
T

N
x
T

F
x
Y

E

M

Q

E

K

A

A

A

K

Q

A

K

V

I

P

P

G

G

I

I

-

K

D

E

V

V

R

R

T

T

Y

Y

P

Q

G

R

S

D

P

P

E

D

Y

A

L

L

A

Q

D

D

L

L

A

L

A

A

G

G

R

R

I

I

D

D

A

T

A

W

L

I

N

T

D

D

S

R

L

F

L

V

V

A

G

K

Y

E

I

A

L

I

K

K

S

E

T

R

N

K

L

L

P

E

-

N

-

T

L

L

K

Q

A

V

G

G

S

E

P

L

I

V

G

F

A

Q

V

-

D
x
E

K

R

I

V

G

A

I

M

P

A

F

V

R

A

K

K

G

G

N

N

P

K

E

S

F

L

K

L

A

D

A

A

L

L

N

N

K

R

A

A

L

L

A

A

D

E

I

L

K

M

A

Q

D

D

G

G

S

T

F

Y

K

A

A

R

A

I

S

S

E

Q

K

K

W

W

F

F

G

G

I

E

D

D

V

V

binding L-ornithine: E24 (= E44), F65 (≠ W86), S83 (≠ Q104), H84 (≠ V105), G85 (= G106), R90 (= R111), Q128 (≠ G152), T131 (= T155), T132 (≠ N156), Y133 (≠ F157), E196 (≠ D218)

5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
38% identity, 85% coverage: 30:256/267 of query aligns to 3:228/229 of 5t0wA

query
sites
5t0wA

L

L

Q

D

S

E

V

I

K

M

S

K

S

R

G

G

T

T

L

L

K

R

V

V

A

G

L

T

E
x
D

G

A

N

D

Y
|
Y

P

K

P

P

F

F

N

S

F

F

K

K

D

D

P

-

K

K

T

N

G

G

E

Q

L

Y

T

T

G

G

F

F

E

D

V

I

D

D

V

L

A

A

K

K

L

A

L

L

A

A

A

K

K

E

L

L

G

G

V

V

K

K

P

V

V

E

F

F

T

V

T

P

T

T

E

T

W
|
W

S

D

G

G

I

I

L

I

A

P

G

A

L

L

G

Q

A

T

G

G

K

K

Y

F

D

D

V

I

I

V

I

M

N
x
S

Q
x
G

V
x
M

G
x
T

I

I

T

T

D

P

E

E

R
|
R

Q

K

K

K

A

K

F

V

D

D

F

F

S

S

D

D

P

P

Y

Y

T

M

L

T

S

A

S

G

A

Q

Q

T

L

I

I

L

V

V

R

K

K

K

D

D

E

N

K

A

R

D

E

K

F

I

K

K

S

S

L

F

E

E

D

D

L

L

K

N

G

K

K

P

-

D

-

V

K

K

L

V

G

A

L

V

G
x
Q

Q

L

G

G

T
|
T

N
x
T

F

S

E

E

Q

Q

K

A

A

A

K

K

A

E

-

F

V

L

P

P

G

K

I

A

D

K

V

I

R

R

T

T

Y

F

P

E

G

N

S

N

P

A

E

E

Y

A

L

F

A

Q

D

E

L

V

A

V

A

S

G

G

R

R

I

A

D

D

A

A

A

M

L

V

N

T

D
|
D

S

S

L

P

L

V

V

A

G

A

Y

Y

I

Y

L

A

K

K

S

-

T

-

N

-

L

L

P

A

L

V

K

V

A

V

G

V

S

D

P

E

I

P

G

F

A

T

V

H

D

E

K

P

I

L

G

G

I

F

P

A

F

I

R

R

K

K

G

G

N

D

P

P

E

E

F

L

K

L

A

N

A

W

L

V

N

N

K

N

A

W

L

L

A

K

D

Q

I

M

K

K

A

K

D

D

G

G

S

T

F

Y

K

D

A

K

A

L

S

Y

E

E

K

K

W

W

F

F

binding arginine: D17 (≠ E44), Y20 (= Y47), W58 (= W86), S75 (≠ N103), G76 (≠ Q104), M77 (≠ V105), T78 (≠ G106), R83 (= R111), Q126 (≠ G152), T129 (= T155), T130 (≠ N156), D168 (= D193)

2ylnA Crystal structure of the l-cystine solute receptor of neisseria gonorrhoeae in the closed conformation (see paper)
40% identity, 87% coverage: 29:261/267 of query aligns to 6:238/240 of 2ylnA

query
sites
2ylnA

L

L

L

I

Q

E

S

R

V

I

K

N

S

N

S

K

G

G

T

T

L

V

K

T

V

V

A

G

L

T

E
|
E

G

G

N

T

Y
|
Y

P

A

P

P

F

F

N

T

F

Y

K

H

D

D

P

-

K

K

T

D

G

G

E

K

L

L

T

T

G

G

F

Y

E

D

V

V

D

E

V

V

A

T

K

R

L

A

L

V

A

A

A

E

K

K

L

L

G

G

V

V

K

K

P

V

V

E

F

F

T

K

T

E

T

T

E

Q

W
|
W

S

D

G

S

I

M

L

M

A

A

G

G

L

L

G

K

A

A

G

G

K

R

Y

F

D

D

V

V

I

V

I

A

N
|
N

Q

Q

V

V

G

G

I

L

T

T

D

S

-

P

E

E

R
|
R

Q

Q

K

A

A

T

F

F

D

D

F

K

S

S

D

E

P

P

Y

Y

T

S

L

W

S

S

S

G

A

A

Q

V

L

L

I

V

V

A

R

H

K

N

D

D

E

S

K

N

R

-

E

-

F

I

K

K

S

S

L

I

E

A

D

D

L

I

K

K

G

G

K

V

K

K

L

T

G

A

L

Q

G
x
S

Q

L

G

T

T
x
S

N
|
N

F

Y

E

G

Q

E

K

K

A

A

K

K

A

A

V

-

P

A

G

G

I

A

D

Q

V

L

R

V

T

P

Y

V

P

D

G
|
G

S
x
L

P

A

E

Q

Y

S

L

L

A

T

D

L

L

I

A

E

A

Q

G

K

R

R

I

A

D

D

A

A

A

T

L

L

N
|
N

D

D

S

E

L

L

L

A

V

V

-

L

G

D

Y

Y

I

L

L

K

K

K

S

N

T

P

N

N

L

A

P

G

L

V

K

K

A

I

G

V

S

W

P

S

I

A

G

P

A

A

V

D

D

E

K

K

I

V

-

G

-

S

G

G

I

L

P

I

F

V

R

N

K

K

G

G

N

N

P

D

E

E

F

A

K

V

A

A

A

K

L

F

N

S

K

T

A

A

L

I

A

N

D

E

I

L

K

K

A

A

D

D

G

G

S

T

F

L

K

K

A

K

A

L

S

G

E

E

K

Q

W

F

F

F

G

G

I

K

D

D

V

I

S

S

binding cysteine: E21 (= E44), Y24 (= Y47), W62 (= W86), W62 (= W86), N79 (= N103), R88 (= R111), S127 (≠ G152), S130 (≠ T155), N131 (= N156), G148 (= G174), L149 (≠ S175), N166 (= N192)

P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
32% identity, 84% coverage: 38:260/267 of query aligns to 27:257/260 of P0AEU0

query
sites
P0AEU0

T

N

L

I

K

R

V

I

A

G

L

T

E

D

G

P

N

T

Y

Y

P

A

P

P

F

F

N

E

F

S

K

K

D

N

P

S

K

Q

T

-

G

G

E

E

L

L

T

V

G

G

F

F

E

D

V

I

D

D

V

L

A

A

K

K

L

E

L

L

A
x
C

A

K

K

R

L

I

G

N

V

T

K

Q

P
x
C

V

T

F

F

T

V

T

E

T

N

E

P

W

L

S

D

G

A

I

L

L

I

A

P

G

S

L

L

G

K

A

A

G

K

K

K

Y

I

D

D

V

A

I

I

I

M

N
x
S

Q
x
S

V

L

G
x
S

I

I

T

T

D

E

E

K

R
|
R

Q

Q

K

Q

A

E

F

I

D

A

F

F

S

T

D

D

P

K

Y

L

T

Y

L

A

S

A

S

D

A

S

Q

R

L

L

I

V

V

V

R

A

K

K

D

N

E

S

K

D

R

I

E

Q

F

-

K

P

S

T

L

V

E

E

D

S

L

L

K

K

G

G

K

K

K

R

L

V

G

G

L

V

G

L

Q

Q

G

G

T

T

-
x
T

-

Q

-

E

N

T

F

F

E

G

Q

N

K

E

A

H

K

W

A

A

V

P

P

K

G

G

I

I

D

E

V

I

R

V

T

S

Y

Y

P

Q

G

G

S

Q

P

D

E

N

Y

I

L

Y

A

S

D

D

L

L

A

T

A

A

G

G

R

R

I

I

D

D

A

A

L

F

N

Q

D
|
D

S

E

L

V

L

A

V

A

-

S

-

E

G

G

Y

F

I

L

L

K

K

Q

S

P

T

V

N

G

L

K

P

D

L

Y

K

K

A

F

G

G

S

G

P

P

I

S

G

V

A

K

V

D

D

E

K

K

I

L

-

F

-

G

-

V

-

G

-

T

G

G

I

M

P

G

F

L

R

R

K

K

G

E

N

D

P

N

E

E

F

L

K

R

A

E

A

A

L

L

N

N

K

K

A

A

L

F

A

A

D

E

I

M

K

R

A

A

D

D

G

G

S

T

F

Y

K

E

A

K

A

L

S

A

E

K

K

K

W

Y

F

F

G

D

I

F

D

D

V

V

C60 (≠ A72) modified: Disulfide link with 67
C67 (≠ P79) modified: Disulfide link with 60
S91 (≠ N103) binding
S92 (≠ Q104) binding
S94 (≠ G106) binding
R99 (= R111) binding
T143 (vs. gap) binding
D183 (= D193) binding

Sites not aligning to the query:

1:22 signal peptide

6svfA Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
33% identity, 85% coverage: 30:256/267 of query aligns to 3:227/229 of 6svfA

query
sites
6svfA

L

I

Q

D

S

E

V

I

K

K

S

S

S

R

G

G

T

Y

L

L

K

L

V

V

A

G

L

L

E

S

G

A

N

D

Y
x
F

P

P

F

F

N

E

F

F

K

V

D

D

P

-

K

E

T

N

G

G

E

N

L

I

T

V

G

G

F

F

E

D

V

V

D

D

V

L

A

A

K

K

L

E

L

I

A

A

A

R

K

R

L

L

G

G

V

V

K

E

P

L

V

K

F

I

T

V

T

D

T

M

E

T

W
x
F

S

D

G

G

I

L

L

I

A

P

G

S

L

L

G

L

A

T

G

K

K

K

Y

I

D

D

V

V

I

I

N
x
S

Q
x
G

V
x
M

G
x
T

I

I

T

T

D

E

E

E

R
|
R

Q

K

K

K

A

V

F

V

D

A

F

F

S

S

D

D

P

P

Y

Y

T

F

L

D

S

A

S

G

A

Q

Q

V

L

I

I

V

V

V

R

R

K

K

D

D

E

S

K

D

R

F

E

R

F

P

K

K

S

T

L

Y

E

E

D

D

L

L

K

V

G

G

K

K

K

T

L

V

G

A

L

V

G
x
Q

Q

I

G

G

T
|
T

N
x
T

F

G

E

D

Q

I

K

E

A

V

K

S

A

K

V

Y

P

D

G

G

I

I

D

K

V

V

R

V

T

R

Y

F

P

D

G

K

S

F

P

T

E

D

Y

A

L

F

A

L

D

E

L

L

A

K

A

R

G

G

R

R

I

A

D

D

A

A

A

V

L

V

N

L

D
|
D

S

S

L

A

L

T

V

A

-

R

G

A

Y

F

I

V

L

A

K

K

S

N

T

P

N

D

L

L

P

V

L

I

K

S

A

S

G

G

S

-

P

-

I

V

G

L

A

S

V

S

D

E

K

Q

I

Y

G

G

I

I

P

A

F

V

R

R

K

K

G

E

N

D

P

T

E

D

F

L

K

L

A

E

A

F

L

I

N

N

K

S

A

V

L

L

A

R

D

E

I

L

K

K

A

K

D

S

G

G

S

K

F

Y

K

D

A

V

A

L

S

I

E

E

K

K

W

W

F

F

binding arginine: F20 (≠ Y47), F58 (≠ W86), S75 (≠ N103), G76 (≠ Q104), M77 (≠ V105), T78 (≠ G106), R83 (= R111), Q124 (≠ G152), T127 (= T155), T128 (≠ N156), D165 (= D193)

P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
32% identity, 83% coverage: 39:260/267 of query aligns to 28:257/260 of P02910

query
sites
P02910

L

I

K

R

V

I

A

G

L

T

E

D

G

P

N

T

Y

Y

P

A

P

P

F

F

N
x
E

F

S

K
|
K

D

N

P

A

K

Q

T

-

G

G

E
|
E

L

L

T

V

G

G

F

F

E

D

V

I

D

D

V

L

A

A

K

K

L

E

L

L

A

C

A

K

K

R

L

I

G

N

V

T

K

Q

P

C

V

T

F

F

T

V

T

E

T

N

E

P

W

L

S

D

G

A

I

L

L

I

A

P

G

S

L

L

G

K

A

A

G

K

K

K

Y

I

D

D

V

A

I

I

I

M

N

S

Q

S

V

L

G

S

I

I

T

T

D

E

E

K

R

R

Q

Q

K

Q

A

E

F

I

D

A

F

F

S

T

D

D

P

K

Y

L

T

Y

L

A

S

A

S

D

A

S

Q

R

L

L

I

V

V

V

R

A

K

K

D

N

E

S

K

D

R

I

E

Q

F

-

K

P

S

T

L

V

E

A

D

S

L

L

K

K

G

G

K

K

K

R

L

V

G

G

L

V

G

L

Q

Q

G

G

T

T

-

T

-

Q

-

E

N

T

F

F

E

G

Q

N

K

E

A

H

K

W

A

A

V

P

P

K

G

G

I

I

D

E

V

I

R

V

T

S

Y

Y

P

Q

G

G

S

Q

P

D

E

N

Y

I

L

Y

A

S

D
|
D

L

L

A

T

A

A

G

G

R
|
R

I

I

D
|
D

A

A

L

F

N

Q

D

D

S

E

L

V

L

A

V

A

-

S

-

E

G

G

Y

F

I

L

L

K

K

Q

S

P

T

V

N

G

L

K

P

D

L

Y

K

K

A

F

G

G

S

G

P

P

I

A

G

V

A

K

V

D

D

E

K

K

I

L

-

F

-

G

-

V

-

G

-

T

G

G

I

M

P

G

F

L

R

R

K

K

G

E

N

D

P

N

E

E

F

L

K

R

A

E

A

A

L

L

N

N

K

K

A

A

L

F

A

A

D

E

I

M

K

R

A

A

D

D

G

G

S

T

F

Y

K

E

A

K

A

L

S

A

E

K

K

K

W

Y

F

F

G

D

I

F

D

D

V

V

E40 (≠ N51) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
K42 (= K53) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
E47 (= E59) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
D171 (= D181) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
R176 (= R186) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
D178 (= D188) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.

Sites not aligning to the query:

1:22 signal peptide

1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
32% identity, 83% coverage: 39:260/267 of query aligns to 6:235/238 of 1hslA

query
sites
1hslA

L

I

K

R

V

I

A

G

L

T

E

D

G

P

N

T

Y
|
Y

P

A

P

P

F

F

N

E

F

S

K

K

D

N

P

A

K

Q

T

-

G

G

E

E

L

L

T

V

G

G

F

F

E

D

V

I

D

D

V

L

A

A

K

K

L

E

L

L

A

C

A

K

K

R

L

I

G

N

V

T

K

Q

P

C

V

T

F

F

T

V

T

E

T

N

E

P

W
x
L

S

D

G

A

I

L

L

I

A

P

G

S

L

L

G

K

A

A

G

K

K

K

Y

I

D

D

V

A

I

I

I

M

N
x
S

Q
x
S

V
x
L

G
x
S

I

I

T

T

D

E

E

K

R
|
R

Q

Q

K

Q

A

E

F

I

D

A

F

F

S

T

D

D

P

K

Y

L

T

Y

L

A

S

A

S

D

A

S

Q

R

L

L

I

V

V

V

R

A

K

K

D

N

E

S

K

D

R

I

E

Q

F

-

K

P

S

T

L

V

E

A

D

S

L

L

K

K

G

G

K

K

K

R

L

V

G

G

L

V

G

L

Q

Q

G

G

T
|
T

-
x
T

-

Q

-

E

N

T

F

F

E

G

Q

N

K

E

A

H

K

W

A

A

V

P

P

K

G

G

I

I

D

E

V

I

R

V

T

S

Y

Y

P

Q

G

G

S

Q

P

D

E

N

Y

I

L

Y

A

S

D

D

L

L

A

T

A

A

G

G

R

R

I

I

D

D

A

A

L

F

N

Q

D
|
D

S

E

L

V

L

A

V

A

-

S

-

E

G

G

Y

F

I

L

L

K

K

Q

S

P

T

V

N

G

L

K

P

D

L

Y

K

K

A

F

G

G

S

G

P

P

I

A

G

V

A

K

V

D

D

E

K

K

I

L

-

F

-

G

-

V

-

G

-

T

G

G

I

M

P

G

F

L

R

R

K

K

G

E

N

D

P

N

E

E

F

L

K

R

A

E

A

A

L

L

N

N

K

K

A

A

L

F

A

A

D

E

I

M

K

R

A

A

D

D

G

G

S

T

F

Y

K

E

A

K

A

L

S

A

E

K

K

K

W

Y

F

F

G

D

I

F

D

D

V

V

binding histidine: Y14 (= Y47), L52 (≠ W86), S69 (≠ N103), S70 (≠ Q104), L71 (≠ V105), S72 (≠ G106), R77 (= R111), T120 (= T155), T121 (vs. gap), D161 (= D193)

P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
32% identity, 84% coverage: 38:260/267 of query aligns to 27:257/260 of P02911

query
sites
P02911

T

T

L

V

K

R

V

I

A

G

L

T

E
x
D

G

T

N

T

Y
|
Y

P

A

P

P

F

F

N

S

F

S

K

K

D

D

P

A

K

K

T

-

G

G

E

E

L

F

T

I

G

G

F

F

E
x
D

V

I

D

D

V

L

A

G

K

N

L

E

L

M

A
x
C

A

K

K

R

L

M

G

Q

V

V

K

K

P
x
C

V

T

F

W

T

V

T

A

T

S

E

D

W
x
F

S

D

G

A

I

L

L

I

A

P

G

S

L

L

G

K

A

A

G

K

K

K

Y

I

D

D

V

A

I

I

N
x
S

Q
x
S

V

L

G
x
S

I

I

T

T

D

D

E

K

R
|
R

Q

Q

K

Q

A

E

F

I

D

A

F

F

S

S

D

D

P

K

Y

L

T

Y

L

A

S

A

S

D

A

S

Q

R

L

L

I

I

V

A

R

A

K

K

D

G

E

S

K

P

R

I

E

Q

F

-

K

P

S

T

L

L

E

E

D

S

L

L

K

K

G

G

K

K

K

H

L

V

G

G

L

V

G
x
L

Q

Q

G

G

T

S

N
x
T

F

Q

E

E

Q

A

K

Y

A

A

K

N

-

D

-

N

-

W

A

R

V

T

P

K

G

G

I

V

D

D

V

V

R

V

T

A

Y

Y

P

A

G

N

S

Q

P

D

E

L

Y

I

L

Y

A

S

D

D

L

L

A

T

A

A

G

G

R

R

I

L

D

D

A

A

L

L

N

Q

D
|
D

S

E

L

V

L

A

V

A

-

S

-

E

G

G

Y

F

I

L

L

K

K

Q

S

P

T

A

N

G

L

K

P

E

L

Y

K

A

A

F

G

A

S

G

P

P

-

S

-

V

-

K

-

D

-

K

-

Y

I

F

G

G

A

-

V

-

D

D

K

G

I

T

G

G

I

V

P

G

F

L

R

R

K

K

G

D

N

D

P

T

E

E

F

L

K

K

A

A

L

F

N

D

K

K

A

A

L

L

A

T

D

E

I

L

K

R

A

Q

D

D

G

G

S

T

F

Y

K

D

A

K

A

M

S

A

E

K

K

K

W

Y

F

F

G

D

I

F

D

N

V

V

D33 (≠ E44) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
Y36 (= Y47) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
D52 (≠ E64) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
C60 (≠ A72) modified: Disulfide link with 67
C67 (≠ P79) modified: Disulfide link with 60
F74 (≠ W86) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
S91 (≠ N103) binding ; binding ; mutation to A: Has no effect on the arginine and histidine binding affinity.
S92 (≠ Q104) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
S94 (≠ G106) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
R99 (= R111) binding ; binding ; binding ; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
L139 (≠ G152) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
T143 (≠ N156) binding ; binding ; binding
D183 (= D193) binding ; binding ; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.

5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
32% identity, 84% coverage: 38:260/267 of query aligns to 2:232/235 of 5owfA

query
sites
5owfA

T

T

L

V

K

R

V

I

A

G

L

T

E

D

G

T

N

T

Y
|
Y

P

A

P

P

F

F

N

S

F

S

K

K

D

D

P

A

K

K

T

-

G

G

E

E

L

F

T

I

G

G

F

F

E

D

V

I

D

D

V

L

A

G

K

N

L

E

L

M

A

C

A

K

K

R

L

M

G

Q

V

V

K

K

P

C

V

T

F

W

T

V

T

A

T

S

E

D

W
x
F

S

D

G

A

I

L

L

I

A

P

G

S

L

L

G

K

A

A

G

K

K

K

Y

I

D

D

V

A

I

I

N
x
S

Q
x
S

V

L

G
x
S

I

I

T

T

D

D

E

K

R
|
R

Q

Q

K

Q

A

E

F

I

D

A

F

F

S

S

D

D

P

K

Y

L

T

Y

L

A

S

A

S

D

A

S

Q

R

L

L

I

I

V

A

R

A

K

K

D

G

E

S

K

P

R

I

E

Q

F

-

K

P

S

T

L

L

E

E

D

S

L

L

K

K

G

G

K

K

K

H

L

V

G

G

L

V

G
x
K

Q

Q

G

G

T
x
S

N
x
T

F

Q

E

E

Q

A

K

Y

A

A

K

N

-

D

-

N

-

W

A

R

V

T

P

K

G

G

I

V

D

D

V

V

R

V

T

A

Y

Y

P

A

G

N

S

Q

P

D

E

L

Y

I

L

Y

A

S

D

D

L

L

A

T

A

A

G

G

R

R

I

L

D

D

A

A

L

L

N

Q

D
|
D

S

E

L

V

L

A

V

A

-

S

-

E

G

G

Y

F

I

L

L

K

K

Q

S

P

T

A

N

G

L

K

P

E

L

Y

K

A

A

F

G

A

S

G

P

P

-

S

-

V

-

K

-

D

-

K

-

Y

I

F

G

G

A

-

V

-

D

D

K

G

I

T

G

G

I

V

P

G

F

L

R

R

K

K

G

D

N

D

P

T

E

E

F

L

K

K

A

A

L

F

N

D

K

K

A

A

L

L

A

T

D

E

I

L

K

R

A

Q

D

D

G

G

S

T

F

Y

K

D

A

K

A

M

S

A

E

K

K

K

W

Y

F

F

G

D

I

F

D

N

V

V

binding glutamine: Y11 (= Y47), F49 (≠ W86), S66 (≠ N103), S67 (≠ Q104), S69 (≠ G106), R74 (= R111), K114 (≠ G152), S117 (≠ T155), T118 (≠ N156), D158 (= D193)

1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
32% identity, 84% coverage: 38:260/267 of query aligns to 5:235/238 of 1lstA

query
sites
1lstA

T

T

L

V

K

R

V

I

A

G

L

T

E

D

G

T

N

T

Y
|
Y

P

A

P

P

F

F

N

S

F

S

K

K

D

D

P

A

K

K

T

-

G

G

E

E

L

F

T

I

G

G

F

F

E

D

V

I

D

D

V

L

A

G

K

N

L

E

L

M

A

C

A

K

K

R

L

M

G

Q

V

V

K

K

P

C

V

T

F

W

T

V

T

A

T

S

E

D

W
x
F

S

D

G

A

I

L

L

I

A

P

G

S

L

L

G

K

A

A

G

K

K

K

Y

I

D

D

V

A

I

I

N

S

Q
x
S

V

L

G
x
S

I

I

T

T

D

D

E

K

R
|
R

Q

Q

K

Q

A

E

F

I

D

A

F

F

S

S

D

D

P

K

Y

L

T

Y

L

A

S

A

S

D

A

S

Q

R

L

L

I

I

V

A

R

A

K

K

D

G

E

S

K

P

R

I

E

Q

F

-

K

P

S

T

L

L

E

E

D

S

L

L

K

K

G

G

K

K

K

H

L

V

G

G

L

V

G
x
L

Q

Q

G

G

T
x
S

N
x
T

F
x
Q

E

E

Q

A

K

Y

A

A

K

N

-

D

-

N

-

W

A

R

V

T

P

K

G

G

I

V

D

D

V

V

R

V

T

A

Y

Y

P

A

G

N

S

Q

P

D

E

L

Y

I

L

Y

A

S

D

D

L

L

A

T

A

A

G

G

R

R

I

L

D

D

A

A

L

L

N

Q

D
|
D

S

E

L

V

L

A

V

A

-

S

-

E

G

G

Y

F

I

L

L

K

K

Q

S

P

T

A

N

G

L

K

P

E

L

Y

K

A

A

F

G

A

S

G

P

P

-

S

-

V

-

K

-

D

-

K

-

Y

I

F

G

G

A

-

V

-

D

D

K

G

I

T

G

G

I

V

P

G

F

L

R

R

K

K

G

D

N

D

P

T

E

E

F

L

K

K

A

A

L

F

N

D

K

K

A

A

L

L

A

T

D

E

I

L

K

R

A

Q

D

D

G

G

S

T

F

Y

K

D

A

K

A

M

S

A

E

K

K

K

W

Y

F

F

G

D

I

F

D

N

V

V

binding lysine: Y14 (= Y47), F52 (≠ W86), S70 (≠ Q104), S72 (≠ G106), R77 (= R111), L117 (≠ G152), S120 (≠ T155), T121 (≠ N156), Q122 (≠ F157), D161 (= D193)

1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
32% identity, 84% coverage: 38:260/267 of query aligns to 5:235/238 of 1lahE

query
sites
1lahE

T

T

L

V

K

R

V

I

A

G

L

T

E

D

G

T

N

T

Y
|
Y

P

A

P

P

F

F

N

S

F

S

K

K

D

D

P

A

K

K

T

-

G

G

E

E

L

F

T

I

G

G

F

F

E

D

V

I

D

D

V

L

A

G

K

N

L

E

L

M

A

C

A

K

K

R

L

M

G

Q

V

V

K

K

P

C

V

T

F

W

T

V

T

A

T

S

E

D

W
x
F

S

D

G

A

I

L

L

I

A

P

G

S

L

L

G

K

A

A

G

K

K

K

Y

I

D

D

V

A

I

I

N

S

Q
x
S

V

L

G
x
S

I

I

T

T

D

D

E

K

R
|
R

Q

Q

K

Q

A

E

F

I

D

A

F

F

S

S

D

D

P

K

Y

L

T

Y

L

A

S

A

S

D

A

S

Q

R

L

L

I

I

V

A

R

A

K

K

D

G

E

S

K

P

R

I

E

Q

F

-

K

P

S

T

L

L

E

E

D

S

L

L

K

K

G

G

K

K

K

H

L

V

G

G

L

V

G

L

Q

Q

G

G

T
x
S

N

T

F
x
Q

E

E

Q

A

K

Y

A

A

K

N

-

D

-

N

-

W

A

R

V

T

P

K

G

G

I

V

D

D

V

V

R

V

T

A

Y

Y

P

A

G

N

S

Q

P

D

E

L

Y

I

L

Y

A

S

D

D

L

L

A

T

A

A

G

G

R

R

I

L

D

D

A

A

L

L

N

Q

D
|
D

S

E

L

V

L

A

V

A

-

S

-

E

G

G

Y

F

I

L

L

K

K

Q

S

P

T

A

N

G

L

K

P

E

L

Y

K

A

A

F

G

A

S

G

P

P

-

S

-

V

-

K

-

D

-

K

-

Y

I

F

G

G

A

-

V

-

D

D

K

G

I

T

G

G

I

V

P

G

F

L

R

R

K

K

G

D

N

D

P

T

E

E

F

L

K

K

A

A

L

F

N

D

K

K

A

A

L

L

A

T

D

E

I

L

K

R

A

Q

D

D

G

G

S

T

F

Y

K

D

A

K

A

M

S

A

E

K

K

K

W

Y

F

F

G

D

I

F

D

N

V

V

binding L-ornithine: Y14 (= Y47), F52 (≠ W86), S70 (≠ Q104), S72 (≠ G106), R77 (= R111), S120 (≠ T155), Q122 (≠ F157), D161 (= D193)

1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
32% identity, 84% coverage: 38:260/267 of query aligns to 5:235/238 of 1lagE

query
sites
1lagE

T

T

L

V

K

R

V

I

A

G

L

T

E

D

G

T

N

T

Y
|
Y

P

A

P

P

F

F

N

S

F

S

K

K

D

D

P

A

K

K

T

-

G

G

E

E

L

F

T

I

G

G

F

F

E

D

V

I

D

D

V

L

A

G

K

N

L

E

L

M

A

C

A

K

K

R

L

M

G

Q

V

V

K

K

P

C

V

T

F

W

T

V

T

A

T

S

E

D

W
x
F

S

D

G

A

I

L

L

I

A

P

G

S

L

L

G

K

A

A

G

K

K

K

Y

I

D

D

V

A

I

I

N
x
S

Q
x
S

V
x
L

G
x
S

I

I

T

T

D

D

E

K

R
|
R

Q

Q

K

Q

A

E

F

I

D

A

F

F

S

S

D

D

P

K

Y

L

T

Y

L

A

S

A

S

D

A

S

Q

R

L

L

I

I

V

A

R

A

K

K

D

G

E

S

K

P

R

I

E

Q

F

-

K

P

S

T

L

L

E

E

D

S

L

L

K

K

G

G

K

K

K

H

L

V

G

G

L

V

G
x
L

Q

Q

G

G

T
x
S

N

T

F
x
Q

E

E

Q

A

K

Y

A

A

K

N

-

D

-

N

-

W

A

R

V

T

P

K

G

G

I

V

D

D

V

V

R

V

T

A

Y

Y

P

A

G

N

S

Q

P

D

E

L

Y

I

L

Y

A

S

D

D

L

L

A

T

A

A

G

G

R

R

I

L

D

D

A

A

L

L

N

Q

D
|
D

S

E

L

V

L

A

V

A

-

S

-

E

G

G

Y

F

I

L

L

K

K

Q

S

P

T

A

N

G

L

K

P

E

L

Y

K

A

A

F

G

A

S

G

P

P

-

S

-

V

-

K

-

D

-

K

-

Y

I

F

G

G

A

-

V

-

D

D

K

G

I

T

G

G

I

V

P

G

F

L

R

R

K

K

G

D

N

D

P

T

E

E

F

L

K

K

A

A

L

F

N

D

K

K

A

A

L

L

A

T

D

E

I

L

K

R

A

Q

D

D

G

G

S

T

F

Y

K

D

A

K

A

M

S

A

E

K

K

K

W

Y

F

F

G

D

I

F

D

N

V

V

binding histidine: Y14 (= Y47), F52 (≠ W86), S69 (≠ N103), S70 (≠ Q104), L71 (≠ V105), S72 (≠ G106), R77 (= R111), L117 (≠ G152), S120 (≠ T155), Q122 (≠ F157), D161 (= D193)

1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
32% identity, 84% coverage: 38:260/267 of query aligns to 5:235/238 of 1lafE

query
sites
1lafE

T

T

L

V

K

R

V

I

A

G

L

T

E
x
D

G

T

N

T

Y
|
Y

P

A

P

P

F

F

N

S

F

S

K

K

D

D

P

A

K

K

T

-

G

G

E

E

L

F

T

I

G

G

F

F

E

D

V

I

D

D

V

L

A

G

K

N

L

E

L

M

A

C

A

K

K

R

L

M

G

Q

V

V

K

K

P

C

V

T

F

W

T

V

T

A

T

S

E

D

W
x
F

S

D

G

A

I

L

L

I

A

P

G

S

L

L

G

K

A

A

G

K

K

K

Y

I

D

D

V

A

I

I

N
x
S

Q
x
S

V

L

G
x
S

I

I

T

T

D

D

E

K

R
|
R

Q

Q

K

Q

A

E

F

I

D

A

F

F

S

S

D

D

P

K

Y

L

T

Y

L

A

S

A

S

D

A

S

Q

R

L

L

I

I

V

A

R

A

K

K

D

G

E

S

K

P

R

I

E

Q

F

-

K

P

S

T

L

L

E

E

D

S

L

L

K

K

G

G

K

K

K

H

L

V

G

G

L

V

G
x
L

Q

Q

G

G

T
x
S

N
x
T

F
x
Q

E

E

Q

A

K

Y

A

A

K

N

-

D

-

N

-

W

A

R

V

T

P

K

G

G

I

V

D

D

V

V

R

V

T

A

Y

Y

P

A

G

N

S

Q

P

D

E

L

Y

I

L

Y

A

S

D

D

L

L

A

T

A

A

G

G

R

R

I

L

D

D

A

A

L

L

N

Q

D
|
D

S

E

L

V

L

A

V

A

-

S

-

E

G

G

Y

F

I

L

L

K

K

Q

S

P

T

A

N

G

L

K

P

E

L

Y

K

A

A

F

G

A

S

G

P

P

-

S

-

V

-

K

-

D

-

K

-

Y

I

F

G

G

A

-

V

-

D

D

K

G

I

T

G

G

I

V

P

G

F

L

R

R

K

K

G

D

N

D

P

T

E

E

F

L

K

K

A

A

L

F

N

D

K

K

A

A

L

L

A

T

D

E

I

L

K

R

A

Q

D

D

G

G

S

T

F

Y

K

D

A

K

A

M

S

A

E

K

K

K

W

Y

F

F

G

D

I

F

D

N

V

V

binding arginine: D11 (≠ E44), Y14 (= Y47), F52 (≠ W86), S69 (≠ N103), S70 (≠ Q104), S72 (≠ G106), R77 (= R111), L117 (≠ G152), S120 (≠ T155), T121 (≠ N156), Q122 (≠ F157), D161 (= D193)

5otaA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with octopinic acid (see paper)
32% identity, 84% coverage: 38:261/267 of query aligns to 3:253/254 of 5otaA

query
sites
5otaA

T

S

L

I

K

T

V

I

A

A

L

T

E
|
E

G

G

N

S

Y
|
Y

P

A

P

P

F

Y

N

N

F

F

K

K

D

D

P

-

K

A

T

G

G

G

E

K

L

L

T

I

G

G

F

F

E

D

V

I

D

D

V

L

A

G

K

N

L

D

L

L

A

C

A

K

K

R

L

M

G

N

V

I

K

E

P

C

V

K

F

F

T

V

T

E

T

Q

E

A

W
|
W

S

D

G

G

I

I

L

I

A

P

G

S

L

L

G

T

A

A

G

G

K

R

Y

Y

D

D

V

A

I

I

I

M

N

A

Q
x
A

V

M

G
|
G

I

I

T

Q

D

P

E

A

R
|
R

Q

E

K

K

A

V

F

I

D

A

F

F

S

S

D

R

P

P

Y

Y

T

L

L

L

S

T

S

P

A
x
M

Q

T

L

F

I

L

V

T

R

T

K

A

D

D

-

S

-

P

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

-

N

-

L

-

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

E

Q

K

K

R

A

E

E

F

L

K

D

S

K

L

F

E

T

D

K

L

I

-

F

K

E

G

G

K

V

K

K

L

F

G

G

L

V

G
x
Q

Q

A

G

G

T
|
T

N
x
S

F
x
H

E

E

Q

A

K

F

A

M

K

K

-

Q

A

M

V

M

P

P

G

S

I

V

D

Q

V

I

R

S

T

T

Y

Y

P

D

G

T

S

I

P

D

E

N

Y

V

L

V

A

M

D

D

L

L

A

K

A

A

G

G

R

R

I

I

D

D

A

A

A

S

L

L

N

A

D
x
S

-

V

S

S

L

F

L

L

V

K

G

P

Y

L

I

T

L

D

K

K

S

P

T

D

N

N

L

K

P

D

L

L

K

K

A

M

G

F

S

G

P

P

I

R

-

M

-

T

-

G

G

G

A

L

V

F

D

G

K

K

-

G

I

V

G

G

I

V

P

G

F

I

R

R

K

K

G

E

N

D

P

A

E

D

F

L

K

K

A

A

A

L

L

F

N

D

K

K

A

A

L

I

A

D

D

A

I

A

K

I

A

A

D

D

G

G

S

T

F

V

K

Q

A

K

A

L

S

S

E

Q

K

Q

W

W

F

F

G

G

I

Y

D

D

V

A

S

S

binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: E9 (= E44), Y12 (= Y47), W50 (= W86), A68 (≠ Q104), G70 (= G106), R75 (= R111), M90 (≠ A126), Q138 (≠ G152), T141 (= T155), S142 (≠ N156), H143 (≠ F157), S180 (≠ D193)

5ot9A Structure of the periplasmic binding protein (pbp) noct from a.Tumefaciens c58 in complex with histopine. (see paper)
32% identity, 84% coverage: 38:261/267 of query aligns to 3:253/254 of 5ot9A

query
sites
5ot9A

T

S

L

I

K

T

V

I

A

A

L

T

E
|
E

G

G

N

S

Y
|
Y

P

A

P

P

F

Y

N

N

F

F

K

K

D

D

P

-

K

A

T

G

G

G

E

K

L

L

T

I

G

G

F

F

E

D

V

I

D

D

V

L

A

G

K

N

L

D

L

L

A

C

A

K

K

R

L

M

G

N

V

I

K

E

P

C

V

K

F

F

T

V

T

E

T

Q

E

A

W
|
W

S

D

G

G

I

I

L

I

A

P

G

S

L

L

G

T

A

A

G

G

K

R

Y

Y

D

D

V

A

I

I

I

M

N
x
A

Q
x
A

V

M

G

G

I

I

T

Q

D

P

E

A

R
|
R

Q

E

K

K

A

V

F

I

D

A

F

F

S

S

D

R

P

P

Y

Y

T

L

L

L

S

T

S

P

A
x
M

Q

T

L

F

I

L

V

T

R

T

K

A

D

D

-

S

-

P

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

-

N

-

L

-

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

E

Q

K

K

R

A

E

E

F

L

K

D

S

K

L

F

E

T

D

K

L

I

-

F

K

E

G

G

K

V

K

K

L

F

G

G

L

V

G
x
Q

Q

A

G

G

T
|
T

N
x
S

F
x
H

E

E

Q

A

K

F

A

M

K

K

-

Q

A

M

V

M

P

P

G

S

I

V

D

Q

V

I

R

S

T

T

Y

Y

P

D

G

T

S

I

P

D

E

N

Y

V

L

V

A

M

D

D

L

L

A

K

A

A

G

G

R

R

I

I

D

D

A

A

A

S

L

L

N

A

D
x
S

-

V

S

S

L

F

L

L

V

K

G

P

Y

L

I

T

L

D

K

K

S

P

T

D

N

N

L

K

P

D

L

L

K

K

A

M

G

F

S

G

P

P

I

R

-

M

-

T

-

G

G

G

A

L

V

F

D

G

K

K

-

G

I

V

G

G

I

V

P

G

F

I

R

R

K

K

G

E

N

D

P

A

E

D

F

L

K

K

A

A

A

L

L

F

N

D

K

K

A

A

L

I

A

D

D

A

I

A

K

I

A

A

D

D

G

G

S

T

F

V

K

Q

A

K

A

L

S

S

E

Q

K

Q

W

W

F

F

G

G

I

Y

D

D

V

A

S

S

binding Histopine: E9 (= E44), Y12 (= Y47), W50 (= W86), A67 (≠ N103), A68 (≠ Q104), R75 (= R111), M90 (≠ A126), Q138 (≠ G152), T141 (= T155), S142 (≠ N156), H143 (≠ F157), S180 (≠ D193)

Query Sequence

>HSERO_RS10775 HSERO_RS10775 cysteine ABC transporter substrate-binding protein
MNKNIKQWLLAGVGAALLASSLSVSAADLLQSVKSSGTLKVALEGNYPPFNFKDPKTGEL
TGFEVDVAKLLAAKLGVKPVFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDP
YTLSSAQLIVRKDEKREFKSLEDLKGKKLGLGQGTNFEQKAKAVPGIDVRTYPGSPEYLA
DLAAGRIDAALNDSLLVGYILKSTNLPLKAGSPIGAVDKIGIPFRKGNPEFKAALNKALA
DIKADGSFKAASEKWFGIDVSQPPKAQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory