SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS11450 HSERO_RS11450 ABC transporter ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
60% identity, 96% coverage: 4:249/257 of query aligns to 1:240/241 of 4u00A

query
sites
4u00A

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binding adenosine-5'-diphosphate: F12 (= F15), L15 (≠ H18), V17 (= V20), S37 (= S40), G38 (= G41), S39 (= S42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

3c4jA Abc protein artp in complex with atp-gamma-s
58% identity, 95% coverage: 5:249/257 of query aligns to 3:242/242 of 3c4jA

query
sites
3c4jA

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binding phosphothiophosphoric acid-adenylate ester: F13 (= F15), V18 (= V20), P37 (= P39), S38 (= S40), G39 (= G41), S40 (= S42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46), H133 (≠ K140), Y135 (= Y142), E164 (= E171)

3c41J Abc protein artp in complex with amp-pnp/mg2+
58% identity, 95% coverage: 5:249/257 of query aligns to 3:242/242 of 3c41J

query
sites
3c41J

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binding phosphoaminophosphonic acid-adenylate ester: F13 (= F15), V18 (= V20), P37 (= P39), S38 (= S40), G39 (= G41), S40 (= S42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46), R47 (= R49), H133 (≠ K140), Y135 (= Y142)
binding magnesium ion: S43 (= S45), D163 (= D170)

2olkA Abc protein artp in complex with adp-beta-s
58% identity, 95% coverage: 5:249/257 of query aligns to 3:242/242 of 2olkA

query
sites
2olkA

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binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F15), L16 (≠ H18), V18 (= V20), S38 (= S40), G39 (= G41), S40 (= S42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

2oljA Abc protein artp in complex with adp/mg2+
58% identity, 95% coverage: 5:249/257 of query aligns to 3:242/242 of 2oljA

query
sites
2oljA

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E

E

A

E

G

G

P

K

P

P

G

E

A

D

I

L

F

F

T

D

A

R

P

P

Q

Q

Q

H

A

E

R

R

T

T

R

K

S

A

F

F

L

L

Q

S

T

K

M

V

L

F

binding adenosine-5'-diphosphate: F13 (= F15), V18 (= V20), S38 (= S40), G39 (= G41), S40 (= S42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46), H133 (≠ K140), Y135 (= Y142)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
57% identity, 94% coverage: 8:249/257 of query aligns to 4:240/240 of 4ymuJ

query
sites
4ymuJ

V

V

S

N

G

D

L

V

R

Y

K

K

S

N

F
|
F

G

G

A

S

H
x
L

V

E

V
|
V

L

L

K

K

D

G

L

V

D

T

L

L

S

K

V

V

A

N

P

K

S

G

Q

E

V

V

V

V

V

V

V

I

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

C

L

I

N

N

G

L

L

L

E
|
E

K

E

A

P

E

T

G

K

G

G

T

E

V

V

K

F

V

I

C

D

G

G

R

V

P

K

V

I

V

N

E

N

G

G

G

-

R

-

M

-

M

-

P

-

E

K

A

V

M

N

L

I

D

N

L

K

L

V

R

R

A

Q

E

K

V

V

G

G

M

M

V

V

F

F

Q

Q

S

H

F

F

N

N

L

L

F

F

P

P

H

H

L

L

T

T

V

A

L

I

D

E

N

N

I

I

T

T

L

L

A

A

P

P

T

V

C

K

L

V

R

K

G

K

M

M

S

N

R

K

K

K

A

E

A

A

Q

E

Q

E

Q

L

A

A

L

V

Q

D

L

L

L

L

E

A

K

K

V

V

G

G

L

L

A

L

H

D

K

K

A

K

K

D

A

Q

Y

Y

P

P

G

I

T

K

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

L

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

E

Q

P

P

Q

E

V

V

M

M

L

L

F

F

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

M

V

V

G

K

E

E

V

V

L

L

Q

N

V

V

I

M

R

K

A

Q

L

L

A

A

A

N

E

E

G

G

M

M

T

T

M

M

I

V

I

V

V

V

T

T

H
|
H

E

E

M

M

G

G

F

F

A

A

R

R

E

E

V

V

A

G

D

D

V

R

V

V

V

I

V

F

M

M

D

D

H

D

G

G

S

V

I

I

V

V

E

E

A

E

G

G

P

T

P

P

G

E

A

E

I

I

F

F

T

Y

A

R

P

A

Q

K

Q

N

A

E

R

R

T

T

R

R

S

E

F

F

L

L

Q

S

T

K

M

I

L

L

binding adenosine-5'-triphosphate: F11 (= F15), L14 (≠ H18), V16 (= V20), S36 (= S40), G37 (= G41), S38 (= S42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46), E51 (= E55), E162 (= E171), H194 (= H203)
binding magnesium ion: S41 (= S45), E162 (= E171)

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
50% identity, 96% coverage: 6:251/257 of query aligns to 3:255/258 of 1b0uA

query
sites
1b0uA

V

L

E

H

V

V

S

I

G

D

L

L

R

H

K

K

S

R

F
x
Y

G

G

A

G

H
|
H

V

E

V
|
V

L

L

K

K

D

G

L

V

D

S

L

L

S

Q

V

A

A

R

P

A

S

G

Q

D

V

V

V

I

V

S

V

I

I

I

G

G

P

S

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

L

I

N

N

G

F

L

L

E

E

K

K

A

P

E

S

G

E

G

G

T

A

V

I

K

I

V

V

C

N

G

G

R

Q

P

N

V

I

V

N

-

L

-

V

-

R

-

D

E

K

G

D

G

G

R

Q

M

L

M

K

-

V

-

A

P

D

E

K

A

N

M

Q

L

L

D

R

L

L

L

L

R

R

A

T

E

R

V

L

G

T

M

M

V

V

F

F

Q

Q

S

H

F

F

N

N

L

L

F

W

P

S

H

H

L

M

T

T

V

V

L

L

D

E

N

N

I

V

T

M

L

E

A

A

P

P

T

I

C

Q

L

V

R

L

G

G

M

L

S

S

R

K

K

H

A

D

A

A

Q

R

Q

E

Q

R

A

A

L

L

Q

K

L

Y

L

L

E

A

K

K

V

V

G

G

L

I

A

D

H

E

K

R

A

A

K

Q

A

G

-

K

Y

Y

P

P

G

V

T

H

L

L

S

S

G

G

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

A

M

M

E

E

P

P

Q

D

V

V

M

L

L

L

F

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

L

V

V

G

G

E

E

V

V

L

L

Q

R

V

I

I

M

R

Q

A

Q

L

L

A

A

A

E

E

E

G

G

M

K

T

T

M

M

I

V

I

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

E

H

V

V

A

S

D

S

V

H

V

V

V

I

V

F

M

L

D

H

H

Q

G

G

S

K

I

I

V

E

E

E

A

E

G

G

P

D

P

P

G

E

A

Q

I

V

F

F

T

G

A

N

P

P

Q

Q

Q

S

A

P

R

R

T

L

R

Q

S

Q

F

F

L

L

Q

K

T

G

M

S

L

L

A

K

R

K

binding adenosine-5'-triphosphate: Y12 (≠ F15), H15 (= H18), V17 (= V20), S37 (= S40), G38 (= G41), S39 (= S42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 95% coverage: 5:249/257 of query aligns to 1:245/343 of P30750

query
sites
P30750

I

M

V

I

E

K

V

L

S

S

G

N

L

I

R

T

K

K

S

V

F

F

-

H

-

Q

G

G

A

T

H

R

V

T

V

I

-

Q

-

A

L

L

K

N

D

N

L

V

D

S

L

L

S

H

V

V

A

P

P

A

S

G

Q

Q

V

I

V

Y

V

G

V

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

L

V

N

N

G

L

L

L

E

E

K

R

A

P

E

T

G

E

G

G

T

S

V

V

K

L

V

V

C

D

G

G

R

Q

P

E

V

L

V

T

E

-

G

-

G

-

R

-

M

T

M

L

P

S

E

E

A

S

M

E

L

L

D

T

L

K

L

A

R

R

A

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

S

H

F

F

N

N

L

L

F

L

P

S

H

S

L

R

T

T

V

V

L

F

D

G

N

N

I

V

T

A

L

L

A

-

P

P

T

L

C

E

L

L

R

D

G

N

M

T

S

P

R

K

K

D

A

E

A

V

Q

K

Q

R

Q

R

A

V

L

T

Q

E

L

L

L

L

E

S

K

L

V

V

G

G

L

L

A

G

H

D

K

K

A

H

K

D

A

S

Y

Y

P

P

G

S

T

N

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

E

N

P

P

Q

K

V

V

M

L

L

L

F

C

D

D

E
|
E

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

Q

E

V

L

I

L

R

K

A

D

L

I

A

N

A

R

E

R

-

L

G

G

M

L

T

T

M

I

I

L

I

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

R

K

E

R

V

I

A

C

D

D

V

C

V

V

V

A

V

V

M

I

D

S

H

N

G

G

S

E

I

L

V

I

E

E

A

Q

G

D

P

T

P

V

G

S

A

E

I

V

F

F

T

S

A

H

P

P

Q

K

Q

T

A

P

R

L

T

A

R

Q

S

K

F

F

L

I

Q

Q

T

S

M

T

L

L

40:46 (vs. 40:46, 86% identical) binding
E166 (= E171) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
37% identity, 95% coverage: 5:249/257 of query aligns to 2:246/344 of 6cvlD

query
sites
6cvlD

I

M

V

I

E

K

V

L

S

S

G

N

L

I

R

T

K

K

S

V

F
|
F

-

H

-
x
Q

G

G

A

T

H

R

V

T

V
x
I

-

Q

-

A

L

L

K

N

D

N

L

V

D

S

L

L

S

H

V

V

A

P

P

A

S

G

Q

Q

V

I

V

Y

V

G

V

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

L

V

N

N

G

L

L

L

E

E

K

R

A

P

E

T

G

E

G

G

T

S

V

V

K

L

V

V

C

D

G

G

R

Q

P

E

V

L

V

T

E

-

G

-

G

-

R

-

M

T

M

L

P

S

E

E

A

S

M

E

L

L

D

T

L

K

L

A

R

R

A

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

S

H

F

F

N

N

L

L

F

L

P

S

H

S

L

R

T

T

V

V

L

F

D

G

N

N

I

V

T

A

L

L

A

-

P

P

T

L

C

E

L

L

R

D

G

N

M

T

S

P

R

K

K

D

A

E

A

V

Q

K

Q

R

Q

R

A

V

L

T

Q

E

L

L

L

L

E

S

K

L

V

V

G

G

L

L

A

G

H

D

K

K

A

H

K

D

A

S

Y

Y

P

P

G

S

T
x
N

L
|
L

S
|
S

G
|
G

G
|
G

Q
|
Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

E

N

P

P

Q

K

V

V

M

L

L

L

F

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

Q

E

V

L

I

L

R

K

A

D

L

I

A

N

A

R

E

R

-

L

G

G

M

L

T

T

M

I

I

L

I

L

V

I

T

T

H
|
H

E

E

M

M

G

D

F

V

A

V

R

K

E

R

V

I

A

C

D

D

V

C

V

V

V

A

V

V

M

I

D

S

H

N

G

G

S

E

I

L

V

I

E

E

A

Q

G

D

P

T

P

V

G

S

A

E

I

V

F

F

T

S

A

H

P

P

Q

K

Q

T

A

P

R

L

T

A

R

Q

S

K

F

F

L

I

Q

Q

T

S

M

T

L

L

binding phosphothiophosphoric acid-adenylate ester: F12 (= F15), Q14 (vs. gap), I19 (≠ V20), S41 (= S40), G42 (= G41), A43 (≠ S42), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46), N141 (≠ T145), L142 (= L146), S143 (= S147), G144 (= G148), G145 (= G149), Q146 (= Q150), H200 (= H203)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
37% identity, 95% coverage: 5:249/257 of query aligns to 2:246/344 of 3tuzC

query
sites
3tuzC

I

M

V

I

E

K

V

L

S

S

G

N

L

I

R

T

K

K

S

V

F
|
F

-

H

-
x
Q

G

G

A

T

H

R

V

T

V

I

-

Q

-
x
A

L

L

K

N

D

N

L

V

D

S

L

L

S

H

V

V

A

P

P

A

S

G

Q

Q

V

I

V

Y

V

G

V

V

I

I

G

G

P

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

L

V

N

N

G

L

L

L

E

E

K

R

A

P

E

T

G

E

G

G

T

S

V

V

K

L

V

V

C

D

G

G

R

Q

P

E

V

L

V

T

E

-

G

-

G

-

R

-

M

T

M

L

P

S

E

E

A

S

M

E

L

L

D

T

L

K

L

A

R

R

A

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

S

H

F

F

N

N

L

L

F

L

P

S

H

S

L

R

T

T

V

V

L

F

D

G

N

N

I

V

T

A

L

L

A

-

P

P

T

L

C

E

L

L

R

D

G

N

M

T

S

P

R

K

K

D

A

E

A

V

Q

K

Q

R

Q

R

A

V

L

T

Q

E

L

L

L

L

E

S

K

L

V

V

G

G

L

L

A

G

H

D

K

K

A

H

K

D

A

S

Y

Y

P

P

G

S

T

N

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

E

N

P

P

Q

K

V

V

M

L

L

L

F

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

Q

E

V

L

I

L

R

K

A

D

L

I

A

N

A

R

E

R

-

L

G

G

M

L

T

T

M

I

I

L

I

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

R

K

E

R

V

I

A

C

D

D

V

C

V

V

V

A

V

V

M

I

D

S

H

N

G

G

S

E

I

L

V

I

E

E

A

Q

G

D

P

T

P

V

G

S

A

E

I

V

F

F

T

S

A

H

P

P

Q

K

Q

T

A

P

R

L

T

A

R

Q

S

K

F

F

L

I

Q

Q

T

S

M

T

L

L

binding adenosine-5'-diphosphate: F12 (= F15), Q14 (vs. gap), A21 (vs. gap), G42 (= G41), A43 (≠ S42), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 312, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
37% identity, 95% coverage: 5:249/257 of query aligns to 2:246/344 of 3tuiC

query
sites
3tuiC

I

M

V

I

E

K

V

L

S

S

G

N

L

I

R

T

K

K

S

V

F
|
F

-

H

-
x
Q

G

G

A

T

H

R

V

T

V

I

-

Q

-

A

L

L

K

N

D

N

L

V

D

S

L

L

S

H

V

V

A

P

P

A

S

G

Q

Q

V

I

V

Y

V

G

V

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

L

V

N

N

G

L

L

L

E

E

K

R

A

P

E

T

G

E

G

G

T

S

V

V

K

L

V

V

C

D

G

G

R

Q

P

E

V

L

V

T

E

-

G

-

G

-

R

-

M

T

M

L

P

S

E

E

A

S

M

E

L

L

D

T

L

K

L

A

R

R

A

R

E

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

S

H

F

F

N

N

L

L

F

L

P

S

H

S

L

R

T

T

V

V

L

F

D

G

N

N

I

V

T

A

L

L

A

-

P

P

T

L

C

E

L

L

R

D

G

N

M

T

S

P

R

K

K

D

A

E

A

V

Q

K

Q

R

Q

R

A

V

L

T

Q

E

L

L

L

L

E

S

K

L

V

V

G

G

L

L

A

G

H

D

K

K

A

H

K

D

A

S

Y

Y

P

P

G

S

T

N

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

E

N

P

P

Q

K

V

V

M

L

L

L

F

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

Q

E

V

L

I

L

R

K

A

D

L

I

A

N

A

R

E

R

-

L

G

G

M

L

T

T

M

I

I

L

I

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

R

K

E

R

V

I

A

C

D

D

V

C

V

V

V

A

V

V

M

I

D

S

H

N

G

G

S

E

I

L

V

I

E

E

A

Q

G

D

P

T

P

V

G

S

A

E

I

V

F

F

T

S

A

H

P

P

Q

K

Q

T

A

P

R

L

T

A

R

Q

S

K

F

F

L

I

Q

Q

T

S

M

T

L

L

binding adenosine-5'-diphosphate: F12 (= F15), Q14 (vs. gap), S41 (= S40), G42 (= G41), A43 (≠ S42), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
36% identity, 93% coverage: 5:243/257 of query aligns to 3:231/592 of 5lj7A

query
sites
5lj7A

I

I

V

I

E

E

V

I

S

K

G

Q

L

L

R

N

K

R

S

Y

F
|
F

G

G

-

E

-

G

-

E

-

N

-

R

A

V

H

H

V
|
V

V

-

L

L

K

K

D

D

L

I

D

S

L

L

S

S

V

I

A

E

P

R

S

G

Q

D

V

F

V

V

V

A

V

I

I

M

G

G

P

Q

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

M

R

N

C

I

L

I

N

G

G

C

L

L

E

D

K

T

A

A

E

T

G

G

G

G

T

S

V

S

K

K

V

I

C

D

G

G

R

K

P

E

V

T

V

I

E

E

G

-

G

-

R

-

M

L

M

T

P

N

E

D

A

Q

M

L

L

S

D

D

L

L

L

R

R

S

A

Q

E

K

V

F

G

G

M

F

V

I

F

F

Q
|
Q

S

R

F

Y

N

N

L

L

F

L

P

S

H

S

L

L

T

T

V

A

L

A

D

E

N

N

I

V

T

A

L

L

A

-

P

P

T

A

C

I

L

Y

R

A

G

G

M

M

S

P

R

Q

K

S

A

Q

A

R

Q

L

Q

E

Q

R

A

A

L

K

Q

Q

L

L

L

L

E

E

K

K

V

L

G

G

L

L

A

G

H

D

K
|
K

A

W

K

Q

A

N

Y

K

P

P

G
x
N

T
x
Q

L
|
L

S
|
S

G
|
G

G
|
G

Q
|
Q

K

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

M

N

E

G

P

G

Q

E

V

I

M

I

L

L

F

A

D
|
D

E
x
Q

P

P

T

T

S

G

A
|
A

L

L

D

D

P

S

E

H

L

S

V

G

G

E

E

N

V

V

L

M

Q

E

V

I

I

L

R

R

A

Q

L

L

A

H

A

E

E

E

G

G

M

H

T

T

M

I

I

I

I

M

V

V

T

T

H
|
H

E

D

M

K

G

H

F

I

A

A

R

A

E

S

V

A

A

N

D

R

V

I

V

I

V

E

V

I

M

K

D

D

H

-

G

-

S

-

I

-

V

-

E

-

A

-

G

-

P

-

P

-

G

G

A

E

I

I

F

I

T

S

A

D

P

T

Q

Q

Q

K

A

R

R

Q

T

V

R

K

S

S

binding adenosine-5'-triphosphate: F13 (= F15), V22 (= V19), S42 (= S40), G43 (= G41), S44 (= S42), G45 (= G43), K46 (= K44), S47 (= S45), T48 (= T46), Q92 (= Q93), K136 (= K138), N142 (≠ G144), Q143 (≠ T145), L144 (= L146), S145 (= S147), G146 (= G148), G147 (= G149), Q148 (= Q150), Q169 (≠ E171), A173 (= A175), H201 (= H203)
binding magnesium ion: S47 (= S45), Q92 (= Q93), D168 (= D170)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
36% identity, 93% coverage: 5:243/257 of query aligns to 3:231/615 of 5lilA

query
sites
5lilA

I

I

V

I

E

E

V

I

S

K

G

Q

L

L

R

N

K

R

S

Y

F
|
F

G

G

-

E

-

G

-

E

-

N

-

R

A

V

H

H

V
|
V

V

-

L

L

K

K

D

D

L

I

D

S

L

L

S

S

V

I

A

E

P

R

S

G

Q

D

V

F

V

V

V

A

V

I

I

M

G

G

P

Q

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

M

R

N

C

I

L

I

N

G

G

C

L

L

E

D

K

T

A

A

E

T

G

G

G

G

T

S

V

S

K

K

V

I

C

D

G

G

R

K

P

E

V

T

V

I

E

E

G

-

G

-

R

-

M

L

M

T

P

N

E

D

A

Q

M

L

L

S

D

D

L

L

L

R

R

S

A

Q

E

K

V

F

G

G

M

F

V

I

F

F

Q
|
Q

S

R

F

Y

N

N

L

L

F

L

P

S

H

S

L

L

T

T

V

A

L

A

D

E

N

N

I

V

T

A

L

L

A

-

P

P

T

A

C

I

L

Y

R

A

G

G

M

M

S

P

R

Q

K

S

A

Q

A

R

Q

L

Q

E

Q

R

A

A

L

K

Q

Q

L

L

L

L

E

E

K

K

V

L

G

G

L

L

A

G

H

D

K
|
K

A

W

K

Q

A
x
N

Y

K

P

P

G

N

T
x
Q

L
|
L

S
|
S

G
|
G

G
|
G

Q
|
Q

K

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

M

N

E

G

P

G

Q

E

V

I

M

I

L

L

F

A

D
|
D

E
x
Q

P

P

T

T

S

G

A
|
A

L

L

D

D

P

S

E

H

L

S

V

G

G

E

E

N

V

V

L

M

Q

E

V

I

I

L

R

R

A

Q

L

L

A

H

A

E

E

E

G

G

M

H

T

T

M

I

I

I

I

M

V

V

T

T

H
|
H

E

D

M

K

G

H

F

I

A

A

R

A

E

S

V

A

A

N

D

R

V

I

V

I

V

E

V

I

M

K

D

D

H

-

G

-

S

-

I

-

V

-

E

-

A

-

G

-

P

-

P

-

G

G

A

E

I

I

F

I

T

S

A

D

P

T

Q

Q

Q

K

A

R

R

Q

T

V

R

K

S

S

binding adenosine-5'-triphosphate: F13 (= F15), V22 (= V19), S42 (= S40), G43 (= G41), S44 (= S42), G45 (= G43), K46 (= K44), S47 (= S45), T48 (= T46), Q92 (= Q93), K136 (= K138), N139 (≠ A141), Q143 (≠ T145), L144 (= L146), S145 (= S147), G146 (= G148), G147 (= G149), Q148 (= Q150), Q169 (≠ E171), A173 (= A175), H201 (= H203)
binding magnesium ion: S47 (= S45), Q92 (= Q93), D168 (= D170)

6z4wA Ftse structure from streptococcus pneumoniae in complex with adp (space group p 1) (see paper)
33% identity, 88% coverage: 5:230/257 of query aligns to 3:224/230 of 6z4wA

query
sites
6z4wA

I

I

V

I

E

E

V

M

S

R

G

D

L

V

R

V

K

K

S

K

F
x
Y

-

D

G
x
N

A

G

H
x
T

V

T

V
x
A

L

L

K

R

D

G

L

V

D

S

L

V

S

S

V

V

A

Q

P

P

S

G

Q

E

V

F

V

A

V

Y

V

I

I

V

G

G

P

P

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

I

R

R

C

S

L

L

N

Y

G

R

L

E

E

V

K

K

A

I

E

D

G

K

G

G

T

S

V

L

K

S

V

V

C

A

G

G

R

F

P

N

V

L

V

V

E

K

G

-

G

-

R

-

M

-

M

I

P

K

E

K

A

K

M

D

L

V

D

P

L

L

L

L

R

R

A

R

E

S

V

V

G

G

M

V

V

V

F

F

Q

Q

S

D

F

Y

N

K

L

L

F

L

P

P

H

K

L

K

T

T

V

V

L

Y

D

E

N

N

I

I

T

A

L

Y

A

A

P

M

T

E

C

V

L

I

R

-

G

G

M

E

S

N

R

R

K

R

A

N

A

I

Q

K

Q

R

Q

R

A

V

L

M

Q

E

L

V

L

L

E

D

K

L

V

V

G

G

L

L

A

K

H

H

K

K

A

V

K

R

A

S

Y

F

P

P

G

N

T

E

L

L

S

S

G

G

G

G

Q

E

K

Q

Q

Q

R

R

V

I

A

A

I

I

A

A

R

R

A

A

L

I

A

V

M

N

E

N

P

P

Q

K

V

V

M

L

L

I

F

A

D

D

E

E

P

P

T

T

S

G

A

N

L

L

D

D

P

P

E

D

L

N

V

S

G

W

E

E

V

I

L

M

Q

N

V

L

I

L

R

E

A

R

L

I

A

N

A

L

E

Q

G

G

M

T

T

T

M

I

I

L

I

M

V

A

T

T

H

H

E

N

M

S

G

Q

F

I

A

V

R

N

E

T

V

L

A

R

D

H

V

R

V

V

V

I

V

A

M

I

D

E

H

N

G

G

S

R

I

V

V

V

E

R

A

D

G

E

P

S

P

K

G

G

binding adenosine-5'-diphosphate: Y13 (≠ F15), N15 (≠ G16), T17 (≠ H18), A19 (≠ V20), S39 (= S40), G40 (= G41), A41 (≠ S42), G42 (= G43), K43 (= K44), S44 (= S45), T45 (= T46)

6z67B Ftse structure of streptococcus pneumoniae in complex with amppnp at 2.4 a resolution (see paper)
33% identity, 88% coverage: 5:230/257 of query aligns to 3:224/229 of 6z67B

query
sites
6z67B

I

I

V

I

E

E

V

M

S

R

G

D

L

V

R

V

K

K

S

K

F
x
Y

-

D

G

N

A
x
G

H
x
T

V

T

V
x
A

L

L

K

R

D

G

L

V

D

S

L

V

S

S

V

V

A

Q

P

P

S

G

Q

E

V

F

V

A

V

Y

V

I

I

V

G

G

P

P

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

I

R

R

C

S

L

L

N

Y

G

R

L

E

E

V

K

K

A

I

E

D

G

K

G

G

T

S

V

L

K

S

V

V

C

A

G

G

R

F

P

N

V

L

V

V

E

K

G

-

G

-

R

-

M

-

M

I

P

K

E

K

A

K

M

D

L

V

D

P

L

L

L

L

R

R

A

R

E

S

V

V

G

G

M

V

V

V

F

F

Q

Q

S

D

F

Y

N

K

L

L

F

L

P

P

H

K

L

K

T

T

V

V

L

Y

D

E

N

N

I

I

T

A

L

Y

A

A

P

M

T

E

C

V

L

I

R

-

G

G

M

E

S

N

R

R

K

R

A

N

A

I

Q

K

Q

R

Q

R

A

V

L

M

Q

E

L

V

L

L

E

D

K

L

V

V

G

G

L

L

A

K

H

H

K

K

A

V

K

R

A

S

Y

F

P

P

G

N

T

E

L

L

S

S

G

G

G

G

Q

E

K

Q

Q

Q

R

R

V

I

A

A

I

I

A

A

R

R

A

A

L

I

A

V

M

N

E

N

P

P

Q

K

V

V

M

L

L

I

F

A

D

D

E

E

P

P

T

T

S

G

A

N

L

L

D

D

P

P

E

D

L

N

V

S

G

W

E

E

V

I

L

M

Q

N

V

L

I

L

R

E

A

R

L

I

A

N

A

L

E

Q

G

G

M

T

T

T

M

I

I

L

I

M

V

A

T

T

H
|
H

E

N

M

S

G

Q

F

I

A

V

R

N

E

T

V

L

A

R

D

H

V

R

V

V

V

I

V

A

M

I

D

E

H

N

G

G

S

R

I

V

V

V

E

R

A

D

G

E

P

S

P

K

G

G

binding phosphoaminophosphonic acid-adenylate ester: Y13 (≠ F15), G16 (≠ A17), T17 (≠ H18), A19 (≠ V20), S39 (= S40), G40 (= G41), A41 (≠ S42), G42 (= G43), K43 (= K44), S44 (= S45), T45 (= T46), H197 (= H203)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
39% identity, 88% coverage: 4:229/257 of query aligns to 3:226/648 of P75831

query
sites
P75831

P

P

I

L

V

L

E

E

V

L

S

K

G

D

L

I

R

R

K

R

S

S

F

Y

G

P

A

A

H

G

-

D

-

E

-

Q

-

V

V

E

V

V

L

L

K

K

D

G

L

I

D

S

L

L

S

D

V

I

A

Y

P

A

S

G

Q

E

V

M

V

V

V

A

V

I

I

V

G

G

P

A

S

S

G

G

S

S

G

G

K
|
K

S

S

T

T

F

L

L

M

R

N

C

I

L

L

N

G

G

C

L

L

E

D

K

K

A

A

E

T

G

S

G

G

T

T

V

Y

K

R

V

V

C

A

G

G

R

Q

P

D

V

V

V

A

E

T

G

-

G

-

R

-

M

L

M

D

P

A

E

D

A

A

M

L

L

A

D

Q

L

L

L

R

R

R

A

E

E

H

V

F

G

G

M

F

V

I

F

F

Q

Q

S

R

F

Y

N

H

L

L

F

L

P

S

H

H

L

L

T

T

V

A

L

E

D

Q

N

N

I

V

T

E

L

V

A

-

P

P

T

A

C

V

L

Y

R

A

G

G

M

L

S

E

R

R

K

K

A

Q

A

R

Q

L

Q

L

Q

R

A

A

L

Q

Q

E

L

L

L

L

E

Q

K

R

V

L

G

G

L

L

A

E

H

D

K

R

A

T

K

E

A

Y

Y

Y

P

P

G

A

T

Q

L

L

S

S

G

G

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

M

N

E

G

P

G

Q

Q

V

V

M

I

L

L

F

A

D
|
D

E

E

P

P

T

T

S

G

A

A

L

L

D

D

P

S

E

H

L

S

V

G

G

E

E

E

V

V

L

M

Q

A

V

I

I

L

R

H

A

Q

L

L

A

R

A

D

E

R

G

G

M

H

T

T

M

V

I

I

I

I

V

V

T

T

H

H

E

D

M

P

G

Q

F

V

A

A

R

A

E

Q

V

A

A

E

D

R

V

V

V

I

V

E

V

I

M

R

D

D

H

-

G

G

S

E

I

I

V

V

E

R

A

-

G

N

P

P

P

P

K47 (= K44) mutation to L: Lack of activity.
D169 (= D170) mutation to N: Lack of activity.

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
39% identity, 90% coverage: 6:236/257 of query aligns to 4:225/369 of P19566

query
sites
P19566

V

V

E

Q

V

L

S

R

G

N

L

V

R

T

K

K

S

A

F

W

G

G

A

D

H

V

V

V

V

V

L

S

K

K

D

D

L

I

D

N

L

L

S

D

V

I

A

H

P

D

S

G

Q

E

V

F

V

V

V

V

V

F

I

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

S

S

T

T

F

L

L

L

R

R

C

M

L

I

N

A

G

G

L

L

E

E

K

T

A

I

E

T

G

S

G

G

T

D

V

L

K

F

V

I

C

G

G

-

R

-

P

-

V

-

V

-

E

-

G

-

G

-

R

-

M

-

M

-

P

E

E

T

A

R

M

M

L

N

D

D

L

I

L

P

R

P

A

A

E

E

-

R

-

G

V

V

G

G

M

M

V

V

F

F

Q

Q

S

S

F

Y

N

A

L
|
L

F

Y

P

P

H

H

L

L

T

S

V

V

L

A

D

E

N

N

I

M

T

S

L

F

A

G

P

-

T

L

C

K

L

L

R

A

G

G

M

A

S

K

R

K

K

E

A

V

A

M

Q

N

Q

Q

Q

R

A

V

L

N

Q

Q

L

V

L

A

E

E

K

V

V

L

G

Q

L

L

A

A

H

H

K

L

A

L

K

E

A

R

Y

K

P

P

G

K

T

A

L

L

S

S

G

G

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

A

V

M

A

E

E

P

P

Q

R

V

V

M

F

L

L

F

L

D

D

E

E

P
|
P

T

L

S

S

A

N

L

L

D
|
D

P

A

E

A

L

L

-

R

V

V

G

Q

E

M

V

R

L

I

Q

E

V

I

I

S

R

R

A

L

L

H

A

K

A

R

E

L

G

G

M

R

T

T

M

M

I

I

I

Y

V

V

T

T

H

H

E

D

M

Q

G

V

F

E

A

A

R

M

E

T

V

L

A

A

D

D

V

K

V

I

V

V

V

V

M

L

D

D

H

A

G

G

S

R

I

V

V

A

E

Q

A

V

G

G

P

K

P

P

G

L

A

E

I

L

F

Y

T

H

A

Y

P

P

L86 (= L97) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P172) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D177) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
39% identity, 90% coverage: 6:236/257 of query aligns to 4:225/371 of P68187

query
sites
P68187

V

V

E

Q

V

L

S

Q

G

N

L

V

R

T

K

K

S

A

F

W

G

G

A

E

H

V

V

V

V

V

L

S

K

K

D

D

L

I

D

N

L

L

S

D

V

I

A

H

P

E

S

G

Q

E

V

F

V

V

V

V

V

F

I

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

S

S

T

T

F

L

L

L

R

R

C

M

L

I

N

A

G

G

L

L

E

E

K

T

A

I

E

T

G

S

G

G

T

D

V

L

K

F

V

I

C

G

G

E

R

K

P

-

V

-

V

-

E

-

G

-

G

-

R

-

M

-

M

-

P

-

E

-

A

R

M

M

L

N

D

D

L

T

L

P

R

P

A

A

E

E

-

R

-

G

V

V

G

G

M

M

V

V

F

F

Q

Q

S

S

F

Y

N
x
A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

L

A

D

E

N

N

I

M

T

S

L

F

A

G

P

-

T

L

C

K

L

L

R

A

G

G

M

A

S
x
K

R

K

K

E

A

V

A

I

Q

N

Q

Q

Q

R

A
x
V

L

N

Q

Q

L
x
V

L

A

E
|
E

K

V

V

L

G

Q

L

L

A
|
A

H

H

K

L

A

L

K

D

A

R

Y

K

P

P

G

K

T

A

L

L

S

S

G

G

G
|
G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

L

L

A

V

M

A

E

E

P

P

Q

S

V

V

M

F

L

L

F

L

D
|
D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

P

A

E

A

L

L

-

R

V

V

G

Q

E

M

V

R

L

I

Q

E

V

I

I

S

R

R

A

L

L

H

A

K

A

R

E

L

G

G

M

R

T

T

M

M

I

I

I

Y

V

V

T

T

H

H

E

D

M

Q

G

V

F

E

A

A

R

M

E

T

V

L

A

A

D

D

V

K

V

I

V

V

V

V

M

L

D

D

H

A

G

G

S

R

I

V

V

A

E

Q

A

V

G

G

P

K

P

P

G

L

A

E

I

L

F

Y

T

H

A

Y

P

P

A85 (≠ N96) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ S118) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ A126) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L129) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E131) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.