SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS12030 FitnessBrowser__HerbieS:HSERO_RS12030 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3uf6A The crystal structure of a possible phosphate acetyl/butaryl transferase (from listeria monocytogenes egd-e) in complex with cod (3'-dephosphocoenzyme a)
41% identity, 44% coverage: 255:468/484 of query aligns to 66:276/285 of 3uf6A

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binding dephospho coenzyme a: T92 (= T281), A133 (= A321), M134 (≠ V322), Y239 (≠ A431), K240 (= K432), S252 (≠ G444), I263 (≠ L455), S265 (= S457)

3u9eB The crystal structure of a possible phosphate acetyl/butaryl transferase (from listeria monocytogenes egd-e) in complex with coa.
41% identity, 44% coverage: 255:468/484 of query aligns to 68:278/288 of 3u9eB

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binding arginine: N238 (= N428), K242 (= K432), S266 (≠ T456), S267 (= S457), D270 (= D460)
binding coenzyme a: T94 (= T281), A95 (≠ E282), L102 (≠ V289), K103 (≠ A290), R109 (≠ H295), L113 (≠ R299), L114 (≠ M300), M136 (≠ V322), E174 (≠ T360), K216 (= K406), I265 (≠ L455), S267 (= S457), R268 (= R458)

P86397 Hydroxyacyl-thioester dehydratase type 2, mitochondrial; HsHTD2; 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; EC 4.2.1.59 from Homo sapiens (Human) (see paper)
40% identity, 27% coverage: 32:161/484 of query aligns to 35:163/168 of P86397

query
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P86397

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D62 (= D59) mutation to A: Abolishes ability to complement a htd2-delta mutant yeast.
H67 (= H64) mutation to A: Abolishes ability to complement a htd2-delta mutant yeast.

Sites not aligning to the query:

1 modified: transit peptide, Mitochondrion
168 modified: mature protein, Hydroxyacyl-thioester dehydratase type 2, mitochondrial

O32472 (R)-specific enoyl-CoA hydratase; EC 4.2.1.119 from Aeromonas caviae (Aeromonas punctata) (see 2 papers)
41% identity, 27% coverage: 33:161/484 of query aligns to 6:134/134 of O32472

query
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O32472

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D32 (= D59) mutation to A: Almost no activity.
H37 (= H64) mutation H->A,N: Almost no activity.
S63 (= S90) mutation to A: 100-fold lower Vmax.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

A0A3Q7HWE4 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FERN, mitochondrial; 3-hydroxyl-ACP dehydratase FERN; Protein FERN-LIKE; SlFERN; EC 4.2.1.59 from Solanum lycopersicum (Tomato) (Lycopersicon esculentum) (see paper)
33% identity, 27% coverage: 33:162/484 of query aligns to 23:152/165 of A0A3Q7HWE4

query
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C133 (= C143) mutation to Y: In fern-like; pleiotropic phenotypes associated with lower mitochondrial fatty acid synthesis (mtFAS) and reduced chlorophyll content such as dwarfism, yellowing, curly leaves, and increased axillary buds. New leaves looks like a spiral fern. Reduced accumulation (at protein level).

1xcoD Crystal structure of a phosphotransacetylase from bacillus subtilis in complex with acetylphosphate (see paper)
24% identity, 54% coverage: 199:458/484 of query aligns to 19:306/325 of 1xcoD

query
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1xcoD

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binding acetylphosphate: R90 (vs. gap), R91 (vs. gap), K94 (= K270), T131 (vs. gap), K143 (≠ H295), Y278 (≠ A431), K279 (= K432), N298 (≠ R450), S305 (= S457), R306 (= R458)

6jqoA Structure of paaz, a bifunctional enzyme in complex with NADP+ and ccoa (see paper)
36% identity, 21% coverage: 28:130/484 of query aligns to 528:632/678 of 6jqoA

query
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L

S

S

G

G

D

D

A

H

N

F

P

Y

Q

A

H

H

L

M

D

D

L

K

A

I

F

A

A

A

K

A

E

E

T

S

R

I

F

F

H

G

G

E

L

R

I

V

A

V

H

H

G

G

M

Y

F

F

-

V

-

L

-

S

-

A

G

A

G

G

A

L

L

F

I

V

S

D

A

A

V

G

L

V

G

G

T

P

R

V

L

I

P

A

G

N

P

Y

G

G

T

L

I

-

Y

-

L

-

G

-

Q

E

T

S

L

L

R

R

F

F

V

I

A

E

P

P

V

V

R

K

I

P

G

G

D

D

T

T

L

I

R

Q

T

V

T

R

V

L

T

T

V

C

K

K

E

R

R

K

Sites not aligning to the query:

active site: 157, 255, 294, 483
binding crotonyl coenzyme a: 97, 107, 111, 158, 161, 638
binding nadp nicotinamide-adenine-dinucleotide phosphate: 154, 156, 157, 183, 230, 231, 232, 235, 256, 257, 294

6jqnA Structure of paaz, a bifunctional enzyme in complex with NADP+ and ocoa (see paper)
36% identity, 21% coverage: 28:130/484 of query aligns to 528:632/678 of 6jqnA

query
sites
6jqnA

R

K

I

Y

F

F

S

E

E

E

I

L

A

Q

V

P

G

G

D

D

S

S

A

L

-

L

T

T

I

P

T

R

R

R

T

T

L

M

T

T

L

E

A

A

D

D

I

I

Q

V

L

N

F

F

A

A

V

C

I

L

S

S

G

G

D

D

A

H

N
x
F

P

Y

Q

A

H
|
H

L

M

D

D

L

K

A

I

F

A

A

A

K

A

E

E

T

S

R

I

F
|
F

H

G

G

E

L

R

I

V

A

V

H

H

G
|
G

M

Y

F

F

-

V

-

L

-

S

-

A

G

A

G

G

A

L

L

F

I
x
V

S

D

A

A

V

G

L

V

G

G

T

P

R

V

L

I

P
x
A

G
x
N

P
x
Y

G

G

T

L

I

-

Y

-

L

-

G

-

Q

E

T

S

L

L

R

R

F
|
F

V
x
I

A

E

P

P

V

V

R

K

I

P

G

G

D

D

T

T

L

I

R

Q

T

V

T

R

V

L

T

T

V

C

K

K

E

R

R

K

binding octanoyl-coenzyme a: F562 (≠ N61), H565 (= H64), F576 (= F75), G583 (= G82), V595 (≠ I89), A604 (≠ P98), N605 (≠ G99), Y606 (≠ P100), F613 (= F111), I614 (≠ V112)

Sites not aligning to the query:

active site: 157, 255, 294, 483
binding nadp nicotinamide-adenine-dinucleotide phosphate: 19, 153, 154, 155, 156, 180, 182, 183, 230, 231, 232, 235, 239, 255, 256, 257, 294, 396, 471

6jqmA Structure of paaz with NADPH (see paper)
36% identity, 21% coverage: 28:130/484 of query aligns to 528:632/678 of 6jqmA

query
sites
6jqmA

R

K

I

Y

F

F

S

E

E

E

I

L

A

Q

V

P

G

G

D

D

S

S

A

L

-

L

T

T

I

P

T

R

R

R

T

T

L

M

T

T

L

E

A

A

D

D

I

I

Q

V

L

N

F

F

A

A

V

C

I

L

S

S

G

G

D

D

A

H

N

F

P

Y

Q

A

H

H

L

M

D

D

L

K

A

I

F

A

A

A

K

A

E

E

T

S

R

I

F

F

H

G

G

E

L

R

I

V

A

V

H

H

G

G

M

Y

F

F

-

V

-

L

-

S

-

A

G

A

G

G

A

L

L

F

I

V

S

D

A

A

V

G

L

V

G

G

T

P

R

V

L

I

P

A

G

N

P

Y

G

G

T

L

I

-

Y

-

L

-

G

-

Q

E

T

S

L

L

R

R

F

F

V

I

A

E

P

P

V

V

R

K

I

P

G

G

D

D

T

T

L

I

R

Q

T

V

T

R

V

L

T

T

V

C

K

K

E

R

R

K

Sites not aligning to the query:

active site: 157, 255, 294, 483
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 19, 153, 154, 155, 156, 157, 180, 182, 183, 231, 232, 235, 256, 257, 294, 394, 396

P77455 Bifunctional protein PaaZ; EC 3.3.2.12; EC 1.2.1.91 from Escherichia coli (strain K12) (see paper)
36% identity, 21% coverage: 28:130/484 of query aligns to 529:633/681 of P77455

query
sites
P77455

R

K

I

Y

F

F

S

E

E

E

I

L

A

Q

V

P

G

G

D

D

S

S

A

L

-

L

T

T

I

P

T

R

R

R

T

T

L

M

T

T

L

E

A

A

D

D

I

I

Q

V

L

N

F

F

A

A

V

C

I

L

S

S

G

G

D

D

A

H

N

F

P

Y

Q

A

H

H

L

M

D

D

L

K

A

I

F

A

A

A

K

A

E

E

T

S

R

I

F

F

H

G

G

E

L

R

I

V

A

V

H

H

G

G

M

Y

F

F

-

V

-

L

-

S

-

A

G

A

G

G

A

L

L

F

I

V

S

D

A

A

V

G

L

V

G

G

T

P

R

V

L

I

P

A

G

N

P

Y

G

G

T

L

I

-

Y

-

L

-

G

-

Q

E

T

S

L

L

R

R

F

F

V

I

A

E

P

P

V

V

R

K

I

P

G

G

D

D

T

T

L

I

R

Q

T

V

T

R

V

L

T

T

V

C

K

K

E

R

R

K

Sites not aligning to the query:

256 E→Q: Catalyzes the formation of 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde instead of 3-oxo-5,6-dehydrosuberyl-CoA.

Q8ZND6 Phosphate acetyltransferase; Phosphotransacetylase; EC 2.3.1.222; EC 2.3.1.8 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
26% identity, 40% coverage: 265:458/484 of query aligns to 499:691/714 of Q8ZND6

query
sites
Q8ZND6

L

M

V

L

M

E

H

Q

G

D

K

E

V

V

E

D

M

G

L

L

M

V

K

S

G

G

S

A

L

V

H

H

T

T

E

T

E

A

F

N

-

T

-

I

-

R

-

P

M

L

G

Q

A

L

V

I

V

K

A

T

C

A

P

P

G

G

L

-

H

-

T

-

K

S

R

S

R

L

M

V

S

S

H

S

C

V

Y

F

L

F

M

M

Q

L

T

L

P

P

S

E

Y

-

P

-

R

Q

P

V

F

Y

I

V

I

Y

T

G

D

D

A

C

A

A

V

I

N

N

I

P

E

D

P

P

G

T

L

A

A

E

E

Q

K

L

A

A

D

E

I

I

I

A

R

I

N

Q

A

S

I

A

E

D

L

S

A

A

H

I

V

A

I

F

G

G

V

I

K

E

T

-

P

P

K

R

V

V

A

A

I

M

L

L

A

S

A

Y

V

S

E

T

T

G

V

T

N

S

P

G

R

A

M

G

P

S

A

D

T

V

L

E

D

K

A

V

A

R

A

E

L

A

C

T

K

R

M

L

A

A

D

Q

R

E

G

K

Q

R

I

-

T

P

G

D

A

L

I

M

L

I

D

D

G

G

P

P

L

L

A

Q

F

Y

D

D

N

A

A

A

V

V

S

M

P

A

V

D

S

V

A

A

R

K

V

S

K

K

G

A

I

P

R

N

S

S

E

P

V

V

A

A

G

G

Q

R

A

A

D

T

I

V

L

F

V

I

V

F

P

P

D

D

L

L

E

N

S

T

G

G

N

N

L

T

A

Y

K

K

Q

A

L

V

E

Q

Y

R

M

S

G

A

G

D

A

L

I

I

S

S

A

I

G

G

L

P

V

M

L

L

-

Q

G

G

A

M

R

R

I

K

P

P

L

V

I

N

L

D

T

L

S

S

R

R

Sites not aligning to the query:

252 R→H: Increases speed of forward and back reactions by 2-3 fold. Not inhibited by NADH.
273 G→D: Increases speed of forward and back reactions by 2-3 fold.
294 M→I: Slightly decreases speed of forward and back reactions.

8ps2G Asymmetric unit of the yeast fatty acid synthase with acp at the enoyl reductase domain (fasam sample) (see paper)
32% identity, 27% coverage: 33:161/484 of query aligns to 1525:1646/2036 of 8ps2G

query
sites
8ps2G

I

I

A

A

V

V

G

L

D

D

S

S

A

Y

T

T

I

P

T

S

R

T

T

N

L

-

T

-

L

-

A

-

D

-

I

-

Q

E

L

P

F

Y

A

A

V

R

I

V

S

S

G

G

D

D

A

L

N

N

P

P

Q

I

H

H

L

V

D

S

L

R

A

H

F

F

A

A

K

S

E

Y

T

A

R

N

F

L

H

P

G

G

L

T

I

I

A

T

H

H

G

G

M

M

F

F

G

S

A

A

L

S

I

V

S

R

A

A

V

L

L

I

G

E

T

N

R

W

L

A

P

A

G

D

P

S

G

V

T

S

I

S

Y

R

L

V

-

R

G

G

Q

Y

T

T

L

C

R

Q

F

F

V

V

A

D

P

M

V

V

R

L

I

P

G

N

D

T

T

A

L

L

R

K

T

T

T

S

V

I

T

Q

V

H

K

V

E

G

R

M

D

I

E

N

A

G

R

R

K

K

L

L

L

I

T

K

L

F

A

E

C

T

A

R

C

-

I

-

N

N

Q

E

D

D

G

D

V

V

T

V

V

V

I

L

E

T

G

G

E

E

A

A

K

E

V

I

L

E

A

Q

P

P

Sites not aligning to the query:

binding ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (~{E})-but-2-enethioate: 679, 736, 738
binding flavin mononucleotide: 591, 592, 593, 594, 646, 648, 702, 733, 734, 765, 796, 798, 799, 802, 1050
binding malonyl-coenzyme a: 1655, 1793, 1794, 1795, 1820, 1844, 1847, 1876, 1878, 1883, 1948, 1950, 1951, 1952, 1953, 1955, 1962, 1963

8prwG Fatty acid synthase subunit beta (see paper)
32% identity, 27% coverage: 33:161/484 of query aligns to 1523:1644/2034 of 8prwG

query
sites
8prwG

I

I

A

A

V

V

G

L

D

D

S

S

A

Y

T

T

I

P

T

S

R

T

T

N

L

-

T

-

L

-

A

-

D

-

I

-

Q

E

L

P

F

Y

A

A

V

R

I

V

S

S

G

G

D

D

A

L

N

N

P

P

Q

I

H

H

L

V

D

S

L

R

A

H

F

F

A

A

K

S

E

Y

T

A

R

N

F

L

H

P

G

G

L

T

I

I

A

T

H

H

G

G

M

M

F

F

G

S

A

A

L

S

I

V

S

R

A

A

V

L

L

I

G

E

T

N

R

W

L

A

P

A

G

D

P

S

G

V

T

S

I

S

Y

R

L

V

-

R

G

G

Q

Y

T

T

L

C

R

Q

F

F

V

V

A

D

P

M

V

V

R

L

I

P

G

N

D

T

T

A

L

L

R

K

T

T

T

S

V

I

T

Q

V

H

K

V

E

G

R

M

D

I

E

N

A

G

R

R

K

K

L

L

L

I

T

K

L

F

A

E

C

T

A

R

C

-

I

-

N

N

Q

E

D

D

G

D

V

V

T

V

V

V

I

L

E

T

G

G

E

E

A

A

K

E

V

I

L

E

A

Q

P

P

Sites not aligning to the query:

binding coenzyme a: 1791, 1792, 1838, 1840, 1842, 1845, 1874, 1876, 1881, 1946, 1950, 1951
binding 1-deoxy-1-(7,8-dimethyl-2,4-dioxo-3,4-dihydro-2h-benzo[g]pteridin-1-id-10(5h)-yl)-5-o-phosphonato-d-ribitol: 591, 592, 593, 594, 646, 702, 733, 734, 765, 798, 799, 802, 1050
binding nadp nicotinamide-adenine-dinucleotide phosphate: 594, 618, 621, 648, 651, 736, 845, 1008, 1009, 1010, 1027, 1028, 1030, 1050

8prvG Asymmetric unit of the yeast fatty acid synthase in the non-rotated state with acp at the ketosreductase domain (fasamn sample) (see paper)
32% identity, 27% coverage: 33:161/484 of query aligns to 1523:1644/2034 of 8prvG

query
sites
8prvG

I

I

A

A

V

V

G

L

D

D

S

S

A

Y

T

T

I

P

T

S

R

T

T

N

L

-

T

-

L

-

A

-

D

-

I

-

Q

E

L

P

F

Y

A

A

V

R

I

V

S

S

G

G

D

D

A

L

N

N

P

P

Q

I

H

H

L

V

D

S

L

R

A

H

F

F

A

A

K

S

E

Y

T

A

R

N

F

L

H

P

G

G

L

T

I

I

A

T

H

H

G

G

M

M

F

F

G

S

A

A

L

S

I

V

S

R

A

A

V

L

L

I

G

E

T

N

R

W

L

A

P

A

G

D

P

S

G

V

T

S

I

S

Y

R

L

V

-

R

G

G

Q

Y

T

T

L

C

R

Q

F

F

V

V

A

D

P

M

V

V

R

L

I

P

G

N

D

T

T

A

L

L

R

K

T

T

T

S

V

I

T

Q

V

H

K

V

E

G

R

M

D

I

E

N

A

G

R

R

K

K

L

L

L

I

T

K

L

F

A

E

C

T

A

R

C

-

I

-

N

N

Q

E

D

D

G

D

V

V

T

V

V

V

I

L

E

T

G

G

E

E

A

A

K

E

V

I

L

E

A

Q

P

P

Sites not aligning to the query:

binding coenzyme a: 1792, 1838, 1841, 1842, 1845, 1874, 1876, 1881, 1946, 1949, 1950, 1951, 1953
binding flavin mononucleotide: 591, 592, 593, 594, 646, 702, 733, 734, 766, 798, 799, 1050
binding nadp nicotinamide-adenine-dinucleotide phosphate: 621, 736, 1010, 1027, 1028

6u5uG Electron cryomicroscopy structure of s. Cerevisiae fas in the ks- stalled state (see paper)
32% identity, 27% coverage: 33:161/484 of query aligns to 1522:1643/2033 of 6u5uG

query
sites
6u5uG

I

I

A

A

V

V

G

L

D

D

S

S

A

Y

T

T

I

P

T

S

R

T

T

N

L

-

T

-

L

-

A

-

D

-

I

-

Q

E

L

P

F

Y

A

A

V

R

I

V

S

S

G

G

D

D

A

L

N

N

P

P

Q

I

H

H

L

V

D

S

L

R

A

H

F

F

A

A

K

S

E

Y

T

A

R

N

F

L

H

P

G

G

L

T

I

I

A

T

H

H

G

G

M

M

F

F

G

S

A

A

L

S

I

V

S

R

A

A

V

L

L

I

G

E

T

N

R

W

L

A

P

A

G

D

P

S

G

V

T

S

I

S

Y

R

L

V

-

R

G

G

Q

Y

T

T

L

C

R

Q

F

F

V

V

A

D

P

M

V

V

R

L

I

P

G

N

D

T

T

A

L

L

R

K

T

T

T

S

V

I

T

Q

V

H

K

V

E

G

R

M

D

I

E

N

A

G

R

R

K

K

L

L

L

I

T

K

L

F

A

E

C

T

A

R

C

-

I

-

N

N

Q

E

D

D

G

D

V

V

T

V

V

V

I

L

E

T

G

G

E

E

A

A

K

E

V

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L

E

A

Q

P

P

Sites not aligning to the query:

binding flavin mononucleotide: 591, 592, 593, 594, 702, 729, 733, 765, 766, 798, 799, 802
binding nadp nicotinamide-adenine-dinucleotide phosphate: 594, 621, 653, 736, 1007, 1008, 1027, 1028, 1030

6ql5G Fatty acid synthase subunit beta (see paper)
32% identity, 27% coverage: 33:161/484 of query aligns to 1525:1646/2036 of 6ql5G

query
sites
6ql5G

I

I

A

A

V

V

G

L

D

D

S

S

A

Y

T

T

I

P

T

S

R

T

T

N

L

-

T

-

L

-

A

-

D

-

I

-

Q

E

L

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F

Y

A

A

V

R

I

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S

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G

D

D

A

L

N

N

P

P

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I

H

H

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V

D

S

L

R

A

H

F

F

A

A

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Y

T

A

R

N

F

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H

P

G

G

L

T

I

I

A

T

H

H

G

G

M

M

F

F

G

S

A

A

L

S

I

V

S

R

A

A

V

L

L

I

G

E

T

N

R

W

L

A

P

A

G

D

P

S

G

V

T

S

I

S

Y

R

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-

R

G

G

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Y

T

T

L

C

R

Q

F

F

V

V

A

D

P

M

V

V

R

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N

D

T

T

A

L

L

R

K

T

T

T

S

V

I

T

Q

V

H

K

V

E

G

R

M

D

I

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N

A

G

R

R

K

K

L

L

L

I

T

K

L

F

A

E

C

T

A

R

C

-

I

-

N

N

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E

D

D

G

D

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V

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V

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E

T

G

G

E

E

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A

K

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P

Sites not aligning to the query:

binding flavin mononucleotide: 591, 592, 593, 594, 646, 702, 733, 734, 765, 798, 799, 1050
binding 4'-phosphopantetheine: 159, 160, 161, 270, 334, 366, 368, 506

7tuiB Structure of c. Albicans fas in an inhibited state
30% identity, 23% coverage: 53:162/484 of query aligns to 1535:1644/2033 of 7tuiB

query
sites
7tuiB

F

Y

A

A

V

I

I

V

S

S

G

G

D

D

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Y

N

N

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P

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G

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I

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T

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M

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Y

G

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A

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S

R

A

A

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L

V

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E

T

E

R

W

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A

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N

P

N

G

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A

I

A

Y

R

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V

-

R

G

A

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F

T

K

L

C

R

D

F

F

V

V

A

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M

V

V

R

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I

P

G

N

D

D

T

T

L

L

R

Q

T

T

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M

T

E

V

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V

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M

D

I

E

N

A

G

R

R

K

K

L

I

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K

L

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A

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C

T

A

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C

N

I

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N

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-

V

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G

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E

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T

Sites not aligning to the query:

binding flavin mononucleotide: 578, 579, 580, 581, 582, 689, 720, 721, 722, 752, 753, 785, 786, 789, 1038

6u5wB Electron cryomicroscopy structure of c. Albicans fas in the ks-stalled state (see paper)
30% identity, 23% coverage: 53:162/484 of query aligns to 1535:1644/2033 of 6u5wB

query
sites
6u5wB

F

Y

A

A

V

I

I

V

S

S

G

G

D

D

A

Y

N

N

P

P

Q

I

H

H

L

V

D

S

L

R

A

V

F

F

A

A

K

A

E

Y

T

A

R

K

F

L

H

P

G

G

L

T

I

I

A

T

H

H

G

G

M

M

F

Y

G

S

A

A

L

S

I

I

S

R

A

A

V

L

L

V

G

E

T

E

R

W

L

A

P

A

G

N

P

N

G

V

T

A

I

A

Y

R

L

V

-

R

G

A

Q

F

T

K

L

C

R

D

F

F

V

V

A

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P

M

V

V

R

L

I

P

G

N

D

D

T

T

L

L

R

Q

T

T

V

M

T

E

V

H

K

V

E

G

R

M

D

I

E

N

A

G

R

R

K

K

L

I

T

K

L

V

A

E

C

T

A

R

C

N

I

V

N

E

Q

T

D

E

G

-

V

L

T

P

V

V

I

L

E

I

G

G

E

E

A

A

K

E

V

I

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A

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P

T

T

Sites not aligning to the query:

binding flavin mononucleotide: 579, 580, 581, 689, 716, 720, 752, 753, 785, 786, 789
binding nadp nicotinamide-adenine-dinucleotide phosphate: 608, 640, 722, 723, 995, 996, 997, 1015, 1016, 1018

7svtB Mycobacterium tuberculosis 3-hydroxyl-acp dehydratase hadab in complex with 1,3-diarylpyrazolyl-acylsulfonamide inhibitor (see paper)
36% identity, 23% coverage: 28:140/484 of query aligns to 3:120/141 of 7svtB

query
sites
7svtB

R

R

I

E

F

F

S

S

E

S

I

V

A

K

V

V

G

G

D

D

S

Q

-

L

A

P

T

E

I

K

T

T

R

Y

T

P

L

L

T

T

L

R

A

Q

D

D

I

L

Q

V

L

N

F

Y

A

A

V

G

I

V

S

S

G

G

D

D

A

L

N

N

P

P

Q

I

H
|
H

L

W

D

D

L

D

A

E

F

I

A

A

K

K

E

V

T

V

R

G

F

L

H

D

G

T

L

A

I

I

A

A

H
|
H

G

G

M

M

F

L

-

T

-

M

-

G

-

I

G

G

A

G

L

Y

I

V

S

T

A

S

V

W

L

V

G

G

T

D

R

-

L

-

P

P

G

G

P

A

G

V

T

T

I

E

Y

Y

L

-

G

-

Q

-

T

N

L

V

R

R

F

F

V

T

A

A

P

V

V

V

R

P

I

V

-

P

-

N

-

D

-

G

-

K

G

G

D

A

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E

L

L

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F

T

N

V

G

T

R

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V

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A

binding 3-[1-(4-bromophenyl)-3-(4-chlorophenyl)-1H-pyrazol-4-yl]-N-(methanesulfonyl)propanamide: H40 (= H64), H57 (= H81)

4v8lD Structure of the Mycobacterial Fatty Acid Synthase (see paper)
39% identity, 15% coverage: 49:121/484 of query aligns to 1205:1279/2822 of 4v8lD

query
sites
4v8lD

D

D

I

M

Q

R

L

P

F

F

A

A

V

V

I

V

S

S

G

G

D

D

A

H

N

N

P

P

Q

I

H

H

L

T

D

D

L

R

A

A

F

A

A

A

K

L

E

L

T

A

R

G

F

L

H

E

G

G

L

P

I

I

A

V

H

H

G

G

M

M

F

W

G

L

G

S

A

A

-

A

-

Q

-

H

L

V

I

V

S

T

A

A

V

T

L

D

G

G

T

K

R

-

L

-

P

P

G

V

P

P

G

P

T

A

I

K

Y

L

L

I

G

G

Q

W

T

T

L

A

R

R

F

F

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L

A

G

P

M

V

V

R

K

I

P

G

G

D

D

T

Q

L

V

Sites not aligning to the query:

active site: 2473, 2625, 2636, 2661, 2666, 2730, 2732
binding flavin mononucleotide: 404, 405, 406, 407, 460, 493, 520, 547, 551, 583, 584, 616, 617, 862

Query Sequence

>HSERO_RS12030 FitnessBrowser__HerbieS:HSERO_RS12030
MADSLPAPVVADKDAHASDDVLGLIRNRIFSEIAVGDSATITRTLTLADIQLFAVISGDA
NPQHLDLAFAKETRFHGLIAHGMFGGALISAVLGTRLPGPGTIYLGQTLRFVAPVRIGDT
LRTTVTVKERDEARKLLTLACACINQDGVTVIEGEAKVLAPTEPILRRRTSLPEVSMKMG
DADLQLLLEHARKPGPAPVAVVHPCDELSLAAVLDAHAAGLISPILVAPRSRLLALAESH
KLDISAFPLEDVPHSHAAAARAIELVMHGKVEMLMKGSLHTEEFMGAVVACPGLHTKRRM
SHCYLMQTPSYPRPFIITDAAVNIEPGLAEKADIIRNAIELAHVIGVKTPKVAILAAVET
VNPRMPATLDAAALCKMADRGQITGAILDGPLAFDNAVSPVSARVKGIRSEVAGQADILV
VPDLESGNLLAKQLEYMGGAISAGLVLGARIPLILTSRADSRETRLASCAVARMLAQHYR
SAPP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory