SitesBLAST
Comparing HSERO_RS13375 FitnessBrowser__HerbieS:HSERO_RS13375 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5i1fA Crystal structure of utp-glucose-1-phosphate uridylyltransferase from burkholderia vietnamiensis in complex with uridine-5'-diphosphate- glucose
63% identity, 86% coverage: 1:287/335 of query aligns to 2:285/290 of 5i1fA
- binding uridine-5'-diphosphate-glucose: P11 (= P10), A13 (= A12), G14 (= G13), K28 (= K27), E29 (= E28), Q106 (= Q105), A109 (≠ P108), L110 (= L109), G111 (= G110), L112 (= L111), A115 (= A114), D135 (= D134), Y172 (= Y174), G173 (= G175), E192 (= E194), K193 (= K195), V205 (= V207)
5ve7A Crystal structure of utp-glucose-1-phosphate uridylyltransferase from burkholderia ambifaria in complex with utp
63% identity, 85% coverage: 3:288/335 of query aligns to 2:280/282 of 5ve7A
- binding uridine 5'-triphosphate: P9 (= P10), V10 (= V11), A11 (= A12), G12 (= G13), G14 (= G15), T15 (≠ S16), R16 (= R17), K26 (= K27), E27 (= E28), Q104 (= Q105), A107 (≠ P108), G109 (= G110), A113 (= A114)
2ux8G Crystal structure of sphingomonas elodea atcc 31461 glucose-1- phosphate uridylyltransferase in complex with glucose-1-phosphate. (see paper)
52% identity, 83% coverage: 1:279/335 of query aligns to 2:275/288 of 2ux8G
8f73E Crystal structure of pseudomonas aeruginosa udp-glucose phosphorylase in complex with udp-glucose
52% identity, 79% coverage: 4:269/335 of query aligns to 8:271/281 of 8f73E
- binding uridine-5'-diphosphate-glucose: P14 (= P10), A16 (= A12), G17 (= G13), K31 (= K27), E32 (= E28), Q108 (= Q105), G113 (= G110), L114 (= L111), A117 (= A114), L134 (= L131), D137 (= D134), E196 (= E194), K197 (= K195), I209 (≠ V207)
6knlA Uridine and triphosphate-bound ugpase from acinetobacter baumannii
49% identity, 86% coverage: 4:290/335 of query aligns to 2:289/290 of 6knlA
- binding triphosphate: G13 (= G15), T14 (≠ S16), R15 (= R17), K79 (≠ A81), K81 (= K83)
- binding uridine: P8 (= P10), G11 (= G13), K25 (= K27), Q103 (= Q105), P106 (= P108), G108 (= G110), P130 (≠ A132), D131 (= D133)
6k8dA Udp-glucose pyrophosphorylase with upg from acinetobacter baumanii
49% identity, 86% coverage: 4:290/335 of query aligns to 2:289/290 of 6k8dA
- binding uridine-5'-diphosphate-glucose: P8 (= P10), A10 (= A12), G11 (= G13), Q103 (= Q105), P106 (= P108), G108 (= G110), L109 (= L111), A112 (= A114), L129 (= L131), D131 (= D133), E193 (= E194), V206 (= V207)
6ikzB Udp-glucose pyrophosphorylase from acinetobacter baumanii
48% identity, 86% coverage: 4:290/335 of query aligns to 2:284/285 of 6ikzB
- binding uridine 5'-triphosphate: P8 (= P10), V9 (= V11), A10 (= A12), G11 (= G13), L12 (= L14), G13 (= G15), T14 (≠ S16), R15 (= R17), K25 (= K27), Q103 (= Q105), P106 (= P108), G108 (= G110), D131 (= D133)
3jukA The crystal structure of udp-glucose pyrophosphorylase complexed with udp-glucose (see paper)
47% identity, 80% coverage: 4:271/335 of query aligns to 2:259/265 of 3jukA
- binding uridine-5'-diphosphate-glucose: A10 (= A12), G11 (= G13), E26 (= E28), Q94 (= Q105), M97 (≠ P108), G99 (= G110), L100 (= L111), A103 (= A114), D123 (= D134), Y162 (= Y174), G163 (= G175), E182 (= E194), K183 (= K195), V195 (= V207)
3jukD The crystal structure of udp-glucose pyrophosphorylase complexed with udp-glucose (see paper)
47% identity, 80% coverage: 4:271/335 of query aligns to 2:259/264 of 3jukD
- binding magnesium ion: T14 (≠ S16), R15 (= R17)
- binding uridine-5'-diphosphate-glucose: P8 (= P10), A10 (= A12), G11 (= G13), E26 (= E28), Q94 (= Q105), M97 (≠ P108), G99 (= G110), L100 (= L111), A103 (= A114), L120 (= L131), D123 (= D134), Y162 (= Y174), G163 (= G175), E182 (= E194), K183 (= K195), V195 (= V207)
2ux8A Crystal structure of sphingomonas elodea atcc 31461 glucose-1- phosphate uridylyltransferase in complex with glucose-1-phosphate. (see paper)
45% identity, 83% coverage: 1:279/335 of query aligns to 2:242/255 of 2ux8A
2pa4B Crystal structure of udp-glucose pyrophosphorylase from corynebacteria glutamicum in complex with magnesium and udp-glucose (see paper)
40% identity, 87% coverage: 4:295/335 of query aligns to 3:294/299 of 2pa4B
- binding uridine-5'-diphosphate-glucose: P9 (= P10), A10 (≠ V11), A11 (= A12), G12 (= G13), E27 (= E28), Q103 (= Q105), P106 (= P108), G108 (= G110), L109 (= L111), L131 (= L131), P132 (≠ A132), D134 (= D134), Y170 (= Y174), G171 (= G175), E192 (= E194), K193 (= K195), A205 (≠ V207)
8b6dA Crystal structure of udp-glucose pyrophosphorylase from thermocrispum agreste dsm 44070 in complex with udp
40% identity, 87% coverage: 4:293/335 of query aligns to 2:290/291 of 8b6dA
- binding uridine-5'-diphosphate: P8 (= P10), A10 (= A12), G11 (= G13), L12 (= L14), G13 (= G15), T14 (≠ S16), R15 (= R17), K25 (= K27), Q102 (= Q105), A105 (≠ P108), G107 (= G110), A111 (= A114)
8b68A Crystal structure of udp-glucose pyrophosphorylase from thermocrispum agreste dsm 44070 in complex with udp-glucose
38% identity, 87% coverage: 4:293/335 of query aligns to 2:285/286 of 8b68A
- binding uridine-5'-diphosphate-glucose: P8 (= P10), A10 (= A12), G11 (= G13), Q102 (= Q105), A105 (≠ P108), G107 (= G110), A111 (= A114), L130 (= L131), P131 (≠ A132), D133 (= D134), A203 (≠ V207), G205 (= G209)
2ggqA Complex of hypothetical glucose-1-phosphate thymidylyltransferase from sulfolobus tokodaii
29% identity, 76% coverage: 6:260/335 of query aligns to 2:210/401 of 2ggqA
- active site: R13 (= R17)
- binding thymidine-5'-triphosphate: L6 (≠ P10), A8 (= A12), G9 (= G13), S10 (≠ L14), G11 (= G15), E12 (≠ S16), R13 (= R17), K23 (= K27), Q73 (≠ R104), G79 (= G110), A83 (= A114), R179 (≠ G229), E181 (= E231)
Sites not aligning to the query:
Q975F9 Bifunctional sugar-1-phosphate nucleotidylyltransferase/acetyltransferase; EC 2.7.7.24; EC 2.7.7.9; EC 2.7.7.83; EC 2.7.7.23; EC 2.3.1.276; EC 2.3.1.157 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
29% identity, 76% coverage: 6:260/335 of query aligns to 2:210/401 of Q975F9
- AGSGER 8:13 (≠ AGLGSR 12:17) binding
- Q73 (≠ R104) binding
- G79 (= G110) binding
- T80 (≠ L111) mutation T->A,G,Q: Increases both GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation T->D,H: Decrease in GlcNAc-1-P UTase activity but increase in Glc-1-P UTase activity.; mutation T->E,K,L,M,R,W,Y: Strong decrease in GlcNAc-1-P UTase activity and loss of Glc-1-P UTase activity.; mutation T->F,I: Loss of both GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation T->N,S: Strong increase in GlcNAc-1-P UTase activity and decrease in Glc-1-P UTase activity.; mutation to N: Loss of GlcNAc-1-P UTase activity; when associated with V-97.
- Y97 (≠ L131) mutation Y->A,D,F,G,I,K,T,V: Increases GlcNAc-1-P UTase activity. Decreases Glc-1-P UTase activity.; mutation Y->C,E,P,R,W: Decreases GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation Y->H,L,M,N,Q,S: Increases GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation to V: Loss of GlcNAc-1-P UTase activity; when associated with N-80.
- E146 (= E194) mutation E->A,C,F,G,I,K,L,M,P,Q,R,V,W,Y: Loss of both GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation E->D,N: Decrease in GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation E->H,S,T: Decrease in GlcNAc-1-P UTase activity and loss of Glc-1-P UTase activity.
Sites not aligning to the query:
- 308 H→A: Strong decrease in GalN-1-P AcTase activity and almost loss of GlcN-1-P AcTase activity.
- 311 Y→A: Strong decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
- 331 N→A: Strong decrease in GalN-1-P AcTase activity and decrease in GlcN-1-P AcTase activity.
- 337 K→A: Slight decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
- 340 K→A: Decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
- 391:401 mutation Missing: No change in GlcNAc-1-P UTase activity. Shows 38% less GalN-1-P AcTase activity than the wild-type, but increases GlcN-1-P AcTase activity 16.8 times. Significantly affects the thermostability of the entire protein.
- 397:401 mutation Missing: No change in GlcNAc-1-P UTase activity. Shows 20% less GalN-1-P AcTase activity than the wild-type, but increases GlcN-1-P AcTase activity 4.8 times. Does not affect thermostability.
5z09A St0452(y97n)-utp binding form (see paper)
29% identity, 76% coverage: 6:260/335 of query aligns to 2:210/401 of 5z09A
- binding uridine 5'-triphosphate: L6 (≠ P10), A7 (≠ V11), A8 (= A12), G9 (= G13), S10 (≠ L14), G11 (= G15), E12 (≠ S16), R13 (= R17), Q73 (≠ R104), G79 (= G110), G98 (≠ A132), D99 (= D133)
Q88QT2 N-acetylmuramate alpha-1-phosphate uridylyltransferase; MurNAc-1P uridylyltransferase; MurNAc-alpha-1P uridylyltransferase; EC 2.7.7.99 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
25% identity, 76% coverage: 6:261/335 of query aligns to 2:208/223 of Q88QT2
- GER 11:13 (≠ GSR 15:17) binding
- K23 (= K27) binding
- N105 (≠ L131) binding
- D107 (= D133) binding
- D140 (≠ Y174) binding
- D205 (= D258) binding ; binding
4y7uA Structural analysis of muru (see paper)
25% identity, 76% coverage: 6:261/335 of query aligns to 2:208/224 of 4y7uA
- binding 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine: L6 (≠ P10), A7 (≠ V11), A8 (= A12), G9 (= G13), G11 (= G15), E12 (≠ S16), R13 (= R17), K23 (= K27), E84 (≠ G110), D107 (= D133)
- binding 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: N105 (≠ L131), D140 (≠ Y174), F160 (≠ V207), W203 (≠ R256), D205 (= D258)
- binding magnesium ion: D107 (= D133), D205 (= D258)
4ho4A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine and glucose-1-phosphate
27% identity, 71% coverage: 6:243/335 of query aligns to 2:206/289 of 4ho4A
Sites not aligning to the query:
4ho6A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-glucose and utp
27% identity, 71% coverage: 6:243/335 of query aligns to 2:206/288 of 4ho6A
- binding uridine-5'-diphosphate-glucose: L6 (≠ P10), G8 (≠ A12), G9 (= G13), Q80 (= Q105), P83 (= P108), G85 (= G110), D108 (= D133), G144 (= G175), E159 (= E194), K160 (= K195), V170 (= V207), R192 (≠ G229)
- binding uridine 5'-triphosphate: L43 (≠ E47), Y112 (≠ A140), G113 (= G141), H114 (≠ Q142), G115 (≠ K143), T117 (≠ V145)
Sites not aligning to the query:
Query Sequence
>HSERO_RS13375 FitnessBrowser__HerbieS:HSERO_RS13375
MKKITKAVFPVAGLGSRFLPATKAQPKEMLPIVDKPLIQYAVEEAVEAGITEMIFITGRN
KRAIEDHFDKAYELESELEAAGKVKLLETIRHVVPKHINCVYVRQAEPLGLGHAVLLAKP
LVGDEPFAVLLADDFMDVDAGQKNVMAQMTAMFEREGKSLLAVQDVPRPETRQYGIVSGS
AYADRLELMSGIVEKPAPEEAPSTLAVVGRYVLTPAIFERLENIGTGAGGEIQLTDAISA
LLQQEQILAYRYEGQRFDCGSKLGYLKAAVAMGFKHKEVGSGFAQFLQDFAAQHDLQQAA
QPKGPATVTPLKPNAAPAHGGAGQEKQPVAANSVM
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory