SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS15175 FitnessBrowser__HerbieS:HSERO_RS15175 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
40% identity, 80% coverage: 60:293/294 of query aligns to 140:384/409 of O53289

query
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O53289

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D185 (= D93) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ M94) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D95) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S96) mutation to A: No effect on enzymatic activity.
S273 (= S182) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K227) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D250) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D254) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

7qplA Crystal structure of phosphoserine phosphatase (serb) from brucella melitensis in complex with phosphate and magnesium
40% identity, 81% coverage: 54:292/294 of query aligns to 44:285/295 of 7qplA

query
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7qplA

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binding magnesium ion: D86 (= D93), D88 (= D95), D243 (= D250)

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
40% identity, 81% coverage: 57:293/294 of query aligns to 147:382/396 of 8a21A

query
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8a21A

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binding magnesium ion: D183 (= D93), D185 (= D95), D339 (= D250)
binding 4-phenyl-1h-imidazole: D185 (= D95), E192 (= E102), V193 (≠ C103), I194 (= I104), T211 (= T121), M215 (= M125), F221 (= F131), R228 (= R138), G273 (= G184)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
40% identity, 81% coverage: 57:293/294 of query aligns to 147:382/396 of 8a1zA

query
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8a1zA

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binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D95), E192 (= E102), M215 (= M125), F221 (= F131), L225 (= L135), R228 (= R138), G272 (= G183), F274 (= F185), D339 (= D250)
binding magnesium ion: D183 (= D93), D185 (= D95), D339 (= D250)

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
40% identity, 80% coverage: 59:293/294 of query aligns to 153:386/411 of A0QJI1

query
sites
A0QJI1

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R

N

A

A

K

K

P

P

V

A

V

L

R

R

A

E

Q

V

A

A

S

D

V

A

G

S

L

L

N

S

F

H

V

P

G

Y

L

L

D

D

G

T

I

V

L

L

N

F

L

L

D187 (= D93) binding
D189 (= D95) binding
D343 (= D250) binding

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
40% identity, 80% coverage: 59:293/294 of query aligns to 149:382/396 of 5jlpA

query
sites
5jlpA

A

A

L

L

K

R

A

T

S

A

V

L

D

N

A

R

A

V

C

S

L

S

Q

E

A

E

R

H

L

V

D

D

Y

V

A

A

F

V

I

E

D

D

A

Y

D

T

R

L

K

E

L

R

A

R

D

A

F

K

R

R

L

L

V

I

A

V

M

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

L

I

V

T

Q

I

G

E

E

C

V

I

I

D

E

E

M

I

L

A

A

D

A

M

K

Q

A

G

G

L

A

K

E

P

G

Q

Q

V

V

A

A

E

A

I

I

T

T

E

D

A

A

M

M

R

R

G

G

E

E

I

L

E

D

F

F

N

A

E

Q

S

S

L

L

T

Q

R

Q

R

R

V

V

A

A

L

T

L

L

K

A

G

G

L

L

D

P

A

A

G

T

A

V

L

I

Q

D

R

E

V

V

Y

A

D

G

E

Q

R

-

L

L

Q

E

L

L

S

M

P

P

G

G

A

A

E

R

N

T

M

T

L

L

K

R

A

T

V

L

Q

R

A

R

A

L

G

G

L

Y

K

A

T

C

L

G

L

V

V

V

S

S

G
|
G

G

G

F

F

T

R

F

R

F

I

T

I

D

E

R

P

M

L

K

A

A

E

R

E

L

L

N

M

L

L

D

D

Y

Y

T

V

H

A

A

A

N

N

T

E

L

L

E

E

V

I

V

V

D

D

G

G

K

T

L

L

T

T

G

G

K

R

V

V

G

G

G

P

I

I

V

I

N

D

A

R

D

A

E

G

K
|
K

R

A

A

T

T

A

V

L

E

R

R

E

V

F

C

A

R

Q

E

R

I

A

G

G

A

V

E

P

P

M

T

A

Q

Q

A

T

I

V

V

A

M

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

R

D

M

M

M

L

G

A

I

A

S

A

G

G

L

L

S

G

V

I

A

A

F

F

R

N

A

A

K

K

P

P

V

A

V

L

R

R

A

E

Q

V

A

A

S

D

V

A

G

S

L

L

N

S

F

H

V

P

G

Y

L

L

D

D

G

T

I

V

L

L

N

F

L

L

active site: D183 (= D93), V184 (≠ M94), D185 (= D95), G272 (= G183), K316 (= K227), D343 (= D254)
binding magnesium ion: D183 (= D93), D339 (= D250)

Sites not aligning to the query:

binding serine: 13, 14

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
37% identity, 70% coverage: 88:293/294 of query aligns to 5:209/210 of 1f5sA

query
sites
1f5sA

R

K

L

L

V

I

A

L

M

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

N

E

E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

M

E

Q

A

G

G

L

V

K

E

P

E

Q

E

V

V

A

K

E

K

I

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

I

L

E

N

F

F

N

E

E

Q

S

S

L

L

T

R

R

K

R

R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

G

E

A

K

L

V

Q

E

R

K

V

A

Y

I

D

-

E

K

R

R

L

I

Q

T

L

P

S

T

P

E

G

G

A

A

E

E

N

E

M

T

L

I

K

K

A

E

V

L

Q

K

A

N

A

R

G

G

L

Y

K

V

T

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

T

D

F

I

F

A

T

V

D

N

R

K

M

I

K

K

A

E

R

K

L

L

N

G

L

L

D

D

Y

Y

T

A

H

F

A

A

N

N

T

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

K

D

V

V

V

E

G

G

G

E

I

V

V

L

N

K

A

E

D

N

E

A

K
|
K

R

G

A

E

T

I

V

L

E

E

R

K

V

I

C

A

R

K

E

I

I

E

G

G

A

I

E

N

P

L

T

E

Q

D

A

T

I

V

V

A

M

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

R

S

M

M

M

F

G

K

I

K

S

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

G

C

L

I

N

E

F

K

V

R

G

D

L

L

D

R

G

E

I

I

L

L

N

K

L

Y

L

I

active site: D10 (= D93), F11 (≠ M94), D12 (= D95), G99 (= G183), K143 (= K227), D170 (= D254)
binding magnesium ion: D10 (= D93), D12 (= D95), D166 (= D250)
binding phosphate ion: D10 (= D93), F11 (≠ M94), D12 (= D95), S98 (= S182), G99 (= G183), K143 (= K227)

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
37% identity, 70% coverage: 88:293/294 of query aligns to 6:210/211 of Q58989

query
sites
Q58989

R

K

L

L

V

I

A

L

M

F

D
|
D

M

F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

N

E
|
E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

M

E

Q

A

G

G

L

V

K

E

P

E

Q

E

V

V

A

K

E

K

I

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

I

L

E

N

F

F

N

E

E

Q

S

S

L

L

T

R

R

K

R
|
R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

G

E

A

K

L

V

Q

E

R

K

V

A

Y

I

D

-

E

K

R

R

L

I

Q

T

L

P

S

T

P

E

G

G

A

A

E

E

N

E

M

T

L

I

K

K

A

E

V

L

Q

K

A

N

A

R

G

G

L

Y

K

V

T

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

T

D

F

I

F

A

T

V

D

N

R

K

M

I

K

K

A

E

R

K

L

L

N

G

L

L

D

D

Y

Y

T

A

H

F

A

A

N

N

T

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

K

D

V

V

V

E

G

G

G

E

I

V

V

L

N

K

A

E

D

N

E

A

K
|
K

R

G

A

E

T

I

V

L

E

E

R

K

V

I

C

A

R

K

E

I

I

E

G

G

A

I

E

N

P

L

T

E

Q

D

A

T

I

V

V

A

M

V

G

G

D
|
D

G

G

A

A

N
|
N

D

D

L

I

R

S

M

M

M

F

G

K

I

K

S

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

G

C

L

I

N

E

F

K

V

R

G

D

L

L

D

R

G

E

I

I

L

L

N

K

L

Y

L

I

D11 (= D93) active site, Nucleophile; binding ; mutation to N: Loss of activity.
D13 (= D95) active site, Proton donor; binding
E20 (= E102) binding
R56 (= R138) binding
SG 99:100 (= SG 182:183) binding
K144 (= K227) binding
D167 (= D250) binding
N170 (= N253) binding

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
37% identity, 70% coverage: 88:293/294 of query aligns to 4:208/209 of 1l7nA

query
sites
1l7nA

R

K

L

L

V

I

A

L

M

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

N

E

E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

M

E

Q

A

G

G

L

V

K

E

P

E

Q

E

V

V

A

K

E

K

I

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

I

L

E

N

F

F

N

E

E

Q

S

S

L

L

T

R

R

K

R

R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

G

E

A

K

L

V

Q

E

R

K

V

A

Y

I

D

-

E

K

R

R

L

I

Q

T

L

P

S

T

P

E

G

G

A

A

E

E

N

E

M

T

L

I

K

K

A

E

V

L

Q

K

A

N

A

R

G

G

L

Y

K

V

T

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

T

D

F

I

F

A

T

V

D

N

R

K

M

I

K

K

A

E

R

K

L

L

N

G

L

L

D

D

Y

Y

T

A

H

F

A

A

N

N

T

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

K

D

V

V

V

E

G

G

G

E

I

V

V

L

N

K

A

E

D

N

E

A

K
|
K

R

G

A

E

T

I

V

L

E

E

R

K

V

I

C

A

R

K

E

I

I

E

G

G

A

I

E

N

P

L

T

E

Q

D

A

T

I

V

V

A

M

V

G

G

D
|
D

G

G

A

A

N
|
N

D
|
D

L

I

R

S

M

M

M

F

G

K

I

K

S

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

G

C

L

I

N

E

F

K

V

R

G

D

L

L

D

R

G

E

I

I

L

L

N

K

L

Y

L

I

active site: D9 (= D93), F10 (≠ M94), D11 (= D95), G98 (= G183), K142 (= K227), D169 (= D254)
binding aluminum fluoride: D9 (= D93), F10 (≠ M94), D11 (= D95), S97 (= S182), K142 (= K227)
binding tetrafluoroaluminate ion: D9 (= D93), F10 (≠ M94), D11 (= D95), S97 (= S182), G98 (= G183), K142 (= K227), N168 (= N253)
binding magnesium ion: D9 (= D93), D11 (= D95), D165 (= D250)

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
37% identity, 70% coverage: 88:293/294 of query aligns to 3:207/208 of 1l7pA

query
sites
1l7pA

R

K

L

L

V

I

A

L

M

F

D
x
N

M
x
F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

N

E
|
E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

M

E

Q

A

G

G

L

V

K

E

P

E

Q

E

V

V

A

K

E

K

I

I

T

T

E

K

A

E

A

A

M
|
M

R

E

G

G

E

K

I

L

E

N

F
|
F

N

E

E

Q

S

S

L

L

T

R

R

K

R
|
R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

G

E

A

K

L

V

Q

E

R

K

V

A

Y

I

D

-

E

K

R

R

L

I

Q

T

L

P

S

T

P

E

G

G

A

A

E

E

N

E

M

T

L

I

K

K

A

E

V

L

Q

K

A

N

A

R

G

G

L

Y

K

V

T

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

T

D

F

I

F

A

T

V

D

N

R

K

M

I

K

K

A

E

R

K

L

L

N

G

L

L

D

D

Y

Y

T

A

H

F

A

A

N

N

T

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

K

D

V

V

V

E

G

G

G

E

I

V

V

L

N

K

A

E

D

N

E

A

K
|
K

R

G

A

E

T

I

V

L

E

E

R

K

V

I

C

A

R

K

E

I

I

E

G

G

A

I

E

N

P

L

T

E

Q

D

A

T

I

V

V

A

M

V

G

G

D

D

G

G

A

A

N

N

D
|
D

L

I

R

S

M

M

M

F

G

K

I

K

S

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

G

C

L

I

N

E

F

K

V

R

G

D

L

L

D

R

G

E

I

I

L

L

N

K

L

Y

L

I

active site: N8 (≠ D93), F9 (≠ M94), D10 (= D95), G97 (= G183), K141 (= K227), D168 (= D254)
binding phosphoserine: N8 (≠ D93), F9 (≠ M94), D10 (= D95), E17 (= E102), M40 (= M125), F46 (= F131), R53 (= R138), S96 (= S182), G97 (= G183), K141 (= K227)

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
35% identity, 70% coverage: 88:293/294 of query aligns to 3:199/200 of 1l7oA

query
sites
1l7oA

R

K

L

L

V

I

A

L

M

F

D

N

M

F

D

D

S

S

T

T

L

L

I

V

T

N

I

N

E

E

C

T

I

I

D

D

E

E

I

I

A

A

D

R

M

E

Q

A

G

G

L

V

K

E

P

E

Q

E

V

V

A

K

E

K

I

I

T

T

E

N

A

-

M

-

R

-

G

-

E

-

I

-

E

-

F

F

N

E

E

Q

S

S

L

L

T

R

R

K

R

R

V

V

A

S

L

L

K

K

G

D

L

L

D

P

A

I

G

E

A

K

L

V

Q

E

R

K

V

A

Y

I

D

-

E

K

R

R

L

I

Q

T

L

P

S

T

P

E

G

G

A

A

E

E

N

E

M

T

L

I

K

K

A

E

V

L

Q

K

A

N

A

R

G

G

L

Y

K

V

T

V

L

A

L

V

V

V

S

S

G

G

F

F

T

D

F

I

F

A

T

V

D

N

R

K

M

I

K

K

A

E

R

K

L

L

N

G

L

L

D

D

Y

Y

T

A

H

F

A

A

N

N

T

R

L

L

E

I

V

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

K

D

V

V

V

E

G

G

G

E

I

V

V

L

N

K

A

E

D

N

E

A

K

K

R

G

A

E

T

I

V

L

E

E

R

K

V

I

C

A

R

K

E

I

I

E

G

G

A

I

E

N

P

L

T

E

Q

D

A

T

I

V

V

A

M

V

G

G

D

D

G

G

A

A

N

N

D

D

L

I

R

S

M

M

M

F

G

K

I

K

S

A

G

G

L

L

S

K

V

I

A

A

F

F

R

C

A

A

K

K

P

P

V

I

V

L

R

K

A

E

Q

K

A

A

S

D

V

I

G
x
C

L

I

N
x
E

F

K

V

R

G

D

L

L

D

R

G

E

I

I

L

L

N

K

L

Y

L

I

binding zinc ion: C186 (≠ G280), E188 (≠ N282)

3m1yC Crystal structure of a phosphoserine phosphatase (serb) from helicobacter pylori
34% identity, 66% coverage: 88:282/294 of query aligns to 4:196/208 of 3m1yC

query
sites
3m1yC

R

K

L

L

V

A

A

V

M

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

T

N

I

A

E

E

C

T

I

I

D

E

E

S

I

L

A

A

D

R

M

A

Q

W

G

G

L

V

K

F

P

D

Q

E

V

V

A

K

E

T

I

I

T

T

E

L

A

K

A

A

M

M

R

N

G

G

E

-

I

T

E

D

F

F

N

H

E

K

S

S

L

L

T

I

R

L

R

R

V

V

A

S

L

K

L

L

K

K

G

N

L

M

D

P

A

L

G

K

A

L

L

A

Q

K

R

E

V

V

Y

C

D

-

E

E

R

S

L

L

Q

P

L

L

S

F

P

E

G

G

A

A

E

L

N

E

M

L

L

V

K

S

A

A

V

L

Q

K

A

E

A

K

G

N

L

Y

K

K

T

V

L

V

L

C

V

F

S

S

G
|
G

G

G

F

F

T

D

F

L

F

A

T

T

D

N

R

H

M

Y

K

R

A

D

R

L

L

L

N

H

L

L

D

D

Y

A

T

A

H

F

A

S

N

N

T

T

L

L

E

I

V

V

V

E

D

N

G

D

K

A

L

L

T

N

G

G

K

L

V

V

V

T

G

G

G

H

I

M

V

M

N

F

A

S

D

H

E

S

K
|
K

R

G

A

E

T

M

V

L

E

L

R

V

V

L

C

Q

R

R

E

L

I

L

G

N

A

I

E

S

P

K

T

T

Q

N

A

T

I

L

V

V

M

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

L

R

S

M

M

M

F

G

K

I

H

S

A

G

H

L

I

S

K

V

I

A

A

F

F

R

N

A

A

K

K

P

E

V

V

V

L

R

K

A

Q

Q

H

A

A

S

T

V

H

G

C

L

I

N

N

active site: D9 (= D93), F10 (≠ M94), D11 (= D95), G97 (= G183), K141 (= K227), D168 (= D254)
binding magnesium ion: D9 (= D93), D11 (= D95), D164 (= D250)

6hyjB Psph human phosphoserine phosphatase (see paper)
29% identity, 56% coverage: 90:255/294 of query aligns to 17:184/223 of 6hyjB

query
sites
6hyjB

V

V

A

C

M

F

D
|
D

M

V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E
|
E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

M

I

Q

C

G

G

L

V

K

E

P

D

Q

A

V

V

A

S

E

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

E

P

F

F

N

K

E

A

S

A

L

L

T

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

G

Q

A

V

L

Q

Q

R

R

L

V

I

Y

A

D

E

E

Q

R

P

L

P

Q

H

L

L

S

T

P

P

G

G

A

I

E

R

N

E

M

L

L

V

K

S

A

R

V

L

Q

Q

A

E

A

R

G

N

L

V

K

Q

T

V

L

F

L

L

V

I

S

S

G

G

F

F

T

R

F

S

F

I

T

V

D

E

R

H

M

V

K

A

A

S

R

K

L

L

N

N

L

I

D

P

Y

A

T

T

H

N

A

-

N

-

T

-

L

-

E

-

V

V

V
x
F

D

A

G

N

K

R

L

L

T

K

G

F

K

Y

V

F

V

N

G

G

G

E

I

Y

V

A

N

G

A

F

D

D

E

E

K

T

R

Q

A

P

T

T

V

A

E

E

-

S

-

G

-

K

-

G

-

K

-

V

-

I

R

K

V

L

C

L

R

K

E

E

I

-

G
x
K

A

F

E

H

P

F

T

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

binding calcium ion: D20 (= D93), D22 (= D95), D179 (= D250)
binding phosphoserine: F131 (≠ V209), K168 (≠ G239)
binding serine: E29 (= E102)

Sites not aligning to the query:

binding serine: 202

6q6jB Human phosphoserine phosphatase with substrate analogue homo-cysteic acid (see paper)
28% identity, 64% coverage: 90:276/294 of query aligns to 13:200/217 of 6q6jB

query
sites
6q6jB

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E

E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

M

I

Q

C

G

G

L

V

K

E

P

D

Q

A

V

V

A

S

E

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

E

P

F
|
F

N

K

E

A

S

A

L

L

T

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

G

Q

A

V

L

Q

Q

R

R

L

V

I

Y

A

D

E

E

Q

R

P

L

P

Q

H

L

L

S

T

P

P

G

G

A

I

E

R

N

E

M

L

L

V

K

S

A

R

V

L

Q

Q

A

E

A

R

G

N

L

V

K

Q

T

V

L

F

L

L

V

I

S
|
S

G
|
G

F

F

T

R

F

S

F

I

T

V

D

E

R

H

M

V

K

A

A

S

R

K

L

L

N

N

L

I

D

P

Y

A

T

T

H

N

A

-

N

-

T

-

L

-

E

-

V

V

V

F

D

A

G

N

K

R

L

L

T

K

G

F

K

Y

V

F

V

N

G

G

G

E

I

Y

V

A

N

G

A

F

D

D

E

E

K

T

R

Q

A

P

T

T

V

A

E

E

-

S

-

G

-
x
K

-

G

-

K

-

V

-

I

R

K

V

L

C

L

R

K

E

E

I

-

G

K

A

F

E

H

P

F

T

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

R

E

M

A

M

C

G

P

I

P

S

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R

R

A

Q

Q

Q

binding calcium ion: D16 (= D93), D18 (= D95), D175 (= D250)
binding (2~{S})-2-azanyl-4-sulfo-butanoic acid: D16 (= D93), V17 (≠ M94), D18 (= D95), F54 (= F131), S105 (= S182), G106 (= G183), G107 (= G184), K154 (vs. gap), T178 (≠ N253)

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
28% identity, 64% coverage: 90:276/294 of query aligns to 14:201/222 of 1l8oA

query
sites
1l8oA

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E

E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

M

I

Q

C

G

G

L

V

K

E

P

D

Q

A

V

V

A

S

E

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

E

P

F

F

N

K

E

A

S

A

L

L

T

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

G

Q

A

V

L

Q

Q

R

R

L

V

I

Y

A

D

E

E

Q

R

P

L

P

Q

H

L

L

S

T

P

P

G

G

A

I

E

R

N

E

M

L

L

V

K

S

A

R

V

L

Q

Q

A

E

A

R

G

N

L

V

K

Q

T

V

L

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

T

R

F

S

F

I

T

V

D

E

R

H

M

V

K

A

A

S

R

K

L

L

N

N

L

I

D

P

Y

A

T

T

H

N

A

-

N

-

T

-

L

-

E

-

V

V

V

F

D

A

G

N

K

R

L

L

T

K

G

F

K

Y

V

F

V

N

G

G

G

E

I

Y

V

A

N

G

A

F

D

D

E

E

K

T

R

Q

A

P

T

T

V

A

E

E

-

S

-

G

-
x
K

-

G

R

K

V

V

C

I

R

K

E

F

I

L

G

K

A

E

E

K

-

F

-

H

P

F

T

K

Q

K

A

I

I

I

V

M

M

I

G

G

D

D

G
|
G

A

A

N
x
T

D
|
D

L

M

R

E

M

A

M

C

G

P

I

P

S

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R
|
R

A

Q

Q

Q

active site: D17 (= D93), V18 (≠ M94), D19 (= D95), G107 (= G183), K155 (vs. gap), D180 (= D254)
binding phosphate ion: D17 (= D93), D19 (= D95), S106 (= S182), K155 (vs. gap)
binding serine: G177 (= G251), T179 (≠ N253), R199 (= R274)

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
28% identity, 64% coverage: 90:276/294 of query aligns to 14:201/222 of 1l8lA

query
sites
1l8lA

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E

E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

M

I

Q

C

G

G

L

V

K

E

P

D

Q

A

V

V

A

S

E

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

E

P

F

F

N

K

E

A

S

A

L

L

T

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

G

Q

A

V

L

Q

Q

R

R

L

V

I

Y

A

D

E

E

Q

R

P

L

P

Q

H

L

L

S

T

P

P

G

G

A

I

E

R

N

E

M

L

L

V

K

S

A

R

V

L

Q

Q

A

E

A

R

G

N

L

V

K

Q

T

V

L

F

L

L

V

I

S

S

G
|
G

G

G

F

F

T

R

F

S

F

I

T

V

D

E

R

H

M

V

K

A

A

S

R

K

L

L

N

N

L

I

D

P

Y

A

T

T

H

N

A

-

N

-

T

-

L

-

E

-

V

V

V

F

D

A

G

N

K

R

L

L

T

K

G

F

K

Y

V

F

V

N

G

G

G

E

I

Y

V

A

N

G

A

F

D

D

E

E

K

T

R

Q

A

P

T

T

V

A

E

E

-

S

-

G

-
x
K

-

G

R

K

V

V

C

I

R

K

E

F

I

L

G

K

A

E

E

K

-

F

-

H

P

F

T

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G
|
G

A

A

N
x
T

D
|
D

L

M

R

E

M

A

M

C

G

P

I

P

S

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R

R

A

Q

Q

Q

active site: D17 (= D93), V18 (≠ M94), D19 (= D95), G107 (= G183), K155 (vs. gap), D180 (= D254)
binding d-2-amino-3-phosphono-propionic acid: D17 (= D93), D19 (= D95), G107 (= G183), K155 (vs. gap), D176 (= D250), G177 (= G251), T179 (≠ N253)

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
29% identity, 56% coverage: 90:255/294 of query aligns to 17:184/225 of P78330

query
sites
P78330

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S
|
S

T

T

L

V

I

I

T

R

I

E

E
|
E

C

G

I

I

D
|
D

E

E

I

L

A
|
A

D

K

M

I

Q

C

G

G

L

V

K

E

P

D

Q

A

V

V

A

S

E

E

I

M

T

T

E

R

A

R

A

A

M
|
M

R
x
G

G

G

E

A

I

V

E

P

F

F

N

K

E

A

S

A

L

L

T

T

R

E

R
|
R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

G

Q

A

V

L

Q

Q

R

R

L

V

I

Y

A

D

E

E

Q

R

P

L

P

Q

H

L

L

S

T

P

P

G

G

A

I

E

R

N

E

M

L

L

V

K

S

A

R

V

L

Q

Q

A

E

A

R

G

N

L

V

K

Q

T

V

L

F

L

L

V

I

S
|
S

G
|
G

F

F

T

R

F

S

F

I

T

V

D

E

R

H

M

V

K

A

A

S

R

K

L

L

N

N

L

I

D

P

Y

A

T

T

H

N

A

-

N

-

T

-

L

-

E

-

V

V

V

F

D

A

G
x
N

K

R

L

L

T

K

G

F

K

Y

V

F

V

N

G

G

G

E

I

Y

V

A

N

G

A

F

D

D

E

E

K

T

R

Q

A

P

T

T

V

A

E

E

-

S

-

G

-
x
K

-

G

-

K

-

V

-

I

R

K

V

L

C

L

R

K

E

E

I

-

G

K

A

F

E

H

P

F

T

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

D20 (= D93) binding
DVD 20:22 (≠ DMD 93:95) binding
D22 (= D95) binding
S23 (= S96) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E102) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (= D105) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A108) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M125) binding ; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ R126) binding
R65 (= R138) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (= SGG 182:184) binding ; binding
N133 (≠ G211) mutation to A: Reduces L-phosphoserine phosphatase activity by about 75%.
K158 (vs. gap) binding ; binding
D179 (= D250) binding
T182 (≠ N253) binding ; binding ; mutation to S: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to V: Reduces L-phosphoserine phosphatase activity by about 25%.

Sites not aligning to the query:

202 R→A: Reduces L-phosphoserine phosphatase activity by about 99%.; R→K: Reduces L-phosphoserine phosphatase activity by about 95%.

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
28% identity, 64% coverage: 90:276/294 of query aligns to 13:200/221 of 6hyyA

query
sites
6hyyA

V

V

A

C

M

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

T

R

I

E

E

E

C

G

I

I

D

D

E

E

I

L

A

A

D

K

M

I

Q

C

G

G

L

V

K

E

P

D

Q

A

V

V

A

S

E

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

E

P

F

F

N

K

E

A

S

A

L

L

T

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

L

S

D

R

A

E

G

Q

A

V

L

Q

Q

R

R

L

V

I

Y

A

D

E

E

Q

R

P

L

P

Q

H

L

L

S

T

P

P

G

G

A

I

E

R

N

E

M

L

L

V

K

S

A

R

V

L

Q

Q

A

E

A

R

G

N

L

V

K

Q

T

V

L

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

T

R

F

S

F

I

T

V

D

E

R

H

M

V

K

A

A

S

R

K

L

L

N

N

L

I

D

P

Y

A

T

T

H

N

A

-

N

-

T

-

L

-

E

-

V

V

V

F

D

A

G

N

K

R

L

L

T

K

G

F

K

Y

V

F

V

N

G

G

G

E

I

Y

V

A

N

G

A

F

D

D

E

E

K

T

R

Q

A

P

T

T

V

A

E

E

-

S

-

G

-
x
K

-

G

-

K

-

V

-

I

R

K

V

L

C

L

R

K

E

E

I

-

G

K

A

F

E

H

P

F

T

K

Q

K

A

I

I

I

V

M

M

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

R

E

M

A

M

C

G

P

I

P

S

A

G

D

L

A

S

F

V

I

A

G

F

F

R

G

A

G

K

N

P

-

V

V

V

I

R

R

A

Q

Q

Q

binding magnesium ion: D16 (= D93), D18 (= D95), D175 (= D250)
binding serine: D16 (= D93), V17 (≠ M94), D18 (= D95), S105 (= S182), G106 (= G183), K154 (vs. gap)

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
32% identity, 44% coverage: 88:215/294 of query aligns to 10:133/200 of 4ap9A

query
sites
4ap9A

R

K

L

V

V

A

A

V

M

I

D
|
D

M
x
I

D
x
E

S

G

T

T

L

L

I

T

T

D

I

F

E

E

C

F

I

W

D

R

E

E

I

M

A

A

D
x
R

M
x
I

Q
x
T

G

G

L

-

K

K

P

R

Q

E

V

I

A

E

E

E

I

L

T

L

E

E

A

K

A

G

M
x
L

R

S

G

G

E

E

I

V

E

E

F
x
W

N

L

E

D

S

S

L

L

T

L

R

K

R

R

V

V

A

G

L

L

L

I

K

R

G

G

L

I

D

D

A

E

G

G

A

T

L

F

Q

L

R

R

V

T

Y

R

D

-

E

E

R

K

L

V

Q

N

L

V

S

S

P

P

G

E

A

A

E

R

N

E

M

L

L

V

K

E

A

T

V

L

Q

R

A

E

A

K

G

G

L

F

K

K

T

V

L

V

L

L

V

I

S

S

G
|
G

G

S

F

F

T

E

F

E

F

V

T

L

D

E

R

P

M

F

K

K

A

E

R

-

L

L

N

G

L

-

D

D

Y

E

T

F

H

M

A

A

N

N

T

R

L

A

E

I

V

F

V

E

D

D

G

G

K

K

L

F

T

Q

G

G

active site: D15 (= D93), I16 (≠ M94), E17 (≠ D95), G103 (= G183)
binding 3-pyridinium-1-ylpropane-1-sulfonate: R31 (≠ D109), I32 (≠ M110), T33 (≠ Q111), L46 (≠ M125), W52 (≠ F131)

Sites not aligning to the query:

active site: 141, 162
binding 3-pyridinium-1-ylpropane-1-sulfonate: 140, 141, 160, 161

6iuyA Structure of dsgpdh of dunaliella salina (see paper)
27% identity, 57% coverage: 90:258/294 of query aligns to 17:183/585 of 6iuyA

query
sites
6iuyA

V

V

A

C

M

F

D
|
D

M

V

D
|
D

S

R

T

T

L

V

I

T

T

T

I

D

E

A

C

S

I

V

D

G

E

L

I

L

A

A

D

K

M

F

Q

M

G

G

L

I

K

E

P

D

Q

E

V

A

A

Q

E

S

I

L

T

T

E

E

A

Q

A

A

M

N

R

R

G

G

E

E

I

I

E

N

F

L

N

T

E

K

S

A

L

F

T

E

R

D

R

R

V

L

A

A

L

K

L

L

K

N

G

-

L

F

D

T

A

P

G

T

A

D

L

I

Q

D

R

R

V

F

Y

L

D

E

E

E

-

H

-

P

-

A

R

H

L

T

Q

R

L

L

S

V

P

P

G

G

A

V

E

E

N

N

M

L

L

I

K

A

A

A

V

L

Q

K

A

A

A

R

G

G

L

V

K

E

T

V

L

F

L

L

V

I

S

S

G

G

F

F

T

R

F

E

F

M

T

A

D

L

R

P

M

I

K

A

A

S

R

H

L

L

N

K

L

I

D

P

-

A

-

K

-

N

-

V

Y

F

T

C

H

N

A

T

N

M

T

S

L

W

E

Q

V

L

V

D

D

D

G

H

K

G

L

E

T

P

G

V

K

R

V

L

V

-

G

-

I

-

V

-

N

-

A

S

D

H

E

F

K

K

R

S

A

R

T

A

V

I

E

E

R

R

V

I

C

R

R

R

E

K

I

Y

G

-

A

-

E

-

P

P

T

Y

Q

N

A

N

I

I

V

I

M

M

-

V

G

G

D
|
D

G

G

A

F

N

S

D

D

L

L

R

E

M

A

M

M

binding magnesium ion: D20 (= D93), D22 (= D95), D175 (= D250)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 233, 234, 235, 236, 268, 290, 321, 324, 349, 381, 382, 497, 529, 530, 532

Query Sequence

>HSERO_RS15175 FitnessBrowser__HerbieS:HSERO_RS15175
MNQQNNMNLILQAPKQHAVSSSAIEAIAALAGGDVERISDARVPDADVWRVAGVSLNEAL
KASVDAACLQARLDYAFIDADRKLADFRLVAMDMDSTLITIECIDEIADMQGLKPQVAEI
TEAAMRGEIEFNESLTRRVALLKGLDAGALQRVYDERLQLSPGAENMLKAVQAAGLKTLL
VSGGFTFFTDRMKARLNLDYTHANTLEVVDGKLTGKVVGGIVNADEKRATVERVCREIGA
EPTQAIVMGDGANDLRMMGISGLSVAFRAKPVVRAQASVGLNFVGLDGILNLLA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory