SitesBLAST
Comparing HSERO_RS16495 FitnessBrowser__HerbieS:HSERO_RS16495 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5bntC X-ray crystal structure of a aspartate-semialdehyde dehydrogenase bound to NADP from pseudomonas aeruginosa
70% identity, 99% coverage: 1:366/370 of query aligns to 2:369/371 of 5bntC
- active site: C136 (= C135), Q163 (= Q162), R269 (= R266), H276 (= H273)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G8), R11 (= R10), G12 (= G11), M13 (= M12), V14 (= V13), T38 (= T37), S39 (= S38), Q74 (= Q73)
7tcmA Crystal structure of aspartate-semialdehyde dehydrogenase from acinetobacter baumannii in complex with NADP
68% identity, 98% coverage: 4:366/370 of query aligns to 5:369/373 of 7tcmA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G8), R11 (= R10), G12 (= G11), M13 (= M12), V14 (= V13), T38 (= T37), S39 (= S38), C74 (≠ A72), Q75 (= Q73), G76 (= G74), A100 (= A98), S167 (= S165), Q352 (= Q349), A357 (= A354)
1gl3A Aspartate beta-semialdehyde dehydrogenase in complex with NADP and substrate analogue s-methyl cysteine sulfoxide (see paper)
67% identity, 98% coverage: 1:363/370 of query aligns to 1:364/367 of 1gl3A
- active site: C135 (= C135), Q162 (= Q162), R267 (= R266), H274 (= H273)
- binding cysteine: C135 (= C135), Q162 (= Q162), I230 (= I229), E241 (= E240), R267 (= R266), H274 (= H273)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R10 (= R10), G11 (= G11), M12 (= M12), V13 (= V13), T37 (= T37), S38 (= S38), C72 (≠ A72), Q73 (= Q73), A98 (= A98), C135 (= C135), S165 (= S165), G166 (= G166), G168 (= G168), A169 (= A169), Q350 (= Q349), A355 (= A354)
P0A9Q9 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Escherichia coli (strain K12) (see 2 papers)
67% identity, 98% coverage: 1:363/370 of query aligns to 1:364/367 of P0A9Q9
- RGMV 10:13 (= RGMV 10:13) binding
- TS 37:38 (= TS 37:38) binding
- Q73 (= Q73) binding
- C135 (= C135) active site, Acyl-thioester intermediate; mutation to A: Complete loss of activity.; mutation to S: 99.7% loss of activity.
- SGGGA 165:169 (= SGGGA 165:169) binding
- R173 (= R173) binding
- P193 (= P193) binding
- Q350 (= Q349) binding
P44801 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see 2 papers)
65% identity, 98% coverage: 1:363/370 of query aligns to 1:367/371 of P44801
- RGMV 10:13 (= RGMV 10:13) binding
- TS 37:38 (= TS 37:38) binding
- Q74 (= Q73) binding
- R103 (= R102) binding ; mutation to K: 2-fold increase in affinity for ASA, 23-fold decrease in affinity for phosphate, and 275-fold decrease in activity.; mutation to L: 7-fold increase in affinity for ASA, 150-fold decrease in affinity for phosphate, and 1400-fold decrease in activity.
- C136 (= C135) active site, Acyl-thioester intermediate
- S166 (= S165) binding
- E243 (= E240) mutation to D: No change in affinity for ASA and 82-fold decrease in activity.
- K246 (= K243) binding ; mutation to R: 2-fold increase in affinity for ASA, nearly no change in affinity for phosphate, and 30-fold decrease in activity.
- R270 (= R266) mutation to K: 2-fold decrease in affinity for ASA and 825-fold decrease in activity.
- H277 (= H273) active site, Proton acceptor
- Q353 (= Q349) binding
1pquA Crystal structure of the h277n mutant of aspartate semialdehyde dehydrogenase from haemophilus influenzae bound with NADP, s-methyl cysteine sulfoxide and cacodylate (see paper)
65% identity, 98% coverage: 1:363/370 of query aligns to 1:367/371 of 1pquA
- active site: C136 (= C135), Q163 (= Q162), R270 (= R266), N277 (≠ H273)
- binding cysteine: C136 (= C135), Q163 (= Q162), G167 (= G166), E243 (= E240), R270 (= R266)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), R10 (= R10), G11 (= G11), M12 (= M12), V13 (= V13), T36 (≠ S36), T37 (= T37), S38 (= S38), C73 (≠ A72), Q74 (= Q73), A99 (= A98), S166 (= S165), L354 (= L350), A358 (= A354)
1tb4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound periodate (see paper)
64% identity, 98% coverage: 1:363/370 of query aligns to 1:353/357 of 1tb4A
1ta4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound arsenate (see paper)
64% identity, 98% coverage: 1:363/370 of query aligns to 1:353/357 of 1ta4A
1nx6A Crystal structure of aspartate semialdehyde dehydrogenase from haemophilus influenzae as a tetrahedral hemithiocetal reaction intermediate with phosphate at 2.15 a (see paper)
64% identity, 98% coverage: 1:363/370 of query aligns to 1:353/357 of 1nx6A
1pqpA Crystal structure of the c136s mutant of aspartate semialdehyde dehydrogenase from haemophilus influenzae bound with aspartate semialdehyde and phosphate (see paper)
63% identity, 98% coverage: 1:363/370 of query aligns to 1:353/357 of 1pqpA
- active site: S122 (≠ C135), Q149 (= Q162), R256 (= R266), H263 (= H273)
- binding l-homoserine: S122 (≠ C135), Q149 (= Q162), G153 (= G166), R256 (= R266), H263 (= H273), Q339 (= Q349)
- binding phosphate ion: R89 (= R102), N121 (= N134), S122 (≠ C135), K232 (= K243)
4r5mA Crystal structure of vc-aspartate beta-semialdehyde-dehydrogenase with NADP and 4-nitro-2-phosphono-benzoic acid (see paper)
63% identity, 98% coverage: 4:366/370 of query aligns to 3:367/369 of 4r5mA
- active site: C134 (= C135), Q161 (= Q162), R267 (= R266), H274 (= H273)
- binding 4-nitro-2-phosphonobenzoic acid: C71 (≠ A72), G73 (= G74), G74 (= G75), A96 (= A97), A97 (= A98), S98 (= S99), R101 (= R102), K243 (= K243)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G8), R9 (= R10), G10 (= G11), M11 (= M12), V12 (= V13), T36 (= T37), S37 (= S38), Q72 (= Q73), G73 (= G74), G165 (= G166)
1mb4A Crystal structure of aspartate semialdehyde dehydrogenase from vibrio cholerae with NADP and s-methyl-l-cysteine sulfoxide (see paper)
63% identity, 98% coverage: 4:366/370 of query aligns to 3:367/369 of 1mb4A
- active site: C134 (= C135), Q161 (= Q162), R267 (= R266), H274 (= H273)
- binding cysteine: N133 (= N134), C134 (= C135), G165 (= G166), E240 (= E240), R267 (= R266), H274 (= H273)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G8), R9 (= R10), G10 (= G11), M11 (= M12), V12 (= V13), T36 (= T37), S37 (= S38), C71 (≠ A72), Q72 (= Q73), G73 (= G74), A97 (= A98), S164 (= S165), Q350 (= Q349), L351 (= L350), A355 (= A354)
3pzrA Crystals structure of aspartate beta-semialdehyde dehydrogenase from vibrio cholerae with NADP and product of s-carbamoyl-l-cysteine (see paper)
63% identity, 98% coverage: 4:366/370 of query aligns to 3:367/370 of 3pzrA
- active site: C134 (= C135), Q161 (= Q162), R267 (= R266), H274 (= H273)
- binding cysteine: C134 (= C135), Q161 (= Q162), G165 (= G166), E240 (= E240), R267 (= R266), H274 (= H273), Q350 (= Q349)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G8), R9 (= R10), G10 (= G11), M11 (= M12), V12 (= V13), S35 (= S36), T36 (= T37), S37 (= S38)
Q9KQG2 Aspartate-semialdehyde dehydrogenase 1; ASA dehydrogenase 1; ASADH 1; Aspartate-beta-semialdehyde dehydrogenase 1; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
63% identity, 98% coverage: 4:366/370 of query aligns to 3:367/370 of Q9KQG2
3tz6A Crystal structure of aspartate semialdehyde dehydrogenase complexed with inhibitor smcs (cys) and phosphate from mycobacterium tuberculosis h37rv (see paper)
29% identity, 95% coverage: 4:355/370 of query aligns to 4:330/342 of 3tz6A
- active site: C129 (= C135), Q156 (= Q162), R248 (= R266), H255 (= H273)
- binding cysteine: C129 (= C135), Q156 (= Q162), G160 (= G166), E223 (= E240), R248 (= R266), H255 (= H273)
- binding glycerol: S108 (≠ D112), G187 (≠ S194), F192 (≠ D211), P201 (= P220), Q225 (≠ W242), R228 (≠ Q245), F229 (≠ A246)
- binding sulfate ion: R98 (= R102), H117 (≠ L123), R119 (≠ K125), N128 (= N134), C129 (= C135), K226 (= K243), E270 (≠ A288), R273 (≠ E291)
Sites not aligning to the query:
3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
25% identity, 95% coverage: 4:355/370 of query aligns to 4:330/358 of 3q11A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (≠ R10), G11 (= G11), A35 (≠ S36), S36 (≠ T37), R38 (≠ N39), S39 (≠ A40), T56 (≠ A57), A71 (≠ Q73), T75 (≠ Y77), G160 (= G168), M161 (= M179), G162 (= G180)
- binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R102), N126 (= N134), C127 (= C135), Q154 (= Q162), G158 (= G166), E219 (= E240), K222 (= K243), R244 (= R266)
4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
25% identity, 95% coverage: 4:355/370 of query aligns to 4:330/357 of 4r54A
- binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G74), S73 (≠ G75), T94 (≠ A98), S95 (= S99), R98 (= R102), K222 (= K243)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (≠ R10), G11 (= G11), A12 (≠ M12), V13 (= V13), A35 (≠ S36), S36 (≠ T37), R38 (≠ N39), S39 (≠ A40), T56 (≠ A57), S70 (≠ A72), A71 (≠ Q73), G72 (= G74), T75 (≠ Y77), N93 (≠ A97), T94 (≠ A98), N126 (= N134), C127 (= C135), G160 (= G168), G328 (= G353)
4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
25% identity, 95% coverage: 4:355/370 of query aligns to 4:330/357 of 4r41A
- binding 4-nitro-2-phosphonobenzoic acid: S70 (≠ A72), G72 (= G74), S73 (≠ G75), N93 (≠ A97), T94 (≠ A98), S95 (= S99), R98 (= R102), K222 (= K243)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (≠ R10), G11 (= G11), A12 (≠ M12), A35 (≠ S36), S36 (≠ T37), R38 (≠ N39), S39 (≠ A40), T56 (≠ A57), A71 (≠ Q73), G160 (= G168), M161 (= M179), G162 (= G180)
4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
25% identity, 95% coverage: 4:355/370 of query aligns to 4:330/357 of 4r3nA
- active site: C127 (= C135), Q154 (= Q162), R244 (= R266), H251 (= H273)
- binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G75), T94 (≠ A98), S95 (= S99), R98 (= R102), N126 (= N134), K222 (= K243)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (≠ R10), G11 (= G11), A12 (≠ M12), V13 (= V13), A35 (≠ S36), S36 (≠ T37), S39 (≠ A40), T56 (≠ A57), S70 (≠ A72), A71 (≠ Q73), G72 (= G74), N93 (≠ A97), T94 (≠ A98), N126 (= N134), C127 (= C135), G160 (= G168), M161 (= M179), G328 (= G353)
3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
25% identity, 95% coverage: 4:355/370 of query aligns to 4:330/357 of 3q1lA
Query Sequence
>HSERO_RS16495 FitnessBrowser__HerbieS:HSERO_RS16495
MKLVGLVGWRGMVGSVLMQRMQDEGDFDHIEPVFFSTSNAGGKAPSFAKKETTLKDANSI
DELKKCDIIITAQGGDYTNEIFPKLRAAGWDGYWIDAASSLRMKDDAVIVLDPVNNNVIK
DALAKGVKNYIGGNCTNSILLMGVGGLFKEGLVEWVSSMTYQAASGGGANHMRELLKGMG
VINAAVADELATPSSAILDIDRKVAKTIREDVPTEFFGAPLAGGLIPWIDAQLENGQSKE
EWKGQAEVNKILGNEQIIPVDGLCVRIGAMRCHSLALTIKLKRDLPLAEIESIIRAGNQW
VKWVPNDRAITVKELTPASITGGLEIGVGRVRKLNQGPEYISAFVIGDQLLWGAAEPLRR
MLRIILGNLA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory