SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS16735 FitnessBrowser__HerbieS:HSERO_RS16735 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
51% identity, 96% coverage: 1:425/444 of query aligns to 11:434/478 of Q47945

query
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Q47945

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Q37 (= Q27) modified: Pyrrolidone carboxylic acid

Sites not aligning to the query:

1:36 signal peptide

8gy2B Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
53% identity, 88% coverage: 35:425/444 of query aligns to 1:396/433 of 8gy2B

query
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8gy2B

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binding heme c: C18 (= C52), C21 (= C55), H22 (= H56), T46 (≠ S80), I48 (= I82), Y59 (= Y93), L68 (= L102), R73 (= R107), V79 (≠ L113), Y80 (= Y114), M83 (= M117), F88 (≠ Y122), R126 (= R160), H165 (= H197), C166 (= C198), C169 (= C201), H170 (= H202), I201 (≠ L231), A202 (= A232), P203 (≠ N233), L205 (= L235), W216 (= W246), F224 (= F254), A234 (= A264), V235 (≠ A265), F236 (= F266), F236 (= F266), M239 (= M269), N301 (= N331), C302 (= C332), C305 (= C335), H306 (= H336), M316 (≠ T346), F317 (= F347), P318 (= P348), L320 (= L350), P324 (= P354), G342 (≠ A372), S352 (≠ T382), V354 (≠ F384), M356 (= M386), F359 (= F389), M375 (≠ I405)
binding ubiquinone-10: C21 (= C55), L34 (= L68), P39 (= P73), P81 (= P115), L129 (= L163), W132 (= W166), E168 (≠ A200), R173 (= R205), I197 (= I227), D241 (= D271)

Sites not aligning to the query:

binding heme c: 428

7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
39% identity, 90% coverage: 40:437/444 of query aligns to 1:401/418 of 7w2jC

query
sites
7w2jC

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L

-

V

S

Q

R

P

L

L

S

T

D

D

Q

E

E

Q

V

I

A

A

E

T

V

I

V

A

T

D

F

Y

I

V

R

L

S

S

S

H

W

F

G

G

N

N

Q

A

A

Q

S

A

S

T

V

V

S

S

A

A

S

D

D

A

V

V

A

K

K

Q

V

V

R

R

-

A

-

G

K

K

Q

Q

L

V

P

P

E

L

K

A

K

K

T

L

V

A

E

S

A

P

G

G

binding heme c: C13 (= C52), C16 (= C55), H17 (= H56), T42 (≠ S80), I44 (= I82), Y55 (= Y93), L75 (= L113), Y76 (= Y114), A78 (= A116), M79 (= M117), R122 (= R160), H161 (= H197), C162 (= C198), C165 (= C201), H166 (= H202), A191 (= A232), P192 (≠ N233), R223 (≠ A264), P227 (≠ G268), M228 (= M269), V289 (≠ N331), C290 (= C332), C293 (= C335), H294 (= H336), Y305 (≠ T346), Y306 (≠ F347), P307 (= P348), L309 (= L350), N312 (≠ S353), T313 (≠ P354), T314 (= T355), D322 (≠ S363), I327 (≠ V368), V331 (≠ A372), R333 (≠ M374), I340 (≠ F384), M342 (= M386), P343 (= P387), F345 (= F389)

8jejC Cryo-em structure of na-dithionite reduced membrane-bound fructose dehydrogenase from gluconobacter japonicus
39% identity, 89% coverage: 40:432/444 of query aligns to 1:410/413 of 8jejC

query
sites
8jejC

V

I

Q

S

R

R

G

G

E

H

Y

Y

L

L

A

A

K

I

A

A

G

A

D

D

C
|
C

V

A

A

A

C
|
C

H
|
H

T

T

-

N

A

G

K

R

G

D

G

G

K

Q

P

F

F

L

A

A

G

G

L

Y

A

A

I

I

A

S

T

S

P

P

I
x
M

G

G

T

N

V

I

Y

Y

S

S

S
x
T

N

N

I
|
I

T

T

P

P

D

S

K

K

E

T

N

H

G

G

I

I

G

G

N

N

Y

Y

S

T

E

L

E

E

D

Q

F
|
F

D

S

R

K

A

A

L
|
L

R

R

H

H

G

G

I

I

R

R

K

A

D

D

G

G

A

A

S

Q

L
|
L

Y
|
Y

P
|
P

A

A

M
|
M

P
|
P

Y

Y

P

D

S

A

Y
|
Y

A

N

K

R

V

L

K

T

P

D

A

E

D

D

V

V

K

K

A

S

L

L

Y

Y

A

A

Y

Y

F

I

M

M

H

T

G

E

V

V

Q

K

A

P

D

V

P

D

A

A

P

P

N

S

R

P

G

K

V

T

D

Q

I

L

T

P

W

F

P

P

L

F

S

S

M

I

R
|
R

W

A

P
x
S

L

L

S

G

I

I

W
|
W

R

K

-

I

-

A

K

R

V

I

F

E

A

G

P

K

A

P

V

Y

A

V

V

F

D

D

G

H

P

T

E

H

D

-

N

N

S

D

P

D

L

W

V

N

R

R

G

G

Q

R

Y

Y

L

L

V

V

E

D

G

E

L

L

G

A

H

H

C
|
C

G

G

A

E

C
|
C

H
|
H

T

T

P

P

R

R

G

N

V

F

G

-

M

-

Q

-

E

-

K

-

A

L

L
|
L

S

A

N

P

D

N

S

Q

S

S

Q

A

F

Y

L

L

S

A

G

G

G

A

V

D

I
|
I

D

G

G

S

Y
x
W

L

R

A
|
A

N
x
P

N

N

L
x
I

R

T

G

N

D

A

G

P

R

Q

D

S

G

G

L

I

G

G

N

S

W
|
W

S

S

E

D

A

Q

D

D

I

L

V

F

A

Q

F
x
Y

L

L

K

K

T

T

G

G

R

K

N

T

S

A

H

H

S

A

A
x
R

A

A

F

A

G

G

G

P

M
|
M

A

A

D

E

V

A

V

I

A

E

N

H

S

S

T

L

Q

Q

Y

Y

M

L

T

P

E

D

E

A

D

D

L

I

S

S

A

A

M

I

A

V

K

T

Y

Y

L

L

K

R

S

S

L

V

K

-

P

P

V

A

K

K

-

A

-

E

-

S

-

G

-

Q

-

T

-

V

-

A

-

N

-

F

-

E

-

H

D

A

G

G

T

R

P

P

-

S

-

Y

-

S

-

V

A

A

L

N

A

A

Y

N

D

S

D

R

K

R

T

S

H

N

Q

S

A

T

L

L

R

T

K

K

G

T

S

T

D

D

Q

-

S

-

P

-

G

G

A

A

M

A

A

L

F

Y

L

E

N

A

N
x
V

C
|
C

A

A

A

S

C
|
C

H
|
H

R

Q

S

S

S

D

G

G

K

K

G

G

-

S

Y

K

D

D

E

G

T

Y

F
x
Y

P
|
P

S

S

L
|
L

A

V

L

G

S

N

P
x
T

T
|
T

V

T

N

G

A

Q

E

L

N

N

P

P

A

N

S
x
D

L

L

I

I

R

A

I

S

V
x
I

L

L

E

Y

G

G

A
x
V

E

D

M
x
R

P

T

W

T

T

D

H

N

K

H

A

-

P

-

T

-

Q

E

F
x
I

A

L

M
|
M

P

P

A

A

F
|
F

G

G

-

P

-

D

-

S

-

L

-

V

S

Q

R

P

L

L

S

T

D

D

Q

E

E

Q

V

I

A

A

E

T

V
x
I

V

A

T

D

F

Y

I

V

R

L

S

S

S

H

W

F

G

G

N

N

Q

A

A

Q

S

A

S

T

V

V

S

S

A

A

S

D

D

A

V

V

A

K

K

Q

V

V

R

R

-

A

-

G

K

K

Q

Q

L

V

P

P

E
x
L

K

A

K

K

binding heme c: C13 (= C52), C16 (= C55), H17 (= H56), T42 (≠ S80), I44 (= I82), F60 (= F98), L64 (= L102), L75 (= L113), Y76 (= Y114), M79 (= M117), P80 (= P118), Y84 (= Y122), R122 (= R160), C162 (= C198), C165 (= C201), H166 (= H202), I186 (= I227), W189 (≠ Y230), A191 (= A232), P192 (≠ N233), I194 (≠ L235), W205 (= W246), Y213 (≠ F254), R223 (≠ A264), M228 (= M269), V303 (≠ N331), C304 (= C332), C307 (= C335), H308 (= H336), Y320 (≠ F347), P321 (= P348), L323 (= L350), T327 (≠ P354), T328 (= T355), D336 (≠ S363), I341 (≠ V368), V345 (≠ A372), R347 (≠ M374), I354 (≠ F384), M356 (= M386), F359 (= F389), I376 (≠ V401)
binding ubiquinone-10: M36 (≠ I74), P77 (= P115), S124 (≠ P162), W128 (= W166), C165 (= C201), L173 (= L214), L408 (≠ E430)

Sites not aligning to the query:

binding ubiquinone-10: 411

8gy3A Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
34% identity, 85% coverage: 30:408/444 of query aligns to 31:410/440 of 8gy3A

query
sites
8gy3A

P

P

A

A

P

S

S

S

A

F

D

S

Q

A

Q

D

L

A

V

I

Q

S

R

R

G

G

E

E

Y

I

L

V

A

A

K

N

A

G

G

G

D
x
Y

C
|
C

V

A

A
x
E

C
|
C

H
|
H

T

T

A

R

K

V

G

D

G

G

K

K

P

P

-

G

-

P

-

E

F

L

A

A

G

G

G

D

L

F

A

K

I

M

A

A

T
|
T

P

P

I
x
F

G

G

T

D

V

I

Y

F

S

S

S
|
S

N

N

I
|
I

T

T

P

P

D

D

K

E

E

E

N

W

G

G

I

I

G

G

N

N

Y
x
W

S

S

E

L

E

A

D

A

F
|
F

D

K

R

R

A

A

L

M

R

N

H

K

G

G

I

I

R

A

K

R

D

D

G

G

A

S

S

Q

L
|
L

Y
|
Y

P
|
P

A

A

M
x
F

P

P

Y

F

P

D

S

H

Y
x
F

A

T

K

K

V

V

K

S

P

D

A

Q

D

D

V

V

K

S

A

D

L

L

Y

Y

A

A

Y

Y

F

L

M

M

H

T

G

R

V

P

Q

A

A

V

D

H

P

L

A

K

P

P

N

-

R

R

G

D

V

N

D

T

I

V

T

P

W

F

P

P

L
x
I

S

N

M

I

R
|
R

W

L

P

I

-
x
G

L
x
Q

S

G

I
x
F

W
|
W

R

K

K

L

V

L

-

F

F

F

A

T

P

P

A

G

V

R

A

Y

V

Q

D

N

G

D

P

P

E

K

D

H

N

D

S

A

P

Q

L

W

V

N

R

R

G

G

Q

A

Y

Y

L

L

V

A

E

E

G

G

L

N

G

E

H
|
H

C
|
C

G

G

A

A

C
|
C

H
|
H

T

T

P

P

R
|
R

G

N

V

L

G
x
L

M

G

Q

A

E

E

K

K

A

M

L

-

S

-

N

-

D

-

S

-

S

S

Q

S

F

V

L

Y

S

D

G

G

G

A

V

V

I

I

D

D

G

G

Y

W

L

I

A
|
A

N
x
P

N

P

L
|
L

R

N

G

-

D

D

G

H

R

N

D

P

G

T

L

P

G

V

N

V

W
|
W

S

T

E

E

A

D

D

E

I

L

V

F

A

Q

F
x
Y

L
|
L

K

R

T

F

G

G

R

V

N

A

S

P

-

L

H

H

S
x
G

A
x
S

A

A

F

A

G

G

G

P

M
|
M

A

S

D

P

V

V

V

P

A

H

N

R

S

F

T

L

Q

S

Y

K

M

I

T

P

E

E

D

D

L

V

S

H

A

A

M

I

A

A

K

H

Y

Y

L
x
Y

K

A

S

D

L

V

K

D

P

K

V

A

-

A

-

Q

K

R

D

S

G

S

T

G

P

D

A

Q

L

A

A

A

Y

I

D

T

D

R

K

A

T

M

H

Q

Q

M

A

S

L

G

R

R

K

D

G

L

S

T

D

G

Q

P

S

Q

P

P

-

L

-

D

-

E

G

D

A

A

M

R

A

L

F

Y

L

Q

N

G

N

A

C
|
C

A

G

A

A

C
|
C

H
|
H

R

Y

S

N

S

S

G

G

K

P

G

N

Y

P

D

V

E

L

T

G

F

R

P
|
P

S

E

L
|
L

A

A

L

L

S
x
N

P
x
N

T

A

V

L

N

W

A

L

E

D

N

E

P

P

A

N

S

N

L

L

I

Y

R

Q

I
x
V

V

M

L

L

E

H

G

G

A
x
I

E

T

M

-

P

-

W

-

T

A

H

E

K

E

A
x
G

P
x
Q

T

D

Q

H

F
x
I

A

S

M
|
M

P

P

A

S

F
|
F

G

Y

S

S

R

G

L

L

S

S

D

D

Q

H

E

D

V

M

A

A

E

R

V

I

V

A

T

A

F

Y

I

L

R

R

S

R

S

T

binding heme c: Y52 (≠ D51), C53 (= C52), C56 (= C55), H57 (= H56), S84 (= S80), I86 (= I82), W97 (≠ Y93), F102 (= F98), L117 (= L113), F121 (≠ M117), F126 (≠ Y122), R163 (= R160), C203 (= C198), C206 (= C201), H207 (= H202), A232 (= A232), P233 (≠ N233), L235 (= L235), W245 (= W246), Y253 (≠ F254), L254 (= L255), G263 (≠ S263), S264 (≠ A264), M269 (= M269), Y292 (≠ L292), C337 (= C332), C340 (= C335), H341 (= H336), P353 (= P348), L355 (= L350), N358 (≠ S353), N359 (≠ P354), V372 (≠ I367), I377 (≠ A372), G382 (≠ A380), Q383 (≠ P381), I386 (≠ F384), M388 (= M386), F391 (= F389)
binding ubiquinone-10: E55 (≠ A54), T76 (= T72), F78 (≠ I74), Y118 (= Y114), P119 (= P115), I160 (≠ L157), G166 (vs. gap), Q167 (≠ L163), F169 (≠ I165), W170 (= W166), H202 (= H197), R210 (= R205), L213 (≠ G208)

8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase (see paper)
37% identity, 23% coverage: 325:425/444 of query aligns to 23:121/121 of 8hddB

query
sites
8hddB

A

A

M

R

A

L

F

Y

L

L

N

G

N

N

C
|
C

A

A

A

T

C
|
C

H
|
H

R

Q

S

M

S

Q

G

G

K

K

G

G

Y

T

-

P

D

D

E

G

T

Y

F
x
Y

P
|
P

S

S

L

L

A

F

L

H

S

N

P

S

T
|
T

V

V

N

G

A

A

E

S

N

N

P

P

A

S

S

N

L

L

I

V

R

Q

I
x
V

V
x
I

L

L

E

N

G

G

A

V

E

Q

M
x
R

P

K

W

I

T

G

H

S

K

E

A

-

P

-

T

-

Q

D

F
x
I

A

G

M
|
M

P
|
P

A

A

F

F

G

R

S

Y

R

D

L

L

S

N

D

D

Q

A

E

Q

V

I

A

A

E

A

V

L

V

T

T

N

F

Y

I

V

R

T

S

A

S

Q

W

F

G

G

N

N

Q

P

A

A

S

A

S

K

V

V

S

T

A

E

S

Q

D

D

V

V

A

A

K

K

V

L

R

R

binding protoporphyrin ix containing fe: C30 (= C332), C33 (= C335), H34 (= H336), Y46 (≠ F347), P47 (= P348), T54 (= T355), V66 (≠ I367), I67 (≠ V368), R73 (≠ M374), I80 (≠ F384), M82 (= M386), P83 (= P387)

2zooA Crystal structure of nitrite reductase from pseudoalteromonas haloplanktis tac125
35% identity, 23% coverage: 324:425/444 of query aligns to 339:438/438 of 2zooA

query
sites
2zooA

G

G

A

Q

M

R

A

V

F

Y

L

E

N

A

N

N

C
|
C

A

M

A

A

C
|
C

H
|
H

R

Q

S

A

S

N

G

G

K

E

G

G

Y

I

D

P

E

G

T

A

F
|
F

P
|
P

S
x
P

L
|
L

A

A

L

K

S
|
S

P

D

T
x
Y

V

L

N

N

A

-

E

N

N

N

P

P

A

L

S

L

L

G

I

V

R

N

I

A

V
x
I

L

I

E

K

G

G

A
x
L

E
x
S

M
x
G

P

P

W
x
I

-

K

T
x
V

H

N

K

N

A

V

P

N

T
x
Y

Q
x
N

F
x
G

A
x
V

M
|
M

P

P

A

A

F

M

G

-

S

-

R

N

L

L

S

N

D

D

Q

E

E

D

V

I

A

A

E

N

V

V

V

I

T

T

F

F

I

V

R

L

S

N

W

W

G

D

N

N

Q

A

A

G

S

G

S

K

V

V

S

S

A

A

S

E

D

Q

V

V

A

A

K

K

V

Q

R

R

binding protoporphyrin ix containing fe: C347 (= C332), C350 (= C335), H351 (= H336), F362 (= F347), P363 (= P348), P364 (≠ S349), L365 (= L350), S368 (= S353), Y370 (≠ T355), I382 (≠ V368), L386 (≠ A372), S387 (≠ E373), G388 (≠ M374), I390 (≠ W376), V392 (≠ T377), Y397 (≠ T382), N398 (≠ Q383), G399 (≠ F384), V400 (≠ A385), M401 (= M386)

Sites not aligning to the query:

active site: 81, 84, 86, 121, 122, 130, 135, 227, 249, 250, 276
binding copper (ii) ion: 81, 86, 121, 122, 130, 135

5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
33% identity, 33% coverage: 282:427/444 of query aligns to 306:455/456 of 5oboA

query
sites
5oboA

E

E

E

N

D

K

L

L

S

V

A

Y

M

S

A

G

K

K

Y

E

L

L

K

D

S

S

L

V

K

Y

P

L

V

G

K

D

D

R

G

A

T

A

P

P

A

N

L

M

A

S

Y

A

D

V

D

T

K

K

T

A

H

T

Q

Q

A

A

L

S

R

V

K

S

G

G

S

T

-

L

-

T

-

V

-

Q

D

D

Q

Q

-

V

S

Q

P

A

G

G

A

R

M

A

A

L

F

F

L

A

N

G

N
x
T

C
|
C

A

S

A

V

C
|
C

H
|
H

R

Q

S

G

S

N

G

G

K

A

G

G

Y

L

D

P

E

G

T

V

F

F

P
|
P

S
x
P

L
|
L

A

A

L

K

S
|
S

P

D

T
x
F

V

L

N

-

A

A

E

A

N

D

P

P

A

K

S

R

L

A

I

M

R

N

I
|
I

V

V

L

L

E

H

G

G

A

L

E
x
N

M
x
G

P

K

W

I

-

K

T

V

H

N

K

G

A

Q

P

E

T

Y

Q

D

F
x
S

A

V

M
|
M

P

P

A

P

F
x
M

G

-

S

T

R

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

E

N

V

I

V

L

T

T

F

Y

I

V

R

L

S

N

S

S

W

W

G

D

N

N

Q

P

A

G

S

G

S

R

V

V

S

S

A

A

S

E

D

D

V

V

A

K

K

K

V

V

R

R

K

A

Q

Q

binding heme c: T360 (≠ N331), C361 (= C332), C364 (= C335), H365 (= H336), P377 (= P348), P378 (≠ S349), L379 (= L350), S382 (= S353), F384 (≠ T355), I395 (= I367), N401 (≠ E373), G402 (≠ M374), S413 (≠ F384), M415 (= M386), M418 (≠ F389)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitrite ion: 94, 96, 131

6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
33% identity, 33% coverage: 282:427/444 of query aligns to 305:454/455 of 6fjaA

query
sites
6fjaA

E

E

E

N

D

K

L

L

S

V

A

Y

M

S

A

G

K

K

Y

E

L

L

K

D

S

S

L

V

K

Y

P

L

V

G

K

D

D

R

G

A

T

A

P

P

A

N

L

M

A

S

Y

A

D

V

D

T

K

K

T

A

H

T

Q

Q

A

A

L

S

R

V

K

S

G

G

S

T

-

L

-

T

-

V

-

Q

D

D

Q

Q

-

V

S

Q

P

A

G

G

A

R

M

A

A

L

F

F

L

A

N

G

N
x
T

C
|
C

A

S

A

V

C
|
C

H
|
H

R

Q

S

G

S

N

G

G

K

A

G

G

Y

L

D

P

E

G

T

V

F

F

P
|
P

S
x
P

L
|
L

A

A

L

K

S

S

P

D

T
x
F

V

L

N

-

A

A

E

A

N

D

P

P

A

K

S

R

L

A

I

M

R

N

I

I

V

V

L

L

E

H

G

G

A

L

E
x
N

M
x
G

P

K

W

I

-

K

T

V

H

N

K

G

A

Q

P

E

T
x
Y

Q

D

F
x
S

A

V

M
|
M

P

P

A

P

F
x
M

G

-

S

T

R

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

E

N

V

I

V

L

T

T

F

Y

I

V

R

L

S

N

S

S

W

W

G

D

N

N

Q

P

A

G

S

G

S

R

V

V

S

S

A

A

S

E

D

D

V

V

A

K

K

K

V

V

R

R

K

A

Q

Q

binding protoporphyrin ix containing fe: T359 (≠ N331), C360 (= C332), C363 (= C335), H364 (= H336), P376 (= P348), P377 (≠ S349), L378 (= L350), F383 (≠ T355), N400 (≠ E373), G401 (≠ M374), Y410 (≠ T382), S412 (≠ F384), M414 (= M386), M417 (≠ F389)

Sites not aligning to the query:

active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
binding copper (ii) ion: 90, 131, 139, 144

4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
33% identity, 33% coverage: 282:427/444 of query aligns to 308:457/457 of 4ax3D

query
sites
4ax3D

E

E

E

N

D

K

L

L

S

V

A

Y

M

S

A

G

K

K

Y

E

L

L

K

D

S

S

L

V

K

Y

P

L

V

G

K

D

D

R

G

A

T

A

P

P

A

N

L

M

A

S

Y

A

D

V

D

T

K

K

T

A

H

T

Q

Q

A

A

L

S

R

V

K

S

G

G

S

T

-

L

-

T

-

V

-

Q

D

D

Q

Q

-

V

S

Q

P

A

G

G

A

R

M

A

A

L

F

F

L

A

N

G

N

T

C
|
C

A

S

A

V

C
|
C

H
|
H

R

Q

S

G

S

N

G

G

K

A

G

G

Y

L

D

P

E

G

T

V

F

F

P
|
P

S
x
P

L
|
L

A

A

L

K

S
|
S

P

D

T
x
F

V

L

N

-

A

A

E

A

N

D

P

P

A

K

S

R

L

A

I

M

R

N

I

I

V

V

L

L

E

H

G

G

A

L

E
x
N

M
x
G

P

K

W

I

-

K

T

V

H

N

K

G

A

Q

P

E

T

Y

Q

D

F
x
S

A

V

M
|
M

P

P

A

P

F
x
M

G

-

S

T

R

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

E

N

V

I

V

L

T

T

F

Y

I

V

R

L

S

N

S

S

W

W

G

D

N

N

Q

P

A

G

S

G

S

R

V

V

S

S

A

A

S

E

D

D

V

V

A

K

K

K

V

V

R

R

K

A

Q

Q

binding heme c: C363 (= C332), C366 (= C335), H367 (= H336), P379 (= P348), P380 (≠ S349), L381 (= L350), S384 (= S353), F386 (≠ T355), N403 (≠ E373), G404 (≠ M374), S415 (≠ F384), M417 (= M386), M420 (≠ F389)

Sites not aligning to the query:

active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
binding copper (ii) ion: 93, 98, 133, 134, 142, 147

Q03075 Cbb3-type cytochrome c oxidase subunit FixP; Cbb3-Cox subunit FixP; C-type cytochrome FixP; Cyt c(FixP); Cytochrome c oxidase subunit III from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
24% identity, 48% coverage: 197:407/444 of query aligns to 121:285/290 of Q03075

query
sites
Q03075

H

N

C
|
C

G

A

A

P

C
|
C

H

H

T

G

P

S

R

G

G

G

V

A

G

G

M

A

Q

K

E

G

K

F

A

P

L

N

S

L

N

N

D

D

S

D

S

-

Q

D

F

W

L

L

S

W

G

G

V

T

I

L

D

D

G

-

Y

Q

L

I

A

M

N

Q

N

T

L

I

R

Q

G

F

D

G

G

A

R

R

D

S

G

G

L

H

G

A

N

K

W

T

S

H

E

E

A

G

D

Q

I

M

V

L

A

A

F

F

L

G

K

K

T

D

G

G

R

-

N

-

S

-

H

-

S

-

A

-

F

-

G

-

M

-

A

-

D

-

V

-

A

-

N

-

S

-

T

-

Q

-

Y

V

M

L

T

K

E

G

E

D

D

E

L

I

S

V

A

T

M

V

A

A

K

N

Y

Y

L

V

K

R

S

S

L

L

K

S

P

-

V

-

K

-

D

-

G

G

T

L

P

P

A

T

L

-

A

-

Y

-

D

-

K

-

T

-

H

-

Q

-

A

-

L

-

R

R

K

K

G

G

S

Y

D

D

Q

A

S

A

P

K

G

G

A

E

M

K

A

I

F

F

L

V

N

E

N

N

C
|
C

A

V

A

A

C
|
C

H

H

R

G

S

D

S

G

G

G

K

K

G

G

Y

N

D

Q

E

E

T

M

-

G

F

A

P

P

S

N

L

L

A

T

L

D

S

K

P

I

T

W

V

L

N

Y

A

G

E

S

N

D

P

E

A

A

S

A

L

L

I

I

R

E

I

T

V

I

L

S

E

Q

G

G

A

-

E

-

M

-

P

-

W

-

T

-

H

-

K

-

A

-

P

-

T

R

Q

A

F

G

A

V

M

M

P

P

A

A

F

W

G

E

S

G

R

R

L

L

S

D

D

P

Q

S

E

T

V

I

A

K

E

A

V

M

V

A

T

V

F

Y

I

V

R

H

S

S

C122 (= C198) mutation to S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-125 or with S-125, S-219 and S-222. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex.
C125 (= C201) mutation to S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-122 or with S-122, S-219 and S-222. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex.
C219 (= C332) mutation to S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-222 or with S-222, S-122 and S-125. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex.
C222 (= C335) mutation to S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-219 or with S-219, S-122 and S-125. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex.

8ovcC Respiratory supercomplex (iii2-iv2) from mycobacterium smegmatis (see paper)
27% identity, 29% coverage: 279:408/444 of query aligns to 66:181/223 of 8ovcC

query
sites
8ovcC

Y

H

M

F

T

D

E

E

E

S

D

Q

L

I

S

D

A

A

M

L

A

G

K

A

Y

Y

L

V

K

Q

S

A

L

-

K

-

P

-

V

-

K

N

D

G

G

G

T

G

P

P

A

T

L

V

A

P

Y

R

D

D

K

-

T

-

H

H

Q

G

A

A

L

V

R

A

K

Q

-

E

-

S

-

L

-

I

G

G

S

G

D

D

Q

V

S

A

P

R

G

G

A

G

M

D

A

L

F

F

L

R

N

L

N

N

C
|
C

A

A

A

S

C
|
C

H
|
H

R

N

S

F

S

T

G

G

K
|
K

G

G

Y

-

D

-

E

G

T

A

F

L

P

S

S

S

L

G

A

K

L
x
Y

S
x
A

P
|
P

T
x
D

V
x
L

N

G

A

D

E

A

N

N

P

P

A

A

S

Q

L

I

I

Y

R

T

I
x
A

V

M

L

L

E

T

G

G

A

P

E
x
Q

M

-

P

-

W

-

T

-

H

-

K

-

A

-

P

-

T

-

Q

-

F

-

A

N

M
|
M

P

P

A

K

F

F

G

S

S

D

R

R

-

Q

L

L

S

T

D

P

Q

D

E

E

V

K

A

R

E

D

V

I

V

V

T

A

F

Y

I

V

R

R

S

E

S

S

binding heme c: C117 (= C332), C120 (= C335), H121 (= H336), Y135 (≠ L352), A136 (≠ S353), P137 (= P354), L139 (≠ V356), A150 (≠ I367), Q156 (≠ E373), M158 (= M386)
binding acyl-phosphatidyl-myo-inositol dimannoside (AcPIM2): K126 (= K341), D138 (≠ T355)

Sites not aligning to the query:

binding heme c: 15, 16, 19, 20, 32, 41, 44, 45, 50, 51, 52
binding menaquinone-9: 192, 202
binding acyl-phosphatidyl-myo-inositol dimannoside (AcPIM2): 186, 190, 191, 202

6adqC Respiratory complex ciii2civ2sod2 from mycobacterium smegmatis (see paper)
27% identity, 29% coverage: 279:408/444 of query aligns to 66:181/223 of 6adqC

query
sites
6adqC

Y

H

M

F

T

D

E

E

E

S

D

Q

L

I

S

D

A

A

M
x
L

A

G

K

A

Y

Y

L

V

K

Q

S

A

L

-

K

-

P

-

V

-

K

N

D

G

G

G

T

G

P

P

A

T

L

V

A

P

Y

R

D

D

K

-

T

-

H

H

Q

G

A

A

L

V

R

A

K

Q

-

E

-

S

-

L

-

I

G

G

S

G

D

D

Q

V

S

A

P

R

G

G

A

G

M

D

A

L

F

F

L

R

N

L

N

N

C
|
C

A

A

A

S

C
|
C

H
|
H

R

N

S

F

S

T

G

G

K
|
K

G

G

Y

-

D

-

E

G

T

A

F
x
L

P

S

S

S

L

G

A

K

L

Y

S

A

P
|
P

T
x
D

V
x
L

N

G

A

D

E

A

N

N

P

P

A

A

S

Q

L
x
I

I

Y

R

T

I

A

V
x
M

L

L

E

T

G

G

A
x
P

E
x
Q

M

-

P

-

W

-

T

-

H

-

K

-

A

-

P

-

T

-

Q

-

F

-

A

N

M
|
M

P
|
P

A

K

F
|
F

G

S

S

D

R

R

-

Q

L

L

S

T

D

P

Q

D

E

E

V

K

A

R

E

D

V

I

V

V

T

A

F

Y

I

V

R

R

S

E

S

S

binding (2R)-2-(hexadecanoyloxy)-3-{[(S)-hydroxy{[(1R,2R,3R,4R,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9S)-9-methyloctadecanoate: K126 (= K341), D138 (≠ T355)
binding heme c: L75 (≠ M288), C117 (= C332), C120 (= C335), H121 (= H336), L130 (≠ F347), P137 (= P354), L139 (≠ V356), I147 (≠ L364), M151 (≠ V368), P155 (≠ A372), P155 (≠ A372), Q156 (≠ E373), Q156 (≠ E373), M158 (= M386), P159 (= P387), F161 (= F389)

Sites not aligning to the query:

binding (2R)-2-(hexadecanoyloxy)-3-{[(S)-hydroxy{[(1R,2R,3R,4R,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9S)-9-methyloctadecanoate: 182, 186, 189, 190, 191, 202, 206, 207
binding heme c: 16, 19, 20, 32, 34, 37, 41, 42, 44, 45, 50, 51, 52, 53
binding menaquinone-9: 193, 199, 202, 202, 205

Query Sequence

>HSERO_RS16735 FitnessBrowser__HerbieS:HSERO_RS16735
MTTMIKHYFIAACAAVMSLSALTAAAQSNPAAPSADQQLVQRGEYLAKAGDCVACHTAKG
GKPFAGGLAIATPIGTVYSSNITPDKENGIGNYSEEDFDRALRHGIRKDGASLYPAMPYP
SYAKVKPADVKALYAYFMHGVQADPAPNRGVDITWPLSMRWPLSIWRKVFAPAVAVDGPE
DNSPLVRGQYLVEGLGHCGACHTPRGVGMQEKALSNDSSQFLSGGVIDGYLANNLRGDGR
DGLGNWSEADIVAFLKTGRNSHSAAFGGMADVVANSTQYMTEEDLSAMAKYLKSLKPVKD
GTPALAYDDKTHQALRKGSDQSPGAMAFLNNCAACHRSSGKGYDETFPSLALSPTVNAEN
PASLIRIVLEGAEMPWTHKAPTQFAMPAFGSRLSDQEVAEVVTFIRSSWGNQASSVSASD
VAKVRKQLPEKKTVEAGSAPVAKQ

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory