SitesBLAST
Comparing HSERO_RS17015 FitnessBrowser__HerbieS:HSERO_RS17015 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
41% identity, 99% coverage: 3:342/345 of query aligns to 11:351/357 of P27867
3qe3A Sheep liver sorbitol dehydrogenase (see paper)
40% identity, 99% coverage: 3:342/345 of query aligns to 5:345/351 of 3qe3A
- active site: C39 (= C38), G40 (= G39), S41 (= S40), H44 (= H43), H64 (= H63), E65 (= E64), R94 (= R93), D97 (≠ S96), C100 (≠ T99), S108 (≠ D107), F112 (≠ R111), P151 (= P150), G155 (= G154), K339 (≠ D336)
- binding glycerol: Y45 (= Y44), F54 (= F53), T116 (= T115), R293 (= R290)
- binding zinc ion: C39 (= C38), H64 (= H63), E65 (= E64)
1pl6A Human sdh/nadh/inhibitor complex (see paper)
41% identity, 96% coverage: 3:332/345 of query aligns to 10:340/356 of 1pl6A
- active site: C44 (= C38), G45 (= G39), S46 (= S40), H49 (= H43), H69 (= H63), E70 (= E64), R99 (= R93), D102 (≠ S96), C105 (≠ T99), S113 (≠ D107), F117 (≠ R111), P156 (= P150), G160 (= G154)
- binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: C44 (= C38), S46 (= S40), I56 (= I50), F59 (= F53), H69 (= H63), E155 (= E149), L274 (≠ M266), F297 (= F289)
- binding nicotinamide-adenine-dinucleotide: G181 (= G175), P182 (≠ T176), I183 (= I177), D203 (= D197), L204 (≠ V198), R208 (≠ K202), C249 (≠ A241), T250 (≠ S242), V272 (= V264), G273 (= G265), L274 (≠ M266), F297 (= F289), R298 (= R290)
- binding zinc ion: C44 (= C38), H69 (= H63)
Sites not aligning to the query:
Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
41% identity, 96% coverage: 3:332/345 of query aligns to 11:341/357 of Q00796
- C45 (= C38) binding
- H70 (= H63) binding
- E71 (= E64) binding
- R110 (≠ M103) to P: in SORDD; results in protein aggregation
- H135 (= H128) to R: in SORDD; results in protein aggregation
- A153 (= A146) to D: in SORDD; uncertain significance; results in protein aggregation; dbSNP:rs145813597
- I184 (= I177) binding
- D204 (= D197) binding
- R209 (≠ K202) binding
- Q239 (≠ G232) to L: in dbSNP:rs1042079
- N269 (≠ C260) to T: in dbSNP:rs930337
- VGL 273:275 (≠ VGM 264:266) binding
- VFR 297:299 (= VFR 288:290) binding
- V322 (≠ I313) to I: in SORDD; uncertain significance; dbSNP:rs149975952
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
39% identity, 99% coverage: 3:342/345 of query aligns to 9:348/354 of P07846
- C43 (= C38) binding
- Y49 (= Y44) binding
- H67 (= H63) binding
- E68 (= E64) binding
- E153 (= E149) binding
- R296 (= R290) binding
- Y297 (= Y291) binding
1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
39% identity, 98% coverage: 5:341/345 of query aligns to 6:344/348 of 1e3jA
- active site: C38 (= C38), G39 (= G39), S40 (= S40), H43 (= H43), H63 (= H63), E64 (= E64), C93 (≠ R93), C96 (≠ S96), C99 (≠ T99), C107 (≠ D107), T111 (≠ R111), P150 (= P150), G154 (= G154), K341 (= K338)
- binding phosphate ion: A174 (= A174), A196 (= A196), R197 (≠ D197), S198 (≠ V198), R201 (≠ E201)
- binding zinc ion: C38 (= C38), H63 (= H63), E64 (= E64), C93 (≠ R93), C96 (≠ S96), C99 (≠ T99), C107 (≠ D107)
5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
38% identity, 97% coverage: 4:338/345 of query aligns to 8:340/347 of 5vm2A
- active site: C39 (= C38), G40 (= G39), S41 (= S40), H44 (= H43), H65 (= H63), E66 (= E64), C95 (≠ R93), C98 (≠ S96), C101 (≠ T99), C109 (≠ D107), D113 (≠ R111), P153 (= P150), G157 (= G154), K340 (= K338)
- binding magnesium ion: H65 (= H63), E66 (= E64), E152 (= E149)
- binding zinc ion: C95 (≠ R93), C98 (≠ S96), C101 (≠ T99), C109 (≠ D107)
Q7SI09 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 2 papers)
33% identity, 95% coverage: 14:342/345 of query aligns to 29:360/363 of Q7SI09
- C53 (= C38) binding
- F59 (≠ Y44) mutation F->A,S,Y: No effect.
- H78 (= H63) binding
- E79 (= E64) binding
- C108 (≠ R93) binding
- C111 (≠ S96) binding
- C114 (≠ T99) binding
- C122 (≠ D107) binding
- E163 (= E149) binding
- PI 190:191 (≠ TI 176:177) binding
- D211 (= D197) binding
- DI 211:212 (≠ DV 197:198) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-348.
- R216 (≠ K202) binding
- I282 (≠ V264) binding
- QYR 306:308 (≠ VFR 288:290) binding
- S348 (≠ A330) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 211-SR-212.
3m6iA L-arabinitol 4-dehydrogenase (see paper)
33% identity, 95% coverage: 14:342/345 of query aligns to 25:356/358 of 3m6iA
- active site: C49 (= C38), G50 (= G39), S51 (= S40), H54 (= H43), H74 (= H63), E75 (= E64), C104 (≠ R93), C107 (≠ S96), C110 (≠ T99), C118 (≠ D107), D122 (≠ R111), P160 (= P150), A164 (≠ G154), K352 (= K338)
- binding nicotinamide-adenine-dinucleotide: C49 (= C38), V163 (≠ I153), G185 (= G175), P186 (≠ T176), I187 (= I177), D207 (= D197), R212 (≠ K202), C255 (≠ A241), T256 (≠ S242), I278 (≠ V264), G279 (= G265), V280 (≠ M266), R304 (= R290)
- binding zinc ion: C49 (= C38), H74 (= H63), C104 (≠ R93), C107 (≠ S96), C110 (≠ T99), C118 (≠ D107)
7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
35% identity, 96% coverage: 3:333/345 of query aligns to 5:347/357 of 7y9pA
B6HI95 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum) (see paper)
33% identity, 95% coverage: 15:341/345 of query aligns to 31:369/385 of B6HI95
- DI 212:213 (≠ DV 197:198) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-358.
- S358 (≠ A330) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 212-SR-213.
Q96V44 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Hypocrea jecorina (Trichoderma reesei) (see paper)
34% identity, 92% coverage: 27:342/345 of query aligns to 55:374/377 of Q96V44
- DI 224:225 (≠ DV 197:198) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-362.
- A362 (= A330) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 224-SR-225.
A2QAC0 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) (see 2 papers)
33% identity, 95% coverage: 14:341/345 of query aligns to 31:370/386 of A2QAC0
- M70 (≠ F53) mutation to F: Abolishes enzyme activity.
- DI 213:214 (≠ DV 197:198) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-359.
- Y318 (≠ F289) mutation to F: Increases affinity for D-sorbitol.
- A359 (= A330) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 213-SR-214.
P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
29% identity, 98% coverage: 1:337/345 of query aligns to 1:336/341 of P07913
- C38 (= C38) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).
4cpdA Alcohol dehydrogenase tadh from thermus sp. Atn1
32% identity, 95% coverage: 1:327/345 of query aligns to 1:331/346 of 4cpdA
- active site: C38 (= C38), G39 (= G39), S40 (= S40), H43 (= H43), H59 (= H63), E60 (= E64), C89 (≠ R93), C92 (≠ S96), C95 (≠ T99), C103 (≠ D107), G107 (≠ R111), D152 (≠ E149), T156 (≠ I153)
- binding nicotinamide-adenine-dinucleotide: G39 (= G39), S40 (= S40), T156 (≠ I153), G178 (= G175), P179 (≠ T176), V180 (≠ I177), D200 (= D197), R201 (≠ V198), R205 (≠ K202), A243 (= A241), V244 (≠ S242), V266 (= V264), V268 (≠ M266), L292 (= L285), A293 (≠ E286)
- binding zinc ion: C38 (= C38), H59 (= H63), C89 (≠ R93), C92 (≠ S96), C95 (≠ T99), C103 (≠ D107), D152 (≠ E149)
Sites not aligning to the query:
6dkhC The crystal structure of l-idonate 5-dehydrogenase from escherichia coli str. K-12 substr. Mg1655
31% identity, 90% coverage: 30:341/345 of query aligns to 35:344/346 of 6dkhC
2ejvA Crystal structure of threonine 3-dehydrogenase complexed with NAD+
31% identity, 99% coverage: 1:342/345 of query aligns to 1:340/343 of 2ejvA
- active site: C38 (= C38), G39 (= G39), T40 (≠ S40), H43 (= H43), H63 (= H63), E64 (= E64), C93 (≠ R93), C96 (≠ S96), C99 (≠ T99), C107 (≠ D107), Q111 (≠ R111), P149 (= P150), A153 (≠ G154), K336 (= K338)
- binding nicotinamide-adenine-dinucleotide: G172 (= G173), G174 (= G175), P175 (≠ T176), I176 (= I177), S195 (≠ A196), D196 (= D197), P197 (≠ V198), R201 (≠ K202), F238 (≠ A241), S239 (= S242), N241 (≠ H244), A244 (≠ G246), L261 (≠ V264), G262 (= G265), I263 (≠ M266)
- binding zinc ion: C38 (= C38), H63 (= H63), E64 (= E64), C96 (≠ S96), C99 (≠ T99), C107 (≠ D107)
2dq4A Crystal structure of threonine 3-dehydrogenase
31% identity, 99% coverage: 1:342/345 of query aligns to 1:340/343 of 2dq4A
- active site: C38 (= C38), G39 (= G39), T40 (≠ S40), H43 (= H43), H63 (= H63), E64 (= E64), C93 (≠ R93), C96 (≠ S96), C99 (≠ T99), C107 (≠ D107), Q111 (≠ R111), P149 (= P150), A153 (≠ G154), K336 (= K338)
- binding zinc ion: C38 (= C38), H63 (= H63), E64 (= E64), C93 (≠ R93), C96 (≠ S96), C107 (≠ D107)
Q5SKS4 L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
31% identity, 99% coverage: 1:342/345 of query aligns to 1:340/343 of Q5SKS4
- C38 (= C38) binding
- H63 (= H63) binding
- E64 (= E64) binding
- C93 (≠ R93) binding
- C96 (≠ S96) binding
- C99 (≠ T99) binding
- C107 (≠ D107) binding
- I176 (= I177) binding
- D196 (= D197) binding
- R201 (≠ K202) binding
- LGI 261:263 (≠ VGM 264:266) binding
- IAG 286:288 (vs. gap) binding
6ie0B X-ray crystal structure of 2r,3r-butanediol dehydrogenase from bacillus subtilis
31% identity, 92% coverage: 1:317/345 of query aligns to 2:320/347 of 6ie0B
Query Sequence
>HSERO_RS17015 FitnessBrowser__HerbieS:HSERO_RS17015
MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV
LGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWATPPIHG
CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM
TALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQATDGWGADVVFE
ASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYANIFPRALA
LISSGMIDVKPFISRKFPFSQSIRAFEEAASGRPQDVKIQIEMEG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory